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Corn Disease Update, Tamra A. Jackson-Ziems

Department of Plant Pathology: Faculty Publications

The harsh growing conditions during 2014 contributed to several disease problems in corn, in spite of the overall high yields across the state. The extended cold and wet conditions early in the season led to development of seedling diseases and crop stress. Then, in many parts of the state, repeated and unprecedented strong storms battered and wounded crops with multiple and widespread hail events, high winds, very heavy rain, and devastating tornadoes. Many fields were planted late or replanted, some more than once, further delaying the crop. Cool summer and fall conditions delayed maturity and led to early frost events …

What’S New In Plant Pathology, Tamra A. Jackson-Ziems, Loren Giesler, Robert M. Harveson, Stephen N. Wegulo, Kevin A. Korus, Tony O. Adesemoye

Department of Plant Pathology: Faculty Publications

Extension Plant Pathology Team Update

Changes to the Plant Disease Management Section of the 2015 Weed Guide

During the past year, several new products have become available for disease management. These products and additional products have been added to the Plant Disease Management Section of the 2015 Weed Guide. Products added to the Weed Guide have been summarized in Tables 1-3.

Table 1. Foliar Products

Table 2. Seed Treatment Products

Table 3. Seed Treatment Nematicides

Differentiating Chemical And Disease Symptoms In The Field, Kevin A. Korus, Loren J. Giesler, Robert M. Harveson, Tamra A. Jackson-Ziems, Lowell Sandell, Stephen Wegulo

Department of Plant Pathology: Faculty Publications

In years with conditions favorable for seedling disease and other disease there are typically concerns and inquiries about the cause(s) of symptoms in agronomic plants. Many of the questions focus on differentiating between plant injuries potentially caused by recent herbicide applications versus symptoms caused by plant pathogens. Chemical injury in row crops is caused by chemicals such as herbicides, fertilizers, fungicides, insecticides, growth regulators, and crop oils when they are applied individually, as a mixture, or together with adjuvants. If chemicals are applied incorrectly, plants may be damaged at any point during the growing season. Chemical injury can result from …

Large Dsdna Chloroviruses Encode Diverse Membrane Transport Proteins, Gerhard Thiel, Timo Greiner, David Dunigan, Anna Moroni

James Van Etten Publications

Many large DNA viruses that infect certain isolates of chlorella-like green algae (chloroviruses) are unusual because they often encode a diverse set of membrane transport proteins, including functional K+ channels and aquaglyceroporins as well as K+ transporters and calcium transporting ATPases. Some chloroviruses also encode putative ligand-gated-like channel proteins. No one protein is present in all of the chloroviruses that have been sequenced, but the K+ channel is the most common as only two chloroviruses have been isolated that lack this complete protein. This review describes the properties of these membrane-transporting proteins and suggests possible physiological functions and evolutionary histories …

Effect Of Waxy (Low Amylose) On Fungal Infection Of Sorghum Grain, Deanna L. Funnell-Harris, Scott E. Sattler, Patrick M. O'Neill, Kent M. Eskridge, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Loss of function mutations in waxy, encoding granule bound starch synthase (GBSS) that synthesizes amylose, results in starch granules containing mostly amylopectin. Low amylose grain with altered starch properties has increased usability for feed, food, and grain-based ethanol. In sorghum, two classes of waxy (wx) alleles had been characterized for absence or presence of GBSS: wx^a (GBSS^-) and wx^b (GBSS+, with reduced activity). Field-grown grain of wild-type; waxy, GBSS^-; and waxy, GBSS⁺ plant introduction accessions were screened for fungal infection. Overall, results showed that waxy grains were not more susceptible than …

Multi-Year Pathogen Survey Of Biofuel Switchgrass Breeding Plots Reveals High Prevalence Of Infections By Panicum Mosaic Virus And Its Satellite Virus, Catherine L. Stewart, Jesse D. Pyle, Charlene C. Jochum, Kenneth P. Vogel, Gary Y. Yuen, Karen-Beth G. Scholthof

Department of Plant Pathology: Faculty Publications

Switchgrass (Panicum virgatum) cultivars are currently under development as lignocellulosic feedstock. Here we present a survey of three established switchgrass experimental nurseries in Nebraska in which we identified Panicum mosaic virus (PMV) as the most prevalent virus. In 2012, 72% of 139 symptomatic plants tested positive for PMV. Of the PMV-positive samples, 19% were coinfected with its satellite virus (SPMV). Less than 14% of all sampled plants in 2012 were positive for four additional viruses known to infect switchgrass. In 2013, randomized sampling of switchgrass individuals from the same 2012 breeding plots revealed that infection by PMV or PMV+SPMV …

In Vitro Transcripts Of Wild-Type And Fluorescent Protein-Tagged Triticum Mosaic Virus (Family Potyviridae) Are Biologically Active In Wheat, Satyanarayana Tatineni, Anthony J. Mcmechan, Melissa Bartels, Gary L. Hein, Robert A. Graybosch

Department of Plant Pathology: Faculty Publications

Triticum mosaic virus (TriMV) (genus Poacevirus, family Potyviridae) is a recently described eriophyid mite-transmitted wheat virus. In vitro RNA transcripts generated from full-length cDNA clones of TriMV proved infectious on wheat. Wheat seedlings inoculated with in vitro transcripts elicited mosaic and mottling symptoms similar to the wildtype virus, and the progeny virus was efficiently transmitted by wheat curl mites, indicating that the cloned virus retained pathogenicity, movement, and wheat curl mite transmission characteristics. A series of TriMV-based expression vectors was constructed by engineering a green fluorescent protein (GFP) or red fluorescent protein (RFP) open reading frame with homologous …

Fine-Scale Genetic Structure Of Monilinia Fructicola During Brown Rot Epidemics Within Individual Peach Tree Canopies, Sydney E. Everhart, H. Scherm

Department of Plant Pathology: Faculty Publications

The purpose of this study was to determine the fine-scale genetic structure of populations of the brown rot pathogen Monilinia fructicola within individual peach tree canopies to better understand within-tree plant pathogen diversity and to complement previous work on spatiotemporal development of brown rot disease at the canopy level. Across 3 years in a total of six trees, we monitored disease development, collected isolates from every M. fructicola symptom during the course of the season, and created high-resolution three-dimensional maps of all symptom and isolate locations within individual canopies using an electromagnetic digitizer. Each canopy population (65 to 173 isolates …

Recommendations Of Generic Names In Diaporthales Competing For Protection Or Use, Amy Y. Rossman, Gerard C. Adams, Paul F. Cannon, Lisa A. Castlebury, Pedro W. Crous, Marieka Gryzenhout, Walter M. Jaklitsch, Luis C. Mejia, Dmitar Stoykov, Dhanushka Udayanga, Hermann Voglmayr, Donald M. Walker

Department of Plant Pathology: Faculty Publications

In advancing to one name for fungi, this paper treats generic names competing for use in the order Diaporthales (Ascomycota, Sordariomycetes) and makes a recommendation for the use or protection of one generic name among synonymous names that may be either sexually or asexually typified. A table is presented that summarizes these recommendations. Among the genera most commonly encountered in this order, Cytospora is recommended over Valsa and Diaporthe over Phomopsis. New combinations are introduced for the oldest epithet of important species in the recommended genus. These include Amphiporthe tiliae, Coryneum lanciforme, Cytospora brevispora, C. ceratosperma, C. …

Species Associated With Cytospora Canker On Populus Tremuloides, Jeff B. Kepley, F. Brent Reeves, William R. Jacobi, Gerard C. Adams

Department of Plant Pathology: Faculty Publications

A new species that is often associated with Cytospora chrysosperma was found commonly on stems of Populus tremuloides in Colorado. This fungus is illustrated and described as Cytospora notastroma sp. nov., and morphological and molecular data demonstrate that the new species is distinct from C. chrysosperma, C. nivea, C. translucens, and other species found on Populus. Diagnostic features are superficially visible, darkly pigmented conceptacles circumscribing the ascostromata and conidiomata and surrounding the white to grayish-white discs; some isolates produce a Phialocephala-like synanamorph in vitro in addition to the Cytospora anamorph.

Draft Genome Sequence Of Cercospora Arachidicola, Causal Agent Of Early Leaf Spot In Peanuts, Valerie A. Orner, Emily G. Cantowine, Xinye Monica Wang, Amr Abouelleil, James Bochicchio, Chad Nusbaum, Albert C. Culbreath, Zaid Abdo, Renee S. Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Cercospora arachidicola, causal agent of early leaf spot, is an economically important peanut pathogen. Lack of genetic information about this fungus prevents understanding the role that potentially diverse genotypes may have in peanut breeding programs. Here, we report for the first time a draft genome sequence of C. arachidicola.

The Taurine Biosynthetic Pathway Of Microalgae, Rahul Tevatia, James Allen, Deepak Rudrappa, Derrick White, Thomas E. Clemente, Heriberto Cerutti, Yaşar Demirel, Paul H. Blum

Center for Plant Science Innovation: Faculty Publications

Taurine (2-aminoethanesulfonic acid) is an amino acid–like compound widely distributed in animals and an essential nutrient in some species. Targeted metabolomics of marine and freshwater microalgae combined with medium supplementation identified biosynthetic pathway intermediates and necessary catalytic activities. Genomic analysis was then used to predict the first taurine biosynthetic pathway in these organisms. MRM-based electrospray ionization (ESI) LC–MS/MS analysis demonstrated that taurine is synthesized using a carbon backbone from L-serine combined with sulfur derived from sulfate. Metabolite analysis showed a nonuniform pattern in levels of pathway intermediates that were both species and supplement dependent. While increased culture salinity raised taurine …

Nitrogen Management Of Forages In Relation To Gaseous Emissions – New Approaches And Considerations, Shabtai Bittman, Derek Hunt

IGC Proceedings (1977-2023)

Forages have a high N demand, a long growing season, and an effective root system all contributing to effective nutrient capture. However forages are restrictive in methods available for mitigating gaseous losses both as NH₃ and as N₂O, due to both practical and cost considerations. Strategies are needed to address the challenges of both N efficiency and N losses. Agronomic techniques in long term experiments can enhance estimates of N loss pathways and N efficiency, and demonstrate the importance of integrated multinutrient approaches. The dual manure stream concept divides manure into a thin fraction suitable as an …

Impact Of Market Forces On Product Quality And Grassland Condition, David L. Michalk, Jianping Wu, Warwick B. Badgery, David R. Kemp

IGC Proceedings (1977-2023)

Meeting demands for livestock products which are predicted to more than double during the next 20 years, is central to the challenge of feeding the world sustainably. Smallholders will play a key role in achieving global security in animal protein. However, this requires a shift from subsistence to market-oriented farming where production efficiency not the number of livestock is the key focus with the aim of producing ‘more from less’. For grassland-based ruminant production, reducing stocking rate from current unsustainable levels under subsistence management is an essential first step to producing more production and profit from fewer animals. This is …

Stakeholders Integration For Sustainable Use Of Temperate Forage/Livestock Agriculture, Garry D. Lacefield, Don Ball

IGC Proceedings (1977-2023)

Forage/livestock production is complex, and best management options are often site-specific, but some concepts have broad application. The intent of this article is to provide an overview of research-based management approaches that the authors believe are necessary to ensure the sustainability of forage/livestock farms. This overview discussion is needed because livestock agriculture is changing, thus creating both challenges and opportunities for producers. In the last 30 years, beef numbers in the USA have declined by 20%, but production per cow has more than doubled, resulting in increased total production on less land with fewer animals. Similar statistics exist for the …

Socio-Cultural Factors Challenging Development Interventions In Cattle Production In The Remote Areas Of Vietnam, Duong-Nam Ha, Pham-Van Hung, Tran-The Cuong, Ninh-Xuan Trung, Tran-Van Long, Laurie Bonney, Peter A. Lane, Guillaume Duteurtre, Stephen Ives

IGC Proceedings (1977-2023)

The northwest highlands of Vietnam are characterized by high altitude, low infrastructure, and low population densities composed of a wide diversity of different ethnic groups. Their socio-cultural characteristics strongly influence their lifestyle and production systems, including agricultural activities. The majority of these people have suffered from slow economic development, with the highest poverty rate in the country. This is a real need for plausible interventions where behavioral changes of smallholders throughout local value chains would be a critical foundation. Our project implemented in this context of development in the Northwest highlands of Vietnam aims to understand the role of socio-cultural …

Changing Grassland Scenario In Developing Countries--Economical And Social Perspective, Abule Ebro

IGC Proceedings (1977-2023)

Grasslands are mainly used for livestock production in developing countries although they have been facing contradictory pressures, i.e., increased demand for natural resources and animal products to cope with rising human populations. On the other, there is a need to preserve the environment and ecosystem. This paper reviewed the causes of grassland changes, the economic and social perspective of changing grasslands with more emphasis on dry lands. Different indicators were used to assess the economic (livestock production, wildlife and tourism, crop production, ecosystem services) and social (traditional institutions, mobility, land tenure and grazing systems) perspectives of changing grassland. The major …

Conservation Of Grassland Plant Genetic Resources Through People Participation, D. R. Malaviya, Ajoy K. Roy, P. Kaushal

IGC Proceedings (1977-2023)

Agrobiodiversity provides the foundation of all food and feed production. Hence, need of the time is to collect, evaluate and utilize the biodiversity globally available. Indian sub-continent is one of the world’s mega centers of crop origins. India possesses 166 species of agri-horticultural crops and 324 species of wild relatives. India is reported to have five grass covers identified. There have been almost nil human interference in terms of selection pressure; hence, the biodiversity is well conserved in these grazing lands. There is need of a system approach to understanding biodiversity that moves significantly beyond taxonomy and species observations. In …

Breeding Strategies To Improve Fodder Legumes With Special Emphasis On Clover And Medics, Ajoy K. Roy, D. R. Malaviya, P. Kaushal

IGC Proceedings (1977-2023)

In India, Forage has a unique integration of agriculture and animal industry in diversified rich cultural environment. Greater part of rural economy in India relies much upon mixed farming system, a well-knit combination of crop production and livestock rearing. In India, grazing-based livestock husbandry plays an important role in the rural economy as around 50% of animals depend on grazing. Pasturelands over an area of 12 Mha constitute the main grazing resources that are available. Nearly 30 pastoral communities in hilly or arid/semi-arid regions in northern and western parts of India, depend on grazing-based livestock production. Nomadic pastoralism, a traditional …

Front Matter [23rd International Grassland Congress Proceedings], National Organizing Committee, International Grassland Congress

IGC Proceedings (1977-2023)

No abstract provided.

Front Matter [Preface And List Of Contributors], National Organizing Committee, International Grassland Congress

IGC Proceedings (1977-2023)

No abstract provided.

Promising Forage Options To Enhance Livestock Production In Mediterranean Climate Agricultural Systems, Serkan Ates, Hayley C. Norman, Hichem Ben Salem, Bradley Nutt, Harun Cicek

IGC Proceedings (1977-2023)

The increasing demand for food, fibre and animal products inevitably requires intensifying agricultural production worldwide. This can present a number of environmental and farming systems challenges in Mediterranean climate areas of the world. Developments of novel strategies, employing ecologically sound intensification practices, are crucial to enhance production in the Mediterranean agropastoral production systems. In this paper, we have discussed forage based systems that improve productivity, profitability, environmental quality and resilience of the farming systems. Key opportunities include better use and integration of diverse genetic resources, new agronomic techniques and the utilization of perennial forage plants that are adapted to land …

New Methodologies For Grasslands Monitoring, Katarzyna Dabrowska-Zielinska, Piotr Goliński, Marit Jorgensen, Jørgen Mølmann, Gregory Taff, Monika Tomaszewska, Barbara Golińska, Maria Budzynska, Martyna Gatkowska

IGC Proceedings (1977-2023)

Monitoring grassland areas to assess changes in their condition over time has been the subject of a lot of research at different scales. Initially the techniques focused on field-based measurements, and modelling. However, several obtained data were site specific. Based on the increase in availability of remote sensing data and products, there is an expectation that remote sensing can provide rapid and definite answers to the challenges of detecting and monitoring grassland conditions and associated changes in productivity. At the time of European Copernicus Programme, the new possibilities of satellite data from the group of Sentinel satellites give the new …

Grazing Management Options For Maintaining Optimum Pasture Composition And Utilization, Glen E. Aiken

IGC Proceedings (1977-2023)

Global demand for meat and dairy products will continue to increase over the 21st century, however, the global forage-based livestock industry will be challenged in meeting production goals with minimal impact of the environment. Sustainable production of grazing livestock will depend on the use of carefully planned grazing management strategies. Development of a grazing management plan involves making two decisions: determining the livestock density at which pastures are stocked and settling on the method used to graze the pastures. Stocking rate indirectly affects output per animal and per hectare through its direct effect on forage mass and pasture composition. A …

Enhancing Grassland Productivity Through Disease Management Of Grass And Forage Species, Tingyu Duan, Chunjie Li, Yanzhong Li, Binhua Yu, Zhibiao Nan

IGC Proceedings (1977-2023)

The total area of grassland in China is about 400 million hectares, accounting for 41.7% of the country’s land area, which plays important role in ecological construction and food security assurance of the national. However, diseases of grass and forage limited the development of the pastoral agriculture. There are more than1500 new diseases were reported in the past 16 years with more and more new diseases were discovered. The damage to grassland is becoming more severe with the known diseases prevalent areas expanding.

Management Of Pasture Soils: Biochar Stability, Carbon Storage Potential And Its Effect On Production And Quality, Bhupinder Pal Singh, Yunying Fang, Mark Boersma, Damian Collins, Lukas Van Zwieten, Lynne M. Macdonald

IGC Proceedings (1977-2023)

The use of biochar has been proposed as a stable carbon (C) amendment with long-term carbon (C) storage potential in agricultural soils while improving primary productivity. However, this concept has not been widely tested in contrasting soils under temperate pasture systems. To address this knowledge gap, a ¹³C-labelled biochar, produced from Eucalyptus saligna biomass by slow pyrolysis (450° C; d¹³C -36.7‰) was surface (0"10 cm) applied in C₃ dominated, annual temperate pasture systems across Arenosol, Cambisol and Ferralsol. The results show that only 2% of the applied biochar-C was mineralised in a relatively clay- and C-poor …

Grassland Renovation And Consequences For Nutrient Management, Johannes Isselstein, Manfred Kayser

IGC Proceedings (1977-2023)

Sward degradation is a serious threat to the functioning of grassland and the provision of ecosystem services. Renovation measures are frequently applied in order to restore degraded swards. However, the success is highly variable and substantial tradeoffs are often found following renovation such as among agronomic and environmental services. Starting from a general classification of renovation measures the paper investigates the processes induced by renovation that lead to a change of the vegetation and that affect carbon and nitrogen fluxes. These processes are strongly interrelated and dependent on site, climate and management condition as well as on the time scale. …

Roles And Programming Of Arabidopsis Argonaute Proteins During Turnip Mosaic Virus Infection, Hernan Garcia-Ruiz, Alberto Carbonell, J. Steen Hoyer, Noah Fahlgren, Kerrigan B. Gilbert, Atsushi Takeda, Annalisa Giampetruzzi, Mayra T. Garcia Ruiz, Michaela G. Mcginn, Nicholas Lowery, Maria T. Martinez Baladejo, James C. Carrington

Department of Plant Pathology: Faculty Publications

In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has ten AGO genes of which AGO1, AGO2, and AGO7 have been shown to play roles in antiviral defense. A genetic analysis was used to identify and characterize the roles of AGO proteins in antiviral defense against Turnip mosaic virus (TuMV) in Arabidopsis. AGO1, AGO2 and AGO10 promoted anti-TuMV defense …

Grassland Resources And Protections In The Yellow River Source Zone On The Qinghai-Tibet Plateau, Xilai Li, Gary J. Brierley, Youming Qiao, George L. W. Perry, Jay King Gao

IGC Proceedings (1977-2023)

This paper summarises resources and protections of the Yellow River Source Zone on the Qinghai-Tibet Plateau. A concerted effort has been made to address concerns for overgrazing on the alpine steppe and alpine meadow landscapes in the source zone of the Yellow River. An assessment of the impacts of overgrazing includes consideration of the role of small mammals (on the one hand they are considered as a critical ecosystem engineer, on the other they are perceived as a major threatening pest). Analyzed in this paper are management options in the restoration of degraded grasslands.

Importance Of Livestock Production From Grasslands For National And Local Food And Nutritional Security In Developing Countries, Iain A. Wright

IGC Proceedings (1977-2023)

Grazing lands cover more than a quarter of the world’s land surface, often on land that is unsuitable for other forms of use. Despite the perception that productivity is inherently low, the contribution of grasslands in food security in developing countries is significant. However the challenges of spatial and temporal variability of primary productivity need to be managed and mobility of livestock is key to this. Appropriate land management and governance arrangements are essential for facilitating this mobility and for creating the circumstances in which technical options for reducing variability and risk in livestock keeping can be deployed and to …