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Pre-Molten, Wet, And Dry Molten Globules En Route To The Functional State Of Proteins, Munishwar Nath Gupta, Vladimir N. Uversky
Pre-Molten, Wet, And Dry Molten Globules En Route To The Functional State Of Proteins, Munishwar Nath Gupta, Vladimir N. Uversky
Molecular Medicine Faculty Publications
Transitions between the unfolded and native states of the ordered globular proteins are accompanied by the accumulation of several intermediates, such as pre-molten globules, wet molten globules, and dry molten globules. Structurally equivalent conformations can serve as native functional states of intrinsically disordered proteins. This overview captures the characteristics and importance of these molten globules in both structured and intrinsically disordered proteins. It also discusses examples of engineered molten globules. The formation of these intermediates under conditions of macromolecular crowding and their interactions with nanomaterials are also reviewed.
Liaisons Dangereuses: Intrinsic Disorder In Cellular Proteins Recruited To Viral Infection-Related Biocondensates, Greta Bianchi, Stefania Brocca, Sonia Longhi, Vladimir N. Uversky
Liaisons Dangereuses: Intrinsic Disorder In Cellular Proteins Recruited To Viral Infection-Related Biocondensates, Greta Bianchi, Stefania Brocca, Sonia Longhi, Vladimir N. Uversky
Molecular Medicine Faculty Publications
Liquid–liquid phase separation (LLPS) is responsible for the formation of so-called membrane-less organelles (MLOs) that are essential for the spatio-temporal organization of the cell. Intrinsically disordered proteins (IDPs) or regions (IDRs), either alone or in conjunction with nucleic acids, are involved in the formation of these intracellular condensates. Notably, viruses exploit LLPS at their own benefit to form viral replication compartments. Beyond giving rise to biomolecular condensates, viral proteins are also known to partition into cellular MLOs, thus raising the question as to whether these cellular phase-separating proteins are drivers of LLPS or behave as clients/regulators. Here, we focus on …