Digital Commons Network^™

Respon Broiler Terhadap Pemberian Ransum Mengandung Dedak Padi Fermentasi Oleh Kapang Aspergillus Ficuum (Response Of Broiler Fed On Diet Containing Fermented Rice Bran By Aspergillus Ficuum), Siti Wahyuni H.S., Dwi Cipto Budinuryanto, H. Supratman

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian bertujuan untuk mengetahui respon broiler terhadap pemberian ransum mengandung dedak padi hasil fermentasi oleh Aspergillus ficuum yang diukur melalui konsumsi ransum, pertambahan bobot badan, konversi ransum, persentase karkas dan imbangan efisiensi protein.  Penelitian menggunakan 300 ekor ayam broiler final stock Arbor Acres yang dibagi ke dalam 30 plot.  Ransum disusun iso-protein dan iso-energi dengan kandungan protein kasar 22% dan energi metabolis 3100 kkal/kg.  Penelitian dilakukan secara eksperimen menggunakan Rancangan Acak Lengkap , terdiri atas 6 ransum sebagai perlakuan setiap perlakuan diulang 5 kali.  Ransum perlakuan terdiri atas R0 (0% dedak padi fermentasi), R1 (5% dedak padi fermentasi), R2 (10% …

Studi Pembuatan Kompleks Mineral-Minyak Dan Efek Penggunaannya Dalam Ransum Terhadap Fermentabilitas Dan Kecernaan (In Vitro) (Study Of Oil-Mineral Complex And It Effects On In Vitro Fermentability And Digestibility), U. Hidayat Tanuwiria, Dwi Cipto Budinuryanto, S. Darodjah, Wendry Setiyadi Putranto

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum yang mengandung berbagai kompleks minyak-mineral in vitro.  Penelitian dilakuan secara eksperimen dengan Rancangan Acak Lengkap (RAL) empat perlakuan dan lima ulangan. Percobaan pertama terdiri atas R1 = Ransum lengkap + 5% Minyak Jagung, R2 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Jagung, R3 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Kacang Tanah, R4 = Ransum lengkap + 5,95% Kompleks Ca-Minyak Ikan. Perlakuan kedua : R1 = Ransum lengkap + 5% Minyak Kacang Tanah. R2 = Ransum lengkap + 5% Kompleks I-Minyak Jagung, R3 = Ransum lengkap + 5% Kompleks I-Minyak Kacang Tanah, …

Isolasi Yeasts Dari Daging Dan Potensinya Sebagai Agen Biopreservasi Dan Pewarna Makanan (Isolation Of Yeasts From Meat And Its Potential As Bio Preservation Agent And Food Coloring), Wendry Setiyadi Putranto, Roostita L. Balia, Obin Rachmawan, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

Yeasts merupakan mikroba potensial untuk dikembangkan sebagai penghasil komponen bioaktif seperti enzim, antimicrobial agent sebagai biopreservasi, serta pengahasil pewarna makanan (pigment).  Penelitian merupakan studi ekploratif potensi yeasts yang diisolasi dari daging ayam dan sapi. Ditemukan isolate yeasts yang memiliki karakteristik spesifik dengan warna merah.  Jumlah total yeasts pada daging ayam 2.105 cfu/gram sedangkan pada daging sapi 1.105 cfu/gram. Hasil pemurnian isolate  diperoleh dua isolate yang memiliki kemampuan daya hambat yang terhadap Pseudomonas aerugenes dan Staphylococus aureus serta tidak meiliki aktivitas penghambatan terhadap Escherichia coli dan Candida albicans. pH akhir media selama fermentasi 25 0C, 48 jam adalah 5,5 dan 5,6 …

Karakteristik Stirred Yoghurt Mangga (Mangifera Indica) Dan Apel (Malus Domestica) Selama Penyimpanan (Characteristics Of Mango (Mangifera Indica) And Apple (Malus Domestica) Sittted Yoghurt During Storage), Eka Wulandari, Wendry Setiyadi Putranto

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan jus buah mangga dan apel terhadap  keasaman, total bakteri dan sineresis stirred yoghurt mangga dan apel yang dihasilkan dan selama proses penyimpanan. Penelitian ini dilakukan dengan tiga perlakuan yaitu stirred yoghurt mangga, stirred yoghurt apel dan kontrol. Stirred yoghurt yang dihasilkan disimpan selama 7 hari. Hasil penelitian menunjukkan stirred yoghurt mangga (210 menit, pH=4,3) dan apel (210 menit, pH=4,2) terkoagulasi lebih cepat dan pada pH yang lebih rendah dibandingkan dengan control (250 menit, pH=4,4). Selama penyimpanan kadar asam laktat dan jumlah bakteri asam laktat lebih tinggi pada stirred yoghurt mangga dan apel dibandingkan …

Deteksi Jumlah Bakteri Total Dan Koliform Pada Lumpur Hasil Ikutan Pembentukan Gasbio Dari Feses Sapi Perah (Detection Total Bacteria Amount And Coliform In Sludge Biogas From Dairy Cattle Feces), Yuli Astuti Hidayati, Ellin Harlia, Eulis Tanti Marlina

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini dilaksanakan di laboratorium Mikrobiologi dan Pengolahan Limbah Fakultas Peternakan universitas padjadjaran. Tujuan penelitian ini adalah untuk mengetahui jumlah total bakteri dan koliform pada lumpur hasil ikutan pembentukan gasbio dari feses sapi perah. Penelitian dilakukan dengan metode eksplorasi pada peternak sapi perah yang membuat instalasi gasbio di desa Haurngombong, Kecamatan Pamulihan-Sumedang. Hasil penelitian menunjukkan jumlah bakteri total adalah 41,82 x 1012 cfu/ml lumpur, sedangkan jumlah 8,23 MPN/ml lumpur. Kata kunci : bakteri total, koliform, lumpur, gasbio, feses sapi perah.

Peran Usaha Perbibitan Dalam Pengembangan Ternak Sapi Perah Di Indonesia (The Role Of Breeding Farm On Dairy Cattle Development In Indonesia), Achmad Firman, Sri Bandiati Komar Prajoga

Jurnal Ilmu Ternak Universitas Padjadjaran

Usaha perbibitan pada peternakan sapi perah memegang peranan penting dalam penyediaan stock bibit sapi perah. Dalam kerangka budidaya sapi perah, perbibitan atau breeding merupakan bagian yang tidak terpisahkan dari siklus feeding, breeding, dan management. Ke tiga faktor tersebut sangat penting dalam usahaternak sapi perah. Tujuan dari kajian ini adalah untuk mengetahui sejauhmana peran perbibitan dan program-program perbibitan dalam penyediaan ternak pengganti, dan untuk mengetahui berapa besar investasi yang diperlukan dalam usaha perbibitan sapi perah. Metode yang dilakukan pada kajian ini adalah kajian pustaka yang dititikberatkan pada usaha perbibitan sapi perah. Hasil penelitian menunjukkan bahwa berdasarkan hasil mapping terhadap peran pelaku …

Pengaruh Penggunaan Asam Sulfat (H2so4) Dan Asam Formiat (Hcooh) Pada Proses Pikel Terhadap Kualitas Kulit Jadi (Leather) Domba Garut (The Effect Of Sulfuric Acid (H2so4) And Formic Acid (Hcooh) In Pickle Process On Garut Sheep Leather Quality), Jajang Gumilar, Wendry Setiyadi Putranto, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan berbagai tingkat asam sulfat (H2So4) dan asam formiat (HCOOH) pada proses pikel  terhadap kualitas kulit jadi (leather) Domba Garut. Kualitas Kulit jadi didasarkan pada kadar keasaman (pH), kadar krom (Cr2O3), dan kadar air (H2O). Penelitian ini dilakukan secara eksperimen menggunakan pola faktorial dengan rancangan dasar rancangan acak lengkap, menggunakan 48 lembar kulit Domba Garut jantan ukuran 90. Kulit-kulit tersebut dibagi kedalam 16 kelompok. Tiap-tiap kelompok diberikan perlakuan penggunaan asam sulfat dan asam formiat. Masing-masing kelompok diulang sebanyak 3 kali ulangan. Hasil penelitian ini menunjukkan bahwa penggunaan berbagai tingkat asam sulfat (H2So4) dan asam …

Pengaruh Suplemen Zn-Proteinat, Cu-Proteinat Dan Kompleks Ca-Minyak Ikan Dalam Ransum Berbasis Pucuk Tebu Amoniasi Terhadap Performans Domba Jantan Persilangan Priangan X Barbados, U. Hidayat Tanuwiria, Budi Ayuningsih

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan mempelajari pengaruh suplemen Zn-Proteinat, Cu-Proteinat dan Ca-Minyak dalam ransum berbasis pucuk tebu diamoniasi terhadap pertumbuhan dan efisiensi ransum pada domba jantan persilangan Priangan x Barbados. Percobaan dilakukan terhadap 5 ekor domba jantan yang sedang tumbuh dengan menggunakan rancangan Bujur Sangkar Latin (BSL) 5x5,  lima macam ransum yaitu R1 = campuran 50% pucuk tebu amoniasi + 50% konsentrat, R2 = R1 + 2% Zn-proteinat, R3 = R1 + 2% Cu-proteinat, R4 = R2 + 2% Cu-proteinat, dan R5 = R4 + Ca-minyak ikan. Hasil yang diperoleh menunjukkan bahwa pemberian Zn-proteinat, Cu-proteinat dan komplek  Ca-minyak tidak mempengaruhi konsumsi ransum, …

Potensi Pakan Serat Dan Daya Dukungnya Terhadap Populasi Ternak Ruminansia Di Wilayah Kabupaten Garut, U. Hidayat Tanuwiria, A. Yulianti, A. Mushawwir

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mempelajari potensi pakan asal limbah tanaman pangan dan daya dukungnya terhadap populasi ternak ruminansia di wilayah kabupaten Garut. Penelitian dilakukan dengan cara survey dan pengumpulan data sekunder.  Hasil penelitian menunjukkan bahwa Potensi pakan asal rumput alam dan jerami asal limbah tanaman pangan di seluruh wilayah kabupaten Garut adalah 414.211,1 ton bahan kering (BK) yang terdiri atas 151.395,6 ton BK asal rumput dan 262.815,5 ton BK asal limbah tanaman pangan. Daya dukung pakan terhadap populasi ternak adalah 124.706 satuan ternak (ST), yang terdiri atas 45.580,5 ST ternak ruminansia kecil dan 79.125,5 ST ternak ruminansia besar.

Kata Kunci …

Potensi Pakan Asal Limbah Tanaman Pangan Dan Daya Dukungnya Terhadap Populasi Ternak Ruminansia Di Wilayah Sumedang (Agriculture By Product As Potential Feed And Its Carrying Capacity In Sumedang), U. Hidayat Tanuwiria, N. Mayasari, A. Yulianti

Jurnal Ilmu Ternak Universitas Padjadjaran

Penelitian ini bertujuan untuk mempelajari potensi pakan asal limbah tanaman pangan dan daya dukungnya terhadap populasi ternak ruminansia di wilayah kabupaten Sumedang. Penelitian dilakukan dengan cara survey dan pengumpulan data sekunder.  Hasil penelitian menunjukkan bahwa Potensi pakan asal rumput alam dan jerami asal limbah tanaman pangan di seluruh wilayah kabupaten Sumedang adalah 284.784,72 ton bahan kering (BK) yang terdiri atas 86.937,65 ton BK asal rumput dan 197.847,07 ton BK asal limbah tanaman pangan. Daya dukung pakan terhadap populasi ternak adalah 85.739,79 satuan ternak (ST), yang terdiri atas 26.174,21 ST ternak ruminansia kecil dan 59.565,58 ST ternak ruminansia besar. Potensi protein …

Pemanfaatan Gelatin Tepung Sagu (Metroxylon Sago) Sebagai Bahan Pakan Ternak Ruminansia (Utilization Of Sago (Metroxylon Sago) Gelatin As Feed Ruminant), Harry Triely Uhi

Jurnal Ilmu Ternak Universitas Padjadjaran

Telah dilakukan penelitian pemanfaatan gelatin sagu sebagai bahan pakan ternak ruminansia. Tujuan penelitian untuk mengetahui suhu, waktu dan ratio volume air yang tepat selama pemanasan sampai terbentuknya gelatinisasi. Justifikasi terjadinya proses gelatinisasi didasarkan atas rusaknya bentuk granula yang dilihat dengan  mikroskop elektron dan mikroskop polarisasi. Perlakuan yang digunakan R1 (Tepung sagu 100 gram + suhu   80 ⁰C); R2 (Tepung sagu 100 gram + suhu   85 ⁰C); R3 (Tepung sagu 100 gram + suhu   90 ⁰C); R4 (Tepung sagu 100 gram + suhu   95 ⁰C); dan R5 (Tepung sagu 100 gram + suhu 100 ⁰C). Parameter …

Pengaruh Tepung Cangkang Rajungan (Portunus Pelagicus) Sebagai Sumber Kitin Dalam Ransum Terhadap Kandungan Lemak Feses Dan Efisiensi Pakan Tikus Putih (Rattus Norvegiccus) Strain Wistar (The Effect Of Crabs Shells (Portunus Pelagicus) As A Source Of Chi, Muhamad Fatah Wiyatna, Irba U. Warsono, Aminuddin Parakkasi

Jurnal Ilmu Ternak Universitas Padjadjaran

Cangkang rajungan mengandung kitin yang persentasenya paling besar berupa polisakarida. Kitin dan derivat-derivatnya telah banyak digunakan dalam berbagai keperluan terutama untuk kesehatan. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh pemberian tepung rajungan sebagai sumber kitin terhadap efisiensi pakan dan kandungan lemak feces tikus putih Rattus norvegicus) strain Wistar. Penelitian menggunakan rancangan acak lengkap pola faktorial 2x5 dengan tiga kali ulangan. Faktor pertama adalah jenis kelamin dan kedua level tepung rajungan yaitu 0%, 5%, 10%, 15%, and 20%. Hasilnya penelitian menunjukkan tidak ada interaksi antara jenis kelamin dengan kandungan teung rajungan dalam ransum. Kandungan lemak dalam feces tertinggi diperoleh …

Pengaruh Lama Penyimpanan Semen Cair Ayam Buras Pada Suhu 5 0c Terhadap Periode Fertil Dan Fertilitas Sperma (The Storage Time Effect Of The Local Chicken Chilled Semen At 5 0c On Fertility And Fertile Period Of Sperm), Nurcholidah Solihati, Ruhijat Idi, Rangga Setiawan, I.Y. Asmara

Jurnal Ilmu Ternak Universitas Padjadjaran

Tujuan penelitian ini untuk mengetahui sejauh mana pengaruh lama penyimpanan semen cair ayam buras pada suhu 5⁰C terhadap periode fertil dan fertilitas sperma.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan setiap perlakuan diulang 6 kali.  Lama penyimpanan yang digunakan dalam penelitian ini adalah penyimpanan 1 jam (0 hari), 24 jam (1 hari) dan 48 jam (2 hari).  Peubah yang diukur adalah periode fertil dan fertilitas sperma. Data yang diperoleh dianalisis dengan analisis sidik ragam, dan untuk mengetahui perbedaan antar perlakuan diuji dengan menggunakan uji jarak berganda Duncan. Hasil penelitian menunjukan bahwa lama penyimpanan semen …

Possible Intervertebral Disc Disease Related To Ventricular Tachycardia In A Labrador Retriever, Nadthagarn Glaewketgarn, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Ventricular arrhythmia (VA) is a common arrhythmia in dogs. Several extracardiac causes such as pain may contribute this condition. This case report describes a 9-year-old neutered male Labrador Retriever with ventricular tachycardia (VT) potentially related to severe neck pain due to intervertebral disc disease (IVDD). The dog was referred with a sign of inability to walk, tachypnea and an irregular heartbeat. Electrocardiography confirmed VT at 160-200 bpm and an increased cardiac troponin I level (35.22 ng/ml). Despite antiarrhythmic treatment, the VT persisted. Cardiac evaluation revealed no structural abnormalities. Interestingly, VT episodes were provoked by cervical palpation and resolved following analgesic …

Optimal Murine Cd4+ T Cell Priming By Mrna-Lipid Nanoparticle Vaccines Requires Endogenous Antigen Processing, Julia E. Rood, Suh Kyung Yoon, Mary K. Heard, Stephen D. Carro, Emma J. Hedgepeth, Mary E. O'Mara, Michael J. Hogan, Nhu Le, Hiromi Muramatsu, Kieu Lam, Petra Schreiner, Coral Kasden, Hansell H. Stedman, Ryan A. Langlois, James Heyes, Norbert Pardi, Laurence C. Eisenlohr

College of Life Sciences Faculty Papers

Lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA vaccines elicit robust CD4+ T cell responses, yet the mechanisms underlying this T cell priming remain unknown. Antigens presented to CD4+ T cells on major histocompatibility complex class II (MHC II) are traditionally acquired by antigen presenting cells (APCs) from extracellular sources. Here we show that vaccine specific CD4+ T cell responses instead rely on antigen directly expressed within APCs, without extracellular transit. Murine APCs treated with mRNA-LNP vaccines activate T cells more efficiently when presenting antigen produced internally, rather than acquired externally. Immunization with mRNA-LNP vaccines engineered to inhibit antigen expression in APCs results …

Institutional Practices Drive Antibiotic Variability In Neonatal Intensive Care Units: Baseline Evidence To Inform National Stewardship Interventions In Oman, Abdullah Alqayoudhi, Manoj Malviya, Sathiya Murthi, Mohammed Rasik Nv, Adil Said Al-Wahaibi, Raya Al-Habsi, Said Al-Balushi, Talal Alwardi, Agha Hatif Shamsi, Halah Bait Raidan, Aamera Al-Majrafi, Preethi Kiran, Eyad Hani Abu Abu Alhaijaa, Kawther Al Amri, Khalfan Al Abdali, Mohammed S. Al Reesi, Nasser Al-Shafouri, Amal Al-Jabri, Sachin Shah, Said Al-Kindi, Zubair H. Aghai, Mohammed Al-Yahmadi, Amal Al-Maani

Department of Pediatrics Faculty Papers

Background: Antibiotic overuse in Neonatal Intensive Care Units (NICUs) is a major contributor to antimicrobial resistance and adverse neonatal outcomes. This study aims to evaluate baseline antibiotic utilization (AU), identify factors influencing variability, and assess the impact of neonatal characteristics and sepsis incidence. Methods: A multicenter retrospective analysis examined AU in seven NICUs from 2019 to 2023, involving 25,532 neonatal admissions during national antibiotic stewardship program implementation. Data encompassed neonatal clinical parameters, sepsis incidence, and AU metrics, including days of therapy (DOT) per 1000 patient-days. Statistical analyses included correlation assessments and multivariate regression to identify determinants of antibiotic use. Results: …

A Programmable Genetic Platform For Engineering Noninvasive Biosensors, Asish N Chacko, Kaamini M Dhanabalan, Jinyang Wan, Roy Chien, Nolan T Anderson, Binzhi Xu, Katie Pham, Ritu Tiwari, Arnab Mukherjee

Faculty, Staff and Student Publications

Creating genetic sensors for noninvasive visualization of biological activities in optically opaque tissues holds immense potential for basic research and the development of genetic and cell-based therapies. Magnetic resonance imaging (MRI) stands out among deep tissue imaging methods for its ability to generate high-resolution images without ionizing radiation. However, the adoption of MRI as a mainstream biomolecular technology has been hindered by the lack of adaptable methods to link molecular events with genetically encodable contrast. Here, we introduce modular aquaporin-based protease-activatable probes for enhanced reporting (MAPPER), a platform for the systematic creation of genetic sensors for MRI. To develop MAPPER, …

Rare Heterozygous De Novo Variants In Rapgef2 Are Associated With A Neurodevelopmental Disorder, Ali H Bereshneh, Kirkland A Wilson, Xueyang Pan, Shabab B Hannan, Megan A Cooper, Jullianne Diaz, Eyby Leon, Tiana M Moses, Mahshid S Azamian, Daryl A Scott, Ping Yee Billie Au, Juan Pablo Appendino, Ingrid E Scheffer, Antony Kaspi, Melanie Bahlo, Michael S Hildebrand, Angela T Morgan, Ekanem Ekure, Joshua M Shulman, Friedhelm Hildebrandt, Jennifer E Posey, Paul Kruszka, Eric Vilain, Shinya Yamamoto, Oguz Kanca, Seth Berger, Hugo J Bellen

Duncan NRI Faculty and Staff Publications

Purpose: RAPGEF2 encodes a guanine nucleotide exchange factor (GEF) that activates small GTPases and has not been linked to a Mendelian disorder. RAPGEF2 is highly intolerant to loss-of-function variants. We report 5 de novo heterozygous variants in RAPGEF2 in unrelated individuals with developmental delay, attention deficit hyperactivity disorder, epilepsy, dysmorphic features, or other manifestations. We used a Drosophila model to assess the functional impact of the identified human variants.

Methods: We generated a Kozak-GAL4 null allele of the Drosophila ortholog of RAPGEF2, PDZ-GEF, and used the allele to determine the gene expression pattern as well as the loss-of-function phenotypes. We …

Glutamatergic Projection Neurons In The Basal Forebrain Underlie Learned Olfactory Associational Valence Assignments, Pey-Shyuan Chin, Zhuokun Ding, Mikhail Kochukov, Snigdha Srivastava, Elizabeth H Moss, Qingchun Tong, Benjamin R Arenkiel

Faculty, Staff and Student Publications

Sensory perception is shaped by experience, giving stimuli behavioral significance. Basal forebrain (BF) cholinergic neurons in mice, which are crucial for arousal and motivation, also regulate sensory processing. Within BF nuclei, glutamatergic (vGlut2BF) neurons receive cholinergic input and modulate behaviors, but their roles in encoding sensory significance remain unclear. Using in vivo calcium imaging, we found that vGlut2BF neurons initially poorly encoded odor identity. However, their response to conditioned odors increased following associative learning, and their population activity more distinctly encoded paired stimuli, reflecting emergent value representation. Furthermore, pairing stimulation or inhibition of vGlut2BF neurons with specific odors altered odor …

Role Of The Etv5/P38 Signaling Axis In Aggressive Thyroid Cancer Cells, Jerry H Houl, Rozita Bagheri-Yarmand, Muthusamy Kunnimalaiyaan, Paola Miranda Mendez, Joseph L Kidd, Ali Dadbin, Andrea Ruiz-Jurado, Parag A Parekh, Ying C Henderson, Nikhil S Chari, Aatish Thennavan, Reid T Powell, Clifford C Stephan, Xiao Zhao, Anastasios Maniakas, Roza Nurieva, Naifa L Busaidy, Maria E Cabanillas, Ramona Dadu, Mark Zafereo, Jennifer R Wang, Stephen Y Lai, Marie-Claude Hofmann

Faculty, Staff and Student Publications

Patients with poorly differentiated thyroid cancer (PDTC) and anaplastic thyroid cancer (ATC) face a much poorer prognosis than those with differentiated thyroid cancers. Around 25% of PDTCs and 35% of ATCs carry the BRAFV600E mutation, which constitutively activates the MAPK pathway, a key driver of cell growth. Although combining BRAF and MEK inhibitors can shrink tumors, resistance often develops. The exact cause of this resistance remains unclear. We previously found that in PDTC and ATC cells, the BRAFV600E mutation is strongly linked to the expression of ETV5, a transcription factor downstream of the MAPK pathway. In the current study, we …

Differential Blood-Brain Barrier Permeability Driven By Asymmetric Hypoperfusion In A Vascular Dementia Model, Mengjun Dai, Kuizhi Qu, Ying Jiang, Song Gao, Yan-Ning Rui, Zhen Xu

Faculty, Staff and Student Publications

Introduction: Blood-brain barrier (BBB) dysfunction has been associated with vascular dementia (VaD). However, the underlying mechanisms causing BBB dysfunction remain unclear, especially regarding cerebral hypoperfusion. This study aimed to investigate the effects of asymmetric hypoperfusion on BBB permeability using a mouse model of VaD.

Methods: Asymmetric bilateral common carotid artery stenosis (ACAS) was induced using ligature rings. BBB integrity was assessed on 3 days post-surgery using Evans blue, IgG, and albumin extravasation. A modified tissue processing protocol optimized endogenous marker detection. Regional and sex-based differences in BBB permeability were analyzed.

Results: Increased BBB permeability was observed in both corpus callosum …

Brain Gains: A Review Of Remediation Treatment Strategies Using Exercise Following Traumatic Brain Injury, Jessica Smith, Kristin Biggins, Loriann Helgeson

The Open Journal of Occupational Therapy

In this Opinions in the Profession article, we explore remediation treatment strategies using exercise for recovery from post-traumatic brain injury and aim to justify the use of such treatment in the occupational therapy profession to increase skills for functional gain. Common treatment strategies used by occupational therapists after brain injury are compensatory strategies, indicating the client learns a new way to perform the task. Though effective, it is also necessary to consider how remediation and neuroplasticity are essential. Teaching new approaches to complete tasks results in functional gains; however, focusing on foundational skills using exercises to relearn motor patterns to …

More Than Covering Ears: A Clinical Inventory For Characterizing Decreased Sound Tolerance (I-Dst) In Autism, Tana B. Carson, Angela Medina, Katherine L. Long, Yuxi Qiu, Monica Noriega

The Open Journal of Occupational Therapy

Background: Decreased sound tolerance (DST) is among the most common and distressing forms of sensory over-responsivity experienced by children with autism spectrum disorders. DST includes hyperacusis and misophonia, among others, and can limit participation in daily activities. Recently, a new screening tool, the Pediatric Hyperacusis and Misophonia Questionnaire (PMHQ-4) has been developed to allow health professionals to better identify and differentiate these conditions using a single assessment. Additional clinical tools are needed to further characterize DST symptoms to facilitate treatment planning after diagnosis.

Methods: In this mixed-methods study n = 15 parents of autistic children 6 to 17 …

Anoikis Resistance And Metastasis Of Ovarian Cancer Can Be Overcome By Cdk8/19 Mediator Kinase Inhibition, Mehri Monavarian, Resha Rajkarnikar, Emily Faith Page, Asha Kumari, Liz Quintero Macias, Felipe Massicano, Nam. Y. Lee, Sarthak Sahoo, Nadine Hempel, Mohit Kumar Jolly, Lara Ianov, Elizabeth Worthey, Abhyudai Singh, Igor Roninson Ph.D., Eugenia Broude Ph.D., Mengqian Chen Ph.D., Karthikeyan Mythreye

Faculty Publications

Anoikis resistance, or evasion of cell death triggered by matrix detachment, is a hallmark of cancer cell survival and metastasis. We showed that repeated exposure to suspension stress followed by recovery under attached conditions leads to development of anoikis resistance. The acquisition of anoikis resistance was associated with enhanced invasion, chemoresistance, and immune evasion in vitro and distant metastasis in vivo. This acquired anoikis resistance was not genetic, persisting for a finite duration without detachment stress, but was sensitive to CDK8/19 mediator kinase inhibition that could also reverse anoikis resistance. Transcriptomic analysis revealed that CDK8/19 kinase inhibition induces bidirectional transcriptional …

“It Seems Like A Never-Ending Job”: Voices Of Female Caregivers Of Older Adults In The Rural Communities, Lalani Nasreen, Bhagyashree Katare, Evans Appiah Osei, Siqi Yang, Sampada Wagle, Julian L. Gallegos, Abidemi Mary Ajuwon

School of Nursing Faculty Publications

Background: Rural female caregivers of older adults face significant caregiving challenges that puts them at high risk for poor self-care and wellbeing. Limited studies have examined the self-care needs of caregivers from a gender equity and social perspective.

Objective: Our study aims to explore the self-care needs and preferences of rural female caregivers and underlying key processes contributing toward their health and well-being. Methods: A qualitative descriptive design was used for the study. A purposive sample of (n = 20) rural female caregivers was obtained. In-depth individual interviews were conducted for data collection. Each interview was about 45- 60 min …

Rab5c Increases Endothelial Release Of Vwf By Regulating Vesicle Trafficking, Paula Reventun, Pablo Toledano-Sanz, Maria Delgado-Marin, Maria Viskadourou, D Brian Foster, Paul S De Vries, Maria Sabater-Lleal, Nunzio Alcharani, Claudia Gonzalez-Cucharero, William O Osburn, Alanna C Morrison, Alisa S Wolberg, Nicholas L Smith, Marios Arvanitis, Charles J Lowenstein

Faculty, Staff and Student Publications

Background: Abnormal levels of VWF (von Willebrand Factor) are a risk factor for venous thromboembolism (VTE) and bleeding. Genome-wide association studies for VWF have identified novel candidate genes that may regulate VWF levels in humans, including RAB5C (RAS-associated protein RAB5C). We hypothesized that RAB5C regulates VWF release from endothelial cells.

Methods: We studied the effect of RAB5C on vesicle trafficking in human endothelial cells. We performed CRISPR (clustered regularly interspaced short palindromic repeats) interference targeting 2 genetic variants linked to altered VWF levels and evaluated RAB5C expression by reverse transcription-quantitative polymerase chain reaction. We silenced RAB5C or overexpressed RAB5C wild-type, …

The Effectiveness Of Aescin Diethylamine Salicylate In Alleviating Peri-Orbital Oedema Ollowing Septorhinoplasty: Follow-Up Study In Basrah, Iraq, Mohannad Mahdi Yaseen, Haider K. Saeed, Ansam Ghazi Abdulwahid

Iraqi National Journal of Medicine

Background: Periorbital oedema following septorhinoplasty is one of the major complications. Various measures have been advocated to alleviate this issue, including the use of a variety of chemical and/or physical materials, such as Aescin. Aim: This study aims to evaluate the role of Aescin in reducing peri-orbital oedema following septorhinoplasty. Methods: Fifty-four patients underwent primary septorhinoplasty at both Syaab and Saddar Teaching Hospitals in Basrah, Iraq, from September 2023 to September 2024. This randomized prospective interventional study collected data from patients using a designed form during the initial interview. The study population was divided into two groups randomly, group A …

Does Valsartan Affect The Cytotoxicity Of Doxorubicin When Used As A Cardioprotective Drug Against Doxorubicin-Induced Cardiotoxicity ?, Shaymaa Fadhil Abbas, Ahmed Bader Abdulwahid, Duha Emad Omran, Nehaya Mnahi Al-Aubody, Mohammad Fawzi Albadran

Iraqi National Journal of Medicine

Background: Doxorubicin is widely used as a chemotherapeutic drug. It has several serious side effects, including cardiotoxicity. Valsartan is an angiotensin II receptor blocker that plays a cardioprotective role against doxorubicin-induced cardiotoxicity. Aim: The aim of our study is to evaluate whether the combination of valsartan and doxorubicin affects the therapeutic efficacy of doxorubicin in treating cancer using an in vitro breast cancer cell line (MCF-7). Methods: Different concentrations of doxorubicin, valsartan, and their combination were tested to detect their cytotoxic effects on the cell line using MTT colorimetric assay method. Three duplicates of each concentration and control were made. …

C Elegans As A Tractable Infection Model For The Emerging Fungal Pathogen Candida Auris, Melissa Martinez, Melissa R Cruz, Danielle A Garsin, Michael C Lorenz

Faculty, Staff and Student Publications

Candida auris is an emerging multidrug-resistant fungal pathogen. The genetic factors contributing to the virulence, drug resistance, and stress-tolerant nature of C. auris are mostly unknown. Additional animal models of virulence are needed, especially those amenable to high-throughput analysis. The nematode Caenorhabditis elegans has been validated as an effective tool for studying multiple fungal and bacterial pathogens. We describe here a C. elegans infection model in which exposure to C. auris is lethal to worms with kinetics similar to killing by Candida albicans; in contrast to C. albicans, C. auris does not form hyphae, indicating distinct virulence mechanisms. …

Bridging Hypoxia And Vision Loss: The Emerging Role Of Connexins In Local And Systemic Eye Diseases, Xianping Zhang, Yalong Cheng, Jean X Jiang, Yuting Li

Faculty, Staff and Student Publications

Hypoxic eye diseases represent a pivotal yet often underappreciated contributor to the onset and progression of many retinal disorders. When hypoxia persists or exceeds the tissue's compensatory capacity, it triggers pathological retinal neovascularization, blood-retinal barrier disruption, and neuronal apoptosis, ultimately resulting in irreversible visual impairment. Connexins (Cxs) form gap junction channels and hemichannels and regulate retinal cell proliferation, differentiation, and survival, thereby playing a central regulatory role in the pathogenesis of hypoxic ocular diseases. In addition to gap junctions, Cx hemichannels promote transmission of molecules between intra- and extracellular environments, further influencing retinal homeostasis under hypoxic stress. This review synthesizes …