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Ecology And Invasive Properties Of Musk Thistle (Carduus Nutans) In The Central Prairies Of Nebraska, Chengchou Han

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Musk thistle (Carduus nutans) is an herbaceous monocarpic herb introduced to the U. S. from Eurasia. The invasion of musk thistle can reduce forage area, soil stability, and reduce recreation and open areas for humans and wildlife.

Resistance of warm season and cool season perennial grass communities to musk thistle invasion is important for land managers to consider, especially where disturbance has made an area particularly susceptible. Our results show that disturbances, such as overgrazing can open up niches in canopies of warm season grass communities and facilitate invasion but not in cool season grass communities. The mechanism …

The Biological Control Of Spotted Knapweed In The Southeastern United States, Carey R. Minteer

Graduate Theses and Dissertations

Spotted knapweed is an invasive, short-term-perennial plant that is native to Eurasia. It was accidentally introduced into North America in the early 1890's and has since spread across The United States and Canada. Spotted knapweed degrades rangelands and pastures by negatively impacting native plants, increasing soil surface runoff and stream sediment yields, and reducing soil infiltration. A biological control program for spotted knapweed using Larinus minutus (Coleoptera: Curculionidae), was initiated in Arkansas in 2008. In this dissertation I described the releases of L. minutus and investigated the adult activity in the southeastern United States (Chapter 1), investigated the effects of …

Taxonomy And Dna Barcoding In The Genus Manfreda (Salisb.) Asparagaceae, William David Ritchie

Graduate Theses and Dissertations

The genus Manfreda Salisb. of Asparagaceae is a genus of potential horticultural interest and is currently subject to breeding efforts at the University of Arkansas. A lack of taxonomic clarity however undermines the classification of potential inter - and intrageneric hybrids. The study aims to assess existing species delimitation within the genus Manfreda employing morphology while investigating the potential utility of Consortium for the Barcode of Life Plant (CBOL) DNA Barcodes for identification of specific taxa and an External Transcribed Spacer (ETS) - Internal Transcribed Spacer (ITS) DNA barcode for developed hybridized taxa.

Observation of 855 herbarium specimens facilitated phylogenetic …

Evaluation Of The Recombination Efficiencies Of Flp Proteins, Linh Duy Nguyen

Graduate Theses and Dissertations

Site-specific recombination systems are powerful tools for genetic modification. They have been used to integrate a transgene into a pre-defined locus and to remove marker genes from a transgene locus. Two of the most widely used site-specific recombination systems in plants are the Cre/lox system from the bacteriophage P1 and the FLP/FRT system from the yeast Saccharomyces cerevisiae. The Cre/lox system is well-characterized and is the first choice in application of site-specific recombination system. However, some applications such as marker-free site-specific gene integration require the use of two recombination systems. In addition, the availability of alternative recombination systems can offer …

False Smut Of Rice: Histological Analysis Of Infection, Liem Thi Thanh Nguyen

Graduate Theses and Dissertations

False smut of rice, caused by Ustilaginoidea virens (Cooke) Takahashi (Teleomorph: Villosiclava virens), has become a common disease in most major rice growing regions throughout the world. Considerable confusion exists regarding the infection process and the disease cycle. Therefore, a clearer understanding of pathogenesis caused by Ustilaginoidea virens is critical for future efforts to develop genetic and chemical tools to manage false smut in Arkansas and other regions of the world. The overall goal of this research was to clarify the infection process underlying false smut, with emphasis on comparing and contrasting the histological basis of root and foliar infections. …

Challenges In Predicting The Future Distributions Of Invasive Plant Species, Chad C. Jones

Botany Faculty Publications

Species distribution models (SDMs) are increasingly used to predict distributions of invasive species. If successful, these models can help managers target limited resources for monitoring and controlling invasive species to areas of high invasion risk. Model accuracy is usually determined using current species distributions, but because invasive species are not at equilibrium with the environment, high current accuracy may not indicate high future accuracy. I used 1982 species distribution data from Bolleswood Natural Area, Connecticut, USA, to create SDMs for two forest invaders, Celastrus orbiculatus and Rosa multiflora. I then used more recent data, from 1992 and 2002, as validation …

Situation Statement: The 'Prickle Bush' Weeds (Mesquite, Parkinsonia And Prickly Acacia) In Western Australia, Jon Dodd, Andrew Reeves, Richard Watkins, Linda Anderson

Biosecurity published reports

The term 'Prickle bush weeds' refers to weedy trees and shrubs of Mesquite, Parkinsonia and Prickly acacia. All are present in Western Australia.

Mesquite and Parkinsonia are widespread, well established woody weeds in various parts of the WA Rangelands. Prickly acacia is a recent introduction and has a relatively restricted distribution in the East Kimberley. All three impact mainly on the pastoral industry and have considerable potential for further spread and greater impact within the Rangelands. All are Weeds of National Significance (WoNS) and are high priority Declared Plants in WA.

The purpose of this situation statement is to describe …

Marketing Contracts, Overconfidence, And Timing In The Canadian Wheat Market, Fabio L. Mattos, Stefanie A. Fryza

Department of Agronomy and Horticulture: Faculty Publications

This paper investigates factors that impact marketing performance in the Canadian wheat market. Using data provided by the Canadian Wheat Board (CWB) for six crop years, results indicate that producers were not able to profitably use all marketing contracts offered by the CWB, earlier pricing tended to generate better performance, there was a negative relationship between activeness and performance (suggesting overconfidence in marketing skills), and performance was generally worse in volatile crop years. Further analysis reveals some of these findings differ when outperforming and underperforming producers are investigated separately, particularly with respect to activeness and volatility.

Streptomycin Resistance Of Erwinia Amylovora, Causal Agent Of Fire Blight, Claudia Nischwitz

All Current Publications

No abstract provided.

A Proposed Mechanism For Nitrogen Acquisition By Grass Seedlings Through Oxidation Of Symbiotic Bacteria, James F. White Jr., Holly Crawford, Mónica S. Torres, Robert Mattera, Ivelisse Irizarry, Marshall Bergen

All Faculty Scholarship for the College of the Sciences

In this paper we propose and provide evidence for a mechanism, oxidative nitrogen scavenging (ONS), whereby seedlings of some grass species may extract nitrogen from symbiotic diazotrophic bacteria through oxidation by plant-secreted reactive oxygen species (ROS). Experiments on this proposed mechanism employ tall fescue (Festuca arundinaceae) seedlings to elucidate features of the oxidative mechanism. We employed 15N₂ gas assimilation experiments to demonstrate nitrogen fixation, direct microscopic visualization of bacteria on seedling surfaces to visualize the bacterial oxidation process, reactive oxygen probes to test for the presence of H₂O₂ and cultural experiments to assess conditions …

The Relationship Between Leaf Mechanical Strength And Photosynthetic Rates, Aaron Van Loon, Brianna Manes, Taylor Stucky, Thomas Addington

Featured Research

Photosynthesis is the process by which plants convert light energy to chemical energy, helping sustain life in the biosphere. There are many factors that affect the rate of photosynthesis such as atmospheric CO2 concentration, temperature, and sunlight. In this experiment, we attempt to determine what effect, if any, the mechanical strength of a plant has on its rate of photosynthesis. To perform this experiment we used both the LI-COR 6400 and the Instron Mechanical Testing Device. With the LI-COR we measured photosynthetic rate of our plant subjects in real time during the prime photosynthetic hours, 10-12am. The Instron allowed us …

Effect Of Ambient Air Temperature On Leaf Size In Raphanus Sativus, Matthew Fleming, Michael Mccormack

Featured Research

Global warming is an increasing problem in today’s society. Thus, it is important to know how plants, specifically those that are cultivated for human consumption, react to rising average temperatures. This experiment tested how the leaf size of a common crop plant, Raphanus sativus (radish), is affected by two different temperatures. It is hypothesized that as temperature rises, the leaf size will increase, giving the leaf greater ability to cool via transpiration because of an larger surface area for stomatal conductance. This hypothesis was tested by growing two samples of plants in separate incubators. One sample was grown at a …

Fuzzy Clustering Of Cpp Family In Plants With Evolution And Interaction Analyses, Tao Lu, Yongchao Dou, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Transcription factors have been studied intensively because they play an important role in gene expression regulation. However, the transcription factors in the CPP family (cystein-rich polycomb-like protein), compared with other transcription factor families, have not received sufficient attention, despite their wide prevalence in a broad spectrum of species, from plants to animals. The total number of known CPP transcription factors in plants is 111 from 16 plants, but only 2 of them have been studied so far, namely TSO1 and CPP1 in Arabidopsis thaliana and soybean, respectively.

Methods: In this work, to study their functions, we applied …

Some Like It Hot, Some Like It Warm: Phenotyping To Explore Thermotolerance Diversity, C. H. Yeh, Nicholas J. Kaplinsky, C. Hu, Y. Y. Charng

Biology Faculty Works

Plants have evolved overlapping but distinct cellular responses to different aspects of high temperature stress. These responses include basal thermotolerance, short- and long-term acquired thermotolerance, and thermotolerance to moderately high temperatures. This ‘thermotolerance diversity’ means that multiple phenotypic assays are essential for fully describing the functions of genes involved in heat stress responses. A large number of genes with potential roles in heat stress responses have been identified using genetic screens and genome wide expression studies. We examine the range of phenotypic assays that have been used to characterize thermotolerance phenotypes in both Arabidopsis and crop plants. Three major variables …

The Effect Of Leaf Trichome Density On Stem Mechanical Strength In Salvia Leucophylla, S. Mellifera, And S. Apiana, Brieanna English, Jeff Scanlon, Anushree Mahajan

Featured Research

Salvia species in southern California exhibit a variety of leaf trichome densiIes. S. mellifera, S. leucophylla, and S. apiana were chosen as study organisms because they exhibit varying trichome densiIes. A UniSpec was used to measure NDVI in leaves and an Instron was used to measure stem mechanical strength. This study provides preliminary evidence that suggests plants with high leaf trichome density have less stem mechanical strength, and those with low leaf trichome density have greater mechanical strength. Data generated from this study supports the idea that this may be a general and loose trend amongst plant families similar to …

Photosynthetic Advantage Of Invasive Species, Gabby Gurule-Small, Alis Sokolova, Patrick Stephens

Featured Research

Californians have greatly benefited from the introduction of plant and animal species necessary for food or other human pursuits; however, there are many other introduced species that can wreak havoc on the state’s environment and economy. Invasive species threaten the diversity and abundance of native species by both competing for resources and causing changes to the natural habitat. We hypothesize that invasive species will have higher photosynthetic and conductance rates which contribute to their success. Through their impacts on natural ecosystems, agricultural lands, and water delivery systems, invasive species may also negatively affect human health and possibly even the economy.

Is There A Correlation Between Water Potential And Mechanical Strength Of Stems Of Malosma Laurina?, William Chang, Lauren Price, Nicolas Pak

Featured Research

The purpose of this experiment was to see if there was a correlation between water potential and the stem mechanical strength of the Malosma laurina. This experiment was conducted with the use of a Scholander-Hammel pressure chamber to find water potential and the Instron 5500 to test the mechanical strength of the stems. After analysis of the data, there was no direct correlation between water potential and the mechanical strength of stems of Malosma laurina.

The Relationship Of Stomatal Conductnace To Mechanical Strength In Leaves Of Santa Monica Plants, Russell P. Mott, Steven R. Fleming, John R. Macbeth

Featured Research

The Santa Monica Mountains ecosystem has a high diversity of plants with different lifestyles that produce different physiological characteristics individual to all plants. Studies in Australia, another Mediterranean ecosystem, have shown that mechanical strength of leaves is relatable to soil stress. This experiment seeks to determine whether mechanical strengths of leaves correlate to stomatal conductance of leaves across different species in the Santa Monica Mountains. Four species of plants are tested for their stomatal conductance in the field, and the leaves are tested for tensile strength using Young’s Modulus for comparison across leaves. These data show that there was no …

Stomatal Conductance Trends Of The Jade Plant (Crassula Ovata) In Relation To Circadian Rhythm Entrainability, Katie Cahoon, Martin Garcia, Matthew Pierga

Featured Research

The effect of an inverse light cycle on a Crassulacean acid metabolism (CAM) plant was observed in the study. CAM plants are unique in that they open their stomata at night in order to conserve water, an evolution that has come about because these plants primarily exist in very arid climates. By placing a plant in a chamber in which the lights could be programmed to turn on when it was dark out, and to turn off during normal daylight hours, the stomatal conductance of the leaves of a jade plant (Crassula ovata) were recorded several times a day over …

The Effect Of Dirt On Inhibition Of Light Absorption In Musa Leaves, Lorelle Knight, Alexis Carrington, Angela French, Roxanne Barker

Featured Research

Dirt can be transported by wind, human activity and many other factors. It was hypothesized that dirt particles collected on leaves will decrease leaf reflectance and thus make the leaf less healthy. The rationale for this is that the more dirt present on the leaf, the more inhibited photons will be in reaching leaf pigments. The leaf will therefore be less healthy as it will be less able to perform photosynthesis. To test this hypothesis, eight leaf samples with varying amounts of dirt present, were collected from the plant, Musa. A Unispec spectrophotometer was used to test the reflectance of …

The Effect Of Riparian And Arid Environments On Stomatal Conductance In Baccharis Salicifolia And Heteromeles Arbutifolia, Danalit Rangel, Vicki Mac, Ariel Lan

Featured Research

A riparian environment is characterized by higher moisture levels than an arid environment; therefore they have different species of plants that can adapt to their natural habitats. It is critical that we explore the characteristics plants have in relation to their native environments. We propose to test the hypothesis that Baccharis salicifolia would have a higher stomatal conductance rate to water vapor loss compared to Heteromeles arbutifolia because Baccharis salicifolia thrives in a riparian environment in which water abundance would increase stomatal opening thereby contributing to the greater conductance. Using the LI-6400, we measured the conductance rate, photosynthetic rate, CO2 …

A Comparison Of Brittlebush (Encelia Farinosa) Productivity And Health During Drought And Post Rainfall, Gabi Diciolli, Karre Lawson, Anders Reimer

Featured Research

Encelia farinosa is well adapted for drought tolerance as its leaves transform color based on water availability. We were puzzled by a question that any desert botanist must consider. How does the photosynthetic efficiency of E. farinosa fluctuate as it transitions from a state of drought to a state of soil saturation within a 72 hour period? In this experiment we looked at the effects of irrigation on the health and quantum yield of the brittlebush by looking at photosynthetic rates using the portable photosynthesis system LI-COR 6400 when applied to two leaves on a given plant and water potential …

Functions Of The Arabidopsis Kinesin Superfamily Of Microtubule-Based Motor Proteins, Chuanmei Zhu, Ram Dixit

Biology Faculty Research

Plants possess a large number of microtubule-based kinesin motor proteins. While the kinesin-2, 3, 9, and 11 families are absent from land plants, the kinesin-7 and 14 families are greatly expanded. In addition, some kinesins are specifically present only in land plants. The distinctive inventory of plant kinesins suggests that kinesins have evolved to perform specialized functions in plants. Plants assemble unique microtubule arrays during their cell cycle, including the interphase cortical microtubule array, preprophase band, anastral spindle and phragmoplast. In this review, we explore the functions of plant kinesins from a microtubule array viewpoint, focusing mainly on Arabidopsis kinesins. …

Evolution On The Hawaiian Hotspot: Biogeography And Divergence Time Estimation Of Kadua (Rubiaceae), Kenneth L. Parker

Biological Sciences Theses & Dissertations

Recent studies on the colonization time of Hawaiian plant and animal lineages have suggested certain lineages arrived to the archipelago before the formation of the extant islands. Kadua, a recently resurrected genus, is mostly endemic to the Hawaiian Archipelago, with many member taxa autochthonous to individual islands. This work investigates the colonization time of Kadua to the Hawaiian Islands. Sequence analyses of the quickly evolving nuclear regions ITS, ETS and 5s-NTS regions for 20 Hawaiian and 7 French Polynesian species of Kadua were used to create dated phylogeny calibrated with island ages. The phylogeny suggests a single colonization of Hawaii …

Arabidopsis Bhlh100 And Bhlh101 Control Iron Homeostasis Via A Fit-Independent Pathway, Alicia B. Sivitz, Victor Hermand, Catherine Curie, Grégory Vert

Dartmouth Scholarship

Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is known about the signaling pathways and networks driving the various responses to low iron. Only the basic helix–loop–helix (bHLH) transcription factor FIT has been shown to control the expression of the root iron uptake machinery genes FRO2 and IRT1. Here, we characterize the biological role of two other iron-regulated transcription factors, bHLH100 and bHLH101, in iron homeostasis. …

Functional Analysis Of Three Arabidopsis Argonautes Using Slicer-Defective Mutants, Alberto Carbonell, Noah Fahlgren, Hernan Garcia-Ruiz, Kerrigan B. Gilbert, Taiowa A. Montgomery, Tammy Nguyen, Josh T. Cuperus, James C. Carrington

Department of Plant Pathology: Faculty Publications

In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), destabilization through slicerindependent turnover mechanisms, and translational repression. Here, wild-type and active-site defective forms of several Arabidopsis thaliana AGO proteins involved in posttranscriptional silencing were used to examine several AGO functions, including small RNA binding, interaction with target RNA, slicing or destabilization of target RNA, secondary small interfering RNA formation, and antiviral activity. Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects …

Rosette Iron Deficiency Transcript And Microrna Profiling Reveals Links Between Copper And Iron Homeostasis In Arabidopsis Thaliana, Brian M. Waters, Samuel A. Mcinturf, Ricardo J. Stein

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) is an essential plant micronutrient, and its deficiency limits plant growth and development on alkaline soils. Under Fe deficiency, plant responses include up-regulation of genes involved in Fe uptake from the soil. However, little is known about shoot responses to Fe deficiency. Using microarrays to probe gene expression in Kas-1 and Tsu-1 ecotypes of Arabidopsis thaliana, and comparison with existing Col-0 data, revealed conserved rosette gene expression responses to Fe deficiency. Fe-regulated genes included known metal homeostasis-related genes, and a number of genes of unknown function. Several genes responded to Fe deficiency in both roots and rosettes. …

High School Horticulture Curriculum, Margaret Maratsos

Horticulture and Crop Science

Horticulture is just one of the many topics covered in a high school agriculture curriculum, and yet, there are very few lesson plans or resources available to teachers for these classes specifically. The objectives of this project were to compile a set of lesson plans, lab plans, and tests that would emphasize interactive and investigative learning. The lesson plans were written in such a way that they reflected a certain set of standards, set down by the state of California, and were meant to serve as a bare outline of topics that would be discussed within a week’s worth of …

Enhancement Or Attenuation Of Disease By Deletion Of Genes From Citrus Tristeza Virus, Satyanarayana Tatineni, William O. Dawson

Department of Plant Pathology: Faculty Publications

Stem pitting is a common virus-induced disease of perennial woody plants induced by a range of different viruses. The phenotype results from sporadic areas of the stem in which normal xylem and phloem development is prevented during growth of stems. These alterations interfere with carbohydrate transport, resulting in reduced plant growth and yield. Citrus tristeza virus (CTV), a phloem-limited closterovirus, induces economically important stem-pitting diseases of citrus. CTV has three nonconserved genes (p33, p18, and p13) that are not related to genes of other viruses and that are not required for systemic infection of some species of citrus, which allowed …

The Evolution Of Natural Plant Communities Through Crop Migration And Crop-To-Weed Gene Flow, Meredith G. Schafer

Graduate Theses and Dissertations

With the rising demand for food and biofuels, the efficiency of crop production has become vital to assuring food security. Genetically engineered (GE) crop varieties are broadly thought to be a solution for improving the efficiency of crop production, but concerns regarding their release have heightened as more discoveries have focused on the influence of genetically engineered crops on native and weedy plant species. Risk assessment of GE crop varieties focuses on two main areas: feralization of crop systems and crop-weed hybridization. Risks include naturalization, transgene introgression, and the formation of novel genotypes in natural systems. In this study, roadside …