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Development, Isolation And Characterisation Of A New Non-Virulent Mimosine-Degrading Klebsiella Pneumoniae Strain From The Rumen Liquor Of German Steers By Using Ibt-Goettinger Bioreactor, Aung Aung, U. Ter Meulen, F. Gessler, H. Böhnel

IGC Proceedings (1977-2023)

The tropical legume Leucaena leucocephala (leucaena) has many uses, including: a potential source of firewood and timber; for soil erosion control (Dijkman 1950); to provide shade; to enhance soil fertility; and as a nutritious forage for animal feed (Ruskin 1977). It is widely used as forage for cattle in tropical agriculture (Shelton 1998). In Myanmar, leucaena is used as a protein source in urea-molasses multi-nutrient blocks for ruminants (Ni Ni Maw et al. 2004). However, the use of leucaena as ruminant feed is not without problems, because it contains mimosine, a toxic anti-nutritional factor limiting its use as animal …

Brs Paiaguás: A New Brachiaria (Urochloa) Cultivar For Tropical Pastures In Brazil, Cacilda B. Valle, Valéria P. B. Euclides, Denise B. Montagner, José R. Valério, Celso D. Fernandes, Manuel C. M. Macedo, Jaqueline R. Verzignassi, Luis A. Machado

IGC Proceedings (1977-2023)

Approximately half the world’s beef is produced in the tropics and subtropics, almost exclusively on pasture. While the world production of beef and veal increased 91% over the past 40 years, the increase in the tropics was 200%. Brazil has today the largest commercial cattle herd in the world (about 190 M head) and is the world's largest exporter of beef. The area of cultivated pasture increased from 30 to 100 Mha between 1970 and 1995 (IBGE 2006). This area has now stabilised or decreased despite the increase in beef production, which reflects the gain in area productivity. Part of …

Selecting New Brachiaria Humidicola Hybrids For Western Brazilian Amazon, Giselle M. L. De Assis, Cacilda B. Valle, Carlos M. S. De Andrade, Judson F. Valentim

IGC Proceedings (1977-2023)

Brachiaria humidicola is a perennial and stoloniferous forage grass, with excellent adaptation to infertile soils and waterlogging. Producers in northern Brazil have become interested in this species, especially after the degradation of large areas of lowly permeable soils sown with B. brizantha cv. Marandu, which is highly intolerant of soil waterlogging (Andrade and Valentim 2006). However, since only 3 cultivars of B. humidicola are registered in Brazil (Mapa 2013), there is a need to develop new cultivars adapted to the range of environmental conditions experienced. Recently, Embrapa Beef Cattle obtained intraspecific hybrids of B. humidicola that need to be evaluated …

Brs Mandobi: A New Forage Peanut Cultivar Propagated By Seeds For The Tropics, Giselle M. L. De Assis, Judson F. Valentim, Carlos M. S. De Andrade

IGC Proceedings (1977-2023)

Forage peanut (Arachis pintoi) is a forage legume of relatively recent use in beef and dairy cattle feeding. Although it is native exclusively to Brazil, the first cultivar, Amarillo, was released in Australia in 1987. Forage peanut has a great number of favourable attributes for establishing grass-legume mixtures, which guarantee the persistence and high yields of high quality forage in cultivated pastures. Therefore, studies with this species have intensified in many tropical regions of the world (Assis and Valentim 2009). In the state of Acre, western Brazilian Amazon, 137,000 ha are cultivated with forage peanut, cv. Belmonte, benefiting …

Direct And Indirect Effects Of Temperature And Prey Abundance On Bald Eagle Reproductive Dynamics, Joshua H. Schmidt, Judy Putera, Tammy L. Wilson

United States National Park Service: Publications

Understanding the mechanisms by which populations are regulated is critical for predicting the effects of large-scale perturbations. While discrete mortality events provide clear evidence of direct impacts, indirect pathways are more difficult to assess but may play important roles in population and ecosystem dynamics. Here, we use multi-state occupancy models to analyze a long-term dataset on nesting bald eagles in south-central Alaska with the goal of identifying both direct and indirect mechanisms influencing reproductive output in this apex predator. We found that the probabilities of both nest occupancy and success were higher in the portion of the study area where …

In‐Situ Extraction And Impregnation Of Black Walnut Husk Into Polyethylene Film Using Supercritical Carbon Dioxide With An Ethanol Modifier, Jonathan Wenzel, Veronica R. Moorman, Lihua Wang, Isaiah Spencer-Williams, Mitchell Hall, Cheryl Samaniego, Michelle Ammerman

Chemistry & Biochemistry Publications

Walnuts are commonly cultivated for their kernel, which is a rich source of antioxidant phenolic compounds. The husk likewise contains antioxidant and antimicrobial compounds, but is typically discarded without further processing. Antioxidant compounds are useful in creating active packaging films, but typically decompose at melt extrusion temperatures in polymer processing. Due to carbon dioxide's low critical point and ability to swell polymer films, supercritical carbon dioxide may be used to impregnate phenolic compounds into polymers. For this study, a novel technique is used to simultaneously produce walnut husk extracts and impregnate the extract into polymer films in the same batch …

Two Algorithms For The Reorganisation Of The Problem List By Organ System, Daniel B. Hier, Joshua Pearson

Chemistry Faculty Research & Creative Works

Objective Long Problem Lists Can Be Challenging to Use. Reorganization of the Problem List by Organ System is a Strategy for Making Long Problem Lists More Manageable. Methods in a Small-Town Primary Care Setting, We Examined 4950 Unique Problem Lists over 5 Years (24 033 Total Problems and 2170 Unique Problems) from Our Electronic Health Record. All Problems Were Mapped to the International Classification of Diseases, 10th Revision, Clinical Modification (ICD-10-CM) and SNOMED CT Codes. We Developed Two Different Algorithms for Reorganizing the Problem List by Organ System based on Either the ICD-10-CM or the SNOMED CT Code. Results the …

Landscape Scale: Inter- And Intraspecific Variation In Plant Interactions Along A Stress Gradient In The Sheep Range Of Nevada, Jordan Dowell

UNLV Theses, Dissertations, Professional Papers, and Capstones

Impending threats to shrubland ecosystems, posed by climate change, necessitate niche modeling efforts to project vegetation range shifts. However, efforts often remain unguided by individual-scale interspecific plant interactions. The stress gradient hypothesis posits that facilitation should increase in areas of high abiotic stress, only if the individuals are able to ameliorate the surrounding area via functional traits. The Sheep Range of Nevada was used to assess the role of functional traits as predictors of plant association. Larrea tridentata, Coleogyne ramosissima, and Artemisia nova were selected as shrubs with variable life history strategies and ranges in order to identify general patterns …

The Retrospective Analysis Of Radionuclides In Banded Coral From The Bikini Atoll, Athena Marie Gallardo

UNLV Theses, Dissertations, Professional Papers, and Capstones

Nuclear weapons testing conducted within the Bikini Atoll in the Marshall Islands resulted in local fallout contamination, including long lived isotopes of plutonium (Pu) and uranium (U), within the surrounding environment. The Bikini lagoon provides a continuous source of Pu (239+240Pu) and U (236U) to the Pacific Basin. The rate at which these isotopes are flushed from the lagoon to the ocean is balanced by inputs from remobilization of lagoon sediments. This research will provide a basis for predicting future change in the solubility and export potential of Pu and U in lagoon sediments. This is important to better understand …

Urinary Excretion Of Mimosine Derivatives By Cows With And Without Experience In Consumption Of Leucaena Leucocephala, N. E. Ruz-Ruiz, Eduardo G. Briceño-Poot, A. J. Ayala-Burgos, C. F. Aguilar-Pérez, Francisco J. Solorio-Sánchez, Luis Ramírez-Avilés, Juan C. Ku-Vera

IGC Proceedings (1977-2023)

Leucaena leucocephala is a leguminous tree widely distributed in the tropical regions of the world. In Mexico, it has been incorporated into silvopastoral systems and is highly regarded, owing to its high content of crude protein. Nonetheless, L. leucocephala contains secondary metab-olites, such as mimosine, a non-protein free amino acid, which may induce toxic effects in unadapted ruminants that consume the forage (Hammond 1995). Although Synergistes jonesii, an anaerobic bacterium, has the ability to degrade 3,4-DHP and 2,3-DHP to non-toxic compounds (Allison et al. 1992), in Mexico its presence has not yet been confirmed. Recent work has suggested …

Urinary Excretion Of Mimosine Metabolites By Hair Sheep Fed Foliage Of Leucaena Leucocephala, L. M. Contreras-Hernández, N. E. Ruz-Ruiz, Eduardo G. Briceño-Poot, Luis Ramírez-Avilés, A. J. Ayala-Burgos, C. F. Pérez-Aguilar, Francisco J. Solorio-Sánchez, Juan C. Ku-Vera

IGC Proceedings (1977-2023)

Leucaena leucocephala is an adapted legume widely distributed in the tropical regions of Mexico. The high crude protein content of leucaena leaves renders it appropriate for ruminant feeding under commercial conditions. However, the foliage contains the non-protein amino acid mimosine, which, if consumed in high amounts, may induce toxicity in animals which have not previously consumed the legume or without microorganisms capable of degrading mimosine and its derivatives 2,3-DHP (dihydroxypyridine) and 3,4-DHP (Hammond 1995, Palmer et al. 2010, Dalzell et al. 2012). Barros-Rodríguez et al. (2012) found that dry matter intake and weight gain were reduced when …

Herbage Accumulation, Nutritive Value And Persistence Of Mulato Ii In Florida, Joao M. B. Vendramini, Lynn E. Sollenberger, Graham C. Lamb, Maria L. Silveira

IGC Proceedings (1977-2023)

Grasses in the Brachiaria genus are the most widely grown forages in tropical America, occupying over 80 Mha (Boddey et al. 2004). Mulato II is apomictic and a vigorous, semi-erect cultivar resulting from 3 generations of crosses including original crosses between ruzigrass and signal-grass (cv. Basilisk, apomictic tetrapliod). According to Peters et al. (2003), Mulato produced 25% more herbage mass than palisadegrass (Brachiaria brizantha) and koroni-viagrass (Brachiaria humidicola) under similar management practices. Although Mulato II shows promise as a forage in tropical regions, herbage accumulation and persistence in subtropical areas is unknown. This publication …

Agronomic And Nutritional Evaluation Of Intraspecific Crosses In Brachiaria Decumbens, Simony A. Mendonça, Sanzio C. L. Barrios, Ulisses J. De Figueiredo, Geovani F. Alves, Cacilda B. Valle

IGC Proceedings (1977-2023)

Brachiaria decumbens cv. Basilisk is the single most important forage grass used for pastures in the tropics. This cultivar has exceptional adaptation to acid soils, vigorous growth, ease of establishment, and good forage value throughout the year, but these favourable characteristics are counteracted by its susceptibility to insect pests such as spittlebug. Breeding to produce improved cultivars within this species was impossible until 2009 due to the lack of compatible sexual ecotypes. With the success of somatic chromosome duplication of sexually reproducing diploid plants of B. decumbens (Simioni and Valle 2009), intraspecific crosses with natural apomictic tetraploid accessions were finally …

Reciprocal Recurrent Selection In The Breeding Of Brachiaria Decumbens, Sanzio C. L. Barrios, Cacilda B. Valle, Geovani F. Alves, Rosangela M. Simeão, Liana Jank

IGC Proceedings (1977-2023)

Pastures of Brachiaria decumbens cv. Basilisk radically changed the scenario of livestock production in central Brazil in the early 1970s and in fact, promoted the development of this vast region. However, despite the reasonable biomass yields and nutritional value when grown on these tropical acid soils, its susceptibility to grassland spittlebugs has limited its use. The breeding of B. decumbens in Brazil has been restricted to interspecific crosses using cv. Basilisk as a pollen donor due to the lack of compatible sexual ecotypes within this species. Recently, the successful chromosome duplication of a sexually reproducing diploid accession produced 3 successful …

Status Of Animal Feed Resources In Rwanda, M. Mutimura, A. B. Lussa, J. Mutabazi, C. B. Myambi, R. A. Cyamweshi, C. Ebong

IGC Proceedings (1977-2023)

Animal feed resources remain a major constraint for livestock development in tropical Africa. In Rwanda, grazing lands are shrinking sharply because crop cultivation is progressively encroaching on grazing areas with increasing human pressure (Mutimura and Everson 2011). Therefore, over 60% of households cultivating less than 0.7 ha, and owning livestock, practise zero-grazing, where farmers cut-and-carry forage and crop residues to feed animals that are kept exclusively under sheds (MINAGRI 2009). In general, the main feed for dairy cattle under a zero-grazing system is Napier grass (Pennisetum purpureum). For more than a decade, efforts to improve the feed resource …

Brachiaria Hybrids: Potential, Forage Use And Seed Yield, Esteban A. Pizarro, Michael D. Hare, Mpenzi Mutimura, Changjun Bai

IGC Proceedings (1977-2023)

A brachiaria breeding program initiated in 1988 at CIAT (Centro Internacional de Agricultura Tropical) combined desirable attributes found in accessions of Brachiaria brizantha and B. decumbens. Three apomictic hybrids have been released (cvs. Mulato, Mulato II and Cayman). Mulato showed agronomic potential but seed yields were low. Trials in Central America demonstrated the superiority of Mulato II, a vigorous grass with very deep and branched roots, giving it excellent drought resistance in the Brazilian Cerrado and Mexico. Mulato II has excellent nutritional value. Following trials in Mexico and Thailand, evaluating 155 new hybrids for 7 years, cv. Cayman was …

Molecular Genetic Variability, Population Structure And Mating System In Tropical Forages, Melissa Garcia, Bianca B. Z. Vigna, Adna C. B. Sousa, Letícia Jungmann, Fernanda W. Cidade, Guilherme Toledo-Silva, Patricia M. Francisco, Lucimara Chiari, Marcelo A. Carvalho, Claudio T. Karia, Fabio G. Faleiro, Rodolfo Godoy, M. Dall’Agnol, Sueli S. Pagliarini, Francisco H. D. Souza, Tatiana T. Souza-Chies, Liana Jank, Rosangela M. S. Resende, Cacilda B. Valle, Maria I. Zucchi, Anete P. Souza

IGC Proceedings (1977-2023)

Microsatellite (SSR) markers were developed for the following tropical forage species, using accessions available from the plant genetic resources (PGR) collections held by EMBRAPA (Brazilian Agricultural Research Corporation): Brachiaria brizantha, B. humidicola, Panicum maximum, Paspalum spp., Stylosanthes capitata, S. guianensis, S. macrocephala, Calopogonium mucunoides and Centrosema spp. The markers were used to analyse population structure and genetic diversity, evolution and origin of the genetic variability in the centre of origin, mating systems and genetic resources in EMBRAPA’s germplasm bank. The results shed light on the amount of genetic variation within and between populations, revealed …

Leucaena Toxicity: A New Perspective On The Most Widely Used Forage Tree Legume, Michael J. Halliday, Jagadish Padmanabha, Chris S. Mcsweeney, Graham L. Kerven, H. Max Shelton

IGC Proceedings (1977-2023)

The tree legume Leucaena leucocephala (leucaena) is a high quality ruminant feed, vitally important for livestock production in the tropics despite the presence of mimosine in the leaves. This toxic non-protein amino acid has the potential to limit productivity and adversely affect the health of animals. The discovery and subsequent distribution in Australia of the ruminal bacterium Synergistes jonesii as an oral inoculum was shown in the 1980s to overcome these toxic effects. However, recent surveys of the status of toxicity worldwide; improved understanding of the chemistry and mode of action of the toxins; new techniques for molecular sequencing; and …

Influence Of Biosolids Upon The Uptake Of Mn And Cd By Radish (Raphanus Sativus L.), Oluwadamilola O. Fateru

Electronic Theses and Dissertations

The influence of biosolids upon the uptake of Mn and Cd by radish (Raphanus sativus L.) was investigated through the characterization of biosolids, sequential extraction of the biosolids, and the determination of the metal content in the root, shoot and leaves of radish (Raphanus sativus L.).The biosolid samples from Nacogdoches Wastewater Sludge (NWWS), Lufkin Wastewater Sludge (LWWS), Soil Therapy Compost (STC) had pHs between 5.33 – 6.74. The elemental compositions of the biosolid samples were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES) and SEM/EDX. Major elements (K, Mg, Mn, P) needed for plant growth …

Late Jurassic Dinosaurs On The Move, Gastroliths And Long-Distance Migration, Josh Malone

Geography: Student Scholarship & Creative Works

The Jurassic Morrison Formation of the Rocky Mountain and Colorado Plateau regions is famous for its dinosaur fossils. The Morrison Formation (150-157 Ma) is comprised mostly of sandstone and mudstone that was deposited in a terrestrial deposition system that included fluvial, paludal, and lacustrine environments. Paleocurrent data indicates that Morrison sediment was transported to the north and east. Within the Morrison Formation, we find exotic pebble and cobble-size durable clasts of quartzite, chert and vein quartz weathering out of the mudstone paleosols. We interpret these exotic clasts as gastroliths, carried within the gastric mills of dinosaurs. For this study, we …

Global Impacts From Improved Tropical Forages: A Meta-Analysis Revealing Overlooked Benefits And Costs, Evolving Values And New Priorities, Douglas S. White, Michael Peters, Peter Horne

IGC Proceedings (1977-2023)

The wider use and improved performance of planted tropical forages can substantially change social, economic and environmental landscapes. By reviewing impact-related studies published in the past two decades, this paper shows how evolving development priorities have influenced the types of impacts being documented. A meta-analysis was used to examine 98 studies according to: (1) breadth of reported effects, as related to development goals of social equity, economic growth and environmental sustainability; (2) extent of effects, ranging from intermediate to longer-term impacts; and (3) measurement precision (identification, description and quantification). Impacts have been assessed for fewer than half of the documented …

Response Of Axonopus Catarinensis And Arachis Pintoi To Shade Conditions, A. N. A. Pachas, E. J. Jacobo, M. C. Goldfarb, S. M. Lacorte

IGC Proceedings (1977-2023)

In the north-east of Argentina, there are more than 100,000 hectares of silvopastoral systems where trees, forages and livestock are combined with the goal to diversify income, reduce financial risk, obtain more profit and enhance environmental benefit (Cubbage et al. 2013). The rapid adoption of this production system by farmers has generated high demand for information on shade tolerant grass and legume forage species.

Axonopus catarinensis is a native grass from Itajai Vally (Brazil) that was introduced to the north-east of Argentina 10 years ago; whereas Arachis pintoi is a sub-tropical legume (also native to Brazil) adapted to acid …

The Growth Response Of Tropical And Sub-Tropical Forage Species To Increasing Salinity, Hayley E. Giles, Christopher J. Lambrides, Scott A. Dalzell, David C. Macfarlane, H. Max Shelton

IGC Proceedings (1977-2023)

There is currently a growing coal seam gas (CSG) industry in Queensland, Australia. The industry requires beneficial-use strategies to consume the significant volumes of water released during CSG extraction. Irrigation of tropical and sub-tropical forage species for beef production is one option, however coal seam (CS) water is of varying quality due to moderate to high salinity and alkalinity. The application of chemically amended CS water over time could potentially increase soil salinity, which is known to reduce plant biomass production. While there were studies of salinity tolerance of many tropical and sub-tropical forage species 30 years ago, there is …

Performance Of Some Forages Species (Festuca Arundinacea L., Chloris Gayana Var. Katambora, Lotus Corniculatus L. And Medicago Sativa L.) In Saline Soil, Süleyman Temel, Uğur Şimşek, Bilal Keskin, İbrahim H. Yılmaz

IGC Proceedings (1977-2023)

Salinity is a soil degradation process which reduces plant diversity and agricultural productivity, decreases fertility and devalues the land in regions with arid and semi-arid climate. This process inhibits water and nutrient intake of plants from soil due to changeable nitrogen percentage and/or the intensity of soluble salt concentration. When the fact that agricultural lands are limited around the world and that the need for nutrition increases incrementally is taken into consideration, it is obvious that available lands should be used more effectively. Hence, it is quite crucial to reclaim saline soil and utilize it more economically (Woods 1996).

Plantain (Plantago Lanceolata) Outperforms Chicory (Cichorium Intybus) Under Moisture Stress In Glasshouse, Lydia M. Cave, Peter D. Kemp, Paul R. Kenyon, Stephen T. Morris

IGC Proceedings (1977-2023)

Forage chicory (Cichorium intybus) and plantain (Plantago lanceolata) are now widely used throughout the world as high feed quality perennial herbage (Sanderson et al. 2003; Labreveux et al. 2006; Li et al. 2010; Golding et al. 2011; Hutton et al. 2011). Both are taprooted plants and are thus likely to confer a degree of drought tolerance through accessing water deeper in the soil profile (Kemp et al. 2010). Nie et al. (2008) reported chicory can tolerate moisture stress to a greater degree than plantain. However, overall little is known …

Total Phenolic And Flavonoid Contents Of Anacyclus Pyrethrum Link Plant Extracts And Their Antioxidant Activity, Hanane Elazzouzi, Nadia Zekri, Touriya Zair, Mohamed Alaoui El Belghiti

Karbala International Journal of Modern Science

In this study, Anacyclus pyrethrm Link (roots) is a medicinal plant from the Asteraceae family, endemic to Morocco and is widely used in traditional medicine; was investigated for their total phenolics, flavonoids contents and antioxidant activities. The quantitative estimation of total flavonoids and phenols by the colorimetric method showed that the extracts were prepared by fractionation of the crude hydromethanolic extract (ethyl acetate and n-butanol). The phenol content of various extracts, estimated by the Folin-Ciocalteu method, was between 1,44 and 12,82 mg EAG / g extract of A. pyrethrum. Moreover, it is clear that ethyl acetate was the …

Inclusion Of Kinetic Proteomics In Multi-Omics Methods To Analyze Calorie Restriction Effects On Aging, Richard Hajime Carson

Theses and Dissertations

One of the greatest risk factors for disease is advanced age. As the human lifespan has increased, so too have the burdens of caring for an increasingly older population suffering from rising rates of cardiovascular disease, kidney disease, diabetes, and dementia. The need for improving medical technology and developing new therapies for age-related diseases is manifest. Yet our understanding of the processes of aging and how to attenuate the effects of aging remains incomplete. Various studies have established calorie restriction as a robust method for extending lifespan in laboratory organisms; however the mechanism is a topic of much debate. Advancing …

Validating And Extending The National Better Fertiliser Decisions For Pasture Critical Values For Phosphorus, David Rogers, David Weaver, Ron Master, Robert Summers

The State of Soil Science in WA

Viable pasture based grazing systems in south-western Australia have been made possible over much of the landscape through input of nutrients to soils which were impoverished when first cleared. Initial dramatic increases in production stimulated subsequent applications of fertilisers, particularly phosphatic fertilisers, ever since. However, excessive fertiliser application can be tied to environmental impacts including the loss of phosphorus (P) to waterways impacting on water quality.

Analysis Of E-Cigarette Liquids And Aerosols By Nmr Spectroscopy: Compositions, Boiling Points, And Degradation Profiles, Anna Katherine Duell

Dissertations and Theses

Electronic cigarettes (e-cigarettes) are an increasingly popular tobacco product used by ~3% of adults and 21% of 12th grade students (as reported for the prior 30-days in surveys) in the United States in 2018. As of 2019, this number went up to ~25% for 12th grade students. Due to how quickly e-cigarettes gained popularity and the rapid design changes that have ensued, there has not been enough time to accurately assess the effects of e-cigarettes, particularly for long-term use. The liquids used in e-cigarettes (e-liquids) can degrade when vaped in an e-cigarette, so understanding the variables that can modulate degradation …

Chronic Muscle Weakness And Mitochondrial Dysfunction In The Absence Of Sustained Atrophy In A Preclinical Sepsis Model, Allison M. Owen, Samir P. Patel, Jeffrey D. Smith, Beverly K. Balasuriya, Stephanie F. Mori, Gregory S. Hawk, Arnold J. Stromberg, Naohide Kuriyama, Masao Kaneki, Alexander G. Rabchevsky, Timothy A. Butterfield, Karyn A. Esser, Charlotte A. Peterson, Marlene E. Starr, Hiroshi Saito

Physiology Faculty Publications

Chronic critical illness is a global clinical issue affecting millions of sepsis survivors annually. Survivors report chronic skeletal muscle weakness and development of new functional limitations that persist for years. To delineate mechanisms of sepsis-induced chronic weakness, we first surpassed a critical barrier by establishing a murine model of sepsis with ICU-like interventions that allows for the study of survivors. We show that sepsis survivors have profound weakness for at least 1 month, even after recovery of muscle mass. Abnormal mitochondrial ultrastructure, impaired respiration and electron transport chain activities, and persistent protein oxidative damage were evident in the muscle of …