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A Comparison Of Unconventional Microwave And Ultrasound-Assisted Extraction Methods Used For Flavonoids, Istiqomah Rahmawati, Daffa Hafiziaulhaq Azizi, Jihan Nafila Wibowo, Muhammad Reza, Boy Arief Fachri, Bekti Palupi, Meta Fitri Rizkiana, Helda Wika Amini, Ifan Ramadana, Felix Arie Setiawan

Makara Journal of Science

Cocoa pods (Theobroma cacao L.) are a rich source of flavonoids, which are natural antioxidants known for their health benefits. This study investigated the use of microwave-assisted extraction (MAE) and ultrasound-assisted ex-traction (UAE) to extract the maximum flavonoids and antioxidants from cocoa pods. MAE and UAE are efficient and sustainable methods for extracting bioactive compounds like flavonoids and antioxidants from cocoa pods, offer-ing faster extraction, reduced solvent use, and better compound preservation compared to conventional methods. These technologies unlock the untapped potential of cocoa pods for applications in food, cosmetics, and pharmaceuti-cals. The effects of extraction time (2–10 min), microwave …

The Binding Of Cu2+ To Lipid Membranes Is Not Substantially Influenced By Electrostatic Screening, Christopher M. Reynolds, Olivia C. Fiebig, Alexa Marroquin, Andrew J. Glaid, Kushaal Chokhany, Ryan G. Zdenek, Maria Laura Da Cruz Garcia, Paul S. Cremer

Chemistry: Faculty Publications

Gouy-Chapman theory predicts that salt screening and modulating the interfacial charge density should strongly influence the apparent dissociation constant, Kd,app, between Cu²⁺ and negatively charged phosphatidylserine (PS) lipids in supported lipid bilayers (SLBs). Specifically, Kd,app would be expected to increase (weaken binding) by a factor of 40 when 100 mM NaCl is introduced into the solution because of electrostatic screening between the membrane and Cu²⁺ cations. Surprisingly, however, fluorescence quenching measurements demonstrate that Kd,app increases by less than a factor of 2 when increasing the salt concentration in the presence of standard buffers, such as tris(hydroxymethyl)aminomethane (Tris). Moreover, …

Overexpression And Biophysical And Functional Characterization Of A Recombinant Fgf21, Phuc Phan, Jason Hoang, Thallapuranam Krishnaswamy Suresh Kumar

Chemistry & Biochemistry Faculty Publications and Presentations

Fibroblast growth factor 21 (FGF21) is an endocrine FGF that plays a vital role in regulating essential metabolic pathways. FGF21 increases glucose uptake by cells, promotes fatty acid oxidation, reduces blood glucose levels, and alleviates metabolic diseases. However, detailed studies on its stability and biophysical characteristics have not been reported. Herein, we present the overexpression, biophysical characterization, and metabolic activity of a soluble recombinant FGF21 (rFGF21). The far-UV circular dichroism spectra of rFGF21 show a negative trough at 215 nm, indicating that the protein's backbone predominantly adopts a 13 sheet conformation. rFGF21 shows intrinsic tyrosine fluorescence at 305 nm. Thermal …

American Option Pricing Using Generalised Stochastic Hybrid Systems, Evelyn Buckwar, Sascha Desmettre, Agnes Mallinger, Amira Meddah

Journal of Stochastic Analysis

No abstract provided.

Energy-Aware Clustering Using Intelligent Scheme For Heterogeneous Wireless Sensor Networks, Enaam A. Al-Hussain, Ghaida A. Al-Suhail

Karbala International Journal of Modern Science

Heterogeneous Wireless Sensor Networks (WSNs) involve nodes with varying capabilities, such as different energy levels, sensing ranges, and computational abilities, which enable them to execute different tasks professionally. Clustering techniques play a crucial role in improving energy efficiency and reliability in WSNs. The evolution of cluster based WSNs from homogeneous into heterogeneous techniques allowed the deployment of smart devices capable of performing complex operations in in diverse environments. However, the heterogeneity of nodes necessitates more sophisticated and adaptive algorithms to fully exploit these capabilities. This paper proposes a new protocol, referred to as IT2F-HLEACH, which integrates Interval Type-2 Fuzzy Logic …

Comprehensive Review On The Application Of Bio-Immunoinformatics In The Development Of Highly Ef-Fective New Candidate Vaccines Against Tuberculosis, Ahyar Ahmad, Andriansjah Rukmana, Miski A. Khairinisa, Dian A. E. Pitaloka, Rosana Agus, Rusdina B. Ladju, Tarwadi Ahmad, Astutiati Nurhasanah, Carina C. D. Joe, Muhammad N. Massi, Harningsih Karim, Irda Handayani, Siti Roszilawati Binti Ramli

Karbala International Journal of Modern Science

Tuberculosis (TB) remains a significant public health challenge worldwide. Currently, Bacillus Calmette-Guerin (BCG) is the only vaccine available for TB prophylaxis. However, the efficacy of the BCG vaccine against adult pulmonary TB is considered inconsistent. This condition encourages researchers to look for more effective options, such as subunit vaccines. This condition requires the development of a more effective subunit vaccine to protect active TB in productive and adult ages. There is an urgent need for more effective vaccines, as the Bacillus Calmette-Guérin (BCG) vaccine currently available has inconsistent efficacy and is only partially effective in adults. Bio-immunoinformatics, an interdisciplinary field …

Transcriptional Profiles Reveal Physiological Mechanisms For Compensation During A Simulated Marine Heatwave In Yellowtail Kingfish (Seriola Lalandi), Sharon E. Hook, Ryan J. Farr, Jenny Su, Alistair J. Hobday, Catherine Wingate, Lindsey Woolley, Luke Pilmer

Fisheries Research Articles

Background

Changing ocean temperatures are already causing declines in populations of marine organisms. Predicting the capacity of organisms to adjust to the pressures posed by climate change is a topic of much current research effort, particularly for species we farm or harvest. To explore one measure of phenotypic plasticity, the physiological compensations in response to heat stress as might be experienced in a marine heatwave, we exposed Yellowtail Kingfish (Seriola lalandi) to sublethal heat stress, and used the transcriptome in gill and muscle, benchmarked against heat shock proteins and oxidative stress indicators, to characterise the acute heat stress …

Leveraging Large Language Models For Knowledge-Free Weak Supervision In Clinical Natural Language Processing, Enshuo Hsu, Kirk Roberts

Faculty, Staff and Student Publications

The performance of deep learning-based natural language processing systems is based on large amounts of labeled training data which, in the clinical domain, are not easily available or affordable. Weak supervision and in-context learning offer partial solutions to this issue, particularly using large language models (LLMs), but their performance still trails traditional supervised methods with moderate amounts of gold-standard data. In particular, inferencing with LLMs is computationally heavy. We propose an approach leveraging fine-tuning LLMs and weak supervision with virtually no domain knowledge that still achieves consistently dominant performance. Using a prompt-based approach, the LLM is used to generate weakly-labeled …

Meeting Carbon Dioxide Removal Demand In 2030: The Potential Of Macroalgae Cultivation And Harvest, Lotta Siebert, Jiajun Wu, Lena-Katharina Bednarz, David Keller, Felix Meier, Christine Merk, Sonja Peterson, Wilfried Rickels

Journal of Ocean and Coastal Economics

A growing number of countries have announced net-zero and net-negative emissions targets, but only a few countries provide incentives for carbon dioxide removal (CDR). We derive estimates of countries' hypothetical demand for CDR in 2030 based on their emissions reduction targets under the Paris Agreement. The aggregated average global demand for CDR in the compliance year 2030 is 1064 MtCO₂, 353 MtCO₂, and 124 MtCO₂ for the low, medium, and high-cost CDR scenarios, respectively. This demand comes exclusively from countries and regions with relatively high GDP per capita, relatively high abatement costs and a limited …

Unraveling Genetic Links Between Diabetes And Heart Failure-A Machine Learning Approach, Sunakhi Sahoo, Marzieh Ayati

Research Symposium

Background: Diabetic heart failure (DHF) is defined as a chronic and progressive disease which is associated with both diabetes and heart failure (HF). Even though there have been many developments in the knowledge of these diseases, there is still much to learn about the genetic crossovers between the two. In this study, we identified genes that are associated with diabetic heart failure and heart failure by using gene expression data from patients with DHF, HF, and a control group of patients who died of natural causes. We sought to identify genes that had altered expression levels which could possibly play …

Earth Orbital Rhythms Links Timing Of Deccan Trap Volcanism Phases And Global Climate Change, Thomas Westerhold, Edoardo Dallanave, Donald Penman, Blair Schoene, Ursula Röhl, Nikolaus Gussone, Junichiro Kuroda

Geosciences Faculty Publications

At the end of the Cretaceous, the massive Deccan trap (DT) volcanic eruptions are regarded as the primary driver of global climate deterioration. Accurate age models are key to unravel the sequence of events related to DT volcanism onset and effects on the global climate system. We establish a direct geochemical link between DT volcanism as recorded in marine osmium isotopic data and global climate change documented in benthic foraminifera carbon and oxygen isotope records. Based on our state-of-the-art astronomically calibrated age model, two major shifts in marine ¹⁸⁷Os/¹⁸⁸Os at 66.49 and 66.28 million years ago are …

Biochemical, Structural, And Conformational Characterization Of A Fungal Ethylene-Forming Enzyme, Shramana Chatterjee, Joel A. Rankin, Mark A. Farrugia, Simahudeen Bathir J S Rifayee, Christo Z. Christov, Jian Hu, Et. Al.

Michigan Tech Publications

The ethylene-forming enzyme (EFE) from the fungus Penicillium digitatum strain Pd1 was heterologously produced in Escherichia coli and its properties were compared to the extensively characterized bacterial enzyme from Pseudomonas savastanoi strain PK2. Both enzymes catalyze four reactions: the conversion of 2-oxoglutarate (2OG) to ethylene and CO₂, oxidative decarboxylation of 2OG coupled to _L-arginine (_L-Arg) hydroxylation, uncoupled oxidative decarboxylation of 2OG, and the production of 3-hydroxypropionate (3-HP) from 2OG. The strain Pd1 enzyme exhibited a greater ratio of ethylene production over _L-Arg hydroxylation than the PK2 strain EFE. The uncoupled decarboxylation of 2OG and …

The Reaction Of Eragrostis Curvula To N And K Fertilization, N.F G. Rethman, C C. De Witt

IGC Proceedings (1977-2023)

Fertilization programmes for grass pastures in Southern Africa have placed emphasis on N and Pin the past. With the depletion of soil K, however, K fertilization is becoming increasingly important. Reliable response data for N and K fertilization is needed optimize fertilization level.

Efficacute De La Fertilization Minerale Des Prairies En Sol Alluvial, L Solimowski, R Lyszczarz, R Dembek

IGC Proceedings (1977-2023)

Le sol alluvial de la vallee basse de la Vistule offre des lieux naturels favorables a la culture des herbages (Cieslinski et Raszeja, 1983). On pratique assez souvent la seule fertilisation azotee, consideree comme la plus rentable. L'objectif de notre essai etait d'etudier !'influence des differents elements fertili­sants apportes pendant plusieurs annees sur la culture, !'evolu­tion de la composition botanique de la prairie, de la teneur en elements nutritifs de l'herbe et de la fertilite du sol.

Effects Of Fertilization And Liming On The Improvement Of Native Pastures, Maria E.V Lourenco, R.J. M. De Carvalho, Maria De L.A.P Da Silva

IGC Proceedings (1977-2023)

Native pastures in Portugal have relatively low productivity. The yield and botanical composition of such grasslands, where some legumes species are present, can be improved by fertilization with phosphorus application and/or also liming (Carter, 1983). The presence of legumes in pasture can also be improved by molybdenum application (Lattan et al., 1984). Significant increases of phosphorus concentration in plants (Garden et al., 1978) and on soil available phosphorus (Gregan et al., 1980) due to phosphorus application have also been reported.

The objectives of this study were to evaluate the potential of such treatments on the improvement of native pastures.

Influence Of Npk Fertilizer On Chrysopogon-Agrostis Swards, T Totev, V Vulkov

IGC Proceedings (1977-2023)

The Chrysopogon gryllus-Agrostis capillaris transitional association is commonly found in Bulgaria natural meadows. The aim of our investigation was, by applying different rates and proportions, to determine NPK fertilizer influence on the productivity, botanical composition and crude protein yield of the sward.

Derivation And Testing Of Nutrient Norms For White Clover, Marta J. Rodreiguez

IGC Proceedings (1977-2023)

Foliar analysis has been practiced widely to diagnose the nutrient status of crops in order to give fertilizer recommenda­tions. The changing nature of plant tissue mineral concentra­tions imposes limitations on the use of foliar analysis for diag­nostic purposes. In this way, sampling of particular plant parts at particular stages of development is required to assess the nutrient status of crop and forage plants. The Diagnosis and Recommendations Integrated System (DRIS) was developed by Beaufils (1973), based on a holistic experimental approach, as a means of coping with the problems derived of the dynamic nature of foliar composition. Three concepts are …

Influence De La Fertilization Sur La Repartition De La Biomasse Vegetale En Prairie Temporaire, R Lyszczarz

IGC Proceedings (1977-2023)

L'influence de la fertilisation minerale et organique sur le rendement et la composition botanique de la partie recoltable de la prairie est bien connue. Cependant nous n'en savons pas autant en ce qui concerne !'action de cette fertilisation sur Jes autres parties de la biomasse vegetale. Une etude approfondie de sa productivite, structure, composition chimique et reparti­tion permettrait d'evaluer son importance dans l'ecosysteme agricole (Hurto et al., 1980; Lutke, 1983).

Liming And Phosphate Fertilization On Forage Production, Mineral Composition And Nodulation On Pigeonpea (Cajanus Cajan (L.) Millsp.), V T. Paulino, N L. Costa, C.G S. Pedreira

IGC Proceedings (1977-2023)

For legume forage plant contribute effectively to grassland improvement, mainly through high herbage yields and fixation of atmospheric N, it is necessary to have a better knowledge of its nutritional requirements, since pigeonpea does not grow well on acidic soils with high levels of Al and poor in Ca, Mg and P. Ogunwale and Olaniyi (1978) reported a significant increase on forage production and P and Ca uptake for pigeonpea as a result of phosphate fertilization, while Abrufi.a et al., (1984) observed that lime improved growth, nodulation and P and Ca uptake, but with little effect on crude protein con­tent. …

Effect Of Rate And Placement Of Phosphorus On The Establishment Of Legume-Based Tropical Pastures On Oxisols, J M. Spain, R Gualdron, C Perdomo, P Avila

IGC Proceedings (1977-2023)

The phosphorus (P) requirements of perennial forage plants adapted to tropical savanna ecosystems are low compared to those of most annual crops or unadapted forage species (Andrew and Jones, 1985; CIAT, 1978). Perrenials are capable of accumulating P in the biomass over time and good pasture management favors micro-environmental conditions which can result in the recycling of P almost independent of the mineral soil (Spain and Salinas, 1985). It has been shown for crops that for maximum fertilizer efficiency at low rates, P should be concentrated near the seed to optimize fertility for seedlings, but there are few data for …

Use Of Cattle Slurry For Irrigated Temporary Grasslands, J Tomasik

IGC Proceedings (1977-2023)

Available literary sources deal almost exclusively with the slurry application for semi-natural grassland in diluted form, with varying rations of slurry and water (Rais and Kralovec, 1977, 1978 ; Schechtner, 1978 ; Schechtner, 1980; Sharda et al.,

1977 ; Tuney, 1980), The efficiency, i.e. production effect of slurry compared with industrial fertilizers varies in the quoted cases over the range of 50 to 111 %, depending on the most diversified natural and operating factors. We have therefore undertaken the task of identifying potential production capaci­ties of the selected set of monocultures of grasses in the appli­cation of increasing dosages of …

Influence Of Phosphorus Fertilization On Bahiagrass And Water Quality, G G. Payne, J E. Rechcigl

IGC Proceedings (1977-2023)

Increasing evidence suggests that P concentration is the pri­mary factor controlling the eutrophication of many surface waters (Taylor and Kilmer, 1980). Leaching and runoff losses of soluble P fertilizers applied to beef cattle pastures to increase forage production are believed to be a significant source of this P entering the water. Despite the fact that nearly one million hectares of bahiagrass are grown annually in Florida, relatively little is known about the nutrient requirements of bahiagrass for optimum forage production and quality. Currently, fertil­izer rates of up to 112 kg P2Os/ha are being recommended for bahiagrass pastures. There is mounting …

Potassium And Sulfur Fertilization Effects On Alfalfa Yields And Concentrations Of Minerals And Amino Acids, M Collins

IGC Proceedings (1977-2023)

Alfalfa (Medicago sativa L.) is an important perennial legume in ruminant feeding programs. Mechanical dewatering of alfalfa herbage yields a protein concentrate that contains 8.5 % of the DM and 29 % of the N from the original crop (Collins, 1985). Nonruminants would benefit from an increase in S amino acid concentrations of alfalfa since S amino acids limit utilization of leaf proteins (Byers, 1983). A greenhouse study was conducted to determine the effects ofK and S fertiliz­ation on the yield, composition and amino acid concentrations of alfalfa herbage.

Effects Of Phosphogypsum, Phsophorus And Lime On Andropogon Gayanus Kunth, V T. Paulino, E Malavolta

IGC Proceedings (1977-2023)

There are several factors which determine the low animal productivity in the tropics. Among these factors, inadequate nutrition is the most limiting one followed by the low natural fertility and high acidity of the soils in these regions. Previous findings have shown important soil nutrient deficiencies in some Brazilian cerrado soils. Malavolta (1952) demonstrated sulfur (S) deficiencies, while Werner et al. (1967) found plant response to S. Responses to phosphorus addition are very common in this kind of soil and in tropical America there are rates of phosphorus being applied, which sometimes are uneconomical for the formation and maintenance of …

Red Clover Needs For Mobybdenum And Boron Soil Threshold Values Of Deficiency And Toxicity, H Gembrazewski

IGC Proceedings (1977-2023)

Red clover is a precious and promising fodder crop in Poland. Even on acid soils its yields are satisfying, if sufficient level of exchangeable Ca is present. Symbiotically fixed N covers N needs, but fixing effectiveness and rate of further changing of nitrogen compounds depends on optimal molybdenum supply. It is a component of such enzymes as nitrogenase and nitrate reductase, which make it necessary for Rhizobium as well as clover. However, Mo surplus is toxic for animals (the relation of Mo:Cu is of import­ance). As other leguminous plants, red clover needs much boron. Yield of 15 t of dry …

An Effective P Application Method For Dactylis Glomerata, M Hojito, A Nishimune, M Noshiro

IGC Proceedings (1977-2023)

There are two practical ways to apply phosphorus (P) fertil­izer on cutting type grasslands in Hokkaido, Japan. The first one is to apply P at a rate of about I 00 kg P2Os/ha in the spring (April) in one application. Some reasons for this method have been explained (Hayakawa et al., 1951). However, in some cases, it is more convenient for practical management to apply three equal amounts of P, at three different times during the year (April, June and August) the same as other fertilizers such as nitrogen or potassium. That is a second way. Grassland soil is acidified …

Distribution Of Phosphorus And Aluminum In Acidic Pasture Soils And Effects On Growth Of Subterranean Clover, M J. Mclaughlin, T R. James

IGC Proceedings (1977-2023)

In south-eastern Australia, pasture improvement and the use of superphosphate fertilizers have resulted in the gradual acidi­fication of many soils under subterranean clover pastures (Wil­liams, 1980). Despite high values of exchangeable and extract­able aluminium (and low pH) recorded in many soils, there are few reports of significant responses of clover growth to lime application in the field. In this study we examined the vertical distribution of CaCb-extractable aluminium (Al) and available phosphorus (Bray P) in pasture topsoils. We also simulated the conditions in the field sites by investigating in solution culture the response of subterranean clover to various concentrations of …

Relations Entre Facteur Hydrique, Drainage Er Evolution De La Flore Des Prairies En Lorraine, S Oberle, S Plantureux, R Bonischot, A Guckert

IGC Proceedings (1977-2023)

Une part non negligeable (plus de 50 %) des Surfaces Agri­coles lorraines est occupee par des prairies permanentes. Or, nombre de ces dernieres souffrent d'exces d'eau qui limitent gravement leur productivite. Le developpement recent d'opera­tion de drainage offre la possibilite de lever un facteur limitant et, ce faisant, d'augmenter significativement la qualite et la quantite du fourrage produit. Il convient toutefois de rappeler que Jes prairies permanentes sont constituees d'un ensemble d'especes vegetales en equilibre avec leur milieu ecologique. la perturbation de ce dernier, en !'occurrence la modification du regime hydrique, va entrainer la mise en place progressive d'un nouvel …

Influence Of Sulfur And Nitrogen On Bahiagrass, J E. Rechcigl, G G. Payne, R J. Stephenson

IGC Proceedings (1977-2023)

One of the most important forage crops grown in Florida is bahiagrass, with nearly 1 million ha grown annually. Bahia­grass is found on more acres than all other improved grass pastures combined. Average annual dry matter yields for bahiagrass range from 9 - 16 t/ha. Bahiagrass is adapted to most climatic conditions throughout the state and can be grown on upland well drained sands as well as poorly drained « flatwoods » soils. Until recently, little attention has been focused on the need for S fertilization for plant growth. This is understandable since, in the past fertilizers were contaminated with …

The Depletion Of Soil K As Affected By Fertilization Of Eragrostis Curvula Used For Hay Production, N.F G. Rethman, C C. De Witt

IGC Proceedings (1977-2023)

Eragrostis curvula is widely planted as a hay crop in the summer rainfall areas of South Africa. For many years the main yield determinants have been regarded as N and P. As a result of the depleting effect of crop removal low K status is becoming more common. The beneficial effect of K fertiliz­ation, in combination with N, has been illustrated by Miles et al. (1986) and Rethman and De Witt (1839). Information on the rate of build up and/or depletion of soil K under different fertilization regimes is, however, required to facilitate the plan­ning of fertilization strategies. The monitoring …