Digital Commons Network^™

Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood

IGC Proceedings (1977-2023)

An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.

Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein

IGC Proceedings (1977-2023)

Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.

A Revised Method Of Condensed Tannin Analysis In Leucaena Spp., S A. Dalzell, G L. Kerven

IGC Proceedings (1977-2023)

The proanthocyanidin (butanol-HCl) assay was used to measure condensed tannin (CT) in extracts from Leucaena spp. at the University of Queensland. Inconsistent results were found to be caused by the iron catalyst in the butanol/HCl reagent and the presence of ascorbic acid in the sample matrix which enhanced anthocyanidin development. In addition, preparation of sample extracts by back extraction with organic solvents reduced CT recoveries. An accurate and rapid technique was developed that measures CT directly in 70% aqueous acetone 0.1% (w/v) sodium metabisulphite plant extracts.

The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka

IGC Proceedings (1977-2023)

Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …

The Vulnerabilities Of Artificial Intelligence Models And Potential Defenses, Felix Iov

Cybersecurity Undergraduate Research Showcase

The rapid integration of artificial intelligence (AI) into various commercial products has raised concerns about the security risks posed by adversarial attacks. These attacks manipulate input data to disrupt the functioning of AI models, potentially leading to severe consequences such as self-driving car crashes, financial losses, or data breaches. We will explore neural networks, their weaknesses, and potential defenses. We will discuss adversarial attacks including data poisoning, backdoor attacks, evasion attacks, and prompt injection. Then, we will explore defense strategies such as data protection, input sanitization, and adversarial training. By understanding how adversarial attacks work and the defenses against them, …

Rollins Oral History Interview With Bruce Stephenson, Bruce Stephenson, Wenxian Zhang

Oral Histories

Bruce Stephenson earned a BA in history from Florida Southern College and a Master in city planning from Ohio State University.  He worked as land use planner for Pinellas County, Florida before receiving a PhD from Emory University.  His dissertation unveiled John Nolen’s 1923 plan for St. Petersburg, Florida’s first comprehensive city plan, and centered Stephenson's first book, Visions of Eden.

Dr. Stephenson started his teaching career at Rollins in 1988 and served as a professor in Environmental Studies for more than 35 years. His second book, John Nolen, Landscape Architect and City Planner, earned the JB Jackson …

Excellence Summit 2024, Office Of Research And Sponsored Projects Operations

Excellence Summit (Research Week) Archived Proceedings

In it's 17th year, the University of the Incarnate Word, welcome all to read and review the proceedings of the annual research week. The 2024 Excellence Summit highlighted academic excellence across all schools featuring topics from nutrition studies to soil amendments, artificial intelligence, medical case studies, and music composition studies. The rich mixture of humanities, health sciences, physical sciences, education, and business can be found in this booklet. We thank all that contributed to these works and hope the community enjoys learning from today's and tomorrow's leaders in research, inquiry, and scholarship.

Ensuring Clean Futures: The Vital Role Of Wastewater Treatment, Nadya Casanova

Goal 6: Clean Water and Sanitation

No abstract provided.

Uni Introduction To High School Openscied Workshop [Flier] April 13, 2024, University Of Northern Iowa Science Education Program.

Science Education Update Conference Documents

The flier for the Introduction to High School OpenSciEd Workshop held on April 13, 2024 at the University of Northern Iowa.

Cpm-8 Europa Unveiled: A Multi-Wavelength Analysis, Trinity A. Ward, Michelle Deady

SC Upstate Research Symposium

This study is an analysis of the compositional structure of Jupiter’s icy moon, Europa, by utilizing multi-wavelength spectroscopy. The objective of this study is to enhance our understanding of Europa’s compositional and potential biological characteristics.

Spectral data was obtained from various space missions and instruments. We are looking at imaging and spectroscopy-focused instruments RALPH, LORRI, LEISA, ALICE, and MVIC which were all part of the New Horizons spacecraft that launched in 2006. The data collected was from the Jupiter fly-by in February-March 2007.

This project sets the tone for the year with the upcoming Europa Clipper mission by NASA, set …

Hgs-3 The Influence Of A Tandem Cycling Program In The Community On Physical And Functional Health, Therapeutic Bonds, And Quality Of Life For Individuals And Care Partners Coping With Parkinson’S Disease, Leila Djerdjour, Jennifer L. Trilk

SC Upstate Research Symposium

Purpose Statement: Several studies have shown that aerobic exercise can have a positive impact on alleviating symptoms experienced by individuals with Parkinson's disease (PD). Despite this evidence, the potential benefits of exercise for both PD patients and their care partners (PD dyad) remain unexplored. This research project investigates the effectiveness, therapeutic collaborations, and physical outcomes of a virtual reality (VR) tandem cycling program specifically designed for PD dyads.

Methods: Following approval from the Prisma Health Institutional Review Board, individuals with PD were identified and screened by clinical neurologists. The pre-testing measures for PD dyads (N=9) included emotional and cognitive status …

Towards Erasing The Distinction Between The Computational And Syntactic Accounts Of Scientific Theories, Timothy Luft

Theses

One of the main goals of philosophy of science is to give a proper account of scientific theories and their structure. One way that accounts of the structure of scientific theories can be distinguished is by the mathematical or logical structures that they involve. For instance, syntactic accounts of scientific theories hold that theories are axioms in a logical framework, whereas semantic accounts are more liberal in the range of mathematical and logical structures they take as pertinent to the structure of scientific theories. Paul Thagard (1988) offers a computational account of scientific theories, which holds that theories are complex …

New Vertebrate Microfossils Expand The Diversity Of The Chondrichthyan And Actinopterygian Fauna Of The Maastrichtian–Danian Hornerstown Formation In New Jersey, Zachary Boles, Paul V. Ullmann, Ian Putnam, Mariele Ford, Joseph T. Deckhut

School of Earth & Environment Departmental Research

The abundance of shark and actinopterygian fossils in the Cretaceous and Paleogene strata of the Atlantic Coastal Plain is well documented; but much remains unknown about the survivorship patterns of these major components of shallow marine faunas in the western Atlantic through the K/Pg mass extinction. To shed light on this subject, we describe an assemblage of new actinopterygian, chondrichthyan, and reptilian microfossils recently recovered from the Maastrichtian Navesink and Maastrichtian–Danian Hornerstown formations at the Jean and Ric Edelman Fossil Park at Rowan University in Mantua Township, New Jersey. The new microfossils clarify extinction patterns across the K/Pg, create temporal …

Role Of Intermolecular Interactions Between Nanoparticle Capping Ligands And Hydrogel Surfaces During Sliding, Lauren Buckley, Connor Bovia, Griffin Gleeson

23rd Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2024)

Modern treatments for osteoarthritis increasingly involve the use of nanoparticles as drugdelivery systems, but there is little known about the influence of nanoparticle chemical composition and surface chemistry between nanoparticles and soft materials in sliding contact. This work implements cartilage-mimicking polyacrylamide (PAM) hydrogels as a well-studied, fundamental platform. In situ (in a fluid environment) macroscale friction tests as a function of shear rate were conducted with a rheometer with a tribology adapter, controlling for contact pressure. Comparing different nanoparticle compositions, citrate capped metal (gold) nanoparticles exhibited a 50% increase in friction relative to water. With no difference in solution viscosity, …

Structure Of Native Four-Repeat Satellite Iii Sequence With Non-Canonical Base Interactions, Erin Chen , '23, M. Trajkovski, Hyun Kyung Lee , '21, Samantha Nyovanie, Kailey N. Martin, W. L. Dean, M. Tahiliani, J. Plavec, Liliya A. Yatsunyk

Chemistry & Biochemistry Faculty Works

Tandem-repetitive DNA (where two or more DNA bases are repeated numerous times) can adopt non-canonical secondary structures. Many of these structures are implicated in important biological processes. Human Satellite III (HSat3) is enriched for tandem repeats of the sequence ATGGA and is located in pericentromeric heterochromatin in many human chromosomes. Here, we investigate the secondary structure of the four-repeat HSat3 sequence 5′-ATGGA ATGGA ATGGA ATGGA-3′ using X-ray crystallography, NMR, and biophysical methods. Circular dichroism spectroscopy, thermal stability, native PAGE, and analytical ultracentrifugation indicate that this sequence folds into a monomolecular hairpin with non-canonical base pairing and B-DNA characteristics at concentrations …

Understanding The Mechanism Behind Hfip's Effect On Diastereomeric Separation Of Oligonucleotides, Ian Neidigh

Honors Theses

The goal of this research was to determine the mechanism behind 1,1,1,3,3,3-hexafluoroisopropanol's (HFIP0 ability to suppress the separation of oligonucleotide diastereomers. This was accomplished by selecting different molecules that were like HFIP but differed in specific ways such as polarity, number and type of halogen(s), alcohol group location, carbon chain length, hydrophobicity, and chao/kosmotropicity. These molecules replaced HFIP in an IPRP chromatography method to determine if splitting of the diastereomers was observed and then they would becompared to HFIP's effect. Viscosity tests were to run to acquire quantitative data to determine chao/kosmotropicity. No decisive trend among a single factor was …

Phun With Phages: Discovering Novel Bacteriophages In The Soil, Julia Kutz, Ryan Dodds, Logan Dykstra, Taylor M. Rentschler, Emma Steinhardt, Megan Vangarven, Jacie Vanderwaal, Shayler Vangelder, Byron Noordewier, Sara S. Tolsma

Celebration of Research

We used three bacterial hosts: Mycobacterium smegmatis, Microbacterium foliorum, and Gordonia terrae, to isolate novel bacteriophages from soil samples. We named these phages, created high titer lysates, and purified their DNA genomes. We have archived the high titer lysates at Northwestern College and the University of Pittsburgh. The genomes of three of our phages were sequences at the University of Pittsburgh and we will be sequencing the remaining genomes this summer. Additionally, we are planning to image our phages with transmission electron microscopy at the University of Iowa or Nebraska yet this semester.

Patterns, Drivers, And Implications Of Postfire Delayed Tree Mortality In Temperate Conifer Forests Of The Western United States, Sebastian Busby, Cody Evers, Andrés Holz

Geography Faculty Publications and Presentations

Conifer forest resilience may be threatened by increasing wildfire activity and compound disturbances in western North America. Fire refugia enhance forest resilience, yet may decline over time due to delayed mortality—a process that remains poorly understood at landscape and regional scales. To address this uncertainty, we used high-resolution satellite imagery (5-m pixel) to map and quantify delayed mortality of conifer tree cover between 1 and 5 years postfire, across 30 large wildfires that burned within three montane ecoregions in the western United States. We used statistical models to explore the influence of burn severity, topography, soils, and climate moisture deficit …

Shared Genomic Segments Analysis Identifies Mhc Class I And Class Iii Molecules As Genetic Risk Factors For Juvenile Idiopathic Arthritis, Cecile N. Avery, Nicole D. Russell, Cody J. Steely, Aimee O. Hersh, John F. Bohnsack, Sampath Prahalad, Lynn B. Jorde

Markey Cancer Center Faculty Publications

Juvenile idiopathic arthritis (JIA) is a complex rheumatic disease encompassing several clinically defined subtypes of varying severity. The etiology of JIA remains largely unknown, but genome-wide association studies (GWASs) have identified up to 22 genes associated with JIA susceptibility, including a well-established association with HLA-DRB1. Continued investigation of heritable risk factors has been hindered by disease heterogeneity and low disease prevalence. In this study, we utilized shared genomic segments (SGS) analysis on whole-genome sequencing of 40 cases from 12 multi-generational pedigrees significantly enriched for JIA. Subsets of cases are connected by a common ancestor in large extended pedigrees, increasing the …

Variable Drought Impacts On Stream Habitat Across The Northwestern Us, Sophia Withers

Student Research Symposium

The United States has been in a continuous drought since 2002. Stream quality, specifically in the context of fish habitat, depends on many variables, including vegetation quality. Drought conditions affect vegetation conditions, however it is unclear how drought conditions impact stream habitat quality for fish. Objectives for my project include:

• How does stream habitat quality relate to drought across National Forests of the Northwestern US?
• Has drought increased impacts on riparian vegetation over the past 20 years?

Chemtrails: Deadly Skies Or Dangerous Misinformation?, Abigail Foust

Undergraduate Research Symposium

A common sight on a clear day is a thin strip of white across the otherwise blue sky: A contrail. Most people do not think anything of these contrails, and assume they have little to no impact on the world at large. Suppose for a moment, however, that they are not as harmless as some believe. Suppose these cloud trails are actually composed of dangerous chemicals, and are being used by sinister forces in a conspiracy to kill off the weak, mind-control the population, or simply dull our minds in preparation for a government takeover. As far-fetched as this may …

Effects Of Reproduction On Senescence In Local Species, Nathan Smith

Undergraduate Research Conference at Missouri S&T

Understanding the impact of reproduction on the aging process within species populations remains an ongoing challenge in ecological and evolutionary research. In this study, we aim to clarify the relationship between reproduction and senescence using agent-based modeling using wild species data sets. Our objectives include investigating how variations in reproductive rates influence the lifespan and aging trajectories of individuals within populations, as well as identifying potential mechanisms underlying these effects. We will employ agent-based modeling to simulate populations and explore the dynamics of reproduction and senescence using publicly available datasets of local wild species. By manipulating parameters related to reproductive …

Effect Of Morphology As A Function Of The K-Index On The Drug Loading And Release Profiles In Polyurea And Poly(Isocyanurate-Urethane) Aerogels, Kamden J. George, Stephen Y. Owusu, Chariklia Sotiriou-Leventis

Undergraduate Research Conference at Missouri S&T

This study explores the potential to predict the drug loading and release profiles of aerogels based on their morphologies: a milestone in drug delivery research which can help save time and cost invested in formulating new aerogel drug carriers and cut-down evaluation of the drug delivery capabilities of aerogels to a few experimental runs. Polyurea (PUA) and poly (isocyanurate-urethane) (PIR-PUR) aerogels were used as model systems while 5-fluorouracil (5-FU) and paracetamol (PM) were used as model drugs. These model systems were chosen because they can be synthesized into different morphologies identified by the so-called K-index (contact angle:porosity). The model drugs …

Simulating Inter-Species Competition In C. Elegans, Kevin Lai

Undergraduate Research Conference at Missouri S&T

In biological research, understanding the life cycles of Caenorhabditis elegans (C. elegans) is pivotal for insights into developmental biology, genetics, and population dynamics. Our project builds on Worm-Pop, a Python-based multi-agent simulation of Caenorhabditis elegans (C. elegans) , to enhance its capabilities in simulating survival strategies, reproductive success, and genetic drift. The current model simulates a uniform population without inter-agent interactions. I plan to introduce multiple species of worms into the simulation to study competitive dynamics and determine which variants are most successful under various conditions. Pheromones significantly influence C. elegans behavior, affecting mating, foraging, and social interactions. To address …

Cytokinesis And Dbf2, Katharine Gray, Katie Shannon

Undergraduate Research Conference at Missouri S&T

During cell division, the cytoplasm must be divided between the parent and daughter cell. The Mitotic Exit Network (MEN) is a signaling pathway that allows a dividing cell to exit mitosis and complete cytokinesis. Obf2 is a MEN protein kinase in yeast, which provides a simplified model for studying this conserved pathway. By regulating Dbf2s' activity through phosphorylation mutants, its effect on cytokinesis can be studied. Cells were viewed under a microscope, to visualize division errors. Yeast cells that fail to complete cytokinesis will form chains, as the budding daughter cells fail to separate from their parents. Chains of 3 …

Computational Modeling Of Retinal Damage Thresholds, Payton Hoffman, Eos Shapland, Alan Enriquez

SACAD: Scholarly Activities

The Scalable Effects Simulation Environment(SESE), is the computational Biophysics software contracted by Nanohmics we used for our research. The goal was to find the minimum energy necessary to damage the retina from 2 laser sources, a 532 nm He-Ne source, and Supercontinuum Laser(SCL) source at 400-1400nm. We needed a 10 times damage ratio for significance. Our data did not show this trend.

Killing Cancer: Manipulating Hydrophobic Vanadium Complexes To Improve Anti-Cancer Activity, Levi Ausherman, Debbie C. Crans, Peter A. Lay, Maggi Braasch-Turi

SACAD: Scholarly Activities

Hydrophobic vanadium complexes have recently shown improved anti-cancer activities compared to cisplatin. The hydrophobicity and anti-proliferative activity of [VO(Hshed)(dtb)] ([Hshed= N-(salicylideneaminato)-N’-(2-hydroxyethyl)-1,2-ethanediamine and dtb= 3,5-di(tert-butyl)catechol)]) have inspired the development of a library of hydrophobic vanadium complexes. Increasing the steric bulk of the catechol ligand has been shown to have a direct impact on hydrophobicity and anti-proliferative activities. Currently at Fort Hays State University, the Braasch-Turi group is synthesizing VO(HSHED)(dtb) to build up material to support the chemical analysis and biological assay performed by our collaborators at Colorado State University and the University of Sydney, Australia, respectively. In the future, we plan …

Genomic Population Structure Of Great Hammerhead Sharks (Sphyrna Mokarran) Across The Indo-Pacific, Naomi L. Brunjes, Samuel M. Williams, Alexis L. Levengood, Matt K. Broadhurst, Vincent Raoult, Alastair V. Harry, Matias Braccini, Madeline E. Green, Julia L. Y. Spaet, Michael J. Travers, Bonnie J. Holmes

Fisheries Research Articles

Context

Currently, little information exists describing the population structure of great hammerhead sharks (Sphyrna mokarran) in Australian waters. Aims

This study used single nucleotide polymorphisms to investigate fine-scale population structure in S. mokarran across the Indo-Pacific. Methods

DNA was extracted from 235 individuals across six Australian locations and a Red Sea outgroup. Population parameters were calculated and visualised to test structuring across locations. Key results

No fine-scale population structuring was observed for S. mokarran across the Indo-Pacific. However, population structuring occurred for all Australian locations when compared to the Red Sea outgroup. Conclusions

Findings suggest a single stock …

Ultrasoft Platelet-Like Particles Stop Bleeding In Rodent And Porcine Models Of Trauma, Kimberly Nellenbach, Emily Mihalko, Seema Nandi, Drew W. Koch, Jagathpala Shetty, Leandra Moretti, Jennifer Sollinger, Nina Moiseiwitsch, Ana Sheridan, Sanika Pandit, Maureane Hoffman, Lauren V. Schnabel, L. Andrew Lyon, Thomas H. Barker, Ashley C. Brown

Engineering Faculty Articles and Research

Uncontrolled bleeding after trauma represents a substantial clinical problem. The current standard of care to treat bleeding after trauma is transfusion of blood products including platelets; however, donated platelets have a short shelf life, are in limited supply, and carry immunogenicity and contamination risks. Consequently, there is a critical need to develop hemostatic platelet alternatives. To this end, we developed synthetic platelet-like particles (PLPs), formulated by functionalizing highly deformable microgel particles composed of ultralow cross-linked poly (N-isopropylacrylamide) with fibrin-binding ligands. The fibrin-binding ligand was designed to target to wound sites, and the cross-linking of fibrin polymers was designed …

Developmental Characteristics And Renovating Pattern Of Reed Canarygrass Shoots In A Sward, M Ito, S Mizuno, T Otani, S Kobayashi

IGC Proceedings (1977-2023)

Developmental and renovating traits of shoots were investigated ecomorphologically in a reed canarygrass (Phalaris arundinacea L., cv. Palaton) sward harvested 3 times per year. Reed canarygrass shoots exhibited notably high growth rates both in the 1st and 2nd crops together with rapid leaf formation (around 7 days/leaf) and active internode elongation. Vigorously growing shoots, which regenerated soon after the 1st cutting and dominated in the 2nd crop canopy, mainly originated from the stubble formed with 3 to 4 phytomers that developed after the beginning of spring growth, thus resulting in higher shoot density than that of the 1st crop. …