Digital Commons Network^™

Ambiguity In Statistical Analysis Methods And Nonconformity With Prespecified Commitment To Data Sharing In A Cluster Randomized Controlled Trial, Yasaman Jamshidi-Naeini, Lilian Golzarri-Arroyo, Deependra K Thapa, Andrew W Brown, Daniel E Kpormegbey, David B Allison

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Fruit And Vegetable Intake, Food Security, Barriers To Healthy Eating, And Empowerment Among Dietetic Interns And Physician Assistant Interns: A Cross-Sectional Pilot Study, Alison Campbell, Maren Stirling, Natalie Keele, Alisse Larkin, Rachel Crandall, Aubree N. Wilcox, Meghan Adair, Cloie Malan, Jaidyn Thomson, Alexis Bennett, Heather Jensen, Hadyn Flygare, Lacie Peterson, Holly Hopkins, Nikki Kendrick, Maria Givler, Rebecca Charlton, Katie N. Kraus

Nutrition, Dietetics, and Food Sciences Faculty Publications

Students are required to complete supervised practice hours prior to becoming Registered Dietitians and Physician Assistants. Research suggests that environmental and social factors affect dietetic interns’ diets during their internship, although these factors have not been studied among physician assistant interns. This cross-sectional study utilized an online survey to compare dietetic interns’ (n = 81) and physician assistant interns’ (n = 79) fruit and vegetable intake, food security, barriers to healthy eating, and empowerment for making healthy dietary choices during an internship. Differences were assessed via independent t-tests and chi-square distributions. The significance was set at p …

Nhm 728: Advanced Survey Data Analysis, Yunhee Chang

GMAS Course Syllabi

No abstract provided.

Nourishtank: An Innovative Program On Solving Hunger, Palak Gupta, Ashley Sullivan, Kristin Hoch, Lea Palmer, Jocelin Gibson, Mateja Savoie-Roskos, Heidi Leblanc

Outcomes and Impact Quarterly

NourishTank, a Shark Tank-style event, saw students propose food and nutrition insecurity solutions. Twenty-eight students formed 11 teams, promoting interdisciplinary collaboration. The winning idea, 'Farm2Fork Gone Mobile,' earned $1,000 toward their proposed project. NourishTank, through partnerships and media, highlights student-driven efforts combating food insecurity and raising awareness.

A Mindful And Sustainable Eating Intervention To Improve Attitudes Related To Mindfulness, Sustainability, And Fruit And Vegetable Consumption In Elementary Schools, Amy Schachtner-Appel, Heejung Song

Journal of Human Sciences and Extension

Excessive plate waste is generated in elementary school cafeterias. Adapting nutrition promotion strategies to target waste reduction and encouraging mindful and sustainable eating (MSE) are possible strategies to encourage students to reduce waste during school lunch. Farm to Tray, Tray to Farm is a 16-week intervention that aims to encourage elementary school students to develop mindful and sustainable eating behaviors. An educational curriculum was delivered to eight 5th-grade students, who served as peer leaders and disseminated messages throughout the school. Additional strategies included cafeteria reinforcements and opportunities for food recovery. A pilot test of the program was conducted in two …

Total Syntheses Of Hosieines A-C, Jiayang Zhang, Xu Yan, Qing-Bao Zhang, Fang Wang, Bin Yang, Yang Yang

Children’s Nutrition Research Center Staff Publications

The collective total syntheses of (±)-hosieines A-C with a cage-like tetracyclic framework have been realized, which includes the first syntheses of hosieines B-C. The key strategy of the synthesis employs a one-pot domino reaction that involves Cu-catalyzed [3+2] cycloaddition, 1,6-enone formation, and 1,6-aza-Michael addition forming the 5/6/6-aza-tricyclic skeleton. Other salient synthetic tactics comprise a challenging double bond migration and a 1,4-aza-Michael addition reaction to afford the tetracyclic framework.

Locally Tailored Knowledge, Attitude And Practice Towards Diet Sustainability Questionnaire: Validation Among Uaeu Adults, Asma Bahaaldeen Numan

Theses

Food production is a major contributor to global environmental change and sustainability. Sustainable diets can be defined as “diets with low environmental impacts which contribute to food and nutrition security and healthy life for present and future generations”. The United Arab Emirates (UAE) aims to enhance its food security to meet the current local demand and future needs of an increasing population. Before determining a sustainable diet pattern for the UAE population, it is foremost more important to understand the perception of sustainable diet production and consumption. A thorough comprehension of the knowledge, attitudes, and practices of the United Arab …

Una Exploración Sobre La Alimentación Saludable. Percepciones De Mujeres Adultas Entre 31 Y 45 Años Según Su Nivel De Ingresos En La Ciudad Autónoma De Buenos Aires En 2024 / An Exploration Of Healthy Eating. Perceptions Of Adult Women Between 31 And 45 Years Old According To Their Income Level In The Autonomous City Of Buenos Aires In 2024, Anabelle Flaharty

Independent Study Project (ISP) Collection

En los últimos años, Argentina ha identificado los hábitos alimentarios como un área crítica que necesita análisis e intervención. Dado que la desnutrición es una de las principales causas de enfermedades no transmisibles (ENTs), organizaciones mundiales de salud han afirmado que practicar una “alimentación saludable” es una solución práctica a este problema. Entre la población argentina, la alimentación poco saludable ha provocado una epidemia de obesidad, sobrepeso y ENTs. Se sabe que hoy el 31,7 % de las mujeres argentinas adultas y el 30,2 % de los hombres argentinos adultos tienen sobrepeso. Además, la diabetes tiene una prevalencia del 10,5% …

Long-Term Health Related Quality Of Life Following Uterine Fibroid Embolization In A Predominantly Black African Population: A Retrospective Cohort Study, Michael Kioko, Marleen Temmerman, Timona Obura, Adelaide Lusambili

Obstetrics and Gynaecology, East Africa

Uterine Fibroid Embolization (UFE) is one of the effective options available for treatment of symptomatic uterine fibroids with documented improvement in the quality of life and reduction in symptoms. The study assessed long-term quality of life post-uterine fibroid embolization in a mostly black population, noting scanty local or African research on this topic despite evidence suggesting a higher fibroid burden among black women. This retrospective study examined patients who underwent UFE from 2009 to 2014. Participants completed online demographic and UFS-QOL surveys. Statistical tests included Wilcoxon signed rank tests for HRQOL score differences and Pearson correlation for associations between independent …

Design, Protocol And Baseline Data Of Nurturing Healthy Teachers, A Cluster Non-Randomized Controlled Trial To Improve The Health, Well-Being, And Food Security Of Preschool And Elementary School Teachers In Houston, Texas, Shreela V Sharma, Mackenzie Senn, Angela Zieba, Miao Tang, Ru-Jye Chuang, Courtney Byrd-Williams, Mike Pomeroy, Azar Gaminian, Jill Cox, Katherine French, Nalini Ranjit

Faculty, Staff and Student Publications

BACKGROUND: We present the conceptual framework, design, and study measures of Nurturing Healthy Teachers, a quasi-experimental study to examine the short- and long-term effectiveness of the Nurturing Healthy Teachers (NHT) nutrition intervention on food insecurity, dietary behaviors, mental health and cardiometabolic health among preschool and elementary school teachers.

METHODS: A convenience sample of 28 elementary schools with pre-kindergarten and elementary classrooms were recruited in Houston, Texas. Nurturing Healthy Teacher intervention includes Brighter Bites, an evidence-based coordinated school health program that combines access to fresh produce and nutrition education, and Create Healthy Futures, a web-based nutrition education program that targets nutrition …

Serum Metabolomic Markers Of Protein-Rich Foods And Incident Ckd: Results From The Atherosclerosis Risk In Communities Study, Lauren Bernard, Jingsha Chen, Hyunju Kim, Kari E Wong, Lyn M Steffen, Bing Yu, Eric Boerwinkle, Andrew S Levey, Morgan E Grams, Eugene P Rhee, Casey M Rebholz

Faculty, Staff and Student Publications

RATIONALE & OBJECTIVE: While urine excretion of nitrogen estimates the total protein intake, biomarkers of specific dietary protein sources have been sparsely studied. Using untargeted metabolomics, this study aimed to identify serum metabolomic markers of 6 protein-rich foods and to examine whether dietary protein-related metabolites are associated with incident chronic kidney disease (CKD).

STUDY DESIGN: Prospective cohort study.

SETTING & PARTICIPANTS: A total of 3,726 participants from the Atherosclerosis Risk in Communities study without CKD at baseline.

EXPOSURES: Dietary intake of 6 protein-rich foods (fish, nuts, legumes, red and processed meat, eggs, and poultry), serum metabolites.

OUTCOMES: Incident CKD (estimated …

Nutri-Score, Warning Signs, And Facts Up Front – In Theory And In Practice A Comparative Analysis Of The Effectiveness Of Front-Of-Package Labeling Systems, Muskaan Makkar

Independent Study Project (ISP) Collection

Suboptimal diets contribute significantly to poor health and chronic diseases, with excessive sodium intake and low consumption of whole grains, fruits, and vegetables being major risk factors. To address this global nutrition burden, various countries have implemented front-of-package labeling (FOPL) systems such as the Nutri-Score, Warning Signs, and Facts Up Front.

This paper aims to analyze the effectiveness of these systems – which vary in development, design, content, and implementation – in both theory and practice. The methodology involves a comparative analysis of three FOPL systems, assessing their theoretical effectiveness with international guidelines and practical effectiveness through expert interviews and …

Lac-Phe Mediates The Effects Of Metformin On Food Intake And Body Weight, Shuke Xiao, Veronica L Li, Xuchao Lyu, Xudong Chen, Wei Wei, Fahim Abbasi, Joshua W Knowles, Alan Sheng-Hwa Tung, Shuliang Deng, Gaurav Tiwari, Xu Shi, Shuning Zheng, Laurie Farrell, Zsu-Zsu Chen, Kent D Taylor, Xiuqing Guo, Mark O Goodarzi, Alexis C Wood, Yii-Der Ida Chen, Leslie A Lange, Stephen S Rich, Jerome I Rotter, Clary B Clish, Usman A Tahir, Robert E Gerszten, Mark D Benson, Jonathan Z Long

Children’s Nutrition Research Center Staff Publications

Metformin is a widely prescribed anti-diabetic medicine that also reduces body weight. There is ongoing debate about the mechanisms that mediate metformin’s effects on energy balance. Here, we show that metformin is a powerful pharmacological inducer of the anorexigenic metabolite N-lactoyl-phenylalanine (Lac-Phe) in cells, in mice and two independent human cohorts. Metformin drives Lac-Phe biosynthesis through the inhibition of complex I, increased glycolytic flux and intracellular lactate mass action. Intestinal epithelial CNDP2+ cells, not macrophages, are the principal in vivo source of basal and metformin-inducible Lac-Phe. Genetic ablation of Lac-Phe biosynthesis in male mice renders animals resistant to the …

The Impact Of The Gut-Brain Axis On Alzheimer’S Disease, Elissa Wakim

Best Integrated Writing

Elissa’s review for the Graduate Biomedical Review focuses on the links between the gastrointestinal tract and the brain; the gut-brain axis and the development of Alzheimer’s disease. As a student in the Microbiology and Immunology Masters Program Elissa was particularly interested in the gut microbiota and their connection to neurodegenerative disease. She tidily reviewed the literature and wrote a fascinating and compelling piece of work.

Best Integrated Writing Spring 2024 - Complete Edition, Wright State University School Of Humanities And Cultural Studies, Kristie Mckiernan, Tracy Smith

Best Integrated Writing

Best Integrated Writing includes excellent student writing from Integrated Writing courses taught at Wright State University. This is the first issue after a 5 year hiatus.

Dissection, Histological Processing, And Gene Expression Analysis Of Murine Supraclavicular Brown Adipose Tissue, Mark G Waterstraat, Ziyi Wang, Mari Kogiso, Rommel Caballero-Juarez, Miao-Hsueh Chen

Children’s Nutrition Research Center Staff Publications

Brown adipose tissue (BAT)-mediated thermogenesis plays an important role in the regulation of metabolism, and its morphology and function can be greatly impacted by environmental stimuli in mice and humans. Currently, murine interscapular BAT (iBAT), which is located between two scapulae in the upper dorsal flank of mice, is the main BAT depot used by research laboratories to study BAT function. Recently, a few previously unknown BAT depots were identified in mice, including one analogous to human supraclavicular brown adipose tissue. Unlike iBAT, murine supraclavicular brown adipose tissue (scBAT) is situated in the intermediate layer of the neck and thus …

Dissection, Histological Processing, And Gene Expression Analysis Of Murine Supraclavicular Brown Adipose Tissuejove: Peer Reviewed Scientific Video Journal Article Protocols, Mark G Waterstraat, Ziyi Wang, Mari Kogiso, Rommel Caballero-Juarez, Miao-Hsueh Chen

Faculty, Staff and Students Publications

Brown adipose tissue (BAT)-mediated thermogenesis plays an important role in the regulation of metabolism, and its morphology and function can be greatly impacted by environmental stimuli in mice and humans. Currently, murine interscapular BAT (iBAT), which is located between two scapulae in the upper dorsal flank of mice, is the main BAT depot used by research laboratories to study BAT function. Recently, a few previously unknown BAT depots were identified in mice, including one analogous to human supraclavicular brown adipose tissue. Unlike iBAT, murine supraclavicular brown adipose tissue (scBAT) is situated in the intermediate layer of the neck and thus …

Nkx6.1 And Stx1a As Binding Partners In Pancreatic Beta Cells, Jakob T. Lenker, Jeff S. Tessem, Connor C. Littlefield

Library/Life Sciences Undergraduate Poster Competition 2024

Diabetes is characterized by a loss in beta cell function within the pancreas and the subsequent inability to produce sufficient insulin to regulate blood glucose. While current diabetes treatments focus on delivering pharmaceutical insulin to diabetic individuals, such treatments are temporary solutions and do not address the root of the issue. Instead, our research focuses on potential mechanisms for inducing greater insulin secretion within the pancreas of the individual. NK6 Homeobox 1 (Nkx6.1) is a major transcription factor in beta cells and its overexpression in beta cells is associated with higher insulin secretion. Previous research indicates that Syntaxin 1A (Stx1A) …

Pilot Study To Assess The Effectiveness Of The Sustainable Culturally Adaptive Nutrition Program (Scan) To Improve Adherence To The National Diabetes Prevention Program, William B Perkison, Pierre Fwelo, Fernanda Velasco-Huerta, Natalia I Heredia, James J Yang, Sidra S Beg, Belinda M Reininger, Serena A Rodriguez, Maha Almohamad, Catherine Pulicken, Ella Garza, Grace E White, Maria E Fernandez

Faculty, Staff and Student Publications

PURPOSE: The Sustainable Culturally Adapted Nutrition Program (SCAN) is a novel adaptation to the National Diabetes Prevention Program (NDPP) that aims to improve attendance and effectiveness. This paper presents its feasibility and impact through the initial 6-month outcomes.

DESIGN: A pragmatic quasi-experimental pilot study with intervention (DPP plus SCAN) and control (DPP only) groups.

SAMPLES AND INCLUSION CRITERIA: Sustainable Culturally Adapted Nutrition Program participants were recruited from federally qualified health center (FQHC) clinic patients enrolled in a NDPP in Houston, Texas. Participants needed to be (1) ≥18 years old, (2) body mass index >25, (3) no prior diagnosis of diabetes, …

Beyond Translation: Increasing Accessibility To Nutrition Education For Foreign-Born Latine Adults, Karen L. Franck, Jennifer Ward, Lisa Fuller, Egla Delvo-Lopez, Chanta'l Rose

Journal of Extension

About 20% of the U.S. population identifies as Latine. To engage these audiences, many Extension nutrition education programs have translated curricula and other resources into Spanish. Culturally responsive programs need to move beyond translation and address critical issues and needs specific to Latine audiences. We conducted focus groups with 118 foreign-born Latine adults across one state. Participants were interested in nutrition education programs involving the entire family including children offered by bilingual educators. They also wanted programs that focused on healthy meals that included foods from their culture of origin with common US foods.

A Nutritional Intervention To Improve Body Composition In Adolescent Athletes, Michael Ryan, Rosemary E. Borgerding, Kimberly L. Oliver

Journal of Athlete Development and Experience

This study assessed the effectiveness of a sports nutrition intervention designed to improve body composition in 25 high-school male athletes over a 12-week period. Employing a mixed-methods approach within a quasi-experimental one-group pretest-posttest design, the intervention integrated nutrition education with the provision of food. Quantitative results revealed significant reductions in body fat percentage and fat mass, and an increase in fat-free mass. Qualitative insights highlighted the untapped nutritional potential of adolescents and emphasized the importance of accessibility and simplicity in driving compliance. The findings suggest that targeted nutritional interventions can positively impact adolescent athletes' body composition. The study underscores the …

Trends In Us Public Confidence In Science And Opportunities For Progress, Arthur Lupia, David B Allison, Kathleen Hall Jamieson, Jennifer Heimberg, Magdalena Skipper, Susan M Wolf

Children’s Nutrition Research Center Staff Publications

In recent years, many questions have been raised about whether public confidence in science is changing. To clarify recent trends in the public's confidence and factors that are associated with these feelings, an effort initiated by the National Academies' Strategic Council for Research Excellence, Integrity, and Trust (the Strategic Council) analyzed findings from multiple survey research organizations. The Strategic Council's effort, which began in 2022, found that U.S. public confidence in science, the scientific community, and leaders of scientific communities is high relative to other civic, cultural, and governmental institutions for which researchers regularly collect such data. However, confidence in …

Association Of Habitual Intake Of Probiotic Supplements And Yogurt With Characteristics Of The Gut Microbiome In The Multiethnic Cohort Adiposity Phenotype Study, Weiwen Chai, Gertraud Maskarinec, Unhee Lim, Carol J. Boushey, Lynne R. Wilkens, V. Wendy Setiawan, Loïc Le Marchand, Timothy W. Randolph, Isaac C. Jenkins, Johanna W. Lampe, Meredith A.J. Hullar

Department of Nutrition and Health Sciences: Faculty Publications

Consumption of probiotics and/or yogurt could be a solution for restoring the balance of the gut microbiota. This study examined associations of regular intake of probiotic supplements or yogurt with the gut microbiota among a diverse population of older adults (N=1,861; 60–72 years). Fecal microbial composition was obtained from 16S rRNA gene sequencing (V1-V3 region). General Linear Models were used to estimate the associations of probiotic supplement or yogurt intake with microbiome measures adjusting for covariates. Compared to non-yogurt consumers (N=1,023), regular yogurt consumers (≥once/week, N=818) had greater Streptococcus (β=0.29, P=0.0003) and lower Odoribacter (β=−0.33, P<0.0001) abundance. The directions of the above associations were consistent across the five ethnic groups but stronger among Japanese Americans (Streptococcus: β=0.56, P=0.0009; Odoribacter: …

Urban-Rural Differences In School Districts' Local Wellness Policies And Policy Implementation Environments, Swati Iyer, Timothy J Walker, Alexandra L Macmillan Uribe, Chad D Rethorst, Rebecca A Seguin-Fowler, Jacob Szeszulski

Faculty, Staff and Student Publications

Higher rates of obesity in rural compared to urban districts suggest environmental differences that affect student health. This study examined urban-rural differences in districts' local wellness policies (LWPs) and LWP implementation environments. Cross-sectional data from two assessments in Texas were analyzed. In assessment one, each district's LWP was reviewed to see if 16 goals were included. In assessment two, an audit was conducted to identify the presence of a wellness plan (a document with recommendations for implementing LWPs), triennial LWP assessment, and school health advisory councils (SHACs) on the district website. Rural districts' LWPs had a smaller number of total …

Assessing The Reliability, Internal Consistency, And Sensitivity Of A Nutrition Knowledge Questionnaire For Four-Year-Old Pre-K Children, Lin Wang, Karla Cortez, Brenda Rodriguez, Joseph Reyes, Moises Cisneros, Elizabeth Alanis, Zasha Romero, Lisa Michelle Belzer Salinas, Juan López Alvarenga, Roberto Treviño-Peña

Research Symposium

Introduction: Assessing nutrition knowledge in four-year-olds, a developmental stage marked by limited attention spans and varying comprehension abilities, is challenging with traditional methods. A reliable test is pivotal for establishing a foundation for future health interventions. We evaluated a 13-question nutrition and healthy habits test in Pre-K4 children to assess reliability and sensitivity to detect differences.

Methods: Calculations included Cronbach's alpha, kappa coefficient, McNemar analysis by item, and Bland-Altman plots for test-retest differences. Mixed model regression assessed the questionnaire's sensitivity by sex and association with age. Item response theory (IRT) models were employed, generating latent abilities for students and individual …

Optimal Growth And Development: Are Teenagers Getting Enough Micronutrients From Their Diet?, Niamh M. Walsh, Albert Flynn, Janette Walton, Laura Kehoe

Department of Biological Sciences Publications

The teenage years represent a crucial period of physical and cognitive growth and development with sufficient micronutrient intakes necessary to meet high nutritional requirements. This review examines current micronutrient intakes in teenagers in the Western world in the context of public health implications including the prevalence of inadequate intakes and risk of excessive intakes. Intakes of vitamins A, D, E and C, folate, calcium, iron, magnesium, zinc and potassium in teenagers are low when compared to generally accepted recommendations, while there is little risk of excessive micronutrient intakes based on current dietary patterns. Therefore, strategies should focus on increasing micronutrient …

A Dietary Variety: Analyzing The Impacts Of Diverse Vs Worm-Exclusive Diets On The Blanding’S Turtle (Emydoidea Blandingii), Caitlin F. Copenhaver

Honors Program: Senior Projects (Public)

The endangered Blanding’s turtle (Emydoidea blandingii) is a species with a generally diverse diet in their freshwater wetland habitats; they consume prey such as crayfish and snails. In captivity, these options often become more limited due to logistical reasons. As ex situ conservation efforts such as captive rearing and head-starting are becoming more popular for this species, it will be important to gain more insight on how these more limited diets might affect these turtles. This study describes a comparison between diets in six captive Blanding’s turtles from September to November of 2023, with three turtles receiving only …

Effects Of Black Raspberry, Blueberry, And Blackberry Volatile Extracts On Intestinal Barrier Dysfunction And Inflammation In A Caco-2/Raw264.7 Cells Co-Culture Model, Inah Gu, Cindi Brownmiller, Luke Howard, Sun-Ok Lee

Food Science Faculty Publications and Presentations

Berry volatiles are responsible for the aroma of berries. However, their impacts on intestinal inflammation are unclear. Thus, this study investigated the effects of black raspberry, blueberry, and blackberry volatile extracts (BVEs) on intestinal barrier dysfunction and inflammation using a Caco-2/RAW264.7 cells co-culture model. BVE treatments significantly enhanced integrity and decreased permeability in LPS-damaged epithelial monolayers with increased tight junctions. Blueberry and blackberry volatile extract treatments significantly inhibited the LPS-induced overproduction of NO, IL-6, and TNF-α (p < 0.05). BVEs also suppressed the LPS-induced phosphorylation of p65, IKKα, and IκBα. These findings revealed that volatile extracts from black raspberries, blueberries, and blackberries improve intestinal barrier dysfunction by enhancing intestinal epithelial monolayer integrity and exhibit intestinal anti-inflammatory effects by suppressing pro-inflammatory mediator and cytokines through modulating NF-κB signaling pathway. It suggests that the three berry volatile extracts have the potential as functional food components with gastrointestinal protective effects with anti-inflammatory properties.

Advances And Challenges In Conventional And Modern Techniques For Halal Food Authentication: A Review, Ifrah Usman, Saima Sana, Muhammad Afzaal, Ali Imran, Farhan Saeed, Aftab Ahmed, Yasir Abbas Shah, Muniba Munir, Huda Ateeq, Atka Afzal, Iqra Azam, Afaf Ejaz, Gulzar Ahmad Nayik, Mahbubar Rahman Khan

Faculty, Staff and Student Publications

Food is one of the most necessary needs since human civilization. For Muslims, it is mandatory to consume halal food. From a halal authentication perspective, adulteration of food products is an emerging challenge worldwide. The demand for halal food consumption has resulted in an ever-increasing need for halal product validity. In the market, there are several food products in which actual ingredients and their source are not mentioned on the label and cannot be observed by the naked eye. Commonly nonhalal items include pig derivatives like lard, pork, and gelatin derivatives, dead meats, alcohol, blood, and prohibited animals. Purposely, various …

Targeting Glutamine Dependence With Drp-104 Inhibits Proliferation And Tumor Growth Of Castration-Resistant Prostate Cancer, David Moon, J Spencer Hauck, Xue Jiang, Holly Quang, Lingfan Xu, Fan Zhang, Xia Gao, Robert Wild, Jeffrey I Everitt, Everardo Macias, Yiping He, Jiaoti Huang

Faculty, Staff and Students Publications

BACKGROUND: Prostate cancer (PCa) continues to be one of the leading causes of cancer deaths in men. While androgen deprivation therapy is initially effective, castration-resistant PCa (CRPC) often recurs and has limited treatment options. Our previous study identified glutamine metabolism to be critical for CRPC growth. The glutamine antagonist 6-diazo-5-oxo-l-norleucine (DON) blocks both carbon and nitrogen pathways but has dose-limiting toxicity. The prodrug DRP-104 is expected to be preferentially converted to DON in tumor cells to inhibit glutamine utilization with minimal toxicity. However, CRPC cells' susceptibility to DRP-104 remains unclear.

METHODS: Human PCa cell lines (LNCaP, LAPC4, C4-2/MDVR, PC-3, 22RV1, …