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Insulin Based Inhibitors Of Human Islet Amyloid Polypeptide (Hiapp) And Their Effect On Aggregation Of Hiapp In The Treatment Of Type Ii Diabetes, Ranadheer R. Pesaru

Master's Theses and Doctoral Dissertations

Human islet amyloid polypeptide protein (hIAPP) is secreted by the pancreas along with insulin and is assumed to play a role in pathological development of type II diabetes. It has 37 amino acids in its sequence. Amyloid is formed due to misfolding of the protein, which is cytotoxic to beta cells in the pancreas of type II diabetic patients. The presence of amyloid deposits is also a characteristic feature of a number of other diseases like Alzheimer’s disease and Parkinson’s disease. Since insulin has been reported to interact with hIAPP and block amyloid formation, fragments of insulin were synthesized and …

Racial Disparities Study In Diabetes-Related Complication Using National Health Survey Data, Fengxia Yan

Mathematics Theses

The main aim of this study is to compare the prevalence of diabetes-related complications in white to the prevalence in other racial and ethnic groups in United States using 2009 Behavioral Risk Factor Surveillance System (BRFSS). By constructing the logistic regression model, odds ratios (OR) were calculated to compare the prevalence of diabetes complications in white and other groups. Compared to white, the prevalence of hypertension and stroke in African Americans were higher, while the prevalence of heart attack and coronary heart disease were lower. The Asian Americans or Pacific Islanders, African Americans and Hispanics were more likely to develop …

Chromatographic Studies Of Drug-Protein Binding In Diabetes, Kathryn (Krina) S. Joseph

Department of Chemistry: Dissertations, Theses, and Student Research

Drug-protein binding can have a dramatic impact on the distribution and metabolism of a drug. This manuscript describes the use of high-performance affinity chromatography to examine the binding of various compounds to human serum albumin (HSA) in normal and diabetic disease states.

The first study examined the use of four coumarin compounds as possible alternatives to warfarin as a probe for Sudlow site I on HSA. High-performance affinity chromatography and immobilized HSA columns were used to compare and evaluate the binding properties of these probe candidates. It was found from this group that 4-hydroxycoumarin was the best alternative to warfarin …

Trust And Trustworthiness: A Framework For Successful Design Of Telemedicine, James Robert Templeton

CCE Theses and Dissertations

Trust and its antecedents have been demonstrated as a barrier to the successful adoption of numerous fields of technology, most notably e-commerce, and may be a key factor in the lack of adoption or adaptation in the field of telemedicine. In the medical arena, trust is often formed through the relationships cultivated over time via clinician and patient. Trust and interpersonal relationships may also play a significant role in the adoption of telemedicine. The idea of telemedicine has been explored for nearly 30 years in one form or another. Yet, despite grandiose promises of how it will someday significantly improve …

Methods Of Competing Risks Analysis Of End-Stage Renal Disease And Mortality Among People With Diabetes, Hyun J. Lim, Xu Zhang, Roland Dyck, Nathaniel Osgood

Mathematics and Statistics Faculty Publications

Background: When a patient experiences an event other than the one of interest in the study, usually the probability of experiencing the event of interest is altered. By contrast, disease-free survival time analysis by standard methods, such as the Kaplan-Meier method and the standard Cox model, does not distinguish different causes in the presence of competing risks. Alternative approaches use the cumulative incidence estimator by the Cox models on cause-specific and on subdistribution hazards models. We applied cause-specific and subdistribution hazards models to a diabetes dataset with two competing risks (end-stage renal disease (ESRD) or death without ESRD) to measure …

Succination Of Thiol Groups In Adipose Tissue Proteins In Diabetes: Succination Inhibits Polymerization And Secretion Of Adiponectin, Norma Frizzell, Mathur Rajesh, Matthew J. Jepson, Ryoji Nagai, James A. Carson, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

S-(2-Succinyl)cysteine (2SC) is formed by reaction of the Krebs cycle intermediate fumarate with cysteine residues in protein, a process termed succination of protein. Both fumarate and succination of proteins are increased in adipocytes cultured in high glucose medium (Nagai, R., Brock, J. W., Blatnik, M., Baatz, J. E., Bethard, J., Walla, M. D., Thorpe, S. R., Baynes, J. W., and Frizzell, N. (2007) J. Biol. Chem. 282, 34219-34228). We show here that succination of protein is also increased in epididymal, mesenteric, and subcutaneous adipose tissue of diabetic (db/db) mice and that adiponectin is a major target for succination in both …

Cardiac And Renal Function Are Progressively Impaired With Aging In Zucker Diabetic Fatty Type Ii Diabetic Rats, John W. Baynes, David B. Murray

Faculty Publications

This study investigated the temporal relationship between cardiomyopathy and renal pathology in the type II diabetic Zucker diabetic fatty (ZDF) rat. We hypothesized that changes in renal function will precede the development of cardiac dysfunction in the ZDF rat. Animals (10 weeks old) were divided into four experimental groups: Lean Control (fa/?) LC(n = 7), untreated ZDF rats (n = 7) sacrificed at 16 weeks of age, and LC (n = 7) untreated ZDF rats (n = 9) sacrificed at 36 weeks of age. LV structural/functional parameters were assessed via Millar conductance catheter. Renal function was evaluated via markers of …

A Preliminary Study Of A Non-Invasive Glucose Sensor Based On A Mercury Sensor, Erin Wood

Electronic Theses and Dissertations

Diabetes mellitus is a potentially lethal disease that affects 7.6 percent of American people. In the US, it is recognized as the 6th leading cause of death. Failure to control blood glucose levels (BGL) in patients with either type of diabetes can lead to other serious complications as well, such as loss of limb, blindness and other health problems. Controlling and monitoring the BGL in post-op and intensive care patients in the hospital is also vital to their health. Currently the most reliable method of monitoring BGL is through an invasive procedure which monitors the amount of glucose in blood …

Self-Management Of Type 2 Diabetes In Appalachina Women., Melissa Joy Magness

Electronic Theses and Dissertations

Gender, minority, and regional-related disparities have been documented in diabetes management. Self-efficacy, the belief in one's ability to carry out the actions mandated by a task, has been identified as a key predictor in glycemic control; however, it has not been investigated in rural, female populations. This cross-sectional, correlation investigation examined the relationships among self-efficacy, depression, and diabetes self-care management in women living in Appalachia with type 2 diabetes. Using Bandura's Social Cognitive Theory, 85 women ages ≥ 21 with type 2 diabetes for a minimum of 6 months who were residents in Appalachia completed the 1) Diabetes Self-Efficacy Scale, …

Jonas Kamlet (1914-1960) Chemist/Entrepreneur, Dean F. Martin, Barbara B. Martin

Chemistry Faculty Publications

Jonas Kamlet, Ph.D., as a chemist, inventor, and a significant entrepreneur who founded Kamlet Laboratories in New York City that became the model of a successful consulting firm. Among his hundreds of patents and inventions were a tablet that could be used to measure glucose in urine, a treated strip that could detect pregnancy, a fodder for ruminants that used newsprint, and biuret (a self-condensation product of urea) that could be used as a non-protein component of fodder. Following his death in an airplane collision on December 16, 1960, his wife and collaborator (and later a resident of Florida) carried …

Predictors Of Autonomy Support At Diabetes Summer Camp: A Self-Determination Theory Approach, Ron Ramsing, Jim Sibthorp

Research in Outdoor Education

Diabetes is considered to be one of the most psychologically and behaviorally demanding chronic illnesses facing adolescents (Cox & Gonder-Fredrick, 1992). To control the disease more effectively, adolescents must learn to undertake the management of their diabetes themselves (Mensing, et al., 2000; Ruggiero, et al., 1997). Self-determination theory (Ryan & Deci, 2000) was used as a foundation to explore the mechanisms, within a summer camp, that led to increased perceptions of autonomy support. Research shows autonomy support to be a critical prerequisite for increased autonomous behavior.

With over 350 specialty camps serving youth with diabetes (Diabetes Education and Camping Association, …

Slides: Survey Of Indian Groundwater Issues, Rodney B. Lewis

Groundwater in the West (Summer Conference, June 16-18)

Presenter: Rodney Lewis, General Counsel, Gila River Tribe.

49 slides.

The Effects Of Intentional Recreation Programming On Internalization Of Type 1 Diabetes Management Among Adolescents, Eddie Hill, Jim Sibthorp

Research in Outdoor Education

This study, with the help of the Utah Association of Diabetes Educators, attempted to determine the effectiveness of using self-determination theory (SDT) at an adolescent diabetes camp to increase internalization of diabetes management. Existing evidence indicates that adolescents manage less than 20% of their diabetes, far less than needed (Anderson, 1985). Failure to manage and internalize behavior for regulating glycemic levels can lead to a number of very significant problems (Bryden, Peveler, Stein, Neil, Mayou & Dunger, 2001). This limited internalized behavior·may be explained by SDT. Self determination theory provides a basis for creating an autonomy supportive. environment while fostering …

Pyridoxamine Traps Intermediates In Lipid Peroxidation Reactions In Vivo: Evidence On The Role Of Lipids In Chemical Modification Of Protein And Development Of Diabetic Complications, Thomas O. Metz, Nathan L. Alderson, Mark E. Chachich, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

Maillard or browning reactions between reducing sugars and protein lead to formation of advanced glycation end products (AGEs) and are thought to contribute to the pathogenesis of diabetic complications. AGE inhibitors such as aminoguanidine and pyridoxamine (PM) inhibit both the formation of AGEs and development of complications in animal models of diabetes. PM also inhibits the chemical modification of protein by advanced lipoxidation end products (ALEs) during lipid peroxidation reactions in vitro. We show here that several PM adducts, formed in incubations of PM with linoleate and arachidonate in vitro, are also excreted in the urine of PM-treated animals. The …

Age-Dependent Increase In Ortho-Tyrosine And Methionine Sulfoxide In Human Skin Collagen Is Not Accelerated In Diabetes: Evidence Against A Generalized Increase In Oxidative Stress In Diabetes, Mary C. Wells-Knecht, Timothy J. Lyons, David R. Mccance, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

The glycoxidation products Nepsilon-(carboxymethyl)lysine and pentosidine increase in skin collagen with age and at an accelerated rate in diabetes. Their age-adjusted concentrations in skin collagen are correlated with the severity of diabetic complications. To determine the relative roles of increased glycation and/or oxidation in the accelerated formation of glycoxidation products in diabetes, we measured levels of amino acid oxidation products, distinct from glycoxidative modifications of amino acids, as independent indicators of oxidative stress and damage to collagen in aging and diabetes. We show that ortho-tyrosine and methionine sulfoxide are formed in concert with Nepsilon-(carboxymethyl)lysine and pentosidine during glycoxidation of collagen …

Enhanced Nonenzymatic Glucosylation Of Human Serum Albumin In Diabetes Mellitus, C. Earl Guthrow, Mary Ann Morris, James F. Day, Suzanne R. Thorpe, John W. Baynes

Faculty Publications

Use of an ion exchange chromatographic method and a colorimetric method with thiobarbituric acid showed that levels of nonenzymatically glucosylated serum albumin were increased in patients with poorly controlled diabetes mellitus compared to controls. The two methods correlated well (r = 0.99) and clearly discriminated between normal and poorly controlled diabetic populations. The levels of glycosylated hemoglobin were also measured in both populations. Several patients apparently in good control based on glycosylated hemoglobin measurements were found to have increased levels of glycosylated albumin. Because albumin has a shorter circulating half-life than does the human erythrocyte, the plasma concentration of glucosylated …