Digital Commons Network^™

Protein-Protein Interactions Of Tandem Affinity Purified Protein Kinases From Rice, Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald L. Cerny, Christopher Dardick, Patrick Canlas, Hiroaki Fujii, Michael Gribskov, Siddhartha Kanrar, Lucas Knoflicek, Becky Stevenson, Mingtang Xie, Xia Xu, Xianwu Zheng, Jing-Kang Zhu, Pamela Ronald, Michael E. Fromm

Center for Plant Science Innovation: Faculty Publications

Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAPtagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 …

Intergenic Transcription By Rna Polymerase Ii Coordinates Pol Iv And Pol V In Sirna-Directed Transcriptional Gene Silencing In Arabidopsis, Binglian Zheng, Zhengming Wang, Shengben Li, Bin Yu, Jin -Yuan Liu, Xuemei Chen

Center for Plant Science Innovation: Faculty Publications

Intergenic transcription by RNA Polymerase II (Pol II) is widespread in plant and animal genomes, but the functions of intergenic transcription or the resulting noncoding transcripts are poorly understood. Here, we show that Arabidopsis Pol II is indispensable for endogenous siRNA-mediated transcriptional gene silencing (TGS) at intergenic low-copy-number loci, despite the presence of two other polymerases—Pol IV and Pol V—that specialize in TGS through siRNAs. We show that Pol II produces noncoding scaffold transcripts that originate outside of heterochromatic, siRNA-generating loci. Through these transcripts and physical interactions with the siRNA effector protein ARGONAUTE4 (AGO4), Pol II recruits AGO4/siRNAs to homologous …

The Impacts Of Global Warming On Appalachian Wildflower Phenologies, Rachel D. Wigginton

Mahurin Honors College Capstone Experience/Thesis Projects

Public and private interest in global warming has prompted exploration of the impacts this phenomenon may impart on ecosystem functions. Flowering phenology has been one of the areas many scientists believe is particularly susceptible to the impacts of anthropogenic warming. Over three weekends in spring of 2008, the vernal herb community was surveyed at five sites within the Great Smoky Mountains regions of the southern Appalachian Mountains. The intent was to capture the naturally occurring elevational gradient and determine if the temperature cue for blooming was the same for all co-flowering species in the study. This information would allow for …

Agroforestry: Working Trees For Sequestering Carbon On Agricultural Lands, Michele M. Schoeneberger

Working Trees (USDA-NAC)

No abstract provided.

Contents, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Post Weaning Management Of Heifer Calves Impacts Average Daily Gain And Feed Efficiency As Pregnant Heifers, D. M. Larson, Tonya L. Meyer, L. A. Stalker, Richard N. Funston

West Central Research and Extension Center, North Platte

Three experiments evaluated replacement heifer development systems and subsequent effects on gain and efficiency of pregnant heifers. In Exp. 1 and 2, were developed grazing corn residue (CR; 422 ± 5 kg) or fed in a dry lot (DL; 446 ± 5 kg) prior to breeding. In Exp. 1, a subset of pregnant heifers (n = 40) were individually fed a diet composed of 90% grass hay (11.7 % CP; DM basis) and 10% of a wet distillers grains plus solubles/straw mixture (21.8 % CP; DM basis) during late gestation. In Exp. 2, 55 pregnant …

Nebline, January 2009

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Creating a Strong Family: Strong Families Have Six Common Characteristics
Ways to Spend Time With Your Family in January
Private Pesticide Applicator Certification Opportunities
Soybean Cyst Nematode Spreading in Nebraska: Free Soil Sampling Kits at Extension Offices
Mystery “Bites”: Unidentifiable Skin Problems Usually Have Non-Insect Causes
Raccoons in the City
2009 Wall Calendar
Beginning Beekeeping 2-Day Workshop
2009 All-America Rose Selections
Shopping for Plants and Seeds
Garden Guide: Things to Do This Month
Birds Need Water In Winter
Attract Winter Birds With Feeders
Top 10 Myths of Entrepreneurship
Winter Houseplant Care
Wishing You a Healthy H-A-P-P-Y N-E-W Y-E-A-R!
$tretch Your …

The Prairie Naturalist Manuscript Submission Guidelines, Christopher N. Jacques, Troy W. Grovenburg, Jonathan Jenks

The Prairie Naturalist

These guidelines present The Prairie Naturalist (PNAT) policies and procedures for submitting scientific manuscripts for consideration for publication. In January 2009, a change in Editorial staff occurred and these guidelines address the ongoing transition and update the online "Suggestions for Contributors" guidelines provided on the PNAT website (http://www.fhsu.edu/biology/pn/prairienat.htm); these instructions supersede all previous guidelines. Tables and appendices are included for common word expressions with superfluous wording, examples of correct format and style guidelines for tables accompanying manuscripts, guidance in properly preparing Research Articles and Notes, citing literature, and mandatory abbreviations for tables, figures and parenthetical expressions.

Forage News [2009-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at KCA: New Year – New Challenges
• 2008 Variety Test Reports
• KFGC - Supporting KY’s Forage Industry
• New Publication Addresses Key Forage/Livestock Issue
• Fertilizing Forage Crops in 2009
• KFGC Sponsored Project First in the State
• Upcoming Events

A Nonsense Mutation In A Cinnamyl Alcohol Dehydrogenase Gene Is Responsible For The Sorghum Brown Midrib6 Phenotype1[W][Oa], Scott E. Sattler, Aaron J. Saathoff, Eric J. Haas, Nathan A. Palmer, Deanna L. Funnell, Gautam Sarath, Jeffrey F. Pedersen

Department of Agronomy and Horticulture: Faculty Publications

Recently, bmr6 plants were shown to have limited cinnamyl alcohol dehydrogenase activity (CAD; EC 1.1.1.195), the enzyme that catalyzes the conversion of hydroxycinnamoyl aldehydes (monolignals) to monolignols. A candidate gene approach was taken to identify Bmr6. Two CAD genes (Sb02g024190 and Sb04g005950) were identified in the sorghum genome based on similarity to known CAD genes and through DNA sequencing a nonsense mutation was discovered in Sb04g005950 that results in a truncated protein lacking the NADPH-binding and C-terminal catalytic domains. Immunoblotting confirmed that the Bmr6 protein was absent in protein extracts from bmr6 plants. Phylogenetic analysis indicated that Bmr6 is …

Discovery: The Student Journal Of Dale Bumpers College Of Agricultural, Food And Life Sciences - Volume 10 2009, Several Authors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Skunk Cabbage Flowers: The Heat Is On, W. John Hayden

Biology Faculty Publications

It is a remarkable sight, as striking as it is unexpected: January skunk cabbages emerging from the frozen mires they call home, melting their way through snow and ice. It seems so improbable because in everyday experience plants exist at equilibrium with ambient temperatures. Leave an apple on the front seat of your car in summer, it gets hot; examine your tomato plants on that first frosty morning in autumn, and they are permeated with ice crystals. People, mammals and birds are inherently warm, and pretty much nothing else is. Except, of course, for the exceptions, of which skunk cabbage …

Effects Of Stratification, Warm Treatment, And Mechanical And Acid Scarification On The Emergence Of Yellow-Flowered Alfalfa (Medicago Sativa Subsp. Falcata) Seeds, Diane Narem

The Journal of Undergraduate Research

Yellow-flowered alfalfa (YFA) has great potential to improve rangelands in the Northern Plains. Greater than 99% of YFA seeds from soil were viable but less than 4% germinated under standard laboratory conditions. The objective was to determine if low germination was due to physical or physiological seed dormancy. The experiment utilized a random complete block design with two trials, eight replications per trial. Each replication consisted of ten treatments plus a control, four stratification treatments consisting of a combination of two temperatures (-5°C, -20°C) and two durations (48 hr, 72 hr), one warm treatment (4 min, 60°C), four sandpaper scarification …

Development Of A Quantitative Pasture Phosphorus Management Tool Using The Swat Model, Michael J. White, Daniel E. Storm, Michael D. Smolen, Hailin Zhang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Assessment tools to evaluate phosphorus loss from agricultural lands allow conservation planners to evaluate the impact of management decisions on water quality. Available tools to predict phosphorus loss from agricultural fields are either: (1) qualitative indices with limited applicability to address offsite water quality standards, or (2) models which are prohibitively complex for application by most conservation planners. The purpose of this research was to develop a simple interface for a comprehensive hydrologic ⁄ water quality model to allow its usage by farmers and conservation planners. The Pasture Phosphorus Management (PPM) Calculator was developed to predict average annual phosphorus (P) …

Phylogenetic Classification Of Escherichia Coli O157:H7 Strains Of Human And Bovine Origin Using A Novel Set Of Nucleotide Polymorphisms, Michael L. Clawson, James Keen, Timothy Smith, Lisa Durso, Tara G. Mcdaneld, Robert Mandrell, Margaret Davis, James Bono

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Cattle are a reservoir of Shiga toxin-producing Escherichia coli O157:H7 (STEC O157), and are known to harbor subtypes not typically found in clinically ill humans. Consequently, nucleotide polymorphisms previously discovered via strains originating from human outbreaks may be restricted in their ability to distinguish STEC O157 genetic subtypes present in cattle. The objectives of this study were firstly to identify nucleotide polymorphisms in a diverse sampling of human and bovine STEC O157 strains, secondly to classify strains of either bovine or human origin by polymorphism-derived genotypes, and finally to compare the genotype diversity with pulsed-field gel electrophoresis (PFGE), a …

Sorption Of Phosphorus From Swine, Dairy, And Poultry Manures, Carl H. Bolster, Karamat R. Sistani

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In most phosphorus (P) sorption studies, P is added as an inorganic salt to a predefined background solution such as calcium chloride (CaCl₂) or potassium chloride (KCl); however, in many regions, the application of P to agricultural fields is in the form of animal manure. The purpose of this study, therefore, was to compare the sorption behavior of dissolved reactive P (DRP) in monopotassium phosphate (KH₂PO₄)–amended CaCl₂ and KCl solutions with sorption behavior of DRP in three different animal manure extracts. Phosphorus single-point isotherms (PSI) were conducted on eight soils with the following …

Enzymatic Quantification Of Phytate In Animal Manure, Zhongqi He, Heidi W. Waldrip, C. Wayne Honeycutt, M. Susan Erich, Zachary N. Senwo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phytate (inositol hexaphosphate) has been identified as a major organic phosphorus (P) form in soil, animal manure, and other environmental samples. Although a number of methods are available for quantitative isolation and determination of phytate, they are time-consuming and not amenable to routine analysis. We developed a simple, rapid method for enzymatic determination of phytate in animal manure. Animal manure was extracted by H₂O, 1M hydrochloric acid (HCl), 0.1M sodium acetate (NaOAc, pH5.0) with or without 0.05M ethylenediaminetetraacetate (EDTA), and 0.25M or 0.5M sodium hydroxide (NaOH)–0.05M EDTA. Extracts were diluted (1/10–1/150) and adjusted to pH5.0 in sodium acetate …

Natural Enemies Of The Coccinellidae: Parasites, Pathogens, And Parasitoids, E. W. Riddick, T. E. Cottrell, K. A. Kidd

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We review aspects of the life histories of representative enemies of coccinellids (both entomophagous and phytophagous species) and expose both potential and real effects that they have on life parameters of their hosts. Lady beetles are attacked by a variety of natural enemies (bacteria, fungi, mites, nematodes, protozoa, wasps, flies). Few of these enemies have the ability to alter significantly the population dynamics of their hosts. This review should encourage further research to help define the role of natural enemies in the population dynamics of coccinellids. Ultimately, the conservation of beneficial lady beetles and the management of nuisance and pestiferous …

Should The Mirid, Eccritotarsus Catarinensis (Heteroptera: Miridae), Be Considered For Release Against Water Hyacinth In The United States Of America?, Julie A. Coetzee, Marcus J. Byrne, Martin P. Hill, Ted D. Center

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Between one and seven biological control agents have been released against water hyacinth (Eichhornia crassipes (Mart.) Solms) in at least 30 countries, with varied success. A mirid, Eccritotarsus catarinensis (Carvalho) (Heteroptera: Miridae), the most recent agent released, is damaging to the plant on the African continent.

The Rediscovery Of Some Taxa Thought To Have Been Extinct In Turkey, Ali̇ Kandemi̇r

Turkish Journal of Botany

This paper reports the re-discovery of Barbarea auriculata Hausskn. ex Bornm. var. auriculata (Brassicaceae), Onobrychis nitida Boiss. (Fabaceae), Onosma discedens Hausskn. ex Bornm. (Boraginaceae), and Silene oligotricha Hub.-Mor. (Caryophyllaceae). All of these taxa had been listed as Extinct (EX), according to the World Conservation Union Red List Categories. This study provides the re-descriptions of these taxa, presents field observations of these taxa, and suggests new IUCN categories.

The Role Of Polyethylene Glycol (Peg) Pretreatment In Improving Sugarcane’S Salt (Nacl) Tolerance, Neelma Munir, Faheem Aftab

Turkish Journal of Botany

An important aspect of salt tolerance studies in different plants is the relationship between salt and water stress. In the present study calluses (60 days old) of 2 sugarcane cultivars (cv. SPF 234 and cv. HSF 240) were treated with 4 different salt concentrations, including a control (0 mM NaCl), after 1% polyethylene glycol (PEG) pretreatment for 5 days. Fresh weight, callus browning, and necrosis data were recorded at day 120. Biochemical parameters (soluble protein contents, and peroxidase, catalase, and superoxide dismutase activity) were also analysed. Regardless of PEG pretreatment, calluses subjected to salt stress had less fresh weight than …

Chamaespartium Adans. (Leguminosae): A New Record For The Flora Of Turkey, Asuman Efe, Engi̇n Özhatay, Necmi̇ Aksoy, Di̇lek Demi̇r Oral

Turkish Journal of Botany

Chamaespartium sagittale (L.) P.E.Gibbs (Leguminosae) is a new record for the flora of Turkey with specimens collected from Yuvacık Dam (Kocaeli province, NW Turkey). Its description, distribution maps, and photos are given.

7tmrmine: A Web Server For Hierarchical Mining Of 7tmr Proteins, Guoqing Lu, Zhifang Wang, Alan M. Jones, Etsuko N. Moriyama

Center for Plant Science Innovation: Faculty Publications

Background: Seven-transmembrane region-containing receptors (7TMRs) play central roles in eukaryotic signal transduction. Due to their biomedical importance, thorough mining of 7TMRs from diverse genomes has been an active target of bioinformatics and pharmacogenomics research. The need for new and accurate 7TMR/GPCR prediction tools is paramount with the accelerated rate of acquisition of diverse sequence information. Currently available and often used protein classification methods (e.g., profile hidden Markov Models) are highly accurate for identifying their membership information among already known 7TMR subfamilies. However, these alignment-based methods are less effective for identifying remote similarities, e.g., identifying proteins from highly divergent or possibly …

2009 Spring Seed Book, Nebraska Crop Improvement Association

Nebraska Crop Improvement Association Seed Guides

FOUNDATION SEED

PLANT VARIETY PROTECTION ACT and HOW IT BENEFITS YOU!

TURFGRASSES

PERENNIAL FORAGE GRASSES

SPRING BARLEY

SPRING WHEAT

OAT VARIETY CHARACTERISTICS - 2009

OATS

MILLET

MILLET VARIETY CHARACTERISTICS 2009

HYBRID SEED CORN

NEBRASKA SEED QUALITY ASSURANCE® PROGRAM

SOYBEANS BUYERS’ NOTICE

APPROVED SEED CONDITIONERS

CUSTOM CERTIFIED CONDITIONERS

2008 NCIA MEMBERS

Instructions For Authors, Discovery Editors

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Letter From The Dean, Lalit Verma

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

No abstract provided.

Research Emphasis – Forage And Bioenergy Crop Management, John A. Guretzky

Department of Agronomy and Horticulture: Faculty Publications

Guretzky specializes in research on forage and bioenergy crop management that will assist agricultural producers and landowners in meeting their goals. The first area of research involves Drs. Jon Biermacher and Billy Cook for the evaluation of management practices of switchgrass in forage and bioenergy systems. Objectives include: 1.) assessing potential on-farm biomass yields and production economics of switchgrass; 2.) documenting effects of location, harvest system and nitrogen fertilizer rates on biomass yields, forage quality, stand persistence and nutrient removal rates; 3.) evaluating establishment and biomass production responses to phosphorus and potassium fertilization; 4.) determining how stocker cattle gains on …

Variation Of Morphology And Elemental Concentrations In The California Nickel Hyperaccumulator Streptanthus Polygaloides (Brassicaceae), Robert S. Boyd, Michael A. Wall, Scott R. Santos, Michael A. Davis

Faculty Publications

The Ni hyperaccumulator Strepthanthus polygaloides (Brassicaceae) is one of a handful of Ni hyperaccumulators known from continental North America. Surveys have revealed four distinctive morphs of this species, relying primarily on floral traits (sepal color and shape): a purple sepal morph (P), a yellow sepal morph (Y), a morph in which sepals start yellow and mature to purple (Y/P), and a morph with light yellow undulate sepals (U). In this study, we raised plants from ten populations (five Y, three P, one Y/P, and one U) under uniform greenhouse conditions to determine if morphs varied in morphology and elemental concentrations …

Evaluating The Effects Of Organic And Conventional Inputs On Soil Chemical And Biological Properties In A Four-Year Vegetable Rotation And The Investigation Of Soil Microbial Properties On Plant Gene Expression, Audrey Law

University of Kentucky Doctoral Dissertations

The objective of this research was to determine the effects of conventional inputs on soil chemical and biological properties compared to organic systems in a four year vegetable rotation. Tillage and cover crops were the same in all treatments to avoid confounding factors often present in similar research. Additional experiments investigated plant gene expression in organic and conventional management systems and in soils with decreased microbial diversity. Experimental plots were prepared in the spring of 2004; four replications of three management treatments, organic, low-input and conventional, were arranged in a randomized complete block design. The rotation consisted of edamame soybean, …

Sfa Gardens Scrapbook 2005-2009, Sfa Gardens, Stephen F. Austin State University

SFA Gardens Scrapbooks

No abstract provided.