Digital Commons Network^™

Hp1bp3 Loss Links Chromatin Reorganization To Metabolic Vulnerability In Glioma, Brittney Lozzi, Taylor A Gatesman, Pushan Dasgupta, Debosmita Sardar, Yeunjung Ko, Chenyu Mao, Hsiao-Chi Chen, Rachel N Curry, Dongjoo Choi, Carrie A Mohila, Melissa L Bondy, Ganesh Rao, Marco Gallo, Sameer Agnihotri, Benjamin Deneen

Duncan NRI Faculty and Staff Publications

High-grade gliomas (HGGs) are aggressive brain tumors with poor prognosis, driven in part by metabolic and epigenetic adaptations. Methionine metabolism supports HGG growth by supplying S-adenosylmethionine for methylation reactions, yet how nutrient availability influences chromatin organization in HGG remains incompletely understood. Using an immunocompetent mouse model of HGG, we found that dietary methionine restriction reduced tumor proliferation, extended survival, and induced partial nuclear inversion. We identified Hp1bp3 as a key regulator of tumor growth that functions by interacting with nuclear tethering proteins to mediate chromatin reorganization. Loss of Hp1bp3 results in the upregulation of histone demethylases leading to selective depletion …

Broadly Reactive Human Monoclonal Antibodies Targeting Influenza A Nucleoprotein Lack Protective Activity In Vivo, Walter N Harrington, David C Brice, Aaron J Schmitz, Jackson S Turner, Rafael Brent, Philip A Mudd, Ali H Ellebedy, Richard J Webby

2020-Current year OA Pubs

The antibody response to influenza virus infection targets numerous viral proteins, with those specific for the hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins being the most extensively studied. Antibodies to HA and NA function as independent immune correlates of protection and form the basis of most vaccine approaches. In contrast, the contribution of antibodies to other viral proteins such as the nucleoprotein (NP) remains unclear, and existing studies have reached conflicting conclusions. In this work, we examined three human monoclonal NP antibodies isolated from plasmablasts of two individuals naturally infected with influenza A virus (IAV) during the 2018-2019 influenza season. …

Forming Physicians For Others: The Identity Of The Jesuit Catholic Medical School, John Hardt, David G. De Marco Sj

Jesuit Higher Education: A Journal

Forming Physicians for Others: The Identity of the Jesuit Catholic Medical School

The Jesuit medical school accepts a widely-acknowledged telos: the preparation of the medical student to offer excellent patient care. It pursues this telos, however, through the distinctiveness of Ignatian formation, which finds expression in each domain of undergraduate medical education. This formative commitment, oriented toward and witnessing to an appreciation of the unconditional worth of the human being, may empower a subset of medical students—who are engaged by a life of faith and open to the transcendent invitation of their lives—to experience an even more fundamental transformation—one that …

Pa Dent J Jan 2017

Pennsylvania Dental Journal

The Pennsylvania Dental Journal is produced by the Pennsylvania Dental Association six times a year, in January, March, May, July, September and November. Issues include regular columns with regional news impacting the Pennsylvania membership including editorial and government relations news, association activities, member updates, continuing education opportunities, and classifieds.

Pa Dent J Nov 2021

Pennsylvania Dental Journal

The Pennsylvania Dental Journal is produced by the Pennsylvania Dental Association six times a year, in January, March, May, July, September and November. Issues include regular columns with regional news impacting the Pennsylvania membership including editorial and government relations news, association activities, member updates, continuing education opportunities, and classifieds.

Pa Dent J May 2019

Pennsylvania Dental Journal

The Pennsylvania Dental Journal is produced by the Pennsylvania Dental Association six times a year, in January, March, May, July, September and November. Issues include regular columns with regional news impacting the Pennsylvania membership including editorial and government relations news, association activities, member updates, continuing education opportunities, and classifieds.

Pa Dent J March 2024

Pennsylvania Dental Journal

The Pennsylvania Dental Journal is produced by the Pennsylvania Dental Association six times a year, in January, March, May, July, September and November. Issues include regular columns with regional news impacting the Pennsylvania membership including editorial and government relations news, association activities, member updates, continuing education opportunities, and classifieds.

Pa Dent J Jan 2023

Pennsylvania Dental Journal

The Pennsylvania Dental Journal is produced by the Pennsylvania Dental Association six times a year, in January, March, May, July, September and November. Issues include regular columns with regional news impacting the Pennsylvania membership including editorial and government relations news, association activities, member updates, continuing education opportunities, and classifieds.

Community - Volume 11

be Still

No abstract provided.

In That Space, We Were Communis, Eliana Namen

be Still

In reflecting on my understanding of community, there were many moments I could have chosen to write about. Yet the memory of my grandmother’s death remains the one most deeply etched in me. When I began considering what community truly meant in my own life, I found myself returning to that room. In her illness and eventual passing, she drew together an extraordinary constellation of people: family, friends, neighbors, whose lives she had quietly shaped. Her life created ripples far beyond what any one person could see. In witnessing that gathering, I came to understand community not merely as shared …

Using Social Return On Investment To Evaluate Services That Support The Health Of People Experiencing Homelessness In Australia: A Scoping Review, Cate Mckenna, Christopher Rouen, Mary O'Loughlin

Research outputs 2022 to 2026

The increasing demand for homelessness services in Australia highlights the need for robust evaluation methodologies to assess their social impact. Traditional economic evaluation methods often struggle to capture the intangible social and health outcomes associated with homelessness. Social return on investment (SROI), an outcomes-based framework driven by stakeholders, offers a flexible and comprehensive approach to measuring social, economic and environmental impacts. This scoping review examines the application of SROI in evaluating services that support the health of people experiencing or at risk of homelessness in Australia. A total of 25 reports from grey literature (2010-2023) were reviewed. The findings reveal …

Exploring Moral Distress In Pediatric Residents: Impact Of Structured Debriefing And Insights From Qualitative Interviews, Aashish Shah, Sarah Fleishaker, Danielle Key, Linda Tewksbury, Ami Shah, Kelley Groves

Journal of Wellness

No abstract provided.

Autophagic Flux Blockade Under Hypocapnia Reveals Co2-Sensitive Regulation Of Autophagy-Lysosome Homeostasis, Naghmana Ashraf, Zhen Sun, Jeanine L Van Nostrand

Faculty, Staff and Students Publications

Hypocapnia, a reduction in partial pressure of carbon dioxide (CO2), commonly occurs in clinical contexts such as mechanical ventilation, panic disorder, and brain injury, yet its impact on cellular homeostasis remains poorly understood. Given the central role of autophagy in stress adaptation, we investigated how low CO2 influences autophagic flux and lysosomal function. We found that hypocapnia induces autophagosome accumulation while impairing cargo degradation, indicating a blockade in autophagic flux. This response was accompanied by increased lysosome biogenesis but, paradoxically, reduced autophagosome-lysosome fusion and lysosomal proteolytic activity. Mechanistically, hypocapnia promoted TFE3 dephosphorylation and nuclear translocation, driving transcriptional activation of lysosomal …

Macropinocytosis Inhibition Attenuates Profibrotic Responses In Lung Fibroblasts And Pulmonary Fibrosis Models, Ivan O Rosas, Aaron K Mcdowell-Sanchez, Santiago Sanchez, Juan D Cala-Garcia, Alan R Waich Cohen, Elisa Ruiz-Echartea, Scott A Ochsner, Daniel C Kraushaar, Lindsay J Celada, Dandan Sun, Francesca Polverino, Cristian Coarfa, Neil J Mckenna, Konstantin Tsoyi

Faculty, Staff and Students Publications

Idiopathic pulmonary fibrosis (IPF) is a devastating chronic lung disorder with limited treatment options. Macropinocytosis is one of the key cellular processes involved in nutrient consumption from the extracellular environment under stress conditions. Here, we studied the role of macropinocytosis in experimental pulmonary fibrosis models. We found that macropinocytosis is increased in human lung fibroblasts (HLFs) derived from patients with IPF. The inhibition of macropinocytosis with 5-(n-ethyl-n-isopropyl)-amiloride (EIPA) inhibited profibrotic responses in IPF-derived and TGF-β1-stimulated HLFs and reduced pulmonary fibrosis in bleomycin-injured (Bleo-injured) mice. EIPA exerted its antifibrotic effects by regulating amino acid uptake, mammalian target of rapamycin complex 1 …

Preventing Long-Term Harm: Pediatric Pain Management In Primary Care, Mike Smith, Amanda D. Deacy

Pediatrics in Practice: A CME Podcast

This episode explains practical steps primary care clinicians can take to recognize and reduce procedural pain and emotional distress in children, and why early management matters for long-term health and trust in healthcare. Guest Amanda Deacy, PhD, Clinical Director of the Abdominal Pain Program and pediatric psychologist at Children’s Mercy, reviews evidence-based strategies and how they prevent chronic pain and vaccine avoidance. Learn about pediatric pain management, needle procedures, topical anesthetic options, comfort positioning, distraction techniques, and vaccine pain reduction.

Click here to stream the podcast episode.

Antimicrobial Susceptibility And Targeted Molecular Detection Of Methicillin Resistance Determinants In Staphylococcus Spp. Isolated From Broiler Bco Lesions, Woro Wulandari Kalanjati, Chrystalee Ailani Alvarez, Anh Dang Trieu Do, Adnan Ali Khalaf Alrubaye

School of Integrative Biological & Chemical Sciences Faculty Publications

Background/Objectives: Antimicrobial resistance (AMR) in Staphylococcus spp. associated with poultry production is an emerging concern with implications for animal and public health. This study aimed to characterize antimicrobial susceptibility patterns and detect targeted methicillin resistance determinants in Staphylococcus isolates recovered from broiler chickens affected by bacterial chondronecrosis with osteomyelitis (BCO). Methods: A total of 200 bacterial isolates were evaluated, of which 167 were confirmed as Staphylococcus spp. Species identification was performed using presumptive phenotypic characterization followed by 16S rRNA gene sequencing. Antimicrobial susceptibility was assessed using disk diffusion, while presumptive methicillin-resistant phenotypes were evaluated using oxacillin screening and CHROMagar MRSA. …

The Neuroprotective Effect Of Cdc42 Inhibition In The G93a Mutant Hsod1 Mouse Model Of Als, Andrea Koly

Undergraduate Theses, Capstones, and Recitals

Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease characterized by the selective loss of upper and lower motor neurons, leading to muscle weakness, neuromuscular junction (NMJ) degeneration, paralysis, and eventual death due to respiratory failure. Despite extensive research, effective disease-modifying therapies remain limited, underscoring the need to identify novel molecular targets involved in ALS pathogenesis. Dysregulation of Rho family GTPases has been implicated in neurodegeneration, with Rac and Rho exerting opposing effects on neuronal survival; however, the role of the closely related GTPase Cdc42 remains poorly understood in ALS. In this study, the therapeutic potential of inhibiting …

Differential Roles Of Moesin And Vasp In Β-Catenin-Associated Networks During Zebrafish Heart Regeneration, Allen Rutman

Undergraduate Theses, Capstones, and Recitals

With cardiovascular disease remaining as the leading cause of mortality worldwide, it is largely due to the limited regenerative capacity of the human heart. However, zebrafish (Danio rerio) can fully regenerate cardiac tissue following injury, providing a valuable model for studying the mechanisms of heart repair. To investigate these mechanisms, we used proteomics methods in the past. Proximity labeling is a technique that identifies proteins based on their physical proximity to a protein of interest. It can be applied in organisms and in living cells (Roux et al., 2012; Cho et al., 2020). We used proximity labeling to characterize protein …

2nd National Research Day Lebanese Medical Schools

MEAMS Middle East Annals of Medical Sciences - Saint Joseph University of Beirut

No abstract provided.

Rural–Urban Disparities In Monkeypox-Related Behaviors And Attitudes Among Men Who Have Sex With Men, Patcharaporn Krainara, Benjamas Klahan, Rungrot Jaiyong, Jitti Hanprasertpong

Journal of Health Research

Background: Monkeypox (Mpox) is a global health threat, particularly among men who have sex with men (MSM). Despite the predominance of urban cases in Thailand, rural–urban disparities in Mpox-related attitudes and behaviors remain underinvestigated. This study aimed to compare Mpox-related attitudes and preventive behaviors among urban (Chonburi) and rural (Nakhon Nayok) MSM and identify their influencing factors.

Method: This cross-sectional study was conducted between March and April 2025. A total of  842 MSM (421 urban; 421 rural) were recruited via convenience sampling. Data were collected using paper-based, self-administered Health Belief Model (HBM) questionnaires and analyzed using descriptive statistics and multivariable …

Staff Education On Triage For Resource Nurses In The Emergency Department, Michelle Donahue Smeltzer

Walden Dissertations and Doctoral Studies

No abstract provided.

Flame Retardant Metabolites In Urine Samples Associated With U.S. Consumer Dietary Behaviors, Heather Marie Holliday

Walden Dissertations and Doctoral Studies

No abstract provided.

Comparison Of Bacteriological Culture And A Multiplex Real-Time Pcr Kit For The Detection Of Mastitis Pathogens In Small Ruminants, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Bariş Güner, Öznur Yilmaz Koç, Ali̇ Ünver, Merve Yildiz, İrem Çakmak, Abdulkadi̇r Keski̇n

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate the applicability of a multiplex real-time PCR (rPCR) kit, originally developed for bovine mastitis, for detecting mastitis pathogens in small ruminants and reducing bacterial identification time compared with conventional bacteriological culture (BC). Milk samples were obtained from 175 sheep and 124 goats and included cases of clinical mastitis (n = 57) and subclinical mastitis (n = 242). With BC, bacterial growth was detected in 125 of 299 milk samples (41.8%), yielding a total of 169 bacterial isolates. The pathogens most frequently isolated by BC were coagulase-negative staphylococci (58.0%), followed by Streptococcus spp. (9.4%), Staphylococcus aureus …

Comparative Efficacy Of Controlled Internal Drug Release And Medroxyprogesterone Acetate Sponges On Ovulatory Follicle Growth In The Ovsynch Protocol During The Breeding Season In Cyclic Nili Ravi Buffaloes, Muhammad Binyameen, Ali Husnain, Saba Anwar, Muhammad Waseem, Muhammad Saleem

Turkish Journal of Veterinary & Animal Sciences

The objective of the experiment was to compare the dominant follicle size, growth rate of the dominant follicle, estrus response, plasma progesterone concentration, and pregnancy in Nili Ravi buffaloes synchronized by controlled internal drug release (CIDR; 1.38 g) and medroxyprogesterone acetate sponge (MPAS; 250 mg) in the ovulation synchronization (OvSynch) protocol. The experiment had a randomized complete block design. Buffaloes (n = 42) were blocked by parity, body weight, body condition score, and ovarian status, and, within the block, they were randomized to receive either CIDR or MPAS and that day was considered day 0 of the experiment. On days …

Determination Of Hematobiochemical Parameters And Egg Quality Traits Of Turkish Sultan Chickens, Mehmet Naci̇ Oktay, Ruhi̇ Kabakçi, Ali̇ Karapinar, Gökhan Şen, Taha Altuğ, Yasi̇n Şenel

Turkish Journal of Veterinary & Animal Sciences

The present study aimed to investigate the hematological and biochemical blood parameters, along with selected egg quality traits, of Turkish Sultan chickens. A total of 90 blood samples collected from 60-week-old hens and roosters and 129 eggs collected from 50 hens were analyzed. The findings showed that hematological parameters such as red blood cells (RBC), hemoglobin (HGB), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and heterophil-to-lymphocyte (H/L) ratio, as well as biochemical markers such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and uric acid levels, were significantly higher in roosters. The average egg weight, shape …

The In Vitro Feeding Of Hyalomma Marginatum (Koch, 1844), Nesli̇han Ölmez, Bariş Sari, Zati̇ Vatansever

Turkish Journal of Veterinary & Animal Sciences

Ticks are obligate hematophagous ectoparasites that play a major role in the transmission of pathogens affecting both animals and humans. Investigations into tick biology, pathogen transmission, and the development of effective control strategies have traditionally depended on the extensive use of laboratory animals. Consequently, the development of reliable in vitro feeding systems is a valuable alternative approach with the potential to reduce animal use in tick research. In the present study, a previously described silicone membrane artificial feeding system was modified and applied for the in vitro feeding of Hyalomma marginatum (Koch, 1844). Feeding units were prepared using bovine blood …

Immunohistochemical Expression Of Lgr5 In The Bovine Ovary, Oviduct, And Uterus, Aytül Kürüm, Yasi̇n Özkabadayi, Hakan Kocamiş, Mustafa Türk, Si̇yami̇ Karahan

Turkish Journal of Veterinary & Animal Sciences

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) has recently been considered as a stem cell marker seen especially in mitotically active tissues. The aim of the present study was to investigate the immunohistochemical expression of LGR5 in the bovine reproductive tract, which is regularly renewed in response to hormonal changes throughout the estrous cycle. The oviduct (ampulla, isthmus, and fimbria), uterus, and ovary tissue samples were collected from 12 cows at a local slaughterhouse. The samples were further identified as follicular (n = 6) or luteal (n = 6), subjected to routine histological procedures, and processed for immunohistochemical expression of …

Origins Of Mitochondrial Diversity In Turkish Sheep Breeds: Anatolia As A Contact Zone Or Refugial Center?, Nezi̇h Ata, İbrahi̇m Cemal

Turkish Journal of Veterinary & Animal Sciences

Advances in molecular genetics, especially the integration of mitochondrial DNA (mtDNA) analyses, have significantly transformed our understanding of the origins, geographical centers, and dispersal dynamics of domestication, a field traditionally based on archaeological and morphological evidence. In this study, the mtDNA control-region sequences of 296 individuals from 15 native Turkish sheep breeds were analyzed to clarify their maternal genetic diversity and evolutionary origins. Sequencing of a 531-bp D-loop fragment revealed 53 polymorphic sites and 78 distinct haplotypes, indicating substantial haplotypic diversity (Hd = 0.918) and nucleotide diversity (π = 0.01391) within these populations. All five major ovine mitochondrial haplogroups (A, …

Single-Nucleus Profiling Reveals A Core Disease Signature And Cell Type-Specific Vulnerabilities In Early Rett Syndrome, Yan Li, Ashley G Anderson, Guantong Qi, Sih-Rong Wu, Jean-Pierre Revelli, Hu Chen, Zhandong Liu, Huda Y Zoghbi

Faculty, Staff and Students Publications

Rett syndrome (RTT) is an X-linked neurological disorder caused by MECP2 mutations, creating distinct cellular environments in females (mosaic) versus males (nonmosaic). Despite female patients representing most cases, how mosaicism contributes molecularly to RTT pathogenesis, particularly in presymptomatic stages, remains poorly understood. To address this question, we profiled hippocampal transcriptomes of young female and male RTT mice using bulk and single-nucleus RNA sequencing. We identified a core disease signature of consistently dysregulated genes only in MeCP2− cells across RTT models. Moreover, we uncovered non–cell autonomous effects exclusively in female MeCP2+ excitatory neurons, suggesting that these circuits are more vulnerable early …

Artificial Intelligence In Biomedical Scientific Publishing, Tomasz J Guzik, Victor Aboyans, Stefan Agewall, Steven Bailey, Adrian Baranchuk, Magnus Bäck, Michael Böhm, Robert O Bonow, George W Booz, Giuseppe Boriani, Biykem Bozkurt, Nico Bruining, Davide Capodanno, Çetin Erol, Péter Ferdinandy, Alessia Gimelli, Gerd Heusch, Thomas Kahan, Chim Choy Lang, Gerald Maurer, Robert J Mentz, Marco Metra, Philip Moons, Daniel Piskorz, Massimo Piepoli, Piotr Ponikowski, Alexandra Popma, Bianca Rocca, Juan Sanchis Fores, Philipp Sommer, Rhian M Touyz, Zoltan Ungvari, Pascal Vranckx, Joseph A Hill, Thomas F Lüscher, Filippo Crea

Faculty, Staff and Students Publications

Artificial intelligence is now embedded across the scientific research and publishing ecosystem, influencing discovery, analysis, knowledge translation, authorship, peer review, and editorial workflows. In cardiovascular and biomedical sciences, these developments offer substantial opportunities to accelerate knowledge generation, integrate complex datasets, and improve efficiency and consistency. At the same time, they introduce new risks related to bias, transparency, data integrity, and authorship responsibility, potentially endangering trust in the scientific record. This commentary examines the evolving role of AI in biomedical publishing, with particular attention to generative models and machine learning tools. We review both benefits and limitations, highlight risks such as …