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Du Undergraduate Showcase: Research, Scholarship, And Creative Works, Caitlyn Aldersea, Justin Bravo, Sam Allen, Anna Block, Connor Block, Emma Buechler, Maria De Los Angeles Bustillos, Arianna Carlson, William Christensen, Olivia Kachulis, Noah Craver, Kate Dillon, Muskan Fatima, Angel Fernandes, Emma Finch, Colleen Cassidy, Amy Fishman, Andrea Francis, Stacia Fritz, Simran Gill, Emma Gries, Rylie Hansen, Shannon Powers, Jacqueline Martinez, Zachary Harker, Ashley Hasty, Mykaela Tanino-Springsteen, Kathleen Hopps, Adelaide Kerenick, Colin Kleckner, Ci Koehring, Elijah Kruger, Braden Krumholz, Maddie Leake, Lyneé Alves, Seraphina Loukas, Yatzari Lozano Vazquez, Haley Maki, Emily Martinez, Sierra Mckinney, Audrey Mitchell, Kipling Newman, Audrey Ng, Megan Lucyshyn, Andrew Nguyen, Stevie Ostman, Casandra Pearson, Alexandra Penney, Julia Gielczynski, Tyler Ball, Anna Rini, Christina Rorres, Simon Ruland, Helayna Schafer, Emma Sellers, Sarah Schuller, Claire Shaver, Kevin Summers, Isabella Shaw, Madison Sinar, Claudia Pena, Apshara Siwakoti, Carter Sorensen, Madi Sousa, Anna Sparling, Alexandra Revier, Brandon Thierry, Dylan Tyree, Maggie Williams, Lauren Wols

DU Undergraduate Research Journal Archive

DU Undergraduate Showcase: Research, Scholarship, and Creative Works

Identification Of Signal Transducers In Plant Shoot Gravitropism Using Genetic Screens, Lilla Reed

Honors Theses

Gravitropism is an essential response for plant growth and architecture. The signal transduction pathway that links gravity sensing to gravity response remains unclear because the key signal transducers involved in this pathway are unknown. In this project, we applied an artificial microRNA(amiRNA)-assisted genetic screen in isolating mutants displaying altered gravitropic phenotype in the darkness. Two amiRNA lines showed a significantly decreased gravitropic phenotype in the hypocotyl, and amiRNAs in these lines were identified to target a hypothetical transmembrane protein and a receptor kinase, respectively. Further experiments need to be done to confirm their phenotypes and establish them as signal transducers …

Hybrid Allele‑Specific Chip‑Seq Analysis Identifies Variation In Brassinosteroid‑Responsive Transcription Factor Binding Linked To Traits In Maize, Thomas Hartwig, Michael Banf, Gisele Passaia Prietsch, Jia‑Ying Zhu, Isabel Mora‑Ramírez, Jos H. M. Schippers, Samantha J. Snodgrass, Arun S. Seetharam, Bruno Huettel, Judith M. Kolkman, Jiashi Yang, Julia Engelhorn, Zhi‑Yong Wang

Department of Agronomy and Horticulture: Faculty Publications

Background: Genetic variation in regulatory sequences that alter transcription factor (TF) binding is a major cause of phenotypic diversity. Brassinosteroid is a growth hormone that has major effects on plant phenotypes. Genetic variation in brassinosteroidresponsive cis-elements likely contributes to trait variation. Pinpointing such regulatory variations and quantitative genomic analysis of the variation in TF-target binding, however, remains challenging. How variation in transcriptional targets of signaling pathways such as the brassinosteroid pathway contributes to phenotypic variation is an important question to be investigated with innovative approaches.

Results: Here, we use a hybrid allele-specific chromatin binding sequencing (HASChseq) approach and …

Hymenachne Amplexicaluis [(Rudge) Nees] Genetic Resources Collection In México, A Suitable Grass For Flood Plains In Tropical Areas, J. F. Enriquez-Quiroz, A. R. Quero-Carrillo, J. Perez-Perez, A. Hernandez-Garay, E. Garcia-Moya

IGC Proceedings (1977-2023)

Hymenachne amplexicaluis [( Rudge ) Nees; 2n= 2x= 24; Azuche, West Indian marsh grass] is a native Central and South America C₃ grass that grows well under intermittent flooding conditions. It produces good seed set and stolons to thrive on new areas assuring its survival, combined with an efficient N metabolism to promote vigorous new growing leaves and tillers (Antel et al., 1998). Azuche is a dual attribute species when introduced to new areas; it has valuable forage attributes but also is a potential weed (Hill, 2000). As Azuche is a native species, one must deal with in …

Critical Analysis Of Tropical Forage Breeding In Brazil, J. A. Usberti Jr., P. B. Alcantara

IGC Proceedings (1977-2023)

Forage grasses account for 90% of the Brazilian forage seed market while the genera Brachiaria, Panicum and others are responsible for 85, 10 and 5% of the traded grass seeds, respectively. Most of the forage grass and legume cultivars available for sowing in Brazil were selected in germplasm banks during the last 20 years, while few of them were derived from artificial crossings, followed by selection for desirable forage traits. The selection of new genetic materials in germplasm banks (exploitation of naturally-occurring genetic variability) is still feasible but the chances of success are decreasing through time. From now on, …

Patterns And Sources Of Variation In Heterospecific Pollen Deposition In Flowers Of The Native Blue Cardinal Flower (Lobelia Siphilitica), Allie Drinnon

Undergraduate Honors Theses

Plants species interactions via pollinators are a model system to understand the mechanisms that generate plant diversity in nature. However, most studies have focused on plant-plant interactions via pollinator attraction while ignoring the role of plant-plant interactions via pollen transfer. Heterospecific pollen transfer (henceforth HP) can be common and have negative fitness effects. Negative HP fitness effects may prompt the evolution of adaptive strategies to minimize them. However, the extent of spatial variation in HP load size within and among populations, a tenet for natural selection, remains unexplored. Such knowledge would hence constitute a first step in advancing our understanding …

Evaluating Planting Green And Herbicides For Integrated Weed Management And Their Effect On Soil Properties In Corn And Soybean In Nebraska, Trey Parker Stephens

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Producers across the Midwest are finding new ways to implement cover crops into cropping systems and the practice of “Planting Green” is one of the newest uses of cover crops. When planting green, producers plant their row crops into actively growing cover crops and terminate the cover crop at time of planting or shortly after planting. This practice would allow for higher biomass accumulation of the cover crop and could aid in weed management of herbicide-resistant weeds. The objective of the first two studies was to evaluate planting green and its effect on soil-applied residual herbicides, weed management, dicamba/glyphosate-resistant soybean …

Genomic Selection For Yield And Seed Composition Stability In An Applied Soybean Breeding Program, Benjamin Mark Harms

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Stability traits are of primary importance in plant breeding to ensure consistency in phenotype across a range of environments. However, selection efficiency and accuracy for stability traits can be hindered due to the requirement of obtaining phenotype data across multiple years and environments for proper stability analysis. Genomic selection is a method that allows prediction of a phenotype prior to observation in the field using genome-wide marker data and phenotype data from a training population. To assess prediction of stability traits, two elite-yielding soybean populations developed three years apart in the same breeding program were used. The individuals in each …

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …

Interactive Map Code (R And Rstudio), Peter G. Butterfield

Data Files: White Oaks in Vinton Furnace Experimental Forest

Interactive map code puts data and coordinates into readable table format and creates data visualizations.

Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo

Honors Program: Senior Projects (Public)

Nitrogen plays a major role in the proper growth and development of maize and is therefore essential to crop production, being the most critical nutrient for achieving optimal yield. In previous field and greenhouse studies, we have found maize lines that differ in their resiliency to nitrogen stress. To identify the potential genomic regions associated with the differences in nitrogen deficiency resilience, Genome-Wide Association Studies (GWAS) were conducted. Based on a previous study, the most consistently resilient maize inbred lines within the Goodman-Buckler diversity panel have been identified as the lines A619 and A661, while the non-resilient inbred lines have …

Meta-Analysis Of The Effects Of Endophytes And Their Secondary Metabolites On Herbivory From Insects, Derrick Taylor

All Theses

Endophyte-infected plants have reduced the level of herbivory. The defense to herbivory can vary due to the metabolites in the plant and the method of feeding from the pest. Secondary metabolites produced by the endophytes are mainly alkaloids. The associations between endophytes and some alkaloid groups are studied in greater detail. The lack of research of certain alkaloid groups ultimately means that some alkaloids had more data that could be analyzed. As a result, the alkaloid groups were not evenly represented in the meta-analysis. The meta-analysis analyzed the relative effectiveness of the metabolites or endophytes in each of the articles …

Non-Destructive Imaging Of Phytosulfokine Trafficking In Plants Using Fiber-Optic Fluorescence Microscopy, Bernard Abakah

Electronic Theses and Dissertations

Plants secrete peptide ligands and use receptor signaling to respond to stress and control development. Understanding these phenomena is key to improving plant health and productivity for food, fiber, and energy applications. Phytosulfokine (PSK), a sulfated peptide hormone, regulates plant cell division, growth, and stress tolerance via specific phytosulfokine receptors (PSKRs). This study uses fiber-optic fluorescence microscopy to elucidate trafficking of PSK in live plants. The microscope features two-color optics and an objective lens connected to a 1-m coherent imaging fiber mounted on either a conventional upright microscope body or 5-axis positioning system (X–Y–Z plus pitch and yaw). PSK and …

Investigating The Impact Of Edaphobacter Aggregans (Wbg-1) On Arabidopsis Thaliana Development, Julia Talbert

Undergraduate Research Symposium

Acidobacteria is a phylum of bacteria that make up 50% of soil microbes. While making up a large proportion of bacteria found in soil, this phylum is largely unexplored with limited information about the bacteria and their impact on plant growth. The selected target of this study is Edaphobacter aggregans, WBG-1, which is a part of a subdivision of acidobacteria. Examining the bacteria’s effects on the development of the model plant Arabidopsis thaliana will provide insight into understanding this unknown phylum of soil-dwelling bacteria. Acidobacteria are an important realm to investigate as they affect agriculture practices. With Missouri being such …

Sensing Is A Far Cry From Sentience, Antonio Damasio, Hanna Damasio

Animal Sentience

The hypothesis that plants might be sentient confuses the notion of sentience (or consciousness) with that of sensing. Sentience/consciousness implies feeling, experience, and subjectivity. Sensing does not. Plants can sense/detect and even respond appropriately in the absence of any sentience/consciousness.

Impact Of Drought And Fertilizer Use On The Photosynthesis Rate Of Cucumber And Sweet Corn Under Ambient And Elevated Atmospheric Co2 Conditions, Kate Fenske

Celebrating Scholarship and Creativity Day (2018-)

No abstract provided.

A Deep Learning Framework For Processing And Classification Of Hyperspectral Rice Seed Images Grown Under High Day And Night Temperatures, Víctor Díaz-Martínez, Jairo Orozco-Sandoval, Vidya Manian, Balpreet K. Dhatt, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A framework combining two powerful tools of hyperspectral imaging and deep learning for the processing and classification of hyperspectral images (HSI) of rice seeds is presented. A seed-based approach that trains a three-dimensional convolutional neural network (3D-CNN) using the full seed spectral hypercube for classifying the seed images from high day and high night temperatures, both including a control group, is developed. A pixel-based seed classification approach is implemented using a deep neural network (DNN). The seed and pixel-based deep learning architectures are validated and tested using hyperspectral images from five different rice seed treatments with six different high temperature …

Entropy And Energy Profiles Of Chemical Reactions, Johannes C. B. Dietschreit, Dennis J. Diestler, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

The description of chemical processes at the molecular level is often facilitated by use of reaction coordinates, or collective variables (CVs). The CV measures the progress of the reaction and allows the construction of profiles that track the evolution of a specific property as the reaction progresses. Whereas CVs are routinely used, especially alongside enhanced sampling techniques, links between profiles and thermodynamic state functions and reaction rate constants are not rigorously exploited. Here, we report a unified treatment of such reaction profiles. Tractable expressions are derived for the free-energy, internal-energy, and entropy profiles as functions of only the CV. We …

Plant Sentience: A Hypothesis Based On Shaky Premises, Carel Ten Cate

Animal Sentience

Plants may produce fascinating behavioural phenomena for which the label ‘cognitive process’ may be applicable, at least by some definitions. Segundo-Ortin & Calvo (2023) base their hypothesis that plants might be sentient on the premise of demonstrated presence of cognitive complexity. However, the way phenomena are ascribed, and how the term ‘cognitive’ is used by Segundo-Ortin & Calvo, deviates from the common practice in studies of animal cognition, implying greater complexity than seems justified. It thus provides a questionable basis for attributing sentience to plants.

The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran

Undergraduate Theses

Animal pollinators are the cornerstone of healthy ecosystems. Their survival is essential for the persistence of entire food chains: from the flowers they cross-pollinate directly, to the animals who depend on those plants for nutrition. The establishment of pollinator gardens—particularly ones that consist of native plants—is an effective way to enhance their biodiversity, abundance, and well-being.

The main goal of this thesis is to construct a pollinator garden that maximizes the benefits for animal pollinators using feedback from local gardeners. A survey was used to gather information about the popularity and preferences of 40 flowering plants, and after analyzing the …

Divergent Metabolic Changes In Rhizomes Of Lowland And Upland Switchgrass (Panicum Virgatum) From Early Season Through Dormancy Onset, Nathan A. Palmer, Gautam Sarath, Michael J. Bowman, Aaron J. Saathoff, Serge J. Edmé, Robert B. Mitchell, Christian M. Tobias, Soundararajan Madhavan, Erin D. Scully, Scott E. Sattler

Department of Agronomy and Horticulture: Faculty Publications

High-biomass-yielding southerly adapted switchgrasses (Panicum virgatum L.) frequently suffer from unpredictable winter hardiness at more northerly sites arising from damage to rhizomes that prevent effective spring regrowth. Previously, changes occurring over the growing season in rhizomes sampled from a cold-adapted tetraploid upland cultivar, Summer, demonstrated a role for abscisic acid (ABA), starch accumulation, and transcriptional reprogramming as drivers of dormancy onset and potential keys to rhizome health during winter dormancy. Here, rhizome metabolism of a high-yielding southerly adapted tetraploid switchgrass cultivar, Kanlow—which is a significant source of genetics for yield improvement—was studied over a growing season at a northern …

Differential Behaviour Of Guineagrass (Panicum Maximum Jacq.) Hybrids, With Different Al+3 Reactions, As To Major Nutrient Translocations To The Leaves, R. S. Paterniani, J. A. Usberti Jr., J. C. Werner

IGC Proceedings (1977-2023)

Most of the Brazilian cultivated pasture fields presents soils with high Al⁺³ levels and liming is economically unfeasible. So, there is an urgent need for grasses with good forage yielding potentials that can withstand Al⁺³ deleterious effects (abnormal root development: short, thick and poorly branched roots, which are unable to effectively translocate water and essential nutrients to the leaves) (Foy, 1984); as a consequence, susceptible genetic materials have their field persistences greatly affected, mainly during drought periods. Researches on Al⁺³ reaction are usually compare supposedly resistant/tolerant genotypes with a resistant control check; doing so, the genotypic effect …

Animal Performance And Productivity Of New Ecotypes Of Brachiaria Brizantha In Brazil, Valéria P. B. Euclides, Manuel C. M. Macedo, Cacilda B. Do Valle, R. Flores, M. P. Oliveira

IGC Proceedings (1977-2023)

Brazil has the competitive advantage of a very dynamic and cost effective animal production system on pastures over other countries. The pursuit for more productive forages that will result in higher quality beef at a lower cost is then justified. Brachiaria is the most important forage genus utilised in Brazil, thus an intense search for new cultivars amongst collected and introduced ecotypes from Africa is underway. Following agronomic evaluation of this material in plots, 8 pre-selected Brachiaria ecotypes were tested under intermittent grazing in paddocks (Euclides et al., 2001). Continuing on the process of cultivar development, two out of …

Development Of A Bahiagrass Paspalum Notatum Flugge With Increased Short-Day Biomass, P. Mislevy, Ann R. Blount, T. R. Sinclair

IGC Proceedings (1977-2023)

Low herbage productivity of subtropical grasses during the short-day winter months of October through to March can place a severe burden on livestock producers in Southeastern U.S. Researchers at the University of Florida (Sinclair et al., 2001) hypothesised that the decrease in forage production might result from physiological dormancy induced by short day length. A study using artificial lights to extend the day length demonstrated that maintaining the day length at 15 hr during the short-day length period increased 'Pensacola' bahiagrass P. notatum Flugge saure Parodi forage yield 122% when compared with normal photoperiod (Mislevy et al., 2001). …

Genetic And Molecular Characterization Of Temperate And Tropical Forage Maize Inbred Lines, B. Alarcón-Zúñiga, E. Valadez-Moctezuma, T. Cervantes-Martinez, T. Cervantes-Santana, M. Mendoza‐Rodriguez

IGC Proceedings (1977-2023)

The livestock feeding in the Central highland of Mexico is based on harvest, grazing and annual forage conservation, being forage maize the most important silage crop (Alarcón, 1995). Even though forage maize is extensively bred in Europe, USA and Asia since 1900's, this started in Mexico in the 1960's, and little is known about the genetic diversity in both agronomic and nutritive value traits. Our breeding program goals are to analyze combining ability of biomass and quality predictors and to study the genetic relationship of inbred lines between lowland tropical and temperate races from Mesa Central, by genetic and molecular …

Preliminary Performance Of Panicum Maximum Accessions And Hybrids In Brazil, Liana Jank, Rosangela M. S. Resende, S. Calixto, M. M. Gontijo Neto, V. A. Laura, Manuel C. M. Macedo, Cacilda B. Do Valle

IGC Proceedings (1977-2023)

Due to the lack of high quality forages adapted to the distinct ecosystems in Brazil, a national network to evaluate 14 accessions, 4 hybrids and 5 commercial standards of Panicum maximum was established in 2002, in 5 regions of Brazil (states of Mato Grosso do Sul, Acre, Rondônia, Minas Gerais and in the Federal District). Staff of the Embrapa Beef Cattle group co-ordinated the network and pre-selected the accessions and hybrids (Jank, 1995; Jank et al., 2001; Resende et al., 2004). We present their performance as to leaf dry matter yield (LDMY) and leaf percentage (LP) obtained from …

Effect Of Applied Biosolids To Bahiagrass Pastures On Copper Status Of Cattle, M. E. Tiffany, L. R. Mcdowell, G. A. O'Connor, N. S. Wilkinson

IGC Proceedings (1977-2023)

When grazing ruminants consume forages high in Mo but adequate in S, there is a risk of molybdenosis (a Mo-induced Cu deficiency). This occurs when Mo, S, and Cu join to form Cu-thiomolybdate complexes in the rumen that are not readily absorbed (Suttle, 1991). High dietary S reduces Cu absorption, possibly due to unabsorbable Cu sulphide formation, independent from its part in thiomolybdate complexes. The use of municipal sludge (biosolids) as a pasture fertiliser is of interest since some contain high Mo which may induce Cu deficiency. The objective of this study was to evaluate the performance and Cu status …

An In Vitro Investigation Of Forage Factors Which Affect The Production Of Conjugated Linoleic Acid And Trans Vaccenic Acid In The Rumen. Ii. Wilting & Cell Damage, Michael R. F. Lee, J. K. S. Tweed, Nigel D. Scollan, R. J. Dewhurst

IGC Proceedings (1977-2023)

Previous studies have shown that cis 9 trans 11 conjugated linoleic acid (CLA) concentrations are higher for summer milk produced from cows grazing fresh pastures than for winter milk when conserved forages are fed (Jahreis et al., 1997). Furthermore, Offer (2003) showed that a similar depression in milk fat CLA occurred if grass was simply cut and fed after a short wilt. This experiment investigated the effect of wilting on the production of CLA and trans vaccenic acid (TVA) in an in vitro rumen simulation, and whether any differences could be related to changes in plant structure. It was …

Effects Of Grassland Management On Herbage Lipid Composition And Consequences For Fatty Acids In Milk, Anjo Elgersma, P. Maudet, I. Witkowska, A. C. Wever

IGC Proceedings (1977-2023)

Herbage provides bulk feed and is the basis for ruminant nutrition. Herbage lipids, especially C18:3, are a major source of beneficial fatty acids (FA) in milk. These desired FA are unsaturated FA such as CLA (conjugated linoleic acid), especially the isomer rumenic acid, and also vaccenic acid, both trans omega-7 FA (Ellen & Elgersma, 2004). As information on lipids in forages is scarce, effects were studied of N application level and regrowth period on the lipid concentration and FA composition of perennial ryegrass (Lolium perenne L.), the most important forage in temperate climate zones. A linear relation had previously …

Effect Of Feeding L-Carnitine And Sunflower Seeds On Cla Content Of Pasture-Fed Beef, S. L. Scott, W. P. Mccaughey, K. E. Buckley

IGC Proceedings (1977-2023)

Pasture finishing enhances levels of conjugated linoleic acids (CLA) in beef lipids (Shanta et al. 1997). CLA (e.g., C18:2 c9, t11), formed during biohydrogenation of polyunsaturated fatty acids (PUFA) in the rumen, can reduce the incidence of heart disease, cancer and obesity in humans. However, pasture finishing cattle can reduce carcass grade. Feeding pasture-fed cattle a high-grain diet for a short finishing period (~60 d) improves grades but may reduce lipid CLA levels. A feeding regime is required that maintains the positive nutritional attributes of pasture-fed beef and improves the meat grade. The objective of this study was to investigate …