Digital Commons Network^™

Macrophages Are Necessary For Epimorphic Regeneration In African Spiny Mice, Jennifer Simkin, Thomas R. Gawriluk, John C. Gensel, Ashley W. Seifert

Biology Faculty Publications

How the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly comparing inflammatory cell activation in a 4 mm ear injury during regeneration (Acomys cahirinus) and scarring (Mus musculus), we found that both species exhibited an acute inflammatory response, with scarring characterized by stronger myeloperoxidase activity. In contrast, ROS production was stronger and more persistent during regeneration. By depleting macrophages during injury, we demonstrate …

Diverse Tissue And Organ Systems Differs Following Exposure To Secondhand Cigarette Smoke, Jason Gassman, Dr. Paul R. Reynolds

Journal of Undergraduate Research

The receptor for advanced glycation end-products (RAGE) is a pattern recognition receptor expressed in many cell types, including smooth muscle cells, fibroblasts, macrophages, and epithelial cells (1). RAGE is capable of binding a variety of endogenous ligands, included advanced glycation end-products (AGEs), S100/calgranulins, amyloid-beta peptides, and HMGB1 (2). RAGE signaling has been identified as a primary mediator in a variety of feed-forward inflammatory pathways and, as such, has been implicated in a variety of chronic conditions of inflammation such as chronic obstructive pulmonary disease (COPD), pancreatitis, and diabetic cardiomyopathy (3-5).

Changes In Running Economy, Respiratory Exchange Ratio And Vo2max In Runners Following A 10-Day Altitude Training Camp, Sebastian R. Diebel, Ian Newhouse, David S. Thompson, Vineet B.K. Johnson

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 629-639, 2017. Running economy (RE) and VO₂max are important predictors of endurance performance for elite and semi-elite endurance athletes, with RE being an appropriate predictor in a homogenous running population. Altitude training has been observed to change RE (mL.kg^-1.min^-1), and VO₂max due to alterations resulting from acclimatization. This study tracked changes in RE and VO₂max before and after a 10-day altitude training camp at 1828 meters. VO₂max_, RE expressed calorically, and respiratory exchange ratio (RER), were measured below anaerobic threshold (AT) …

Localization Of Cell Bodies Of The Stellate And Superior Cervical Ganglia In Relation To Innervation Of Cardiac Muscle And Vasculature Of The Brain, Alistair Hilton, Jonathan J. Wisco

Journal of Undergraduate Research

Introduction: Arrhythmia is a serious heart defect that effects 14 million people in the United States.i When the impulse rhythm becomes irregular or the frequency of atrial and ventricular beats are different it is called arrhythmia.ii The most serious effects of arrhythmia include sudden cardiac arrest and stroke.iii Sympathetic nerve activity from the stellate ganglia increases prior to arrhythmia events leading to sudden cardiac death.iv About 383,000 cases of cardiac arrest are recorded annually in the United States alone.v Current treatment for arrhythmia consists of ablation or intentional, permanent scarring of the heart tissue, rather than a more targeted block …

King-Devick Performance Following Moderate And High Exercise Intensity Bouts., Billymo Rist, Adrian Cohen, Alan J. Pearce

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 619-628, 2017. The King-Devick (K-D) test is a concise, noninvasive assessment of oculomotor and cognitive function that has been shown to detect sub-optimal brain performance following sports head trauma. Used in a number of sports as a sideline concussion assessment tool, the K-D test can be administered by non-medical personnel. However, the issue regarding the effect of exercise on K-D performance has not been fully explored. Using a randomized crossover design, this study aimed to compare the effect of two intensities of exercise on K-D performance. Twenty males (21.2 ± 1.9 years) completed the …

Mood And Performance Anxiety In High School Basketball Players: A Pilot Study, Steffen J. Hoover, Rachel K. Winter, Holly Mccutchan, Christina C. Beaudoin, Lawrence W. Judge, Lani M. Jones, Brianna Leitzlar, Donald L. Hoover

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 604-618, 2017. Participation in competitive sport may impact psychological measures, such as mood and performance anxiety, which in turn may impact enjoyment, adherence, continued participation, and so on. This study assessed the feasibility – in terms of process, resources, management, and potential scientific value– of measuring the effect of varying competitive challenges upon the mood and performance anxiety measures of high school athletes. The participants (n=12) consisted of the boys’ varsity basketball team at a high school in a rural Midwestern community. Participants completed the Profile of Mood States (POMS) to assess mood and …

The Effect Of Incline On Caloric Expenditure Measured By A Wrist-Worn Commercial Activity Monitor, Kaitlin M. Bickel

Senior Honors Projects, 2010-2019

Purpose Physical activity monitors have become popular among the general public to monitor steps, floors climbed, active minutes, and energy expenditure (EE). While there is evidence to support that these devices are accurate in counting steps, there is limited and inconclusive research regarding how accurate they are in measuring EE. This study aimed to test the accuracy of a newer commercial physical activity monitor, the Fitbit Charge (FC), in reporting EE compared to a research-grade accelerometer (GT3X), and indirect calorimetry (IC) while walking on a treadmill with and without incline.

Methods 30 subjects (22 female and 8 male) walked on …

Mathematical Modeling And Expression Of Heart Rate Deflection Point Using Heart Rate And Oxygen Consumption, Kayla M. Baker, David H. Fukuda Ph.D., David Church, Michael B. La Monica, Kyle S. Beyer, Jay R. Hoffman, Jeffrey R. Stout Ph.D.

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 592-603, 2017. Heart rate deflection point (HRDP) can be determined through different mathematical-modeling procedures, such as bi-segmental linear regression (2SEG) or maximal distance model (Dmax). The purpose was to compare heart rate (HR) and oxygen consumption (VO₂) at HRDP when using 2SEG and Dmax, and to examine their relationships with respiratory compensation point (RCP) and running performance. Nineteen participants completed a graded exercise test (GXT), to determine HRDP and RCP, and a 5km treadmill time trial (5K_time). No differences were found in HR or VO₂ when comparing HRDP_{2SEG …}

The Effects Of Work-To-Rest Ratios On Torque, Electromyographic, And Mechanomyographic Responses To Fatiguing Workbouts, Ethan C. Hill, Terry J. Housh, Cory M. Smith, Kristen C. Cochrane, Nathaniel D.M. Jenkins, Richard J. Schmidt, Glen O. Johnson

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 580-591, 2017. The purpose of the present study was to examine the effects of 2 different work-to-rest ratios, but the same mean load, cycle time, and total duration of the exercise bout, on maximal voluntary isometric contraction torque and neuromuscular responses to fatiguing, intermittent, submaximal, isometric, forearm flexion muscle actions. Ten men performed 2 fatiguing protocols with different work-to-rest ratios (4 s contraction, 4 s rest vs. 4 s contraction, alternating 6 and 2 s rest) that consisted of 50 intermittent, submaximal (65% of maximal voluntary isometric contraction), isometric, forearm flexion muscle actions. Electromyographic …

Hibernator And Non-Hibernator Responses To Acute Changes In Water Intake, Sydni S. Andruskiewicz

All College Thesis Program, 2016-2019

Hibernating animals undergo dramatic changes in metabolic rates during torpor. One of most notable changes in these animals is the ability to maintain blood pressure and perfuse certain organs. Consequentially, blood perfusion to the kidneys is greatly decreased and the ability to concentrate urine halts. However, about once a week, torpor is interrupted and the animal becomes active to rewarm itself about once a week. This activity induces rapid regeneration of the extracellular osmotic gradient of the kidney, and allows urine to be concentrated. Nonetheless, regaining the extracellular osmotic gradient creates a potentially fatal consequence to the kidney cells. To …

Optimal Nutrition For Endurance Exercise: A Systematic Review, Sarah E. Andrus Ms, Bruce W. Andrus Md Ms

Dartmouth Scholarship

Introduction

As fatigue in endurance events correlates with depletion of muscle glycogen, the traditional approach to nutritional support has been carbohydrate loading. However, there has been recent interest in improving athletic endurance performance by novel diets in the days to weeks prior to endurance events, the pre-event meal, and during exercise.

Methods

We searched PubMed and SCOPUS for randomized trials published from 1992-2017 with a primary endpoint of endurance performance. We identified 407 citations which were examined against our inclusion criteria of randomization or crossover allocation to diet and for which a primary outcome was endurance performance.

Results

Twenty full …

Physiological Characteristics Of Projected Starters And Non-Starters In The Field Positions From A Division I Women’S Soccer Team, Fabrice G. Risso, Farzad Jalilvand, Ashley J. Orjalo, Matthew R. Moreno, Deshaun L. Davis, Samantha A. Birmingham-Babauta, John J. Stokes, Alyssa A. Stage, Tricia M. Liu, Dominic V. Giuliano, Adrina Lazar, Robert G. Lockie

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 568-579, 2017. NCAA soccer features different substitution rules compared to FIFA-sanctioned matches, with a greater availability of players who can enter the game. This could influence the physiological characteristics of the field position starters (ST) and non-starters (NST) within a collegiate women’s team, which has not been previously analyzed. Thus, 22 field players from the same Division I women’s soccer squad completed: vertical and standing broad jumps; 30-meter (m) sprint (0-5, 0-10, 0-30 m intervals); pro-agility and 60-yard shuttle; and the Yo-Yo Intermittent Recovery Test Level 1. Players were defined into ST (n=10) and …

The Effects Of Coffee Ingestion On The Acute Testosterone Response To Exercise, Taylor Landry

Masters Theses, 2010-2019

Abstract

This study investigated the effects of coffee ingestion (COF) on serum testosterone responses to exercise in recreationally weight-trained males. Subjects ingested either 12 ounces of 6mg/kg caffeinated coffee (COF), decaffeinated coffee (DEC), or water (PLA) one hour prior to exercise in a randomized, within-subject, crossover design. The exercise session consisted of 21 minutes of high intensity interval cycling (alternating intensities corresponding to two minutes at power outputs associated with 2.0 mmol/L lactate and 4.0 mmol/L lactate) followed by resistance exercise (7 exercises, 3 sets of 10 repetitions, 65% 1RM, 1-minute rest periods). Subjects also completed repetitions to fatigue tests …

The Effects Of Caffeine Ingestion On The Hemostatic Response To Simulated Firefighting Activities, Sierra D. Wassell

Masters Theses, 2010-2019

Sudden cardiac events are the leading cause of death among firefighters, and it is acknowledged that most ischemic events are due to an occlusive thrombus formation. In addition, due to the demanding job requirements and shift work, firefighters commonly overuse caffeine. The purpose of this study was to examine the potential effects of caffeine on the hemostatic response to simulated firefighting activity. Twelve healthy male firefighters (age, 31.3 ± 5.4 yrs; weight, 94.2 ± 13.1 kg; BMI, 28.7 ± 2.9 kg·m^-2) participated in this study, wearing full personal protective equipment (PPE) and self-contained breathing apparatus (SCBA). Subjects completed …

The Effect Of Ambient Temperature On Recovery Of Surgical Stress In Sprague-Dawley Rats, Romie D. Powell

Masters Theses, 2010-2019

Laboratory animals are housed at ambient temperatures ranging from 20°C – 26°C per recommended guidelines. Rats in particular typically prefer ambient temperatures (Tamb) of ~27°C (Brown et al, 2011). When rats undergo surgical instrumentation for experimental use, they often recover at normal room temperature (~21°C). While this is comfortable to those maintaining them, it may lead to a cold thermal stress for the rats. It is hypothesized that housing rats at ambient temperatures away from their preferred Tamb could lead to a thermal stress, which adversely affects surgical recovery. To address this, rats (220g-350g) were surgically instrumented with a radiotelemetry …

Effects Of 12 Weeks Of Physical Training On Body Composition And Physical Fitness In Military Recruits, Leandra C B Campos, Fabio A D Campos, Thiago A R Bezerra, Idico L. Pellegrinotti

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 560-567, 2017. The aim of this study was to analyze the morphological and functional changes in military recruits after twelve weeks of physical training. The sample consisted of 130 volunteer recruits aged 18-19 years. Anthropometric variables were assessed (body weight, height, body mass index, arm, waist, and hip circumference, waist/hip ratio, skinfold thickness of the triceps, suprailiac, and abdomen, and body fat percentage) and the sit-up test (1 minute), push-up test, and 12-minute running test. The physical training protocol involved running workouts, strength, agility, and flexibility. Descriptive statistical procedures were performed with mean, standard …

The Effect Of Betaine On Nitrate And Cardiovascular Response To Exercise, J. Luke Pryor, Stephen A. Wolf, Gary Sforzo, Tom Swensen

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 550-559, 2017. Betaine (BT) supplementation improves selected markers of physical performance, however, the mechanism(s) by which this change occurs remains largely unknown. Some speculate that BT may increase circulating nitrate concentrations, improving physical performance by augmentation of endothelial nitric oxide production. The purpose of this study was to investigate the effects of acute BT supplementation and exercise on plasma nitrate levels and related cardiovascular response (CVR). Placebo and BT trials were administered in a cross-over, randomized, double-blind, and counterbalanced fashion. Ten healthy college-aged volunteers consumed either a 250 ml placebo (carbohydrate-electrolyte beverage, CHO) or …

Correlation Between The Functional Movement Screen And Hip Mobility In Ncaa Division Ii Athletes, Matt T. Jenkins, Robert Gustitus, Mike Iosia, Taz Kicklighter, Yuichi Sasaki

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 541-549, 2017. The Functional Movement Screen (FMS) is a series of seven exercises designed to assess athletes prior to competitive training to determine dysfunctional movement patterns. The exercises include the deep squat (DS), hurdle step (HS), inline lunge (IL), shoulder mobility (SM), active straight leg raise (ASL), pushup (PU), and rotary stability (RS), all of which comprise the composite score (FMS CS). The purpose of this study was to determine correlations between the FMS screens and hip mobility. The specific hip range-of-motion exercises used were bilateral internal/external rotation (IRR, IRL ERL, ERR), and flexion/extension …

Cortisol And Alpha-Amylase Changes During An Ultra-Running Event, Whitney P. Deneen, Alexis Jones

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 531-540, 2017. Elevated stress hormone concentrations can positively affect an athlete’s overall performance during a competition, and in many cases, are necessary to be able to perform exercise. During extreme exercise, the body’s ability to utilize energy efficiently can affect an athlete’s performance. Elevated hormonal concentrations can have many benefits in regards to an athlete’s overall performance during a competition. The purpose of this study was to examine the effects of long distance running, such as seen during an ultra-running event (distances beyond 26.2 miles), on the activity of the hypothalamic-pituitary-adrenocortical (HPA) axis production …

Sleep-Hygiene Education Improves Sleep Indices In Elite Female Athletes, Shannon O'Donnell, Matthew W. Driller

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 522-530, 2017. The importance of sleep in providing psychophysiological recovery in elite athletes is often overlooked. In other populations (eg shift workers and adolescent students), sleep hygiene education may serve to acutely improve sleep indices. However, this is yet to be examined in an elite athlete setting. Therefore, the aim of the current study was to evaluate the effect of a sleep hygiene education session on sleep indices in elite athletes. The study involved 26 elite female netball athletes performing one week of baseline sleep monitoring (PRE), followed by a sleep hygiene education …

Differences Between The Grab Start And Track Start In Collegiate Swimmers, Alexander S. Dassoff, Naomi R. Forward, Charlie P. Katica

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 515-521, 2017. The purpose of this investigation was to determine which foot stance, the track start (TS) or the grab start (GS), is most beneficial for competitive swimmers. Seven male and eight female collegiate swimmers participated in this study. The average participant age was 20.1 ± 1.13 years while the average years of competitive swimming experience was 10.8 ± 3.36 years. Participants performed three TS and three GS stances consecutively. Distance markers were placed on the side of the pool to determine where each swimmer entered the water. Video analysis was used to determine …

Effects Of Nasal Or Oral Breathing On Anaerobic Power Output And Metabolic Responses, Christine Recinto, Theodore Efthemeou, P. Tony Boffelli, James W. Navalta

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 506-514, 2017. Nasal and oral exclusive breathing modes have benefits and drawbacks during submaximal exercise. It is unknown whether these responses would extend to anaerobic work performed at high intensity. Nine individuals (males N = 7, females N = 2) performed a standard Wingate Anaerobic cycle test on a cycle ergometer under nose (N) and mouth (M) only respiratory conditions, performed in a counterbalanced order. A 2 (condition: nose, mouth) x 6 (time: 0-5 sec, 5-10 sec, 10-15 sec, 15-20 sec, 20-25 sec, 25-30 sec) repeated measures ANOVA was used to analyze the data …

Rollers Versus Trainers: 10-Km Time Trial, Wayland Tseh, Tate Devlin, Taylor Milleson, T V. Barreira

International Journal of Exercise Science

International Journal of Exercise Science 10(1): 497-505, 2017. The primary aim of this investigation was to determine which cycling training device, Rollers or Trainers, was most effective in improving 10-km time trial. Eight male and 6 female volunteers (N = 14; age = 23.6 ± 4.6 yrs; height = 172.7 ± 9.9 cm; body mass = 68.4 ± 10.4 kg; % body fat = 16.9 ± 7.7; VO_2max = 61.0 ± 9.4 ml∙kg^-1∙min^-1) provided informed consent prior to participation. Participants performed a10-km time trial at baseline and were then randomly assigned into one of three …

Physical Activity And Physical Fitness In Pediatric Obesity: What Are The First Steps For Clinicians? Expert Conclusion From The 2016 Ecog Workshop, Grace O´Malley, Susanne Ring-Dimitriou, Paulina Nowicka, Andrea Vania, Marie-Laure Frelut, Nathalie Farpour-Lambert, Daniel Weghuber, David Thivel

International Journal of Exercise Science

International Journal of Exercise Science 10(4): 487-496, 2017. One of the main European Childhood Obesity Group mission is to assist healthcare workers in delivering evidence-based assessment and treatment of childhood obesity. Every year the ECOG annual work shop includes working groups whose objective is to highlight the major concerns faced by clinicians and practitioners who work in the field of pediatric obesity. This year, a working group was devoted to the assessment of physical activity and physical fitness in this population. The present commentary attempts to summarize the main themes identified by practitioners during these workshops in order to provide …

Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman

Master's Theses

Lower body positive pressure (LBPP) treadmills are growing in popularity for rehabilitative use, as the benefits of exercising at partially supported body weight may induce faster recovery. It is unknown if there are certain practices that increase exercise intensity while maintaining positive effects of LBPP. Adding ankle weights when walking or running could increase intensity of rehabilitation programs while maintaining the comfort of supported body weight. PURPOSE: To measure metabolic response (VO2, RER, HR, Caloric expenditure), RPE, and lower limb electromyography (EMG) amplitudes of LBPP treadmill walking and running with and without ankle weights. METHODS: Sixteen participants (Age: 21.94 ± …

The Effects Of Quadmill Training On Balance: An Intervention Study 2017, Alexander J. Generali

Master's Theses

The purpose of this study was to observe and compare the effects of two separate training interventions using the QuadmillTM to a control group on balance in college-aged individuals. It was hypothesized that both experimental groups (I1 and I2) would experience improvements in balance over the intervention whereas the control group would remain at baseline. It was secondarily hypothesized that I2 would experience greater improvements than I1 in balance due to the nature of the exercise protocol. Data was collected using three systems; Balance Tracking Systems, Star Excursion Balance Test, and the Biodex Balance system. A two-way mixed methods ANOVA …

The Effect Of Blood Flow Restriction Training With The Quadmill On Peak Isometric Knee Extensor Strength 2017, Brandon Schrom

Master's Theses

Traditional training methodologies that improve muscular strength use loads as low as 75% of a person’s one-repetition maximum and as high as 110% of a person’s one repetition maximum. With these high loads comes a greater risk for injury. Blood flow restriction (BFR) training is a potential solution to this problem. BFR training originated in Japan, where it was called Kaatsu. With this method of training, a trainee ties a tourniquet around the proximal end of a limb to reduce blood flow to and from the limb’s muscles. The purpose of this study was to determine whether three-weeks of BFR …

Imposed Versus Affect-Based Resistance Training Intensities On Adherence, Session Affect, Session Perceived Exertion, And Intrinsic Motivation Measures During A Six-Week Program In Novice Female Lifters 2017, Ryan Joseph Brennan

Master's Theses

The American College of Sports Medicine (ACSM) recommends that individuals perform resistance training exercises at a specific intensity based on external load (percentage of one repetition maximum; %1RM). However, only 29.6% of adults in 2013 reported strength training two or more times per week. Furthermore, individuals lifting at recommended percentages vary in their pleasure and displeasure. Self-selected exercise seems to promote positive affective responses, in part, due to the perceived autonomy. The effects of regulating exercise intensity using affect as opposed to imposed intensities as a means for improved fitness, promoting exercise behavior, and enhancing other psychological outcomes have yet …

Chemogenetic Stimulation Of Electrically Coupled Midbrain Gaba Neurons In Alcohol Reward And Dependence, Stephanie Suzette Pistorius

Theses and Dissertations

The prevailing view is that enhancement of dopamine (DA) transmission in the mesolimbic system leads to the rewarding properties of alcohol. The mesolimbic DA system, which plays an important role in regulating reward and addiction, consists of DA neurons in the midbrain ventral tegmental area (VTA) that innervate the nucleus accumbens (NAc). It is believed that VTA DA neurons are inhibited by local gamma-aminobutyric acid (GABA) interneurons that express connexin-36 (Cx36) gap junctions (GJs). We have previously demonstrated that blocking Cx36 GJs suppresses electrical coupling between VTA GABA neurons and reduces ethanol intoxication and consumption suggesting that electrical coupling between …

Enhancing Cell Therapy For Ischemic Cardiomyopathy., Michael J. Book

Electronic Theses and Dissertations

Cardiac cell therapy using cardiac mesenchymal cells (CMC) significantly reduces ventricular dysfunction in patients with ischemic cardiomyopathy. Despite the improvement in function, a modest number of CMCs survive in the heart post-transplantation. In this study, we sought to improve the survival and retention of transplanted CMCs to prolong the therapeutic benefits afforded by cardiac cell therapy. To do this, we targeted the enzyme telomerase (TERT), known to be active in some highly proliferating cells (e.g. germ, stem). TERT is responsible for preventing telomere attrition, thereby allowing continued proliferation. TERT has also been shown to be protective, improve cell migration and …