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Articles 68881 - 68910 of 79063

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Measures Of Denitrification In Selected South Dakota Semi-Permanent Prairie Pothole Wetlands, Beverly S. Klein Jan 2001

Measures Of Denitrification In Selected South Dakota Semi-Permanent Prairie Pothole Wetlands, Beverly S. Klein

Electronic Theses and Dissertations

Wetlands are an integral part of agricultural systems in the prairie pothole regions of the North Central United States and Canadian Provinces. Little research has been done on denitrification in prairie potholes, and a better understanding of their denitrifying capability could aide in optimizing management practices near pothole areas. Most probable number (MPN) and denitrifying enzyme activity (DEA or Phase I) assays were conducted. Most probable number (MPN) measures were used to give an estimate of dissimilatory nitrate reduction to ammonia (DNRA) and denitrifying populations present in the wetlands. This study involved 3 selected semi-permanent prairie pothole wetlands near Madison, …

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Invertebrate Egg And Plant Seed Banks In Natural, Restored, And Drained Wetlands In The Prairie Pothole Region (Usa) And Potential Effects Of Sedimentation On Recolonization Of Hydrophytes And Aquatic Invertebrates, Robert Andrew Gleason Jan 2001

Invertebrate Egg And Plant Seed Banks In Natural, Restored, And Drained Wetlands In The Prairie Pothole Region (Usa) And Potential Effects Of Sedimentation On Recolonization Of Hydrophytes And Aquatic Invertebrates, Robert Andrew Gleason

Electronic Theses and Dissertations

Sediment is the major pollutant of wetlands, lakes, rivers, and estuaries in the United States and it poses unique threats to wetlands of the Prairie Pothole Region (PPR). Sediment may impact the success of wetland restorations because burial of invertebrate and plant propagules may impact hatching and germination success, and hence, may hamper successional changes throughout interannual climate cycles. Sedimentation also reduces the pool depth and volume, further exacerbating the recovery of hydrophyte communities in restored wetlands. I evaluated the potential impacts of sedimentation on prairie wetlands from several perspectives. First, I evaluated the effects of sedimentation on loss of …

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Durango Hills Water Resource Center, City Of Las Vegas, Nevada Jan 2001

Durango Hills Water Resource Center, City Of Las Vegas, Nevada

Publications (WR)

No abstract provided.

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Environmental Analysis Of Cores From The Helike Delta, Gulf Of Corinth, Greece, S. Soter, Patricia Blackwelder, C. Tziavos, D. Katsonopoulou, Terri Hood, Carlos A. Alvarez Zarikian Jan 2001

Environmental Analysis Of Cores From The Helike Delta, Gulf Of Corinth, Greece, S. Soter, Patricia Blackwelder, C. Tziavos, D. Katsonopoulou, Terri Hood, Carlos A. Alvarez Zarikian

Marine & Environmental Sciences Faculty Articles

The fan delta southeast of Aigion on the southwest shore of the Gulf of Corinth was the site of ancient Helike, a city destroyed and submerged by an earthquake and seismic sea wave in 373 BC. Bore holes drilled on the Helike Delta yielded numerous ceramic fragments in the upper 12 meters, and a record of changing local environments on the delta during the Holocene period. At about 8 m below present sea level the core profiles show a general upward transition from marine to lacustrine/lagoonalc onditions.T he transition dates from about 8 kyr BP and is probably due to …

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Utility Of Walleyes And Saugeyes As Secondary Predators In Small South Dakota Impoundments, Mark J. Ermer Jan 2001

Utility Of Walleyes And Saugeyes As Secondary Predators In Small South Dakota Impoundments, Mark J. Ermer

Electronic Theses and Dissertations

Walleye Stizostedion vitreum and saugeye (walleye x sauger S. canadense) fingerlings were concurrently stocked at equal densities into five small impoundments in 1997, 1988, and 1999 to evaluate relative survival, growth and their ability to restructure existing panfish communities. Prior to stocking, fish were differentially marked on the right or left side with freeze brands or with 700 ppm oxytetracycline hydrochloride (OTC) to allow accurate identification of each fish as a walleye (fry mark) or a saugeye (fingerling mark). Spring and fall night electrofishing was used to sample the stocked percids, as well as potential competing species such as largemouth …

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Biodiversity: Connecting With The Tapestry Of Life, Elise F. Granek, Francisco Dallmeier, Alfonso Alonso, Peter H. Raven Jan 2001

Biodiversity: Connecting With The Tapestry Of Life, Elise F. Granek, Francisco Dallmeier, Alfonso Alonso, Peter H. Raven

Environmental Science and Management Faculty Publications and Presentations

Biodiversity is the extraordinary variety of life on Earth – from genes and species to ecosystems and the valuable functions they perform. E.O. Wilson, the noted biologist and author who coined the term “biodiversity,” explains it as “the very stuff of life.” Species and the ecosystems in which they live are indelibly linked. Conversion or loss of ecosystems inevitably impairs the species that depend on them. As well, changes in the life cycle of one species could impact the life cycles of many other species (including humans), alter ecosystems and ecosystem functions, and contribute to local, regional and, ultimately, global …

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Impedance Feedback Control For Scanning Electrochemical Microscopy, David O. Wipf, Mario A. Apuche-Avlles Jan 2001

Impedance Feedback Control For Scanning Electrochemical Microscopy, David O. Wipf, Mario A. Apuche-Avlles

CAS Publications

A new constant-distance imaging method based on the relationship between tip impedance and tip-substrate separation has been developed for the scanning electrochemical microscope (SECM). The tip impedance is monitored by application of a high frequency ac voltage bias between the tip and auxiliary electrode. The high frequency ac current is easily separated from the dc level faradaic electrochemistry with a simple RC filter, which allows impedance measurements during feedback or generation/collection experiments. By employing a piezo-based feedback controller we are able to maintain the impedance at a constant value and, thus, maintain a constant tip-substrate separation. Application of the method …

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Contents Jan 2001

Contents

Journal of Mathematics and Science: Collaborative Explorations

No abstract provided.

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The Design, Implementation, And Assessment Of A New Capstone Course Aimed At Science Education Majors, R. Betancourt, J. Arce Jan 2001

The Design, Implementation, And Assessment Of A New Capstone Course Aimed At Science Education Majors, R. Betancourt, J. Arce

Journal of Mathematics and Science: Collaborative Explorations

This paper rationalizes the selection of the concept of energy as the central theme of a new capstone course aimed at science education majors. It describes the goals of the course and the activities that preceded the course design and led to the selection of the topics, of the educational materials, and of the teaching methodologies. It presents a sequential description of the manner in which the conceptual knowledge of energy was to be developed. The specific experiments, interactive demonstrations and other educational materials utilized for the conceptual development of the concept of energy in context are described and referenced. …

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The Transition To College Process In Pr-Cetp Scholars, E. Rivera, M. Rosa, A. Santiago, N. Torres, M. Bravo Jan 2001

The Transition To College Process In Pr-Cetp Scholars, E. Rivera, M. Rosa, A. Santiago, N. Torres, M. Bravo

Journal of Mathematics and Science: Collaborative Explorations

This article describes a study about the experiences of a group of students during the transition from high school to college. The students are future teachers who evidenced a high level of academic achievement in high school and received merit scholarships from the Puerto Rico Collaborative for Excellence in Teacher Preparation (PR-CETP). Two groups of students were compared: those who sustained a high GPA during their freshman year, and those who did not and, therefore, no longer qualified for the scholarship. The study was carried out through focused interviews with eight students, from three universities, four of whom maintained the …

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Concept Building Through Active Learning Experiences With The Calculator Based Laboratory (Cbl), N. Motta Jan 2001

Concept Building Through Active Learning Experiences With The Calculator Based Laboratory (Cbl), N. Motta

Journal of Mathematics and Science: Collaborative Explorations

No abstract provided.

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Program Evaluation In The Context Of Debates In The Field: The Evaluation Of Pr-Cetp, M. Bravo Jan 2001

Program Evaluation In The Context Of Debates In The Field: The Evaluation Of Pr-Cetp, M. Bravo

Journal of Mathematics and Science: Collaborative Explorations

This paper rationalizes the selection of the concept of energy as the central theme of a new capstone course aimed at science education majors. It describes the goals of the course and the activities that preceded the course design and led to the selection of the topics, of the educational materials, and of the teaching methodologies. It presents a sequential description of the manner in which the conceptual knowledge of energy was to be developed. The specific experiments, interactive demonstrations and other educational materials utilized for the conceptual development of the concept of energy in context are described and referenced. …

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Journal Of Mathematics And Science: Collaborative Explorations Jan 2001

Journal Of Mathematics And Science: Collaborative Explorations

Journal of Mathematics and Science: Collaborative Explorations

No abstract provided.

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Scwds Briefs: Volume 16, Number 4 (January 2001) Jan 2001

Scwds Briefs: Volume 16, Number 4 (January 2001)

Southeastern Cooperative Wildlife Disease Study: Publications


• Avian vacuolar myelinopathy (AVM) was confirmed in eight bald eagles and is suspected in another six eagles that died from mid-November 2000 through late January 2001 at Clarks Hill Lake in eastern Georgia.
• Bovine tuberculosis TB in Manitoba Elk
• CWD in a Nebraska Deer: Chronic wasting disease (CWD) of cervids has been found for the first time in a free-ranging cervid outside Colorado and Wyoming.
• Tropical Bont Tick on St. Croix: The U.S. Department of Agriculture recently announced that the tropical bont tick (Amblyomma variegatum) had been found on St. Croix in the U.S. …

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The Likely Cause Of Extinction Of The Tree Snail Orthalicus Reses Reses (Say), E.A. Forys, A. Quistorff, Craig R. Allen, D.P. Wojcik Jan 2001

The Likely Cause Of Extinction Of The Tree Snail Orthalicus Reses Reses (Say), E.A. Forys, A. Quistorff, Craig R. Allen, D.P. Wojcik

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The Stock Island tree snail, Orthalicus reses reses, went extinct in its native range in the Florida Keys in 1992. Fortunately, O. r. reses has been introduced elsewhere and further reintroductions are currently planned. Before these reintroductions are implemented, it is important to try and determine which factors were most likely to have caused the decline and extinction. While habitat destruction was probably the ultimate reason why there were so few tree snails, it is likely that an interaction of habitat fragmentation and the invasion of an exotic predator caused the final decline that lead to the extinction in …

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The Non-Indigenous Ant, Solenopsis Invicta, Reduces Loggerhead Shrike And Native Insect Abundance, Craig R. Allen, R. Scott Lutz, Tim Lockley, Sherman A. Phillips Jr., Stephen Demarais Jan 2001

The Non-Indigenous Ant, Solenopsis Invicta, Reduces Loggerhead Shrike And Native Insect Abundance, Craig R. Allen, R. Scott Lutz, Tim Lockley, Sherman A. Phillips Jr., Stephen Demarais

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The red imported fire ant (Solenopsis invicta Buren) is an aggressive, non-indigenous species that is a threat to native biota in the southeastern United States. We determined the effect of S. invicta on loggerhead shrike (Lanius ludovicianus L.) abundance and investigated a possible mechanism of impact, which is a reduction in insect prey availability. We used a fire ant bait (hydramethylnon) to reduce fire ant populations on one randomly chosen member of each of five pairs of 202-ha study areas in the Texas coastal Bend region, and also measured shrike relative abundance and a volumetric index of insect …

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Red Imported Fire Ants: Impact On Biodiversity, Daniel P. Wojcik, Craig R. Allen, Richard J. Brenner, Elizabeth A. Forys, Donald P. Jouvenaz, R. Scott Lutz Jan 2001

Red Imported Fire Ants: Impact On Biodiversity, Daniel P. Wojcik, Craig R. Allen, Richard J. Brenner, Elizabeth A. Forys, Donald P. Jouvenaz, R. Scott Lutz

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

The red imported fire ant, Solenopsis invicta Buren (hereafter referred to as imported fire ant), long considered a regional problem, is receiving renewed attention nationwide, with infestations found in Arizona, Maryland, Nevada, New Mexico, and Virginia (Mitchell 1996). Recently, infestations discovered in several regions of California caused great public concern (Brennan 1999, Schrader 1999). Initially, infestations appeared to be isolated in almond groves in Kern County; presumably these infestations originated from bee hives transported interstate for the purpose of pollinating crops. Separately, ornamental plants arriving in Las Vegas, NV, were infested with fire ants, and records showed that the point …

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Inventory Of Parks Division Lands For Threatened And Endangered Species Habitats And Exemplary Natural Communities, Steven B. Rolfsmeier Jan 2001

Inventory Of Parks Division Lands For Threatened And Endangered Species Habitats And Exemplary Natural Communities, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The Nebraska Natural Heritage Program is responsible for environmental review of state projects, including those on Parks division properties, to determine if they may impact state-listed threatened and endangered species. In addition, the Program maintains a statewide database on natural communities and rare and imperiled species. This information is used to inform conservation decision-making, including land management decisions, with a goal of conserving Nebraska’s flora and fauna, our natural legacy. The two main objectives of this inventory project were first to improve the Nebraska Game and Parks Commission’s knowledge of threatened and endangered species and their potential habitat on Parks …

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Remote Sensing Of Biotic Effects: Zebra Mussels (Dreissena Polymorpha) Influence On Water Clarity In Saginaw Bay, Lake Huron, Judith W. Budd, Thomas D. Drummer, Thomas F. Nalepa, Gary L. Fahnenstiel Jan 2001

Remote Sensing Of Biotic Effects: Zebra Mussels (Dreissena Polymorpha) Influence On Water Clarity In Saginaw Bay, Lake Huron, Judith W. Budd, Thomas D. Drummer, Thomas F. Nalepa, Gary L. Fahnenstiel

United States Department of Commerce: Staff Publications

In this study, Advanced Very High Resolution Radiometer (AVHRR) remote sensing reflectance (Rrs), imagery from 1987–1993 is used to study changes in water clarity before and after zebra mussels (Dreissena polymorpha) were discovered in Saginaw Bay, Lake Huron. Spatial and temporal trends in the data indicate distinct and persistent increases in water clarity in the inner bay after the first large recruitment of zebra mussels in the fall of 1991. The pre-Dreissena imagery show that turbidity in the inner bay was influenced by the Saginaw River discharge in spring, biological production (plankton) in summer, and …

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Changes In Diet And Body Condition Of Lake Whitefish In Southern Lake Michigan Associated With Changes In Benthos, Steven A. Pothoven, Thomas F. Nalepa, Philip J. Schneeberger, Stephen B. Brandt Jan 2001

Changes In Diet And Body Condition Of Lake Whitefish In Southern Lake Michigan Associated With Changes In Benthos, Steven A. Pothoven, Thomas F. Nalepa, Philip J. Schneeberger, Stephen B. Brandt

United States Department of Commerce: Staff Publications

We evaluated the long-term trends of the benthic macroinvertebrate community (1980–1999) and biological attributes of lake whitefish Coregonus clupeaformis (1985–1999) in southeastern Lake Michigan. We also determined what food types were important to lake whitefish in an area where the amphipod Diporeia had not yet declined in 1998 and how the diet of lake whitefish changed as Diporeia declined during 1999–2000. Zebra mussels Dreissena polymorpha invaded the study area in 1992; Diporeia began to decline in 1993 and was nearly absent by 1999. The body condition of lake whitefish decreased after 1993 and remained low thereafter. The length at age …

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Cetacean Sightings Around The Republic Of The Maldives, April 1998, Lisa T. Ballance, R. Charles Anderson, Robert L. Pitman, Kathleen Stafford, Abdullah Shaan, Zaha Waheed, Robert L. Brownell Jr. Jan 2001

Cetacean Sightings Around The Republic Of The Maldives, April 1998, Lisa T. Ballance, R. Charles Anderson, Robert L. Pitman, Kathleen Stafford, Abdullah Shaan, Zaha Waheed, Robert L. Brownell Jr.

United States Department of Commerce: Staff Publications

In April 1998, as part of a project to collect biopsy samples of putative pygmy blue whales (Balaenoptera musculus brevicauda) in the waters around the Republic of the Maldives, Indian Ocean, incidental sightings of cetaceans encountered were recorded. Using modified line-transect methods and handheld binoculars, a total of 267 sightings of 16 species of whales and dolphins were recorded during 20 at-sea days in the northeastern part of the atoll. Significant results include the following: (1) cetaceans were abundant and species diversity was high, including nearly every pantropical species of pelagic cetacean; (2) the spinner dolphin (Stenella …

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Recent Declines In Benthic Macroinvertebrate Densities In Lake Ontario, Stephen J. Lozano, Jill V. Scharold, Thomas F. Nalepa Jan 2001

Recent Declines In Benthic Macroinvertebrate Densities In Lake Ontario, Stephen J. Lozano, Jill V. Scharold, Thomas F. Nalepa

United States Department of Commerce: Staff Publications

Surveys of benthic macroinvertebrates conducted in Lake Ontario during 1994 and 1997 revealed recent declines in populations of three major taxonomic groups: Oligochaeta, Sphaeriidae, and Diporeia spp. (Amphipoda), with the most drastic reductions occurring in the latter. Results from sediment measurements were used to classify deepwater sediments into three habitat zones. Densities of all three taxa declined in the shallowest (12–88 m) of the sediment zones between 1994 and 1997; the greatest changes in density were observed for Diporeia, which declined from 3011 to 145 individuals·m–2, and for total benthic macroinvertebrates, which declined from 5831 to 1376 …

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Modeling Reptile And Amphibian Range Distributions From Species Occurrences And Landscape Variables, Geoffrey M. Henebry, Brian C. Putz, James W. Merchant Jan 2001

Modeling Reptile And Amphibian Range Distributions From Species Occurrences And Landscape Variables, Geoffrey M. Henebry, Brian C. Putz, James W. Merchant

United States Geological Survey: Publications

An international symposium in October 1999 demonstrated the state of the art in modeling species occurrences (Scott et al. 2001). One clear message from the symposium was the broad diversity of approaches that constitute the state of the art. No single method excels, largely because of the very particular and local nature of the problem. Organisms both influence and respond to their local environment; thus, the same species may key in on different resources in different landscapes. Furthermore, modeling methods vary widely in their "transparency," which can inhibit transportability or robustness.
In order to provide an analytical modeling framework that …

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Regional Variations In Provenance And Abundance Of Ice-Rafted Clasts In Arctic Ocean Sediments: Implications For The Configuration Of Late Quaternary Oceanic And Atmospheric Circulation In The Arctic, R. L. Phillips, A. Grantz Jan 2001

Regional Variations In Provenance And Abundance Of Ice-Rafted Clasts In Arctic Ocean Sediments: Implications For The Configuration Of Late Quaternary Oceanic And Atmospheric Circulation In The Arctic, R. L. Phillips, A. Grantz

United States Geological Survey: Staff Publications

The composition and distribution of ice-rafted glacial erratics in late Quaternary sediments define the major current systems of the Arctic Ocean and identify two distinct continental sources for the erratics. In the southern Amerasia basin up to 70% of the erratics are dolostones and limestones (the Amerasia suite) that originated in the carbonate-rich Paleozoic terranes of the Canadian Arctic Islands. These clasts reached the Arctic Ocean in glaciers and were ice-rafted to the core sites in the clockwise Beaufort Gyre. The concentration of erratics decreases northward by 98% along the trend of the gyre from southeastern Canada basin to Makarov …

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The Spatial Variability Of Energy And Carbon Dioxide Fluxes At The Floor Of A Deciduous Forest, Kell B. Wilson, Tilden Meyers Jan 2001

The Spatial Variability Of Energy And Carbon Dioxide Fluxes At The Floor Of A Deciduous Forest, Kell B. Wilson, Tilden Meyers

United States Bureau of Land Management: Staff Publications

Fluxes of carbon dioxide, water and sensible heat were measured using three different eddy covariance systems above the forest floor of a closed deciduous forest (leaf area index ~ 6). The primary objective was to examine the representativeness of a single eddy covariance system in estimating soil respiration for time scales ranging from one-half hour to more than one week. Experiments were conducted in which the eddy covariance sensors were in one of three configurations: i) collocated, ii) separated horizontally or iii) separated vertically. A measure of the variation between the three systems (CV' 0, related to the coefficient of …

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Isolation And Characterization Of Chromium- And Cobalt-Binding Peptides, Michael J. Jose Jan 2001

Isolation And Characterization Of Chromium- And Cobalt-Binding Peptides, Michael J. Jose

Honors Theses

Metal substitution has been proposed as an aid in the characterization of the structure of Low-Molecular-Weight Chromium-binding substance (LMWCr), which appears to be the biologically active chromium species. Preparations of bovine liver were diced and suspended in an isolation solution containing either a cobalt or a chromium salt. Peptide samples from these preparations were isolated by precipitation and centrifugation and purified by liquid chromatography. Several methods were used to analyze these samples, which may contain LMWCr or the cobalt-substituted form (LMWCo), in order to determine their purity, molecular weight (MW), and metal-to-peptide ratio. Methods used to determine MW included sodium …

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Accumulation Of Heavy Metals In Freshwater Organisms: Assessment Of Toxic Interactions, Neylan Di̇ri̇lgen Jan 2001

Accumulation Of Heavy Metals In Freshwater Organisms: Assessment Of Toxic Interactions, Neylan Di̇ri̇lgen

Turkish Journal of Chemistry

Heavy metals are continuously released into the terrestrial environment by natural sources and human activities. The uptake and accumulation of heavy metals by plants promotes a mechanistic understanding of the biological significance of particular metal concentrations and distributions in biota. The toxicity of chromium, zinc, copper and cobalt ions and their binary mixtures are studied at varying test levels using duckweed as the test organism. The accumulation of metal ions are determined by atomic absorption spectroscopy. The type of toxic interactions in binary mixtures is assessed as `synergistic', `antagonistic' and `additive' by a statistical approach.

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Synthesis And Characterization Of Copper(Ii), Nickel(Ii), Cadmium(Ii), Cobalt(Ii) And Zinc(Ii) Complexes With 2-Benzoyl-3-Hydroxy-1-Naphthylamino-3-Phenyl-2-Propen-1-On, Mehmet Sönmez Jan 2001

Synthesis And Characterization Of Copper(Ii), Nickel(Ii), Cadmium(Ii), Cobalt(Ii) And Zinc(Ii) Complexes With 2-Benzoyl-3-Hydroxy-1-Naphthylamino-3-Phenyl-2-Propen-1-On, Mehmet Sönmez

Turkish Journal of Chemistry

Cu(II), Ni(II), Co(II), Zn(II) and Cd(II) complexes of 2-Benzoyl-3-hydroxy-1-naphthylamino-3-phenyl-2-propen-1-on, which is a derivative of carboxamide, were prepared and characterized by elemental, spectral and magnetic methods. Based on the analytical results, electronic and infrared spectral data and magnetic susceptibility measurements, suitable structures have been proposed. The formation of the complexes in solution was investigated conductometrically. 2-Benzoyl-3-hydroxy-1-naphthylamino-3-phenyl-2-propen-1-on was found to be a bidentate ligand coordinating through enolic oxygen and amide nitrogen. The complexes of Co(II) and Ni(II) are proposed to have an octahedral structure, while Cd(II), Cu(II) and Zn(II) are suggested to possess a square-planar environment in the complexes.

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The Composition Of Essential Oils From Two Varieties Of Sideritis Erythrantha Var. Erythrantha And Var. Cedretorum, Nurhayat Tabanca, Neş'E Kirimer, K. Hüsnü Can Başer Jan 2001

The Composition Of Essential Oils From Two Varieties Of Sideritis Erythrantha Var. Erythrantha And Var. Cedretorum, Nurhayat Tabanca, Neş'E Kirimer, K. Hüsnü Can Başer

Turkish Journal of Chemistry

Water distilled essential oils from two varieties of Sideritis erythrantha: var. erythrantha} and var. cedretorum, both endemic in Turkey, were analysed by GC/MS. The main components were characterized as \alpha -pinene (16.3-19.5%) and sabinene (6.1- 10.4%) for var. erythrantha; myrcene (21.9-24.3%) and \alpha -pinene (11.4- 12.4%) for var. cedretorum.

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Incorporation Of Edta For The Elimination Of Metal Inhibitory Effects In An Amperometric Biosensor Based On Mushroom Tissue Polyphenol Oxidase, Arzum Erdem, Di̇lşat Özkan, Burcu Meri̇ç, Kağan Kerman, Mehmet Özsöz Jan 2001

Incorporation Of Edta For The Elimination Of Metal Inhibitory Effects In An Amperometric Biosensor Based On Mushroom Tissue Polyphenol Oxidase, Arzum Erdem, Di̇lşat Özkan, Burcu Meri̇ç, Kağan Kerman, Mehmet Özsöz

Turkish Journal of Chemistry

Enzyme based biosensors are highly selective devices which rely on the specific binding of the target analyte (the substrate) to the active-site regions of the enzyme. The enzymatic reaction between mushroom tissue polyphenol oxidase and its substrate phenol is coupled with the use of potassium ferrocyanide (K_{4}Fe(CN)_{6}) as the mediator in ammonia buffer solution (pH 8.80). The response of devices is often affected by the presence of inhibitors, which combine with the free enzyme in a manner that prevents substrate binding. The objective of this study is to describe an efficient and yet simple strategy to eliminate the interference of …

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