Digital Commons Network^™

Antibiotic Resistance In A Coastal River In Mississippi, Usa – Potential Drivers, Shuo Shen, Wei Wu, Eric A. Saillant, Darrell Jay Grimes

Gulf and Caribbean Research

Wastewater treatment plants (WWTPs) are major sources of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in water bodies. Most studies on the impact of WWTPs on antibiotic resistance have focused on freshwater systems, with little information on coastal and estuarine waters with variable salinity. This study monitored seasonal levels of ARGs at the effluent and downstream of the Pascagoula— Moss Point WWTP in the lower Pascagoula River, a coastal river in southeastern Mississippi, USA. Surface water samples were collected seasonally at upstream, outflow, and 3 downstream sites from February to November 2016. Bacterial resistance to sulfamethazine, tetracycline, and …

Synthesis Of Cu-Cy Nanoparticles For Treating Pfas In Water, Ligang Wang

Physics Theses - Archive

This work aims to address the cumulative harm caused by perfluoroalkyl and polyfluoroalkyl substances (PFAS) in water to human health and the ecological environment around the world. Reducing or removing PFAS pollution is an urgent need. Cu-Cy nanoparticles are photosensitizers, which were developed for photodynamic therapy of cancer in the first place. In this work, we use Cu-Cy for the catalytic degradation of PFAS. Synthesis of Cu-Cy nanoparticles in deionized water at 90 degrees Celsius is a low-cost and efficient method. We demonstrated the detailed process of synthesizing Cu-Cy nanoparticles. Under ultraviolet illumination, the synthesized Cu-Cy nanoparticles turned yellow as …

When Brain Meets Artificial Intelligence, Lu Zhang

Computer Science and Engineering Dissertations - Archive

When we review the history of development of artificial intelligence (AI), we will find that brain science plays a pivotal role in fostering breakthroughs in AI, such as artificial neural networks (ANNs). Today, AI has made remarkable strides, particularly with the emergence of large language models (LLMs), surpassing expectations and achieving human-level performance in certain tasks. Nonetheless, an insurmountable gap remains between AI and human intelligence. It is urgent to establish a bridge between brain science and AI, promoting their mutual enhancement and collaborations. This involve establishing connections from brain science to AI (brain-inspired AI), and reversely, from AI to …

New Methods In Electrical Source Imaging Based On Eeg And Post-Mortem Pathology Data, Julio Cesar Enciso Alva

Mathematics Dissertations - Archive

A central task for Neuroscience is to determine the location of electrical activity of neural origin inside the brain. Electrical signals can be recorded at a high resolution in time but low resolution in space, thus making it difficult to locate their source unambiguously. Electrical Source Imaging (ESI) is a particular framework for neural electrical source location; it is possible by modeling any additional information we may have about the electrical sources. For instance, minimal-norm estimators assume that the most plausible estimation is that with a lower norm. However, these estimators possess a low resolution in space.

In this work, …

Genetic Tracking Of A Rabid Coyote (Canis Latrans) Detected Beyond A Rabies Enzootic Area In West Virginia, Usa, Matthew W. Hopken, Crystal Gigante, Amy T. Gilbert, Richard B. Chipman, Jordana D. Kirby, Rene Edgar Condori, Samuel Mills, Chelsea Hartley, John Forbes, Lisa Dettinger, Dongxiang Xia, Yu Li, Bridgett Vonholdt

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife translocation and cross-species transmission can impede control and elimination of emerging zoonotic diseases. Tracking the geographic origin of both host and virus (i.e., translocation versus local infection) may help determine the most effective response when high-risk cases of emerging pathogens are identified in wildlife. In May 2022, a coyote (Canis latrans) infected with the raccoon (Procyon lotor) rabies virus variant (RRV) was collected in Lewis County, West Virginia, USA, an area free from RRV. We applied host population genomics and RRV phylogenetic analyses to determine the most likely geographic origin of the rabid coyote. Coyote …

Branching Fractional Brownian Motion, Reece Beattie-Hauser

Masters Theses

"Fractional Brownian Motion (FBM) is a Gaussian process whose increments are correlated over long times. FBM is an example of anomalous diffusion, and recently it has been used to model the distribution of serotonergic fibers in the brain [1, 2]. To better represent these fibers, branching FBM (bFBM), where FBM trajectories may randomly split into two, is introduced. One-dimensional bFBM is studied in both sub diffusive and super diffusive regimes, examining three potential behaviors of the correlations (memory) in a branching event: both trajectories retain the memory of previous steps, only one keeps the memory, and neither keeps the memory. …

Embracing Ai In English Composition: Insights And Innovations In Hybrid Pedagogical Practices, James Hutson, Daniel Plate, Kadence Berry

Faculty Scholarship

In the rapidly evolving landscape of English composition education, the integration of AI writing tools like ChatGPT and Claude 2.0 has marked a significant shift in pedagogical practices. A mixed-method study conducted in Fall 2023 across three sections, including one English Composition I and two English Composition II courses, provides insightful revelations. The study, comprising 28 student respondents, delved into the impact of AI tools through surveys, analysis of writing artifacts, and a best practices guide developed by an honors student. Initially, the study observed a notable anxiety and mistrust among students regarding the use of AI in writing. However, …

Chemical Control Of Turfgrass Diseases 2024, Bruce B. Clarke, Paul Vincelli, Paul Koch, Ming-Yi Chou

Agriculture and Natural Resources Publications

Turfgrasses under intensive management are often subject to outbreaks of infectious diseases. Diseases usually are most damaging when weather or cultural conditions favor the disease-causing agent but not plant growth and vigor. Cultural conditions that predispose turfgrass to diseases include close mowing, inadequate or excessive fertility, light or frequent irrigation, excessive thatch, poor drainage, and shade. Good turf management practices often greatly reduce the impact of disease by promoting healthy plants that are better able to resist infections. Even under good management, however, diseases sometimes cause excessive damage to highly managed turfgrasses. The proper use of fungicides in these instances, …

Exposure To Metal Accumulation In Chicken Giblets And Human Health Risk In Lokoja, Nigeria, Jude Ehwevwerhere Emurotu, Lami Habib, Ephraim Musa Dallatu, Adamu Shuaibu Onakpa, Aloysius Akaangee Pam

Applied Environmental Research

When consumed, the toxic heavy metals in chicken giblets pose a grave risk to humans. An inductively coupled plasma optical emission spectrometer (ICP-OES) was used to measure the concentrations of metals (Al, Cd, Co, Cr, Ni, Mn, and Pb) in the liver, gizzard, and kidney of chickens from the Lokoja poultry market. Except for lead, which slightly exceeded the FAO/WHO limit of 0.1 mg kg-1, the metal concentrations found were all within the threshold limit. The estimated daily intake (EDI), hazard quotient (HQ), hazard index (HI), and cancer risk (CR) were used to assess the risks that humans would face …

Minimization The Chemical Oxygen Demand (Cod) Content In Tannery Wastewater Using Activated Carbon From Spent Tea Leaves, Kanish Fatama, Nasifa Akter, Badhan Saha, Nur -E- Alam Md., Shimul Chakma

Applied Environmental Research

Water pollution from tannery wastewater is a serious environmental issue. This study aimed to investigate the potential of spent tea leaves as activated carbon (AC) to minimize chemical oxygen demand (COD) of tannery effluent. To determine the best conditions for minimizing COD, a batch adsorption process was conducted, involving various factors such as different adsorbent dosages (ranging from 1 to 11 gm L-1), contact time (30 to150 min), and pH values (ranging from 3 to 11). The characterization was done by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM). The outcomes of this study demonstrated that using 1.5 M …

Outlooks And Challenges For Urban Green Space Development: A Review Case Study In Thailand, Kanawat Paoin, Chanathip Pharino

Applied Environmental Research

Even though prior studies have provided ample proof of the benefits of urban green space, Thailand's development and exploitation of urban green space remain insufficiently efficient. To assess the prospects for urban green space development in Thailand, our review study highlighted gaps and challenges related to these areas. This review study discussed the rationale for urban green space, its definitions, as well as its benefits and co-benefits (i.e., economic, social, health, and environmental). The review research additionally discussed Thailand's current urban green space issues, associated challenges (such as difficulties valuing and using urban green space, budgetary limitations, low priority for …

Global Perspectives On Healthcare Waste Management And Bibliographic Mapping During The Crises Of Covid-19, Vasudeo Zambare, Bhushan Bhusare, Lew Christopher, Mohanadoss Ponraj, Nabin Narayan Munankarmi, Mohd Fadhil Md. Din

Applied Environmental Research

The lack of proper waste management of health care facilities can have a significant negative health impact on the global population. This study provides a critical view on the current state of the existing healthcare waste management practices used in healthcare facilities around the world during the Coronavirus disease 2019 (COVID-19) pandemic outbreak. The increasing amount of COVID-related healthcare waste was analyzed and classified to provide justification for the urgent need to develop waste management standards that ensure safe containment and diversion of hazardous and non-hazardous wastes through recycling, reuse and repurposing. The waste handling, treatment, and disposal management of …

Assessment Of Total Petroleum Carbon And Heavy Metals Of Some Selected Plants Grown In Crude Oil Polluted Soils Using Gc-Fid, Pius O. Adah, Ndem E. Edu, Reagan B. Agbor, Henry B. Kogbara

Applied Environmental Research

This study was focused on assessing the phytoremediation potentials of some selected plant species on crude oil contaminated soils. Five plant species were cultivated in soils polluted with crude oil at 10, 50, 100, 150 and 200 mg kg-1. The results revealed that there were significant reduction in THC contents of soil ameliorated with the various plants species compared to the control group increases with increasing concentration of crude oil pollutant in soil. THC was 62.24 mg kg-1, 1,120 mg kg-1, 208.67 mg kg-1, 257.87 mg kg-1 and 2,762.79 mg kg-1 for R. simplex, P. balfouriana, K. pinnata …

Food Waste From Catering Services In Thai Hotels: A Composition Analysis And Practical Guidelines For Effective Utilization, Patranit Srijuntrapun, Patinya Sukwong

Applied Environmental Research

Food waste from the hotel sector is a significant contributor to the overall volume of food waste, particularly in restaurants and food services through various processes. However, precise data on food waste generated by the hotel industry is lacking. This study aims to quantify and characterize the food waste generated during the preparation and serving processes in hotel catering, with the goal of finding efficient solutions to reduce food waste that are appropriate for the hotel and catering sectors. The study presents novel findings that among the different types of catering, dinner-style buffets (DB) generate the highest amount of food …

Determinants Of Physicochemical Composition Of Palm Oil Mill Effluent - Implications On Environment And Bio-Digester Treatment Design, E.B. Tambe, A.U. Okonkwo, I.E. Mbuka-Nwosu, C.O. Cookey, E. Agbe, S.C. Onwusa, I.N. Ekpe, U.F. Evuen, J. Okpoghono

Applied Environmental Research

Growing need for renewable energy and addressing challenges associated with indiscriminate wastes disposal has necessitated harnessing wastes to wealth. The study investigated parameters determining the physicochemical composition of palm oil mill effluent (POME) generated at Agricultural Development Authority Palm (ADAPALM) and palm oil mills in its catchment communities, located at Ohaji/ Egbema Local Government Area of Imo State, Nigeria and their implications on environmental health and treatment approaches required. Survey research design was used. From a randomly sampled small-scaled mill in each community, and for the lone medium and large-scaled mills, four homogenous samples of POME were collected from sampled …

Removal Of Chromium From Aqueous System Using Nanocellulose Prepared From Defoliated Terminalia Catappa Leaves, Lopamudra Digal, Jayashree Mohanty

Applied Environmental Research

Nanocellulose is considered as a sustainable and renewable nanomaterial due to its abundant availability, biodegradability and biocompatibility characteristics. The study demonstrates the synthesis of nanocellulose from defoliated leaves of the Tropical Almond (Terminalia catappa) plant, abundantly available residual plant biomass and its subsequent use in eliminating Cr(VI) from the water system. The study demonstrates the preparation of nanocellulose by chemical means, employing NaOH and H2SO4 as chemical agents. Characterization techniques like FTIR, SEM, and XRD were used to study the chemical composition and morphology of the resulting nanocellulose. The characterization studies confirm the formation of nanocellulose from the plant biomass. …

Monitoring Of Estrone From Hospital Wastewater And Its Removal By Photocatalytic Membrane Reactor, Nicha Karnjanamit, Chongdee Thammakhet-Buranachai, Panitan Jutaporn, Watsa Khongnakorn

Applied Environmental Research

Steroid hormones, classified as endocrine-disrupting compounds (EDCs), pose significant environmental and health concerns due to their persistence and potential adverse effects. This study investigates the presence of estrone (E1) in hospital wastewater and the efficiency of E1 removal through a photocatalytic membrane reactor (PMR). Wastewater samples were collected from two hospitals with different wastewater treatment processes. The concentration of E1 was analyzed using a combination of solid phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The PMR experimental setup involved a polyvinylidene fluoride (PVDF) membrane incorporated with 1 wt% titanium dioxide (PVDF/TiO2). The PMR performance was evaluated with and …

Unraveling The Effects Of Arbuscular Mycorrhizal Fungi On Plant Growth, Nutrient Content, And Heavy Metal Accumulation In The Contaminated Soil: A Meta-Analysis, Bela Putra, Anuraga Jayanegara, Irwan Susanto

Applied Environmental Research

This study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on plant growth, nutrient uptake, and heavy metal accumulation on polluted land using a meta-analysis approach. Data from 33 relevant studies were selected based on inclusion criteria, specifically articles in English, observational research, and investigating the role of AMF in plant growth and productivity on polluted land. The results showed that plants inoculated with AMF experienced significant accumulation of heavy metals in roots, such as Pb (p<0.01), Ni (p<0.01), Cr (p<0.01), Mn (p<0.05), Fe (p<0.05), and As (p<0.05). The AMF significantly reduced the accumulation of heavy metals such as Cr, Ni, Fe, and Cu on the upper part of fodder forage (p<0.01). Forage growth was also enhanced due to AMF. The AMF greatly increased the fresh weight, length, and phosphorus (P) content of fodder forage roots (p<0.01). It also increased the plant's biomass, fresh weight, dry weight, height, nitrogen (N), phosphorus (P), and potassium (K) contents (p<0.01). In conclusion, AMF is important in increasing plant growth, nutrient uptake and reducing heavy metal accumulation in forage on polluted land.

Numerical Dose Assessment And Short-Term Radioactivity Impact On Foodstuff For Continuous Release From Of Triga Mark Ii Research Reactor, Ahmed Dahia, Amel Dadda, Amina Lyria Cheridi Deghal, Abdellah Bouam

Applied Environmental Research

This work is a contribution to the assessment of the radiological consequences of radioactivity and radiation dose for TRIGA Mark II research reactor during continuous operation. The potential release to the atmosphere of 131I, 137Cs, and 90Sr are computed in the South direction is calculated using CROM software. We have attempted to evaluate the daily concentration of radioactivity and its impact on foodstuff. The annual average dose received from internal and external irradiation by age group through the different pathways were also considered. The simulation results showed that the highest air concentration was found at 225 m distance from the …

Synthesis And Evaluation Of 5-(Heteroarylmethylene)Hydantoins As Glycogen Synthase Kinase-3Β Inhibitors, Nicholas O. Schneider, Keandra Gilreath, Daniel J. Burkett, Martin St. Maurice, William A. Donaldson

Chemistry Faculty Research and Publications

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase which plays a center role in the phosphorylation of a wide variety of proteins, generally leading to their inactivation. As such, GSK-3 is viewed as a therapeutic target. An ever-increasing number of small organic molecule inhibitors of GSK-3 have been reported. Phenylmethylene hydantoins are known to exhibit a wide range of inhibitory activities including for GSK-3β. A family of fourteen 2-heterocycle substituted methylene hydantoins (14, 17–29) were prepared and evaluated for the inhibition of GSK-3β at 25 μM. The IC50 values of five of these compounds was determined; the two best inhibitors …

Disentangling Cyclic Causality: An Instance-Based Framework For Causal Discovery, Chase A. Yakaboski

Dartmouth College Ph.D Dissertations

Correlation does not imply causation" is one of the fundamental principles taught in science, emphasizing that associations between variables do not necessarily indicate causality. Yet, over the past three decades, extensive research has begun to challenge this perspective by developing sophisticated methods to differentiate causal from correlative relationships. This research suggests that correlations often involve a blend of confounded and causal interactions, which, given certain assumptions, can be disentangled to uncover actionable insights and deepen our understanding of physical, biological, and societal systems.

Accurately discovering causal relationships from data amidst cyclic dynamics remains a challenging open problem in causality research. …

Scalable Methods For Resource-Constrained Adaptive Sampling, Kizito Masaba

Dartmouth College Ph.D Dissertations

As the global community confronts the pressing issues of climate change, the importance of environment monitoring cannot be overstated. This process is essential in identifying and tracking critical environmental trends to facilitate efficient and effective conservation efforts. Traditionally, scientists perform this task using hand-held, in-situ instruments. With the recent robot advancements, this task can be fully automated by applying multi-robot systems to a task referred to as multi-robot adaptive sampling. However, the cost of purchasing and operating robot systems for this application are still prohibitive due to various environmental, technological, and logistical constraints. Some of the outstanding constraints to the …

Modeling Apparent Pb Loss In Zircon U-Pb Geochronology, Glenn R. Sharman, Matthew A. Malkowski

Geosciences Faculty Publications and Presentations

The loss of radiogenic Pb from zircon is known to be a major factor that can cause inaccuracy in the U–Pb geochronological system; hence, there is a need to better characterize the distribution of Pb loss in natural samples. Treatment of zircon by chemical abrasion (CA) has become standard practice in isotope dilution–thermal ionization mass spectrometry (ID-TIMS), but CA is much less commonly employed prior to in situ analysis via laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) or secondary ionization mass spectrometry (SIMS). Differentiating the effects of low levels of Pb loss in Phanerozoic zircon with relatively low-precision in situ U–Pb …

Re–Operating The Bhumibol And Sirikit Dams Using Hybrid Neuro–Fuzzy Technique To Solve The Water Scarcity And Flooding Problems In The Chao Phraya River Basin, Khin Muyar Kyaw, Areeya Rittima, Yutthana Phankamolsil, Allan Sriratana Tabucanon, Wudhichart Sawangphol, Jidapa Kraisangka, Yutthana Talaluxmana, Varawoot Vudhivanich

Applied Environmental Research

The decision support system to reservoir re–operation using Artificial Intelligence has been broadly studied and proven in term of the operational performances for both single and multiple reservoir system, this study applied Adaptive Neuro Fuzzy Inference System (ANFIS) technique for reservoir re–operation in Chao Phraya River Basin aiming to reduce water scarcity and flooding problems in the central region of Thailand. ANFIS is an integrated approach in which neural networks are utilized to enhance the fuzzy inference system and create fuzzy “IF–Then” reservoir operational guidelines with proper membership functions for reservoir re–operation. In this study, ANFIS operating rules were trained …

Social Return On Investment (Sroi) From The Community Waste Bank Implementation Under The Learning Center Of Systematic Waste Management Innovation According To The Circular Economy Concept, Pornpod Sridan, Pichaya Surapolchai, Thidasil Plainlaor, Apinya Tanyaim, Nuchanad Hounnaklang, Onuma Zongram, Yotwadee Hawangchu

Applied Environmental Research

This article presents the results of social return on investment (SROI) evaluation in the community’s waste bank program implemented in the Moo 7 and Moo 8 Villages of Tan Diao Subdistrict, Kaeng Khoi District, Saraburi Province during 2020–2022, which is a part of the Learning Centre of Systematic Waste Management Innovation According to the Concept of Circular Economy project. This research employed participatory action research to collect data on total cost for investment and income from selling recyclable waste through in-depth interviews and focus groups with a total of 40 stakeholders including: 25 community members, 5 community leaders, 3 representatives …

Luminescence Inhibition Of Water-Soluble Fractions Of Crude Oil, Diesel And Spent Engine Oil On Freshly Isolated Vibrio Campbellii, Beatrice Omolola Bello, Olayide Samuel Lawal, Gbenga Emmanuel Olumayede, Ogochukwu Angela Agwu, Stanley Ozoemena Agbo

Applied Environmental Research

Inhibitory effects of water-soluble fraction (WSF) of crude oil, diesel, spent engine oil, and their composite mixture on luminescent Vibrio species isolated from a tropical marine fish were assessed to determine the acute toxicity and suitability of the bacteria. This was evaluated with a slight modification of the procedure for freshly prepared luminescent marine bacteria according to the International Organization for Standardization. Toxicity was assessed following bacterial exposure to different concentrations of the WSFs. Water quality parameters and total polycyclic aromatic hydrocarbons (PAHs) were determined using standard methods. Chemical analysis revealed cumulative loads of PAHs in the WSFs of crude …

The Influence Of Chemical Fertilizers On The Effectiveness Of Biochar In Mitigating Cadmium Mobility In Soil, Chompoonut Chaiyaraksa, Navapat Sangworn

Applied Environmental Research

The focus of this study was to explore how various fertilizers influence the movement of cadmium in soil treated with biochar. The research utilized a strong acid sandy loam soil from Chanthaburi Province, naturally rich in organic matter with moderate cation exchange capacity and low nitrogen, potassium, phosphorus, sulfate, salinity and chloride levels. The soil was purposely contaminated with 50 mg kg-1 of cadmium and treated with biochar derived from water hyacinth through pyrolysis at 450°C for an hour. This biochar displayed moderate alkalinity, high organic matter, phosphorus, potassium, and cation exchange capacity, but low nitrogen content. Analytical techniques like …

A Review On Chemometrics And Climate Change: Insights From Malaysia Case Study, Ismafatin Nabilah Ismail, Mohd Khairul Amri Kamarudin, Wan Nor Azilawanie Tun Ismail, Noorjima Abd Wahab, Ahmad Shakir Mohd Saudi, Mohd Ekhwan Toriman, Firdaus Mohamad Hamzah, Septi Ariadi

Applied Environmental Research

Climate change consider as main issues and concerns in Malaysia over the decade. Chemometrics techniques have been used in many climate change issues to address the limitations of climate change monitoring studies. In climate change areas, chemometrics is a valuable tool for assessing the correlation between different climate change factors, especially in vast and intricate databases. The purpose of the review was to evaluate and summarise the available data and restrictions regarding the use of chemometrics techniques in Malaysian environmental studies. The study conducted a thorough analysis of pertinent scientific papers published between 2019 to 2023 that addressed the main …

Optimizing Tenebrio Molitor Feeding Strategy To Induce Physical Degradation Of Tires: A Novel Approach For Sustainable Waste Management, Abdussalam Topandi, Khadijah Sayyidatun Nisa, Fatimah Azizah Riyadi, Ibnu Maulana Hidayatullah, Pranata -, Firah Gustianda Ayu, Reynaldi Ivan Prasetya, Frederick Soetandar, Dimas Rafi Narawangsa, Eka Rahayu

Applied Environmental Research

Due to the increased usage of tires, the accumulation of worn tires has become a significant environmental concern and necessitates environmentally friendly waste management strategies. Conventional methods, such as tire incineration or improper disposal, contribute to environmental degradation. It is reported that organisms, such as Tenebrio molitor larvae, can degrade a wide range of polymers. This study explores the promising potential of Tenebrio molitor larvae in addressing this concern. Through a series of experiments, Tenebrio molitor larvae were fed with cut-worn tires to facilitate degradation. The larvae exhibited remarkable capabilities, surviving and thriving on tires as a sole carbon source …

Fretting Corrosion Wear Of Titanium-Based Alloy (Ti-6al-4v) In 0.9 Wt.% Nacl Solution, Chen-En Lu, Wen-Ken Li, Hung-Hua Sheu, Jeou-Long Lee, Hung-Bin Lee

Journal of Marine Science and Technology–Taiwan

In this study, a high precision fretting tribo-corrosion tester used to analyze the wear and corrosion behavior of Ti alloys in a fretting motion condition. The tester, operated in a ball-on-plate mode, was employed in the fretting corrosion study of metallic bio-materials (Ti and Ti-6Al-4V) under different loads (0 and 10 N), electrolytes (0.9 wt% NaCl and SBF) and fixed displacement frequency (1 Hz). Using profilometer, optical microscope in the experimental analysis and three-body mechanism in the theoretical analysis, the fretting corrosion performances of these bio-medical metals, such as velocity accommodation characteristics, friction, wear, and scratch profile were examined in …