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Clinicopathological Features Of Colorectal Cancer In Young Patients In Comparison With Older Patients, Bahaa Al-Neama, Wasam Al-Asadi

Iraqi National Journal of Medicine

Background:  Colorectal cancer (CRC) has traditionally been considered a disease of older adults; however, recent evidence shows an increasing incidence among younger patients. Understanding the differences in clinicopathological features between young and older individuals is essential for improving early diagnosis, treatment strategies, and outcomes. Aim: The primary objective of this study is to compare the demographic profiles and clinicopathological characteristics of younger patients (aged <40 years) with those of older patients (aged ≥40 years) diagnosed with colorectal cancer. Methods: Eighty-five consecutive patients admitted to our hospital were prospectively followed for their entire hospital stay or up to 7 days, whichever came first. Each patient's blood glucose measurement was recorded according to ward protocol. Indices included total blood glucose per ward, per patient-day, or patient stay, and percentage blood glucose levels per predefined cut points to assess the incidence of hypo-, eu-, and hyperglycemia. The trial registration number at Clinicaltrials.org is NCT04800861. Results: Total data consisted of 645 blood glucose tests from 85 patients and 284 patient-day results, with an average of 2.27 tests per patient-day and 7.59 measurements per patient stay. The percentage of blood glucose in the range (80–180 mg/dL) varied by model, with patient-day showing the highest results at 48.24%, while the percentage of patients with hypoglycemia (blood glucose <60 mg/dL) was highest per patient stay at 1.18%. Conclusions: Younger colorectal cancer patients in this study presented more frequently with rectal tumors, poor differentiation, and advanced disease stages. Despite similar symptom profiles, delayed diagnosis may contribute to worse pathological features. These findings support the need for greater awareness and consideration of earlier screening in younger populations.

Aberrant Cd25 And Increased Cd123 Expression Are Common In Acute Myeloid Leukemia With Kmt2a Partial Tandem Duplication And Are Associated With Flt3 Internal Tandem Duplication, Qing Wei, Guilin Tang, Shaoying Li, Sa A Wang, Pei Lin, Wei Wang, Sanam Loghavi, Wei J Wang, L Jeffrey Medeiros, Jie Xu

Faculty, Staff and Student Publications

Background: KMT2A partial tandem duplication (PTD) occurs in approximately 5-10% of acute myeloid leukemia (AML) cases and is associated with poor prognosis. While its cytogenetic and molecular features are well described, the immunophenotypic characteristics of AML with KMT2A-PTD remain incompletely defined.

Methods: We identified 47 cases of AML with KMT2A-PTD by optical genome mapping. All cases underwent flow cytometric immunophenotypic analysis and next-generation sequencing using an 81-gene panel.

Results: The cohort included 32 men and 15 women with a median age of 67 years (range, 19-87). Thirty-eight cases were de novo AML, and nine were secondary to myelodysplastic …

Emt-Induced Stem Cell And Mesenchymal Programs Can Be Decoupled Via Cell Division And Esrp1-Dependent Mechanisms, Petra Den Hollander, Maria Castaneda, Suhas V Vasaikar, Joanna Joyce Maddela, Claire Gould, Breanna R Demestichas, Robiya Joseph, Shivangi Agarwal, Abhijeet P Deshmukh, Alvina Zia, Shruti Shah, Tieling Zhou, Geraldine Raja, Paul Allegakoen, Nick A Kuburich, Mika Pietila, Chunxiao Fu, Jeffrey Chang, Chad J Creighton, William F Symmans, Rama Soundararajan, Sendurai A Mani

Faculty, Staff and Student Publications

Epithelial-to-mesenchymal transition (EMT) is known to induce both stemness and mesenchymal properties, and our findings reveal that these two programs can be uncoupled. During EMT, epithelial cells transition from symmetric divisions producing differentiated daughter cells to self-renewing daughter cells. When we block cell division and induce EMT, cells gain mesenchymal properties but not stemness, suggesting the importance of cell division for gaining stemness. We identified ESRP1 as a key regulator of EMT-driven stemness, which get downregulated during EMT in a cell division-dependent manner. Overexpression of ESRP1 prevents the gain of stemness without affecting the mesenchymal program. Only the stemness and …

Role Of The Etv5/P38 Signaling Axis In Aggressive Thyroid Cancer Cells, Jerry H Houl, Rozita Bagheri-Yarmand, Muthusamy Kunnimalaiyaan, Paola Miranda Mendez, Joseph L Kidd, Ali Dadbin, Andrea Ruiz-Jurado, Parag A Parekh, Ying C Henderson, Nikhil S Chari, Aatish Thennavan, Reid T Powell, Clifford C Stephan, Xiao Zhao, Anastasios Maniakas, Roza Nurieva, Naifa L Busaidy, Maria E Cabanillas, Ramona Dadu, Mark Zafereo, Jennifer R Wang, Stephen Y Lai, Marie-Claude Hofmann

Faculty, Staff and Student Publications

Patients with poorly differentiated thyroid cancer (PDTC) and anaplastic thyroid cancer (ATC) face a much poorer prognosis than those with differentiated thyroid cancers. Around 25% of PDTCs and 35% of ATCs carry the BRAFV600E mutation, which constitutively activates the MAPK pathway, a key driver of cell growth. Although combining BRAF and MEK inhibitors can shrink tumors, resistance often develops. The exact cause of this resistance remains unclear. We previously found that in PDTC and ATC cells, the BRAFV600E mutation is strongly linked to the expression of ETV5, a transcription factor downstream of the MAPK pathway. In the current study, we …

Advances In Targeting Her2 Across Cancer Subtypes: A Pan-Tumor Approach, Taiwo Adesoye, Ecaterina E Dumbrava, Kanwal P S Raghav, Aysegul A Sahin, Hui Chen, Sunyoung S Lee, Milind M Javle, Shubham Pant, Omar Alhalabi, Xiuning Le, Vicente Valero, Paula R Pohlmann, Funda Meric-Bernstam

Faculty, Staff and Student Publications

Human epidermal growth factor receptor 2 (HER2) is an established therapeutic target in multiple solid tumors, particularly breast and gastric cancers. Significant advancements have been made in the development of HER2-targeted therapies, including monoclonal antibodies, tyrosine kinase inhibitors, antibody-drug conjugates (ADC), and novel bispecific antibodies. These agents have revolutionized the treatment landscape for HER2-positive metastatic cancers, resulting in improved progression-free and overall survival, and quality of life for patients. Beyond breast and gastric cancers, HER2 expression/amplification has been observed in other solid tumors, such as colorectal, lung, bladder, ovarian, and biliary tract cancers, offering new opportunities for personalized therapy in …

Selective Deletion Of Slc2a1 From The Rpe Reveals That Rods But Not Cones Depend On Glucose Transport Across The Outer Blood-Retinal Barrier, Lauren L. Daniele, John Y.S. Han, Minzhong Yu, Ravi A. Sangani, Craig D. Beight, Cyrus Rostami, Philip D. Kiser, Neal S. Peachy, Nancy J. Philp

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

GLUT1 facilitates a continuous flow of glucose across the inner and outer blood-retinal barriers (BRBs) to support vision. To understand the extent to which photoreceptors rely on glucose transport across the outer BRB, we generated a tamoxifen-inducible conditional knockout of Slc2a1 in the retinal pigment epithelium (RPE) (RPE-iΔGlut1). In the RPE-iΔGlut1 mice, rod photoreceptors exhibited impaired outer segment renewal and decreased the expression of proteins involved in phototransduction and ciliary transport. Proteins regulating the retinal stress response increased. Cone photoreceptors were functional and viable 15 months post-tamoxifen treatment in the RPE-iΔGlut1 mice, while 70% of the rods died. …

Optimal Murine Cd4+ T Cell Priming By Mrna-Lipid Nanoparticle Vaccines Requires Endogenous Antigen Processing, Julia E. Rood, Suh Kyung Yoon, Mary K. Heard, Stephen D. Carro, Emma J. Hedgepeth, Mary E. O'Mara, Michael J. Hogan, Nhu Le, Hiromi Muramatsu, Kieu Lam, Petra Schreiner, Coral Kasden, Hansell H. Stedman, Ryan A. Langlois, James Heyes, Norbert Pardi, Laurence C. Eisenlohr

College of Life Sciences Faculty Papers

Lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA vaccines elicit robust CD4+ T cell responses, yet the mechanisms underlying this T cell priming remain unknown. Antigens presented to CD4+ T cells on major histocompatibility complex class II (MHC II) are traditionally acquired by antigen presenting cells (APCs) from extracellular sources. Here we show that vaccine specific CD4+ T cell responses instead rely on antigen directly expressed within APCs, without extracellular transit. Murine APCs treated with mRNA-LNP vaccines activate T cells more efficiently when presenting antigen produced internally, rather than acquired externally. Immunization with mRNA-LNP vaccines engineered to inhibit antigen expression in APCs results …

Single-Cell Rna Sequencing Suggests Different Progenitor Lineages Between Idh Mutant And Idhwt Glioma, Iyad Alnahhas, Allison Kayne, Mehak Khan, Wenyin Shi

Department of Neurology Faculty Papers

Introduction Single-cell RNA sequencing has elucidated the heterogeneity in cancer. Single-cell glioblastoma (GBM) analyses have also proposed the resemblance of GBM cells to radial glia and outer radial glia (oRG) supporting the hypothesis that remnants of developmental tissue get reactivated in cancer. A recent study isolated neural progenitor cells (NPCs) from developing fetal human brain (gestational week 17-19) and classified NPCs based on their expression of THY1 (CD90), CD24 and EGFR. Ventricular radial glia are THY1⁻CD24⁻EGFR⁺ whereas oRG are THY1⁻CD24⁻EGFR⁻. Early neuron precursors are CD24⁺THY1⁻EGFR …

The Ethical Limits Of Physician Autonomy In Reproductive Medicine, Isabel Martens

Honors Theses

In vitro fertilization is a relatively new medical practice that helps people who face infertility be able to reproduce. Reproductive medicine providers face ethical dilemmas often in their field, only some of which have standards of care. There are questions in the medical landscape pertaining to physician autonomy versus patient autonomy, which heavily apply to reproductive medicine providers. Patient autonomy is highly valued in medical ethics; however, physician autonomy should also be considered. When a physician decides who can and cannot reproduce, ethical considerations must be followed to maintain the practice of reproductive autonomy. Limited policies exist that manage reproductive …

Combining Dna Methylation Features And Clinical Characteristics Predicts Ketamine Treatment Response For Ptsd, Amir Valizadeh, John D Roache, Xinyu Zhang, Ying Hu, Ralitza Gueorguieva, Lynnette A Averill, Mohini Ranganathan, Zuoheng Wang, Douglas E Williamson, Paulo R Shiroma, Matthew J Girgenti, Ismene L Petrakis, Argelio L López-Roca, Stacey Young-Mccaughan, Terence M Keane, Alan L Peterson, Chadi G Abdallah, John H Krystal, Ke Xu

Staff and Researcher Publications

Post-traumatic stress disorder (PTSD) exhibits extensive clinical and biological variability, making treatment challenging. The Consortium to Alleviate PTSD (CAP)-ketamine trial, the largest randomized study of ketamine for PTSD, found no overall benefit of ketamine over placebo, underscoring the necessity to identify responsive subgroups. Using pre-treatment blood DNA methylation profiles and clinical measures from the CAP-ketamine trial, we applied machine learning to predict treatment response. A model based on 1,208 methylation sites achieved higher predictive accuracy than models using clinical variables alone, and combining both data types further improved performance. The methylation-derived score distinguished responders with 92.9% accuracy. The predictive CpGs …

Rare Heterozygous De Novo Variants In Rapgef2 Are Associated With A Neurodevelopmental Disorder, Ali H Bereshneh, Kirkland A Wilson, Xueyang Pan, Shabab B Hannan, Megan A Cooper, Jullianne Diaz, Eyby Leon, Tiana M Moses, Mahshid S Azamian, Daryl A Scott, Ping Yee Billie Au, Juan Pablo Appendino, Ingrid E Scheffer, Antony Kaspi, Melanie Bahlo, Michael S Hildebrand, Angela T Morgan, Ekanem Ekure, Joshua M Shulman, Friedhelm Hildebrandt, Jennifer E Posey, Paul Kruszka, Eric Vilain, Shinya Yamamoto, Oguz Kanca, Seth Berger, Hugo J Bellen

Duncan NRI Faculty and Staff Publications

Purpose: RAPGEF2 encodes a guanine nucleotide exchange factor (GEF) that activates small GTPases and has not been linked to a Mendelian disorder. RAPGEF2 is highly intolerant to loss-of-function variants. We report 5 de novo heterozygous variants in RAPGEF2 in unrelated individuals with developmental delay, attention deficit hyperactivity disorder, epilepsy, dysmorphic features, or other manifestations. We used a Drosophila model to assess the functional impact of the identified human variants.

Methods: We generated a Kozak-GAL4 null allele of the Drosophila ortholog of RAPGEF2, PDZ-GEF, and used the allele to determine the gene expression pattern as well as the loss-of-function phenotypes. We …

Ruth Coleman Denard (1906-2002): One Of The First Registered Black Occupational Therapists, Mirline Milien

The Open Journal of Occupational Therapy

The experiences and contributions of underrepresented groups in the occupational therapy profession are insufficiently explored and largely absent from occupational therapy education. For instance, the narratives of early Black occupational therapists and students are virtually nonexistent in the literature. As the profession moves toward greater inclusivity, it is essential to uncover these hidden stories and legacies to better understand the profession’s evolution and help shape its future. This article highlights Ruth Coleman Denard, one of the first registered Black occupational therapists in the United States, and provides an overview of her career trajectory. Historical research methods, including a review of …

Enhancing Self-Esteem In Adolescents During Acute Inpatient Psychiatric Hospitalization: A Mixed Methods Study, Praveen Injeti, Julie D. Kugel, Dragana Krpalek, Liane Hewitt, Lida Gharibvand

The Open Journal of Occupational Therapy

Background: Low self-esteem (LSE) can negatively impact adolescent mental health, leading to suicidal ideation (SI). Social stressors, such as peer rejections, are considered a source of stress that can contribute to LSE, SI, and subsequent admission to psychiatric facilities. This study aimed to assess the effectiveness of an occupational therapy intervention to improve self-awareness and self-esteem during short-term hospitalization.

Method: The research used a mixed methods approach to evaluate the impact of a psychoeducational intervention by an occupational therapist on 16 adolescents. The Rosenberg Self-esteem Scale (RSES) was used before the intervention (pretest), immediately after the intervention (posttest …

State Of The Journal: Advancing And Promoting Frames Of Reference For Occupational Therapy Practice, Diane Powers Dirette

The Open Journal of Occupational Therapy

No abstract provided.

Whole Genome Sequence Analysis Of Pulmonary Function And Copd In 44,287 Multi-Ancestry Participants, Wonji Kim, Xiaowei Hu, Kangjin Kim, Sung Chun, Peter Orchard, Dandi Qiao, Ingo Ruczinski, Aabida Saferali, Francois Aguet, Lucinda Antonacci-Fulton, Pallavi P Balte, Traci M Bartz, Wardatul Jannat Anamika, Xiaobo Zhou, Junyi Duan, Jennifer A Brody, Brian E Cade, Martha L Daviglus, Harshavadran Doddapaneni, Shannon Dugan-Perez, Susan K Dutcher, Christian D Frazar, Stacey B Gabriel, Sina A Gharib, Namrata Gupta, Brian D Hobbs, Silva Kasela, Laura R Loehr, Ginger A Metcalf, Donna M Muzny, Elizabeth C Oelsner, Laura J Rasmussen-Torvik, Colleen M Sitlani, Joshua Smith, Tamar Sofer, Hanfei Xu, Bing Yu, David Zhang, John Ziniti, R Graham Barr, April P Carson, Myriam Fornage, Lifang Hou, Ravi Kalhan, Robert Kaplan, Tuuli Lappalainen, Stephanie J London, Alanna C Morrison, George T O'Connor, Bruce M Psaty, Laura M Raffield, Susan Redline, Stephen S Rich, Jerome I Rotter, Edwin K Silverman, Ani Manichaikul, Michael H Cho

Faculty, Staff and Student Publications

Background: Whole genome sequence (WGS) data in multi-ancestry samples supports discovery of low-frequency or population-specific genetic variants associated with chronic obstructive pulmonary disease (COPD) and lung function.

Results: We performed single variant, structural variant, and gene-based analysis of pulmonary function (FEV1, FVC and FEV1/FVC) and COPD case-control status in 44,287 multi-ancestry participants from the NHLBI Trans-Omics for Precision Medicine (TOPMed) Program. We validated findings using the UK Biobank and assessed implicated genes using lung single-cell RNA-seq (scRNA-seq) data sets. Applying a genome-wide significance threshold (P < 5 × 10-9), we replicated known loci and identified novel associations near LY86, MAGI1, GRK7, and LINC02668. Colocalization with gene expression quantitative trait loci (eQTL) from the Lung Tissue Research Consortium highlighted known candidate genes including ADAM19, THSD4, C4B, and PSMA4, which were not identified through other eQTL sources. Multi-ancestry analysis improved fine-mapping resolution (e.g., HTR4 and RIN3). Gene-based analysis identified and replicated HMCN1. In human lung scRNA-seq data sets, lung epithelial cells and immune cell types showed enriched expression, while fibroblasts showed higher expression for HMCN1. CRISPR targeting HMCN1 in IMR90 demonstrated reduced expression of collagen genes.

Conclusions: Large-scale multi-ancestry WGS analysis improves variant discovery and fine-mapping resolution for lung function and …

Teriparatide Use In Osteopenic Patients Undergoing Single-Level Lumbar Fusion Associated With Decreased 2-Year Revision Rates, Mitchell K. Ng, Ariel N. Rodriguez, Abigail Razi, Ahmed K. Emara, Brian T. Ford, Ameer Tabbaa, Divya Gouni, Jacquelyn J. Xu, Paul G. Mastrokostas, Jad Bou Monsef, Afshin E. Razi

Rothman Institute Papers

Context:  Low bone density is common among lumbar fusion patients and increases the risks of pseudarthrosis, hardware failure, and revision. Teriparatide improves bone mass and fusion in osteoporosis, but its benefit in osteopenia or normal bone remains uncertain.

Aims:  To evaluate the association between teriparatide exposure and (1) postoperative complications, (2) 90-day readmission, and (3) 2-year reoperation, stratified by bone health status (osteoporotic, osteopenic, and normal) in patients undergoing single-level lumbar fusion.

Setting and Design:  A retrospective cohort study using the PearlDiver database from 2010 to 2021 identified patients undergoing single-level lumbar fusion.

Subjects and Methods:  Patients on teriparatide were …

The Impact Of Vdr Gene And Biochemical Indicators In Osteoporosis, Sabah Subhi Ismael Barani, Mohammed Abdullah Ajeel, Safwan Jasim Sultan

BioMed Visions Journal

Osteoporosis is a disorder caused by bone remodeling imbalance in which bone resorption outpaces bone production, resulting in weaker bone structure. Factors such as heredity and insufficient calcium and vitamin D consumption.

This study included an examination of 80 female patients of different ages (40–58 years) in Mosul. The participants were divided into two groups: 20 individuals served as the control group, while the rest 60 were osteoporotic women based on clinical cases and taking X ray DEXA. The blood samples are divided into two parts serum for biochemical tests and blood for genetic diversity in genes associated to Vitamin …

Sirtuins And Cartilage Preservation: From Cellular Function To Therapeutic Innovation, Muthanna K. Zaki, Khalil A. Hadid, Mohammed N. Abed, Fawaz A. Alassaf

BioMed Visions Journal

The bones are cushioned by a thin layer of hyaline cartilage that permits physical activity to be performed without experiencing any discomfort. However, these tissues are susceptible to several diseases involving osteoarthritis and rheumatoid arthritis resulting in cartilage degradation. Sirtuins (SRTs) and SRT-activating molecules have been shown in previous studies to affect cartilage and chondrocytes positively. This review aims to explore the potential protective effects of SRTs on cartilage and chondrocytes. It also highlighting various SRT-activating substances and their potential therapeutic applications in managing cartilage-related diseases. Google Scholar, Cochrane Library, and PubMed were used to search about related studies. The …

Whole Genome Sequence Analysis Of Pulmonary Function And Copd In 44,287 Multi-Ancestry Participants, Wonji Kim, Lucinda Antonacci-Fulton, Susan K Dutcher, Et Al.

2020-Current year OA Pubs

BACKGROUND: Whole genome sequence (WGS) data in multi-ancestry samples supports discovery of low-frequency or population-specific genetic variants associated with chronic obstructive pulmonary disease (COPD) and lung function.

RESULTS: We performed single variant, structural variant, and gene-based analysis of pulmonary function (FEV

CONCLUSIONS: Large-scale multi-ancestry WGS analysis improves variant discovery and fine-mapping resolution for lung function and COPD and highlights biologically relevant genes and pathways.

Anoikis Resistance And Metastasis Of Ovarian Cancer Can Be Overcome By Cdk8/19 Mediator Kinase Inhibition, Mehri Monavarian, Resha Rajkarnikar, Emily Faith Page, Asha Kumari, Liz Quintero Macias, Felipe Massicano, Nam. Y. Lee, Sarthak Sahoo, Nadine Hempel, Mohit Kumar Jolly, Lara Ianov, Elizabeth Worthey, Abhyudai Singh, Igor Roninson Ph.D., Eugenia Broude Ph.D., Mengqian Chen Ph.D., Karthikeyan Mythreye

Faculty Publications

Anoikis resistance, or evasion of cell death triggered by matrix detachment, is a hallmark of cancer cell survival and metastasis. We showed that repeated exposure to suspension stress followed by recovery under attached conditions leads to development of anoikis resistance. The acquisition of anoikis resistance was associated with enhanced invasion, chemoresistance, and immune evasion in vitro and distant metastasis in vivo. This acquired anoikis resistance was not genetic, persisting for a finite duration without detachment stress, but was sensitive to CDK8/19 mediator kinase inhibition that could also reverse anoikis resistance. Transcriptomic analysis revealed that CDK8/19 kinase inhibition induces bidirectional transcriptional …

Rab5c Increases Endothelial Release Of Vwf By Regulating Vesicle Trafficking, Paula Reventun, Pablo Toledano-Sanz, Maria Delgado-Marin, Maria Viskadourou, D Brian Foster, Paul S De Vries, Maria Sabater-Lleal, Nunzio Alcharani, Claudia Gonzalez-Cucharero, William O Osburn, Alanna C Morrison, Alisa S Wolberg, Nicholas L Smith, Marios Arvanitis, Charles J Lowenstein

Faculty, Staff and Student Publications

Background: Abnormal levels of VWF (von Willebrand Factor) are a risk factor for venous thromboembolism (VTE) and bleeding. Genome-wide association studies for VWF have identified novel candidate genes that may regulate VWF levels in humans, including RAB5C (RAS-associated protein RAB5C). We hypothesized that RAB5C regulates VWF release from endothelial cells.

Methods: We studied the effect of RAB5C on vesicle trafficking in human endothelial cells. We performed CRISPR (clustered regularly interspaced short palindromic repeats) interference targeting 2 genetic variants linked to altered VWF levels and evaluated RAB5C expression by reverse transcription-quantitative polymerase chain reaction. We silenced RAB5C or overexpressed RAB5C wild-type, …

Ultrasonography Of Normal Fetal Kidney Width Measurement For Estimation Of Gestational Age In Sudanese Pregnant Women, Mohamed Omer Mustafa Omer, Mohaned Haleeb Edris, Ali H. Adam, Mohammed A. Abdelmotalab, Hamza Idriss

Iraqi National Journal of Medicine

Background: A precise estimate of gestational age (GA) is essential for providing optimal maternity care and determining the labor date. Aim: This study aimed to determine gestational age between the 14th and 40th weeks for Sudanese pregnant women by measuring the normal fetal kidney width using ultrasound. Methods: The study was designed as a descriptive cross-sectional study in Khartoum and Gezira states, Sudan, from June 2016 to May 2017. Three hundred ninety healthy pregnant women aged between 15 and 45 years were assessed by ultrasound, with overall normal well-being fetuses. Linear regression models for estimating gestational age were developed based …

Evaluation Of D-Dimer Level In Covid-19 Patients, Amal Malik, Noor Kadhim Mohamed-Jawad, Hiba Dawood, Ali Adnan Mohsin

Iraqi National Journal of Medicine

Background: The COVID-19 pandemic is associated with prothrombotic complications, leading to increased morbidity and mortality. D-dimer, a fibrin degradation product, serves as a biomarker for coagulation abnormalities. This study evaluates the role of D-dimer in predicting disease severity and hospitalization in COVID-19 patients. Aim: To assess correlations between D-dimer levels and age, gender, comorbidities, disease severity, and hospitalization needs in COVID-19 patients. Methods: A retrospective analysis of 78 COVID-19-positive patients admitted to an outpatient clinic was conducted. Data on demographics, comorbidities, vaccination status, disease severity, and admission D-dimer levels were analyzed using statistical tests (t-tests, chi-square, Mann-Whitney U). Results: The …

Post-Induction Minimal Residual Disease Study By Flow Cytometry In Pediatric B All And Its Correlation With Initial Clinical And Laboratory Parameters, Huda Ali Alnaseri, Yassmin Ali Alaamiri, Hussam Mahmood Salih

Iraqi National Journal of Medicine

Background: Acute lymphoblastic leukemia (ALL) is a cancer characterized by the rapid and uncontrolled growth of immature lymphoid cells. Among its subtypes, B-lineage ALL in children represents the most frequently diagnosed malignancy in the pediatric population. Despite high survival rates, relapsed or refractory cases remain challenging. Minimal Residual Disease (MRD), detected by flow cytometry, is a strong prognostic marker that guides the intensity of post-induction therapy. Aim: This study aims to evaluate the correlation between post-induction MRD, measured by multicolor flow cytometry, and the initial clinical and laboratory parameters in pediatric patients with B-ALL. Methods: A retrospective cross-sectional study was …

Levels Of 8-Isoprostane And Advanced Oxidation Protein Products In Relation To Sperm Count And Motility, Ahmed Sulaiman, Sherwan Salih

Iraqi National Journal of Medicine

Background: Oxidative stress is implicated in male infertility, with reactive oxygen species contributing to sperm dysfunction. Among oxidative stress markers, 8-isoprostanes (8-isoP) and advanced oxidation protein products (AOPP) serve as indicators of lipid peroxidation and protein oxidation, respectively. However, their relationship with specific sperm abnormalities remains unclear. Aim: This study investigates the oxidative stress markers 8-isoprostane (8-isoP) and advanced oxidation protein products (AOPP) across different semen profiles and explores their correlation with sperm count and motility to evaluate their potential as diagnostic biomarkers in male infertility. Methods: A cross-sectional study was conducted on male partners of infertile couples between May …

Serum Iron Profile In Sudanese Patients Infected By Helicobacter Pylori, Rimaz Gurashi, Abuagla M. Dafalla, Suhair A. Ahmed, Shereen Faisal, Abdelkarim A. Abdrabo

Iraqi National Journal of Medicine

Background: Helicobacter pylori is a Gram-negative, spiral-shaped, motile bacterium that represents one of the most widespread chronic infections in humans, affecting nearly half of the global population. Iron is a vital micronutrient required for almost all living organisms, including H. pylori, which relies on iron to establish colonization. Although several studies have indicated an association between iron deficiency (ID) or iron deficiency anemia (IDA) and H. pylori infection, the precise mechanisms remain incompletely understood. Aim: To assess the serum iron profile among Sudanese patients with H. pylori infection. Methods: Analytical case‑control hospital-based study was conducted at Bahri Hospital, Khartoum state …

Association Of Matricellular Protein, Growth Factor, And Transcription Factor With Molecular Subtypes Of Breast Cancer: A Case Control Study, Marwa Munther Homman, Eman Tariq Ali, Maitham Ali Alrikabi

Iraqi National Journal of Medicine

Background: Breast cancer is a heterogeneous disease comprising molecular subtypes such as Luminal A, Luminal B, Human Epidermal Growth Factor 2 (HER-2)-Enriched, and Triple Negative, each with distinct clinical, molecular, and immunological features. Immunological markers play a critical role in modulating tumor behavior, angiogenesis, and immune evasion. Aim: This study aimed to identify key immunological markers that correlate with the molecular (intrinsic) subtypes of breast cancer and to explore their potential as prognostic factors. Methods: Serum samples from 180 breast cancer patients (56 untreated and 74 treated) were analyzed using Enzyme-Linked Immunosorbent Assay (ELISA) to determine the levels of four …

Dna Methyltransferase Inhibitors In Hematological Malignancies And Solid Tumors, Valentin Wenger, Guillermo Garcia-Manero, Robert Zeiser, Michael Lübbert

Faculty, Staff and Student Publications

Epigenetic modifications such as DNA methylation play a fundamental role in oncogenesis and the progression of neoplasms neoplasias. DNA methyltransferase inhibitors (DNMTi) constitute a family of therapeutic agents that impede the methylation at the 5-position on cytosine nucleotides, thereby modulating the epigenetic regulation of tumor suppressor genes, oncogenes, and other key regulatory genes. The first-generation DNMTi azacitidine and decitabine have demonstrated substantial efficacy in the treatment of medically non-fit, older patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) ineligible for intensive chemotherapy (IC), by virtue of their favorable safety profile. Despite these clinical achievements, however, single-agent DNMTi treatment …

Serendipity In Psychiatric Discoveries: Historical Lessons And Future Imperatives For Clinical Observation, Stanley Lyndon, Vineeth P John

Faculty, Staff and Student Publications

Aims and method: Serendipity has driven many of psychiatry's most important treatments, yet contemporary systems may undermine clinicians' ability to notice and develop unexpected therapeutic effects. This selective narrative review synthesises landmark discovery stories, conceptual accounts of serendipity and contemporary case examples to clarify how chance observations become robust advances.

Results: Across historical and modern examples, serendipitous discoveries consistently reflected the interaction of unexpected events with prepared observers working in supportive institutional and research systems. We identify current barriers created by standardised care, funding and trial structures, and professional fragmentation, and outline a multi-level framework for cultivating serendipity through phenomenological …

Bridging Hypoxia And Vision Loss: The Emerging Role Of Connexins In Local And Systemic Eye Diseases, Xianping Zhang, Yalong Cheng, Jean X Jiang, Yuting Li

Faculty, Staff and Student Publications

Hypoxic eye diseases represent a pivotal yet often underappreciated contributor to the onset and progression of many retinal disorders. When hypoxia persists or exceeds the tissue's compensatory capacity, it triggers pathological retinal neovascularization, blood-retinal barrier disruption, and neuronal apoptosis, ultimately resulting in irreversible visual impairment. Connexins (Cxs) form gap junction channels and hemichannels and regulate retinal cell proliferation, differentiation, and survival, thereby playing a central regulatory role in the pathogenesis of hypoxic ocular diseases. In addition to gap junctions, Cx hemichannels promote transmission of molecules between intra- and extracellular environments, further influencing retinal homeostasis under hypoxic stress. This review synthesizes …