Digital Commons Network^™

K12-Biotinylated Histone H4 Marks Heterochromatin In Human Lymphoblastoma Cells, Gabriela Camporeale, Anna M. Oommen, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Covalent modifications of histones play crucial roles in chromatin structure and genomic stability. Recently, we reported a novel modification of histones: biotinylation of lysine residues. Here we provide evidence that K12-biotinylated histone H4 (K12Bio H4) maps specifically to both heterochromatin (alpha satellite repeats in pericentromeric regions) and transcriptionally repressed chromatin (γ-G globin and interleukin-2) in human lymphoblastoma cells. The abundance of K12Bio H4 in these regions was similar to that of K9-dimethylated histone H3, a known marker for heterochromatin. Likewise, K8-biotinylated histone H4 (K8Bio H4) mapped to heterochromatin, but the relative enrichment was smaller compared with K12Bio H4. Stimulation of …

Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Crop residue has been identified as a near-term source of biomass for renewable fuel, heat, power, chemicals and other bio-materials. A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L.) field near Ames, IA. Four harvest scenarios (low cut, high-cut top, high-cut bottom, and normal cut) were evaluated and are expressed as collected stover harvest indices (CSHI). High-cut top and high-cut bottom samples were obtained from the same plot in separate operations. Chemical composition, dilute acid pretreatment response, ethanol conversion yield and efficiency, and thermochemical conversion for each scenario were determined. Mean …

The Chlamydomonas Genome Reveals The Evolution Of Key Animal And Plant Functions, Sabeeha S. Merchant, Simon E. Prochnik, Olivier Vallon, Elizabeth H. Harris, Steven J. Karpowicz, George B. Witman, Astrid Terry, Asaf Salamov, Lillian K. Fritz-Laylin, Laurence Maréchal-Drouard, Wallace F. Marshall, Liang-Hu Qu, David R. Nelson, Anton A. Sanderfoot, Martin H. Spalding, Vladimir V. Kapitonov, Qinghu Ren, Patrick Ferris, Erika Lindquist, Harris Shapiro, Susan M. Lucas, Jane Grimwood, Jeremy Schmutz, Igor V. Grigoriev, Daniel S. Rokhsar, Arthur Grossman, Chlamydomonas Annotation Team, Jgi Annotation Team, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ~120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, …

Center For Grassland Studies Newsletter, Fall 2007, Volume 13, No. 4

Center for Grassland Studies: Newsletters

Contents:

Turfgrass and Landscape Management Degree Program at UNL by Robert (Bob) C. Shearman, Department of Agronomy and Horticulture, UNL

New Farm Bill Should Continue Support for Grassland Management by Duane Hovorka, National Wildlife Federation

Another Great Nebraska Grazing Conference in 2007!

New Ph.D. Specialization in Applied Ecology at UNL by Tala Awada, James Brandle and Larkin Powell, School of Natural Resources, UNL

Nebraska Mentoring Program for Graziers by Roger Chesley, Nebraska Grazing Lands Coalition

2003 National Resources Inventory Information Online

2007 CGS Fall Seminar Series

Forage News [2007-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing Conference
• KY Grazing Conference Hosts Popular Forage Spokesperson Contest
• Grazing Corn Stalks
• Nutrient Losses from Harvested Hay or Crop Residue
• Forages - Louisville - January - Big Event
• Biotechnology in Forage Crops: Update for KY Farmers
• The Effects of Feeding Sericea Lespedeza Hay on Growth Rate of Goats Naturally Infected with Gastrointestinal Nematodes
• Southeastern Drought Drives Search for Hay
• Upcoming Events

Wheat Streak Mosaic Virus Lacking Helper Component-Proteinase Is Competent To Produce Disease Synergism In Double Infections With Maize Chlorotic Mottle Virus, Drake C. Stenger, Brock A. Young, Feng Qu, Thomas Jack Morris, Roy French

Department of Plant Pathology: Faculty Publications

The tritimovirus Wheat streak mosaic virus (WSMV) and the machlomovirus Maize chlorotic mottle virus (MCMV) each cause systemic chlorosis in infected maize plants. Infection of maize with both viruses produces corn lethal necrosis disease (CLND). Here, we report that complete deletion of the WSMV helper component-proteinase (HC-Pro) coding region had no effect on induction of CLND symptoms following coinoculation of maize with WSMV and MCMV. We further demonstrated that elevation of virus titers in double infections, relative to single infections, also was independent of WSMV HC-Pro. Thus, unlike potyvirus HC-Pro, WSMV HC-Pro was dispensable for disease synergism. Because disease synergism …

Nebline, October 2007

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

How Healthy is Your Home?
Soybean Cyst Nematode Spreading in Nebraska
Maintain Grain Quality in Storage
Winterize Your Underground Sprinkler System
Prepare Fireplaces and Woodburning Stoves for Safe Use this Winter
Why Leaves Fall
Choose Decay Resistant Wood for Buildings, Fenceposts
Storing a Tractor
Drying Gourds
Start Garden Cleanup
Tree Planting and Landscape Improvement Funds Available
Grassland Center Sets Fall Lecture Schedule
President’s Notes — Alice’s Analysis
Household Hints
Control Anger Rather Than Suppress It
FCE News & Events
Respond Effectively to Misbehaving Children by Understanding the Problem
Filing Financial Records Saves Time
How Dish Size, Location Influence the Amount …

A Simple Array Platform For Microrna Analysis And Its Application In Mouse Tissues, Xiaoqing Tang, Jozsef Gal, Xun Zhuang, Wang-Xia Wang, Haining Zhu, Guiliang Tang

Plant and Soil Sciences Faculty Publications

MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression at the post-transcriptional level and play a critical role in many important biological processes. Most miRNAs are conserved between humans and mice, which makes it possible to analyze their expressions with a set of selected array probes. Here, we report a simple array platform that can detect 553 nonredundant miRNAs encompassing the entire set of miRNAs for humans and mice. The platform features carefully selected and designed probes with optimized hybridization parameters. Potential cross-reaction between mature miRNAs and their precursors was investigated. The array platform was …

Evaluating The Medicinal Properties Of Mandravasarotra (Cinnamosa Sida) Through Chemical And Pharmacological Screening, Henry J. Bowden Jr.

Independent Study Project (ISP) Collection

I worked for four weeks at the University of Antananarivo (Ankatso), spending two weeks each in the chemistry and pharmacology laboratories. There I ran a total of eight experiments or “screens” with an alcoholic extract of the medicinal plant Mandravasarotra. This plant is found mainly in northwestern Madagascar, and is often used to stimulate contractions for women having difficulty giving birth. The plant tested positive for three secondary metabolites: tannins, steroids and flavanoids. In the pharmacological tests, the plant exhibited anti-inflammatory properties, and high toxicity in doses exceeding 10ml/kg. Together, conjectures may be made between what is known of the …

Lake Mead National Recreation Area Vegetation Monitoring And Analysis: Year-End Progress Report, October 1, 2006 To September 30, 2007, Margaret N. Rees

Vegetation Monitoring

Executive Summary

• The Weed Sentry program surveyed more than 750 miles of federal lands in Clark County for invasive, exotic plant species.
• Weed Sentry also removed more than 600,000 individual invasive plants from federal
  lands. These removals represent pro-active efforts that may have forestalled large
  infestations difficult and costly to eradicate.
• In response to a request by the manager of the Fish and Wildlife Service Desert National
  Wildlife Refuge, a major effort of surveying springs for plant community composition
  and invasive plants provided unique knowledge services by the Weed Sentry program.
  No such baseline information existed for the Sheep Range. …

Comparison Of Laboratory Methods And An In Situ Method For Estimating Nitrogen Mineralization In An Irrigated Silt-Loam Soil, Brian J. Wienhold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen (N) mineralization makes a considerable contribution to crop-available N and is difficult to estimate. Reliable methods for measuring N mineralization are needed to produce data sets for developing N-mineralization models, as a component in fertilizer recommendation algorithms, and to assess the effect of management practices on N mineralization. Numerous methods are available for estimating N mineralization. Laboratory methods are relatively easy but may not reflect conditions in the field, and field methods are usually labor-intensive. A study was conducted to compare N-mineralization estimates using anaerobic and aerobic laboratory methods and an in situ field method for the 0- to …

Tree Windbreaks In The Wheatbelt, Robert Sudmeyer, David Bicknell, Neil Coles

Bulletins 4000 -

Windbreaks comprising trees, or tree and shrub combinations, can offer many benefits on wheatbelt farms, particularly for protection of soil, stock, crops and pastures from damaging winds and erosion. Additional benefits include reduced evaporation from farm dams, reduced groundwater recharge, their use as nature conservation corridors and habitats, increased biodiversity including predators of crop and pasture pests, fire control, tree products and improved lifestyle and aesthetics.

In other areas and farming systems such as intensive horticulture, man-made windbreaks may be used, but their higher cost makes them unsuited for broadscale agriculture. Man-made windbreaks can be effective in reducing evaporative losses …

Forage News [2007-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Spokesman Nominations
• CRP – Forage Quality
• Wet Chemistry Results of Hay Baled from CRP Fields in Hopkins Co. - Aug. 2007
• KFGC Awards
• Stockpiling Tall Fescue
• Cost Per Day on Stockpiled Fescue/Fall Fertilization
• Cost Per Day on Soyhulls/Limit Hay
• Caution When Seeding into Drought Damaged Pastures
• NAFA Summit Promotes Alfalfa for Biofuel
• Consider Annual Ryegrass as a High Quality Forage Option
• Upcoming Events

Nebline, September 2007

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Control Winter Annuals in Fall
Free Soybean Cyst Nematode Sample Kits Available
Free Scrap Tire Collection
Keep Roosting Birds From Dirtying Cars, Driveways This Fall
Wood Heat–A Viable Home Heating Alternative
Ten Rules for Planting Trees
Answers to Composting Questions
President’s Notes — Alice’s Analysis
Household Hints: Programmable Thermostats
FCE News & Events
First Step to Eliminate Debt is Cut Expenses
Ease Children Into Staying Home Alone
Strengthening Family Treasures Daughter/Mother Camp
Four Fruit Salad Recipe
Do You REALLY Know How Much You Eat?
ABC’s for Good Health, Oct. 2, 9 & 23
Free Seminar, “Making Many Meals Using a …

Usda-Ars Lincoln Location Contact Information

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grain, Forage, & Bioenergy Research Unit
Mid-West Livestock Research Unit
Soil & Water Conservation Research Unit
Employee Listing

The mission of the USDA / ARS programs at Lincoln, Nebraska is to improve the productivity, stability of production, sustainability and profitability of crop and livestock production systems in the Great Plains

The Lincoln Location is a part of the Northern Plains Area (NPA) of the Agricultural Research Service (ARS), a Research, Education and Economics (REE) agency, and the main in-house research arm of the United States Department of Agriculture (USDA)

Internode Structure And Cell Wall Composition In Maturing Tillers Of Switchgrass (Panicum Virgatum. L), Gautam Sarath, Lisa M. Baird, Kenneth P. Vogel, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This work examined cell composition gradients in maturing tillers of switchgrass (Panicum virgatuln L. ) with the aim of developing baseline information on this important forage and biomass crop. Flowering tillers were collected from plants raised from seeds in a greenhouse and field, harvested at soil level and separated into internodes beginning with the node subtending the peduncle. Internodes were analyzed using microscopy, by fiber digestion, high-performance liquid chromatography and by gas chromatography-mass spectrometry to obtain anatomical and compositional data. Microscopy demonstrated the development and maturation of cortical fibers which eventually became confluent with the fiber sheath surrounding vascular …

Soil Fertility Management In Organic Vegetable Production, Susannah Rauton

All Theses

Organic vegetable production is a rapidly growing sector of agriculture. Due to limitations on synthetic inputs imposed by the USDA National Organic Program (NOP), research is necessary to determine which soil amendments are viable options for organic farmers. Field trials were conducted at the Clemson University Calhoun Fields Laboratory Student Organic Farm. A variety of vegetable crops were grown, including Jericho lettuce in the springs of 2005 and 2006, yellow crookneck squash in the summers of 2005 and 2006, and Early Jersey Wakefield cabbage in the fall of 2005. Soil amendment treatments consisted of combinations of the following: poultry compost, …

Comparative Genomics Reveals Functional Transcriptional Control Sequences In The Prop1 Gene, Robert D. Ward, Minchul Cho, S. A. Camper, Shannon W. Davis, Lori T. Raetzman, Timothy P.L. Smith, Constance Esposito, Robert H. Lyons, Jan-Fang Cheng, Edward M. Rubin, Simon J. Rhodes

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mutations in PROP1 are a common genetic cause of multiple pituitary hormone deficiency (MPHD). We used a comparative genomics approach to predict the transcriptional regulatory domains of Prop1 and tested them in cell culture and mice. A BAC transgene containing Prop1 completely rescues the Prop1 mutant phenotype, demonstrating that the regulatory elements necessary for proper Prop1 transcription are contained within the BAC. We generated DNA sequences from the PROP1 genes in lemur, pig, and five different primate species. Comparison of these with available human and mouse PROP1 sequences identified three putative regulatory sequences that are highly conserved. These are located …

Nebline, August 2007

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Salute to County Fair Volunteers
Volunteers Needed
Controlling Algae in Ponds and Lakes
Pesticide Container Recycling Program
Larger Diameter Bins Can Save Time and Energy When Drying Grain
Scrap Tire Collection
It’s Time to Take Precautions to Avoid West Nile Virus
Effective Mosquito Repellents
The Limitations of Aerosol Insecticides
Bark Stripping by Fox Squirrels
Walking off Midway Munchies
Skillet Zucchini with Chopped Tomatoes Recipe
Sneak Some Zucchini onto Your Neighbor’s Porch Day
The Power of Colors
President’s Notes — Alice’s Analysis
Household Hints: Cleaning Silver by Electrolysis
FCE News & Events
Back to School Tips
Women & Money Conference, Aug. …

Forage News [2007-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• UK All Commodity Field Day
• 8^th Kentucky Grazing Conference
• Hay Hotline Helps Farmers in Search of Forage
• Beware of Hay Payment Scam
• Judge Amends Roundup Ready Alfalfa Ruling
• Corn Acres Soar, Soy Acres Plunge
• Relative Feed Value (RFV) and Relative Forage Quality (RFQ)
• KFGC Awards
• Forage Spokesman Nominations
• Upcoming Events

Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan

Faculty Publications

Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic …

Growing Olives In Western Australia, Richard Taylor, John Burt

Bulletins 4000 -

There are about 9 million hectares of olives in the world, with the largest areas in Spain, Italy, Greece and Tunisia. Olives are grown between the latitudes of 30° and 45° from the equator. In Australia, the main producing areas are between latitudes 31° and 38°S. Production in Western Australia ranges from Chapman Valley, Northampton (29.5°S), to Albany (34.5°S). Olive production has increased markedly in Australia in the past ten years with a large increase in managed investment scheme olive groves. The Moore River region of the Shire of Gingin accounts for over 70 per cent of olive trees and …

Impacts Of T-Phylloplanin Gene Knockdown And Of Helianthus And Datura Phylloplanins On Peronospora Tabacina Spore Germination And Disease Potential, Antoaneta B. Kroumova, Ryan W. Shepherd, George J. Wagner

Plant and Soil Sciences Faculty Publications

T-phylloplanin proteins secreted to aerial surfaces of tobacco (Nicotiana tabacum) by short procumbent trichomes inhibit spore germination and blue mold disease caused by the oomycete pathogen Peronospora tabacina. Many other plants were found to contain water-washed leaf surface proteins (phylloplanins), but the functions and properties of these are not known. Here we extend earlier evidence for the antifungal activity of T-phylloplanins using a reverse genetics approach. RNA interference of the T-phylloplanin gene in tobacco ‘T.I. 1068’ resulted in loss of T-phylloplanin mRNA and protein, loss of in vitro spore germination inhibition activity, and leaf infection …

Ranunculus Arvensis (Ranunculaceae), An Alien Weed New To Southern California, Richard E. Riefner Jr, Steve Boyd

Aliso: A Journal of Systematic and Floristic Botany

We report the first known records of Ranunculus arvensis from southern California. This alien species shows a tendency to behave as a facultative wetland plant, and could become a pest in scoured washes and flood plains, ephemeral pools, and on disturbed stream banks in the coastal lowlands.

Sheep Updates 2007 - Part 1, Richard Gunner, Anthony Clarke, Kevin Bell, Hugh Dove, H. M. Burrow, Kevin Goss

Sheep Updates

This session covers six papers from different authors:

PLENARY

1. Life beyond the farmgate - the meat perspective, Richard Gunner – Principal:- Richard Gunner’s Fine Meats

2. Do you need to worry about climate change?, Anthony Clark, Centre for Resource and Environmental Studies, Australian National University and Bureau of Rural Sciences.

3. Ruminant nutrition panel session - The impact of nutrition on animal health and welfare, Kevin Bell, School of Veterinary and Biomedical Studies, Murdoch University

4. Ruminant nutrition panel session - Pasture/animal interactions, Hugh Dove, Chief Research Scientist, CSIRO Plant Industry

5. Precision Cattle Breeding for …

Sheep Updates 2007 - Part 3, Andrew Ritchie, Edward Riggall, James Hall, Gus Rose, Johan Greeff, John Young, M. Alchin, M. Young, T. Johnson, John Lucy, Martin Staines, Tim Wiley, Rob Grima, Sandra Prosser, Matt Ryan, Geoff Moore, Tony Albertsen, Phil Barrett-Lennard, George Woolston, John Titerington, Sarah Knight, Brianna Peake

Sheep Updates

This session covers seven papers from different authors:

PROFITABILITY

1. Benchmarking demonstrates both the potential and realised productivity gains in the sheep and wool industry, Andrew Ritchie, Edward Riggall and James Hall, ICON Agriculture, Darkan

2. Improving sheep genetics will increase farm profitability, Gus Rose, Johan Greeff Department of Agriculture and Food Western Australia, John Young Farming Systems Analysis Service, WA

3. Meat, Merinos and making money in WA Pastoral Zone, M. Alchin, M. Young and T. Johnson, Department of Agriculture and Food Western Australia,

GRAZING

4. Nitrogen - farmers' friend or foe? John Lucy and Martin Staines, Department …

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …

Sheep Updates 2007 - Part 5, Bindi Murray, John Karlsson, Johan Greeff, Carolina Viñoles Gil, B. L. Paganoni, K. M. M. Glover, J. T. B. Milton, G. B. Martin, Hugh Dove, Bill Mckiernan, John Wilkins, Paul Greenwood, Linda Cafe

Sheep Updates

This session covers six papers from different authors:

GENETIC IMPROVEMENT

1. Breech Strike Resistance: Selecting for resistance traits reduces breech strike, Bindi Murray, John Karlsson, Johan Greeff, Department of Agriculture and Food, Western Australia

2. Breeding Merino Sheep for Worm Resistance increases profit in a Mediterranean Environment, John Karlsson and Johan Greeff Department of Agriculture and Food, Western Australia

FEEDING

3. Embryo lasses were not increased when Merino ewes that had lost weight were supplemented with lupins, C. Viñoles Gil, B.L. Paganoni, K.M.M. Glover, J.T.B. Milton & G.B. Martin, School of Animal Biology, Faculty of Natural and Agricultural Sciences, …

Flower Pests, Larry A. Sagers

All Archived Publications

No abstract provided.

Center For Grassland Studies Newsletter, Summer 2007, Volume 13, No. 3

Center for Grassland Studies: Newsletters

Contents:

Managing the Meeting of the Cow and the Grass by Pat Reece, Department of Agronomy and Horticulture, Panhandle Research and Extension Center, UNL, Scottsbluff

A Prairie Green Roof by Richard Sutton, Departments of Landscape Architecture and Agronomy and Horticulture, UNL

Carbon Sequestration Pilot Project Wraps Up Steve Chick, USDA - Natural Resources Conservation Service, Lincoln, NE

CGS Council Tours North Central Nebraska

The Nature Conservancy Expands Its Scope

Restored Wetlands Capture Missouri River Water Reducing Flooding