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Chapter 6: Nebraska Experience, S. R. Walker, J. C. Lund, D. G. Schumacher, P. A. Brakhage, B. C. Mcmanus, J. D. Miller, M. M. Augustine, J. J. Carney, R. S. Holland, Kyle D. Hoagland, John C. Holz, Todd M. Barrow, Donald C. Rundquist, Anatoly A. Gitelson Jan 2008

Chapter 6: Nebraska Experience, S. R. Walker, J. C. Lund, D. G. Schumacher, P. A. Brakhage, B. C. Mcmanus, J. D. Miller, M. M. Augustine, J. J. Carney, R. S. Holland, Kyle D. Hoagland, John C. Holz, Todd M. Barrow, Donald C. Rundquist, Anatoly A. Gitelson

Department of Agronomy and Horticulture: Faculty Publications

Nebraska agencies and public health organizations collaboratively addressed cyanobacterial issues for the first time after two dogs died within hours of drinking water from a small private lake south of Omaha on May 4, 2004. A necropsy on one of the dogs revealed that the cause of death was due to ingestion of Microcystin toxins. Within two weeks after the dog deaths, state and local officials jointly developed strategies for monitoring cyanobacterial blooms and issuing public health alerts and advisories. Weekly sampling of public lakes for microcystin toxins and cyanobacteria was initiated during the week of May 17, 2004. ELISA …

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Importance And Effect Of Nitrogen On Crop Quality And Health, Jürg Blumenthal, David D. Baltensperger, Kenneth G. Cassman, Stephen Mason, Alexander Pavlista Jan 2008

Importance And Effect Of Nitrogen On Crop Quality And Health, Jürg Blumenthal, David D. Baltensperger, Kenneth G. Cassman, Stephen Mason, Alexander Pavlista

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) is often the most limiting factor in crop production. Hence, application of fertilizer nitrogen results in higher biomass yields and protein yield and concentration in plant tissue is commonly increased. Nitrogen often affects amino acid composition of protein and in turn its nutritional quality. In cereals, abundant supply of nitrogen decreases the relative proportion of lysine and threonine, thus, reducing the biological value of the protein. Increasing nitrogen supply generally improves kernel integrity and strength, resulting in better milling properties of the grain. In oil seed crops, protein levels are increased upon nitrogen fertilization, whereas oil concentration is …

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Harvester Ant Nests Improve Recovery Performance Of Drought Impacted Vegetation In Grazing Regimes Of Semiarid Savanna, Texas, Nancy Nicolai, Fred Smeins, Jerry Cook Jan 2008

Harvester Ant Nests Improve Recovery Performance Of Drought Impacted Vegetation In Grazing Regimes Of Semiarid Savanna, Texas, Nancy Nicolai, Fred Smeins, Jerry Cook

Department of Agronomy and Horticulture: Faculty Publications

Vegetation composition is often dictated by grazing intensity in semiarid savannas; recovery following drought may depend on pre drought species composition. Nests of the red harvester ant, Pogonomyrmex barbatus, affect the dynamics, composition and recovery of post drought communities due to their larger size, greater seed production and higher perennial grass richness. We hypothesized that vegetation at ant nests would survive drought and recover faster than vegetation in the surrounding grasslands, but that individual and population recovery would depend on plant species composition, which, in turn, would be influenced by grazing intensity. We assessed nest influence on density, cover, …

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Characterization Of The Folate Salvage Enzyme P-Aminobenzoylglutamate Hydrolase In Plants, Gale G. Bozzo, Gilles J. C. Basset, Valeria Naponelli, Alexandre Noiriel, Jesse F. Gregory Iii, Andrew D. Hanson Jan 2008

Characterization Of The Folate Salvage Enzyme P-Aminobenzoylglutamate Hydrolase In Plants, Gale G. Bozzo, Gilles J. C. Basset, Valeria Naponelli, Alexandre Noiriel, Jesse F. Gregory Iii, Andrew D. Hanson

Department of Agronomy and Horticulture: Faculty Publications

Folates break down in vivo to give pterin and p-aminobenzoylglutamate (pABAGlu) fragments, the latter usually having a polyglutamyl tail. Pilot studies have shown that plants can hydrolyze pABAGlu and its polyglutamates to p-aminobenzoate, a folate biosynthesis precursor. The enzymatic basis of this hydrolysis was further investigated. pABAGlu hydrolase activity was found in all species and organs tested; activity levels implied that the proteins responsible are very rare. The activity was located in cytosol/vacuole and mitochondrial fractions of pea (Pisum sativum L.) leaves, and column chromatography of the activity from Arabidopsis tissues indicated at …

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Detection And Quantification Of Vitamin K1 Quinol In Leaf Tissues, Chloë Van Oostende, Joshua R. Widhalm, Gilles J.C. Basset Jan 2008

Detection And Quantification Of Vitamin K1 Quinol In Leaf Tissues, Chloë Van Oostende, Joshua R. Widhalm, Gilles J.C. Basset

Department of Agronomy and Horticulture: Faculty Publications

Phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone; vitamin K1) is vital to plants. It is responsible for the oneelectron transfer at the A1 site of photosystem I, a process that involves turnover between the quinone and semi-quinone forms of phylloquinone. Using HPLC coupled with fluorometric detection to analyze Arabidopsis leaf extracts, we detected a third redox form of phylloquinone corresponding to its fully reduced – quinol–naphthoquinone ring (PhQH2). A method was developed to quantify PhQH2 and its corresponding oxidized quinone (PhQ) counterpart in a single HPLC run. PhQH2 was found in leaves of all dicotyledonous and monocotyledonous species tested, …

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Geographic Patterns Of At-Risk Species, Curtis H. Flather, Michael S. Knowles, Jason Mcnees Jan 2008

Geographic Patterns Of At-Risk Species, Curtis H. Flather, Michael S. Knowles, Jason Mcnees

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

This technical document supports the Forest Service’s requirement to assess the status of renewable natural resources as mandated by the Forest and Rangeland Renewable Resources Planning Act of 1974. It updates past reports on the trends and geographic patterns of species formally listed as threatened or endangered under the Endangered Species Act of 1973. We compare the geographic occupancy of threatened and endangered species at the county-level against the geographic occupancy of a broader set of species thought to be at risk of extinction. This is done to determine if new areas where species rarity may be concentrated emerge. Here …

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Asas Centennial Paper: Net Energy Systems For Beef Cattle – Cattles, Application, And Future Models, Calvin Ferrell, J. W. Oltjen Jan 2008

Asas Centennial Paper: Net Energy Systems For Beef Cattle – Cattles, Application, And Future Models, Calvin Ferrell, J. W. Oltjen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Development of nutritional energetics can be traced to the 1400s. Lavoisier established relationships among O2 use, CO2 production and heat production in the late 1700s, and the laws of thermodynamics and law of Hess were discovered during the 1840s. Those discoveries established the fundamental bases for nutritional energetics and enabled the fundamental entity ME = retained energy + heat energy to be established. Objectives became: 1) to establish relationships between gas exchange and heat energy, 2) to devise bases for evaluation of foods that could be related to energy expenditures, and 3) to establish causes of energy expenditures. …

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A Putative Quantitative Trait Locus On Chromosome 20 Associated With Bovine Pathogenic Disease Incidence, E. Casas, G. Snowder Jan 2008

A Putative Quantitative Trait Locus On Chromosome 20 Associated With Bovine Pathogenic Disease Incidence, E. Casas, G. Snowder

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The objective of this study was to detect QTL associated with the incidence of multiple pathogenic diseases in offspring from half-sib bovine families. Four F1 sires were used to produce offspring: Brahman × Hereford (BH; n = 547), Piedmontese × Angus (PA; n = 209), Brahman × Angus (n = 176), and Belgian Blue × MARC III (n = 246). Treatment records for bovine respiratory disease, infectious keratoconjunctivitis (pinkeye), and infectious pododermatitis (footrot) were available for all of the offspring from birth to slaughter. The incidences of these 3 microbial pathogenic diseases were combined into a single binary trait …

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Chapter 18. Daycent Simulated Effects Of Land Use And Climate On County Level N Loss Vectors In The Usa, S. J. Del Grosso, W. J. Parton, D. S. Ojima, C. A. Keough, T. H. Riley, A. R. Mosier Jan 2008

Chapter 18. Daycent Simulated Effects Of Land Use And Climate On County Level N Loss Vectors In The Usa, S. J. Del Grosso, W. J. Parton, D. S. Ojima, C. A. Keough, T. H. Riley, A. R. Mosier

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We describe the nitrogen (N) gas (NH3, NOx, N2O, N2) emission and NO3 leaching submodels used in the DAYCENT ecosystem model and demonstrate the ability of DAYCENT to simulate observed N2O emission and NO3 leaching rates for various sites representing different climate regimes, soil types, and land uses. DAYCENT simulated seven major crops, grazing lands, and potential native vegetation at the county level for the United States. At the national scale, NO3 leaching was the major loss vector, accounting for 86%, 66%, and 56% of total N …

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Chapter 15. Proven Practices And Innovative Technologies For On-Farm Crop Nitrogen Management, N. R. Kitchen, K. W. T. Goulding, J.F. Shanahan Jan 2008

Chapter 15. Proven Practices And Innovative Technologies For On-Farm Crop Nitrogen Management, N. R. Kitchen, K. W. T. Goulding, J.F. Shanahan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen (N) from soil, fertilizer, and manure sources is generally inefficiently used (30-60%) in most crop production systems. As a consequence, unused inorganic N can move off crop fields and contaminate surface and groundwater resources. Local and national governments have responded with guidelines, standards, regulations, and in some cases fines when off-field losses of N have not been reduced. Along with these environmental pressures, soaring energy costs have resulted in commensurate increased costs for N fertilizers. These factors are real for crop producers and are compelling them to scrutinize their crop N management more closely than in previous decades. Numerous …

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Chapter 4. Relationship Of Environmental Nitrogen Metabolism To Human Health, J. R. Follett, R. F. Follett Jan 2008

Chapter 4. Relationship Of Environmental Nitrogen Metabolism To Human Health, J. R. Follett, R. F. Follett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The need for humans to produce and consume food and other agricultural products is increasing. This need is directly related to increasing world populations, demands for goods and services, and expectations. Nitrogen (N) is contained in all of the amino acids and proteins in the foods consumed by humans. The use of N to produce food and other products is generally increasing as human needs increase. Proteins are an essential component of the human diet because, unlike plants, humans are unable to utilize more simple forms of N and rely on food sources for protein which can then be digested …

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Effect Of Sample Processing Procedures On Measurement Of Starch In Corn Silage And Corn Grain, M. B. Hall, D. R. Mertens Jan 2008

Effect Of Sample Processing Procedures On Measurement Of Starch In Corn Silage And Corn Grain, M. B. Hall, D. R. Mertens

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Methods for processing feedstuffs before analysis can affect analytical results. Effects of drying temperature (corn silage), preservation method (corn grain), and grinding method (corn silage and grain) on starch analysis values were evaluated. Corn silage samples dried at 55 or 105°C and grain samples dried at 55°C were ground to pass the 1-mm screen of an abrasion mill or cutting mill and analyzed for free glucose and starch corrected for free glucose. Starch analyses were performed in triplicate to assess the effect of treatment on precision of starch determination. Drying at 105°C decreased free glucose and tended to decrease starch …

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Growth And Feed Efficiency Of Juvenile Channel Catfish Reared At Different Water Temperatures And Fed Diets Containing Various Levels Of Fish Meal, Menghe H. Li, Edwin H. Robinson, Brian C. Peterson, Terry D. Bates Jan 2008

Growth And Feed Efficiency Of Juvenile Channel Catfish Reared At Different Water Temperatures And Fed Diets Containing Various Levels Of Fish Meal, Menghe H. Li, Edwin H. Robinson, Brian C. Peterson, Terry D. Bates

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Channel catfish Ictalurus punctatus do not feed well at low temperatures. It is generally thought that a diet containing fish meal enhances feed palatability at low temperatures since fish meal is highly palatable to fish. There is a lack of information on the effects of fish meal levels on the growth performance of channel catfish reared at low temperatures. Therefore, a study was conducted in a recirculating system to examine the effects of fish meal levels on the feed consumption, weight gain, and feed efficiency of juvenile channel catfish reared at various temperatures. Fish with an initial weight of 9.6 …

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Areawide Suppression Of Fire Ants, M. D. Aubuchon, R. K. Vander Meer Jan 2008

Areawide Suppression Of Fire Ants, M. D. Aubuchon, R. K. Vander Meer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The imported fire ants, Solenopsis invicta and S. richteri, were inadvertently introduced into the USA in the early 1900s and currently inhabit over 129 million ha in Puerto Rico and 12 southern states, from Texas to Virginia (Callcott and Collins, 1996; USDA-APHIS map). Imported fire ants have also become established in isolated sites in California, Arizona, New Mexico and Maryland. Strict quarantine procedures have limited the spread of this pest (Lockley and Collins, 1990), but eventually populations will expand westward in increasing numbers in New Mexico, Arizona and California. They will also move upward along the Pacific coast, southward …

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Aged Switchgrass Seed Lot's Response To Dormancy-Breaking Chemicals, Gautam Sarath, Robert B. Mitchell Jan 2008

Aged Switchgrass Seed Lot's Response To Dormancy-Breaking Chemicals, Gautam Sarath, Robert B. Mitchell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aged switchgrass seed lots can display various levels of dormancy. Under-standing the causes for this dormancy will provide better insight into seed physiology, and potentially lead to treatments that reduce variability in seed germination assays. The effects of sodium nitroprusside, potassium ferro-cyanide and hydrogen peroxide on the germination of eight aged switch-grass (Panicum virgatum L.) seed lots, seven of which were produced in the same year at a single experiment station, were examined. Seed germination demonstrated a significant seed lots × days and treatments × days effect. However, responses of individual seed lots to specific chemicals varied considerably. Genetically …

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Screwworms, Cochliomyia Hominivorax, Reared For Mass Release Do Not Carry And Spread Foot-And-Mouth Disease Virus And Classical Swine Fever Virus, M. F. Chaudhury, G. B. Ward, Steven R. Skoda, M. Y. Deng, J. B. Welch, T. S. Mckenna Jan 2008

Screwworms, Cochliomyia Hominivorax, Reared For Mass Release Do Not Carry And Spread Foot-And-Mouth Disease Virus And Classical Swine Fever Virus, M. F. Chaudhury, G. B. Ward, Steven R. Skoda, M. Y. Deng, J. B. Welch, T. S. Mckenna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Experiments were done to determine if transporting live screwworms Cochliomyia hominivorax Coquerel (Diptera: Calliphoridae) for developing new strains from countries where foot-and-mouth disease and classical swine fever are endemic, to the mass rearing facilities in Mexico and Panama, may introduce these exotic diseases into these countries. Are screwworms capable of harboring and spreading foot-and-mouth disease virus (FMDV) and classical swine fever virus (CSFV) when they are grown in virus-inoculated larval rearing medium? In one experiment, screwworm larvae were reared in a FMDV-inoculated artificial medium containing either 0.1% formaldehyde. In each experiment, samples of larvae and the rearing media were collected …

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Volatile Compounds From The Integument Of White Leghorn Chickens (Gallus Gallus Domesticus L.): Candidate Attractants Of Ornithophilic Mosquito Species, Ulrich R. Bernier, Sandra A. Allan, Brian Quinn, Daniel Kline, Donald Barnard, Gary Clark Jan 2008

Volatile Compounds From The Integument Of White Leghorn Chickens (Gallus Gallus Domesticus L.): Candidate Attractants Of Ornithophilic Mosquito Species, Ulrich R. Bernier, Sandra A. Allan, Brian Quinn, Daniel Kline, Donald Barnard, Gary Clark

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Candidate kairomones of ornithophilic mosquito species are reported from GC/MS analysis of compounds from the skin on the back, the feet, and feathers from the back of White Leghorn chickens (Gallus gallus domesticus). Hexane and ether extracts of chicken feathers differ significantly in attractiveness of Culex spp. mosquitoes. The active (hexane) extracts contained an abundance of alcohols, ketones, and diones. The inactive (ether) extracts contained aldehydes, which also were in the hexane extracts. Analysis of hexane extracts from chicken feet, skin, and feathers demonstrated the qualitative similarity in the compounds collected with subtle differences observed in the quantitative …

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Assessing Yield Potential Of Cowpea Genotypes Grown Under Virus Pressure, Ricardo Goenaga, Adolfo Quiles, A. Graves Gillaspie Jan 2008

Assessing Yield Potential Of Cowpea Genotypes Grown Under Virus Pressure, Ricardo Goenaga, Adolfo Quiles, A. Graves Gillaspie

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cowpea or Southernpea [Vigna unguiculata (L.) Walp.] is an important grain legume in many parts of the tropics. However, viral diseases, particularly Cucumber mosaic virus (CMV) and Blackeye cowpea mosaic virus (BlCMV), can be a limiting factor in cowpea production. We evaluated in replicated field plots and under virus pressure nine PIs (441919, 441925, 441917, 147071, 146618, 180014, 180355, 194208, 612607) and three commercial cultivars (Coronet, KnuckleHull-VNR, Pinkeye Purplehull), some of which had shown absence of symptoms for CMV and BlCMV in unreplicated, seed regeneration plots of the U.S. cowpea collection. Only 3% of all plots had plants infected …

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Discovery Of Diurnal Resting Sites Of Phlebotomine Sand Flies In A Village In Southern Egypt, Jerome Hogsette, Hanafi A. Hanafi, Ulrich R. Bernier, Daniel L. Kline, Emad Y. Fawaz, Barry D. Furman, David F. Hoel Jan 2008

Discovery Of Diurnal Resting Sites Of Phlebotomine Sand Flies In A Village In Southern Egypt, Jerome Hogsette, Hanafi A. Hanafi, Ulrich R. Bernier, Daniel L. Kline, Emad Y. Fawaz, Barry D. Furman, David F. Hoel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In an attempt to find diurnal resting sites of adult phlebotomine sand flies, potential phlebotomine adult habitats were aspirated in the village of Bahrif in Aswan, Egypt. During this survey, sand flies were aspirated from low (30–45 cm high) irregular piles of mud bricks found under high date palm canopies between the village and the Nile River. There were 5 ♂♂and 7 ♀♀ of Phlebotomus papatasi and 3♂♂ of Sergentomyia schwetzi. Six of the 7 aspirated females were engorged with blood. A total of 78 sand flies was captured on 3 glue boards placed overnight on the ground next …

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Improvement Of Pvx/Cmv Cp Expression Tool For Display Of Short Foreign Antigens, Angela Natilla, Lev G. Nemchinov Jan 2008

Improvement Of Pvx/Cmv Cp Expression Tool For Display Of Short Foreign Antigens, Angela Natilla, Lev G. Nemchinov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We have previously reported that Potato virus X-expressed coat protein of Cucumber mosaic virus (CMV) formed virus-like particles (VLPs), which served as carriers for display of different neutralizing epitopes of Newcastle disease virus (NDV). In this work, we further modified the purification protocol of recombinant VLPs carrying short neutralizing epitopes of the NDV proteins and demonstrated that self-contained capsid protein subunits of CMV transiently expressed from heterologous virus packaged into individual virions morphologically resembling and/or indistinguishable from wild type CMV particles. Homogeneity of the final preparation represents an advance over our previous study, where VLPs were found to be of …

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The Genetic Diversity, Relationships, And Potential For Biological Control Of The Lobate Lac Scale, Paratachardina Pseudolobata Kondo & Gullan (Hemiptera: Coccoidea: Kerriidae), S. Schroer, R. W. Pemberton, L. G. Cook, T. Kondo, P. J. Gullan Jan 2008

The Genetic Diversity, Relationships, And Potential For Biological Control Of The Lobate Lac Scale, Paratachardina Pseudolobata Kondo & Gullan (Hemiptera: Coccoidea: Kerriidae), S. Schroer, R. W. Pemberton, L. G. Cook, T. Kondo, P. J. Gullan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The lobate lac scale Paratachardina pseudolobata Kondo & Gullan (Kerriidae) is a polyphagous pest of woody plants in Florida (U.S.A.) the Bahamas, Christmas Island (Australia) and it has been reported from Cuba. Its recent appearance as a pest in these places indicates that this scale is introduced; however, its native range is unknown. Until 2006, this pest species was identified mistakenly as Paratachardina lobata (Chamberlin) [now P. silvestri (Mahdihassan)], which is native to India and Sri Lanka. Quarantine laboratory acceptance trials with Indian P. silvestri parasitoids indicated a strong immune response from P. pseudolobata. Gregarious development of encyrtid wasps …

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A Core Set Of Microsatellite Markers For Western Corn Rootworm (Coleoptera: Chrysomelidae) Population Genetics Studies, Kyung Seok Kim, Uwe Stolz, Nicholas J. Miller, Miller R. Waits, Thomas Guillermaud, Douglas V. Sumerford, Thomas W. Sappington Jan 2008

A Core Set Of Microsatellite Markers For Western Corn Rootworm (Coleoptera: Chrysomelidae) Population Genetics Studies, Kyung Seok Kim, Uwe Stolz, Nicholas J. Miller, Miller R. Waits, Thomas Guillermaud, Douglas V. Sumerford, Thomas W. Sappington

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in the ecological and population genetics of the western corn rootworm, Diabrotica virgifera virgifera LeConte, has grown rapidly in the last few years in North America and Europe. This interest is a result of a number of converging issues related to the increasing difficulty in managing this pest and the need to characterize and understand gene ßow in the context of insect resistance management. One of the key components needed for successful population genetics studies is the availability of suitable molecular markers. Using a standard group of microsatellite markers enables researchers from different laboratories to directly compare and share …

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Prevalence Of The Prion Protein Gene E211k Variant In U.S. Cattle, Michael P Heaton, John W. Keele, Gregory P. Harhay, Jürgen A. Richt, Mohammad Koohmaraie, Tommy L. Wheeler, Steven D. Shackelford, Eduardo Casas, D. Andy King, Tad S. Sonstegard, Curtis P. Van Tassell, Holly L. Neibergs, Chad C. Chase Jr., Theodore S. Kalbfleisch, Timothy Smith, Michael L. Clawson, William W. Laegreid Jan 2008

Prevalence Of The Prion Protein Gene E211k Variant In U.S. Cattle, Michael P Heaton, John W. Keele, Gregory P. Harhay, Jürgen A. Richt, Mohammad Koohmaraie, Tommy L. Wheeler, Steven D. Shackelford, Eduardo Casas, D. Andy King, Tad S. Sonstegard, Curtis P. Van Tassell, Holly L. Neibergs, Chad C. Chase Jr., Theodore S. Kalbfleisch, Timothy Smith, Michael L. Clawson, William W. Laegreid

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: In 2006, an atypical U.S. case of bovine spongiform encephalopathy (BSE) was discovered in Alabama and later reported to be polymorphic for glutamate (E) and lysine (K) codons at position 211 in the bovine prion protein gene (Prnp) coding sequence. A bovine E211K mutation is important because it is analogous to the most common pathogenic mutation in humans (E200K) which causes hereditary Creutzfeldt – Jakob disease, an autosomal dominant form of prion disease. The present report describes a high-throughput matrix-associated laser desorption/ionization-time-of-flight mass spectrometry assay for scoring the Prnp E211K variant and its use to determine an upper limit …

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Reestablishing The Juneberry On The Fort Berthold Indian Reservation: Cultural, Horticultural, And Educational Connections, Kerry E. Hartman Jan 2008

Reestablishing The Juneberry On The Fort Berthold Indian Reservation: Cultural, Horticultural, And Educational Connections, Kerry E. Hartman

Electronic Theses and Dissertations

Tribal people of the Northern Great Plains have utilized plants for centuries. Amelanchier anifolia (Juneberries/Serviceberries) historically played an important part in the diet and culture of the Mandan, Hidatsa, and Arikara (MHA) Tribal Nations. Research conducted as part of this study into historical and contemporary uses of the Juneberry by MHA Tribal Members indicated extensive historical use and a high interest in Juneberry reestablishment for cultural, nutritional, and economic reasons. Previous research on Juneberries has investigated factors including state of dormancy, propagation method, transplant type, and mulch type. Another purpose of this study was to elucidate the impact of presence …

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New Strategy For The Search Of Natural Biologically Active Substances., Vyacheslav Dushenkov, Ilya Raskin Jan 2008

New Strategy For The Search Of Natural Biologically Active Substances., Vyacheslav Dushenkov, Ilya Raskin

Publications and Research

The modern pharmaceutics actively screens an immense diversity of substances occurring in plants and other natural resources in the search for new effective medicinal agents. The Global Institute for Bioexploration (GIBEX) established by joint efforts of Rutgers University and the University of Illinois (United States) represents the organizational core of international scientific community whose activity is directed towards the search and development of new medicinal preparations from natural raw materials. The basis of GIBEX activity is the transfer of modern screening technologies to countries and geographical regions characterized by remarkable biodiversity. The GIBEX goals are to encourage the search for …

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Removal Of Manganese From An Alkaline Mine Drainage Using A Bioreactor With Different Organic Carbon Sources, Jared D. Edwards Jan 2008

Removal Of Manganese From An Alkaline Mine Drainage Using A Bioreactor With Different Organic Carbon Sources, Jared D. Edwards

University of Kentucky Master's Theses

The treatment of Mn and SO42- contaminated mine drainage via a sulfate reducing bioreactor is expected to result in near-permanent immobilization of significant amounts of Mn and a portion of the sulfates within the matrix. This study tested several different combinations of organic amendments and inorganic substrates in an attempt to optimize sulfate reducing conditions and Mn removal capacity. Five different organic carbon sources, including corn mash, wood mulch, biosolids, soybean oil, and sorghum syrup in combination with five different inorganic substrates, including creek sediment, marble and limestone chips, polished gravel, and sand were tested in batch experiments. …

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Assessment Of Terrain Attribute Models For The Identification Of Erosion Prone Areas Suitable For The Establishment Of Grassed Waterways In An Agricultural Field Setting In The Out Bluegrass Region Of Kentucky, Adam Clellon Pike Jan 2008

Assessment Of Terrain Attribute Models For The Identification Of Erosion Prone Areas Suitable For The Establishment Of Grassed Waterways In An Agricultural Field Setting In The Out Bluegrass Region Of Kentucky, Adam Clellon Pike

University of Kentucky Master's Theses

The speed and accuracy of conservation planning could be improved if maps indicating areas where grassed waterways should be placed to reduce erosion could be easily created. For five central Kentucky fields, elevation data were obtained with real time kinematic (RTK) global positioning system (GPS) and from US Geological Survey (USGS) digital elevation models (DEMs). Terrain attributes were calculated from these datasets which were used as predictor variables for neural network and logistic regression analyses. Grassed waterway prediction models were developed with these analyses. The type of activation function, type of standardization procedure, number of neurons, number of preliminary runs, …

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Performance And Physiology Of Yearling Steers Grazing Toxic Tall Fescue As Influenced By Concentrate Feeding And Steroidal Implants, Jessica Meagan Carter Jan 2008

Performance And Physiology Of Yearling Steers Grazing Toxic Tall Fescue As Influenced By Concentrate Feeding And Steroidal Implants, Jessica Meagan Carter

University of Kentucky Master's Theses

Fescue toxicosis can produce negative effects on animal weight gain and physiology. Sixty-four steers were grazed on endophyte-infected (E+) KY-31 tall fescue for 77 days in 2007 and sixty steers grazed for 86 days in 2008 to evaluate interactions with implantation of steroidal implants and concentrate feeding on performance and physiology of yearling steers. Steers were stratified by body weight for assignment to six, 3.0-ha toxic tall fescue pastures. The main plot treatment of with or without pelleted soybean hulls (SBH) were randomly assigned to pastures. Pelleted SBH were group-fed to provide daily consumptions of 2.3 kg/steer/d (as fed). Sub-plot …

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Roles Of Micrornas In Plant Abiotic Stress, Development And Viral Infection, Venugopal Mendu Jan 2008

Roles Of Micrornas In Plant Abiotic Stress, Development And Viral Infection, Venugopal Mendu

University of Kentucky Doctoral Dissertations

Plant microRNAs play important roles in plant growth and development. Here we investigated the roles of miRNAs in the plant abiotic stress, development and viral infection. MicroRNA membrane array analysis using five different abiotic stress treatments resulted in the identification of 8 novel stress inducible miRNA-families. Functional studies on novel stress inducible miR168 revealed its functional relation with abiotic stress. Over expression of miR168 in Arabidopsis showed upregulation of four stress related miRNAs (miR163, miR167, miR398 and miR408). Analysis of 9 independent transgenic lines showed induction of miR398, an oxidative stress responsive miRNA with a corresponding down regulation of its …

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Molecular Diversity And Coat Protein Expression Of Sweet Potato Leaf Curl Virus, Dina Lida Gutierrez Reynoso Jan 2008

Molecular Diversity And Coat Protein Expression Of Sweet Potato Leaf Curl Virus, Dina Lida Gutierrez Reynoso

LSU Doctoral Dissertations

Leaf curl virus diseases have been reported in sweetpotato throughout the world. One of the causal agents is Sweet potato leaf curl virus (SPLCV) which belongs to the genus Begomovirus (family Geminiviridae). In the United States, SPLCV has been found infecting an ornamental sweetpotato and several breeding lines but not in sweetpotatoes grown for commercial production. SPLCV does not cause symptoms on Beauregard, the predominant sweetpotato cultivar in the US, but it can reduce its yield. Since SPLCV could become an important constraint for sweetpotato production; diagnosis, identification, and characterization are essential steps to develop an effective management program. The …

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