Digital Commons Network^™

Noninvasive Enrichment Of Circulating Tumor Biomarkers In A Mouse Model Of Diffuse Midline Glioma Using Focused Ultrasound, Dingyue Zhang, Yimei Yue, Yan Gong, Leqi Yang, Kevin Xu, Jinyun Yuan, Hong Chen

2020-Current year OA Pubs

BACKGROUND: Diagnosing diffuse midline glioma (DMG) through invasive tissue biopsies is challenging due to the tumor's eloquent location at the pons. Liquid biopsies offer a promising noninvasive alternative; however, their limited detection sensitivity and lack of information on the source of biomarkers pose great challenges. This study aimed to evaluate the feasibility and safety of using focused ultrasound (FUS) with microbubbles to enrich circulating DMG tumor biomarkers in blood and cerebrospinal fluid (CSF) using a mouse model.

METHODS: Murine DMG cells, transfected with enhanced green fluorescent protein (EGFP) and firefly luciferase (Fluc) genes, were orthotopically injected into the mouse brain. …

Trastuzumab Deruxtecan In Hormone Receptor-Positive, Her2-Low/-Ultralow Metastatic Breast Cancer (Destiny-Breast06): Outcome Analyses By Time To Progression On Prior First-Line Endocrine Therapy With Cdk4/6 Inhibitor And Baseline Burden Of Disease, G Curigliano, N A Bagegni, Et Al.

2020-Current year OA Pubs

BACKGROUND: In DESTINY-Breast06, trastuzumab deruxtecan (T-DXd) improved progression-free survival (PFS) versus physician's choice of chemotherapy (TPC) for patients with hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-low/-ultralow metastatic breast cancer (mBC), without new safety signals. We report additional analyses exploring outcomes for patients with characteristics that affect prognosis.

PATIENTS AND METHODS: Patients were randomly assigned 1 : 1 to receive T-DXd (5.4 mg/kg) once every 3 weeks or TPC. Subgroups were specified post hoc from the intent-to-treat population (N = 866). Outcomes were PFS, objective response rate (ORR), and duration of response (DOR) by blinded independent central review …

Cabozantinib Plus Nivolumab And Ipilimumab In Previously Untreated, Advanced Renal Cell Carcinoma: Final Results And Biomarker Analyses From The Phase Iii Cosmic-313 Study, R J Motzer, M A Reimers, Et Al.

2020-Current year OA Pubs

BACKGROUND: Primary results from COSMIC-313 demonstrated significantly longer progression-free survival (PFS) with first-line cabozantinib plus nivolumab and ipilimumab versus placebo plus nivolumab and ipilimumab in patients with advanced renal cell carcinoma. Final efficacy and safety results, as well as data from exploratory biomarker analyses, are reported here.

PATIENTS AND METHODS: The design, participants, and primary-endpoint PFS outcomes have been reported previously for this phase III, double-blind, randomized (1 : 1) study of cabozantinib or placebo plus nivolumab and ipilimumab in adults with previously untreated, advanced clear cell renal cell carcinoma. The secondary endpoint was overall survival (OS) in the intention-to-treat …

Biomedgraphica: An All-In-One Platform For Joint Textual Biomedical Prior Knowledge And Numeric Graph Generation, Heming Zhang, Shunning Liang, Tim Xu, Wenyu Li, Di Huang, Yuhan Dong, Philip Miller, Peter Goedegebuure, Marco Sardiello, Jonathan Cooper, William Buchser, Patricia Dickson, Ryan C Fields, Carlos Cruchaga, Yixin Chen, Michael Province, Philip Payne, Fuhai Li, Et Al.

2020-Current year OA Pubs

MOTIVATION: Multiomics data analysis is essential for scientific discovery in precision medicine. However, translating analysis results of omics data analysis into novel scientific hypotheses remains a significant challenge. Human experts must manually review analysis results and generate new hypotheses based on extensive and interconnected biomedical prior knowledge, which is subjective and not scalable. While large language models can accelerate the discovery, their reasoning improves when grounded in structured, auditable, and comprehensive biomedical prior knowledge. However, biomedical knowledge is scattered across heterogeneous databases that use diverse and inconsistent nomenclature systems, making it difficult to integrate resources into a unified format for …

Short Autoinhibitory Sequences Control Phase Separation Of An Essential Bacterial Transcription Termination Factor, Emilia Krypotou, Kiersten M Ruff, Leah K Mckinney, Guy E Townsend Ii, Jue D Wang, Rohit V Pappu, Eduardo A Groisman

2020-Current year OA Pubs

Within cells, across diverse organisms, macromolecular condensation enables spatial and temporal organization of biochemical reactions by organizing proteins and nucleic acids into compositionally distinct membraneless biomolecular condensates. In the gut bacterium Bacteroides thetaiotaomicron, condensate formation by the transcription termination factor Rho (BtRho) increases its termination activity and promotes B. thetaiotaomicron fitness in the mammalian gut. Here, we elucidate the molecular mechanism governing carbon starvation-induced BtRho phase separation. We establish that short, specific amino acid sequences within BtRho's intrinsically disordered region (IDR) control BtRho condensation via complex coacervation. The identified sequences participate in RNA and intra-IDR regulatory interactions that drive condensate …

Neural Plasticity Across Hormonal States In Females: From Modulator To Dynamic Regulator, Takahiro Sasaki, Christine Nwaigwe, Sayaka Inoue

2020-Current year OA Pubs

Ovarian sex steroid hormones play a critical role in regulating fertility. In addition, these hormones influence behavior and mood across the lifespan in females. Converging evidence from rodent studies and human neuroimaging shows that ovarian hormones dynamically regulate neuronal excitability, synaptic efficacy, and structural remodeling across menstrual/estrous cycles, puberty, and reproductive aging. Estrogen and progesterone reorganize network dynamics and gate plasticity eligibility rather than uniformly scaling synaptic strength, with additional contributions from glia, the extracellular matrix, and neurosteroid signaling. Here we synthesize recent cross-scale advances that link structural and functional changes to cellular and circuit mechanisms, and relate these dynamics …

Alcohol Consumption And Molecular Subtypes Of Colorectal Cancer: Pooled Observational And Mendelian Randomization Analyses, Christos V Chalitsios, Yin Cao, Et Al.

2020-Current year OA Pubs

BACKGROUND: Alcohol consumption is associated with colorectal cancer (CRC) risk, yet its association with distinct molecular subtypes remains unclear. Clarifying this could reveal insights into alcohol's carcinogenic mechanisms.

OBJECTIVES: We examined the association between alcohol consumption and the risk of CRC subtypes defined by individual tumor markers (and marker combinations), namely microsatellite instability (MSI) status, CpG island methylator phenotype (CIMP) status, BRAF, and KRAS mutations.

METHODS: Pooled observational (n

RESULTS: Among drinkers, each additional 14 g/d of alcohol was associated with a 10% higher CRC risk [odds ratio (OR) = 1.10; 95% confidence interval (CI): 1.07, 1.13], but this association …

Use Of Wearable Sensors To Measure Improvements In Real World Upper Extremity Activity After Brachial Plexus Reconstruction, Arakua N Welbeck, Catherine E Lang, David M Brogan, Christopher J Dy

2020-Current year OA Pubs

PURPOSE: Current functional assessments after brachial plexus (BP) reconstruction, including surgeon-graded active range of motion and strength scores, and patient-reported outcomes (PROs), have limitations. This study evaluated the feasibility of wearable sensors to quantify recovery and functional outcomes following BP reconstruction. We hypothesized that sensor-derived measures of daily upper-extremity use would correlate with PROs and that real-world upper-extremity use would improve.

METHODS: Traumatic BP injury patients wore bilateral wrist accelerometers for 7-day periods at 3- to 6-month intervals. Data were grouped relative to timing of reconstruction. Duration and magnitude of upper-extremity activity were expressed as ratios of injured to uninjured …

Rapid Antidepressant Potential Of Nitrous Oxide: Current State And Major Questions, Charles F Zorumski, Joseph Cichon, Yukitoshi Izumi, Thomas Zeffiro, Steven Mennerick, Peter Nagele, Charles R Conway

2020-Current year OA Pubs

The success of ketamine, a dissociative anesthetic and non-competitive N-methyl-D-aspartate receptor (NMDAR) antagonist, as a rapidly acting antidepressant has ignited efforts to identify other novel depression treatments. In recent years, several clinical trials indicated that nitrous oxide (N

Fibroblast Growth Factor Receptor Inhibition For Succinate Dehydrogenase-Deficient Gastrointestinal Stromal Tumors: A Phase 2 Trial, Priscilla Merriam, Brian A Van Tine, Et Al.

2020-Current year OA Pubs

While most gastrointestinal stromal tumors are driven by oncogenic mutations in KIT or PDGFRA, 10-15% exhibit functional loss of the succinate dehydrogenase (SDH) complex and genome-wide DNA hypermethylation. Excess methylation in SDH-deficient gastrointestinal stromal tumors disrupts genomic insulators, inducing aberrant expression of oncogenic ligands FGF3, FGF4, and activating an autocrine signaling loop mediated through FGFR1. We conducted a phase 2 trial of pan-fibroblast growth factor receptor inhibitor rogaratinib in patients with sarcoma and report here on the cohort of patients with advanced SDH-deficient GIST. The primary objective was to estimate objective response rate. Secondary objectives were to estimate progression-free survival …

Non-Invasive Profiling Of The Tumour Microenvironment With Spatial Ecotypes, Wubing Zhang, Abul Usmani, Noah Earland, Chloe M Sachs, Peter K Harris, David Y Chen, Et Al.

2020-Current year OA Pubs

Multicellular programs in the tumour microenvironment (TME) drive cancer pathogenesis and response to therapy but remain challenging to identify and profile clinically

Focal White Matter Lesions Drive Grey Matter Inflammation And Synapse Loss, Omar De Faria Jr, Sabine Dietmann, Et Al.

2020-Current year OA Pubs

Focal white matter lesions occur in most neurodegenerative disorders

Retinopathy Caused By A Primary Immune Regulatory Disorder - The Spectrum Of Aire-Associated Retinopathy: Case Series And Literature Review, Mohammad Anas, Andrew C Browning, Siying Lin, Omar A Mahroo, Andrew R Webster, Margaret Reynolds, Tatyana Milman, Ralph C Eagle Jr, Richard Vile, Marc Toso, Gregory S Hageman, Hossein Nazari, Erik J Van Kuijk, Jose S Pulido

2020-Current year OA Pubs

BACKGROUND/OBJECTIVE: Retinal involvement in autoimmune polyendocrine syndrome type 1 (APS1), a rare monogenic autoimmune disorder caused by mutations in the AIRE gene, is increasingly recognised but remains poorly defined. Prior reports suggest a variable phenotype, ranging from mild changes to severe vision loss, often presumed untreatable. We explored the range of retinal phenotypes associated with AIRE gene deficiency in a multicentre case series of patients with APS1.

METHODS: We performed a retrospective case note review of patients with molecularly confirmed APS1 from tertiary ophthalmic centres. Clinical history, multimodal retinal imaging, electrophysiology, genetic data, and treatment regimens were analysed. Histopathology was …

A Scoping Review Of Force Plates In Female Soccer: The Utility, Existing Practice And Identification Of Knowledge Gaps, Jack T. Fahey, Francisco Javier Robles-Palazón, Paul Comfort, Nichoals Ripley

Research outputs 2022 to 2026

Female soccer has grown exponentially with increasing funding, participation and competition. Force plates enable high resolution force-time assessment using isometric and dynamic tasks for acute or longitudinal monitoring, and profiling performance and injury risk. However, more research has been conducted in male soccer players compared to females with the utility of force plates in female soccer yet to be reviewed. The purpose of this scoping review is to review existing research using force plates in female soccer describing the utility of force plates, methods used, reported metrics and to identify gaps in our current understanding. A literature search was conducted …

Age-Related Increase In Plasma P-Tau217 In Amyloid-Beta–Negative Cognitively Unimpaired Individuals Affects Diagnostic Interpretation, Anna E. Mammel, Fernando Gonzalez-Ortiz, Ali Mousavi, Kelsey Hallett, Mary Encarnacion, Don Biehl, Pradip Gill, Sazan Ismael, Christopher Fowler, James D. Doecke, Larry Ward, Hans Frykman

Research outputs 2022 to 2026

Introduction: The extent to which non-pathological aging influences plasma biomarkers remains unclear. Here, we investigate factors influencing plasma p-tau217 levels in cognitively unimpaired (CU), amyloid-beta–negative (Aβ-) individuals. Methods: Plasma p-tau217 was measured in CU Aβ- positron emission tomography–negative (PET-) participants using two immunoassays (ALZpath n = 360 and LUMIPULSE G1200 n = 73). Associations between p-tau217 and age groups (60–69, 70–79, and 80+ years), apolipoprotein E (APOE) genotype, and gender were evaluated. Results: ALZpath plasma p-tau217 showed a non-pathological age-related increase (p < 0.001), and increased percentage within the intermediate zone with age. Lumipulse p-tau217 showed an increase in the percentage of subjects with positive results with age, however, this trend was not significant (p > 0.05). Men had higher levels of p-tau217 only with the ALZpath assay (p = 0.02; Lumipulse: p = …

Exploring The Fidelity Of Resistance Training Interventions In Regional, Rural, And Remote Cancer Care: A Systematic Scoping Review, Christopher W. Rowe, Nicolas H. Hart, Julia Carlino, James Murray

Research outputs 2022 to 2026

Resistance-based exercise interventions are an integral part of supportive cancer care, though their high quality and high-fidelity implementation with people with cancer living in non-metropolitan locations is challenging. This systematic scoping review aimed to identify how exercise prescription principles and measures of fidelity are reported in resistance-based exercise interventions delivered to people with cancer living in regional, rural and remote areas. A systematic scoping review was conducted in accordance with the PRISMA-ScR guidelines. Five electronic databases were searched (MEDLINE/PubMed, Embase, CINAHL, SCOPUS and SportDiscus) for peer-reviewed studies published in English, that delivered a resistance-based exercise intervention to adult cancer survivors …

Influence Of Intended Slow And Fast Eccentric Back Squat Velocity On Subsequent Countermovement Jump Performance, Artemis Zarkadoula, Themistoklis Tsatalas, Anthony D. Kay, Anthony J. Blazevich, Christos Kokkotis, Spyridon Plakias, Brett Anthony Baxter, Alex J. Van Enis, Giannis Giakas, Minas A. Mina

Research outputs 2022 to 2026

Background: The back squat is a key strength and conditioning exercise used to develop lower-limb strength and power, yet little is known about how movement velocity influences its acute performance-enhancing effects, such as improvements in countermovement jump height and power. The present study examined the acute effects of slow (vslow) versus fast (vfast) eccentric-phase velocity during back squats performed withmaximal concentric velocity on subsequent countermovement jump (CMJ) performance, using a randomized, crossover design. Methods: Fourteen male subjects (age = 22.9 ± 1.9 years; height = 1.8 ± 0.1 m; mass = 76.4 ± 8.3 kg) visited the laboratory on two …

Peripheral Neuromuscular Fatigue Responses Of The Knee Extensors To Distinct Concurrent Training Protocols: A Preliminary Study, Tomás T. Freitas, Elena Marín-Cascales, Cristian Marín-Pagán, Linda H. Chung, Antonio Martínez-Serrano, Nicola A. Maffiuletti, Anthony J. Blazevich, Pedro E. Alcaraz

Research outputs 2022 to 2026

Background: This study aimed to investigate the extent and time course of peripheral neuromuscular fatigue of the knee extensors following different concurrent training protocols in recreationally active men. Methods: In a randomized, counterbalanced, crossover design, ten participants completed one exercise session of three concurrent exercise protocols in consecutive weeks and in similar resting conditions: traditional concurrent training (TCT), sprint interval training (SIT), and high-intensity resistance circuit training (HRC). Maximal voluntary isometric contraction (MVIC) and electrically evoked tetanic force of the knee extensors were assessed before, immediately after, and at 24 and 48 h following each exercise session. Linear mixed models …

The Crossroads Between Osteosarcopenia And Intrinsic Capacity—A Narrative Review, Nailton J. Neto, Guy Hajj-Boutros, Wayne Lok Ok Choo, Miguel G. Borda, Konstantinos Prokopidis, Hassan Rammal, Daniel Rivas, Hussein Samhat, Ivan Baltasar-Fernandez, Mahdi Imani, Cristiano Dos Santos Gomes, Oscar Rosas Carrasco, Marc Sim, Julie A. Pasco, Julia Chabot, Ana M. Ayala-Copete, Francisco J. García-García, Claire Godard-Sebillotte, Raman Agnihotram, Lana J. Williams, Anna Andrianova, Robinson Ramirez-Vélez, Emma Connolly, Harmehr Sekhon, Hidenori Arai, Liang-Kung Chen, Andréa Faust, Howard Bergman, Alexandra Papaioannou, Pierrette Gaudreau, Tiago Alexandre, Leocadio Rodriguez-Mañas, Ricardo Oliveira Guerra, Gustavo Duque

Research outputs 2022 to 2026

Intrinsic capacity (IC) is defined as the composite of physical and mental abilities an individual possesses, encompassing 5 domains: cognition, psychological health, sensory function, vitality, and locomotion. This construct is central to the World Health Organization’s framework for assessing functional ability in older adults. Growing evidence highlights the critical role of the musculoskeletal system in maintaining these domains, while conditions such as sarcopenia, osteoporosis, and their coexistence as osteosarcopenia (OS) are increasingly associated with IC decline. This narrative review compiles current evidence on the modulatory role of muscles and bones in IC and the impacts of sarcopenia, osteoporosis, and OS. …

The Effect Of Boosting Dietary Lactobacillus And Phytochemical Rich Foods On Biomarkers Of Longevity—A Phase Ii Randomised Placebo Controlled Trial, Robert J. Thomas, Madeleine Williams, Jeffrey W.F. Aldous, Stacey A. Kenfield, Robert U. Newton

Research outputs 2022 to 2026

As men age, systemic inflammation increases, whereas grip strength (GS) and testosterone levels tend to fall. This rate of decline is known to be influenced by gut microbial health, lifestyle and diet but the role for interventions to slow this decline are less well established. This double-blind randomised controlled trial evaluated the impact, and explored the synergistic potential, of boosting phytochemical rich foods and Lactobacillus probiotics on these physical and biological markers. Two hundred and eight men with untreated early prostate cancer managed with observation only (average age 74 years) received a phytochemical rich supplement (PRS) containing concentrated broccoli, green …

Standardizing Interprofessional Serious Illness Conversations In Inpatient Specialty Palliative Care, Leah Mccann Klug, Catherine Patterson

All Poster Presentations

No abstract provided.

Beyond Ce Credit: A Retrospective Analysis Of Cme And Cpe Impact In A Health System, Diem Pham, Michelle Eng, Brandon Wu

All Poster Presentations

No abstract provided.

Enhancing Lactation Support In The Icu Via Interdisciplinary Collaboration, Lexxi Ellis, Aretha Miller

All Poster Presentations

No abstract provided.

Nurses' Perspectives On Caring For Critically Ill Children During A Measles Outbreak: A Case Report, Nicole Roehrig

All Podium Presentations

No abstract provided.

Quality Improvement: Referral Algorithm For Team-Based Care Of Blood Pressure Control, Hai-Yen Tang, Elise Gibson

All Podium Presentations

No abstract provided.

Beyond Ce Credit: A Retrospective Analysis Of Cme And Cpe Impact In A Health System, Diem Pham, Michelle Eng, Brandon Wu

All Podium Presentations

No abstract provided.

Evaluation Of The Impact Of Pharmacist-Led Toc On Patient Care Outcomes, Taabris Jaamal, Hana Breeland, Thanh Nguyen, Barbara Branneman, Jack Huber, Hung Phan

All Poster Presentations

No abstract provided.

Impacts Of The Early Collaborative Intervention On Mother-Preterm Infant Interaction At One Month Of Age: Secondary Analysis Of A Randomized Controlled Trial, Charlotte Sahlén Helmer, Ulrika Birberg Thornberg, Thomas Abrahamsson, Evalotte Mörelius

Research outputs 2022 to 2026

Background A sensitive, well-functioning maternal interaction is vital for healthy infant development. For moderate to late preterm infants, this is even more important as this group of infants are at increased risk of facing neurodevelopmental disorders. The Early Collaborative Intervention supports parent-preterm infant interaction and includes three sessions, two in the hospital and one after discharge. Objective To investigate the impact of the Early Collaborative Intervention, compared with standard care, on mother-preterm infant interaction at one month corrected age. Design A longitudinal randomized controlled trial, reporting secondary outcomes from the first follow-up. Setting The intervention was conducted at a pediatric …

Precision Nutrition In Breast Cancer: Towards Patient- And Tumour-Informed Dietary Strategies, Cristina Crespo-García, R. Taaffe, Carolyn Peddle-Mcintyre, Emily Jeffery, John P. Campbell, Robert Thomas, Daniel A. Galvao, Robert U. Newton

Research outputs 2022 to 2026

Dietary strategies are increasingly recognized as important modulators of breast cancer outcomes, acting through effects on metabolic regulation, weight management, hormone signalling, immune function, and the gut microbiome. However, breast cancer heterogeneity and inter-individual variability mean that generic dietary guidelines may not fully capture patient needs. Precision nutrition offers the opportunity to align dietary interventions with tumour subtype, treatment context, and host biology, potentially enhancing therapeutic response and survivorship in patients diagnosed with breast cancer. In this narrative review, we summarize evidence on dietary patterns and prognosis, and explore how targeted interventions, including fasting regimens, ketogenic diets, and caloric restriction, …

Shape-Based Profiling Of Cortical Bone Mineral Density In Growing Children: A Proof-Of-Concept Pqct Study, Anna Maria Markarian, R. Taaffe, Daniel Galvão, Jodie Cochrane Wilkie, Carolyn J. Peddle-Mcintyre, Daniel J. Schiferl, Robert U. Newton

Research outputs 2022 to 2026

Background: Understanding how bones adapt during growth is essential for promoting lifelong skeletal health. Peripheral quantitative computed tomography (pQCT) is a widely used imaging technique for assessing volumetric bone mineral density (vBMD) in pediatric populations; however, conventional methods may obscure subtle cortical adaptations. The primary aim of this study was to evaluate the feasibility of shape-based cortical vBMD profiling, with an exploratory objective of assessing its sensitivity to inter-individual variation in lean mass index (LMI). Methods: Eighteen children (6–12 years) underwent pQCT scanning at the 38% and 66% tibial sites (distal and proximal diaphysis). Cortical vBMD was quantified across three …