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An Ethnobotanical, Ecological And Lc-Ms-Based Chemometric Investigation Of Phaleria Nisidai, A Traditional Adaptogen Containing Diterpene Esters From Palau, Micronesia, Daniel Kulakowski

Dissertations, Theses, and Capstone Projects

An ethnobotanical, ecological and LC-MS-based chemometric investigation of Phaleria nisidai, a traditional adaptogen containing diterpene esters from Palau, Micronesia

Palau is a country with a rich heritage of traditional medicine still being practiced. One of the most popular and respected remedies in Palau is a tea made from fresh leaves of Phaleria nisidai Kaneh. (Thymelaeaceae). Interviews conducted to determine the use of this plant revealed that it is employed non-specifically to treat a variety of general health concerns. Its use as a prophylactic to keep away sickness, as a 'system cleaner', as well as for strength and energy indicate …

An Experimental Investigation Into The Mechanisms Of Bacterial Evolution, Zhenmao Wan

Dissertations, Theses, and Capstone Projects

This thesis studies the two fundamental mechanisms of bacterial evolution — horizontal gene transfer and spontaneous mutation, in the bacterium Escherichia coli through novel experimental assays and mathematical simulations. First, I will develop a growth assay utilizing the quantitative polymerase chain reaction (qPCR) to provide real-time enumeration of genetic marker abundance within bacterial populations. Second, I will focus on horizontal gene transfer in E. coli occurring through a process called conjugation. By fitting the qPCR data to a resource limited, logistic growth model, I will obtain estimated values of several key parameters governing the dynamics of DNA transfer through conjugation …

Concomitant Socioeconomic, Behavioral, And Biological Factors Associated With The Disproportionate Hiv Infection Burden Among Black Men Who Have Sex With Men In 6 U.S. Cities, Kenneth H. Mayer, Lei Wang, Beryl A. Koblin, Sharon B. Mannheimer, Manya Magnus, +13 Additional Authors

Epidemiology Faculty Publications

Background

American Black men who have sex with men (MSM) are disproportionately affected by HIV, but the factors associated with this concentrated epidemic are not fully understood.

Methods

Black MSM were enrolled in 6 US cities to evaluate a multi-component prevention intervention, with the current analysis focusing on the correlates of being newly diagnosed with HIV compared to being HIV-uninfected or previously diagnosed with HIV.

Results

HPTN 061 enrolled 1553 Black MSM whose median age was 40; 30% self-identified exclusively as gay or homosexual, 29% exclusively as bisexual, and 3% as transgender. About 1/6th(16.2%) were previously diagnosed with HIV (PD); …

Antibiotic Compounds Isolated From Plant Microbes, Michael C. Haymore, Dr. Noel Owen

Journal of Undergraduate Research

The discovery of penicillin by Alexander Flemming in the early twentieth century is seen as one of the most significant medical advances in the past 100 years. Millions of people have had their lives preserved or prolonged due to the use of effective antibiotics since their discovery and wide-spread use. However, with the incredibly diverse and constantly evolving infectious diseases older antibiotics such as penicillin are becoming obsolete in the fight against infection. Thus, there remains a constant need to discover antibiotics effective against today’s diseases. Plants are a rich source of antibiotic compounds. Because plants live and compete in …

Is Hyperbaric Oxygen Therapy An Effective Treatment For Autism? A Review, Daniel Dunleavy, Bruce A. Thyer

Journal of Adolescent and Family Health

Objectives: We review outcome studies regarding the effectiveness of hyperbaric oxygen therapy (HBOT) for Autism Spectrum Disorders (ASD). Method: Studies were identified through electronic bibliographic databases and manual searches of article reference lists. Results: A total of 8 studies met eligibility criteria, consisting of three randomized controlled trials (RCTs), one quasi-experimental study involving a comparison group, two pre-experimental one-group pretest–posttest studies, and two single-system designs. Studies reviewed did not offer credible evidence to suggest that HBOT is an effective treatment for autism. Conclusion: It is premature to call HBOT an effective treatment for Autism and ASD. Individuals clinically treated with …

Change Point Testing In Logistic Regression Models With Interaction Term, Youyi Fong, Chongzhi Di, Sallie Permar

UW Biostatistics Working Paper Series

The threshold effect takes place in situations where the relationship between an outcome variable and a predictor variable changes as the predictor value crosses a certain threshold/change point. Threshold effects are often plausible in a complex biological system, especially in defining immune responses that are protective against infections such as HIV-1, which motivates the current work. We study two hypothesis testing problems in change point models. We first compare three different approaches to obtaining a p-value for the maximum of scores test in a logistic regression model with change point variable as a main effect. Next, we study the testing …

Assessing The Optimal Virulence Of Malaria‐Targeting Mosquito Pathogens: A Mathematical Study Of Engineered Metarhizium Anisopliae, Bernhard Konrad, Michael R. Lindstrom, Anja Gumpinger, Jielin Zhu, Daniel Coombs

School of Mathematical & Statistical Sciences Faculty Publications

Background

Metarhizium anisopliae is a naturally occurring fungal pathogen of mosquitoes. Recently, Metarhizium has been engineered to act against malaria by directly killing the disease agent within mosquito vectors and also effectively blocking onward transmission. It has been proposed that efforts should be made to minimize the virulence of the fungal pathogen, in order to slow the development of resistant mosquitoes following an actual deployment.

Results

Two mathematical models were developed and analysed to examine the efficacy of the fungal pathogen. It was found that, in many plausible scenarios, the best effects are achieved with a reduced or minimal pathogen …

Redefining Quality In Developing World Education, Kristi Jane Yuthas, Marc J. Epstein

Business Faculty Publications and Presentations

In the outskirts of Medellin, Colombia, impoverished rural schoolchildren have cause for hope. The Colombian Coffee Growers’ Association wants to hire them. Why? Because these children have developed the independent thinking, communication, and work skills that will make them an asset to the industry. They developed these skills in their multigrade primary schools, where children do most of their learning in competence-based groups, while the teacher functions as guide and coach.

In Kenya, a teenage boy is also celebrating. A primary school dropout who once survived outside the law, he now runs his own small business, lives on his own, …

Expression And Characterization Of The Novel Oxidase Virh-Ox, John Dronzek

Chemistry Honors Papers

No abstract provided.

Simultaneous High-Performance Liquid Chromatography-Tandem Mass Spectrometry (Hplc-Ms-Ms) Analysis Of Cyanide And Thiocyanate From Swine Plasma, Raj K. Bhandari, Erica Manandhar, Robert P. Oda, Gary A. Rockwood, Brian A. Logue

Chemistry and Biochemistry Faculty Publications

An analytical procedure for the simultaneous determination of cyanide and thiocyanate in swine plasma was developed and validated. Cyanide and thiocyanate were simultaneously analyzed by high-performance liquid chromatography tandem mass spectrometry in negative ionization mode after rapid and simple sample preparation. Isotopically labeled internal standards, Na¹³C¹⁵N and NaS¹³C¹⁵N, were mixed with swine plasma (spiked and nonspiked), proteins were precipitated with acetone, the samples were centrifuged, and the supernatant was removed and dried. The dried samples were reconstituted in 10 mM ammonium formate. Cyanide was reacted with naphthalene-2,3-dicarboxaldehyde and taurine to form N-substituted …

Herring : An Analysis Of Spawning Ground Management, Ecological Conditions And Human Impacts In Greifswald Bay, Vistula Lagoon And Hanö Bight., Dariusz P. Fey, Adam M. Lejk, Lena Szymanek, Iwona Psuty, Tomasz Nermer, Friederike Lempe, Harry V. Strehlow, Patrick Polte, Dorothee Moll, Nardine Stybel, Anne Hiller, Michael Van Laak, Henrik Nilsson, Lilitha Pongolini

Books

This book compiles the findings of the HERRING project which was conducted from 2012 until 2015 and part-financed by the EU South Baltic Programme.

The main objective of the HERRING project is to improve the consideration of including herring spawning grounds in coastal management. Herring as a resource recourse would be part of the economic development of coastal areas, and HERRING strongly emphasizes the importance of foster an integrated coastal management in the South Baltic Sea. Three case study areas in Germany, Poland and Sweden serve as the basis of the approach, which can be roughly distinguished in two parts. …

Functional Evolution Of Plp-Dependent Enzymes Based On Active-Site Structural Similarities, Jonathan Catazaro, Adam Caprez, Ashu Guru, David Swanson, Robert Powers

Holland Computing Center: Faculty Publications

Families of distantly related proteins typically have very low sequence identity, which hinders evolutionary analysis and functional annotation. Slowly evolving features of proteins, such as an active site, are therefore valuable for annotating putative and distantly related proteins. To date, a complete evolutionary analysis of the functional relationship of an entire enzyme family based on active-site structural similarities has not yet been undertaken. Pyridoxal-5’-phosphate (PLP) dependent enzymes are primordial enzymes that diversified in the last universal ancestor. Using the Comparison of Protein Active Site Structures (CPASS) software and database, we show that the active site structures of PLP-dependent enzymes can …

Proceedings Of The 126th Annual Meeting Of The Iowa Academy Of Science [Program, 2014], Iowa Academy Of Science

Iowa Academy of Science Documents

Contents:

Program Summary --- 2-3
Acknowledgements --- 4
Contents --- 5
Iowa Junior Academy of Science Competition --- 6
General Session I --- 7
Iowa Junior Academy of Science Award Luncheon --- 8
Iowa Academy of Science Business Meeting --- 8
Necrology --- 8
Special Events --- 9
Iowa Academy of Science 2012-2013 Volunteers --- 10-11
Symposium A --- 12
Symposium B --- 13
Symposium C --- 14
Senior Poster Session Program --- 15-19
Social Hour --- 20
President's Banquet --- 20-21
General Session II --- 22
General Session III --- 23
Excellence in Science Teaching Awards Luncheon --- 24 …

Engagement With Care, Substance Use, And Adherence To Therapy In Hiv/Aids, Patrice K. Nicholas, Suzanne Willard, Clinton J. Thompson, Carol Dawson-Rose, Inge B. Corless, +20 Additional Authors

GW Biostatistics Center

Engagement with care for those living with HIV is aimed at establishing a strong relationship between patients and their health care provider and is often associated with greater adherence to therapy and treatment (Flickinger, Saha, Moore, and Beach, 2013). Substance use behaviors are linked with lower rates of engagement with care and medication adherence (Horvath, Carrico, Simoni, Boyer, Amico, and Petroli, 2013). This study is a secondary data analysis using a cross-sectional design from a larger randomized controlled trial (n = 775) that investigated the efficacy of a self-care symptom management manual for participants living with HIV. Participants were …

Hiv Testing Implementation In Two Urban Cities: Practice, Policy, And Perceived Barriers., Camden J Hallmark, Jennifer Skillicorn, Thomas P Giordano, Jessica A Davila, Marlene Mcneese, Nestor Rocha, Avemaria Smith, Stacey Cooper, Amanda D. Castel

Epidemiology Faculty Publications

BACKGROUND: Although funding has supported the scale up of routine, opt-out HIV testing in the US, variance in implementation mechanisms and barriers in high-burden jurisdictions remains unknown.

METHODS: We conducted a survey of health care organizations in Washington, DC and Houston/Harris County to determine number of HIV tests completed in 2011, policy and practices associated with HIV testing, funding mechanisms, and reported barriers to testing in each jurisdiction and to compare results between jurisdictions.

RESULTS: In 2012, 43 Houston and 35 DC HIV-testing organizations participated in the survey. Participants represented 85% of Department of Health-supported testers in DC and 90% …

New Carbohydrate-Based Anti-Cancer And Anti-Bacterial Vaccines, Zhifang Zhou

Wayne State University Dissertations

The unique carbohydrates expressed on the surface of cancer, bacterial, viral and fungal cells are excellent target antigens for the design of therapeutic or preventive vaccines. However, as antigens carbohydrates have problems. First, carbohydrates usually have low immunogenicity. Second, even if immunogenic, carbohydrates typically elicit T cell-independent immune responses. To overcome these problems and design useful vaccines based on carbohydrate antigens, they are usually coupled with carrier proteins to form conjugates to enhance the immunogenicity of the antigens. However, there are still some issues existing in glycoprotein vaccines, such as poor reproducibility of the conjugates, difficulties in quality control and …

The Effects That Liquid And Solid Cattle Manure Have On The Water Quality Of Drainage Ditches In Putnam County, Ohio, Janelle Horstman

Honors Projects

Lake Erie has experienced harmful algal blooms with increased frequency since the mid-1990s due to excess nutrients from Rivers, such as the Maumee River, and largely agricultural watersheds. Nonpoint source pollution from agriculture contributes to eutrophication, algal blooms, and the degradation of water quality. This creates stress on aquatic fauna, reduced aesthetic quality, odor, and limits of the water for usage of drinking, recreation, and industry. This research paper asks what the contributions of having access to manure application records, soil records, and information about antibiotics have on what is known about manure management and antibiotic resistance, which has been …

Lost In Translation: Statistical Inference In Court, Erica Beecher-Monas

Law Faculty Research Publications

No abstract provided.

High-Level Expression Of The Brugia Malayi Protein Kinase, Bm-Mpk 1, In Insect Sf9 Cells, Arti Rana

Theses, Dissertations and Culminating Projects

Lymphatic Filariasis (elephantiasis) is a neglected tropical disease caused by the filarial nematodes Brugia malayi (B. malayi), Brugia timori, and Wuchererici Bancrofti (1,8). These parasites are present in over 83 counties in the tropics and sub-tropics where more than 1.4 billion people are at risk of infection and 130 million people are presently infected (1,7). Previous work from our lab has led to the identification and expression of a B. malayi stress-activated protein kinase, Bm-MPKl (a human p38/C. elegans PMK-1 ortholog). Bm-MPKl plays an important role in the parasites’ protection against oxidative stress (1), and as such is a potential …

Improving The Microbial Source Tracking Toolbox : Evaluating The Use Of Selective Enrichments And Artificial Sweeteners In A Multi-Tiered Approach, Stacey A. Helming

Legacy Theses & Dissertations (2009 - 2024)

Microbial contamination of surface waters is a public health concern world-wide, with non-point source fecal input contributing to millions of cases of waterborne illness annually. Numerous techniques have been proposed to monitor for non-point source fecal contamination, but few studies have explored the correlation of multiple chemical and microbial fecal source tracking markers in ambient waters. Therefore, the purpose of these studies was to evaluate the use of multiple "ideal" markers of fecal contamination and improve upon any analytical techniques required to identify species-specific fecal contamination in surface waters. The use of selective enrichments to improve the sensitivity of end-point …

Changing The Climate Narrative: How A Long-Term Climate Change Might Save Our Lives, Natalie P. Harreld

CMC Senior Theses

The goal of this paper is to offer new insights into the climate change debate by shifting away from the heated anthropologic arguments that dominate politics, media, and popular science. Instead, I choose to rely on the long-term impacts of a changing climate on our planet. The paper begins with a break down of key processes involved in short-term and long-term climate change, using the latest research. After a foundational understanding of climate sciences is established, we will discuss the failure of the climate change debate in educating the general public about the facts of a changing climate. Finally, the …

A Kinetic Analysis Of The Inhibition Of Fox-4 Β-Lactamase, A Plasmid-Mediated Ampc Cephalosporinase, By Monocyclic Β-Lactams And Carbapenems, Krisztina M. Papp-Wallace, Susana Mallo, Christopher R. Bethel, Magdalena A. Taracila, Andrea M. Hujer, Ana Fernandez, Julian A. Gatta, Kerri M. Smith, Yan Xu, Malcolm G.P. Page, Eric Desarbre, German Bou, Robert A. Bonomo

Chemistry Faculty Publications

Abstract: Objectives: Class C β-lactamases are prevalent among Enterobacteriaceae; however, these enzymes are resistant to inactivation by commercially available β-lactamase inhibitors. In order to find novel scaffolds to inhibit class C β-lactamases, the comparative efficacy of monocyclic β-lactam antibiotics (aztreonam and the siderophore monosulfactam BAL30072), the bridged monobactam β-lactamase inhibitor BAL29880, and carbapenems (imipenem, meropenem, doripenem and ertapenem) were tested in kinetic assays against FOX-4, a plasmid-mediated class C β-lactamase (pmAmpC). Methods: The FOX-4 β-lactamase was purified. Steady-state kinetics, electrospray ionization mass spectrometry (ESI-MS) and ultraviolet difference (UVD) spectroscopy were conducted using the β-lactam scaffolds described. Results: The Ki values …

Novel Tuberculostatic Agents Suitable For Treatment Of Mycobacterium Tuberculosis Infections Of The Central Nervous System, Ronald Bartzatt, Preeti Sule, Suat L.G. Cirillo, Jeffrey D. Cirillo

Chemistry Faculty Publications

Aims: To demonstrate the efficacy of five small molecule compounds for inhibiting the growth of Mycobacterium tuberculosis. To present evidence that these compounds will penetrate into the central nervous system.

Study Design: Five small molecule compounds bearing a hydrazide group were synthesized utilizing microwave excitation. These compounds were then placed into tissue culture with Mycobacterium tuberculosis at various concentrations for evaluation of bacterial growth inhibition.

Place and Duration of Study: The compounds to be tested were prepared at the University of Nebraska Chemistry Department August 2013. The evaluation of antibacterial activity was determined at the Texas A&M Health Science …

Improving Human Health By Increasing Access To Natural Areas: Opportunities And Risks, Bradford S. Gentry, David Krause, Karen A. Tuddenham, Sarah Barbo, Benjamin D. Rothfuss, Christopher Rooks

Yale School of the Environment Publications Series

Report of the 2013 Berkley Workshop

Held at the Pocantico Center of the Rockefeller Brothers Fund, Tarrytown, NY - July 2013

Indemics: An Interactive High-Performance Computing Framework For Data Intensive Epidemic Modeling, Keith R. Bisset, Jiangzhuo Chen, Suruchi Deodhar, Xizhou Feng, Yifei Ma, Madhav V. Marathe

Mathematics, Statistics and Computer Science Faculty Research and Publications

We describe the design and prototype implementation of Indemics (_Interactive; Epi_demic; _Simulation;)—a modeling environment utilizing high-performance computing technologies for supporting complex epidemic simulations. Indemics can support policy analysts and epidemiologists interested in planning and control of pandemics. Indemics goes beyond traditional epidemic simulations by providing a simple and powerful way to represent and analyze policy-based as well as individual-based adaptive interventions. Users can also stop the simulation at any point, assess the state of the simulated system, and add additional interventions. Indemics is available to end-users via a web-based interface.

Detailed performance analysis shows that Indemics greatly enhances the …

Teaching Climate Literacy Using Geospatial Tools, Steven L. Babcock

LSU Master's Theses

Antarctica is the world’s coldest, driest and windiest continent. It is a harsh environment that few people will ever see but it is a very important part of our Earth system. Over the past 34 million years the climate in Antarctica has deteriorated from one that supported lush vegetation to the conditions observed today. By studying this trend and the associated changes to ice and vegetation we can gain critical insight into climate changes taking place today. This thesis presents three pieces of curricula that will help students and the general public understand some of the research currently underway in …

Semantics In Support Of Biodiversity Knowledge Discovery: An Introduction To The Biological Collections Ontology And Related Ontologies, Ramona L. Walls, John Deck, Robert Guralnick, Steve Baskauf, Reed Beaman, Stanley Blum, Shaun Bowers

Computer Science Faculty Scholarship

The study of biodiversity spans many disciplines and includes data pertaining to species distributions and abundances, genetic sequences, trait measurements, and ecological niches, complemented by information on collection and measurement protocols. A review of the current landscape of metadata standards and ontologies in biodiversity science suggests that existing standards such as the Darwin Core terminology are inadequate for describing biodiversity data in a semantically meaningful and computationally useful way. Existing ontologies, such as the Gene Ontology and others in the Open Biological and Biomedical Ontologies (OBO) Foundry library, provide a semantic structure but lack many of the necessary terms to …

Exposure Of Feral Swine (Sus Scrofa) In The United States To Selected Pathogens, John A. Baroch, Carl A. Gagnon, Sonia Lacouture, Marcelo Gottschalk

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine (Sus scrofa) are widely distributed in the United States. In 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the US to evaluate exposure to important swine endemic pathogens. Antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) were found in 2.5% and 25.3% of tested sera, respectively. Positive serological reactions against Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae have been detected in 19.7% and 69.7% of animals. More than 15% of animals presented antibodies against these 2 pathogens simultaneously. Most animals were also seropositive for …

Using Structural Analysis To Investigate The Function Of Suppressor Of Ikk-Epsilon (Sike), Sean W. Mckinley

Theses and Dissertations

The innate immune system provides the body’s first line of defense against pathogenic challenge through pathogen recognition and initiation of the immune response. Among the various cellular mechanisms of pathogen recognition in mammals, Toll-like receptor 3 (TLR3) recognizes viral dsRNA. Stimulation of TLR3 signaling pathway leads to transcription of pro-inflammatory cytokines and type-1 Interferons. Suppressor of IKKε (SIKE) interacts with two kinases in the signaling pathway, IKKε and TANK binding kinase 1 (TBK1), inhibiting the transcription of type I interferons. Recently, the Bell Laboratory discovered that SIKE blocks TBK1-mediated activation of type I interferons by acting as a high affinity, …

Finding Them Before They Find Us: Informatics, Parasites, And Environments In Accelerating Climate Change, Daniel R. Brooks, Eric P. Hoberg, Walter A. Boeger, Scott Lyell Gardner, Kurt E. Galbreath, David Herczeg, Hugo H. Mejía-Madrid, S. Elizabeth Rácz, Altangerel Tsogtsaikhan Dursahinhan

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Parasites are agents of disease in humans, livestock, crops, and wildlife and are powerful representations of the ecological and historical context of the diseases they cause. Recognizing a nexus of professional opportunities and global public need, we gathered at the Cedar Point Biological Station of the University of Nebraska in September 2012 to formulate a cooperative and broad platform for providing essential information about the evolution, ecology, and epidemiology of parasites across host groups, parasite groups, geographical regions, and ecosystem types. A general protocol, documentation–assessment–monitoring–action (DAMA), suggests an integrated proposal to build a proactive capacity to understand, anticipate, and respond …