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Purification Of Maturation-Promoting Factor, An Intracellular Regulator Of Early Mitotic Events, Manfred J. Lohka, Marianne K. Hayes, James L. Maller

School of Veterinary and Biomedical Sciences: Faculty Publications

Maturation-promoting factor causes germinal vesicle breakdown when injected into Xenopus oocytes and can induce metaphase in a cell-free system. The cell-free assay was used to monitor maturation-promoting factor during its purification from unfertilized Xenopus eggs. Ammonium sulfate precipitation and six chromatographic procedures resulted in a preparation purified >3000-fold that could induce germinal vesicle breakdown within 2 hr when injected into cycloheximide- treated oocytes. Proteins of 45 kDa and 32 kDa were correlated with fractions of highest activity in both assays. These fractions contained a protein kinase activity able to phosphorylate the endogenous 45-kDa protein, as well as histone Hi, phosphatase …

Sensitivity To Ingested Sulfites, Steve L. Taylor, Julie A. Nordlee

Department of Food Science and Technology: Faculty Publications

Sulfiting agents, including sodium and potassium bisulfite, sodium and potassium metabisulfite, sodium sulfite, and sulfur dioxide, have enjoyed widespread use as food and drug ingredients. The oral ingestion of these sulfiting agents is now known to trigger asthma in a small subset of the asthmatic population. The best evidence suggests that perhaps 150,000 to 200,000 individuals in the United States may be sulfite sensitive. Although the mechanism of sulfite-induced asthma remains unknown, several possibilities have been considered, including inhalation of sulfur dioxide (SO₂) while swallowing, an IgE-mediated reaction, and a deficiency of sulfite oxidase leading to impaired sulfite …

Visually Displayed-Emg Biofeedback : Training Muscle Relaxation In Hearing Impaired Children :A Thesis ..., Cheryl Renee Bene

University of the Pacific Theses and Dissertations

The purpose of the present study was to test the use of visually displayed EMG biofeedback as a means for training hearing impaired adolescents to reduce anterior temporalis or frontalis muscle tension . Five male and four female hearing impaired students between the ages of 13 and 15 were chosen from the California School for the Deaf, Fremont, CA to serve as participants. Each participant was randomly assigned to either an experimental or control condition. Participants in the experimental groups were given five 15 minute EMG biofeedback training sessions. An additional group of 4 adolescents with normal hearing from Marshall …

Chromatographic And Mass Spectrometric Analysis Of Bradykinin Related Arginyl Peptides And Other Studies, Kevin Dale Ballard

MUSC Theses and Dissertations

Dimethylpyrimidylornithyl-N-trifluoroethyl-O-TBDMS (DMPO-N-TFE-O-TBDMS) polyaminoalcohol derivatives of arginyl peptides were evaluated for their utility in peptide analysis by capillary gas chromatography-mass spectrometry (GC-MS). These derivatives were hydrolytically stable and sufficiently volatile for capillary GC-MS analysis of low nanomole amounts of peptides as large as RPPGF. Complete amino acid sequence information was generated under electron impact ionization conditions, including several ions diagnostic for arginine residues, with molecular weight information imparted by the [M-15]+ and [M-57]+ pair of ions characteristic of TBDMS derivatives. Analysis of the sequential intermediate derivatives by FAB-MS facilitated optimization of problematic derivatization steps. As little as 20 picomoles of peptide …

Use Of Circadian Rhythms To Screen For Fever: A Replication, Michele S. Heckman

Masters Theses

There are inconsistent guidelines for, and consequently, inconsistent practice in the screening of hospitalized adults for fever. Using the predictive knowledge of circadian thermal rhythms, the purposes of this study were to identify (1) the hour of the day in which most fevers occurred and (2) the necessary frequency of measurement to detect fever. The sample consisted of 35 subjects, ages 22-62. Temperatures were measured at 6 p.m., 10 p.m., 6 a.m., 10 a.m., 2 p.m., and 6 p.m. for 24 hours. Eighteen (51.42%) of the 35 subjects had fevers during the 24-hour measurement period. Fourteen (77.78%) initial fevers were …

Promoting Adaptation Of The Young Child During An Outpatient Surgical Admission Assessment, Mary C. Mitus

Masters Theses

This study used an experimental design to investigate whether the timing of a child's admission physical assessment, either before or after an interview with the child's parent(s), had an effect on the upset and cooperation behaviors exhibited by the child during the physical exam.; Thirty-two children, age 12 to 36 months, who were scheduled for elective outpatient surgery were randomly assigned to control and experimental groups.; The hypothesis of the study was that children who were examined after the interview would exhibit less upset behaviors and be more cooperative with the examining nurse. It was hypothesized that these children would …

Evaluating Cholesterol Screening In A Community Pharmacy, Osama Mohamed Ibrahim

University of the Pacific Theses and Dissertations

The purpose of this research project was to evaluate the role of the community pharmacist in screening, identifying, and referring ambulatory patients with high total blood cholesterol (TBC) in a community pharmacy. Fifty seven patients, out of 241 initially screened individuals, met the study inclusion criteria and were accepted into this study. Of these 57 patients, 51 patients completed the six month study period. The normal population group consisted of 164 participants with TBC < 200 mgjdL at the initial cholesterol testing (visit 1). The drop out group represented six patients who failed to continue attending the two follow up tests (visit 2 and 3). For screening purposes, a non-fasting whole blood sample was used to measure TBC using the Boehringer Mannheim Reflotron analyzer.

The project was evaluated based on mean TBC levels obtained during the initial screening and the two follow up tests, pre-test and post-test scores, behavior and …

Polyamine And Acetylpolyamine Levels During Phenylhydrazine-Induced Erythropoiesis In Mouse Spleen, Othman A. Alshabanah

University of the Pacific Theses and Dissertations

The phenylhydrazine-induced erythropoietic mouse spleen is used as a model system to demonstrate the relationship between tissue growth and polyamine metabolism. Phenylhydrazine produced significant changes in spleen weights, hematocrits and reticulocyte counts in Swiss-Webster mice. The average spleen weight went up from a control of 155 mg to 875 mg at 96 hours after phenylhydrazine administration, while a 49% reduction in the value of hematocrit was observed at 72 hours. Reticulocyte counts in peripheral blood went from 0.8 to 58% at 168 hours after treatment with phenylhydrazine. Phenylhydrazine at a dose of 40 mg/kg produced significant increases in the levels …

Differences In Coping Behaviors In Parents Of Mentally-Handicapped Children And Parents Of Nonhandicapped Children., Cynthia Anne Chatham

All ETDs from UAB

No abstract provided.

Parent Report Of Stressful Life Events, Social Support, And Children's Competence After Death Of A Significant Other., Sharon Naomi Leder

All ETDs from UAB

No abstract provided.

A Comparison Of Maternal Identity In Younger And Older Primiparae During The Third Trimester Of Pregnancy., Faye Hilley Shaffer

All ETDs from UAB

No abstract provided.

A Q Analysis Of The Stressors Of Fathers With An Infant In An Intensive Care Unit., Janice Lane Morris Vincent

All ETDs from UAB

No abstract provided.

Maternal Reactions To The Knowledge Of Fetal Sex Based On Sex Preference., Molly Kay Walker

All ETDs from UAB

No abstract provided.

Stressors, Levels Of Stress, And Self-Care Actions To Reduce Stress In Preadolescent Children., Linda Rollins Roberts

All ETDs from UAB

No abstract provided.

Autonomy, Coping, And Self-Care Agency In Healthy Adolescents And In Adolescents With Spina Bifida., Rita Black Monsen

All ETDs from UAB

No abstract provided.

1988 Winter No. 15, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 15 Winter 1988 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Clinical Use Of A Standard Kit-Preparation Of Radiolabeled Monoclonal Antibody 96.5 In The Diagnostic Imaging Of Metastatic Melanoma., Mansoor Saleh, D M. Miller, L Peterson, C D. Russell, M W. Unger

Haematology and Oncology, East Africa

We studied the efficiency of a standard-kit preparation using 1 mg 111In-labeled 96.5 monoclonal antibody in combination with 19 mg of unlabeled antibody in the diagnostic imaging of 27 patients with documented metastatic melanoma. Twenty-three of 26 patients (88%) demonstrated immunoscintigraphic localization of tumor. Of 104 metastatic sites previously documented by conventional studies, 62 (60%) were identified by immunoscintigraphy. A total of 77 sites demonstrated localization of radiolabeled antibody. Fifty-four (70%) corresponded to known sites of disease; eight sites (10%) were "discovered" by immunoscintigraphy and subsequently confirmed by conventional studies; 15 imaged sites (20%) could not be confirmed by conventional …

Role Strain For The Male Nursing Student In The Obstetrical Area., Roy Ann Sherrod

All ETDs from UAB

No abstract provided.

The Role Of Nurses In The United States Space Program., Pamela Gail Holder

All ETDs from UAB

No abstract provided.

The Business Of Caring: The Perspective Of Men In Nursing., Janice Ray Ingle

All ETDs from UAB

No abstract provided.

An Anatomical And Physiological Investigation Of Hippocampal Connections Involved In Cardiovascular Control, Kenneth Gary Ruit

Dissertations

No abstract provided.

Variations In Bracket Placement In The Preadjusted Orthodontic Appliance, Nasib Balut

Master's Theses

No abstract provided.

Pulpotomies Utilizing Povidone-Iodine In Primary Teeth, Ramon Marti

Master's Theses

No abstract provided.

Bond Strength Of The Ceramic Orthodontic Bracket-Adhesive Interface, Moon Woo Limb

Master's Theses

No abstract provided.

The Effects Of Relaxation Training On Anxiety Levels, Absenteeism, And Academic Performance In First, Second And Third Grade Children: Implications For Coping With Stress, So Hee Jon

Master's Theses

No abstract provided.

School Of Pharmacy, A History, Warren E. Weaver

VCU University History Books

Since the beginnings of the Medical College of Virginia (MCV), the School of Pharmacy at Virginia Commonwealth University has been a leader in pharmacy education. This book traces the history of the School of Pharmacy up to the time of its publication in 1988. Topics covered include the merging of MCV and University College of Medicine, the development of bachelors, masters and doctoral programs, changes in curriculum along with advances in the field, and the history of the school’s facilities.

Glucocorticoid-Responsive Hepatic Cytochromes P-450 In The Rat: Multiplicity And Differential Regulation, Keith Alan Hostetler

Theses and Dissertations

The multiplicity of glucocorticoid-responsive hepatic cytochromes P-450 in the rat was investigated by developing a series of monoclonal antibody probes directed against purified steroid-inducible isozymes from the rat (P-450p) and man (HLp). Two antibodies, termed 1G8 and 13-7-10, showed unique specificity for purified cytochromes P-450 upon immunoblot analyses. These antibodies were further characterized in terms of their reactivity toward proteins in liver microsomes from untreated and xenobiotic-treated rats. Three P-450p-related proteins were identified using 1G8 and 13-7-10 to monitor the expression of P-450p family subtypes. In untreated rats, members of this family exhibited sex-specific expression. Treatment with various chemicals resulted …

A Stereotaxic Atlas Of The Brain Of The Chick (Gallus Domesticus), Wayne J. Kuenzel, Manju Masson

Poultry Science Faculty Publications and Presentations

Nine brains taken from chicks two weeks of age were used for the development of this atlas. Each chick was first anesthetized with an intravenous (IV) injection of Chloropent2 (1.8 ml/kg). Chicks were then perfused via the heart with 90 ml physiological saline followed by 90 ml Heidenhain's3 solution. Each head was then positioned in a stereotaxic instrument as described in the previous section and three stainless steel (SS) insect pins (#2) were implanted in each brain at known coordinates. In the case of the brains used to construct the cross- sectional atlas plates, two pins were implanted horizontal to …

An Identified Histaminergic Neuron Can Modulate The Outputs Of Buccal-Cerebral Interneurons In Aplysia Via Presynaptic Inhibition, Hillel J. Chiel

Faculty Scholarship

We have identified 2 buccal-cerebral interneurons (BCIs), B17 and B18, that appear to be involved in the coordination of feeding behavior in Aplysia. The BCIs have their cell bodies in the buccal ganglion, but send axons to the cerebral ganglion via the cerebral-buccal connectives. The BCIs appear to make monosynaptic connections with neurons in the cerebral ganglion that modulate extrinsic muscles involved in feeding behavior. B17 and B18 are activated antiphasically during a motor program induced by stimulating the esophageal nerve and appear to 'read out' different phases of the buccal program to different cells in the cerebral ganglion. B17 …

Aspects Of Endocrinology, Michael Kleerekoper, Raymond C. Mellinger

Henry Ford Hospital Medical Journal

No abstract provided.