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Pemodelan Regresi Logistik Spasial Pada Kejadian Demam Berdarah Dengue (Dbd) Di Provinsi Jawa Barat Tahun 2023, Valida Ulfa Septiana, Sutanto Priyo Hastono

Jurnal Biostatistik, Kependudukan, dan Informatika Kesehatan

Demam Berdarah Dengue (DBD) merupakan salah satu penyakit menular yang masih menjadi masalah kesehatan masyarakat di Indonesia, termasuk Provinsi Jawa Barat, dengan rata-rata kejadian yang lebih tinggi dari rata-rata nasional. Analisis spasial diperlukan untuk memahami pola penyebaran DBD, mengingat adanya kemungkinan pengaruh antar wilayah. Penelitian ini bertujuan untuk menganalisis pengaruh faktor sosio-demografi, faktor perilaku, serta efek spasial kedekatan geografis antar wilayah terhadap kejadian DBD di Provinsi Jawa Barat tahun 2023. Penelitian ini menggunakan desain potong lintang dengan unit analisis 27 kabupaten/kota di Jawa Barat tahun 2023. Data sekunder diperoleh dari Survei Kesehatan Indonesia 2023 dan Jawa Barat dalam Angka 2024. …

Analysis Of Activated Carbon Fiber (Acf) As Particulate Filtration And Volatile Organic Compound (Voc) Media For The Development Of A Dual-Air Purifying Facepiece Respirator, Brie Mcmahan

ETDs from 2020-2029

Exposure to particulate matter (PM) and volatile organic compounds (VOCs) in occupational or public settings poses adverse health outcomes such as respiratory, cardiovascular, reproductive, neurological, carcinogenic morbidity and mortality. Many occupational settings expose workers to PM and VOC concurrently, yet most commercially available respirators are designed to address either particulates or vapors alone. This includes air-purifying respirators (APRs), powered air-purifying respirators (PAPRs), and filtering facepiece respirators (FFRs) with nuisance-level vapor protection, often limited by bulk, discomfort, high pressure drop, and misuse. We identified a need for alternative dual-exposure media and a respiratory protection device that is lighter, and more comfortable …

Development Of Human Trophoblast Cell Surface Antigen-2 (Trop-2) Specific Radiopharmaceutical Tracers, Arnav Vipulkumar Patel

ETDs from 2020-2029

Trophoblast cell surface antigen 2 (Trop2) is closely related to the development and progression of triple-negative breast cancers (TNBC) and is also being investigated as a pan-cancer biomarker. Trop-2 tumorigenesis is mediated by the calcium ion signaling pathways. By accurately predicting and targeting Trop-2 for immunotherapy, precision therapy is crucial. Trop-2 is overexpressed in tumor tissues compared to normal tissues, which makes it a promising molecular target for cancer-targeted therapy. This thesis reports the development and preclinical evaluation of two novel Trop-2-specific radiopharmaceutical tracers derived from the clinically approved antibody-drug conjugate Sacituzumab Govitecan (SG): a full antibody drug conjugate-based tracer …

Self-Stigma Of Seeking Help, Psychological Safety, And Employee Assistance Program Use In Nurses, Latisha Lenee Marshall

Walden Dissertations and Doctoral Studies

Employee assistance programs (EAPs) remain underutilized across various U.S. work sectors, including nursing. There are several barriers that impede nurses from using EAP services, including self-stigma of seeking help (SSOSH) and psychological safety (PS). The purposes of this study, which used the health promotion model as its theoretical framework, were to determine (a) if there was a difference in the level of SSOSH in nurses who have used EAPs and those who have not used EAPs, (b) if there was a difference in the level of PS in nurses who have used EAPs and nurses who have not used EAPs, …

Surviving Staten Island: Black Women’S Narratives Of Resilience, Motherwork, And Embodied Trauma, Jazmin Rivera

Student Theses

This oral history dissertation centers the experiences of six Black women who had formative experiences navigating hostile school and community-based settings on Staten Island during the 1970s, 1980s, and early 1990s. Guided by a conceptual framework that draws on Black orality, Black feminist thought, and endarkened feminist epistemology, this study demonstrates how Black women were required to cross geographic and social boundaries to survive hostile conditions in Staten Island communities and schools. Findings from this study highlight Black women’s use of community cultural wealth to inform their survival strategies and resilience. At the same time, their experiences indicate the potential …

Expanding Access To Live Donation Opportunities: Implementing Telehealth Solutions To Enhance The Living Donor Navigator Program, Alexis Carter

ETDs from 2020-2029

Living donor kidney transplantation (LDKT) is the most effective treatment for patients with end-stage kidney disease; however, living donation rates have remained suboptimal over the past two decades. As a result, many interventions have been developed and tailored to increase living donation. The Living Donor Navigator (LDN) Program is one effort designed to help transplant candidates identify potential living donors and guide them through the donation process, using a combination of advocacy and systems training. The LDN Program is a lay-navigator-based intervention that equips participants with the knowledge, communication skills, and confidence needed to identify and engage potential living donors. …

Lmod1 Modulates Neointimal Lesion Size After Vessel Injury And Stabilizes Smooth Muscle Cell Gene Regulatory Networks During Atherogenic Stress, Sara Wennersten

ETDs from 2020-2029

Phenotypic switching of smooth muscle cells (SMCs) from a contractile to a synthetic state underlies the pathogenesis of several cardiovascular disease (CVD) pathologies, including restenosis and atherosclerosis. Leiomodin 1 (LMOD1), an SMC-enriched actin-binding protein, has been previously found to regulate SMC phenotypic modulation in vitro. However, its contribution to CVD development and progression, along with its influence on gene regulatory networks (GRNs) that inform underlying responses to stress, remains incompletely defined. Here, I combine a mouse injury model, bulk RNA sequencing, and GRN inference to define how reduced LMOD1 expression perturbs SMC physiology during vascular wall remodeling and lipid stress. …

Full Issue, The Stimulus Team

Stimulus: A Medical Humanities Journal

No abstract provided.

Psychosocial Outcomes In Adults With Cleft Lip And Palate: Exploring The Relationship Between Cleft Severity, Mental Health, And Treatment Satisfaction, Haley Hannabass

ETDs from 2020-2029

Introduction:This study aimed to examine psychosocial outcomes in adults with cleft lip and palate (CL/P), focusing on how cleft severity (unilateral versus bilateral) influences depression, anxiety, social functioning, oral health–related quality of life, and satisfaction with treatment outcomes. Individuals born with clefts often continue to face psychosocial challenges even after surgical repair, and limited research has compared how these outcomes differ by cleft type in adulthood. Materials and Methods:A cross-sectional design was used, including 24 adults aged 18 years and older who had undergone cleft repair and were finished or in active orthodontic treatment at UAB. Participants completed an online …

Sample Preparation Effects On Liquid Scintillation Counting Quench, Jason Rusch

ETDs from 2020-2029

Liquid Scintillation Counting (LSC) is a critical technique in operational health physics, particularly for contamination surveys. However, common, expedient sample preparation choices often introduce unpredictable levels of quench, compromising the accuracy of results. This thesis investigates the impact of common preparation variables including vial material, cap lining, solid sample matrix presence, cocktail volume, and radioactivity. The impact of these sample preparation variables were evaluated using the quench methods on two fundamentally distinct commercial LSC systems, the Perkin Elmer Tri-Carb 4910TR (employing tSIE correction) and the Hidex 600 SL (employing TDCR correction). A total of 320 samples were evaluated on each …

Editorial: Advancing Musculoskeletal Health: Bridging Basic And Clinical Research On Biomechanical Properties Of Joints, Ligaments, Tendons, And Associated Structures, Xin Jin, Snehal S. Shetye, Mengcun Chen

Center for Translational Medicine Faculty Papers

No abstract provided.

Differentiation In The Human Urothelia Is Defined By Distinct Alternative Polyadenylation, Ninh B Le, Surbhi Sona, Briana Santo, Yi Zhang, Rosie Ou, R Allen Schweickart, Veena Kochat, William I Padron, Kunal Rai, Shih-Han Lee, Joo Mi Yi, Oliver Wessely, Byron H Lee, Angela H Ting

Faculty, Staff and Student Publications

Distinct epithelial cell states arise during differentiation, but mechanisms generating transcriptomic diversity among them remain poorly defined. The human ureter urothelium contains basal progenitor, intermediate cells, and terminally differentiated umbrella cells. Prior single-cell RNA sequencing revealed similar global gene expression profiles across these states, raising the question of how distinct identities emerge. Here, we show that alternative cleavage and polyadenylation (APA) introduces a major layer of transcriptomic diversity during urothelial differentiation, largely independent of changes in mRNA levels. Analysis of 13,544 urothelial cells identified hundreds of differentiation-associated APA events. Single-cell imaging revealed spatially specific APA patterns, and reporter assays demonstrated …

Payload Diversification Overcomes Resistance And Guides Sequential Antibody-Drug Conjugate Therapy In Breast Cancer, Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Fujii Takeo, Chalothorn Wannaphut, Jennifer A Maynard, Kyoji Tsuchikama, George W Sledge, Naoto T Ueno, Jangsoon Lee

Faculty, Staff and Student Publications

Purpose: Antibody-drug conjugates (ADCs) have transformed the treatment of breast cancer. FDA-approved ADCs such as trastuzumab deruxtecan (T-DXd), sacituzumab govitecan (SG), and datopotamab deruxtecan (Dato-DXd) have demonstrated clinical benefit; however, drug resistance will almost inevitably emerge in the metastatic setting, and there is no established strategy for selecting subsequent ADCs after disease progression to the first exposure to the ADC. This study aimed to define mechanisms of acquired resistance and evaluate rational sequencing approaches.

Experimental design: We generated breast cancer cell lines resistant to T-DXd (TDXd-R) and SG (SG-R). We assessed antigen expression, internalization, and resistance pathways, including efflux transporter …

Differentiation In The Human Urothelia Is Defined By Distinct Alternative Polyadenylation, Ninh B Le, Surbhi Sona, Briana Santo, Yi Zhang, Rosie Ou, R Allen Schweickart, Veena Kochat, William I Padron, Kunal Rai, Shih-Han Lee, Joo Mi Yi, Oliver Wessely, Byron H Lee, Angela H Ting

Faculty, Staff and Student Publications

Distinct epithelial cell states arise during differentiation, but mechanisms generating transcriptomic diversity among them remain poorly defined. The human ureter urothelium contains basal progenitor, intermediate cells, and terminally differentiated umbrella cells. Prior single-cell RNA sequencing revealed similar global gene expression profiles across these states, raising the question of how distinct identities emerge. Here, we show that alternative cleavage and polyadenylation (APA) introduces a major layer of transcriptomic diversity during urothelial differentiation, largely independent of changes in mRNA levels. Analysis of 13,544 urothelial cells identified hundreds of differentiation-associated APA events. Single-cell imaging revealed spatially specific APA patterns, and reporter assays demonstrated …

Mechanistic Insights Into The Antimicrobial Effect Of Benzodioxane-Benzamides Against Escherichia Coli, Lorenzo Suigo, Alessia Lanzini, Valentina Straniero, William Margolin

Faculty, Staff and Student Publications

Background/Objectives: The bacterial cell division machinery is emerging as an attractive target for antimicrobial compounds. FtsZ, a highly conserved essential division protein, is the target for a number of small molecules such as benzamides. Recent studies show that benzodioxane-benzamides (BDOBs) are among the most potent inhibitors of FtsZ function in Gram-positive bacteria, although their ability to inhibit Gram-negative FtsZ, in particular Escherichia coli FtsZ, has been more controversial.

Methods: Here, we use genetic and cytological methods to demonstrate that FtsZ of efflux pump-disabled E. coli can be efficiently targeted by BDOBs.

Results: We show that engineered mutants …

Assessing Predoctoral Dental Student Interest In Nutrition Education: A Study Across Two Dental Schools, Nadine M. Tassabehji, Xiaoyuan Han, Parvati Iyer Dr., Michelle Brady, Matthew Finkelman, Shafa Nathani, Anna Garrison, Annie Han

Pacific Journal of Health

The mouth is a mirror of overall health. Dentists have a vital role in the diagnosis of oral diseases, prevention, for health promotion. The purposes of our exploratory study were to investigate dental students’ attitudes and perceptions regarding nutrition education and to propose a curriculum checklist for integrating nutrition content into dental curricula.

An optional exploratory survey was distributed at two North American urban dental schools to 351 students, to compare student perspectives regarding implementation of nutrition education. The results were analyzed as a step to improve nutrition education in dental curricula. A validated stepwise checklist was developed to guide …

Increasing Participation Through Online Focus Groups With Adults With Intellectual Disabilities, Emily L. Cary, Chris A. Taylor, Susan Havercamp, Andrea Witwer

Developmental Disabilities Network Journal

The aim of this study was to develop and describe methods on how to conduct virtual focus groups with adults with intellectual disability. Using a community-engaged research approach, adults with intellectual and developmental disabilities helped develop virtual focus group methods. We report on the implementation of these methods through an illustrative application of applying these adaptations to virtual focus groups with 13 adults with intellectual disability in a study assessing their perspectives on mental health therapy.

This work resulted in several adaptations that can enhance inclusivity of focus group research to individuals with intellectual disability. Adaptions broadly included: involving guardians …

An Antioxidant Cocktail Of Tert-Butylhydroquinone And A Manganese Porphyrin Induces Toxic Levels Of Oxidative Stress In Cancer Cells, Sandra Tamarin, Hannah Jung, Joseph Lamorte, Laura Biesterveld, Gabriel Piñero, Grace Turchetta, Molly Myers, Rebecca Oberley-Deegan, Aimee Eggler

Student Papers, Posters & Projects

Despite significant advancement in cancer treatments, therapies with minimal toxicity to healthy cells are still limited. One targetable weakness of cancer cells is their sensitivity to oxidative stress. We find that the combination of two antioxidants—the common food additive tert-butylhydroquinone (tBHQ) and a manganese porphyrin in clinical trials, MnTnBuOE-2-PyP5+ (MnBuOE)—increases oxidative stress and causes apoptotic death in several cancer cell lines, but not in mouse primary fibroblasts. Investigating the mechanism of cell death, MnBuOE is observed to catalyze the oxidation of tBHQ, producing the electrophilic quinone tert-butylquinone (tBQ). A critical role for tBQ and its electrophilic character was revealed with …

A Community Conversation Process To Establish Resident And Service Provider Perspectives On Needs Related To Use And Treatment Of Opioids And Substances, Jennifer Shepheard, Thomas Mundy, Ashlie Watts, Jana Pushkin, Leteisha Gordon, Patrice Shelton, Courtney Blondino, Katherine Werner, Elizabeth Prom-Wormley, Melissa Viray

Health Studies Faculty Publications

Introduction:

Rates of fatal overdose due to opioid and substance use in Richmond, Virginia increased from 44.6 in 2018 to 129.5 per 100,000 city residents in 2023. The underlying contexts surrounding the increase in substance use and overdoses in Richmond, Virginia remains poorly understood.

Methods:

Using community based participatory research principles (CBPR), a series of “community conversations” with neighborhood residents were conducted between May–December 2023. These events included educational information, resource connection, and facilitated qualitative focus group discussion on factors contributing to substance use and overdose, as well as resource needs of people engaged in substance use. Participants also completed …

Fact Sheet: Kratom, Cadence Johnnie, Ashley Yaugher, Aaron Hunt, Cara Murray, Timothy Keady

All Current Publications

There are many common myths and misunderstandings associated with kratom. Kratom (Mitragyna speciosa) is a tropical tree native to Southeast Asia. In recent years, kratom use has grown in the United States. It is sold in various forms, including capsules, powders, and most commonly as liquid extracts or “shots." These kratom products are promoted as health supplements, advertised for benefits such as boosting energy, enhancing mood, or producing opioid-like effects. However, most users may not fully understand what they are consuming or the potential risks it carries. Commercial kratom products vary widely in their chemical composition. Synthetic (human-made) or …

Executive Leadership Education For Every Emergency Medical Services Professional : A Qualitative Study, Joseph D. Danao Ii

Student Dissertations

The Emergency Medical Services (EMS) profession faces numerous challenges in maintaining sufficient capacity to provide frontline medical care amid increasing demand for its services from government officials and the public. The purpose of this qualitative phenomenological study is to explore the experiences of EMS professionals on how the lack of executive leadership education impacts their careers and the viability of their organizations. The problem is that EMS professionals widely lack the executive-level leadership education needed to thrive in their professional careers and ensure the viability of their EMS organizations (Basnawi, 2023; Naidoo et al., 2015). The research questions are developed …

Fermentabilitas Dan Kecernaan Ransum Domba Yang Mengandung Kulit Buah Pisang Muli (In Vitro), Iman Hernaman

Jurnal Ilmu Ternak Universitas Padjadjaran

Kulit buah pisang Muli memiliki potensi sebagai bahan pakan sumber serat pengganti rumput lapang, namun saat ini penggunaannya masih terbatas. Penelitian bertujuan untuk mempelajari penggunaan kulit buah pisang Muli dalam ransum terhadap fermentabilitas dan kecernaan (in vitro). Penelitian menggunakan rancangan acak lengkap dengan 4 perlakuan ransum yang mengandung 10, 20, 30, dan 40% kulit buah pisang Muli. Data yang terkumpul dianalisis dengan menggunakan Uji Duncan. Penelitian menghasilkan data yang memberi petunjuk adanya pengaruh nyata (P3yang terendah yaitu berturut-turut sebesar 161,5 mM, 66,72%, 66,15% dan 1,94 mM. Hasil penelitian menyimpulkan bahwa penggunaan kulit buah pisang Muli dapat sebanyak 40% dapat digunakan …

Performa Domba Lokal Betina Dewasa Pada Berbagai Variasi Lamanya Penggembalaan Di Daerah Irigasi Rentang Kabupaten Majalengka ( Local Ewes Performance At Various Variation Of Length Grazing In Rentang Irigation Area Of Kabupaten Majalengka), Rachmat Somanjaya, Denie Heriyadi, Iman Hernaman

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian mengenai “Performa Domba Lokal Betina Dewasa pada Berbagai Variasi Lamanya Penggembalaan di Daerah Irigasi Rentang Kabupaten Majalengka”, telah dilaksanakan sejak Tanggal 25 Maret sampai 30 Juli 2015. Penelitian ini bertujuan untuk mengukur lamanya waktu penggembalaan optimum terhadap performa domba lokal betina dewasa yang digembalakan di Daerah Irigasi Rentang Kecamatan Jatitujuh Kabupaten Majalengka. Sampel Domba Lokal betina dewasa dalam penelitian ini adalah sebanyak 62 ekor yang tersebar pada variasi penggembalaan 6 jam 22 ekor. Metode penelitian yang digunakan adalah survey dan observasi. Data yang diperoleh dianalisis secara deskriptif, untuk mengetahui perbedaan rata-rata performa domba diuji dengan Analisys of Varian ( …

Lead Levels In Fingernails Of Hemodialysis Patients And Healthy Individuals In Karbala, Iraq: A Biomonitoring Study Using Icp Oes, Baker A. Joda, Rusul Jaafar, Rana Hameed, Hiba Sadeq Alheloo, Sadeq Jaafar Hasan Dwenee

Karbala International Journal of Modern Science

The use of non-invasive media to evaluate the association between trace elements and diseases has spread in recent centuries. Fingernail samples were collected from 103 individuals, including both healthy participants and those undergoing hemodialysis, residing in Karbala, Iraq, and ranging in age from 21 to 75 years. The concentrations of lead were determined using an Inductively Coupled Plasma Optical Emission Spectrometry (ICP OES). Statistical analyses using F-tests and two-tailed t-tests were conducted to examine the effects of health status, gender, smoking status, and their interactions on lead levels in washed fingernails. The concentrations of Pb in washed fingernails of healthy …

Psychological Impacts Of Caring For Individuals With Fluent Versus Nonfluent Aphasia, Allisyn Rae Converse

Student Theses and Dissertations

This investigation sought to explore the mental health differences between caregivers of individuals with fluent aphasia versus caregivers of individuals with nonfluent aphasia with a focus on their levels of depression, anxiety, and stress. This study had a quantitative, non-experimental between group research design, utilizing a Qualtrics survey including the Hospital Anxiety and Depression Scale and the Perceived Stress scale to collect all data. Both caregivers of individuals with nonfluent and fluent aphasia can experience psychological impacts of serving as a caregiver such as elevated stress, anxiety, and depression. However, this study reveals that caregivers of individuals with nonfluent aphasia …

Creative Arts To Enhance Cervical Cancer Awareness Using Art-Based Messages From A Nigerian Crowdsourcing Open Call: Qualitative Thematic Analysis, Abdulhammed O Babatunde, Olufunto A Olusanya, Peter Kalulu, Temitope Ojo, Onyekachukwu Anikamadu, Juliet Iwelunmor, Et Al.

2020-Current year OA Pubs

BACKGROUND: Cervical cancer is a leading cause of cancer deaths among women in Nigeria, yet awareness is low. Historically, art has served as a medium for processing emotions and sharing experiences, which can be effective in promoting health and behavior change.

OBJECTIVE: This study aimed to examine art submissions and social media engagement from a Nigerian crowdsourcing open call to inform co-designed strategies for cervical cancer prevention among women.

METHODS: This study reported following the SRQR (Standard Reporting for Qualitative Research) guideline. From October to November 2023, we launched an open call for art on social media, inviting adult women …

Saga/Atac Complexes Sustain Aberrant Chromatin Regulation And Promote Tumorigenesis In Diffuse Midline Glioma, Rosemary U Richard, Caitlin Bagnetto, Rebecca L Murdaugh, Brittany R Eberl, Alan L Jiao, Adam F Kebede, Barry M Zee, Margaret M Harrington, Mariella G Filbin, Akdes Serin-Harmanci, Yang Shi, Jamie N Anastas

Faculty, Staff and Students Publications

Diffuse midline gliomas (DMG) are aggressive pediatric brain tumors characterized by chromatin and transcriptional dysregulation induced by H3K27M mutations. Strategies for overcoming epigenetic dysfunction to reduce DMG tumorigenesis remain limited. We identified multiple components of the SAGA and ATAC chromatin regulatory complexes as DMG genetic dependencies and found that genetic or pharmacological inhibition of the SAGA/ATAC-associated chromatin reader SGF29 reduces DMG proliferation. Small molecules targeting SAGA/ATAC-associated histone acetylation, ubiquitination, and methylation similarly suppressed DMG growth. Further chromatin profiling and RNAseq analyses reveal that SGF29 controls H3K9ac and H3K4me3 dynamics at both H3K27M-bound and H3K27M-independent target genes linked to proliferation, differentiation, …

Analisis Usia Awal Merokok Remaja Dan Perilaku Merokok Orang Tua Di Indonesia: Studi Gshs 2015, Cathrine Jane David, Qonita Ayunina, Janitra Larasati, Glory Soemoele

Jurnal Biostatistik, Kependudukan, dan Informatika Kesehatan

Kebiasaan merokok masih menjadi permasalahan kesehatan global, termasuk Indonesia. Tingginya prevalensi perokok usia remaja menjadi tantangan kesehatan masyarakat yang salah satu penyebabnya adalah perilaku merokok orang tua. Oleh karena itu, penelitian ini bertujuan untuk menganalisa hubungan antara perilaku merokok orang tua dengan usia awal merokok pada remaja. Penelitian ini menggunakan desain studi potong lintang berdasarkan data Global School-based Student Health Survey (GSHS) 2015. Sampel penelitian ini berjumlah 2027 responden yang dipilih secara acak sistematik. Analisis data dilakukan dengan analisis univariat dan bivariat menggunakan uji Chi-square untuk melihat hubungan antar variabel. Hasil analisis menunjukkan bahwa sebagian besar responden memulai kebiasaan merokok …

Clinical Workflow Of Spine Stereotactic Radiotherapy And Radiosurgery: Insights From A Single-Institution Physics Perspective, Dennis Mackin, Gizem Cifter, Yana Zlateva, Jihong Wang, Yao Ding, Muhammad Shafiq Ul Hassan, Zhiheng Wang, Parmeswaran Diagaradjane, Fada Guan, Travis C Salzillo, Shane Krafft, Jing Li, Martin C Tom, Amol J Ghia, Tina Marie Briere

Faculty, Staff and Student Publications

Spine stereotactic radiotherapy and radiosurgery (SSRS) techniques, encompassing both fractionated stereotactic treatments and single-fraction radiosurgery, are widely used for the management of spinal metastases due to their ability to deliver highly conformal radiation while limiting dose to adjacent critical structures. Clinical outcomes following SSRS, including durable local control and acceptable toxicity, have been reported previously in multiple institutional series. In this manuscript, we describe the clinical workflow used to deliver SSRS at a high-volume academic center, with emphasis on the medical physics processes that support routine clinical practice. Key elements of the workflow include patient selection, treatment region-specific immobilization, CT …

Tumor Cell Clustering Enhances Metastatic Competence By Regulating The H3k36 Histone Demethylase Kdm2a, Carolyn J Kravitz, Kiran Patel, Emily Wingrove, Dejian Zhao, Tang Tang, Sampada Chande, Minghui Zhao, Yuchen Huo, Thomas F Westbrook, Qin Yan, Don X Nguyen

Faculty, Staff and Students Publications

Disseminated tumor cells can form clusters via cell-cell adhesion, which increases their capacity to initiate metastasis. Metastatic clusters are characterized by distinct changes in transcription, suggesting that epigenetic mechanisms underlie their unique phenotypic state. By performing functional epigenomic studies in models of non-small cell lung cancer, we identified the histone H3 lysine 36 (H3K36) demethylase KDM2A as being differentially required for the fitness of metastatic cell clusters. This contextual dependency on KDM2A is predicated by tumor cell-cell aggregation, which specifically induces KDM2A binding to CpG island enriched promoters. At these defined genomic loci, KDM2A maintains H3K36 monomethylation, which preferentially correlates …