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Safety Of Blood Flow Restriction Training For Musculoskeletal Disorders: An Evidence-To-Practice Review, Breanna Ferguson, Erica Schulman, Matthew Zimmerman, Zachary K. Winkelmann

Clinical Practice in Athletic Training

Blood flow restriction training (BFRT) is low-level resistance training while partially occluding proximal blood flow. It is well documented that this style of training leads to increased muscle size as well as strength. It is theorized that these size and strength gains are due in part to the decreased oxygen environment. This results in increased muscular stress without the need for increased external load making this style of resistance training ideal for individuals who have restrictions due to musculoskeletal disorders. The guiding systematic review examined the safety of BFRT when used as a therapeutic intervention for patients with a variety …

Exertional Heat Stroke In A Male High School Runner With Disordered Eating: A Disablement Model Case Study, Brittni G. Hoover, Rebecca M. Hirschhorn, Allison B. Smith, Amy F. Hand

Clinical Practice in Athletic Training

A 16-year-old male high school cross country athlete collapsed at the end of an afternoon 5K cross country meet. The patient was unable to stand, and after outwardly displaying confusion and agitation, his coaches sought assistance from the athletic trainers (ATs). The patient consumed one cup of water and a granola bar on the day of the meet. The ambient air temperature was 90°F with 69% humidity. Differential diagnoses included exertional heat stroke (EHS), exertional heat exhaustion, exertional collapse associated with sickle cell trait, heat syncope, dehydration, malnutrition, and hypoglycemia. Approximately 20 minutes passed between activation of the emergency action …

Characteristics Of Sickle Cell Trait Policies And Procedures At Ncaa Division I Institutions, H Parker, B J. Warner, D M. Gallegos, S A. Cage

Clinical Practice in Athletic Training

Context: In 2010, the National Collegiate Athletic Association (NCAA) introduced a proposal addressing sickle cell trait screening. This proposal later became a policy that required student-athletes in their first year of eligibility to provide their institution with accurate information regarding their sickle cell trait status. There does not appear to be a recent study published that has examined the sickle cell trait policies and procedures currently being used by NCAA Division I institutions. The purpose of this study was to describe the characteristics of sickle cell trait policies and procedures at NCAA Division I institutions. Methods: We used a web-based …

Microvascular Consequences Of Engineered Nanomaterial Inhalation Exposure And Subsequent Reproductive Outcomes, Krista Lee Garner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nano-titanium dioxide (nano-TiO₂) is a commonly utilized engineered nanomaterial found ubiquitously in consumer products. While benefits of ENM utilization in are undeniable, nanoparticle inhalation has been linked to cardiovascular consequences and altered microvascular responses. Given these outcomes, it is important to explore vulnerable timepoints, such as gestation, as robust vascular adaptations are essential for maternofetal health.

The first aim of this dissertation was to assess myogenic responsiveness following maternal nano-TiO₂ inhalation. Fetal aortic responses were evaluated on gestational day (GD) 20 to assess how exposure may impact the F1 generation. Myogenic responsiveness within the uterine radial arteries …

Sleep And Cardiovascular Reactivity To An Acute Virtual Stressor, Alaina Grace Tiani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Inappropriate response and magnitude of cardiovascular reactivity (CVR) to stress is a proposed mechanism through which environmental stressors are linked with poor cardiovascular health outcomes (Chida & Steptoe, 2010; Krantz & Manuck, 1984). Studies of reactivity to various laboratory tasks commonly control for factors known to influence CVR such as smoking, medication use, caffeine intake, and BMI; however, few have considered the influence of sleep on CVR. In order to determine whether sleep characteristics need to be assessed and considered in studies of CVR, this study aimed to examine the association between indices of sleep quality and the magnitude and …

Mechanistic Insight Into Chronic Stress-Induced Cerebrovascular Dysfunction, Emily Nicole Burrage

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite decades of research psychological stress remains a prevalent problem in society, often diminishing quality of life and increasing the risk of developing dementia. The brain plays a central role in the response to stress and as it is unable to store energy substrates, the brain relies heavily on dynamically regulated blood flow. Therefore, it is important that there is a tight coupling between neural activity and cerebral blood flow to meet metabolic demands and maintain critical brain function. It has been established that chronic psychological stress has detrimental effects on cerebrovascular function, potentially through increased superoxide anion production through …

Assessing The Behavioral Response Of Westslope Cutthroat Trout To Olfactory Cues In A Two-Choice Y-Maze, Hannah Marie Condron

EWU Masters Thesis Collection

The goal of this study was to determine which olfactory cues were most attractive to Westslope Cutthroat Trout Oncorhynchus clarkii lewisi (WCT) in a two-choice Y-maze. WCT used in this study were from Kings Lake and Paqua Creek in Pend Oreille County, Washington, and tested in 2018 and 2019. The different odorants tested in this study were population-specific pheromones produced by WCT from the same population, conspecific pheromones produced by WCT from the second population, and water obtained from each population’s natal stream. The water Kings Lake WCT were reared in for one year at the Spokane Hatchery was also …

Cryo-Em Structure Of Transmembrane Aaa+ Protease Ftsh In The Adp State, Wu Liu, Martien Schoonen, Tong Wang, Sean Mcsweeney, Qun Liu

Advanced Science Research Center

AAA+ proteases regulate numerous physiological and cellular processes through tightly regulated proteolytic cleavage of protein substrates driven by ATP hydrolysis. FtsH is the only known family of membrane-anchored AAA+ proteases essential for membrane protein quality control. Although a spiral staircase rotation mechanism for substrate translocation across the FtsH pore has been proposed, the detailed conformational changes among various states have not been clear due to absence of FtsH structures in these states. We report here the cryo-EM structure for Thermotoga maritima FtsH (TmFtsH) in a fully ADP-bound symmetric state. Comparisons of the ADP-state structure with its apo-state and a substrate-engaged …

Inhibition Of Bruton Tyrosine Kinase Reduces Neuroimmune Cascade And Promotes Recovery After Spinal Cord Injury, Chen Guang Yu, Vimala Bondada, Hina Iqbal, Kate L. Moore, John C. Gensel, Subbarao Bondada, James W. Geddes

Physiology Faculty Publications

Microglia/astrocyte and B cell neuroimmune responses are major contributors to the neurological deficits after traumatic spinal cord injury (SCI). Bruton tyrosine kinase (BTK) activation mechanistically links these neuroimmune mechanisms. Our objective is to use Ibrutinib, an FDA-approved BTK inhibitor, to inhibit the neuroimmune cascade thereby improving locomotor recovery after SCI. Rat models of contusive SCI, Western blot, immunofluorescence staining imaging, flow cytometry analysis, histological staining, and behavioral assessment were used to evaluate BTK activity, neuroimmune cascades, and functional outcomes. Both BTK expression and phosphorylation were increased at the lesion site at 2, 7, 14, and 28 days after SCI. Ibrutinib …

Caffeinated Gum Does Not Influence Rpe-Regulated Cadence In Recreationally-Active, College Females Regardless Of Habitual Caffeine Consumption, Maria L. Mask, Bandar A. Alghamdi, Lauren G. Killen, James M. Green, Eric K. O'Neal, Hunter S. Waldman

International Journal of Exercise Science

International Journal of Exercise Science 14(2): 1375-1387, 2021. Caffeine (CAF) has been extensively studied for its ergogenic and analgesic effects during exercise. However, the majority of these studies have been conducted in male populations. This study investigated the effects of acute CAF chewing gum on self-selected exercise intensity during a rating of perceived exertion (RPE) production trial in active females (n = 16, 21.0 ± 2.8 y). Data were also analyzed based on habitual CAF consumption level. Participants completed a V̇O_2peak trial, followed by a familiarization and two randomized, triple-blinded experimental RPE production trials on an arm ergometer …

Cardiorespiratory Function In Young Adults With A History Of Covid-19 Infection, Danielle Arcidiacono

Theses - ALL

Objective. Respiratory complications may persist several months into the recovery period following COVID-19 infection. This study evaluated respiratory function and oxygen saturation variability between young adults with a history of COVID-19 infection and controls. Associations between cardiorespiratory function with potential biobehavioral correlates of COVID-19 infection were also explored.Methods. 57 adults ages 18 to 65 participated in this study (24 COVID+, 33 Control). Spirometry was used to assess pulmonary function volumes of forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), FEV1/FVC and peak expiratory flow (PEF). Exhaled nitric oxide (FeNO) was measured using the NiOX VERO, a handheld …

Late-Life Exercise Mitigates Skeletal Muscle Epigenetic Aging, Kevin A. Murach, Andrea L. Dimet-Wiley, Yuan Wen, Camille R. Brightwell, Christine M. Latham, Cory M. Dungan, Christopher S. Fry, Stanley J. Watowich

Center for Muscle Biology Faculty Publications

There are functional benefits to exercise in muscle, even when performed late in life, but the contributions of epigenetic factors to late-life exercise adaptation are poorly defined. Using reduced representation bisulfite sequencing (RRBS), ribosomal DNA (rDNA) and mitochondrial-specific examination of methylation, targeted high-resolution methylation analysis, and DNAge™ epigenetic aging clock analysis with a translatable model of voluntary murine endurance/resistance exercise training (progressive weighted wheel running, PoWeR), we provide evidence that exercise may mitigate epigenetic aging in skeletal muscle. Late-life PoWeR from 22–24 months of age modestly but significantly attenuates an age-associated shift toward promoter hypermethylation. The epigenetic age of muscle …

Effects Of Respite Music On Repeated Upper-Body Resistance Exercise Performance, Jonathan T. Lehman, Brooklyn G. Whitmire, Rebecca R. Rogers, Tyler D. Williams, Christopher G. Ballmann Facsm

International Journal of Exercise Science

International Journal of Exercise Science 15(7): 79-87, 2022. The purpose of this study was to investigate the effects of self-selected respite music on upper-body resistance exercise performance. In a crossover, counterbalanced study design, resistance-trained males (n = 10) participated in two bench press trials each with a different condition: 1) No music (NM), 2) Listening to respite music (RM; i.e. during rest periods). Following a warm-up, participants completed 3 sets × repetitions to failure (RTF) at 75% of 1-RM separated by 2 minutes of rest. During the 2-minute rest, participants either listened to NM or RM until the next …

An Exploratory Comparison Of Subjective Mental Fatigue Following A Task Designed To Replicate The Observation Of Game Film, Anthony Magdaleno, Lenny Wiersma, Barbara B. Meyer

International Journal of Exercise Science

International Journal of Exercise Science 15(6): 25-35, 2022. Laboratory-induced subjective mental fatigue (MF) has been shown to decrease sport-related performance (23, 38), yet there is a lack of research identifying tasks in real-world sport environments that induce MF (37). Since the identification of real-world tasks that induce MF may inform activities undertaken in the daily training and competition environments, the purpose of the current study was to compare changes in MF following a task designed to replicate the observation of game film to changes in MF following completion of a laboratory-based task (e.g., Stroop test). On separate counterbalanced visits, participants …

The Influence Of Relaxing And Self-Selected Stimulating Music On Vertical Jump Performance In Male Volleyball Players, Simon Gavanda, T. J. Hosang, S. Wagener, N Sönmez, I. Kayser, A. J. Knicker

International Journal of Exercise Science

International Journal of Exercise Science 15(6): 15-24, 2022. Most research concerning the effects of music on physical performance was conducted using endurance parameters. This study investigated the effects of relaxing (RLX) vs. self-selected stimulating music (SM) vs. no music (NM) on jump height (JH), jump power (PWR), and average rest period between jumps (RP) in 13 athletes (age: 25.5 ± 2.6 years). After a warm-up and listening to music (1 min) or NM, participants completed five squat jumps on a force plate. Psychological ratings of mood were assessed using a questionnaire before warm-up and after jumping. A one-way ANOVA was …

Functional Movement Screen In High School Basketball Players, Nikole J. Keil, Lynn A. Darby, K. Todd Keylock, Jessica Kiss

International Journal of Exercise Science

International Journal of Exercise Science 15(6): 1-14, 2022. Pre-participation screening and tracking of an athlete’s functional status during a competitive season is essential to maintaining optimal performance. The sport of basketball had the third highest number of boys and girls participating during the 2018-2019 season (23), which typically occurs October to March each year. The Functional Movement Screen™ (FMS; 10) has been administered to some youth athletes from various sports, however, both males and females from basketball have not been studied extensively. The purposes of this study were: 1) to assess functional movements before and after the natural progression of …

The Health And Fitness Characteristics Of Civilian Jailer Recruits Prior To Academy Training, Robert G. Lockie, Matthew R. Moreno, Joseph M. Dulla, Robin (Rob) Marc Orr, J. Jay Dawes, Karly A. Rodas

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 58-78, 2022. Civilian jailer (CJ) recruits may not complete fitness testing within their hiring process. As certain job tasks in custody/jail facilities can be physically demanding (e.g., inmate restraint), better health and fitness should help a recruit graduate from a CJ academy and be prepared for the occupation. This study investigated the health and fitness characteristics of CJ recruits entering academy training, detailed between-sex differences, and categorized recruits relative to population norms. Retrospective analysis was conducted on data from 89 CJ recruits (48 males, 41 females) across three academy classes from one law enforcement …

Relationships Between Hex Bar Deadlift One-Repetition Maximum And Maximal Isometric Pulls, Brandon A. Miller, Eliott Arroyo, Emily C. Tagesen, Adam R. Jajtner

International Journal of Exercise Science

International Journal of Exercise Science 15(4): 45-57, 2022. This study sought to determine relationships between hexagonal barbell (HBB) deadlift one-repetition maximum (1-RM) and force-time characteristics of maximal isometric pulls. Twenty-three healthy adults (13 men [8 trained], 10 women [4 trained]) completed three visits consisting of a familiarization and anthropometrics session, a HBB deadlift 1-RM session, and a performance session with three maximal isometric pulls at three positions: lift-off (FLOOR), knee-passing (KNEE), and mid-thigh (MT). Correlation analyses assessed relationships between 1-RM and force-time characteristics at each position with significance set a priori at α ≤ 0.05. Correlation coefficients between 1-RM and …

Sleep And Risk For Metabolic Syndrome, Hypertension, Diabetes And Obesity Among Community-Dwelling Older Adults, Philip Zendels, Trudy L. Moore-Harrison, Jane F. Gaultney

International Journal of Exercise Science

International Journal of Exercise Science 15(3): 88-102, 2022. Older adults often face a variety of health problems that are found less frequently in younger populations. Metabolic syndrome and other related diseases are common due to a variety of age and lifestyle factors. Sleep, often operationalized only as duration, quality, or apnea diagnosis, is associated with worse health outcomes across the lifespan. However, sleep is multi-faceted and may require a collection of measures in order to reflect this. This study examined a suite of self-reported sleep habits (risk for sleep apnea, night time duration, nap duration, quality, timing, and consistency of …

Cardiovascular, Metabolic And Perceptual Responses To Preferred Walking Speed At Different Inclines And Post Exercise Postural Control In Healthy College Age Adults, Meir Magal, S. Leanna Fann, Kathleen S. Thomas

International Journal of Exercise Science

Although several studies investigated heart rate (HR) and metabolic responses to preferred walking speed (PWS), there is a limited amount of data on PWS responses during varying inclines. Further, there is no data pertaining to the impact of PWS at various inclines on postural control. The purpose of the study was to measure cardiovascular, metabolic, perceptual, and postural impacts of walking at PWS at various inclines. Twenty-one participants completed two lab sessions, seven days apart. On day one, PWS on the treadmill and maximal oxygen consumption (O_2max) were established for each participant. On day two, using a counter …

Effects Of Low Dose Caffeine On Post-Exercise Heart Rate Variability: A Double-Blind Placebo-Controlled Trial, Cagney T. Sargent, Tareq K. Shahbal, Andres E. Carrillo, Tania Amorim, Jason R. Edsall, Emily J. Ryan, Edward J. Ryan

International Journal of Exercise Science

International Journal of Exercise Science 15(2): 103-112, 2022. Caffeine may impact post-exercise heart rate variability (HRV); although, studies have yielded inconsistent findings. We examined the effects of low dose caffeine on post-exercise HRV. Healthy, college-aged adults [n = 18; age: 22.1 ± 2.6 years; BMI: 26.9 ± 4.3 kg/m²; estimated maximal oxygen consumption (VO₂max): 45.1 ± 8.3 ml∙kg^-1∙min^-1] participated in a repeated-measures, double-blind, placebo-controlled trial. During the experimental trials, participants were fitted with a heart rate monitor and a mouthpiece with a one-way nonrebreathing valve and then rested for 10 min …

Forces At The Feet, Gait Timing, And Trunk Flexion/Extension Excursion While Walking With A Gear Belt Or Gear Vest Load, Shane S. Maher, Laura L. Dill, Jennifer L. Hein, Jeff A. Nessler, Deanna J. Schmidt

International Journal of Exercise Science

International Journal of Exercise Science 15(1): 36-44, 2022. Law enforcement personnel often carry gear loads, which have a history of causing low back pain. The aim of this study was to evaluate the differences in gait and trunk posture for gear load carried on a gear belt and a gear vest. Twenty-nine participants performed load carriage in three conditions: a no load control trial (C), a symmetrically loaded gear belt (GB), and an anterior-loaded gear vest (ALV). Gear conditions had 9.07 kg of additional mass. Motion capture and insole force sensors were used to collect data while participants walked on …

Identifying And Knocking Out Non-Visual Opsins In The Optic Tectum Of Zebrafish Larvae, Sarah Jarrett

Undergraduate Honors Theses

Deep brain photoreceptors (DBPs) are non-visual photoreceptors found in the brains of non-mammalian vertebrates like zebrafish. While using optogenetic techniques in our lab to photo-activate the ear (by expressing the light-gated channel: channelrhodopsin) in efforts of identifying optic tectum (OT) neurons that respond to and integrate auditory and visual stimuli, we found that violet light was able to elicit OT neuronal activity. OT activity was also detected in cases when visual and auditory stimulation was not possible due to removal of the eyes and absence of the exogenously expressed channelrhodopsin, respectively. Analyzing OT single-cell sequencing data of 7day post fertilization …

Student Pharmacists’ Emotional Responses And Coping During The Covid-19 Pandemic, Hannah E. Johnson, Deaundre Bumpass, Aric Schadler, Jeffrey Cain

Journal of Wellness

Introduction: Health professions students, including student pharmacists, have been impacted by the coronavirus disease 19 (COVID-19 pandemic) as schools have transitioned to remote learning and cancelled milestone events. During times of crises, media consumption and hobby participation also impact well-being. The adverse emotional responses and coping strategies of student pharmacists amidst the COVID-19 pandemic have not been evaluated, nor have factors that may contribute to emotional responses. The purpose of this study is to determine Doctor of Pharmacy students’ emotional responses and coping precipitated by the COVID-19 pandemic, and the influence of media use, working status, and participation in hobbies. …

Genetic Effects Mediated Through Epistatic Networks Onto Metabolic Traits, Juan Francisco Macias-Velasco

Arts & Sciences Electronic Theses and Dissertations

Predicting variation in complex traits from DNA sequence is a major public health goal, but our understanding of the genotype-to-phenotype relationship is incomplete. It will remain so unless we can adequately integrate genetic, epigenetic, and environmental information into a systems level framework. In a step towards that goal, quantitative trait mapping studies have attempted to account for environmental factors such as sex and diet, and epigenetic factors such as allelic parent-of-origin effects. Several studies used an advanced intercross of the LG/J and SM/J inbred mouse strains to unravel the genetic architecture of multiple metabolic traits. These studies found that parent-of-origin …

Relationship Of Winter Hair Coat Shedding Ability Of Purebred Angus Dams With Mineral Status, Apparent Forage Digestibility, Uterine Artery Hemodynamics, And Calf Growth And Development, Randy Hunter Burnett

Theses and Dissertations

Winter hair coat shedding ability in southern U.S. cattle production has previously been associated with differences in adjusted birth and weaning weights of calves from dams that shed by June or later (Gray et al., 2011; Cauble et al., 2019). This study was developed to research possible avenues of why these relationships exist with winter hair coat shedding ability. The primary objectives of the following experiments were to: 1) evaluate how mineral status relates to winter hair coat shedding ability, 2) evaluate how winter hair coat shedding ability relates to apparent digestibility of forage, 3) evaluate winter hair coat shedding …

The Art Of Amphibian Conservation: Linking In-Situ And Ex-Situ Populations Of Endangered Species Through Genome Banking, Isabella Joann Burger

Theses and Dissertations

Limited breeding success in captive breeding programs has necessitated the development of assisted reproductive technologies (ART) to preserve and increase genetic variation and population numbers of both captive and wild amphibian groups. ART has been shown to be successful in numerous anuran species, and current studies focus on the application of ART in ex-situ populations. The focus of this project is to show that linking in-situ and ex-situ amphibian populations through sperm cryopreservation, genome banking, and in-vitro fertilization is possible, with the goal of increasing gene diversity throughout groups in order to produce self-sustaining, wild populations in the future. Specific …

Evidence Of Myomir Regulation Of The Pentose Phosphate Pathway During Mechanical Load-Induced Hypertrophy, Taylor Valentino, Vandre C. Figueiredo, C. Brooks Mobley, John J. Mccarthy, Ivan J. Vechetti Jr.

Physiology Faculty Publications

Many of the molecular and cellular mechanisms discovered to regulate skeletal muscle hypertrophy were first identified using the rodent synergist ablation model. This model reveals the intrinsic capability and necessary pathways of skeletal muscle growth in response to mechanical overload (MOV). Reminiscent of the rapid cellular growth observed with cancer, we hypothesized that in response to MOV, skeletal muscle would undergo metabolic programming to sustain increased demands to support hypertrophy. To test this hypothesis, we analyzed the gene expression of specific metabolic pathways taken from transcriptomic microarray data of a MOV time course. We found an upregulation of genes involved …

Alterations In Glycemic Variability, Vascular Health, And Oxidative Stress Following A 12-Week Aerobic Exercise Intervention-A Pilot Study, Joshua R. Sparks, Mark A. Sarzynski, J. Mark Davis, Peter W. Grandjean, Xuewen Wang

International Journal of Exercise Science

International Journal of Exercise Science 14(3): 1334-1353, 2021. The state of being overweight or obese leads to an increased risk of development of cardiometabolic disease. Increases in glycemic variability have been associated with greater induction of oxidative stress and declined vascular health, which may be exacerbated by higher weight status and improved through exercise. The purpose of this study was to examine the impact of a twelve-week aerobic exercise intervention on continuous glucose monitor (CGM) assessed glucose concentrations and glycemic variability, and biomarkers of vascular health and oxidative stress in overweight or obese adults. Eight adults (Age = 48.9 ± …

Acute Changes In Arterial Function Post High-Intensity Lower Extremity Cycling, Alyssa Weatherholt, William R. Vanwye, Jeffery L. Short, Alyssa A. Andreis, Guoyuan Huang

International Journal of Exercise Science

International Journal of Exercise Science 14(2): 1363-1374, 2021. The purpose of this study was to assess the acute arterial blood flow velocity of the lower extremity (LE) immediately after a bout of high-intensity LE cycling exercise. Twenty-eight healthy males (n = 14) and females (n = 14) aged 20.9 ± 1.7 years participated in this study. All subjects performed a single bout of high-intensity cycling (70% of HRR) for 45 min. The subjects' LE blood flow velocity, heart rate (HR), systolic blood pressure (SBP), and oxygen saturation (SpO₂) were measured at rest, immediately post-, post-15 min., …