Digital Commons Network^™

T-Cell Responses To The M3 Immune Evasion Protein Of Murid Gammaherpesvirus 68 Are Partially Protective And Induced With Lytic Antigen Kinetics, Joshua J. Obar, Douglas C. Donovan, Sarah G. Crist, Ondine Silvia, James P. Stewart, Edward J. Usherwood

Dartmouth Scholarship

DNA vaccination with the M3 gene, encoding an immune evasion molecule expressed during both the acute lytic and persistent phases of murid gammaherpesvirus 68 infection, yielded a significantly lower titer of virus in the lung than controls. The protection seen was dependent on T cells, and we mapped an epitope recognized by CD8 T cells. The immune response to this epitope follows the same kinetics as lytic cycle antigens, despite the fact that this gene is expressed in both lytic and persistent stages of infection. This has important implications for our understanding of T-cell responses to putative latency-associated gammaherpesvirus proteins …

Anatomy Of A Youngster's Suicide: Whose Problem Is It?, Robert C. Grosz

Internet Journal of Allied Health Sciences and Practice

No abstract provided.

Skyrockets 24/7: A Response To Flat Budgets, Skyrocketing Serials Costs, And Patron Pressure For Increased Access To Electronic Journals, Cynthia Swope, Kathe Obrig, Laura E. Abate, Anne Linton

Himmelfarb Library Faculty Posters and Presentations

This poster represents the culmination of a year’s work by the Journals@Himmelfarb committee. This intra-library committee was formed to discuss the critical issues surrounding transitioning print journal subscriptions to electronic access only. In response to these discussions, collection development policies were created to reflect both print and electronic resources, a model electronic license was created, and faculty department heads were surveyed as to the usefulness and format preference for titles in their subject area. This resulted in improved understanding of the existing collection and assisted in developing the first steps for migrating from a print to an electronic collection.

Multiple Mechanisms Regulate Numa Dynamics At Spindle Poles, Olga Kisurina-Evgenieva, Gary Mack, Quansheng Du, Ian Macara, Alexey Khodjakov, Duane A. Compton

Dartmouth Scholarship

The large coiled-coil protein NuMA plays an essential role in organizing microtubule minus ends at spindle poles in vertebrate cells. Here, we use both in vivo and in vitro methods to examine NuMA dynamics at mitotic spindle poles. Using fluorescence recovery after photobleaching, we show that an exogenously expressed green-fluorescent-protein/NuMA fusion undergoes continuous exchange between soluble and spindle-associated pools in living cells. These dynamics require cellular energy and display an average half-time for fluorescence recovery of approximately 3 minutes. To explore how NuMA dynamics at spindle poles is regulated, we exploited the association of NuMA with microtubule asters formed in …

The Role Of Dairy Products In The Calcium Intake Of Suburban Adolescents, Simie Bredeweg-Dejager

Masters Theses

The purpose of this study was to examine consumption of dairy products by suburban adolescents. This was a secondary analysis of data from a questionnaire administered in late fall of 2003. There were 255 adolescents who completed the questionnaire. Data were examined to estimate calcium intake from dairy products.

There was a significant difference in the amount of calcium adolescents consume from cheese, compared to that supplied by milk (t = -8.502, p = .000). Further analyzing by gender, there was a significant difference between boys and girls intake of milk and cheese. No significant difference was found between boys …

Il-2 Receptor Signaling Regulates Ets1 Translation In Human Natural Killer Cells, Eric M. Grund

MUSC Theses and Dissertations

Natural Killer (NK) cells are critical for protection from cancer and infectious disease. The hypothesis that there is a molecular link between IL-2 receptor signaling and Ets1 was investigated. This hypothesis was based on the correlation between an NK-deficient phenotype generated by gene targeting of the Ets1 transcription factor, IL-2 receptor, IL-2 and IL-15, indicating that each of these proteins is required for the proper development and function of NK cells. The findings presented show that both IL-2 and IL-15 induce a post-transcriptional regulation of Ets1 in NK cells. Pharmacological and transient transfection analyses, using a method developed within to …

Waccnes Containing Bovine Herpe Svirus 1 Attenuated By Mutation In Latency-Related Gene, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Vaccines for pathogenic Strains of bovine herpesvirus 1 (BHV-1) which are based on attenuated BHV-1 having a mutation in the latency-related gene are provided. Live, attenuated vaccines are also provided which express anti gens from other viral or bacterial pathogens and thus form the basis of a variety of vaccines.

Bioinformatic Analysis Of A Mammalian Bip Gene For Insertion Into Green Algae And Comparison Of Its Possible Effects On The Synthesis Of A Mammalian Antibody, Katrina Ghazanfar

Theses and Dissertations

This dissertation describes a study utilizing bioinformatics to analyze homologues of a molecular chaperone, glucose-regulated protein 78 (grp 78), also known as BiP. The selected homologous proteins originate from organisms of infinitely diverse genera. Comparisons of protein sequence yielded the first clues of a common ancestry among these proteins. Furthermore, protein molecular weights, isoelectric points, N-terminal amino acids and half-lives of a known homolog and a non-homologous protein were examined. Additionally, electroporation, a state-of-the-art plasmid insertion technique, was explored using Chlamydomonas reinhardtii, a green alga, as the recipient of a parent plasmid, pSP124S. Distinctive hypertonic solutions and three separate field …

Heme Oxygenase-2 Gene Deletion Attenuates Oxidative Stress In Neurons Exposed To Extracellular Hemin., Raymond F Regan, Jing Chen, Luna Benvenisti-Zarom

Department of Emergency Medicine Faculty Papers

BACKGROUND: Hemin, the oxidized form of heme, accumulates in intracranial hematomas and is a potent oxidant. Growing evidence suggests that it contributes to delayed injury to surrounding tissue, and that this process is affected by the heme oxygenase enzymes. In a prior study, heme oxygenase-2 gene deletion increased the vulnerability of cultured cortical astrocytes to hemin. The present study tested the effect of HO-2 gene deletion on protein oxidation, reactive oxygen species formation, and cell viability after mixed cortical neuron/astrocyte cultures were incubated with neurotoxic concentrations of hemin. RESULTS: Continuous exposure of wild-type cultures to 1-10 microM hemin for 14 …

Effect Of Misreported Family History On Mendelian Mutation Prediction Models, Hormuzd A. Katki

Johns Hopkins University, Dept. of Biostatistics Working Papers

People with familial history of disease often consult with genetic counselors about their chance of carrying mutations that increase disease risk. To aid them, genetic counselors use Mendelian models that predict whether the person carries deleterious mutations based on their reported family history. Such models rely on accurate reporting of each member's diagnosis and age of diagnosis, but this information may be inaccurate. Commonly encountered errors in family history can significantly distort predictions, and thus can alter the clinical management of people undergoing counseling, screening, or genetic testing. We derive general results about the distortion in the carrier probability estimate …

Occupational Exposure To Hot Environments: Florida Workers Need Help

Florida Public Health Review

No abstract provided.

Collecting Duct Carcinoma Of The Kidney: An Immunohistochemical Study Of 11 Cases., Andrea Vecchione, Tommaso Prayer Galetti, Marina Gardiman, Hideshi Ishii, Enrico Giarnieri, Francesco Pagano, Leonard G Gomella, Carlo M Croce, Raffaele Baffa

Department of Urology Faculty Papers

BACKGROUND: Collecting duct carcinoma (CDC) is a rare but very aggressive variant of kidney carcinoma that arises from the epithelium of Bellini's ducts, in the distal portion of the nephron. In order to gain an insight into the biology of this tumor we evaluated the expression of five genes involved in the development of renal cancer (FEZ1/LZTS1, FHIT, TP53, P27kip1, and BCL2). METHODS: We studied eleven patients who underwent radical nephrectomy for primary CDC. All patients had an adequate clinical follow-up and none of them received any systemic therapy before surgery. The expression of the five markers for tumor initiation …

Oncolog, Volume 49, Number 12, December 2004, David Galloway, Katie Prout Matias, Diane Witter, Paul Mathew Md

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Advances in Prostate Cancer Treatment
• Everyday Drugs Show New Promise for Cancer
• Are COX-2 Inhibitors Safe?
• House Call: Myths & Facts about Cancer Prevention
• DiaLog: Neoadjuvant Trials in Prostate Cancer, by Paul Mathew, MD, Assistant Professor, Department of Genitourinary Medical Oncology

Amyloid Directly Inhibits Human Alpha4beta2-Nicotinic Acetylcholine Receptors Heterologously Expressed In Human Sh-Ep1 Cells, J. Wu, Y-P Kuo, L. Xu, J. B. Eaton, L. Zhao, J. Wu, R. J. Lukas

Osteopathic Medicine, Jerry M. Wallace School of

No abstract provided.

Oncolog, Volume 49, Number 11, November 2004, Rachel Williams, Steve C. Stuyck Mph

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• The Youngest Cancer Patients
• Meeting the Rising Demand for Outpatient Care
• House Call: Going Beyond the Headlines: Tips to help you evaluate cancer stories in the news media
• DiaLog: Cancer Information in the Media, by Stephen C. Stuyck, MPH, Vice President for Public Affairs

Oncolog, Volume 49, Number 10, October 2004, Dawn Chalaire, Sunni Hosemann, David Galloway, Karen Basen-Engquist Phd, Mph

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Treating Head and Neck Cancer Requires Extraordinary Coordination Among Disciplines
• Researchers Seek to Understand the Mysteries of Uterine Cancer and to Find Better Treatments
• Mammography vs. Magnetic Resonance Imaging: A Breast Cancer Prevention Specialist and a Radiologist Weigh in on the Recent Debate
• House Call: The Role of Alcohol and Tobacco in Head and Neck Cancer
• DiaLog: Sexuality after Gynecologic Cancer, by Karen Basen-Engquist, PhD, Associate Professor, Department of Behavioral Health

Catalysis Of Strand Exchange By The Hsv-1 Ul12 And Icp8 Proteins: Potent Icp8 Recombinase Activity Is Revealed Upon Resection Of Dsdna Substrate By Nuclease, Nina B. Reuven, Sandra K. Weller

UCHC Articles - Research

The replication of herpes simplex virus type 1 (HSV-1) is associated with a high degree of homologous recombination, which is likely to be mediated, in part, by HSV-1-encoded proteins. We have previously shown that the HSV-1 encoded ICP8 protein and alkaline nuclease UL12 are capable of catalyzing an in vitro strand-exchange reaction. Here, we show, by electron microscopy, that the products of the strand exchange reaction between linear double-stranded DNA and circular single-stranded DNA consist of the expected joint molecule forms: sigma, alpha, and gapped circles. Other exonucleases, such as lambda Red α, which, like UL12, digests 5′-3′, as well …

What's Happening: September, 2004, Maine Medical Center

What's Happening

No abstract provided.

Self-Help Group Participation And Empowerment In Hong Kong, Bong-Ho Mok

The Journal of Sociology & Social Welfare

This paper reports on the first comprehensive study of self-help groups in Hong Kong. Initial findings from the quantative and qualitative data suggest that self-help group participation has an impact on intrapersonal, interpersonal and community/political empowerment. Based on existing data, this study has resulted in the development of a hypothetical model encompassing the interrelationships among self-help group participation, social support, social learning, leadership and empowerment, for testing in future research.

Insulin Sensitivity Following Agent Orange Exposure In Vietnam Veterans With High Blood Levels Of 2,3,7,8-Tetrachlorodibenzo-P-Dioxin, Philip A. Kern, Sufyan Said, William G. Jackson Jr., Joel E. Michalek

Clinical and Translational Science Faculty Publications

Our objective was to determine whether insulin sensitivity was related to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Vietnam veterans exposed to Agent Orange. Air Force veterans of Operation Ranch Hand, the unit responsible for spraying Agent Orange and other herbicides in Vietnam from 1962 to 1971, and comparison veterans who did not spray herbicides were included. We measured insulin sensitivity (S_I) using a frequently sampled iv glucose tolerance test in a matched study of 29 matched pairs of veterans and a quantitative insulin sensitivity check index (QUICKI) based on fasting glucose and insulin in 71 matched pairs. No group differences were …

Cultural Value Orientations, Attributions, And Breast Cancer Screening Behaviors, Patricia M. Flynn

Loma Linda University Electronic Theses, Dissertations & Projects

Discrepancies in breast cancer screening behavior exist among various ethnic groups in the United States (Jacobs & Lauderdale, 2001), with Latino American women reporting particularly low screening rates in comparison to Anglo American women (ACS, 2002). Research indicates that behavior is in part influenced by aspects of culture and relevant psychological processes (Betancourt & Lopez, 1993; Betancourt & Fuentes, 2001). This study was designed to investigate the relations among cultural values, attributional processes, and breast cancer screening behavior among Anglo and Latino women. This study also investigated the influence of acculturation among Latino women in relation to the other study …

Psychological Factors Associated With Anticipatory Nausea And Vomiting, Melinda L. Nielsen

Loma Linda University Electronic Theses, Dissertations & Projects

Many cancer patients experience adverse chemotherapy-related side effects. The present study examined the relationships among disease variables (i.e. stage of cancer, type of breast cancer), medical treatment variables (i.e. toxicity of chemotherapy regimen, strength of antiemetic treatment), psychological variables (i.e. health locus of control, anxiety sensitivity, desire for control, coping strategies), and anticipatory nausea and vomiting in women with breast cancer. One hundred women with breast cancer completed the Multidimensional Health Locus of Control Scale - Form C, the Anxiety Sensitivity Index, the Krantz Health Opinion Survey, the Coping Inventory for Stressful Situations, and the Morrow Assessment of Nausea and …

Coping Patterns Among Perpetrators Of Child Sexual Abuse, Henry Adolphus Beck

Loma Linda University Electronic Theses, Dissertations & Projects

This study examined the coping and religious coping strategies used by child sexual abuse (CSA) perpetrators. It was hypothesized that (1) Perpetrators of incestuous CSA would show greater use of Emotion-Oriented coping than perpetrators of non-incestuous CSA, (2) perpetrators of non-incestuous CSA would show greater use of Task-Oriented coping than perpetrators of incestuous CSA (3) Perpetrators of incestuous CSA would show greater use of Avoidance-Oriented coping than perpetrators of non-incestuous CSA, and (4) there would be a difference in the use of positive and negative religious coping between groups of incestuous and non-incestuous CSA perpetrators. Participants completed the Coping Inventory …

Fisheries Research Contract Report No. 9 - Enhancing The Emergency Disease Response Capability Of The Western Australian Department Of Fisheries And Industry Bodies Associated With Freshwater Crayfish. Aquatic Animal Health Subprogram: Frdc Project No. 2003/671, Frances Stephens, Brian Jones, Iain East, Karina Scott, Simon Bennison

Fisheries Research Contract Reports

CRAYFISH PLAGUE IS A MAJOR DISEASE AFFECTING FRESHWATER CRAYFISH IN SOME AREAS OF THE WORLD. THE DISEASE HAS NEVER BEEN REPORTED IN AUSTRALIA. THE SIMULATION EXERCISE THAT WAS UNDERTAKEN AS PART OF THIS PROJECT WAS A RESPONSE TO A FICTITIOUS OUTBREAK OF CRAYFISH PLAGUE.

Funding for this project was provided to enable an exercise simulating an outbreak of crayfish plague to be conducted in Western Australia. Crayfish plague is a fungal disease (Aphanomyces astaci) that has devastated some freshwater crayfish populations in Europe but has never been recorded in Australia. The exercise was planned arounda fictitious outbreak of crayfish plague …

J Am Coll Dent 2004 71 3

Journal of the American College of Dentists

The Journal of the American College of Dentists (JACD) publishes scholarly articles, editorials, essays, and reflections addressing the ethical, professional, and social dimensions of dentistry. The Journal serves as a forum for advancing ethics, professionalism, leadership, and excellence within the dental community. By fostering dialogue across disciplines and perspectives, JACD provides readers with insights that encourage ethical, professional practice, lifelong learning, and service to the public, reinforcing dentistry’s commitment to advancing oral healthcare and the common good.

Condylar Remodeling After Distraction Osteogenesis And Bilateral Sagittal Split Osteotomy, Robert Barker Hoffman

Loma Linda University Electronic Theses, Dissertations & Projects

The present pilot study is a histological evaluation of mandibular condyles taken from minipigs that underwent either traditional mandibular advancement (bilateral sagittal split osteotomy), or a distraction osteogenesis procedure to advance the mandible. One pig received a bilateral sagittal split osteotomy with an immediate mandibular advancement of 14 mm, while another pig underwent distraction osteogenesis consisting of a gradual advancement of 2 mm per day to advance the mandible the same distance. The control animal received no surgery.

In the present study, the mandibular condyles of the three pigs (2 control, 2 bilateral sagittal split osteotomy, and 2 distraction osteogenesis) …

Levels Of Intravenous Enterococcus Faecalis That Cause Heart Colonization, Louis Zane Stromberg

Loma Linda University Electronic Theses, Dissertations & Projects

While previous studies have shown that presence of bacteria in systemic circulation can cause infective endocarditis, there is no information on the specific amount of bacteria necessary to cause this condition. The purpose of this study was to establish the minimum level of circulating bacteria that will cause colonization of damaged heart valves in rats. Fifty-two Sprague-Dawley rats were anesthetized and their heart valves were damaged using an established protocol. A clinical isolate of Enterococcus faecalis was transformed with a plasmid bearing the luxF and Chloramphenicol cassettes to label the bacteria with the light-emitting protein. After four weeks, the rats …

Jefferson Alumni Bulletin – Volume 53, Number 4, September 2004

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume 53, Number 4, September 2004 Core Competencies in the Battle against Medical Errors by Dean Thomas J. Nasca ’75; Page 4 A Promising Vaccine against Alzheimer's; Page 7 Gene Therapy That Restores Function of Damaged Human Heart Cells, in the Lab; Page 8 Clues to How Cells Migrate in Embryos; Page 8 Rosenwasser and Rosenblum Assume Key Posts; Page 10 A Hero of the French Resistance, a Jeff Alumnus; Page 11 Jefferson Specialist Is Team Physician for the U.S. Olympic Gymnastics Teams in Athens; Page 12 Healing Spirit: John Dorsey '2000; Page 20 Donald Parks …

Nf-Kappab And Ap-1 Connection: Mechanism Of Nf-Kappab-Dependent Regulation Of Ap-1 Activity, Shuichi Fujioka, Jiangong Niu, Christian Schmidt, Guido M Sclabas, Bailu Peng, Tadashi Uwagawa, Zhongkui Li, Douglas B Evans, James L Abbruzzese, Paul J Chiao

Faculty, Staff and Student Publications

Nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) transcription factors regulate many important biological and pathological processes. Activation of NF-kappaB is regulated by the inducible phosphorylation of NF-kappaB inhibitor IkappaB by IkappaB kinase. In contrast, Fos, a key component of AP-1, is primarily transcriptionally regulated by serum responsive factors (SRFs) and ternary complex factors (TCFs). Despite these different regulatory mechanisms, there is an intriguing possibility that NF-kappaB and AP-1 may modulate each other, thus expanding the scope of these two rapidly inducible transcription factors. To determine whether NF-kappaB activity is involved in the regulation of fos expression in response …

Yeast Actin Patches Are Networks Of Branched Actin Filaments, Michael E. Young, John A. Cooper, Paul C. Bridgman

Open Access Publications

No abstract provided.