Digital Commons Network^™

Mitochondrial Genome Polymorphism In Lolium Perenne, Y Shimamoto, Y Tominaga, M Sato, T Mikami

IGC Proceedings (1977-2023)

The restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) of perennial ryegrass (Lolium perenne L.) were investigated to elucidate the genetic relatedness among the 128 cultivars including diploid and tetraploid. Many patterns of RFLPs were observed and allowed assigning of the cultivars into the main eight haplotypes of mitochondrial genome relatedness. The American cultivars were classified into haplotype I and VIII which were remote at the mitochondrial genome from each other, the European ones were distributed to all haplotypes and the tetraploid ones were mostly assigned into the haplotype V. The assessment of mtDNA RFLPs may be a …

Inbreeding Of Some Populations In The Genus Agrostis L., Zorica Tomic, Gordana Surlan Momirovic, Dusica Delic, S Ostojic

IGC Proceedings (1977-2023)

This work presents some properties on produced inbred lines of the 5 best populations of 4 species of Agrostis for breeding purposes. The cultivars of forage grasses should meet a given aim for selection, which is the increasing dry matter and quality. The populations used in this research were the best ones for morphological and productive properties from a previous study. By the process of selfing there have been produced S1 offsprings which appeared to be quite superior in relation to open pollinated offspring from the mother plants. The obtained results were processed by multiple range regression analyses. According to …

The Effect Of Abscisic Acid On The Freezing Tolerance In Lolium Temulentum L., K Tase, H Fujii

IGC Proceedings (1977-2023)

This study was conducted to clarify the effect of abscisic acid (ABA) on the development of freezing tolerance in Lolium temulentum L. We compared the changes of freezing tolerance and endogenous ABA content by the ABA treatment with those by the hardening treatment. Exogenous ABA applications (7.5 x 10-5 M) increased freezing tolerance, but did not completely substitute for the hardening process. This treatment resulted in great increase in endogenous ABA content, as compared with the control or the hardening treatment. Since the relationship between endogenous ABA content and freezing tolerance was not parallel, it is considered that freezing tolerance …

Deep Learning Model For Personalized Prediction Of Positive Mrsa Culture Using Time-Series Electronic Health Records, Masayuki Nigo, Laila Rasmy, Bingyu Mao, Bijun Sai Kannadath, Ziqian Xie, Degui Zhi

Faculty, Staff and Student Publications

Methicillin-resistant Staphylococcus aureus (MRSA) poses significant morbidity and mortality in hospitals. Rapid, accurate risk stratification of MRSA is crucial for optimizing antibiotic therapy. Our study introduced a deep learning model, PyTorch_EHR, which leverages electronic health record (EHR) time-series data, including wide-variety patient specific data, to predict MRSA culture positivity within two weeks. 8,164 MRSA and 22,393 non-MRSA patient events from Memorial Hermann Hospital System, Houston, Texas are used for model development. PyTorch_EHR outperforms logistic regression (LR) and light gradient boost machine (LGBM) models in accuracy (AUROC

When And Why Ecological Systems Respond To The Rate Rather Than The Magnitude Of Environmental Changes, Karen C. Abbott

Faculty Scholarship

Ecologists and conservation biologists have become quite familiar with the concept of tipping points: abrupt changes in an ecosystem's state that occur after a period of relative stasis. Most of the familiar ecological examples of tipping points occur either because a once-stable state has lost stability, or the system has been subjected to a particularly large perturbation and transitions to an alternative stable state, distinct from the pre-perturbed state. A different class of tipping points, known as rate-induced tipping (or r-tipping) points, are likely present in many ecological communities but remain little known in the field. Rate-induced tipping occurs when …

Introduction Of Vegetative And Reproductive Characters Into Trifolium Repens By Interspecific Hybridisation, A H. Marshall, T P.T Michaelson-Yeates, M Meredith, D H. Hides

IGC Proceedings (1977-2023)

Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of …

Colchicine-Doubling Of Germinating Seedlings Of Interspecific Wildrye Hybrids, T A. Jones, D C. Nielson, H Jaussi

IGC Proceedings (1977-2023)

Colchicine has usually been applied to sterile clones of interspecific grass hybrids to restore fertility. However, when hybrids are partially fertile, colchicine can be applied to germinating seedlings. Four populations of Basin wildrye (Leymus cinereus) X beardless wildrye (L. triticoides) were treated with a 0.2% colchicine solution to double chromosome number from 4x=28 to 8x=56. Doubling percentage was 28, 33, 42, and 44% of all root-tips examined for the four populations. When plants without doubled sectors were discarded, doubling percentage increased to 56, 68, 78, and 78%. Plants with doubled sectors have been placed in crossing blocks …

Development Of An Integrated Workflow For Nucleosome Modeling And Simulations, Ran Sun

Doctoral Dissertations

Nucleosomes are the building blocks of eukaryotic genomes and thus fundamental to to all genetic processes. Any protein or drug that binds DNA must either cooperate or compete with nucleosomes. Given that a nucleosome contains 147 base pairs of DNA, there are approximately 4^147 or 10^88 possible sequences for a single nucleosome. Exhaustive studies are not possible. However, genome wide association studies can identify individual nucleosomes of interest to a specific mechanism, and today's supercomputers enable comparative simulation studies of 10s to 100s of nucleosomes. The goal of this thesis is to develop and present and end-to-end workflow that serves …

3d Pictorial Representations Of O2- And Ho2 Compared To Dft Results, Tzu-Hsiang Huang, Joseph D. Alia

Chemistry Publications

The Linnett Double Quartet (LDQ) (1) and Bohr Orbit Bonding (BOB) (2) methods were used to accurately represent the spin density distribution for superoxide (O2 -) and hydroperoxyl radical (HO2). These structural pictures were superimposed on the spin density plot produced by DFT. The Valency Interaction Formula (VIF) method (3) was also applied to these molecules and it matched the NBOs from NBO analysis of DFT results. The interaction between O2 - and H2O were analyzed and the stronger basicity of the beta-spin electrons of superoxide was identified by looking at the LDQ structure and confirmed with NBO analysis. The …

Plants Reduced Nitrous Oxide Emissions From A Northern Great Plains Saline/Sodic Soil, Sharon A. Clay, Thandiwe Nleya, David E. Clay, Deepak Joshi, Dwarika Bhattarai, Shin-Yi Marzano, Bhanu Prakash Petla

Agronomy, Horticulture and Plant Science Faculty Publications

The slowly establishing salt-tolerant perennial grasses reduced nitrous oxide (N2 O- N) emissions from saline/sodic soil compared to barren areas. Other salt-tolerant species may accelerate vegetative establishment and reduce N 2 O-N emissions. In a greenhouse study, barley (Hordeum vulgare L.), Florida broadleaf mustard (Brassica juncea L.), and Kernza intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] were grown for 63 days to compare shoot biomass and chemical composition, N 2 O-N emissions, and the soil microbiome between saline/sodic and productive (non-salt impacted) soils. Emissions were measured six times daily from 1 to 22 and 42 to 63 …

Publications And Other Works By R. F. Diffendal, Jr., Robert F. Diffendal Jr.

School of Natural Resources: Faculty Publications

Articles
Chapters in Books
Books
Field Guides
Geologic Maps
Other Maps
Cover Photographs
Other Photographs
Newspaper and Other Articles
Test-Hole Logs
Television Programs
Geonotes
Abstracts
Reviews
Manuscripts Accepted (A), Submitted (S), or in Preparation (P)
Geologic Maps in Press
Geologic Maps-Nebraska
Public Documents
Miscellaneous Publications
Editing

Updated March 2024

Electronic Relaxation Mechanism Of 9-Methyl-2,6-Diaminopurine And 2,6-Diaminopurine-2′-Deoxyribose In Solution, Luis A. Ortiz-Rodríguez, Naishka E. Caldero-Rodríguez, Sourav Kanti Seth, Karitza Díaz-González, Carlos E. Crespo-Hernández

Faculty Scholarship

Prolonged ultraviolet exposure results in the formation of cyclobutane pyrimidine dimers (CPDs) in RNA. Consequently, prebiotic photolesion repair mechanisms should have played an important role in the maintenance of the structural integrity of primitive nucleic acids. 2,6-Diaminopurine is a prebiotic nucleobase that repairs CPDs with high efficiency when incorporated into polymers. We investigate the electronic deactivation pathways of 2,6-diaminopurine-2′-deoxyribose and 9-methyl-2,6-diaminopurine in acetonitrile and aqueous solution to shed light on the photophysical and excited state properties of the 2,6-diaminopurine chromophore. Evidence is presented that both are photostable compounds exhibiting similar deactivation mechanisms upon the population of the S1(ππ* La) state …

Field Research Report: Results From The Enreec Vri Field For The 2021, 2022, And 2023 Crop Seasons, Derek M. Heeren, Ali T. Mohammed, Eric Wilkening, Christopher M. U. Neale, Alan L. Boldt, Ankit Chandra, Precious Nneka Amori, Ivo Z. Goncalves, Yeyin Shi, Guillermo R. Balboa

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Long-term irrigation management research has been conducted from 2014 to 2023 for corn and soybean at the Eastern Nebraska Research, Extension, and Education Center (ENREEC) Variable Rate Irrigation (VRI) Field located in subhumid east-central Nebraska (in the Lower Platte North Natural Resources District). The objective of this report was to present the overall results from the VRI Field for 2021 to 2023. Across the three growing seasons, there were the following irrigation treatments: Best Management Practice (BMP), 50% BMP, 125% BMP, rainfed, Spatial ET Modeling Interface (SETMI), SDD1, SDD2, machine-learning-based Cyber-Physical System (CPS), a student team recommended rate, and industry …

Development Of Mass Spectrometric Glycan Characterization Tags Using Acid-Base Chemistry And/Or Free Radical Chemistry, Rayan Murtada, Shane Finn, Jinshan Gao

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Despite recent advances in glycomics, glycan characterization still remains an analytical challenge. Accordingly, numerous glycan-tagging reagents with different chemistries were developed, including those involving acid-base chemistry and/or free radical chemistry. Acid-base chemistry excels at dissociating glycans into their constituent components in a systematic and predictable manner to generate cleavages at glycosidic bonds. Glycans are also highly susceptible to depolymerization by free radical processes, which is supported by results observed from electron-activated dissociation techniques. Therefore, the free radical activated glycan sequencing (FRAGS) reagent was developed so as to possess the characteristics of both acid-base and free radical chemistry, thus generating information-rich …

Automated Tree Mortality Detection Using Ubiquitously Available Public Data, Michael T. Huggins

Master's Theses

Understanding the dynamic interplay between fire severity, topography, and tree mortality, is crucial for predicting future forest dynamics and enhancing resilience against climate change-induced wildfire regimes. This thesis develops a multi-sensor approach for automated estimation of tree mortality, then applies it to examine trends in tree mortality over a six-year period across a fire affected study site in the Trinity River basin in Northern California. The Random Forest model uses publicly available USGS 3D Elevation Program Lidar (3DEP) and NAIP imagery as inputs and is likely to be easily adaptable to other landscapes. The model had a Receiver Operating Characteristic …

Existence And Uniqueness Of Solutions Of Sobolev Type Second Order Integrodifferential Equation, Kamalendra Kumar, Manish Nath Tripathi

Applications and Applied Mathematics: An International Journal (AAM)

The primary concern of this article is to establish the existence, uniqueness and continuous dependence on initial data of mild solutions of second order mixed integrodifferential equations of Sobolev type in Banach spaces. For this objective, we employ the idea of strongly continuous cosine family of operators, the modified version of Banach theorem and Grownwall’s inequality. The model is demonstrated to elucidate the abstract conclusion.

Some Generalizations Of Corona Product Of Two Graphs, Aparajita Borah, Gajendra Pratap Singh

Applications and Applied Mathematics: An International Journal (AAM)

In this paper we are seeking to conceptualize the notion of corona product of two graphs to contrive some special types of graphs. That is, here our attempt is to regenerate a familiar graph as a product graph. We are considering seven familiar graphs here to reconstruct them with the help of corona product of two graphs. Such types of families of the graphs and operations can be used to study biological pathways as well as to find the optimal order and size for the special types of graphs.

Forging The Future: Strategic Approaches To Quantum Ai Integration For Industry Transformation, Meng Leong How, Sin Mei Cheah

CCX Research

The fusion of quantum computing and artificial intelligence (AI) heralds a transformative era for Industry 4.0, offering unprecedented capabilities and challenges. This paper delves into the intricacies of quantum AI, its potential impact on Industry 4.0, and the necessary change management and innovation strategies for seamless integration. Drawing from theoretical insights and real-world case studies, we explore the current landscape of quantum AI, its foreseeable influence, and the implications for organizational strategy. We further expound on traditional change management tactics, emphasizing the importance of continuous learning, ecosystem collaborations, and proactive approaches. By examining successful and failed quantum AI implementations, lessons …

Meta-Interpretive Learning With Reuse, Rong Wang, Jun Sun, Cong Tian, Zhenhua Duan

Research Collection School Of Computing and Information Systems

Inductive Logic Programming (ILP) is a research field at the intersection between machine learning and logic programming, focusing on developing a formal framework for inductively learning relational descriptions in the form of logic programs from examples and background knowledge. As an emerging method of ILP, Meta-Interpretive Learning (MIL) leverages the specialization of a set of higher-order metarules to learn logic programs. In MIL, the input includes a set of examples, background knowledge, and a set of metarules, while the output is a logic program. MIL executes a depth-first traversal search, where its program search space expands polynomially with the number …

Health And Safety Plan (Hasp) Butte Priority Soils Operable Unit, Woodard & Curran

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Single Domain Soft Ferromagnetic Ferrofluid Suitable For Intratumoural Magnetic Hyperthermia, Karthickraja Duraisamy, Muthu Devaraj, Ajithkumar Gangadharan, Karen S. Martirosyan, Niroj Kumar Sahu, Prasath Manogaran, Girija Easwaradas Kreedapathy

Physics & Astronomy Faculty Publications

Magnetic hyperthermia is an effective modality for treating cancerous cells selectively at the intracellular level via proper administration, surface tagging and appropriate choice of magnetic nanoparticle. Also, achieving the therapeutic temperature with as much low concentration of nanoparticles, as possible, is desirable. Recently, ferromagnetic nanoparticles or ferrofluid are gaining attention as effective magnetic hyperthermia agents due to their enhanced heating potential under in vivo conditions. In the present work, we have fabricated citrate capped cobalt ferrite ferrofluid via a simple two-step process and investigated the physicochemical, magnetic and induction heating performance. The average size of the synthesized nanoparticle …

Final Quarterly Operations And Maintenance Report Butte Treatment Lagoon System – First Quarter 2022, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Stage And Discharge Prediction From Documentary Time-Lapse Imagery, Kenneth W. Chapman, Troy E. Gilmore, Mehrube Mehrubeoglu, Christian D. Chapman, Aaron R. Mittelstet, John E. Stranzl Jr.

Conservation and Survey Division: Faculty and Staff Publications

Imagery from fixed, ground-based cameras is rich in qualitative and quantitative information that can improve stream discharge monitoring. For instance, time-lapse imagery may be valuable for filling data gaps when sensors fail and/or during lapses in funding for monitoring programs. In this study, we used a large image archive (>40,000 images from 2012 to 2019) from a fixed, ground-based camera that is part of a documentary watershed imaging project (https://plattebasintimelapse.com/). Scalar image features were extracted from daylight images taken at one-hour intervals. The image features were fused with United States Geological Survey stage and discharge data as response variables …

First Egg Dates Of Tree Swallows (Tachycineta Bicolor) Nesting In Boxes In West Michigan Advanced With Increasing Spring Temperatures Between 1993 And 2018, Michael P. Lombardo, Nathaniel Wagner, Jared M. Laughlin, Sango Otieno, Levi Rosendall, Clara Voetberg, Alejandro Hoban

Open Access Publishing Support Funded Articles

Many bird species have advanced their first egg dates in response to recent milder winters and increases in spring temperatures. Tree Swallows (Tachycineta bicolor) begin egg laying earlier in warmer springs at most, but not all, study sites. We examined whether Tree Swallows that nested in boxes in west Michigan responded to an increase in spring temperatures from 1993 to 2018 by advancing their first egg dates. May, but not April, mean daily temperatures got significantly warmer from 1993 to 2018. We found that the first egg dates, but not mean first egg dates, of both second year …

A 2,000-Year Record Of Eelgrass (Zostera Marina L.) Colonization Shows Substantial Gains In Blue Carbon Storage And Nutrient Retention, Martin Dahl, Martin Gullström, Irene Bernabeu, Oscar Serrano, Carmen Leiva-Dueñas, Hans W. Linderholm, Maria E. Asplund, Mats Björk, Tinghai Ou, J. Robin Svensson, Elinor Andrén, Thomas Andrén, Sanne Bergman, Sara Braun, Anneli Eklöf, Zilvinas Ežerinskis, Andrius Garbaras, Petter Hällberg, Elin Löfgren, Malin E. Kylander, Pere Masqué, Justina Šapolaitė, Rienk Smittenberg, Miguel A. Mateo

Research outputs 2022 to 2026

Assessing historical environmental conditions linked to habitat colonization is important for understanding long-term resilience and improving conservation and restoration efforts. Such information is lacking for the seagrass Zostera marina, an important foundation species across cold-temperate coastal areas of the Northern Hemisphere. Here, we reconstructed environmental conditions during the last 14,000 years from sediment cores in two eelgrass (Z. marina) meadows along the Swedish west coast, with the main aims to identify the time frame of seagrass colonization and describe subsequent biogeochemical changes following establishment. Based on vegetation proxies (lipid biomarkers), eelgrass colonization occurred about 2,000 years ago after geomorphological changes …

Introducing Pour Points: Characteristics And Hydrological Significance Of A Rainfall-Concentrating Mechanism In A Water-Limited Woodland Ecosystem, Ashvath S. Kunadi, Tim Lardner, Richard P. Silberstein, Matthias Leopold, Nik Callow, Erik Veneklaas, Aryan Puri, Eleanor Sydney, Sally E. Thompson

Research outputs 2022 to 2026

The interception of rainfall by plant canopies alters the depth and spatial distribution of water arriving at the soil surface, and thus the location, volume, and depth of infiltration. Mechanisms like stemflow are known to concentrate rainfall and route it deep into the soil, yet other mechanisms of flow concentration are poorly understood. This study characterizes pour points, formed by the detachment of water flowing under a branch, using a combination of field observations in Western Australian banksia woodlands and rainfall simulation experiments on Banksia menziesii branches. We aim to establish the hydrological significance of pour points in a water-limited …

Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas

Department of Entomology: Dissertations, Theses, and Student Research

Pollinators are intrinsically linked to the success of unmanaged and managed ecosystems by providing pollination services that aid in the reproduction of wildflowers and many crops. Land use change, habitat loss, fragmentation, and related landscape-level phenomena (for example, increased pesticide exposure) threaten pollinators and have been associated with population declines. In the Midwestern region of the United States, land conversion of native prairies and grasslands to row-crop agriculture has been one of the largest contributors to pollinator habitat loss. Conservation programs, such as the Conservation Reserve Program, have worked towards removing environmentally sensitive lands from agriculture production and enrolling them …

Weakly Supervised Attention-Based Recognition Under Spectral, Turbulence, And Resource Variations, Kshitij Naresh Nikhal

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

While supervised optimization paradigms are ubiquitous across diverse recognition systems, the risk of over-fitting and increasing bias have limited their applicability.

This dissertation focuses on unsupervised learning—learning without precisely curated data—and argues that unsupervised learning methods can enable both discriminability and generalizability. Through the use of attention-based machine learning and advanced clustering, unsupervised methods are able to focus on fine-grained information in images without any explicit supervision. The dissertation introduces a domain-bridging framework for tasks like cross-spectrum matching and long-range recognition, utilizing intra-domain clustering and inter-domain matching to generate pseudo-labels. Additionally, a hash-based network is proposed to accelerate the search …

Water Quality Assessment For San Luis Obispo Creek And Stenner Creek, Brent Flemmer

Master of Science in Environmental Sciences and Management Projects

Water quality was assessed in five stream segments in San Luis Obispo and Stenner Creeks in Spring of 2023. Two of the five sites were located along San Luis Obispo Creek, and three located along Stenner Creek. Criteria to evaluate these aquatic systems was provided by the Standard Operating Procedures of the Surface Water Ambient Monitoring Program, formulated by the State Water Resources Control Board. These procedures describe the methodology surrounding the collection of chemical, physical, and biological parameters of stream systems; streamflow, substrate, flow habitat, chemical water quality, and benthic macroinvertebrates are some of the criteria collected that allows …

Utilization Of Caputo Fractional Derivative In Mhd Nanofluid Flow With Soret And Thermal Radiation Effects, Harshad Patel, Gopal Nanda

Applications and Applied Mathematics: An International Journal (AAM)

In existence of heat diffusion and thermal radiation, an analytical equation is found for unsteady MHD flow past an exponentially accelerating vertical plate in optically thick water based nanofluid. The governing equations are made dimensionless by similarity transformation. A definition of Caputo fractional derivative is applied to generalize governing system of partial differential equations. Laplace transform techniques are applied and obtained the analytical solutions of proposed problems. For a physical point of view, numerical results are obtained using MATLAB software and presented via graphs. From the results, it is concluded that magnetic fields tend to reduce velocity. It is also …