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Role Of Insulin-Like Growth Factor-I In The Autocrine Regulation Of Cell Growth In Tt Human Medullary Thyroid Carcinoma Cells, Kuo-Pao P. Yang, Naguib A. Samaan, Yi-Fan Liang, Socorro G. Castillo

Henry Ford Hospital Medical Journal

Since the TT human medullary thyroid carcinoma cell line required fewer exogenous growth factors (serum), we investigated whether this line has an autocrine mechanism by examining the effects of antibodies directed toward insulin-like growth factor I (IGF-I) and its receptor on TT cell growth in serum-free conditions. Treating cells with anti-IGF-I antibody for four days reduced the cell number by more than 50% compared with a nonimmune IgG control. Furthermore, a monoclonal antibody to the IGF-I receptor suppressed DNA synthesis when determined by a [³H]thymidine incorporation assay. Exogenous IGF-I (20 ng/mL) stimulated [³H]thymidine incorporation in serum-free …

Biologic And Cytogenetic Characterization Of Three Human Medullary Thyroid Carcinomas In Culture, R. Pfragner, G. Wirnsberger, A. Behmel, B. Niederle, F. Längle, R. Roka, A. Mandl, P. Pürstner, J. Auner, F. Tatzber

Henry Ford Hospital Medical Journal

Neuroendocrine features and cytogenetic abnormalities of one continuous cell line (MTC-SK) and two long-term cultures (GER, STAH) derived from three sporadic cases of human medullary thyroid carcinomas (MTCs) were studied. Specific neuroendocrine markers (NSE, chromogranins, calcitonin, calcitonin gene-related peptide) were identified by electron microscopy and immunocytochemistry. In situ hybridochemistry and Northern blot analysis confirmed endocrine activity. Cytogenetic studies of the cell line MTC-SK revealed three consistent marker chromosomes, t(3;10), 11p+, and 22p+. Cells of long-term cultures GER and STAH exhibited a consistent translocation t(2;18), a trisomy 7, and two consistent marker chromosomes der3 and 5p+, respectively. Recently, we have isolated …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Fixed-Ratio Size As A Determinant Of The Development Of Tolerance To Morphine, Mark J. Nickel

Masters Theses

The acute and chronic effects of morphine were examined in pigeons exposed to a multiple schedule with fixed ratio 5, 25, and 125 components. Acute exposure to morphine (0.56-10.0 mg/kg) resulted in rate reductions under each component when the dose was 1 mg/kg or higher. With chronic exposure to 5.6 mg/kg, tolerance to the rate-reducing effects of morphine was evident under each fixed ratio component. The development of tolerance was determined to some extent by fixed-ratio size, a result similar to earlier findings with cocaine.

Development Of Periapical Lesions In Immunosuppressed Rats, Phillip A. Waterman Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

The pathogenesis of periapical lesions is the interaction of the oral flora with the existing host defenses. The role of bacteria has been established in endodontic disease but the contribution of the host defenses in periapical lesion formation is less clear. The purpose of this study was to compare periapical lesion development in immunosuppressed rats with that in normal rats. Thirty-five, eight-week old Sprague-Dawley rats were equally divided into seven groups. Groups 1, 2, and 3 were given weekly injections of Cytoxan, an immunosuppressive agent. Groups 4, 5, and 6 received no immunosuppressive therapy. Group 7 served as a control, …

Digest Of The Philadelphia College Of Osteopathic Medicine (Summer 1992), Philadelphia College Of Osteopathic Medicine

Digest

The Summer 1992 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• OMC's Common Vision: Quality Medical Education and Patient Care: President’s Perspective
• Sen. Arlen Specter Honored at PCOM's 101st Commencement
• Biggest Expansion Project Underway: Parkview Looks Forward To New Medical Office Building
• Individualized Giving Provides a Personal Touch
• PCOM Alumni Experience Excitement and Anxiety of Authorship
• Joining the Ranks of Alexander Graham Bell: National Inventors Hall of Fame Honors PCOM Professor
• A Healthy Way to Heal: PCOM Alumnus Bicycles his Way to Work
• On Campus
• Class Acts
• In Memoriam
• Coming Events

Department Of Radiology-Annual Report-July 1, 1991 To June 30, 1992, David C. Levin

Department of Radiology Administrative Documents and Reports

Department of Radiology Annual Report, July 1, 1991 to June 30, 1992. Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, United States. 72 pages.

Cortisol In The Ovine Fetus : Its Regulation And Role In Parturition, Ede Marie Apostolakis

Loma Linda University Electronic Theses, Dissertations & Projects

In fetal sheep, circulating cortisol increase during late gestation to abruptly rise 72 hours preceding birth. Although the surge in cortisol is thought to initiate the endocrine cascade which triggers parturition, it is not known what drives the prepartum rise in fetal cortisol. The first study tested the hypothesis that basal cortisol secretion is regulated by adrenocorticotropin (ACTH) which in turn, is controlled by arginine vasopressin (AVP). The fetus challenged with AVP increased plasma ACTH and cortisol concentrations. Pretreatment with an AVP V₁ receptor antagonist blocked AVP-induced plasma ACTH cortisol but failed to alter basal ACTH or cortisol. To …

Neutron Fluence And Dose From A Varian Clinic 1800 Accelerator, Richard A. Jackson

Theses and Dissertations

The goals of this study were (1) to verify calibration factors determined by previous experiment for relating counts from activated gold foils to neutron fluences, and (2) to use the method to determine neutron fluences under various operating conditions of the Varian Clinac 1800 (18-MeV) electron accelerator at the WPAFB Medical Center. For comparison similar measurements were made of the CGM Saturne 1 at Kettering Memorial Hospital, Dayton, Ohio. Cadmium-difference pairs in a standard graphite pile were employed to verify the counting efficiency of the Geiger-Mueller beta counting system used to count the foil activity. They were also used to …

Alternative Splicing Of Human Prostaglandin G/H Synthase Mrna And Evidence Of Differential Regulation Of The Resulting Transcripts By Transforming Growth Factor Beta 1, Interleukin 1 Beta, And Tumor Necrosis Factor Alpha., Arturo Diaz, Anthony M. Reginato, Sergio A. Jimenez

Department of Medicine Faculty Papers

Prostaglandin G/H synthase (PGG/HS) is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins and thromboxanes. We screened a human lung fibroblast cDNA library with an ovine PGG/HS cDNA and isolated a 2.3-kilobase clone (HCO-T9). Sequence analysis of this clone showed that (a) it contained the entire translated region of PGG/HS and (b) it displayed an in-frame splicing of the last 111 base pairs encoded by exon 9, which resulted in the elimination of the N-glycosylation site at residue 409. Polymerase chain reaction amplification with specific oligonucleotides of reverse-transcribed mRNA from diverse human tissues and cultured cells yielded …

The Ratio Of Heinz Body Formation In Different Hemoglobin Zurich Subjects, Yenya Hu

Masters Theses & Specialist Projects

Hemoglobin Zurich is a hemoglobin anomaly that results when one amino acid (histidine) is substituted by arginine at position 63 in the beta chain of hemoglobin molecules [β 63 His—Arg]. When Hemoglobin Zurich individuals are exposed to sulfonamide medication, their hemoglobins denature and subsequently form Heinz bodies which attach to the surface of the plasma membrane.

Four Hemoglobin Zurich family members were the subjects of the current study. They included a splenectomized female subject, non-splenectomized female and male subjects, and a non-splenectomized female member without Hemoglobin Zurich as the control. The results collaborate that splenectomy increases the number of erythrocytes …

Cytolytic T Lymphocytes Specific For Tumors And Infected Cells From Mice With A Retrovirus-Induced Immunodeficiency Syndrome., Jennifer G. Erbe, Kathy A. Green, Karen M. Crassi, Herbert C. Morse, W R. Green

Dartmouth Scholarship

LP-BM5 retrovirus complex-infected C57BL/6 mice develop immunodeficiency, somewhat analogous to AIDS, termed murine AIDS (MAIDS). After secondary stimulation with syngeneic B-cell lymphomas from LP-BM5-infected mice, C57BL/6 mice produced vigorous CD8+ cytotoxic T lymphocytes specific for MAIDS-associated tumors. An anti-LP-BM5 specificity was suggested because spleen and lymph node cells from LP-BM5-infected mice served as target cells in competition assays, and cells from LP-BM5, but not ecotropic, virus-infected mice functioned as secondary in vitro stimulators to generate cytotoxic T lymphocytes to MAIDS tumors.

Ms211: Texas Hadassah Medical Research Foundation (Thmrf) Board Announcing Foundation's Goals, Armin Weinberg

Works on Radiation Effects: 1990-2020

This looks like draft Press Release piece of the announced the affiliation with The Methodist Hospital Health Care Network. Talks about initial plans to organize a conference, list of additional member of the effort, including a Tx Congressman Martin Frost as honorary member, and a report from an Israeli scientist from Hadassah Zeev Weshler on the issue facing them with immigrants to Israel from the contaminated areas. See more at Armin Weinberg, PhD Papers and its finding aid.

An Update On The Role Of Ploidy In Prostate Carcinoma, Jill M. Peters-Gee

Henry Ford Hospital Medical Journal

One of the characteristic features of prostate carcinoma is its marked variation in biologic behavior. DNA quantitation has been studied in prostate carcinoma using a variety of techniques. Evaluation of tumor ploidy suggests that this may be the best predictor of the biologic behavior of prostate cancer in individual patients. This comprehensive review addresses the current studies, stage by stage, to clarify the clinical significance of these findings.

Primary Papillary Serous Carcinoma Of The Peritoneum: A Case Of Complete Remission Of Bulky Peritoneal Disease After Chemotherapy, Walid A. Saleh, Cosmas J. M. Van De Ven, Wooshin Kim

Henry Ford Hospital Medical Journal

Peritoneal papillary serous carcinoma is a rare tumor that involves the surface of the pelvic and/or abdominal peritoneum. Long-term survival among patients with this tumor has been rare. Most patients with this cancer have been treated with debulking surgery and postoperative chemotherapy. A case of incompletely resected peritoneal papillary serous carcinoma with a complete response to cisplatin-based chemotherapy is reported. Subsequent laparotomy revealed no residual tumor. This case suggests that primary chemotherapy may be successful in treating unresectable primary papillary serous tumors of the peritoneum.

The Current Role Of Prostatic Acid Phosphatase And Prostate-Specific Antigen In The Management Of Prostate Cancer, Sugandh D. Shetty, Joseph C. Cerny

Henry Ford Hospital Medical Journal

No abstract provided.

Adenocarcinoma Of The Prostate: Overview Ii, Joseph C. Cerny

Henry Ford Hospital Medical Journal

No abstract provided.

The Epidemiology Of Prostate Cancer In Black Men, David A. Burks, Ray H. Littleton

Henry Ford Hospital Medical Journal

Data on the epidemiology of prostate cancer from the 1930s to the present document a dramatic racial difference in incidence, survival, and mortality rates in American men. American black men have the highest incidence and mortality rates of prostate cancer in the world. Survival data have been related to access to medical care, genetic and environmental factors, and cultural differences, including diet and social habits. Most reports present conflicting data with no clear positive correlations, and conclusions are often speculative. Better controlled, prospective studies of epidemiologic variables and a comprehensive genetic evaluation of black families with prostate cancer are needed …

Maximal Androgen Ablation: A Review, Brian J. Miles, Joseph Babiarz

Henry Ford Hospital Medical Journal

Primary management of advanced (stage D) adenocarcinoma of the prostate is androgen ablation. Since this principle was discovered in the early 1940s, therapeutic alternatives and "progress" have centered around different ways to obtain castrate levels of androgens. The role of adrenal androgens in supporting prostate or prostatic cancer growth has been debated for decades and until recently was believed to be minimal. In the 1980s the concept of maximum androgen suppression, involving both the testes and adrenal glands, was reintroduced with some investigators claiming exceptional results. We review studies that have examined this concept, with emphasis on the largest trial …

Presentation And Evaluation Of Patients With Epidermoid Head And Neck Cancers, Michael S. Benninger

Henry Ford Hospital Medical Journal

Epidermoid cancer of the head and neck occurs most frequently in individuals with predisposing factors for neoplastic development. Primarily these include exposure to smoking and alcohol, although other predisposing factors may he present. Site-speciflc symptoms along with a history of predisposing environmental exposure can direct physicians to suspect head and neck cancer. Endoscopy and ancillary testing are performed for tumor staging and subsequent treatment planning.

Ms211: Background Examples Of Literature Searches On Topics Of Interest, Armin Weinberg

Works on Radiation Effects: 1990-2020

A zip file of various literature searches & some resources related to our work related to exposure after the Chernobyl accident and as we began looking at helping in Semey Kazakhstan----a collection of literature reviews on various topics we were interested in... eg. establishing a registry of those exposed for longterm follow-up, what we knew about certain areas like genetics and some resources like "A Guide to Environmental Resources" on the Internet by Carol Briggs-Erickson and Toni Murphy which could be found on the Internet and was written to be used by researchers, environmentalists, teachers and any person who is …

The Effect Of Body Position Changes On Arterial Oxygen Saturation In Obesity As Measured Noninvasively By Pulse Oximetry, Anna Marie Angela Klosterman

Theses and Dissertations

This study utilized a quasi-experimental pretest-posttest design that compared the effect of body position changes on arterial oxygen saturation in obesity as measured noninvasively by pulse oximetry. The null hypothesis, that there would be no difference between oxygen saturation values measured by pulse oximetry with position change from sitting to supine in obese subjects as compared to nonobese subjects, was tested. Fourteen obese, American Society of Anesthesiologist (ASA) physical class status II subjects and 14 nonobese ASA physical class status I subjects were included in the sample population. Thirteen of the obese subjects were further classified as morbidly obese. After …

Oncolog, Volume 37, Issue 03, July-September 1992, Scott M. Lippman Md, Mark A. Schusterman M.D., Eva Singletary Md, Merrick I. Ross M.D., Daryl Ogden Ms

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Retinoic acid/interferon combination shows promising response rates
• Cross-matching test helps find donors for patients in dire need of platelets
• Silicone gel-filled implants: Women should have the option to choose

Oncolog, Volume 37, Issue 02, April-June 1992, Ralph S. Freedman Md, Phd, Bartlett D. Moore Iii,Phd, Nicholas H.A. Terry Phd

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• The primacy of patient welfare
• Potential doubling time of tumors may be the key to accurate prognosis, appropriate treatment
• Cognitive deficits in survivors of childhood cancers

Oncolog, Volume 37, Issue 01, January-March 1992, Roman Perez-Soler Md, Waldemar Priebe Md, Michael Needle, Sidney Wallace Md

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Clinician and chemist forge new approach to liposome research
• Computerized network enhances interdisdplinary patient management
• Neurofibromatosis: a new clinic brings new opportunities

Insulin-Like Growth Factor Ii Acts Through An Endogenous Growth Pathway Regulated By Imprinting In Early Mouse Embryos, Daniel A. Rappolee, Karin S. Sturm, Ole Behrendtsen, Gilbert A. Schultz, Roger A. Pedersen, Zena Werb

Department of Obstetrics and Gynecology Research Publications

We present evidence that insulin-like growth factor II (IGF-II) mediates growth in early mouse embryos and forms a pathway in which imprinted genes influence development during preimplantation stages, mRNA and protein for IGF-II were expressed in preimplantation mouse embryos, but the related factors IGF-I and insulin were not. IGF-I and insulin receptors and the IGF-II/mannose-6-phosphate receptor were expressed. Exogenous IGF-II or IGF-I increased the cell number in cultured blastocysts, but a mutant form of IGF-II that strongly binds only the IGF-II receptor did not. Reduction of IGF-II expression by antisense IGF-II oligonucleotides decreased the rate of progression to the blastocyst …

The Use Of Self Blood Glucose Monitoring And Its Relationship To Effective Management For People With Insulin Dependent Diabetes Mellitus, Maureen Unsworth

Theses : Honours

It is believed that people who effectively self manage their own diabetes will achieve higher levels of health and well being (Pender, 1987). Although diabetes education has increased rapidly in the last 10 years, the emphasis has been placed on increasing people's knowledge of diabetes as a medical condition, which does not necessarily ensure effective self management of diabetes. Previous research suggests that self blood glucose monitoring (SBOM) is an effective tool for promoting the use of blood glucose levels as a basis for initiating behaviour changes related to eating, exercise and insulin dosage. Yet, while most people with diabetes …

Heterogeneous Macromolecular Composition Of Renal Basement Membranes In Health, Diabetes And Glomerulonephritis, Douglas K. Brees

MUSC Theses and Dissertations

Although basement membranes are ubiquitous structures throughout the body, basement membranes have distinct compositions that are specific to their location. This basement membrane heterogeneity may, in part, reflect functional differences among various basement membranes. We examined basement membrane heterogeneity in normal, healthy mouse kidneys to assess the similarities and differences between glomerular and tubular basement membrane composition. It was demonstrated that mouse glomerular and tubular basement membrane share similar compositions but differ with respect to specific amounts of some components. In diabetes mellitus and passive Heymann nephritis (PHN), damage to the glomerular barrier occurs and is accompanied by an increase …

Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Replication of human adenovirus type 12 DNA is blocked in abortively infected baby hamster kidney cells. The activity and accumulation of adenovirus 12 DNA polymerase is equivalent in infected hamster and human cell extracts. However, the accumulation of adenovirus type 12 DNA-binding protein is approximately 120-fold lower in extracts from infected hamster cells when compared to infected permissive human cells. This difference in accumulation is not because of replication of viral DNA during productive infection, since this difference is observed in the presence of hydroxyurea. The DNA-binding protein from infected hamster cells retains the ability to bind denatured DNA-cellulose. An …

Regulation Of Transforming Growth Factor-Beta 1 Gene Expression By Glucocorticoids In Normal Human T Lymphocytes., O. Ayanlarbatuman, A. P. Ferrero, Arturo Diaz, Sergio A. Jimenez

Department of Medicine Faculty Papers

Glucocorticoids (GC) modulate immune function in a number of ways, including suppression of T cell proliferation and other IL-2-mediated T cell functions. These inhibitory effects are similar to those induced by transforming growth factor-beta 1 (TGF-beta 1), a cytokine with potent T cell inhibiting activities. We examined the hypothesis that GC effects may be at least partially achieved through modulation of the expression of the TGF-beta 1 gene in activated T cells. Normal T cells were cultured with or without purified phytohemagglutinin (PHA-p) and 4 beta-phorbol 12-myristate 13-acetate (PMA) in the presence or absence of the synthetic GC, dexamethasone (100-200 …