Digital Commons Network^™

Serum Comp And The Inflammatory Environment Of The Knee Before And After Exercise-Induced Load, Alyssa Evans, Robert D. Hyldahl, Sarah T. Ridge, Sunku Kwon, Ty J. Hopkins, Eric R. Robinson, Matthew K. Seeley

Biomedical Engineering Western Regional Conference

The purpose of this study was to determine how accurately serum COMP represents inflammatory cytokine levels, within the knee, before and after exercise.

Cytokines included: GM-CSF, IFNa2, IL-10, IL-12p40, IL-13, IL-15, IL-1ra, IL1b, IL1a, IL-4, IL-6, IL-8, IP-10, MCP-1, MIP-1a, MIP-1b and IL-17.

Genetic Targeting In Cerebellar Purkinje Cells: An Update., Anna Sługocka, Jan Wiaderkiewicz, Jaroslaw J Barski

Pharmacology and Physiology Faculty Publications

Since the last review paper published in Cerebellum in 2002 [1], there has been a substantial increase in the number of experiments utilizing transgenic manipulations in murine cerebellar Purkinje cells. Most of these approaches were made possible with the use of the Cre/loxP methodology and pcp2/L7 based Cre recombinase expressing transgenic mouse strains. This review aims to summarize all studies which used Purkinje cell specific transgenesis since the first use of mouse strain with Purkinje cell specific Cre expression in 2002.

Mitochondria Mediate Cell Membrane Repair And Contribute To Duchenne Muscular Dystrophy., Maria C Vila, Sree Rayavarapu, Marshall W Hogarth, Jack H Van Der Meulen, Adam Horn, Aurelia Defour, Shin'ichi Takeda, Kristy J. Brown, Yetrib Hathout, Kanneboyina Nagaraju, Jyoti K. Jaiswal

Genomics and Precision Medicine Faculty Publications

Dystrophin deficiency is the genetic basis for Duchenne muscular dystrophy (DMD), but the cellular basis of progressive myofiber death in DMD is not fully understood. Using two dystrophin-deficient mdx mouse models, we find that the mitochondrial dysfunction is among the earliest cellular deficits of mdx muscles. Mitochondria in dystrophic myofibers also respond poorly to sarcolemmal injury. These mitochondrial deficits reduce the ability of dystrophic muscle cell membranes to repair and are associated with a compensatory increase in dysferlin-mediated membrane repair proteins. Dysferlin deficit in mdx mice further compromises myofiber cell membrane repair and enhances the muscle pathology at an asymptomatic …

Gestational Diabetes Mellitus And The Predisposing Factors, Syeda Sadia Fatima, Rehana Rehman, Faiza Alam, Sarosh Madhani, Bushra Chaudhry, Taseer Ahmed Khan

Department of Biological & Biomedical Sciences

Objective: To evaluate the occurrence of gestational diabetes mellitus and its association with demographic and anthropometric variables in pregnant women.
Methods: This cross-sectional study was conducted at the Aga Khan University Hospital, Abbasi Shaheed Hospital and Memon Hospital in Karachi, from February 2014 to December 2015, and comprised pregnant women who were screened by 75-g 2-hour oral glucose tolerance test, (24-28 weeks of gestation) and classified as per the criteria of the International Association of Diabetes and Pregnancy Study Group. Weight, body mass index and serum glycated haemoglobin levels were measured. Women with pre-gestational diabetes were excluded. SPSS 21 was …

Myocardial Relaxation Is Accelerated By Fast Stretch, Not Reduced Afterload, Charles S. Chung, Charles W. Hoopes, Kenneth S. Campbell

Physiology Faculty Publications

Fast relaxation of cross-bridge generated force in the myocardium facilitates efficient diastolic function. Recently published research studying mechanisms that modulate the relaxation rate has focused on molecular factors. Mechanical factors have received less attention since the 1980s when seminal work established the theory that reducing afterload accelerates the relaxation rate. Clinical trials using afterload reducing drugs, partially based on this theory, have thus far failed to improve outcomes for patients with diastolic dysfunction. Therefore, we reevaluated the protocols that suggest reducing afterload accelerates the relaxation rate and identified that myocardial relengthening was a potential confounding factor. We hypothesized that the …

Quality Control By Isoleucyl-Trna Synthetase Of Bacillus Subtilis Is Required For Efficient Sporulation, Elizabeth Kermgard, Zhou Yang, Annika-Marisa Michel, Rachel Simari, Jacqueline Wong, Michael Ibba, Beth A. Lazazzera

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Isoleucyl-tRNA synthetase (IleRS) is an aminoacyl-tRNA synthetase whose essential function is to aminoacylate tRNA^Ile with isoleucine. Like some other aminoacyl-tRNA synthetases, IleRS can mischarge tRNA^Ile and correct this misacylation through a separate post-transfer editing function. To explore the biological significance of this editing function, we created a ileS(T233P) mutant of Bacillus subtilis that allows tRNA^Ile mischarging while retaining wild-type Ile-tRNA^Ile synthesis activity. As seen in other species defective for aminoacylation quality control, the growth rate of the ileS(T233P) strain was not significantly different from wild-type. When the ileS(T233P) strain was assessed for its ability to promote …

Transforming Growth Factor-Β In Thoracic Aortic Aneurysms: Good, Bad, Or Irrelevant?, Alan Daugherty, Zheying Chen, Hisashi Sawada, Debra L. Rateri, Mary B. Sheppard

Physiology Faculty Publications

No abstract provided.

Ms-Read: Quantitative Measurement Of Amino Acid Incorporation, Kyle Mohler, Hans-Rudolph Aerni, Brandon Gassaway, Jiqiang Ling, Michael Ibba, Jesse Rinehart

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Ribosomal protein synthesis results in the genetically programmed incorporation of amino acids into a growing polypeptide chain. Faithful amino acid incorporation that accurately reflects the genetic code is critical to the structure and function of proteins as well as overall proteome integrity. Errors in protein synthesis are generally detrimental to cellular processes yet emerging evidence suggest that proteome diversity generated through mistranslation may be beneficial under certain conditions. Cumulative translational error rates have been determined at the organismal level, however codon specific error rates and the spectrum of misincorporation errors from system to system remain largely unexplored. In particular, until …

Novel Interconnections In Lipid Metabolism Revealed By Overexpression Of Sphingomyelin Synthase-1, Gergana M. Deevska, Patrick P. Dotson Ii, Alexander A. Karakashian, Giorgis Isaac, Mark Wrona, Samuel B. Kelly, Alfred H. Merrill Jr., Mariana N. Nikolova‑Karakashian

Physiology Faculty Publications

This study investigates the consequences of elevating sphingomyelin synthase 1 (SMS1) activity, which generates the main mammalian sphingolipid, sphingomyelin. HepG2 cells stably transfected with SMS1 (HepG2-SMS1) exhibit elevated enzyme activity in vitro and increased sphingomyelin content (mainly C22:0- and C24:0-sphingomyelin) but lower hexosylceramide (Hex-Cer) levels. HepG2-SMS1 cells have fewer triacylglycerols than controls but similar diacylglycerol acyltransferase activity, triacylglycerol secretion, and mitochondrial function. Treatment with 1 mm palmitate increases de novo ceramide synthesis in both cell lines to a similar degree, causing accumulation of C16:0-ceramide (and some C18:0-, C20:0-, and C22:0-ceramides) as well as C16:0- and C18:0-Hex-Cers. In these experiments, the …

Omecamtiv Mecarbil Enhances The Duty Ratio Of Human Β-Cardiac Myosin Resulting In Increased Calcium Sensitivity And Slowed Force Development In Cardiac Muscle, Anja M. Swenson, Wanjian Tang, Cheavar A. Blair, Christopher M. Fetrow, William C. Unrath, Michael J. Previs, Kenneth S. Campbell, Christopher M. Yengo

Physiology Faculty Publications

The small molecule drug omecamtiv mecarbil (OM) specifically targets cardiac muscle myosin and is known to enhance cardiac muscle performance, yet its impact on human cardiac myosin motor function is unclear. We expressed and purified human β-cardiac myosin subfragment 1 (M2β-S1) containing a C-terminal Avi tag. We demonstrate that the maximum actin-activated ATPase activity of M2β-S1 is slowed more than 4-fold in the presence of OM, whereas the actin concentration required for half-maximal ATPase was reduced dramatically (30-fold). We find OM does not change the overall actin affinity. Transient kinetic experiments suggest that there are …

Fröhlich Effect And Delays Of Visual Attention, Nika Adamian, Patrick Cavanagh

Dartmouth Scholarship

In the Fröhlich effect, the initial position of an object that suddenly appears in motion is perceived as being shifted in the direction of its motion. Here we establish that this shift is not an obligatory consequence of motion, but it is driven by focused attention. In Experiment 1 using different cueing conditions, we found that invalid cues produced larger perceptual shifts, although the Fröhlich effect was still present for valid and neutral cues. These results support Müsseler and Aschersleben's (1998) proposal that the Fröhlich effect is the result of the time it takes to shift focal attention to the …

Diaphragm Abnormalities In Patients With End-Stage Heart Failure: Nadph Oxidase Upregulation And Protein Oxidation, Bumsoo Ahn, Philip D. Coblentz, Adam W. Beharry, Nikhil Patel, Andrew R. Judge, Jennifer S. Moylan, Charles W. Hoopes, Mark R. Bonnell, Leonardo F. Ferreira

Physiology Faculty Publications

Patients with heart failure (HF) have diaphragm abnormalities that contribute to disease morbidity and mortality. Studies in animals suggest that reactive oxygen species (ROS) cause diaphragm abnormalities in HF. However, the effects of HF on ROS sources, antioxidant enzymes, and protein oxidation in the diaphragm of humans is unknown. NAD(P)H oxidase, especially the Nox2 isoform, is an important source of ROS in the diaphragm. Our main hypothesis was that diaphragm from patients with HF have heightened Nox2 expression and p47^phox phosphorylation (marker of enzyme activation) that is associated with elevated protein oxidation. We collected diaphragm biopsies from patients with …

Modulating Beta-Cardiac Myosin Function At The Molecular And Tissue Levels, Wanjian Tang, Cheavar A. Blair, Shane D. Walton, András Málnási-Csizmadia, Kenneth S. Campbell, Christopher M. Yengo

Physiology Faculty Publications

Inherited cardiomyopathies are a common form of heart disease that are caused by mutations in sarcomeric proteins with beta cardiac myosin (MYH7) being one of the most frequently affected genes. Since the discovery of the first cardiomyopathy associated mutation in beta-cardiac myosin, a major goal has been to correlate the in vitro myosin motor properties with the contractile performance of cardiac muscle. There has been substantial progress in developing assays to measure the force and velocity properties of purified cardiac muscle myosin but it is still challenging to correlate results from molecular and tissue-level experiments. Mutations that cause …

Predictive Screening Of M1 And M2 Macrophages Reveals The Immunomodulatory Effectiveness Of Post Spinal Cord Injury Azithromycin Treatment, John C. Gensel, Timothy J. Kopper, Bei Zhang, Michael B. Orr, William M. Bailey

Spinal Cord and Brain Injury Research Center Faculty Publications

Spinal cord injury (SCI) triggers a heterogeneous macrophage response that when experimentally polarized toward alternative forms of activation (M2 macrophages) promotes tissue and functional recovery. There are limited pharmacological therapies that can drive this reparative inflammatory state. In the current study, we used in vitrosystems to comprehensively defined markers of macrophages with known pathological (M1) and reparative (M2) properties in SCI. We then used these markers to objectively define the macrophage activation states after SCI in response to delayed azithromycin treatment. Mice were subjected to moderate-severe thoracic contusion SCI. Azithromycin or vehicle was administered beginning 30 minutes post-SCI and …

Genome Editing Of Wnt-1, A Gene Associated With Segmentation, Via Crispr/Cas9 In The Pine Caterpillar Moth, Dendrolimus Punctatus, Huihui Liu, Qun Liu, Xuguo Zhou, Yongping Huang, Zhen Zhang

Entomology Faculty Publications

The pine caterpillar moth, Dendrolimus punctatus, is a devastating forest pest. Genetic manipulation of this insect pest is limited due to the lack of genomic and functional genomic toolsets. Recently, CRISPR/Cas9 technology has been demonstrated to be a promising approach to modify the genome. To investigate gene functions during the embryogenesis, we introduced CRISPR/Cas9 system in D. punctatus to precisely and effectively manipulate gene expressions inmutant embryos. Compared to controls, knocking out of DpWnt-1, a gene well known for its role in the early body planning, led to high embryonic mortality. Among these mutants, 32.9% of the embryos …

Eccentric Resistance Training In Adults With And Without Spinal Cord Injuries, Whitley J. Stone, Sandra L. Stevens, Dana K. Fuller, Jennifer L. Caputo

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 154-165, 2017 The purpose of this study was to examine the effects of active lower body eccentric resistance training (ERT) in individuals with incomplete spinal cord injury (iSCI) and controls (CON). Specifically, the study was designed to determine if those with iSCI adapt similarly to ERT as CON participants as well as the overall safety and efficacy of ERT in this population. This pilot investigation involved the recruitment of persons with iSCI (n = 3) and age- and sex-matched able-bodied CON (n = 3). The 8-week intervention focused on building lower extremity …

The Accuracy Of Pedometers In Measuring Walking Steps On A Treadmill In College Students, Hannah M. Husted, Tamra Llewellyn

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 146-153, 2017. Pedometers are a popular way for people to track if they have reached the recommended 10,000 daily steps. Therefore, the purpose of this study was to determine the accuracy of four brands of pedometers at measuring steps, and to determine if a relationship exists between pedometer cost and accuracy. The hypothesis was that the more expensive brands of pedometers (the Fitbit Charge™ and Omron HJ-303™) would yield more accurate step counts than less expensive brands (the SmartHealth – Walking FIT™ and Sportline™). While wearing all pedometers at once, one male and eleven …

The Effect Of High Intensity Interval Run Training On Cross Sectional Area Of The Vastus Lateralis In Untrained College Students, Rebekah Estes, David Thrush, Amy Malinowski, Meredith Piacentini, Eric Salley, Cassidy Losey, Erik Hayes

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 137-145, 2017 Aerobic cycling has been repeatedly shown to induce hypertrophy in skeletal muscle across a variety of populations, while there has been a lack of investigation into the impact of running upon hypertrophy. An increasingly popular model of aerobic exercise is high-intensity interval training (HIIT); in addition to its positive impact upon cardiovascular health, HIIT may be sufficient for inducing significant muscular hypertrophy. Therefore, the purpose of this investigation was to examine the influence of a high-intensity interval running protocol upon hypertrophy of the vastus lateralis in an untrained, young population. Twelve recreationally …

Effects Of Simulated Altitude On Maximal Oxygen Uptake And Inspiratory Fitness, Nicole Biggs, Benjamin England, Nicole Turcotte, Melissa R. Cook, Alyne L. Williams

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 128-136, 2017 Aerobic exercise at altitude has shown an increase in maximal oxygen uptake. Similar effects have been replicated by way of simulated altitude training, which have influenced various advances in the field of exercise science. Elevation Training Masks© (ETM) claim to stimulate cardiorespiratory fitness improvements similar to training at altitude, however, there is little research to support this claim. The purpose of this study was to research the effect that a hypoxia-inducing mask would have on cardiorespiratory fitness and pulmonary function through the use of a high intensity interval training (HIIT) running program. …

Meeting Usdhhs Physical Activity Guidelines And Health Outcomes, E. Earlynn Lauer, Allen W. Jackson, Scott B. Martin, James R. Morrow Jr.

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 121-127, 2017 Current public health physical activity (PA) guidelines suggest ≥500 METmin/week of PA, with additional benefits beyond 1000 METmin/week (i.e., a dose response). Revised U.S. PA guidelines are scheduled for 2018. The purpose was to relate health markers (blood pressure, percent fat, BMI, blood glucose, cholesterol, and cardiorespiratory fitness) to verify the dose response for PA guidelines revision. 505 non-smoking participants self-reported PA behaviors and completed medical screening. MANCOVA controlling for age and gender determined the relation between health markers and PA. MANCOVA indicated significantly (P<.001) different health markers of percent fat, BMI, glucose, and treadmill time as a function of PA. Post-hoc Helmert contrasts (1] <500 METmin/week vs. ≥500 METmin/week and 2] 500 to <1000 METmin/week vs. ≥1000 METmin/week) indicated that meeting the PA guidelines was associated with better health markers and higher cardiorespiratory fitness. Effect sizes were greater for contrast 1 than for contrast 2, suggesting a plateauing effect. Revised public health guidelines should consider achievement of ≥500 METmin/week to be most important, with additional modest gain thereafter. Revised PA guidelines should stress the importance of achieving 500-1000 METmin/week.

Acute Effects Of 24-H Sleep Deprivation On Salivary Cortisol And Testosterone Concentrations And Testosterone To Cortisol Ratio Following Supplementation With Caffeine Or Placebo, Ciaran K. Mc Donald, Joss Moore, Alan Mc Intyre, Kevin Carmody, Bernard Donne

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 108-120, 2017. Caffeine has become a popular ergogenic aid amongst athletes and usage to improve athletic performance has been well documented. The effect of caffeine on anabolic and catabolic hormones in a sleep-deprived state has had little investigation to date. The purpose of the current study was to investigate the potential of caffeine to offset the effects, if any, of short-term sleep deprivation and exercise on an athlete’s testosterone and cortisol concentrations via salivary technique. Eleven competitive male athletes volunteered to be part of this prospective double-blinded study. Three test days were scheduled for …

The Effects Of A 10-Day Altitude Training Camp At 1828 Meters On Varsity Cross-Country Runners, Sebastian R. Diebel, Ian Newhouse, David S. Thompson, Vineet Bk Johnson

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 97-107, 2017. Altitude training has been shown to alter blood lactate (BL) levels due to alterations resulting from acclimatization. This study aims to estimate the impact of altitude training on BL changes immediately following an incremental treadmill test and during recovery before and after 10-day altitude training at approximately 1828 meters. Eight varsity cross-country runners performed an incremental treadmill test (ITT), pre and post-altitude training. Resting and post-warm-up BL values were recorded. During ITT, heart rate (HR), oxygen saturation (SpO2), and time to exhaustion were monitored. BL was also measured post-ITT at 0, 2, …

Validity Of The Bottle Buoyancy Model For Body Fat Determination, Cherie Pettitt, Kalvin Danzl, Lauren M. Glorvigen, Cody J. Strom, Thomas A. Inkrott, Robert W. Pettitt

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 87-96, 2017. We investigated a modification of the bottle buoyancy (BB) method in comparison to single frequency, bioelectric impedance analysis (BIA) as a valid noninvasive method of percent body fat (%BF) determination. Twenty-eight participants (15 men, 13 women), in counterbalanced-order, completed the BB, BIA, and computerized hydrostatic densitometry (HD) methods. We elected to modify the BB method using a 12.15 L container with participants hugging the container in an upright position. Consistency measures of intraclass correlation coefficient (ICC), typical error (TE), coefficient of variation (CV) and total error of measurement (TEM) are reported. Our …

Borg Scale Is Valid For Ratings Of Perceived Exertion For Individuals With Parkinson’S Disease, Amanda Penko

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 76-86, 2017. Parkinson’s disease is a neurodegenerative disease that has traditionally been treated with anti-parkinsonian medication. There is increasing evidence that exercise is beneficial to those with PD, therefore, it is necessary to validate a measure of exertion that can be implemented across exercise settings that may not have the capability to actively monitor heart rate. The aim of this project was to determine the validity of the Borg RPE scale in individuals with PD undergoing a maximal progressive cycling exercise test. Thirty-eight males and females (58.5 ± 8.1 yrs) with a clinical diagnosis …

Assessing Energy Level As A Marker Of Aerobic Exercise Readiness: A Pilot Investigation, Kelley Strohacker, William R. Boyer, Kayla N. Smitherman, Emily Cornelius, Daniel Fazzino

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 62-75, 2017. Energy ratings have been used as a marker of exercise readiness (i.e. pre-exercise physical/mental state indicating ability to perform) within flexible nonlinear periodization (FNLP)-based resistance training interventions. However, empirical data is lacking regarding the utility of this approach for aerobic exercise. The purpose of this study was to examine the ability of pre-exercise energy level to predict affective and behavioral responses to prescribed aerobic exercise. Participants consisted of 19 women and 8 men (N=27, age=20±4 years, estimated maximal oxygen uptake=37±6). Participants performed two 30-min bouts of treadmill exercise under an imposed moderate …

The Acute Effects Of Static Stretching Compared To Dynamic Stretching With And Without An Active Warm Up On Anaerobic Performance, Bradley J. Kendall

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 53-61, 2017. The Wingate Anaerobic Test (WAnT) has been used in many studies to determine anaerobic performance. However, there has been poor reporting of warm-up protocols and limited consistency between warm-up methods that have been used. With the WAnT being such a commonly-used test, consistency in warm-up methods is essential in order to compare results across studies. Therefore, this study was designed to compare how static stretching, dynamic stretching, and an active warm-up affect WAnT performance. Ten recreationally active participants (5 males, 5 females) with a mean (SD) age of 23.3 (0.7) volunteered for …

The Relationship Between Sports Specialization And Mental Toughness, Courtney Buhrow, Jacob Digmann, Jennifer J. Waldron

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 44-52, 2017. The pros and cons of early sport specialization compared to diversification have been examined in many research studies. The purpose of this study was to determine (a) the relationship between mental toughness and age of specialization in sport, and (b) differences in mental toughness based on early specialization of sport and gender. College athletes (N = 102) completed surveys about specialization and mental toughness, including MeBTough. The mean age of specialization was 13.45 (± 4.47). Results showed no significant difference in mental toughness of those who specialized early and those who did …

The Effects Of An Elevation Training Mask On Vo2max Of Male Reserve Officers Training Corps Cadets, Brian G. Warren, Frank Spaniol, Randy Bonnette

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 37-43, 2017. The purpose of this study was to investigate the effects of an elevation training mask (ETM) on the VO2max of male Reserve Officers Training Corps (ROTC) cadets. Fourteen male ROTC cadets (age 20.00 ± 1.8 yrs, height 174.35 cm ± 3.1 cm, weight 76.75 kg ± 11.09 kg, body fat 13.88% ± 4.62%) participated in this study to determine if an ETM would cause a significant increase VO2max. After the familiarization period, the test subjects were randomly assigned to either the control or experimental group, respectively. The training period lasted seven weeks …

The Effect Of Military Load Carriage On Postural Sway, Forward Trunk Lean, And Pelvic Girdle Motion, Eileen M. Strube, Andrea Sumner, Roger O. Kollock Jr, Kenneth E. Games, Marie A. Lackamp, Masahiro Mizutani, Joellen M. Sefton

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 25-36, 2017. Musculoskeletal injuries are a common occurrence in military service members. It is believed that the load carried by the service member impedes stability and alters back and pelvis kinematics, increasing their susceptibility to musculoskeletal injuries, specifically in the lower extremities. The purpose of this study was to examine the effects of two different loads on postural sway, forward trunk lean, and pelvic girdle motion in United States Army Cadets. Twenty male Army Reserve Officers’ Training Corps Cadets participated in this study. Each participant performed the Modified Clinical Testing of Sensory Interaction (mCTSIB) …

Sport Commitment Among Adult Recreational Soccer Players: Test Of An Expanded Model, Amanda L. Frayeh, Beth Lewis

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 4-24, 2017. Adult sport leagues provide opportunities for physical activity (PA), but little is known about adults’ commitment to recreational sports. We proposed two expanded versions of Scanlan and colleagues’ sport commitment model (SCM) to explore psychosocial factors related to adults’ participation in these leagues. Model 1 proposed athletic identity and social cohesion as additional predictors of commitment. Previous research found these variables to be positively related to commitment, but had not systematically examined them within the context of the SCM. We also explored participation in moderate-to-vigorous physical activity (MVPA) as a behavioral manifestation …