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Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

Some Characteristics Of A Purified Heat-Stable Aldolase, P.J. Thompson, T.L. Thompson

Papers in Microbiology

THOMPSON, P. J. (University of Nebraska, Lincoln) AND T. L. THOMPSON. Some characteristics of a purified heat-stable aldolase. J. Bacteriol. 84:694-700. 1962-Aldolase from a thermophilic strain of bacteria was obtained in a state of high purity. Heat studies of purified aldolases from cells cultivated at 45 and 65 C showed them equally stable at 70 C for 1 hr. Metal-ion and chelate studies indicated that thermal aldolase is metal ion-independent. Carboxypeptidase did not alter activity or specificity. The enzyme was specific for fructose- 1, 6-diphosphate. Hydrazine was found inhibitory in the assay procedure. The inhibition was independent of pH over …

Pullorum Tested Poultry Flocks : 1962, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

THIS year's pullorum tests of poultry flocks have revealed the lowest incidence of reactors since the tests began.

Results are recorded below.

Natural History Of Thelazia Californiensis Price, 1930 (Nematoda: Thelaziidae), A Mammalian Eye Worm, Warren E. Parmelee

Loma Linda University Electronic Theses, Dissertations & Projects

Various factors—embryology, host associations, and seasonal changes In eye worn populations—tending to promote the propagation of T. califomlensls were studied.

The early embryology closely parallels that of other nematode species.

The mature embryos remain alive in saline at 35°C as long as 5 days. Dead embryos can be distinguished from living ones by virtue of the rapid staining by methylene blue by the former. Parturient female thelazias release embryos into saline, and embryos are found regularly in tear secretions of infected laboratory rabbits.

Ovic embryos possess an enveloping egg membrane containing chitin. On this account, this eye worm species is …

Natural And Experimental Infections Of Thelazia Californiensis Price (1930, Nematoda: Thelaziidae) With Descriptions Of The Adult And Larval Parasites, Howard S. Burnett

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this work was to determine the extent and effects of infestation of the eye worm of mammals, Thelazia californiensis, and to elucidate through experimentation or observation the mode of transmission.

A survey of literature revealed that thelaziasie (infestation by Thelazia species) may cause serious pathological changes in the eye and its adnexa, sometimes resulting in blindness. It was found that reports of thelaziasis in the United States were (with two exceptions) of cases in California. It was further found that T. californiensis has been reported in a wider variety of host than has any of other …

Pustule Formation By Lactobacilli On Fermented Vegetables, T.L. Thompson, W.E. Engelhard, Hilliard Pivnik

Papers in Microbiology

Genuine dill pickles are the product resulting from the fermentation, predominantly lactic, of cucumbers immersed in a brine containing 3 to 4 per cent salt. The brine also contains dill weed and may contain spices and garlic or extracts of their essential oils. Genuine dill tomatoes are manufactured from green tomatoes by a fermentation similar to that used for pickles (Fabian and Wadsworth, 1944).
Lactobacillus plantarum is commonly associated with the fermentation of pickles (Pederson, 1936), although other lactobacilli and some yeasts undoubtedly play a role. A survery of the literature failed to uncover any reports concerning the microbial flora …

Studies On The Validity Of Swine Erysipelas Culture-Vaccines, L. Van Es, J. F. Olney, I. C. Blore

Nebraska Agricultural Experiment Station: Historical Research Bulletins

In countries where vaccination against swine erysipelas has been practiced during the last half century, there is a consensus among veterinarians that the best results are obtained when the live culture-vaccine is not only fresh and highly virulent but is endowed also with a good capacity for growth on artificial culture media. Apparently avirulent culture-vaccines are apt to engender only a transitory immunity or fail altogether. The problem presented by undesirable culture-vaccines was deemed to be of such importance to the swine industry that this Station undertook a series of experiments, to determine what influences might be accountable for the …

Two Lincoln (Nebraska) Typhoid Fever Epidemics Of 1911 And 1912., Herbert H. Waite

School of Biological Sciences: Faculty Publications

On August 29, 1912, I was requested by the Board of Health of the city of Lincoln to make an investigation to determine if possible the origin and cause of an outbreak of a disease reported as typhoid fever which was then prevailing. This outbreak was said to be most prevalent in one particular portion of the city and consequently attention was turned to that portion. Of the cases reported an investigation was made of over 60 individuals and after considering all the probable causes for the majority of those affected the conclusion was reached that all could be logically …

Replicating Single-Cycle Adenovirus Vectors Generate Amplified Influenza Vaccine Responses, Catherine M. Crosby, William E. Matchett, Stephanie S. Anguiano-Zarate, Christopher A. Parks, Eric A. Weaver, Larry R. Pease, Richard J. Webby, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Head-to-head comparisons of conventional influenza vaccines with adenovirus (Ad) gene-based vaccines demonstrated that these viral vectors can mediate more potent protection against influenza virus infection in animal models. In most cases, Ad vaccines are engineered to be replication-defective (RD-Ad) vectors. In contrast, replication-competent Ad (RC-Ad) vaccines are markedly more potent but risk causing adenovirus diseases in vaccine recipients and health care workers. To harness antigen gene replication but avoid production of infectious virions, we developed “single-cycle” adenovirus (SC-Ad) vectors. Previous work demonstrated that SC-Ads amplify transgene expression 100-fold and produce markedly stronger and more persistent immune responses than RD-Ad vectors …