Digital Commons Network^™

Valorisation Des Paturages De Haute-Belgique Par La Vache Laitiere A Travers Deux Systemes D'Exploitation Differents, P Limbourg, M Gielen, L Istasse, J M. Bienfait

IGC Proceedings (1977-2023)

La prairie permanente occupe en Haute-Belgique environ 70 % de la surface agricole: il importe done d'en exploiter tou­tes les potentialites. Si le chargement constitue le facteur essen­tiel de la bonne utilisation de l'herbe au paturage, les modalites d'exploitation jouent egalement un role non negligeable. C'est ainsi que le paturage continu intensif est tres attractif aupres des eleveurs en raison de l'economie de main-d'ceuvre qui en resulte. Des experiences anterieures ont cependant demontre la plus faible productivite en herbe d'un tel systeme par rapport au paturage classique en rotation, du fait notamment de la reduction des temps de repos. Dans …

New Grazing Animal Production System In Japan, H Kobayashi, K Ochiai, S Shioya, Y Togamura, T Ami, N Harashima, K Sato, M Nashiki

IGC Proceedings (1977-2023)

The amount of animal production from pasture by conven­tional grazing system in Japan stays at low level as 300 kg body weight gain of steers per hectare in one grazing period. This is supposedly caused by the following problems concerning graz­ing in Japan ; cold or hot temperature, rainy season, steep slope pasture, low fertilizer application, sharp seasonal fluctuation of grass production (spring flush, summer depression), faulty warm-up grazing, lots of blood-sucking insects and diseases (piroplasmosis, pneumonia etc.). Settling these problems, we have developed a new short rot­ational grazing system to get three times animal production (1,000 kg body weight …

Determination Of The Number Of Paddocks Necessary For The Rotation Of Grazing Dairy Cows, A Senra, J Ugarte, M A. Menchaca, J Galindo

IGC Proceedings (1977-2023)

Great importance in the grassland management has been given to the paddock system, since it allows animal rotation. However, fencing costs must be minimal without affecting ani­mal or pasture performance. Thus, it is necessary to obtain results that can be applied to a wider range of grasslands, tak­ing into consideration that each pasture could show a different performance. The objective of this study was aimed to deter­mine the minimum number of paddocks necessary for grazing dairy cows with pastures of greater and lesser aggressiveness.

Etude Comparative De Deux Varietes De Fetuque Elevee De Qualite Differente Conduites En Paturage Continu Avec Vaches Laitieres, J C. Emile, M Gillet, L Huguet

IGC Proceedings (1977-2023)

Dans des essais anterieurs (Gillet et al., 1986 et 1989), nous avons mis en evidence chez la fetuque elevee Festuca arundin­acea Schreb. des differences varietales pour la valeur alimen­taire avec des moutons a l'entretien, des chevres laitieres et des vaches laitieres. Ces differences etaient observees a l'auge. Nous avons voulu verifier si, et dans quelles conditions, elles se retrouvaient !ors d'une utilisation en paturage continu par des vaches laitieres. (cf Roden et al., 1987). Notre demarche peut aider a mettre en evidence les conditions de valorisation de la qualite dans les especes fourrageres.

Phosphorus Fertilizer And The Long Term Productivity Of Nitrogen Fertilized Dairy Pasture, T M. Davison, W N. Orr, B A. Silver, F Duncalfe

IGC Proceedings (1977-2023)

Milk production responses to nitrogen fertiliser on tropical grass systems have been well documented (Davison et al. 1985a; 1985b). However, there are no reports on the long term phosphorus fertiliser requirements of dairy pastures. This experiment, which ran for 7 years, was designed to determine the long term phosphorus fertiliser requirements, for mainten­ance of a grass pasture fertilised with nitrogen. The effects of fertiliser level were monitored in terms of changes in soil fer­tility, plant productivity and milk yield.

Prospects For Legume-Based Dairying In Cool Temperate Conditions, M Ryan

IGC Proceedings (1977-2023)

There is increasing interest in exploiting legume-based pastures in the temperate zones of NW Europe-their value for lamb and beef production being recently documented (Stewart, 1984; Davies and Munro, 1988). Legume-based intensive dairying is currently not prevalent north of - 49 NL but quota restrictions leading to fewer cows may create conditions favourable to legumes within dairying. This paper describes the results of experimentation comparing N and legume-based dairying in cool temperate conditions and assesses future pros­pects.

Simplification De La Distribution Des Aliments Concentres Aux Vaches Laitieres Pendant La Periode De Paturage, J I. Andries, D L. De Brabander, Ch V. Boucque, F X. Buysse

IGC Proceedings (1977-2023)

En vue de remplacer le systeme complique de distribution de concentres selon le niveau de production, la recherche s'efforce, ces dernieres annees, de simplifier la distribution de concentres pour le betail laitier. On a examine si une distribution d'une quantite egale de concentres a toutes Jes vaches d'un groupe en fonction d'un niveau de production laitiere preetabli, peut etre un systeme valable pour la periode de paturage. Ce systeme est voisin de la « distribution d'une quantite fixe de concentres », aussi appele "flat rate feeding", dont la convenance pour la periode de sta­bulation a deja ete demontree plusieurs fois …

Production And Utilization Of High-Quality Cold-Hardy Forages, D W. Koch, C Kercher, D Agee, R Jones

IGC Proceedings (1977-2023)

Grazing is generally the least expensive method of forage utilization ; however, in temperate climates the growing season is relatively short and perennial forages are at peak nutritive value for a relatively short time. Brassica spp. have several characteristics which make them prime candidates for extending the grazing season : (1) they mature rapidly (Guillard and Allinson, 1984); (2) they are frost tolerant and maintain high nutrient value well during cold weather (Jung et al., 1984); and (3) they can be established with minimum tillage techniques (Jung et al., 1984; Koch et al., 1987). The purpose of this study was …

Multispecies Grazing Systems In Iowa, Midwestern Usa, W F. Wedin, M A. Sanderson, C Ohlsonn, D G. Morrical

IGC Proceedings (1977-2023)

Combining species of grazing animals such as cattle and sheep into appropriate grazing systems may help producers to use grazing land resources more efficiently (Heinemann, 1970 ;Van Keuren and Parker, 1967: Winrock, 1985). Earlier pasture research on lands unsuited for row-crop production has shown that renovation to incorporate a pasture legume has been very beneficial (Wedin et al., 1967). Yet, grazing manage­ment has lagged behind legume incorporation and fertilization (Wedin, 1985). Practices used in animal management must be taken into account in new grazing systems. In midwestern USA, producers commonly buy stocker steers in spring to take advantage of ample, …

Implications Of Grazing Experiments Which Employ Only One Grazing Level Per Treatment, David I. Bransby

IGC Proceedings (1977-2023)

Stocking rate is one of the most powerful driving variables in grazing ecosystems, but its influence on animal production is mediated through effects on pasture sward state, and associated effects on quantity and quality of forage consumed by animals. Consequently, confounding important sward state char­acteristics with treatments in grazing experiments will often mask treatment effects and lead to erroneous conclusions. While this problem has generally been recognized (e.g. Mott and Lucas, 1952; Wheeler et al. 1973 ; Hodgson, 1985), attempts to overcome it appear to be less than satisfactory. The aim of this paper is to discuss methods of stocking …

Experiments And Extension Of The Utilization And Improvement Of Sandy Rangelands In Nei Monggol (Inner Mongolia) Autonomous Region, China, Hou Yong-Zhang, Bai Ri-Sheng, Li Xhang-Chun

IGC Proceedings (1977-2023)

There are about 60 million ha. of rangelands available for livestock in Nei Monggol (Inner Mongolia) Autonomous Region. In the great vast area of rangelands there are 4 rather large sandy rangelands: 1) the Hulun Beir Sandy Rangeland; 2) the Kerching Sandy Rangeland ; 3) the Hunshandak Sandy Rangeland ; and 4) the Maowoos Sandy Rangeland. Their total area covers about 20.8 million ha and accounts for more than 1/3 of that available in N. M. A. R.

The Hulun Beir Sandy Rangeland located in the northeast part of N. M.A. R. - N 47° 40'-50°12' E 118°3!'-121°03' 355 km …

Production De Viande Bovine Et Ovine Sur Prairie Dans Les Monts Sudetes, Janina Fatyga

IGC Proceedings (1977-2023)

Par suite du depeuplement des villages et de !'abandon de la culture dans des zones situees en altitude dans !es Sudetes, on y observe l'accroissement de la superficie des paturages perma­nents. Des 1968, on y cree des zones de pature OU en ete de jeu­nes bovins et des ovins provenant des fermes situees plus bas sont conduits en permanence. L'importance du phenomene est illustre par le fait qu'a present, sur le terrain d'une seule region de Walbrzych, il y a 103 bases de pature d'une superficie de 7.000 ha, appartenant a 54 fermes d'etat ou paissent environ 25.000 ovins et …

A Standard Livestock Unit (Slu) For Defining Stocking Rate In Grazing Studies, D J. Minson, P C. Whiteman

IGC Proceedings (1977-2023)

The weight of animals and hence their intakes vary between grazing experiments and at different stages of the same study but the results are often presented as though these differences do not affect grazing pressure (Roberts, 1980). Thus we have sheep/ha and cattle/ha, with no indication of the effects that differences in intake and hence grazing pressure might have on the pasture/animal interaction. Pastures are utilized by different classes of stock and there is a need to have a common basis for comparing their productivity. These problems would be over­come by the use of a Standard Livestock Unit (SLU).

Combining Submicron Spectroscopy Techniques (Afm-Ir And O-Ptir) To Detect And Quantify Nanoplastics In Snow From A Utah Ski Resort, Sara L. Belontz, Janice Brahney, Caroline E. Caplan, Eoghan Dillon, Ting Yan, Gerardo Dominguez

Watershed Sciences Faculty Publications

Atomic force microscopy-based infrared spectroscopy (AFM-IR) and optical photothermal infrared (O-PTIR) spectroscopy are cutting-edge techniques used for precise nanoscale chemical analysis, capable of detecting and characterizing particles smaller than 1 μm. In this study, we applied both techniques to analyze snow subsamples collected from Beaver Mountain, Utah. Quantification by AFM-IR identified a concentration of 1.50 × 10^–1 μg/mL of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), a copolyester known for its biodegradability and biocompatibility and that was the only polymer detected. Notably, 96% of particles had thicknesses below 1 μm, with the smallest particle recorded at 14 nm in height, demonstrating the advantage …

Efficient Methods And Algorithms For Analyzing Stochastic Systems, Mohammad Ahmadi

USF Tampa Graduate Theses and Dissertations

This dissertation addresses the challenges of stochastic analysis of safety-critical systems with biological components, where unexpected behavior can lead to catastrophic events. Two fundamental challenges hinder the analysis of such systems: their typically large or infinite state spaces, and the extreme rarity of error states of interest. While Monte Carlo simulation can analyze biochemical systems without storing the state space, accurately estimating rare event probabilities becomes computationally prohibitive. Conversely, probabilistic model checking excels at analyzing extremely low probability events but becomes impractical for systems with large or infinite state spaces due to memory constraints.This work proposes two main contributions to …

Isotopic Fingerprints Of The Coastal Margins: Tracing Dissolved Iron Sources From Land To Sea, Hannah R. Hunt

USF Tampa Graduate Theses and Dissertations

Dissolved iron (dFe) is an essential micronutrient for sustaining life in the surface ocean. Nearly four decades of research dedicated to understanding sources, cycling and biogeochemical modifications of Fe sources in the global oceans has generated a significant body of research supporting the idea that the concentration of Fe in the surface ocean exerts significant controls on primary production and carbon uptake in the surface ocean. Creation of the GEOTRACES program greatly increased the spatial and temporal resolution of high-quality trace element and trace element isotope data revealing new insights into the global Fe cycle, especially through the use of …

Enhancement Of Phenolic, Flavonoid, And Biological Activi-Ties In Fermented Pea (Pisum Sativum) Extracts Via Frac-Tionation, Anastasia Fitria Devi, Euis Filailla, Setyani Budiari, Hani Mulyani, Nina Artanti

Karbala International Journal of Modern Science

In this study, peas were fermented for 24 h using either Rhizopus oligosporus or Aspergillus oryzae. The resulting fermented peas, along with unfermented peas, were extracted using either methanol or water. The methanol extracts showed greater improvements following fermentation compared to the water extracts, as shown by the IC₅₀ values, which progressed from >500 to ≤200 µg/mL, as determined via the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method. Consequently, further fractionations focused on the methanol extracts of the fermented peas. Six bands were resolved on thin-layer chromatography plates for each extract. Their positions demonstrated significant effects (p < 0.05) on bioactivity. The bottom band (i.e., Band 1) exhibited approximately 70% inhibition, as determined via the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and 35% inhibition according to the ABTS method, while the other bands displayed lower to negligible inhibition. Band 5 displayed 85–97% cytotoxicity, while Band 1 did not show any cytotoxicity. The total phenolic content (TPC) of Band 1 and the total flavonoid content (TFC) of Band 6 increased 4.4- and 8.9-fold, respectively, compared to those of the unfractionated extracts. Furthermore, a correlation analysis revealed a strong correlation between TFC and the three observed bioactivities. The different cultures significantly (p < 0.05) affected the antioxidant activity of either the fermented pea extracts or the resulting bands, as determined via the DPPH method. These results indicated that fermentation enhanced the antioxidant activity of peas. Subsequent fractionations separated and concentrated compounds from the fermented peas, improving the bioactivities, TPC, and TFC of the resulting fractions.

Synthesis Of Gold Nanoparticles Using Hordeum Vulgare Leaf Extract And Their Antibacterial Activity, Mohamed M. Sehree, Shakir Ghazi Gergees, Pakhshan A. Hassan

Karbala International Journal of Modern Science

Plant extracts and gold nanoparticles are promising alternatives for combating antibiotic resistance in light of the increasing bacterial resistance. Leaf extract of barley was used to synthesize gold nanoparticles (AuNPs). Barley gold nanoparticles (BL-AuNPs) were produced by adjusting some reaction parameters. These BL-AuNPs were characterized through employing the UV-visible spectroscopy technique, the scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDX). BL-AuNPs were tested for antibacterial efficacy against two strains of Gram-negative bacteria, clinically isolated and considered as multidrug-resistant pathogens, Acinetobacter baumannii and Salmonella typhi. The antimicrobial efficiency of the compounds was evaluated …

The Impact Of Microplastics On Water Quality, Heavy Metals, And Health Risks In Bioflocbased Tilapia Farming Systems, Dian Rizky Afriani, Deswati Deswati, Rahmiana Zein, Putri Ramadhani

Karbala International Journal of Modern Science

Along with microplastics, pollution of heavy metals, including iron (Fe), zinc (Zn), and copper (Cu), in freshwater ecosystems poses a serious environmental threat that can adversely affect human health. This study investigates the use of biofloc technology to reduce microplastic and heavy metal contamination while improving water quality. By utilizing microbial aggregates that capture microplastic and heavy metal particles through flocculation and biosorption processes, four experimental treatments were applied, i.e.: A (without biofloc and microplastics); B (with biofloc, without microplastics); C (with biofloc and low-density polyethylene microplastics); and D (with biofloc and high-density polyethylene microplastics). The results indicate that fish …

Colloidal Hot-Injection Synthesis Of Cubse2 Nanocrystals: Tetragonal Chalcogenide Templates For Superionic Lithiation Pathways, Yunhao Xu, Niket Powar, Kelly Zervos, Isabella Campbell, Simran Choudhary, Layan Al-Khaled, Progna Banerjee

Chemistry: Faculty Publications and Other Works

Copper boron selenides represent a previously unexplored class of materials with tetragonal structural symmetries and bonding chemistries potentially relevant to lithium-ion conduction. In this work, the first colloidal synthesis of nanocrystalline copper boron selenides is reported, enabling access to nanocrystals with reaction times shown as the synthetic knob to control composition and morphology. Copper boron selenide nanocrystals are synthetically designed as precursors for lithiation reactions aimed at probing structural evolution and ionic mobility pathways toward superionic conductors applicable as solid electrolytes in lithium-ion batteries. Postsynthetic lithiation studies under ambient conditions reveal a series of phase transformations and morphological changes, which …

Exploring The Translation Lookaside Buffer (Tlb) For Low-Level Task Differentiation And Classification, Cristian Agredo, Daniel F. Koranek, Christine M. Schubert, Jose A. Gutierrez Del Arroyo, Tor J. Langehaug, Scott R. Graham

Faculty Publications

The primary focus of modern Central Processing Unit (CPU) technologies is performance improvement, with security often considered a secondary concern. As a result, vulnerabilities within the system are overlooked. While significant research, both offensive and defensive, has been conducted on CPU caches, relatively little attention has been given to the Translation Lookaside Buffer (TLB) due to its perceived lack of data granularity. Prior studies have typically combined multiple Hardware Performance Counters (HPCs) or relied on timing analysis to extract meaningful insights. In contrast, this study introduces a novel methodology that leverages only TLB related HPCs for multi-task classification, without incorporating …

Selecting An Internal Carbon Price For Academic Institutions, Alexander R. Barron, Dimitra Prassa

Environmental Science and Policy: Faculty Publications

Many businesses have adopted internal carbon prices (ICPs) to help drive smarter business decisions, innovation, and emissions reductions. The objective of this white paper is to provide a framework that institutions, particularly those in higher education, can use as they think about selecting a price in the context of their own goals for an ICP.

Second Nature’s recent 2025 Carbon Markets and Offsets guidance recommends that: “colleges and universities develop a climate action strategy that includes some type of internal carbon pricing, either as a mechanism to inform climate-positive decision-making or as a mechanism for generating revenue estimates for decarbonization …

Synthesis Of Carboxymethyl Cellulose From Nypa Fruticans For Coating Metformin On Zn-Based Metal-Organic Framework Nanohybrid As A Bionanocomposite Drug Delivery System, Delviani Delviani, Viola Giary Rizkillah Maharani, Putri Nur Shadrina, Tri Warni, Regina Wan Azizah, Indra Lasmana Tarigan, Madyawati Latief

Makara Journal of Science

Diabetes is a common and rapidly spreading disease that affects 6.6% of the world’s population. Metformin hydrochloride (HCl) is an effective oral pharmacological treatment for type 2 diabetes mellitus (T2DM) patients because of its capability to reduce glucose levels and the risk of hypoglycaemia. However, gastrointestinal sensitivities to this drug can cause diarrhoea, nausea, vomiting and stomach ache. An alternative is controlled release technology by coating metformin with carboxymethyl cellulose (CMC) and zinc-metal-organic framework (Zn-MOF) through the encapsulation method. Therefore, this study aimed to coat metformin formulations using CMC in a Zn-MOF to control release into the body and minimise …

Removal Of Heavy Metals From Motorcycle Washing Wastewater Using A Zeolite-Embedded Sheet, Noverita Dian Takarina, Hanies Ambarsari, Sekar Arum Pramudyawardhani, Maria Fatima Niken Kris Rahmawati, Erni Johan, Naoto Matsue, Mohammad Afdhal Adidharma

Makara Journal of Science

The increased use of motorcycles for transportation has heightened environmental challenges related to their washing, particularly the discharge of untreated wastewater containing heavy metals, such as copper (Cu) and lead (Pb), into drainage systems. This study explored the effectiveness of zeolite-embedded sheets (ZESs) in the removal of these pollutants from motorcycle washing wastewater. ZESs were prepared through treatment of nonwoven sheets with Linder Type A (LTA Zeolite) powder and heat treatment, washing, and drying. Characterization was performed via scanning electron microscopy with energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. Over a 60 min adsorption period, the ZES achieved removal …

Kolmogorov–Smirnov-Based Edge Centrality Measure For Metric Graphs, Christina Durón, H. Kravitz, Moysey Brio

Mathematics and Statistics Faculty Publications and Presentations

In this work, we introduce an edge centrality measure for the Helmholtz equation on metric graphs, a particular flow network, based on spectral edge energy density. This measure identifies influential edges whose removal significantly changes the energy flow on the network, as indicated by statistically significant p-values from the two-sample Kolmogorov–Smirnov test comparing edge energy densities in the original network to those with a single edge removed. We compare the proposed measure with eight vertex centrality measures applied to a line graph representation of each metric graph, as well as with two edge centrality measures applied directly to each …

Removal Of Heavy Metal Iron(Ii) Ions From Wastewater Using An Ultrasonic System With Climbazole-Alcohol, Melek Gökmen Karakaya, Bahdi̇şen Gezer, Abdullah Menzek, Özlem Gündoğdu Aytaç

Turkish Journal of Chemistry

Climbazole (CBZ) is an antifungal active pharmaceutical ingredient often used in antidandruff products. In this study, the ketone group in the racemic CBZ molecule was reduced to synthesize CBZ-alcohol, named 1-(4-chlorophenoxy)-1-(1H-imidazol-1-yl)-3,3-dimethylbutan-2-ol, and the optimum adsorption conditions were investigated for removing iron(II) (Fe2+) ions from wastewater by adsorption from aqueous solutions using the economical and environmentally friendly ultrasonic method. The parameters and levels used in the study were designed using response surface methodology and model equations were derived to optimize the results. The independent variables selected were the initial pH (1, 3, and 5), adsorption time (30, 45, and 60 min), …

Enhanced And Proficient Chitosan Membranes Embedded With Polyaniline-Tio2 Core-Shell Nanocomposites For Fuel-Cell Hydrogen Storage, Mallikarjunagouda Patil, Sridhar N. Mathad, Arun Y. Patil, Abdulaziz Abdulah Al-Kheraif, Sachin Naik, Sajith Vellapally

Turkish Journal of Chemistry

This study investigates the preparation and properties of aniline polymerized in situ onto a nanosized TiO2 surface to form core-shell nanoparticles at ambient temperatures. The in situ polymerization of aniline to polyaniline (PANI), in conjunction with the utilization of an anionic surfactant, was employed in this investigation. The prepared PANI-TiO2 core-shell nanoparticles were integrated with chitosan at a gravimetric ratio and cast as core-shell nanocomposite membranes. The nanocomposites were subjected to structural analysis using Fourier transform infrared spectroscopy and X-ray diffraction patterns. The surface morphologies of the PANI and its nanocomposites were analyzed using scanning electron microscopy. Direct current conductivity …

1,3,4-Thiadiazole Derivatives As Pi3ks Inhibitor: Design, In Silico Studies, Synthesis, Characterization, And Antimicrobial Evaluation, Dharmvir Singh, Pankaj Kumar, Anoop Kumar, Vivek V. Bhosale, Kalicharan Sharma, Deepak Kumar, Ramchander Khatri, Tanuj Hooda, Amit Lather

Turkish Journal of Chemistry

Since PI3Ks are targeted by a variety of bacterial pathogens, they represent a promising target for host-directed immune therapy and may be beneficial in managing persistent bacterial infections. In the present study, computational studies of 5-(pyridin-4-yl)-1,3,4-thiadiazol-2-amine derivatives for phosphoinositide-3-kinases (PI3Ks) inhibitors were carried out using dock scores, Glide scores, and the MMGBSA dG method, with comparison to standard drugs (ofloxacin and fluconazole). A series of 5-(pyridin-4-yl)-1,3,4-thiadiazol-2-amine derivatives (D1-D17) were synthesized and evaluated for their in vitro antimicrobial activity against both gram-positive and gram-negative bacterial strains, as well as fungal strains, using the tube dilution method. The synthesized compounds were characterized …

Highly Sensitive And Selective Detection Of Klk4 In Human Serum With A Low-Cost Electrochemical Biosensor, Eli̇f Burcu Aydin

Turkish Journal of Chemistry

An ultrasensitive impedimetric immunosensor was fabricated using a poly(glycidyl methacrylate) (PGMA) polymer-covered indium tin oxide (ITO) platform for the quantification of kallikrein 4 (KLK4), an important prostate cancer biomarker. PGMA had suitable biocompatibility and nontoxicity for loading of antiKLK4 antibodies on the ITO substrate surface. Anti-KLK4 biomolecules were directly attached to the PGMA-covered electrode surface via epoxy groups of the PGMA polymer. The preparation method for the PGMA matrix-modified electrode was simple and inexpensive. The proposed biosensor immobilization layers coated on the ITO electrode were characterized with electrochemical techniques. The experimental parameters that affect biosensor response were optimized, and the …

Determination Of P53 Biomarker With A Smart Electrochemical Biosensor Based On Brush Polymer-Functionalized Disposable Electrode, Muhammet Aydin

Turkish Journal of Chemistry

A label-less impedimetric biosensor modified with poly(thiophene)-graft-poly(glycidyl methacrylate) polymer (PThi-g-PGM) was manufactured for p53 protein quantification in human serum samples. In this study, PThi-g-PGM polymer was synthesized, and this polymer matrix was coated using the spin-coating technique on the single-use indium tin oxide (ITO) substrate for anti-p53 antibody immobilization. The anti-p53 antibodies with high affinity for p53 proteins were covalently attached onto the PThi-g-PGM-coated electrode surface. In addition, the affinity of anti-p53 for the p53 protein was monitored at a constant frequency. Under optimized conditions, the impedimetric changes were linearly related to the p53 concentrations, ranging from 0.05 to 15 …