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Orchestrating The Future Of Clinical Care, Brian G. Harrison, Robert L. Barchi

Thomas Jefferson University Administrative Documents and Reports

2005 Annual report of Thomas Jefferson University.

Animal Health Laboratories Service Manual 2005-2006, Department Of Agriculture And Food, Western Australia

Agriculture reports

This service manual is designed to help you, the user of services provided by the Animal Health Laboratories (AHL), to get the most from the laboratories.

The section on ‘General Instructions’ contains information required for packaging and submission of samples to AHL.

Other sections contain information on the type of sample required for each laboratory service and associated tests offered by AHL. The detail of Services offered by each laboratory is given in a table, followed by collection notes to assist with submission of specimens.

Each table describes the Services offered, the Tests available for each Service, a description of …

Jefferson Medical College Annual Report, 2005, Thomas J. Nasca

Jefferson Medical College Administrative Documents and Reports

No abstract provided.

Managing Susceptibility To Hereditary Breast And Ovarian Cancer, Cynthia E. Perry Phd, Msn, Rn

Dissertations

The recent identification of Breast Cancer 1 (BRCA1) and BRCA2 genes offers an opportunity for high-risk individuals to learn whether they may be genetically predisposed to develop breast and/or ovarian cancer. The purpose of this study was to examine how unaffected women, identified as BRCA positive and variant of uncertain significance (VUS) mutation carriers, managed their susceptibility to hereditary breast and ovarian cancer (HBOC). Thirty North American women ranging in age from 22 to 60 years responded to open-ended interviews. These interviews were analyzed using constant comparative method to generate a grounded theory. Managing Susceptibility was identified as the basic …

Effects Of Estradiol On Lipopolysaccharide And Pam3cys Stimulation Of Ccl20/Macrophage Inflammatory Protein 3 Alpha And Tumor Necrosis Factor Alpha Production By Uterine Epithelial Cells In Culture, Mardi A. Crane-Godreau, Charles R. Wira

Dartmouth Scholarship

We have previously demonstrated that rat uterine epithelial cells (UEC) produce CCL20/macrophage inflammatory protein 3 alpha (MIP3alpha) and tumor necrosis factor alpha (TNF-alpha) in response to live and heat-killed Escherichia coli and to the pathogen-associated molecular patterns (PAMP) lipopolysaccharide (LPS) and Pam3Cys. To determine whether estradiol (E2) modulates PAMP-induced CCL20/MIP3alpha and TNF-alpha secretion, primary cultures of rat UEC were incubated with E2 for 24 h and then treated with LPS or Pam3Cys or not treated for an additional 12 h. E2 inhibited the constitutive secretion of TNF-alpha and CCL20/MIP3alpha into culture media. Interestingly, E2 pretreatment enhanced CCL20/MIP3alpha secretion due to …

Mannose Binding Lectin Genotypes Influence Recovery From Hepatitis B Virus Infection, Chloe L. Thio, Timothy L. Mosbruger, Jacquie Astemborski, Spencer Greer, Gregory D. Kirk, Stephen J. O'Brien, David L. Thomas

Biology Faculty Articles

Mannose binding lectin (MBL) is a central component of the innate immune response and thus may be important for determining hepatitis B virus (HBV) persistence. Since single-nucleotide polymorphisms (SNPs) in the gene encoding MBL (mbl2) alter the level of functional MBL, we hypothesized that mbl2 genotypes are a determinant of HBV persistence or recovery from viral infection. We tested this hypothesis by using a nested case control design with 189 persons with HBV persistence matched to 338 individuals who had naturally recovered from HBV infection. We determined genotypes of two promoter and three exon 1 SNPs in mbl2 …

Thymidine-Dependent Staphylococcus Aureus Small-Colony Variants Are Associated With Extensive Alterations In Regulator And Virulence Gene Expression Profiles, Barbara C. Kahl, Gunnar Belling, Petra Becker, Indranil Chatterjee, Katrin Wardecki, Karin Hilgert, Ambrose Cheung

Dartmouth Scholarship

Chronic airway infection is a hallmark of cystic fibrosis (CF) and many CF patients are infected persistently by Staphylococcus aureus. Thymidine-dependent trimethoprim-sulfamethoxazole (SXT)-resistant S. aureus small-colony variants (SCVs), often in combination with isogenic normal S. aureus phenotypes, are highly prevalent and persistent in airway secretions of CF patients due to long-term SXT therapy (B. Kahl, M. Herrmann, A. S. Everding, H. G. Koch, K. Becker, E. Harms, R. A. Proctor, and G.

Functionally Active T1-T1 Interfaces Revealed By The Accessibility Of Intracellular Thiolate Groups In Kv4 Channels., Guangyu Wang, Mohammad Shahidullah, Carmen A Rocha, Candace Strang, Paul J Pfaffinger, Manuel Covarrubias

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Gating of voltage-dependent K(+) channels involves movements of membrane-spanning regions that control the opening of the pore. Much less is known, however, about the contributions of large intracellular channel domains to the conformational changes that underlie gating. Here, we investigated the functional role of intracellular regions in Kv4 channels by probing relevant cysteines with thiol-specific reagents. We find that reagent application to the intracellular side of inside-out patches results in time-dependent irreversible inhibition of Kv4.1 and Kv4.3 currents. In the absence or presence of Kv4-specific auxiliary subunits, mutational and electrophysiological analyses showed that none of the 14 intracellular cysteines is …

Calibration And Evaluation Of An Objective Measure Of Physical Activity In Preschool Children, John R. Sirard, Stewart G. Trost, Karin A. Pfeiffer, Marsha Dowda, Russell R. Pate

Faculty Publications

Background: The purposes of this study were 1) to establish accelerometer count cutoffs to categorize activity intensity of 3 to 5-y old-children and 2) to evaluate the accelerometer as a measure of children's physical activity in preschool settings. Methods: While wearing an ActiGraph accelerometer, 16 preschool children performed five, 3-min structured activities. Receiver Operating Characteristics (ROC) curve analyses identified count cutoffs for four physical activity intensities. In 9 preschools, 281 children wore an ActiGraph during observations performed by three trained observers (interobserver reliability = 0.91 to 0.98). Results: Separate count cutoffs for 3, 4, and 5-y olds were established. Sensitivity …

Erythroid Cell-Specific Α-Globin Gene Regulation By The Cp2 Transcription Factor Family, Ho C. Kang, Jui Hyung Chae, Yeon H. Lee, Mi-Ae Park, June Ho Shin, Sung-Hyun Kim, Sang-Kyu Ye, Yoon Shin Cho, Steven Fiering, Chul Geun Kim

Dartmouth Scholarship

We previously demonstrated that ubiquitously expressed CP2c exerts potent erythroid-specific transactivation of alpha-globin through an unknown mechanism. This mechanism is reported here to involve specific CP2 splice variants and protein inhibitor of activated STAT1 (PIAS1). We identify a novel murine splice isoform of CP2, CP2b, which is identical to CP2a except that it has an additional 36 amino acids encoded by an extra exon. CP2b has an erythroid cell-specific transcriptional activation domain, which requires the extra exon and can form heteromeric complexes with other CP2 isoforms, but lacks the DNA binding activity found in CP2a and CP2c. Transcriptional activation of …

A Human T-Cell Lymphotropic Virus Type 1 Enhancer Of Myc Transforming Potential Stabilizes Myc-Tip60 Transcriptional Interactions, Soumya Awasthi, Anima Sharma, Kasuen Wong, Junyu Zhang, Elizabeth F. Matlock, Lowery Rogers, Pamela Motloch, Shigeki Takemoto, Hirokuni Taguchi, Michael D. Cole

Dartmouth Scholarship

The human T-cell lymphotropic virus type 1 (HTLV-1) infects and transforms CD4+ lymphocytes and causes adult T-cell leukemia/lymphoma (ATLL), an aggressive lymphoproliferative disease that is often fatal. Here, we demonstrate that the HTLV-1 pX splice-variant p30II markedly enhances the transforming potential of Myc and transcriptionally activates the human cyclin D2 promoter, dependent upon its conserved Myc-responsive E-box enhancer elements, which are associated with increased S-phase entry and multinucleation. Enhancement of c-Myc transforming activity by HTLV-1 p30II is dependent upon the transcriptional coactivators, transforming transcriptional activator protein/p434 and TIP60, and it requires TIP60 histone acetyltransferase (HAT) activity and correlates with the …

Prevention Of Periodontal Diseases, Andrew R. Dentino, Moawia M. Kassab, Erica J. Renner

School of Dentistry Faculty Research and Publications

No abstract provided.

Jefferson Cancer Center Officially Recognized The National Cancer Institute, Jerold A. Glick

Population Health Matters (Formerly Health Policy Newsletter)

No abstract available.

Biotechnology Program Prepares Future Researchers, Lydia E. Mcmorrow, Shirley E. Greening

Population Health Matters (Formerly Health Policy Newsletter)

No abstract available.

Diets And Coexistence Of The Sea Urchins Lytechinus Variegatus And Arbacia Punctulata (Echinodermata) Along The Central Florida Gulf Coast, Janessa Cobb, John M. Lawrence

Integrative Biology Faculty and Staff Publications

The basis for coexistence of similar species is fundamental in community ecology. One mechanism for coexistence is differentiation of diets. Lytechinus variegatus and Arbacia punctulata coexist in different microhabitats along the Florida gulf coast. Their great difference in morphology might affect their choice of microhabitats and diet. We analyzed diets of both species at 1 offshore and 1 nearshore site where both occurred in relatively equal numbers, an offshore site dominated by A. punctulata and an offshore site dominated by L. variegatus. Gut contents were analyzed to determine the diet. A. punctulata prim. consumed sessile invertebrates except on dates …

Ada News - 06/20/2005, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Evaluating Forensic Dna Evidence, Dan E. Krane, William C. Thompson

Biological Sciences Faculty Publications

No abstract provided.

Research News: 2005, No. 2, University Of Mississippi. School Of Pharmacy

Research News: Grants and Publications (2000-2014)

Grants, publications, technology transfer, special announcements

Urotensin Ii Modulates Rapid Eye Movement Sleep Through Activation Of Brainstem Cholinergic Neurons, Salvador Huitron-Resendiz, Morten P. Kristensen, Stephen L. Grupke, Christopher Tyler, Olivier Civelli, Christopher S. Leonard, Luis De Lecea

NYMC Faculty Publications

Urotensin II (UII) is a cyclic neuropeptide with strong vasoconstrictive activity in the peripheral vasculature. UII receptor mRNA is also expressed in the CNS, in particular in cholinergic neurons located in the mesopontine tegmental area, including the pedunculopontine tegmental (PPT) and lateral dorsal tegmental nuclei. This distribution suggests that the UII system is involved in functions regulated by acetylcholine, such as the sleep-wake cycle. Here, we tested the hypothesis that UII influences cholinergic PPT neuron activity and alters rapid eye movement (REM) sleep patterns in rats. Local administration of UII into the PPT nucleus increases REM sleep without inducing changes …

Gonadal Steroids Differentially Modulate Neurotoxicity Of Hiv And Cocaine: Testosterone And Ici 182,780 Sensitive Mechanism, Sherie L. Kendall, Caroline F. Anderson, Avindra Nath, Jadwiga Turchan-Cholewo, Cantey L. Land, Charles F. Mactutus, Rosemarie M. Booze

Behavioral Science Faculty Publications

BACKGROUND: HIV Associated Dementia (HAD) is a common complication of human immunodeficiency virus (HIV) infection that erodes the quality of life for patients and burdens health care providers. Intravenous drug use is a major route of HIV transmission, and drug use is associated with increased HAD. Specific proteins released as a consequence of HIV infection (e.g., gp120, the HIV envelope protein and Tat, the nuclear transactivating protein) have been implicated in the pathogenesis of HAD. In primary cultures of human fetal brain tissue, subtoxic doses of gp120 and Tat are capable of interacting with a physiologically relevant dose of cocaine, …

The Effects Of Price Regulation On Pharmaceutical R&D And Innovation, Heather M. O'Neill, Lena Clarissa Crain

Business and Economics Faculty Publications

As rising health care expenditures focus government attention on slowing the growth, the pharmaceutical industry comes under increasing pressure to curb prices of ethical drugs. Pharmaceutical price regulations have been implemented in many countries to control pharmaceutical expenditures. Yet, creating innovative drugs requires enormous R&D costs, which in turn require adequate expected economic returns. Since price controls reduce profits and expected returns, as countries invoke stricter price regulations, firms will either move their R&D process into less regulated markets or move out of innovative R&D. This paper assesses the impact of drug price regulations in Japan compared to market-priced drugs …

Dentalgram - Vol. 19, No. 06, Loma Linda University School Of Dentistry

Dentalgram

In this issue:

• Congratulations to CDA/CDHA winners and participants!
• Graduate periodontic students present research
• 20th Annual Children's Day fair held May 17 at LLU campus mall
• Students receive numerous awards and honors
• Pierre Fauchard Academy honors LLUSD faculty member, alumnus, and students
• In memoriam - Dan M. Nakamura, DDS, PG'81
• Dr. Charles J. Goodacre receives the 2005 George Moulton Award
• Commencement address: Three words
• Dr. Michael Fitzpatrick named School of Dentistry Teacher of the Year
• Department news
• Student attend dental meeting in Chicago
• International Dentist Program banquet held May 25
• Up close and personal: The life of Harold Schnepper

A Critical Role For Kalirin In Ngf Signaling Through Trka, Kausik Chakrabarti, Rong Lin, Noraisha I. Schiller, Yanping Wang, David Koubi, Ying-Xin Fan, Brian B. Rudkin, Gibbes R. Johnson, Martin R. Schiller

Life Sciences Faculty Research

Kalirin is a multidomain guanine nucleotide exchange factor (GEF) that activates Rho proteins, inducing cytoskeletal rearrangement in neurons. Although much is known about the effects of Kalirin on Rho GTPases and neuronal morphology, little is known about the association of Kalirin with the receptor/signaling systems that affect neuronal morphology. Our experiments demonstrate that Kalirin binds to and colocalizes with the TrkA neurotrophin receptor in neurons. In PC12 cells, inhibition of Kalirin expression using antisense RNA decreased nerve growth factor (NGF)-induced TrkA autophosphorylation and process extension. Kalirin overexpression potentiated neurotrophin-stimulated TrkA autophosphorylation and neurite outgrowth in PC12 cells at a low …

Antibody Responses In Reindeer (Rangifer Tarandus) Infected With Mycobacterium Bovis, W. R. Waters, M. V. Palmer, J. P. Bannantine, R. Greenwald, J. Esfandiari, P. Andersen, J. Mcnair, J. M. Pollock, K. P. Lyashchenko

Michigan Bovine Tuberculosis Bibliography and Database

Despite having a very low incidence of disease, reindeer (Rangifer tarandus) are subject to tuberculosis (TB) testing requirements for interstate shipment and herd accreditation in the United States. Improved TB tests are desperately needed, as many reindeer are falsely classified as reactors by current testing procedures. Sera collected sequentially from 11 (experimentally) Mycobacterium bovis-infected reindeer and 4 noninfected reindeer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for antibody specific to M. bovis antigens. Specific antibody was detected as early as 4 weeks after challenge with M. bovis. By MAPIA, sera …

Rural Methamphetamine Abuse: An Ethnographic Perspective, Rocky L. Sexton, Robert G. Carlson

Population and Public Health Sciences Faculty Publications

No abstract provided.

Stimulant Abuse In Ohio And The Rural South, Robert G. Carlson

Population and Public Health Sciences Faculty Publications

This paper summarizes the methods and findings from two NIDA-supported studies of methamphetamine (MA) abuse in rural Ohio, Kentucky, and Arkansas, conducted by researchers at the Wright State University Boonshoft School of Medicine, the University of Arkansas for Medical Sciences, and the University of Kentucky. In addition, patterns of MA abuse among young adult MDMA/ecstasy users in urban central Ohio are described. Finally, preliminary findings from the Ohio Substance Abuse Monitoring Network on trends in methamphetamine abuse in Dayton, Ohio, are presented.

Resveratrol Regulates Igf- Ii And Cathepsin D In Breast Cancer Cells, Sharda Kalla Vyas

Loma Linda University Electronic Theses, Dissertations & Projects

Breast cancer is the most common type of cancer among women in the US. Phytoestrogen resveratrol (RS V) has been described as a potential ehemotherapeutic agent, but has been associated with breast cancer cell growth and cell death. RSV binds to estrogen receptors (ER) and functions as a mixed estrogen agonist/antagonist. The mechanism by which RSV regulates breast cancer growth is largely unknown, but it likely targets factors regulated by estrogens. Insulin-like growth factor II (IGF-II) and its precursor (proIGF-II) are estrogen-regulated mitogens that are over-expressed in breast carcinoma. Thus, I hypothesized that IGF-II/proIGF-II might mediate the biphasic effects of …

Manganese Distribution Across The Blood-Brain Barrier. Iv. Evidence For Brain Influx Through Store-Operated Calcium Channels, Janelle S. Crossgrove, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Manganese (Mn) is a required co-factor for many ubiquitous enzymes; however, chronic Mn overexposure can cause manganism, a parkinsonian-like syndrome. Previous studies showed Mn influx into brain is carrier-mediated, though the putative carrier(s) were not established. Studies conducted with cultured bovine brain microvascular endothelial cells (bBMECs), which comprise the blood–brain barrier, revealed ⁵⁴Mn (II) uptake positively correlated with pH, was temperature-dependent, and was sodium- and energy-independent. Brain ⁵⁴Mn uptake correlated inversely with calcium (Ca) concentration, but ⁴⁵Ca uptake was unaltered by high Mn concentration. Lanthanum (La), a non-selective inhibitor of several Ca channel types, as well as …

Religiosity And Prosocial Behaviours In Adolescence: The Mediating Role Of Prosocial Values, Sam A. Hardy, Gustavo Carlo

Department of Psychology: Faculty Publications

This study examined the hypothesis that religiosity would be differentially related to six types of adolescent prosocial behavior, and that these relations would be mediated by the prosocial value of kindness. Self-report data were collected from 142 high school students (63 per cent female; 91 per cent White; M age = 16.8, S =.80). Religiosity was a significant positive predictor of kindness, as well as compliant, anonymous and altruistic prosocial behavior, but not public, dire and emotional prosocial behavior. Associations between religiosity and both compliant and altruistic prosocial behaviors were mediated by kindness. Direct and indirect paths were found between …

The Ecology And Evolution Of Patience In Two New World Monkeys, Jeffrey R. Stevens, Elizabeth V. Hallinan, Marc D. Hauser

Department of Psychology: Faculty Publications

Decision making often involves choosing between small, short-term rewards and large, long-term rewards. All animals, humans included, discount future rewards—the present value of delayed rewards is viewed as less than the value of immediate rewards. Despite its ubiquity, there exists considerable but unexplained variation between species in their capacity to wait for rewards—that is, to exert patience or self-control. Using two closely related primates—common marmosets (Callithrix jacchus) and cotton-top tamarins (Saguinus oedipus)— we uncover a variable that may explain differences in how species discount future rewards. Both species faced a self-control paradigm in which individuals chose …