Digital Commons Network^™

Simultaneous Extraction And Quantitative Analysis Of S-Methyl-L-Cysteine Sulfoxide, Sulforaphane And Glucosinolates In Cruciferous Vegetables By Liquid Chromatography Mass Spectrometry, Armaghan Shafaei, Caroline R. Hill, Jonathan M. Hodgson, Lauren C. Blekkenhorst, Mary C. Boyce

Research outputs 2022 to 2026

Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-L-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more …

Comparison Of Raw Accelerometry Data From Actigraph, Apple Watch, Garmin, And Fitbit Using A Mechanical Shaker Table, James W. White Iii, Olivia L. Finnegan, Nick Tindall, Srihari Nelakuditi, David E. Brown Iii, Russell R. Pate Ph.D., Gregory J. Welk, Massimiliano De Zambotti, Rahul Ghosal, Yuan Wang, Sarah Burkart, Elizabeth L. Adams, Mvs Chandrashekhar, Bridget Armstrong, Michael W. Beets, Robert Weaver Med, Phd

Faculty Publications

The purpose of this study was to evaluate the reliability and validity of the raw accelerometry output from research-grade and consumer wearable devices compared to accelerations produced by a mechanical shaker table. Raw accelerometry data from a total of 40 devices (i.e., n = 10 ActiGraph wGT3X-BT, n = 10 Apple Watch Series 7, n = 10 Garmin Vivoactive 4S, and n = 10 Fitbit Sense) were compared to reference accelerations produced by an orbital shaker table at speeds ranging from 0.6 Hz (4.4 milligravity-mg) to 3.2 Hz (124.7mg). Two-way random effects absolute intraclass correlation coefficients (ICC) tested inter-device reliability. …

High Dietary Folic Acid Supplementation Reduced The Composition Of Fatty Acids And Amino Acids In Fortified Eggs, Ao-Chuan Yu, Yu-Han Deng, Cheng Long, Xi-Hui Sheng, Xiang-Guo Wang, Long-Fei Xiao, Xue-Ze Lv, Xiang-Ning Chen, Li Chen, Xiao-Long Qi

Faculty, Staff and Student Publications

AIMS: The study aimed to evaluate the effects of dietary folic acid (FA) on the production performance of laying hens, egg quality, and the nutritional differences between eggs fortified with FA and ordinary eggs.

METHODS: A total of 288 26-week-old Hy-Line Brown laying hens (initial body weights 1.65 ± 0.10 kg) with a similar weight and genetic background were used. A completely randomized design divided the birds into a control group and three treatment groups. Each group consisted of six replicates, with twelve chickens per replicate. Initially, all birds were fed a basal diet for 1 week. Subsequently, they were …

Grazing Management Of Tagasaste (Chamaecytisus Proliferus) For Sheep And Cattle Production In Southern Australia, N J. Edwards, G M. Allen, D M. Mcneill, C M. Oldham

IGC Proceedings (1977-2023)

Direct grazing of hedgerows of tagasaste (Chamaecytisus proliferus) by sheep or cattle appear to be very robust systems. Tagasaste persists under a continuous grazing regime with cattle such that plant regrowth maintained between 5 and 10 cm in length produces in excess of 215 kg of animal liveweight/ha/year. This level of production is also sustained within a rotational grazing regime. Under both grazing systems cattle production within a year is highly seasonal, with liveweight gains from young cattle peaking at 1.0-1.5 kg/head/day in winter and spring, but dropping to maintenance only by late summer-autumn. Sheep, like cattle, can …

Shrub Palatability To Rusa Deer (Cervus Timorensis Russa) In New Caledonia, C Corniaux, S Le Bel, J M. Sarrailh

IGC Proceedings (1977-2023)

The objective of this study was to determine a palatability scale of five shrub legumes to rusa deer during the dry season in New Caledonia. Acacia ampliceps and Samanea saman remain low in acceptability. Gliricidia sepium is more palatable but quite less than Leucaena leucocephala (native cultivar) and Calliandra calothyrsus (San Ramon). Therefore, since the regression of Leucaena leucocephala population, Calliandra calothyrsus could be very promising to replace it in the deer diet. On the other hand, Acacia ampliceps seems to be the most interesting shrub legume to plant in the west coast, where soil erosion, due to successive droughts …

Fodder Production Of Multipurpose Trees In A Maize Farming System Of Subhumid Southern Africa, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Ways to integrate fodder production into maize production systems under Southern Africa’s subhumid conditions have been studied. One way involves the planting of tree and grass fodder hedges along soil conservation contour-bunds in maize fields. Management factors that influence the productivity of maize and fodder components in the system are fodder spp., side-pruning and deferment of wet season cutting. The other option involves interplanting tree fodders with maize through the use of a coppicing tree such as Acacia angustissima. Practical applications of these methods in mixed crop-livestock production systems are discussed.

Differences In Chemical Composition Among Provenances Of Browse Species In A Subhumid Environment: Relation To Use As Supplements, B H. Dzowela, L Hove, P L. Mafongoya

IGC Proceedings (1977-2023)

Studies were initiated in a subhumid Southern African environment to assess the chemical composition and nutritive value of Acacia angustissima (Mill.) Kuntze, Calliandra calothyrsus Meiss. and Leucaena species. The objective was to determine variability in crude protein (CP) concentration, acid detergent fibre (ADF), neutral detergent fibre (NDF), and soluble and insoluble proanthocyanidins from fodder samples of species, subspecies and provenances. A wide range was found in these parameters. The implications of these chemical composition factors, especially proanthocyanidins, on the use of these browse fodders in livestock feeding systems are discussed.

Tree Forages As Nitrogen Source For Ruminants In Sahelian Agro-Silvipastoral Systems, S T. Fall, B M. Doreau

IGC Proceedings (1977-2023)

Tree forages contribute up to 80% DM and 100% nitrogen content in the ruminants diet in the Sahel during the dry season. However, crude protein (CP) concentration in browse within a wide range and variation factors remain to be elucidated for better integration of browses in ruminants feeding systems. Variation factors of CP content and in vivo digestibility of four Sahelian browse species were investigated to appreciate the potential as nitrogen sources in Sahelian ruminant feeding systems. Seasonal variations in nitrogen content were evaluated in different plants part of A. raddiana, B. rufescens, B. aegyptiaca and G. senegalensis harvested in …

Evaluation Of Animal Productivity In A Sustainable Grazing System Based On The Use Of Trees In Association With Pastures, J M. Iglesias Gomez

IGC Proceedings (1977-2023)

Young bulls of different breeds ( Zebu, Holstein and F-1 strain Holstein Zebu) were grazing together on a meadow composed of Guinea grass (Panicum maximum), Brachiaria (Brachiaria decumbens) and a mixture of natural pastures, all in association with Leucaena (Leucaena leucocephala) sown at a density of 555 plants per ha in order to evaluate the potential of that system for beef production. A completely randomized design was used. The meadow was rotationally grazed at stocking rate of 3.6 animal per ha. During the 18 months of fattening the best results were obtained with the Zebu type. …

Changes In Understorey Pasture Composition In Agroforestry Regimes In New Zealand, M F. Hawke, A G. Gillingham

IGC Proceedings (1977-2023)

Long term Agroforestry trials were established in the North and South Islands of New Zealand between 1971-76. They compared a range of final tree stockings of Pinus radiata planted into pasture with open pasture control plots and were measured for tree growth parameters and agricultural production. This paper presents the results of pasture species changes over the period of tree age 10-22 years. Pasture species composition under Pinus radiata changed with time, dependent primarily on the rate of canopy closure. Changes occurred relatively rapidly in high tree stocked areas of 400 stems per hectare (sph) in the North Island trials …

Farmers' Screening Of Indigenous Fodder Trees And Shrubs In Kenya, R L. Roothaert, H K. Arimi, E N. Kamau

IGC Proceedings (1977-2023)

The lack of fodder quantity and quality is a constraint for small scale livestock production systems in central Kenya which are intensive or are rapidly becoming so. This study examined farmers’ knowledge about indigenous fodder trees and shrubs (IFTS) which are fed to livestock. The study consisted of a formal survey in three agroecological zones with mean annual rainfall varying from 750 to 1400 mm. Farmers rated IFTS for different qualities, which were analysed by Chi square. Differences of ratings among species for these qualities were significant (p<0.05). The most promising species for the sub-humid zone, in terms of what farmers want to plant and in terms of preliminary nutritive analysis, were: Tithonia diversifolia, Commiphora zimmermanii, Ficus spp. and Trema orientalis. The species …

Spatial Analysis Of Land Use By Cattle Herds In A Village Of The Sudanese Zone In Senegal. Application For Grazing System Improvement, A Ickowicz, J C. Usengumuremyi, D Bastien, N De Choudens

IGC Proceedings (1977-2023)

Spatial analysis of land use by cattle herds in the sub-humid area of Senegal is conducted through the utilisation of a Geographic Information System. This tool allows us to establish relationships between spatial practices, ruminant nutrition and performances. It gives leads to proposals for the improvement of the extensive ruminant feeding system.

Using Cuticular Wax Alkanes And Computer Simulation To Estimate Diet Selection, Herbage Intake And Nutrient Cycling In Grazing Sheep, H Dove, R J. Simpson

IGC Proceedings (1977-2023)

In grazing ewes, plant cuticular wax alkanes were used as markers to estimate diet selection, herbage intake, N intake and N excretion in faeces. Pasture and animal data were then used as inputs to the decision-support system GrazFeed, which simulates grazing and digestion to predict herbage intake, N intake and N excretion. Estimated and predicted intakes agreed closely, especially for N intake, and it is concluded that, subject to further investigation of the possibility that GrazFeed slightly under-estimated faecal N excretion, the close agreement between estimated and predicted OM and N intakes suggests that this combined use of alkane methods …

Foraging Behavior Of Zebu Cattle Grazing Deciduous Forest, L Ortega, J Castillo, F Rivas

IGC Proceedings (1977-2023)

The objective of this study was to determine whether the foraging behavior of Zebu cattle in deciduous forest was affected by time of the year. Data were recorded during the wet, nortes and dry seasons. Ten Zebu cows were used to record biting rate. Intake and bite size was estimated using three esophageally fistulated animals. Cattle browsed shrubs and trees in the wet season and ingested litter leaves in the dry season which resulted in differences of ingestive behavior. In general, bite size and intake were higher in the wet season and decreased during the nortes and dry seasons.

Characterization Of Grazing Patterns In Cool-Season Annual And Warm-Season Perennial Grass Pastures, G E. Aiken, M S. West, D I. Bransby

IGC Proceedings (1977-2023)

Pastures are often either under- or over-grazed, which can affect both pasture sustainability and livestock performance. Disk meter heights were measured for rye (Secale cereale L.) -ryegrass (Lolium L. multiflorum Lam.) pasture to characterize stocking rate (2.5, 3.7, and 4.9 steers/ha) effects on grazing patterns. In another study, grazing indices were calculated for bermudagrass [Cynodon dactylon (L.) Pers.] to describe pasture utilization over time for three stocking rates (3.0, 5.9, 8.9 steers/ha). Distributions of DMH for rye-ryegrass showed distinct grazing patterns for each stocking rate, with the distributions changing from bimodal to multi-modal from heavy to light …

Relationships Between The Nutrient Content Of Irrigated Pasture On Offer And That Selected By Grazing Dairy Cows, W J. Wales, C R. Stockdale, P T. Doyle

IGC Proceedings (1977-2023)

An experiment was conducted to determine the dry matter, energy, crude protein and fibre intake by dairy cows in late lactation when grazing perennial pasture offered at allocations of 15, 20, 30 and 40 kg DM/cow.day. The cows consistently selected a diet 10% higher in digestibility than that on offer. In contrast, cows selected diets with crude protein levels increasing from 22% to 40%, above that in pasture on offer, as pasture allocation increased. A similar pattern was observed for the intake of white clover (Trifolium repens L). The increase in the concentration of crude protein in the diet …

Species Preference Influences On Cattle Grazing Behaviour, G P. Cosgrove, C B. Anderson, R H. Fletcher

IGC Proceedings (1977-2023)

Lotus corniculatus offers specific nutritional benefits to animals, but exploiting these advantages in grazing systems depends on the proportion of lotus in the feed offered and the animals' preference, hence desire to select for it. To determine preference for lotus, heifers were offered free-choice in contrasting, spatially separated but adjacent monocultures of ryegrass-lotus or red clover-lotus. Following a one-week period to adjust to the species offered and their arrangement, 10 young heifers were observed at 10-minute intervals during daylight hours, and the species they were on and whether or not they were grazing was recorded. This procedure was conducted in …

Influence Of Vegetation Patch Characteristics On Discriminatory Grazing By Dairy Cows, W M. Griffiths, J Hodgson, G C. Arnold, C W. Homes

IGC Proceedings (1977-2023)

Two studies involving the sequential grazing of sets of patches on a perennial ryegrass (Lolium perenne) sward were carried out to investigate the effects of patch characteristics on the selective behaviour of grazing cattle. Experiment 1 involved a range of combinations of sward height (8.9 - 19.6 cm) and bulk density (1.33 - 1.67 mg DM/cm3). Distribution of grazing activity (number of bites or residence time) was strongly and positively related to patch height, but additional effects of variation in bulk density were limited. In Experiment 2 patches were manipulated to offer contrasts in both height (13.4 and …

Shade For Cattle On Pasture: A Guide For Ontario Producers, E J. Finegan, T M. Widowski

IGC Proceedings (1977-2023)

In tropical climates, adequate shade may be essential to ensure the survival and productivity of grazing animals, but in the temperate Ontario climate, the necessity or benefits of providing shade for grazing cattle are less clear, and have proved controversial. Meteorological data indicate that beef cattle in Ontario may be at risk of suffering from heat stress on more than 25% of days during July and August, and may decrease feed intake and benefit from the provision of shade for more than 50% of days during the two hottest summer months. A booklet, entitled ‘Beat the Heat. A Guide …

Automatic Image-Based Nutritional Calculator App, Kejvi Cupa

USF Tampa Graduate Theses and Dissertations

Nutrition plays a pivotal role in shaping an individuals’ health and quality of life, making the evaluation of dietary intake crucial for promoting healthier lifestyle choices. Various solutions, particularly mobile apps, have been developed to facilitate the process of dietary estimation. Accurate nutritional intake assessment relies on two key components: ingredient recognition and food portion estimation. For a mobile app to offer a comprehensive solution for automatic nutritional assessment, it must address both components.

In this work, we focus on a mobile app pipeline: the semi-automatic pipeline which focuses on automatic food ingredient recognition. This pipeline integrates state-of-the-art models for …

An Exposome Atlas Of Serum Reveals The Risk Of Chronic Diseases In The Chinese Population, Lei You, Jing Kou, Mengdie Wang, Guoqin Ji, Xiang Li, Chang Su, Fujian Zheng, Mingye Zhang, Yuting Wang, Tiantian Chen, Ting Li, Lina Zhou, Xianzhe Shi, Chunxia Zhao, Xinyu Liu, Surong Mei, Guowang Xu

Faculty, Staff and Student Publications

Although adverse environmental exposures are considered a major cause of chronic diseases, current studies provide limited information on real-world chemical exposures and related risks. For this study, we collected serum samples from 5696 healthy people and patients, including those with 12 chronic diseases, in China and completed serum biomonitoring including 267 chemicals via gas and liquid chromatography-tandem mass spectrometry. Seventy-four highly frequently detected exposures were used for exposure characterization and risk analysis. The results show that region is the most critical factor influencing human exposure levels, followed by age. Organochlorine pesticides and perfluoroalkyl substances are associated with multiple chronic diseases, …

Screening Ryegrass Cultivars For Aluminium Sensitivity In Nutrient Soluations, F O. Gallardo, S Benavides, R Demanet, F Borie

IGC Proceedings (1977-2023)

The objective of this study was to evaluate Al and H+ tolerance of nine ryegrass (Lolium perenne and L. multiflorum) cultivars by growing plants in a mineral solution (pH 4.8 and 6.0 and 0-200 (M Al). Results showed that Yatsyn-1, with a relative root length of 91 was the cultivar least affected by acidity (H+), and Concord ( RRLpH = 58) was the cultivar with the highest H+ sensitivity. In connection with Al-tolerance, Yatsyn-1 and Concord, were the most tolerant cultivars with 88 and 87 RRLAl, respectively whereas Nui and Marathon, were the most sensitive ones, reaching only …

Use Of Flow Cytometer In Breeding Festuca X Lolium Hybrids, M C. Kerlan, M Ghesquiere

IGC Proceedings (1977-2023)

Ploidy in various generic Lolium - Festuca progenies were assessed by flow cytometry and compared to conventional chromosome counting. In non-segregating triploid and tetraploid progenies, the cytometer estimated so accurately the level of plant ploidy that chromosomes counting would be no use. In segregant progenies, all the tetraploid plants according to the cytometer had effectively 28 chromosomes. Among the triploid and pentaploid plants detected by the cytometer, 40% of the plants were tetraploid. They represented only 29% of the progeny which must be counted. The flow cytometry can be used in routine interspecific breeding programmes. Conventional chromosome counting should be …

Selection Of Interspecific Hybrids Of Brachiaria-A Tropical Forage Grass, C B. Do Valle

IGC Proceedings (1977-2023)

The aim of the breeding program at the National Beef Cattle Research Center (CNPGC) of the Brazilian Corporation for Agricultural Research (EMBRAPA), is to produce vigorous apomictic hybrids with wide edaphic adaptation, good production and nutritive value combined with spittlebug (Homoptera:Cercopidea) resistance. Fiftysix superior hybrids were selected based on visual estimates of vigor, leafiness, tolerance to drought and natural spittlebug infestation in the field. These were characterized for mode of reproduction, spittlebug reaction under controlled conditions and for morphological diversity using nine selected traits. Multivariate analysis classified the population into seven groups where the hybrids were clearly intermediate in plant …

Lc-Ms/Ms Reveals Hordeins Are Enriched In Brewers’ Spent Grain, Mitchell G. Nye-Wood, Michelle L. Colgrave

Research outputs 2022 to 2026

Barley is commonly used in malting and brewing, and spent grain is repurposed for other foods. Barley contains gluten proteins called hordeins that cause intestinal damage and disease symptoms if eaten by people with celiac disease and related conditions. While the mashing process in brewing can partially hydrolyze immunogenic epitopes in hordeins, the immunogenic epitope load between the starting malt and spent grain has not been investigated. Herein, we quantified hordeins in commercially available spent grain and from matching malt. Liquid chromatography-mass spectrometry (LC-MS) and sandwich and competitive R5 ELISAs were used for quantification, revealing a higher abundance of gluten …

Introduction Of Vegetative And Reproductive Characters Into Trifolium Repens By Interspecific Hybridisation, A H. Marshall, T P.T Michaelson-Yeates, M Meredith, D H. Hides

IGC Proceedings (1977-2023)

Interspecific hybridization between white clover (Trifolium repens L.) and ball clover (T.nigrescens) and Caucasian clover (T.ambiguum) is a means of improving the reproductive growth and persistency of T.repens. F1 hybrids between T.repens and T.nigrescens were produced without ovule culture and confirmed as true hybrids by chromosome counts, isoenzyme analysis and leaf markers. Their growth habit was generally intermediate between the two parents. After backcrossing to T.repens the BC1 and BC2 hybrids had more of the characteristics associated with T.repens though they retained some of the greater reproductive growth of …

Plants Reduced Nitrous Oxide Emissions From A Northern Great Plains Saline/Sodic Soil, Sharon A. Clay, Thandiwe Nleya, David E. Clay, Deepak Joshi, Dwarika Bhattarai, Shin-Yi Marzano, Bhanu Prakash Petla

Agronomy, Horticulture and Plant Science Faculty Publications

The slowly establishing salt-tolerant perennial grasses reduced nitrous oxide (N2 O- N) emissions from saline/sodic soil compared to barren areas. Other salt-tolerant species may accelerate vegetative establishment and reduce N 2 O-N emissions. In a greenhouse study, barley (Hordeum vulgare L.), Florida broadleaf mustard (Brassica juncea L.), and Kernza intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] were grown for 63 days to compare shoot biomass and chemical composition, N 2 O-N emissions, and the soil microbiome between saline/sodic and productive (non-salt impacted) soils. Emissions were measured six times daily from 1 to 22 and 42 to 63 …

Maine Career Exploration: Interim Evaluation Report, Sydney Brown, Julia Bergeron-Smith Mppm, Msw, Sarah Goan, Madison Burke, Becky Wurwarg

Publications

The Maine Career Exploration (MCE) Program is a two-year pilot initiative designed to connect young people with Maine’s economy through hands-on, age-appropriate career exploration experiences. Launched in 2022 with funding from Governor Janet Mills’ Jobs and Recovery Plan, the program seeks to provide meaningful paid work experiences, expand career exploration programming in schools and communities, and create sustainable infrastructure to support long-term career readiness opportunities across the state. Managed by the Maine Department of Economic and Community Development, the initiative invests in three main areas: the Maine Children’s Cabinet Career Exploration Pilot Project, the Maine Department of Education’s Extended Learning …

Pollinator Communities And Their Ecosystem Services At Conservation Grasslands And Adjacent Croplands, Araceli Gomez Villegas

Department of Entomology: Dissertations, Theses, and Student Research

Pollinators are intrinsically linked to the success of unmanaged and managed ecosystems by providing pollination services that aid in the reproduction of wildflowers and many crops. Land use change, habitat loss, fragmentation, and related landscape-level phenomena (for example, increased pesticide exposure) threaten pollinators and have been associated with population declines. In the Midwestern region of the United States, land conversion of native prairies and grasslands to row-crop agriculture has been one of the largest contributors to pollinator habitat loss. Conservation programs, such as the Conservation Reserve Program, have worked towards removing environmentally sensitive lands from agriculture production and enrolling them …

Association Between Short Birth Spacing And Child Malnutrition In Bangladesh: A Propensity Score Matching Approach, Foyez Ahmmed, Nahid Hasan, Faruk Hossain, Tareq Ferdous Khan

Faculty Publications

Objectives: This study aimed to explore the effects of short birth spacing (SBS), which is defined as a period of less than 33 months between two successive births, on multiple concurrent forms of child malnutrition (MCFCM) and at least one form of child malnutrition (ALOFCM) using propensity score matching (PSM). Methods: This study used data extracted from the 2017- 18 Bangladesh Demographic and Health Survey. PSM with four different distance functions, including logistic regression, classification and regression tree, single hidden layer neural network and random forest, were performed to evaluate the effects of SBS on MCFCM and ALOFCM. We also …