Digital Commons Network^™

Breaking Into Hiv-1'S Epigenetic Vault: Cure Strategies To Eliminate The Viral Reservoir, Joanna Jones, Chelsea Gunderson, Brian Wigdahl, Michael Nonnemacher

Kimmel Cancer Center Faculty Papers

Human immunodeficiency virus type 1 (HIV-1) is a retrovirus that integrates into the host cell's DNA as a provirus. Transcription from the provirus is regulated in large part by cellular proteins and epigenetic factors. These may be repressive or permissive to productive infection. The host factors that regulate this balance are therefore attractive targets for HIV-1 therapeutics. Indeed, proviral chromatin is the focus of two of the current HIV-1 cure strategies. "Shock and Kill" uses latency reversal agents to open the provirus's chromatin, promoting high levels of gene expression that induce the killing of infected cells. "Block and Lock" uses …

Glycative Stress Disrupts The Mitochondrial-Lysosome Axis And Promotes Geroconversion In Aging Cardiomyocytes, Diana Bou-Teen, Simonas Valiuska, Elisabet Miro-Casas, Chiara Rubeo, Elena Bonzon-Kulichenko, Zuzana Nichtova, Celia Fernandez-Sanz, Javier Inserte, Antonio Rodriguez-Sinovas, Begoña Benito, Eduard Ródenas-Alesina, Jesús Vázquez, Ignacio Ferreira-González, Marisol Ruiz-Meana

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Aging is a major risk factor for heart failure, yet the molecular mechanisms linking cardiac aging to the inflammatory pathophysiology of heart failure remain elusive. Mitochondrial dysfunction and defective organelle quality control are emerging hallmarks of the aging heart, but their biochemical underpinnings are poorly defined. Using comprehensive glycomics, we found that cardiac mitochondria from physiologically aged mice (≥ 20 months) are the major intracellular reservoirs of advanced glycation end products (AGEs), derived primarily from the chemical attack of some α-oxoaldehydes on proteins. This was associated with mild mitochondrial dysfunction and structural remodeling. Lysosomes in aged hearts were enlarged, more …

Synergistic Microbiome-Peptidome Therapeutics: Exploring Lactobacillus Reuteri Dsmz 17648 And Stem Cell-Derived Peptides For Immunomodulation And Tissue Repair Across Diseases, Hasan M. Agha, Mohammed A. Abdalqader, Abdullah Asad Siddiqui, Shafiq Aazmi, Jantson Hoh, Oh Chin Kin Michelle, Sabaa M. Agha, Amjad Abdulhadi Mohammed, Muneer Mohammed Saleh Alsayadi

AUIQ Complementary Biological System

Chronic inflammatory and degenerative disorders are driven by interconnected immune dysregulation, metabolic instability, epithelial barrier disruption, and impaired tissue repair, limiting the durability of conventional single-target therapies. The global rise in chronic inflammatory, metabolic, and age-related degenerative diseases underscores an urgent need for multi-dimensional therapeutic strategies that move beyond isolated molecular targets. Despite rapid advances in microbiome science and regenerative medicine, these fields have largely evolved in parallel, with limited integration at the translational level. Emerging evidence identifies the gut microbiome as a central regulator of systemic immunometabolic homeostasis through microbial metabolites, barrier integrity modulation, and immune-cell education. In parallel, …

Energetic Heterogeneity In Retinal Ganglion Cells: Role Of Neuronal Activity And Implications For Resilience, Zelun Wang

Arts & Sciences Graduate Student Theses and Dissertations

Retinal ganglion cells (RGCs) form the only connection between the eye and the brain via their axons in the optic nerve. In diseases such as glaucoma, RGCs undergo irreversible neurodegeneration leading to vision loss. RGCs consist of multiple types differing in morphology, gene expression, and signaling properties. Moreover, these types exhibit differential survival after axon injury. What cellular properties vary between RGC types that could underlie their heterogeneous response to neurodegeneration? Given that energy metabolism is tightly optimized for the homeostatic and active functions of different neurons, physiologically distinct RGC types likely exhibit divergent energetic characteristics. Furthermore, energy metabolism is …

No Effect In Plasma Corticosterone Levels After Observation Of Diseased Conspecifics, B. M. Hardy, M. Chang, S. Mccallum, S. Santangelo, Patricia C. Lopes

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Organisms commonly respond to stressors in their environment by increasing the secretion of corticosterone (CORT). While increased CORT is acknowledged as a response to predation risk by organisms inhabiting a landscape of fear, new research raises the potential for changes in CORT as a response to the risk of infection by organisms in their environment. An increase in CORT of a healthy, uninfected individual in response to their perception of the risk of infection in their environment could change their susceptibility to infection, highlighting a significant role for CORT to alter host–pathogen dynamics. To investigate the effects of infection risk …

Mist: A Hierarchical Bayesian Framework For Detecting Differential Dna Methylation Dynamics In Single-Cell Data, Daoyu Duan, Wenjing Ma, Wen Tang, Hao Wu, Liangliang Zhang, Hao Feng

Faculty, Staff and Student Publications

Recent advancements in single-cell DNA methylation (scDNAm) sequencing technologies have enabled the profiling of epigenetic landscapes at unprecedented resolution, offering insights into cellular heterogeneity, differentiation and evolution. Trajectory inference, which orders cells along pseudotime, allows researchers to track genomics changes across continuous cell states and identify key loci exhibiting differential methylation. However, no methods currently exist to model methylation changes along pseudotime in scDNAm data. Here, we present a hierarchical Bayesian framework for scDNAm data analysis. Our method, named mist (methylation inference for single-cell along trajectory), models stage-specific biological variations, identifies genomic features with significant methylation changes along pseudotime, and …

Pkmζ-Kibra Interactions, Molecular Turnover, And Memory, Changchi Hsieh, David A Cano, Panayiotis Tsokas, James E Cottrell, André Antonio Fenton, Harel Shouval, Todd Charlton Sacktor

Faculty, Staff and Student Publications

How can the molecules that strengthen synaptic connections maintain memory in the face of molecular turnover? Our previous work showed that persistent interaction between the postsynaptic scaffolding protein, KIBRA, and the autonomously active PKC isoform, PKMζ, is crucial for maintaining synaptic long-term potentiation (LTP) and memory lasting at least a month. This duration is longer than the lifespans of individual KIBRA and PKMζ molecules. Biophysical modeling of the interaction suggests oligomers of KIBRA-PKMζ dimers, but not individual dimers or monomers, can overcome molecular turnover by continuously incorporating newly synthesized KIBRA and PKMζ, replacing those that have degraded. Here we used …

Megakaryocyte And Platelet Thrombospondin-1 Regulates Matrix Remodeling By Stabilizing Basement Membrane Col6a1 In Lung Injury, Hernán F Peñaloza, Atish Gheware, Ananya Gupta, Donovan Watza, Anupam Kumari, Zeyu Xiong, Pooja Bhojraj, Jae-Sung Kim, Bo-Ram Jin, Kyunghee Choi, Matthew R Rosengart, Jaehyung Cho, Janet S Lee, Et Al.

2020-Current year OA Pubs

An early event after lung injury is extracellular matrix (ECM) remodeling and the formation of a provisional matrix. While megakaryocytes and platelets (Mgk/plt) play important roles in hemostasis, their impact on the matrix in lung injury is not fully understood. Using lung intravital microscopy, 3D large area scanning with multiphoton confocal hybrid imaging, and label-free quantitative proteomics, the matricellular protein thrombospondin-1 (TSP1) arising from Mgk/plt protects the lung from alveolar injury. Mgk/plt cell-specific Thbs1 knockout (cKO) mice show increased alveolar barrier disruption with exaggerated neutrophil-mediated injury. The cKO mice exhibit striking extracellular matrix re-organization with reduction in basement membrane matrix …

In Vivo Hsc Gene Therapy Enables Sustained Ecd4-Ig Expression For Siv Prevention, Chang Li, Anna K Anderson, Anne-Sophie Kuhlmann, Veronica Nelson, Audrey Germond, Hongjie Wang, Aphrodite Georgakopoulou, Sucheol Gil, Jasmin Martinez-Reyes, Andrew Riker, Shruthi Shankar Raman, Jiho Kim, Philip Ng, Donna Palmer, Michael D Alpert, Nickolas Skamangas, Charles Bailey, Tianling Ou, Christine M Fennessey, Michael Farzan, Keith R Jerome, Brandon F Keele, Hans-Peter Kiem, André Lieber, John K Bui

Faculty, Staff and Students Publications

We aim to develop an in vivo hematopoietic stem cell (HSC) gene therapy approach for the prevention and control of HIV-1 infection. Toward this goal, we engineered helper-dependent adenovirus (HDAd) 6/3+ vectors to directly transduce HSCs in vivo, enabling progeny cells to secrete eCD4-Ig, a decoy protein that broadly neutralizes HIV/simian immunodeficiency virus (SIV) isolates by mimicking the primary viral receptor CD4 and coreceptors such as CCR5. In rhesus macaques, the HDAd 6/3+ platform achieved long-term expression of an enhanced eCD4-Ig variant (“eCD4-Ig-Emm06”) that retained potent neutralization efficacy in vivo. Transduced HSCs differentiated into lymphoid and myeloid lineages …

Investigating Corollaries Between Streptococcus Gallolyticus Subsp. Gallolyticus Virulence And Colorectal Cancer, Tyler G. Myers

Pursuit - The Journal of Undergraduate Research at The University of Tennessee

Streptococcus gallolyticus subsp. gallolyticus (Sgg) is a gram-positive, non-beta-hemolytic bacterium that belongs to the Lancefield group D of streptococcal pathogens. For the past several decades, associations between Sgg bacteremia and colorectal cancer (CRC) have been apparent, with gastroenterologists routinely recommending colonoscopies for positive cases of Sgg in either blood or stool samples. However, current research has not wholly established whether Sgg acts as a causative agent of CRC or if pre-existing cancers make the lumen of the colon more hospitable to its colonization. Regardless, Sgg has been implicated in the modulation of host inflammatory pathways and subsequent remodeling …

Lsu Health New Orleans School Of Dentistry Welcomes Esperanza Academy For Give Kids A Smile Day

New Orleans Dental Association News

LSU Health New Orleans School of Dentistry (LSUSD) reinforced its long standing commitment to community oral health by welcoming approximately 120 first, second, and third graders from Esperanza Academy for this year’s Give Kids a Smile Day—a national initiative dedicated to expanding access to dental care and oral health education for children

The article showcases the event services, activities, contributors, and fun photos full of costumes.

Noda News March 2026

New Orleans Dental Association News

New Orleans Dental Association (NODA) News includes regular columns with regional news impacting the New Orleans membership including: columns from the President and Executive Director, feature articles relating to the field of dentistry, association events and upcoming activities, component news, continuing education opportunities, and classifieds.

Optimizing Healthcare Utilization: Distinguishing Between Emergency And Urgent Care, Madilyn Calkins, Molly Dupar, Wintana Gherie, Marcin Janczak, Angela Lee, Lena Zhang

Nursing Leadership in Community Engagement Projects

Optimizing Healthcare Utilization: Distinguishing Between Emergency and Urgent Care

Over the past two months, our group of six nursing students worked with a local transitional housing program for families. Per the facility report, a large portion of the program’s current residents are immigrants and have had little to no exposure to the American healthcare system and their options for care. The issue we targeted for our project is the increased number of 911 calls and emergency room (ER) visits for non-emergent healthcare concerns by our target population. By creating education materials on self-triaging, ER vs. Urgent Care services, and how …

Molecular Insights Into The Regulation Of Gnptαβ By Lyset, Xi Yang, Balraj Doray, Danielle Henn, Varsha Venkatarangan, Benjamin C Jennings, Zhongzheng Dong, Jiaxuan Liang, Weichao Zhang, Bokai Zhang, Linchen Yu, Liang Chen, Stuart Kornfeld, Ming Li

2020-Current year OA Pubs

In vertebrates, newly synthesized lysosomal enzymes traffic to lysosomes through the mannose-6-phosphate (M6P) pathway. The Golgi membrane protein LYSET was recently discovered to regulate lysosome biogenesis by controlling the level of GlcNAc-1-phosphotransferase (GNPT). However, its working mechanism remained unclear. In this study, we demonstrate that LYSET is a two-transmembrane protein essential for GNPT stability, cleavage by Site-1 Protease (S1P), and enzymatic activity. We reconcile conflicting models by showing that LYSET enhances GNPT cleavage and prevents its mislocalization to lysosomes for degradation. We further establish that LYSET achieves this by interacting with GOLPH3 and retromer complexes to anchor the LYSET-GNPT complex …

Histopathological Study On The Effect Of Antioxidants (Vitamin E And Selenium) In Hepatotoxicity Induced By Lead Acetate In Rats, Adnan W. Al-Bideri

Al-Qadisiyah Medical Journal

The study was carried out to evaluate histopathological changes inrats liver induced by different doses and durations of lead acetate toxicity and to investigate the therapeutic effects of vitamin E and Selenium together against lead poisoning.Thirty two adult male rats (Rattus rattus), randomized into four groups (n=8) were used for this study. In group I rats received only drinking water as control while in   group II , III and IV rats were given o.5%,1%,1.5% lead acetate respectively in drinking water for ten weeks. Two animals from each group were sacrificed and and dissected after five weeks and ten weeks of …

A Multi-State Look Into Early Intervention Speech-Language Pathologists’ Confidence Identifying And Diagnosing Autism, Allison Gladfelter, Adelaide Parr, Anna Cannone, Sarah O'Connor

CISLL Publications

Purpose: Although autism can be reliably diagnosed by 18 months of age, long wait times and limited access to qualified providers prevent families from obtaining timely diagnostic services. Trained speech-language pathologists (SLPs) are qualified to diagnose autism, ideally as part of a multidisciplinary team. SLPs working on early intervention (EI) teams are well-situated to help close this diagnostic wait time gap. The purpose of this survey study was to explore EI SLPs’ experiences serving autistic children, confidence in identifying and diagnosing autism, and their perceptions of barriers or facilitators to diagnostic confidence and wait times.

Method: 287 EI SLPs from …

Differential Contributions Of Clpx And Clpp To Pulmonary Virulence In Classical And Hypervirulent Klebsiella Pneumoniae, Nathan M Lin, Emily C Marino, Jordan M Schlotmann, David A Rosen

2020-Current year OA Pubs

No abstract provided.

Underexplored Maternal Microbiomes: Immune, Metabolic, And Microbial Pathways Shaping Pregnancy Outcomes, Rafael Tomoya Michita, Nicole Jimenez, Melissa M Herbst-Kralovetz, Indira U Mysorekar

Faculty, Staff and Students Publications

Maternal microbial ecosystems play critical roles in shaping reproductive physiology and pregnancy outcomes. During the pre-conception and prenatal periods, these communities modulate maternal physiology by regulating immune tolerance, nutrient metabolism, and susceptibility to pregnancy complications such as preterm birth, hypertensive disorders, and gestational diabetes. While the gut microbiota has been extensively studied, the roles of cervicovaginal, urinary, respiratory, oral, and upper reproductive tract microbiomes remain less clear. In this minireview, we synthesize current knowledge on these underexplored maternal microbiomes, with an emphasis on the cervicovaginal and urinary microbiota and their interactions with the placenta and fetus. We discuss cross-niche microbial …

Dual Phagocytosis-Checkpoint Blockade Revitalizes Immune Surveillance In Mouse Models Of Glioblastoma, Jonghoon Ha, Yifan Wang, Yifan Ma, Annette Wu, Shiyan Dong, Seong Dong Jeong, Jared L Edwards, Mengyu Chang, Yen-Tzu Chang, Xiaotian Wang, Maurice Dufilho, Michelle Najarro Torres, Shannon Mccabe, Weiye Deng, Adam J Grippin, Jeffrey S Weinberg, Shaan M Raza, Franco Demonte, Ian E Mccutcheon, Sujit Prabhu, Sherise D Ferguson, Chibawanye I Ene, Kadir Akdemir, Sreyashi Basu, Sonali Jindal, Benjamin R Schrank, Jian Hu, Sangeeta Goswami, Vinay K Puduvalli, Frederick F Lang, Kristin Huntoon, Padmanee Sharma, Betty Y S Kim, Wen Jiang

Faculty, Staff and Student Publications

Macrophage-mediated phagocytosis of tumor cells elicits potent antitumor immunity. Nonetheless, sole-blockade of the anti-phagocytosis molecule CD47 has yielded insufficient therapeutic outcomes. Here, we report that glioblastoma (GBM) cells expressed abundant levels of phagocytosis checkpoint CD24. We further show that dual blockade of CD24 and CD47 synergistically enhances the pro-phagocytic activity of macrophages, thereby improving tumor antigen cross-presentation and activating the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway. This innate immune activation facilitates T cell infiltration into tumors and sensitizes tumors to anti-PD1 therapy, improving survival outcomes in murine GBM models, including immunosuppressive tumors reflecting human GBM-like features. Thus, our …

Patient And Healthcare Professionals' Perceptions Of Educational Tools To Reduce Urine Culture Contamination In Outpatient Clinics: A Qualitative Study., Ashley Collazo, Trenton M Haltom, Johanan Luna Rodriguez, Kiara Olmeda, Azalia Mancera, Fabrizia Faustinella, Michael K Paasche-Orlow, Zach Landis-Lewis, Roger Zoorob, Barbara W Trautner, Larissa Grigoryan

2020-Current year OA Pubs

OBJECTIVE: Iteratively develop educational tools (instructional video and flyer) to improve midstream clean catch (MSCC) urine sample collection using patient and healthcare professionals' input.

DESIGN: Multi-method qualitative study.

SETTING: Outpatient clinics in Houston, Texas, United States.

PARTICIPANTS: Adult patients recruited from public and private clinics (

METHODS: Twelve patient interviews and three focus groups with HCPs (May 2024-November 2024). Interviews discussed patient experiences using the educational tools to guide urine specimen collection. Focus groups elicited HCPs' perspectives on the comprehensibility and utility of the tools in their respective clinics. We identified themes using directed content analysis.

RESULTS: We garnered insight …

Microrna-625-3p Increases Chemosensitivity In Ovarian Cancer Cells Through Decreasing Ssx2ip-Mediated Cisplatin Export In Extracellular Vesicles, Chi-Lam Au-Yeung, Tetsushi Tsuruga, Marina A Talor, Yadira J Pacheco, Guangan He, Zahid H Siddik, Byeong J Cha, Suet-Ying Kwan, Kwong-Kwok Wong, Kay-Pong Yip, Samuel C Mok

Faculty, Staff and Student Publications

Introduction: Advanced-stage high-grade serous ovarian cancer (HGSC) is a disease that is difficult to manage due to its heterogeneous clinical behavior. No reliable prediction of response to chemotherapy is currently available and the overall survival rate remains poor. Herein, we sought to determine the molecular mechanisms by which microRNAs (miRNAs) confer chemoresistance in ovarian cancer and demonstrate the efficacy of targeting miRNAs to sensitize HGSC to cisplatin treatment.

Methods: Next-generation miRNA sequencing was performed using microdissected HGSC specimens to identify an miRNA signature for intrinsic chemoresistance, and miR-625-3p was selected for further study. The effects of miR-625-3p on cisplatin sensitivity …

Waste-To-Energy Innovations And Advances In Hydrothermal Carbonization, Microwave, And Pyrolysis Processes: A Review, Ali Mohammed Saleh, Noah Mohammed Saleh, Mahmod A. Abdulqader, Hadi Hamdi Mahdi, Jafar Keighobadi, Omar K. Ahmed, Khalil Farhan Yassin, Azil Bahari Bin Alias, Omar Abed Habeeb, Ibrahim Awad Mohammed

AUIQ Complementary Biological System

The accelerating global generation of municipal solid waste, coupled with escalating energy demands, presents one of the most pressing sustainability challenges of the 21st century. Conventional waste disposal pathways, such as landfilling and incineration, exacerbate environmental degradation and resource loss. In response, thermochemical technologies—specifically hydrothermal carbonization (HTC), microwave-assisted processing, and pyrolysis—have emerged as promising strategies for waste valorization and renewable energy recovery. This review critically examines recent advancements in these technologies, emphasizing their roles in converting diverse waste streams into high-value energy carriers and carbon-rich materials. Comparative analysis reveals that HTC operates efficiently under subcritical water conditions, producing hydrochar with …

A Novel Laparoscopic Renal Denervation System In A Preclinical Swine Model, Linwei Zhao, Wei Yang, Binbin Zhu, Yahui Liu, Peng Tian, Xinyu Guo, Yang Dong, Zhiqiang Fan, Huiping Li, Yu Feng, Lijie Zhu, Minfu Bai, Qiuping Zhao, Xingkun Zhang, Chenchen Si, Qianqian Cheng, Gan-Xin Yan, Chuanyu Gao

Department of Medicine Faculty Papers

Laparoscopic renal denervation (RDN) represents a specialized approach for hypertension, particularly when conventional methods face limitations. While catheter-based RDN has demonstrated significant efficacy in recent large-scale clinical trials and has been integrated into international guidelines, its effectiveness can be constrained by complex renal anatomy or incomplete ablation. We developed a novel laparoscopic RDN system, comprising an integrated radiofrequency (RF) clamp, RF generator, and cooling pump, to achieve more controlled adventitial ablation. This study aims to evaluate the safety, feasibility, and optimal parameters of this new system in a preclinical swine model. Sixteen pigs were divided into an immediate group ( …

Three-Dimensional Printing Of The Epineurium For Peripheral Nerve Repair: A Comprehensive Review Of Novel Scaffolds For Nerve Conduits, Alynah J. Adams, Iulianna C. Taritsa, Kaavian Shariati, Aaron Dadzie, Jose A. Foppiani, Maria Jose Escobar-Domingo, Daniela Lee, Angelica Hernandez-Alvarez, Kirsten Schuster, Helen Xun

School of Medicine Publications

Background: Nerve conduits are used to bridge peripheral nerve defects caused by trauma, iatrogenic injury, or oncologic disruption. Three-dimensional (3D) biomimetic scaffolds for peripheral nerve regeneration have advanced significantly in recent years, driven by improvements in printing technology and neuronal seeding techniques. We report on published designer conduits that can recreate the epineurium, a critical yet challenging-to-manufacture feature of nerve tissue.

Methods: A medical librarian conducted a literature search for our systematic review on EMBASE, Web of Science, and PUBMED, following PRISMA guidelines, for articles from January 2010 to January 2026 for the systematic review. Descriptive statistical analysis was performed …

Crossing The Finish Line Towards A Disease-Modifying Treatment For Angelman Syndrome, Matthew C Judson, Jason J Yi, Et Al.

2020-Current year OA Pubs

Recent progress in the development of genetic therapies promises that impactful treatments for single-gene neurodevelopmental disorders are imminent. But can derailed neurodevelopmental processes be mended after broken genes are replaced or otherwise restored? The results of ongoing clinical trials for Angelman syndrome will soon yield answers to this pressing question, yet the trials face significant obstacles. Here we identify insights needed to aid the quest for a disease-modifying Angelman syndrome therapy, which could serve as a roadmap for the expeditious development of genetic therapies for other single-gene neurodevelopmental disorders.

Development Of A Sustainable Blended Food Aid Product Using Local Ingredients For Nutritionally Vulnerable Populations In Haiti, Sherlie Jean Louis Dulience, Rachel Zimmerman, Michelle Dorce, Ilana Seff, Andrea Spray Bulungu, Patricia Kohl, Carolyn Lesorogol, Bazelais Dulience, Lora Iannotti

2020-Current year OA Pubs

OBJECTIVES: This study aimed to: (1) identify optimal blended food recipe options using local flours; (2) assess behaviors, attitudes, and practices around the use of blended foods among nutritionally vulnerable groups in Haiti; and (3) evaluate the nutrient composition of prototype blended food product relative to nutrient requirements for vulnerable populations.

METHODS: Blended food recipes made from local flours were identified through matrix scoring and stakeholder consensus. Focus groups (n = 7) assessed behaviors and attitudes toward blended foods. Prototype recipes were selected based on matrix scoring, program participant feedback, and feasibility of bringing to scale. Nutrient composition of the …

Translation Of Expanded Cgg Repeats In Lrp12 Associated Oculopharyngodistal Myopathy, Chengcheng Li, Jil A Daw, Sara K Pittman, Connor J Maltby, Hidetoshi Sakurai, Peter K Todd, Conrad C Weihl

2020-Current year OA Pubs

Oculopharyngodistal myopathy (OPDM) is characterized by ptosis, ophthalmoparesis, dysphagia, and distal weakness. Myopathological features include rimmed vacuoles and intranuclear inclusions. OPDM is associated with a pathogenic CGG repeat expansions in the 5'UTR of LRP12, NOTCH2NLC, GIPC1, RILPL1 and ABCD3. Translation of the repeat in the glycine reading frame has been demonstrated for expansions in FMR1, NOTCH2NLC and GIPC1. To assess for a similar phenomenon with LRP12, we expressed normal or expanded CGG repeats in the context of the 5'UTR of LRP12, upstream of a green fluorescent protein (GFP) in the three repeat reading frames. Repeat dependent translation occurs exclusively in …

A New Chromosome-Level Genome Assembly For Western Painted Turtle Chrysemys Picta Bellii, A Model For Extreme Physiological Adaptations, Junhao Chen, John R Bermingham Jr, Patrick Minx, Milinn Kremitzky, Zhenguo Lin, Daniel E Warren

2020-Current year OA Pubs

The western painted turtle, Chrysemys picta bellii, has the greatest tolerance to anoxia of any tetrapod studied to date. These turtles reside in the northern United States and southern Canada, and survive months of anoxia while submerged in ice-locked ponds and bogs. Reference genomes provide an important resource for elucidating the molecular bases for such unique physiological traits. The initial reference genome for this species, published in 2013, is highly fragmented, thereby limiting downstream analyses and biological interpretation. We created a new and improved assembly by combining PacBio HiFi, 10 × Genomics Chromium, Hi-C sequence data and Bionano optical mapping …

The Development And Evaluation Of A Comprehensive Well-Being Instrument For Health Professionals - The Corewellness Index, Nicholas E. Anton, Lauren Ball, Jeffrey S. Levy, Nicky Beaudoin, Catherine F. Pipas

Journal of Wellness

Introduction: Healthcare worker wellness has received increasing attention from medical educators and healthcare systems given its importance to provider longevity and patient care. Wellness is multidimensional, and very few instruments evaluate all domains of wellness. Our team developed a comprehensive measure to evaluate the multi-dimensional nature of wellness. The purposes of this study were divided into four phases: phase 1) detail the development, design, and initial dissemination of the instrument, phase 2) collect feasibility and normative data for a diverse sample of healthcare workers, phase 3) evaluate the internal reliability of the measure and its component domains, and phase 4) …

Anhedonia Underlies The Association Between Childhood Unpredictability And Adult Ptsd Symptoms: Evidence From Three Independent Longitudinal Cohorts, Christopher Hunt, Laura M. Glynn, Elysia Poggi Davis, Tallie Z. Baram, Caroline M. Nievergelt, Giovanni Castillo, Christian Cortes, Christine Y. Chang, Dewleen Baker, Victoria B. Risbrough

Psychology Faculty Articles and Research

Background

Unpredictability in the child’s environment has recently emerged as a significant and unique form of early life adversity (ELA). Cross-sectional studies have linked childhood unpredictability with increased post-traumatic stress disorder (PTSD) symptoms in adults; however, no prospective studies have tested the link between childhood unpredictability and PTSD risk in later life, nor what processes, such as increased anhedonia symptoms, might mediate such risk. Here, we leveraged three distinct prospective, longitudinal cohorts to test the hypothesis that unpredictability during childhood contributes to adult PTSD via worsening anhedonia symptoms.

Methods

Participants were male service members (n=314), adult females (n=170), and adolescents …