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Evaluation Of Rice Stink Bug, Oebalus Pugnax (F.), Damage And Monitoring Techniques In Rice, Oryza Sativa L., And Grain Sorghum, Sorghum Bicolor (L.), Aaron Joseph Cato

Graduate Theses and Dissertations

Rice stink bug, Oebalus pugnax (F.), is a serious pest of headed rice, Oryza sativa L. and an occasional pest of heading grain sorghum in the Mid-south. Work from this dissertation focuses on resolving gaps in and knowledge of rice stink bug sampling and management, and attempts to create a basis for rice stink bug damage assessment in future studies.

Field experiments were conducted from 2016-2018 to asses variation in sweep net sampling by observing producers, researchers, extension personnel, consultants and their workers. Large levels of variation were found in sweep lengths between observed sweepers and reliability of smaller sweep …

Efficacy Of Antimicrobial Mitigation On Escherichia Coli Cfu And Growth And Development Of Hydroponic Leafy Greens, Nathan J. Eylands

Graduate Theses and Dissertations

The Food and Drug Administration (FDA) have set new standards that apply to agriculturalists producing crops eaten fresh and/or raw by consumers. This new produce safety rule, known as the Food Safety Modernization Act (FSMA), has established science-based standards for all areas of production in agriculture with regards to microbial contamination. Escherichia coli (E. coli) O157:H7 is a particular bacterium of concern under FSMA guidelines. Grower compliance is mandatory and therefore vital to the continuation of any farm. Greenhouse hydroponic growers have shown advantages in efficiency when compared to conventional farming methodology. Those, however, with recirculating hydroponic systems face unique …

Impact Of Roadside Maintenance Practices On Larinus Minutus (Gyllenhal), A Biological Control Agent Of Spotted Knapweed, Mary Elizabeth Ferguson

Graduate Theses and Dissertations

Spotted knapweed, Centaurea stoebe, is an invasive weed found throughout much of the United States. Spotted knapweed is a rangeland weed where it was originally introduced into western North America in the 1880s.Where spotted knapweed spread to the southeastern U.S., it is found mostly along roadsides. It has been the focus of a biological control program beginning in the 1960s, with 12 insects established, with the final introductions occurring in the 1990s. After the success observed in the western U.S. and Canada with one of these insects, Larinus minutus, this weevil was established in northwestern Arkansas. It is too early …

Epidemiology And Management Of Fusarium Head Blight And Foliar Fungal Diseases Of Wheat, Carlos Bolanos-Carriel

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fusarium head blight (FHB) caused by Fusarium graminearum, the FHB-associated mycotoxin deoxynivalenol (DON), and foliar fungal diseases are significant threats to wheat production. This research 1) evaluated the effects of fungicide chemical class, application timing, and cultivar resistance on FHB and DON under field conditions; 2) evaluated the effects of field-applied fungicide chemical class, grain moisture, and time on DON under grain storage conditions; 3) evaluated the effects of field-applied fungicide chemical class and time on trichothecene-related gene (Tri5) expression under grain storage conditions; 4) determined the optimum F. graminearum spore concentration and spike bagging period following …

Repeat Elements Organise 3d Genome Structure And Mediate Transcription In The Filamentous Fungus Epichloë Festucae, David J. Winter, Austen R. D. Ganley, Carolyn A. Young, Ivan Liachko, Christopher L. Schardl, Pierre-Yves Dupont, Daniel Berry, Arvina Ram, Barry Scott, Murray P. Cox

Plant Pathology Faculty Publications

Structural features of genomes, including the three-dimensional arrangement of DNA in the nucleus, are increasingly seen as key contributors to the regulation of gene expression. However, studies on how genome structure and nuclear organisation influence transcription have so far been limited to a handful of model species. This narrow focus limits our ability to draw general conclusions about the ways in which three-dimensional structures are encoded, and to integrate information from three-dimensional data to address a broader gamut of biological questions. Here, we generate a complete and gapless genome sequence for the filamentous fungus, Epichloë festucae. We use Hi-C …

Evaluation Of Biological Agents For Control Of Macrophomina Root Rot And Powdery Mildew In Flowering Dogwood (Cornus Florida L.), Margaret T. Mmbaga, Lucas M. Mackasmiel, Frank A. Mrema

Agricultural and Environmental Sciences Faculty Research

Six biological control agents (BCAs) (two bacteria, two fungi, and two yeasts) that were previously shown to be effective against powdery mildew (Erysiphe pulchra) were tested for efficacy against Macrophomina phaseolina root rot on flowering dogwood (Cornus florida) in the greenhouse. Two of the bacterial isolates, Stenotrophomonas sp. (B17A) and Serratia sp. (B17B), were effective in controlling both macrophomina root rot and powdery mildew, similar to fungicide control thiophanate methyl, when roots were drenched with the six BCAs individually. In addition, the two bacterial BCAs improved plant growth with respect to stem diameter, stem length, dry weight, and green foliage …

An Inquiry-Based Investigation Of Bacterial Soft Rot Of Potato, Robert Louis Hirsch, Seth Miller, Dennis Halterman

Plant Pathology Faculty Publications

Inquiry-based investigations of diseases are often difficult to safely undertake in middle school or high school science courses. However, by utilizing potatoes as a mammalian analogue, important groups of pathogens can be investigated with common materials available from the local supermarket. This article provides information to guide the exploration of factors underlying the development of the potato disease bacterial soft rot, caused by Pectobacterium caratovorum, and allows students the freedom to develop and test their own hypotheses regarding the development of symptoms, the spread of pathogens, and the impact of host and environmental variables on the progress of disease.

Capsid Structure Of Dsrna Fungal Viruses, Daniel Luque, Carlos P. Mata, Nobuhiro Suzuki, Said A. Ghabrial, José R. Castón

Plant Pathology Faculty Publications

Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix …

Dna Barcoding Evidence For The North American Presence Of Alfalfa Cyst Nematode, Heterodera Medicaginis, Tom Powers, Andrea Skantar, Timothy Harris, Rebecca Higgins, Peter Mullin, Saad Hafez, Zafar Handoo, Tim Todd, Kirsten S. Powers

Department of Plant Pathology: Faculty Publications

Specimens of Heterodera have been collected from alfalfa fields in Kearny County, Kansas and Carbon County, Montana. DNA barcoding with the COI mitochondrial gene indicate that the species is not Heterodera glycines, soybean cyst nematode, H. schachtii, sugar beet cyst nematode, or H. trifolii, clover cyst nematode. Maximum likelihood phylogenetic trees show that the alfalfa specimens form a sister clade most closely related to H. glycines, with a 4.7% mean pairwise sequence divergence across the 862 nucleotides of the COI marker. Morphological analyses of juveniles and cysts conform to the measurements of H. medicaginis, the alfalfa cyst nematode originally described …

Grapevine Vein Clearing Virus: Epidemiological Patterns And Construction Of A Clone, Cory Von Keith

Graduate Theses/Dissertations

Grapevine vein clearing virus (GVCV) is a recently discovered virus belonging to the Badnavirus genus. Characteristic to its name, the virus is associated with a disease where symptoms manifest as pronounced vein-clearing, resulting in severe berry deformation and vine decline in susceptible grape varieties. Sustainable production of wine is dependent on healthy plants. The associated disease is mainly found in Midwest vineyards. Attempts were made in this thesis to provide evidence of causality of the virus to the associated disease and to infer the historical path and migration pattern of GVCV. Conclusions and discussions will provide grape producers with the …

Pipecolic Acid Confers Systemic Immunity By Regulating Free Radicals, Caixia Wang, Ruiying Liu, Gah-Hyun Lim, Laura De Lorenzo, Keshun Yu, Kai Zhang, Arthur G. Hunt, Aardra Kachroo, Pradeep Kachroo

Plant Pathology Faculty Publications

Pipecolic acid (Pip), a non-proteinaceous product of lysine catabolism, is an important regulator of immunity in plants and humans alike. In plants, Pip accumulates upon pathogen infection and has been associated with systemic acquired resistance (SAR). However, the molecular mechanisms underlying Pip-mediated signaling and its relationship to other known SAR inducers remain unknown. We show that in plants, Pip confers SAR by increasing levels of the free radicals, nitric oxide (NO), and reactive oxygen species (ROS), which act upstream of glycerol-3-phosphate (G3P). Plants defective in NO, ROS, G3P, or salicylic acid (SA) biosynthesis accumulate reduced Pip in their distal uninfected …

Assembly-Hub Function Of Er-Localized Snare Proteins In Biogenesis Of Tombusvirus Replication Compartment, Zsuzsanna Sasvari, Nikolay Kovalev, Paulina Alatriste Gonzalez, Kai Xu, Peter D. Nagy

Plant Pathology Faculty Publications

Positive-strand RNA viruses assemble numerous membrane-bound viral replicase complexes within large replication compartments to support their replication in infected cells. Yet the detailed mechanism of how given subcellular compartments are subverted by viruses is incompletely understood. Although, Tomato bushy stunt virus (TBSV) uses peroxisomal membranes for replication, in this paper, we show evidence that the ER-resident SNARE (soluble NSF attachment protein receptor) proteins play critical roles in the formation of active replicase complexes in yeast model host and in plants. Depletion of the syntaxin 18-like Ufe1 and Use1, which are components of the ER SNARE complex in the ERAS (ER …

Evaluation Of Bacillus Strains For Plant Growth-Promotion Potentials On Corn (Zea Mays), Wheat (Triticum Aestivum), And Soybean (Glycine Max)., Rufus John Akinrinlola

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Plant growth-promoting rhizobacteria (PGPR) can increase plant growth and yield by facilitating nutrient availability, hormone production, and inhibiting plant deleterious microorganisms. Twelve strains of bacillus (endospore-forming bacteria belonging to the genera Bacillus, Paenibacillus and Lysinibacillus) isolated from wheat rhizospheres were assessed for plant-growth promotion attributes in greenhouse and laboratory experiments. The objectives were to assess each strain’s potential to promote growth in corn, wheat, and soybean; and to determine whether the physiological traits expressed in vitro by the strains related to their effectiveness in promoting plant growth. Greenhouse experiments to assess growth-promotion potential were conducted by applying the strains …

Seasonal Phenology, Distribution And Treatments For Polyphagotarsonemus Latus (Banks) On Primocane-Fruiting Blackberries (Rubus L. Subgenus Rubus) In Arkansas, Jessica Anne Lefors

Graduate Theses and Dissertations

Worldwide, blackberries (Rubus L. subgenus Rubus) are an economically important crop. In 2007, Polyphagotarsonemus latus (Banks) (broad mites), were first reported damaging primocane-fruiting blackberries in Fayetteville, Arkansas. Since this time, broad mite damage to blackberries and yield loss has been reported in many states and countries. Despite the increasing reports of this blackberry pest, little is known about their population dynamics, and few treatments are available. Growers with broad mite populations need a pest management program to minimize yield loss. Therefore, there was a need to develop sampling techniques, describe broad mite seasonal phenology in blackberry fields, and determine efficacy …

Assessment Of Resistance To Botrytis Cinerea In Arabidopsis Expressing Grapevine Sts Genes And Analysis Of New Grapevine Vein Clearing Virus Isolates, Li Su

Graduate Theses/Dissertations

Grapevine stilbene synthase gene (STS) family is unusually large with more than 32 members of full-length genes. Resveratrol and piceid are synthesized in VaSTS7-, VvSTS22-, VaSTS22-transgenic Arabidopsis. To study the function of these STS genes in the resistance against Botrytis cinerea in vivo, lesion sizes on these STS transgenic Arabidopsis were compared to that on non-transgenic control Col-0, at 96 hours post inoculation with B. cinerea spores at 0, 200, or 1,000 spores/μl. In each experiment there were three biological replicates. Three lesion sizes from each replicate were averaged. The experiment was repeated three …

Combining Translational And Functional Genomic Approaches To Augment Management Strategies Of Plant Parasitic Nematodes, Sandeep Sharma Khatiwada

Graduate Theses and Dissertations

Plant-parasitic nematodes pose a major threat to crop yield worldwide. Discontinued use of harmful chemicals has prompted the search for alternative management strategies that are effective yet environmentally friendly. Harpin proteins, which are derived from bacteria, and nematophagous fungi, natural predators of nematodes, are ideal for biological control of plant-parasitic nematodes. However, research on the efficacy of harpin proteins on nematodes, and biology of nematophagous fungi is minimal. Previously, a taxonomically uncharacterized nematophagous fungus designated ARF18 effectively suppressed nematode populations in soil. The overarching goal of this dissertation is to utilize applied and functional genomic approaches to augment management of …

Evaluation Of Dicamba Off-Target Movement And Subsequent Effects On Soybean Offspring, Gordon Travis Jones

Graduate Theses and Dissertations

Commercial launch of cotton with resistance to dicamba, glyphosate, and glufosinate occurred in 2015 and launch of soybean with resistance to dicamba and glyphosate occurred in early 2016. It is likely that non-dicamba-resistant soybean will be planted in close proximity to dicamba-resistant soybean and cotton. Therefore, experiments were conducted to examine the distance dicamba moves during an application using commercial application equipment, as well as the effect the drift events have upon soybean offspring. Additional experiments were designed to investigate the effect glyphosate addition to dicamba has upon soybean growth and yield as well as possible effects on offspring. Lastly, …

Genetic And Phytopathological Studies On Charcoal Rot Resistance In Soybean [Glycine Max (L) Merr.], Marcos Paulo Da Silva

Graduate Theses and Dissertations

Charcoal rot of soybean (Glycine max (L.), caused by Macrophomina phaseolina is a disease of economic significance in the United States. The identification and quantification of the resistance is difficult, and very little is known about the genetics and markers linked to the charcoal rot (CR) resistance genes. Current assay methods can be time consuming, and data may vary between tests. The objectives of this study were to 1) create a robust seed plate assay (SPA) for CR resistance by comparing results with cut-stem and CFUI assays; 2) correlate and compare field data disease assessments with SPA; 3) identify QTLs …

A Genome Wide Association Study For Fusarium Head Blight Resistance In Southern Soft Red Winter Wheat, Amanda Holder

Graduate Theses and Dissertations

Fusarium head blight (FHB) is a disease of small grains caused by the fungal pathogen Fusarium graminearum. FHB poses potential economic losses and health risks due to the accumulation of the mycotoxin deoxynivalenol (DON) on infected seed heads. The objectives of this study are: 1) evaluate soft red winter wheat (SRWW) lines for resistance to FHB in terms of resistance to initial inoculum (incidence); resistance to spread within the head (severity); resistance to DON accumulation; and resistance to Fusarium damaged kernels (FDK), 2) determine the frequency and effect of known FHB resistance genes and quantitative trait loci (QTL), and 3) …

Clarireedia: A New Fungal Genus Comprising Four Pathogenic Species Responsible For Dollar Spot Disease Of Turfgrass, Catalina Salgado-Salazar, Lisa A. Beirn, Adnan Ismaiel, Michael J. Boehm, Ignazio Carbone, Alexander I. Putman, Lane P. Tredway, Bruce B. Clarke, Jo Anne Crouch

Department of Plant Pathology: Faculty Publications

Dollar spot is one of the most destructive and economically important fungal diseases of amenity turfgrasses. The causal agent was first described in 1937 as the ascomycete Sclerotinia homoeocarpa. However, the genus-level taxonomic placement of this fungus has been the subject of an ongoing debate for over 75 y. Existing morphological and rDNA sequence evidence indicates that this organism is more appropriately placed in the family Rutstroemiaceae rather than the Sclerotiniaceae. Here we use DNA sequence data from samples of the dollar spot fungus and other members of the Rutstroemiaceae (e.g. Rutstroemia, Lanzia, Lambertella) collected throughout the world to determine …

Exploration Of Soybean Nodule Microbiome For Plant Health Management, Serkan Tokgöz

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Root nodule is a unique environment formed in the legume roots through a highly specific symbiotic relationship between leguminous plants and nodule inducing bacteria. Over the years, Rhizobia have been thought as the only group of bacteria residing within the nodules. However, this consideration has been recently changed with the discovery of other groups of bacteria besides Rhizobia within the legume nodules. In an effort to identify beneficial bacteria for plant disease control and growth promotion, soybean nodules were studied as the source of nodule-associated bacteria. Metagenomics analysis of a single soybean nodule was conducted to determine the bacterial diversity …

Does The Fungal Pathogen (Botryosphaeria Dothidea) Exceed The Dehydration Tolerance Of Its Chaparral Host?, Cristian M. Garcia

Seaver College Research And Scholarly Achievement Symposium

We tested the hypothesis that an opportunistic endophytic fungus Botryosphaeria dothidea that frequently infects and causes dieback in several different species of chaparral shrubs in the Santa Monica Mountains continues to elongate and grow in host tissues at dehydration levels that exceed host survival. This was done by collecting several large branches from the field from three dominant species of co-occurring chaparral shrubs, Malosma laurina, Ceanothus spinosus, and Ceanothus megacarpus. We allowed branches to dehydrate at increasing lengths of time, from a few days to one week, in an air-conditioned laboratory, then sealed in plastic bags to allow tissuewater equilibration, …

Breeding For Resistance In California Strawberry To Verticillium Dahliae, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This article focuses on breeding resistant strawberries to the fungus Verticillium dahliae, common name Verticillium wilt. This is a serious fungal disease that can result in a loss of 50% or more of a strawberry harvest when grown in infested soil. The main goal is to provide an example of how a cultivar can be made more resistant to a plant pathogen with the use of plant breeding methods.

Since 1930 the University of California, Davis, has been developing strawberry cultivars that are adapted to the agricultural industry and regional farms. Developing cultivars that require fewer inputs are of …

2018 Barley Variety Fact Sheets For Western Australia, Blakely Paynter, Georgia Trainor, Raj Malik, Jeremy Curry, Sanjiv Gupta, Sarah Collins, Daniel Huberli, Harmohinder Dhammu, Geoff Thomas, Ben Biddulph

Bulletins 4000 -

This bulletin is designed as a reference to help determine which barley variety to grow in your region. It provides market feedback, relative grain yield comparisons, disease ratings, agronomic information and herbicide tolerance ratings for all malt barley varieties segregated in Western Australia (WA) and selected feed varieties.

The decision whether to grow barley with a malt or feed classification depends on five main factors:

(1) Premium paid for different varieties when segregated.

(2) Relative grain yield of malt and feed grade barley varieties.

(3) Differences in input costs due to their agronomic and disease characteristics.

(4) Likelihood that grain …

Proceedings Of The 45th Annual Meeting Of The Southern Soybean Disease Workers (March 7-8, 2018, Pensacola Beach, Florida), Heather M. Kelly, Terry Spurlock, Carl Bradley, Danise Beadle, Patricia Bollich, Trey Price, Tom Allen

Southern Soybean Disease Workers: Conference Proceedings

Contents

List of SSDW Officers

Agenda for March 7-8, 2018

Abstracts for contributed/presented papers

Retrospective analyses regarding the impact of soybean diseases in the USA .TW Allen, PD Esker, and CA Bradley

Diaporthe (Phomopsis) species on soybean: current status in the United States. Febina Mathew, Kristina Petrovic, Lisa Castlebury, Tom Allen, Gary Bergstrom, John Bonkowski, Carl Bradley, James Buck, Emmanuel Byamukama, Martin Chilvers, Anne Dorrance, Nicholas Dufault, Loren Giesler, Nancy Gregory, Heather Kelly, Nathan Kleczewski, Trey Price, Dean Malvick, Sam Markell, Daren Mueller, Damon Smith, Terry Spurlock, Kiersten Wise, and Marina Johnson

New insights into the genetic underpinnings …

Characterization Of Botrytis Cinerea Resistance To Fungicides In California Strawberry Production, Scott D. Cosseboom

Master's Theses

Gray mold of strawberry, caused by Botrytis cinerea, is a very destructive pre- and post-harvest fruit rot. Outside of California, fungicide resistance in B. cinerea has been reported to every site-specific chemical class labeled for use against gray mold. One objective of this study was to characterize the resistance of 888 isolates of B. cinerea from California strawberry fields to ten active ingredients. Isolates were collected from the same planting block in 47 fields during the early-season (0 to 8 fungicide applications) and late-season (16 to 26 fungicide applications) of 2016. Sensitivity of each isolate was determined using the …

Mac3a And Mac3b, Two Core Subunits Of The Mos4-Associated Complex, Positively Influence Mirna Biogenesis, Shengjun Li, Kan Liu, Bangjun Zhou, Mu Li, Shuxin Zhang, Lirong Zeng, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MAC3A and MAC3B are conserved U-box containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis. However, their functional mechanisms remain elusive. Here we show that Arabidopsis thaliana MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs ( …

A New Toolset For Protein Expression And Subcellular Localization Studies In Citrus And Its Application To Citrus Tristeza Virus Proteins, Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael M. Goodin, Vladimir Orbovic

Plant Pathology Faculty Publications

Background

Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium.

Results

Here we describe an improved method for gene expression in epidermal cells of citrus leaves, using the Bio-Rad Helios gene-gun. …

Evaluation Of Bacillus Strains For Plant Growth Promotion And Predictability Of Efficacy By In Vitro Physiological Traits, Rufus J. Akinrinlola, Gary Y. Yuen, Rhae A. Drijber, Anthony O. Adesemoye

Department of Plant Pathology: Faculty Publications

Bacilli are commonly used as plant growth-promoting agents but can be limited in effectiveness to certain crop and soil environments. The objectives of this study were to (1) identify Bacillus strains that can be consistent in promoting the growth of corn, wheat, and soybean and (2) determine whether physiological traits expressed in vitro can be predictive of growth promotion efficacy/consistency and be used for selecting effective strains. Twelve Bacillus strains isolated from wheat rhizospheres were evaluated in greenhouse pot tests with nonsterile soil for their effects on the growth of corn, soybean, and wheat. The strains also were assessed in …

Biophysical Approaches To Solve The Structures Of The Complex Glycan Shield Of Chloroviruses, Cristina De Castro, Garry Duncan, Domenico Garozzo, Antonio Molinaro, Luisa Sturiale, Michela Tonetti, James L. Van Etten

James Van Etten Publications

The capsid of Paramecium bursaria chlorella virus (PBCV-1) contains a heavily glycosylated major capsid protein, Vp54. The capsid protein contains four glycans, each N-linked to Asn. The glycan structures are unusual in many aspects: (1) they are attached by a β-glucose linkage, which is rare in nature; (2) they are highly branched and consist of 8–10 neutral monosaccharides; (3) all four glycoforms contain a dimethylated rhamnose as the capping residue of the main chain, a hyper-branched fucose residue and two rhamnose residues ''with opposite absolute configurations; (4) the four glycoforms differ by the nonstoichiometric presence of two monosaccharides, l-arabinose and …