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Cardio-Renal Mechanisms Of Fructose-Induced Salt-Sensitive Hypertension, Peter Eric Levanovich

Wayne State University Dissertations

Dietary consumption of fructose facilitates increased intestinal fluid absorption and renal sodium reabsorption, thereby increasing fluid retention. The net result of this is a sustained increased in extracellular fluid volume that leads to states of hypervolemia and subsequent hypertension. Simultaneously, arterial pressure is being elevated by increased autonomic drive stemming from the sympathetic nervous system and various other endovascular proteins that induce vasoconstriction. Under these conditions, the addition of high dietary sodium promotes hypertension prior to the development of significant metabolic disturbances; the subtlety of which may go unnoticed by patients for prolonged periods. While much is understood regarding the …

Comparing Interrelated Stress Measures To Differentiate Acute And Chronic Stress In Urban Freshwater Turtles, Katherine Caldwell

Honors Program Theses

As urban areas grow in size and number, there are increasing impacts on the wildlife. While some species adapt, others cannot thrive in these conditions. Stress is the biological response to a stimulus that threatens homeostasis, which can be used to understand the condition of an individual. While acute stress is beneficial for survival, chronic stress can lead to deleterious impacts. A majority of turtles can be classified as declining in number or endangered, yet there is little understanding of their physiological stress response. This study examined how various stress measures—including corticosterone (CORT) in plasma, nails and leeches, metabolic measures, …

Contextualizing The Acute Responses To Arm Cycling, Nicolas Clark

Electronic Theses and Dissertations, 2020-2023

The prescription of aerobic exercise modalities engaging the arm musculature have the potential to extend the reach of physical activity and promote cardiorespiratory fitness in individuals with lower body impairment due to excessive body mass, and among individuals seeking to complement and diversify standard leg training. This study compared the acute cardiopulmonary responses obtained during arm cycling and leg cycling performed at different intensities among lean and average (LA) and overfat and obese individuals (OFO). Participants were 37 young and relatively healthy adults. They were tested for mode-specific peak power output and work rate at ventilatory threshold during two randomized …

Best Practices For Clinical Evaluation Of Sacroiliac Joint Pain: An Evidence-To-Practice Review, Nicolette A. Harris, Adriana Peña, Sofia Núñez Rivera

Clinical Practice in Athletic Training

Sacroiliac joint (SIJ) pain serves as an under-recognized source of chronic low back pain. Improvement in the accuracy of a clinical SIJ pain diagnosis lends a higher likelihood of appropriate treatment measures, better patient outcomes and decreased out-of-pocket costs. Therefore, the overall purpose of this evidence to practice review was to highlight the main points of a systematic review on the clinical diagnosis of SIJ pain. Searches of five electronic databases revealed 758 studies, nonetheless only six studies met final inclusion criteria. Studies included were assessed by the authors for methodological quality using the Quality Assessment of Diagnostic Accuracy Studies …

Disablement Model Case Study: Running With Postural Orthostatic Tachycardia Syndrome, Jordan Fenney, Wendy Reitz, Hayley M. Ericksen

Clinical Practice in Athletic Training

A 17-year-old high school female cross-country runner and basketball player presented with syncope following long-endurance exercise. The syncope episodes started when the patient was 13 years old during a basketball game. After the first episode, the patient fainted every time she crossed the finish line of a cross-country meet. Her symptoms included increased heart rate, shortness of breath, and paresthesia in her hands and legs during exercise. The patient also experienced some dizziness when quickly sitting or standing during activities of daily living. The patient was first misdiagnosed with exercise induced asthma and prescribed a rescue inhaler to take prior …

Athletic Trainers’ Perceived Readiness To Recognize Mental Health Symptoms In Student Athletes, K M. Sumrall, H Salisbury

Clinical Practice in Athletic Training

Context: Athletic trainers (AT) may report a lack of confidence in decision-making as they transition to professional practice. There is limited research on how prepared ATs feel managing mental health conditions in athletes. Objective: The purpose of this study was to explore the perceived readiness and confidence of master’s AT students, transitioning to professional practice, to recognize mental health symptoms and provide appropriate referral strategies. Design: Quantitative, descriptive study. Setting: Texas master’s AT programs. Patients or Other Participants: Non-probability consecutive sampling method. The target population was 2nd-year master’s AT students in Texas who were eligible to graduate and sit for …

Hiring Practices Among Ncaa Division I Head Athletic Trainers, S A. Cage, Z K. Winkelmann, B J. Warner, C Tuell, D M. Gallegos

Clinical Practice in Athletic Training

Context: Limited empirical evidence exists on how athletic trainers (ATs) are hired by NCAA institutions. Current legislation protects many demographics from discrimination during hiring, but these laws may not be enforced by institutions. Objective: The purpose of this study was to describe potential factors that might influence the hiring practices of NCAA Division I Head Athletic Trainers. Design: Cross-sectional design. Setting: Electronic survey distributed to certified athletic trainers. Participants: A web-based survey (Qualtrics) that was distributed to by email to 329 NCAA Division I Head Athletic Trainers. A total of 114 ATs completed the survey (response and completion rate=34.7%). Interventions: …

Nonsurgical Management Of Unilateral, Nondisplaced Lateral Malleolus Fracture In 17-Year-Old Outside Linebacker, K Tallman, R Bautista, B Spencer

Clinical Practice in Athletic Training

Background: Ankle fractures, specifically unilateral fractures, are among the most common injuries encountered by orthopedic surgeons. Ankle fractures have an incidence of 187 out of 100,000 individuals. Current treatment options for ankle fractures are dependent on stability of the ankle mortise, determined through number of fracture sites and ligamentous integrity. The management of the fracture scan either be surgical or nonsurgical, the majority being surgical. In this case report, a 17-year-oldmaleoutside linebacker sustained a left lateral ankle injury during a regular season football game. As he attempted to make a tackle, his left foot got caught in the turf, he …

Stress, Burnout, And Depression Among African Immigrant Direct Support Professionals Working With Adults With Intellectual Disabilities, Mary A. Onyejose

Walden Dissertations and Doctoral Studies

AbstractStress and burnout are recognized problems among staff working with individuals with intellectual and developmental disabilities (IDDs). However, information on stress and burnout in African immigrant direct support professionals (DSPs) is lacking. The purpose of this correlational mediation study was to investigate whether burnout mediates the relationship between stress and depression among African immigrants working with adults with IDDs. Job stress theory and burnout theory served as the theoretical framework for the study. Overall, 111 DSPs completed self-report questionnaires: the Maslach Burnout Inventory, Parker and DeCotiis’ Job Stress Scale, and the Beck Depression Inventory. Results revealed that stress significantly predicted …

The Tumor Suppressor Par-4 Regulates Hypertrophic Obesity, Nathalia Araujo

Theses and Dissertations--Toxicology and Cancer Biology

Prostate Apoptosis Response-4 (Par-4) is a tumor suppressor ubiquitously expressed in all tissues and able to selectively induce apoptosis in cancer cells. Although well established in the context of cancer, relatively little is known about the function of Par-4 in the healthy and non-tumorigenic context. Observations from our lab showed that Par-4 knockout mouse lines were obese and displayed adipocyte hypertrophy under a normal chow diet when compared to Par-4 wild-type mice. These Par-4 knockout mice exhibited hepatic steatosis and hyperinsulinemia as secondary consequences of obesity. Par-4 knockout mice displayed increased intestinal dietary fat absorption and its subsequent storage in …

Effect Of Imagery And Heart Rate Variability Biofeedback Training On Selected Soccer Skills Performance, Sara Majlesi

Student Works (2020-2029)

The present study aimed to evaluate the influence of PETTLEP video imagery (Physical, Environment, Task, Timing, Learning, Emotion and Perspective), heart rate variability biofeedback (HRVB), and concurrent intervention of both on selected soccer skill performances. The objective of this study was to evaluate the effect of PETTLEP video imagery, HRVB and concurrent intervention on soccer players' reactive motor skill test (RMST), sprint time, reactive agility, passing time, and passing accuracy. Participants of this study were Malaysian high school soccer players (N=64), with mean age of 15.31±1.83, who were assigned randomly into four groups: three experimental groups and one control group. …

Monitoring Relationships Between Corticosterone And Snake Fungal Disease In Timber Rattlesnakes (Crotalus Horridus) In Western Kentucky, John Bromley Hewlett

Murray State Theses and Dissertations

Over the past four decades, emergent fungal diseases have been the most devastating relative to species declines and extinctions. While most research has focused on fungal diseases affecting amphibians and bats, less has focused on diseases like snake fungal disease (SFD), caused by Ophidiomyces ophiodiicola (Oo). SFD was first described in 2006 in North America within a Timber rattlesnake (Crotalus horridus) population in New Hampshire. Since then SFD has been documented in 19 US states, one US territory (Puerto Rico), and Europe. SFD causes high mortality in some species, including the Eastern Massasauga rattlesnakes (Sistrurus …

Males Of The Wolf Spider Rabidosa Rabida Use Two Mechanisms To Stun Females During Copulation, Daniel Schoenberg

Murray State Theses and Dissertations

The differences in energetic input between the sexes required to produce gametes translates to the differences in reproductive behavior and overall mating systems seen in a species. Females generally produce a few energetically and resource expensive eggs and typically choose a high-quality suitor to ensure she has high quality offspring. In contrast, males produce abundant energetically cheap sperm and attempt to fertilize as many eggs as possible in as many females as possible. Both sexes are trying to maximize their inclusive fitness, but the dichotomy of interests can lead to sexual conflict and perhaps extreme or unusual behaviors such as …

Morphological Variance In Mouthparts And Foraging Behavior In Bumblebees, Ye Jin Lee

Honors Theses

Bumblebees (Hymenoptera: Apidae: Bombus) show an incredible degree of size variation within and between species. Individuals from the same hive may vary up to 10-fold in mass. This variation allows individuals to specialize in foraging on different flowers suited to their morphology. However, as different species have different foraging behaviors, their variation in mouthparts and scaling of mouthparts to body size may have been under different kinds of stabilizing selection as they adapted to collect nectar from flowering plants over evolutionary time. Here, we examined the scaling relationships between body size and mouthpart structures, and the variation in mouthpart shape …

Effects Of Integrin Subtypes On Migration And Invasion In Primary Lung Cancer Cells In Advanced Stage And Inhibitory Effect Of Millettocalyxin B, Pennapa Lafauy

Chulalongkorn University Theses and Dissertations (Chula ETD)

Integrin-targeting compounds have shown clinically significant benefits in many patients. Here, we examined the activity of millettocalyxin B, extracted from the stem bark of Millettia erythrocalyx, in lung cancer cells. The viability of human lung cancer cell was investigated by the 3-(4,5- dimethylthiazol-2-yl)-2,5diphenyl tetrazoliumbromide (MTT) assay. Migration and invasion assays were performed. Phalloidin-rhodamine staining was used to determine the formation of filopodia. Western blot analysis was used to identify the signaling proteins involved in migration regulation. Non-toxic concentrations (0-25 μM) of millettocalyxin B reduced migration and invasion of lung cancer A549 cells. Filopodia were significantly reduced in millettocalyxin B treated …

Role Of P53 Deficient Clonogenic Endothelial Cells In The Pathogenesis Of Pulmonary Hypertension, Aneel Roy Bhagwani

Theses and Dissertations

Pulmonary Artery Hypertension (PAH) is a progressive disorder of the small vessels of the pulmonary vasculature. It is characterized by occlusive vascular remodeling leading to right heart failure and ultimately death. PAH pathogenesis involves a multifactorial etiology leading to formation of occlusive lesions in small (preacinary) pulmonary arteries. These lesions contain hyper-proliferative, apoptosis resistant endothelial cells (ECs), smooth muscle cells and fibroblasts. ECs play a major role in the development of PAH. These ECs in vascular lesions are primitive and express progenitor cell markers, including CD117 (c-kit).

We have isolated clonally expanded, c-kit+ ECs from the lungs of Green Fluorescent …

A Novel Review Of Heat Shock Protein 110 Kda: A Basis For Research And Continued Experimentation Through Biochemical Analysis, Crist W. Cuffee

Theses and Dissertations

Heat shock protein 110 kDa, Hsp110, is a distinct cellular protector, different in form and function from Hsp70, a close relative of Hsp110. Functioning primarily as a holdase or in tandem with other molecular chaperones, a review of current accomplishments elucidates the uniqueness of this protein and the continued mysteries that surrounds it. Found only in eukaryotes, Hsp110 has been linked to many diseases, ranging from parasitic infection to neurodegenerative disorders. While still lacking, studies of this protein have provided much in the realm of speculation on the mechanisms behind Hsp110s’ contribution to different pathologies. This review will serve as …

Muscarinic Excitation Of Dopamine Neurons In The Ventral Tegmental Area Via Activation Of A Trpc-Like Cation Conductance, Yu Tzu Chen

Theses and Dissertations

Dopaminergic (DA) neurons in the ventral tegmental area (VTA) play a crucial role in reward and motivational behaviors, including the development of drug addictions. VTA DA neurons receive excitatory cholinergic inputs from the mesopontine tegmentum. Blockage of the M5 muscarinic receptor in DA neurons has been shown to attenuate drug-induced DA release and abuse-related behaviors, but the molecular mechanism is unknown. In this study, experiments were designed to identify the electrophysiological effects of muscarinic agonism in the modulation of action potential kinetics and firing patterns in VTA DA neurons of mice. Pharmacology of the muscarinic receptor-evoked current was also characterized. …

Clinical Significance, Functional Role And Molecular Mechanism Of 2’-O-Methyltransferase Ftsj3 In Promoting Cancer Progression, Morenci Manning-Powell

Wayne State University Dissertations

2’-O-methylation (2’-O-Me), one of the most common modifications within RNA, has multiple roles in modulating RNA structure, stability, and interactions, as well as gene transcription and translation. We previously performed integrative genomic and transcriptomic analysis of 58 RNA methyltransferases, and identified FTSJ3 (FtsJ RNA 2ʹ-O-methyltransferase 3) as significantly amplified/overexpressed in breast cancer. Knockdown of FTSJ3 inhibits breast cancer cell growth in vitro. However, the clinical significance, functional role, and molecular mechanism of FTSJ3 in human cancer remains to be elucidated. In the present study, we first analyzed the differential mRNA and protein expression of FTSJ3 between tumor and normal tissues …

Morphological Changes In Dorsal Root Ganglia Macrophages Associated With Neuropathic Pain Mechanisms Suggest A Novel Target For Chronic Pain Therapy, Emily Kussick

CMC Senior Theses

The present study examined morphological changes in the dorsal root ganglia (DRG) following an innate immune stimulus. The importance of the DRG has increasingly become recognized in pain processing as more than just the home of primary afferent cell bodies. All sensory information passes through the DRG via the primary afferents, and on to the spinal cord. The primary afferents synapse with second-order neurons in the spinal cord that ascend towards the brain, where they transmit the pain signal to the limbic forebrain and/or the somatosensory cortex for processing. The DRG is an interesting niche to study at as it …

Characterization And Reliability Of The Work-Time Relationship During Arm-Cranking, Tristan Starling-Smith

Electronic Theses and Dissertations, 2020-2023

INTRODUCTION: The critical power (CP) exercise test provides 2 measures, CP and anaerobic working capacity (AWC). CP represents a fatigue threshold that separates the heavy and severe exercise domains, while AWC represents the finite amount of work that can be done above CP. A relatively new protocol for estimating CP has emerged and is described as the 3-minute all-out test (3MT). While this lower body 3MT test has been examined for validity and reliability, very little has been done for the upper body. PURPOSE: Examine the reliability of EP and WEP from the 3MT during upper-body ergometry. This study also …

Influence Of Body Composition, Velocity Profiles, And Sex-Related Differences On Army Combat Fitness Test Performance, David Boffey

Electronic Theses and Dissertations, 2020-2023

The Army Combat Fitness Test (ACFT) will become the United States Army's mandatory physical fitness test in March of 2022. The purpose of this study was to determine the relationship between ACFT performance and both body composition and velocity profiles, and to determine sex differences for these variables. Data was collected in November 2020 (Fall) and March 2021 (Spring) from male (n = 55) and female (n = 17) Army Reserve Officers' Training Corps (ROTC) cadets. Body composition was assessed with a bioelectrical impedance spectroscopy (BIS) device, and cadets completed a squat jump (SJ) force-velocity profile (FVP) and a hex …

Comparison Of The Times To Exhaustion, Physiological, Perceptual, And Neuromusuclar Responses During Constant Heart Rate, Constant Vo2, And Constant Power Exercise, Pasquale Jiovanni Succi

Theses and Dissertations--Kinesiology and Health Promotion

The purposes of this study were to: 1) Determine if there are differences in the sustainability of exercise when anchored at critical heart rate (CHR), the VO₂ associated with CHR (VO₂CHR), or the power output associated with CHR (PCHR); 2) examine the patterns of responses in power output, metabolic (heart rate [HR], VO₂, respiration rate [RR]), neuromuscular (electromyographic [EMG] amplitude [AMP], mean power frequency [MPF], mechanomyographic [MMG] AMP and MPF, perceptual (rating of perceived exertion [RPE]), and muscle oxygenation responses (%SmO₂) during exercise anchored by HR, VO₂, and power output; and …

Examination Of Sex- And Limb-Specific Fatigue During Unilateral, Isometric Forearm Exercise, Caleb Christian Voskuil

Theses and Dissertations--Kinesiology and Health Promotion

The purpose of this study was to examine the effects of unilateral, isometric handgrip holds to failure for the dominant (Dm) and non-dominant (NDm) limb on ipsilateral ([IPS] exercised side) and contralateral ([CON] non-exercised side) performance fatigability. Twenty individuals participated in this study (Men [n =10]; Women [n = 10; Composite Demographics: Age: 22.2 years; Height: 174.4 cm; Body Mass: 75.0 kg) and completed three visits. Two, 6 s maximal voluntary isometric contractions (MVICs) for the Dm and NDm limb were performed during visit 1, followed by a familiarization of the fatigue test. Visits 2 and 3 included an isometric, …

Quadriceps Recruitment And Twitch Properties In Lean And Obese Women Before And After Fatiguing Exercise, Rebekah Seay Carpenter

Theses and Dissertations--Kinesiology and Health Promotion

Skeletal muscle strength, specifically that of the anti-gravitational muscles such as the quadriceps, is essential for performing dynamic tasks including walking, navigating stairs, and transitioning between sitting and standing. Obesity has been shown to negatively impact musculoskeletal function and decrease quadriceps strength relative to body weight. Additionally, obesity has been linked with higher rates of fatigue and lower strength outcomes following a test of muscular endurance of the quadriceps. Since obesity lowers relative strength, it is likely that quadriceps muscle weakness contributes to reduced physical function of dynamic tasks. However, to date, the precise mechanism for obesity-induced muscle weakness has …

Novel Mammalian Models For Understanding And Treating Spinal Cord Injury, Michael B. Orr

Theses and Dissertations--Physiology

Spinal cord injury (SCI) is devastating and often leaves the injured individual with persistent dysfunction. The injury persists because humans have poor wound repair and there are no pharmacologic treatments to induce wound repair after SCI. The continued efforts to discover therapeutic targets and develop treatments heavily relies on animal models. The purpose of this project is to develop and study novel mammalian models of SCI to provide insights for the development and effective implementation of SCI therapies.

Lab mice (Mus musculus) are a powerful tool for recapitulating the progression and persistent damage evident in human SCI, but …

The Effect Of High-Fat Style Diet And Ovariectomy On Inflammation And Baroreflex Control Of Renal Sympathetic Nerve Activity, Yamuna Sucedaram

Student Works (2020-2029)

High fat diet-induced visceral obesity, renal inflammation and injury can lead to augmented renal sympathetic nerve activity (RSNA). The mechanism for the increase in RSNA appears to be due to the blunting of the arterial and cardiopulmonary baroreflexes resulting from an inflammatory response. This study aimed to investigate the effect of high fat style diet (HFSD) and loss of ovarian function on structural changes and inflammatory response in the kidney as well as in the liver; and on the low- and high�pressure baroreflex control of RSNA. To that end, ovary-intact and ovariectomised (OVX) Sprague Dawley rats received a normal diet …

Effects Of Voluntary Exercise On Pressure-Induced Natriuresis In Hypertensive Female And Male Rats, Keshari Sudasinghe

All Graduate Theses, Dissertations, and Other Capstone Projects

Hypertension (HT) is one of the major diseases associated with the circulatory system and remains a public health problem with a high rate of prevalence in the adult population in the USA. The pressure natriuresis mechanism which is Na+ excretion in respond to rise in blood pressure acts as a long-term regulator of arterial blood pressure. Recent studies have demonstrated that impairment of this long-term pressure natriuresis mechanism is involved in hypertension (HT) and many other renal disorders. Exercise is a nonpharmacological treatment to help control HT, and exercise has a beneficial effect on vascular health, endothelial functions, arterial stiffness, …

Search For New Fertility Markers In Bull Sperm, Saulo Menegatti Zoca

Electronic Theses and Dissertations

Sperm are stored for extended periods of time in the epididymis, but upon ejaculation motility is increased and lifespan is decreased. After insemination, sperm must traverse the female barriers and undergo capacitation to complete fertilization; however, there are differences in fertility even among bulls that successfully pass a breeding soundness exam. For any potential marker of fertility, there must be variability expressed among animals. The series of studies in this dissertation had the objective 1) to evaluate differences between epididymal and ejaculated sperm and respective fluids proteins to understand increased longevity of epididymal sperm; 2) to evaluate the potential of …

Short-Term Fasting And Autonomic Control, Joshua Gonzalez

Dissertations, Master's Theses and Master's Reports

Obesity is a chronic metabolic disorder associated with increased risk of cardiovascular disease. Evidence suggests that chronic intermittent fasting improves cardiometabolic health and reduces arterial blood pressure. However, the mechanisms underlying the reductions in blood pressure and improved cardiovascular health observed from chronic fasting studies remain unclear. The autonomic nervous system has a central role in the regulation of blood pressure and is essential for cardiovascular homeostasis. We conducted a study to investigate how acute fasting influences autonomic control of blood pressure at rest and during stress. Twenty-five young, healthy, normal weight, normotensive participants were tested twice, once in the …