Digital Commons Network^™

Moraxella (Branhamella) Catarrhalis: A Molecular Epidemiology Study, Lyndell R. Gill

Electronic Theses and Dissertations

Moraxella (Branhamella) catarrhalis is the third-most-frequently isolated microorganism associated with acute exacerbations of chronic bronchitis in patients during their stay at the Mountain Home VA Medical Center (MHVAMC). In order to develop a practical, epidemiologically-meaningful typing method for M. (B.) catarrhalis, we tested two methods based on analysis of chromosomal DNA for typeability, reproducibility, and ability to differentiate between unrelated strains (discriminatory power, D). M. (B.) catarrhalis isolants from MHVAMC from 7/1/87-6/30/88 were grown overnight in broth and embedded in agarose. DNA was isolated by standard methods. The DNA was subjected to: (1) restriction endonuclease digestion (with either Bgl II …

Indomethacin Reduces Splenic Red Pulp Macrophage Populations In Female New Zealand White Rabbits, Thane S. Thurmond

Electronic Theses and Dissertations

In an effort to elucidate the mechanism by which indomethacin (IN) attenuates the stimulatory effect of estradiol (E$\sb2$) on rabbit splenic red pulp macrophages (RPM), thirty-nine female New Zealand White rabbits were divided into 10 groups: ovariectomized (OVX), OVX/IN at 0.1 and 5.0 mg/kg body weight (bw)/day; sham OVX (SOVX), SOVX/IN at 0.1 and 5.0 mg/kg bw/day; OVX/25 mg E2, OVX/25 mg E$\sb2$/IN at 0.1 and 5.0 mg/kg bw/day; intact Control. Quantitative changes in RPM population in response to the treatments were measured using a 0 to 4 histologic grading scale. Estradiol treatment resulted in increased RPM grade when compared …

Increased Alpha 1(I) Procollagen Gene Expression In Tight Skin (Tsk) Mice Myocardial Fibroblasts Is Due To A Reduced Interaction Of A Negative Regulatory Sequence With Ap-1 Transcription Factor., Neena Philips, Reza I. Bashey, Sergio A. Jimenez

Department of Medicine Faculty Papers

The TSK mouse, a model of fibrosis, displays exaggerated connective tissue accumulation in skin and visceral organs including the heart. To study the mechanisms of myocardial fibrosis in TSK mice, we established several strains of TSK mice myocardial fibroblasts in culture and examined the regulation of collagen gene expression in these cells. These strains displayed increased collagen gene expression in comparison with myocardial fibroblasts established from normal mice. On an average, the TSK myocardial fibroblast cultures showed a 4-fold increase in collagen synthesis and 4.4- and 3.6-fold increases, respectively, in alpha 1(I) and alpha 1(III) collagen mRNA steady state levels. …

Vital Signs, Spring 1995, Boonshoft School Of Medicine

Vital Signs

A twenty four page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, alumni profiles, class notes, and more.

Mathematical Models Of Chemotherapy, John Carl Panetta

Mathematics & Statistics Theses & Dissertations

Several mathematical models are developed to describe the effects of chemotherapy on both cancerous and normal tissue. Each model is defined by either a single homogeneous equation or a system of heterogeneous equations which describe the states of the normal and/or cancer cells. Periodic terms are added to model the effects of the chemotherapy. What we obtain are regions, in parameter space (dose and period), of acceptable drug regimens.

The models take into account various aspects of chemotherapy. These include, interactions between the cancer and normal tissue, cell specific chemotherapeutic drug, the use of non-constant parameters to aid in modeling …

Polyclonal Mycobacterium Avium Infections In Patients With Aids: Variations In Antimicrobial Susceptibilities Of Different Strains Of M. Avium Isolated From The Same Patient., C Fordham Von Reyn, Nicholas J. Jacobs, Robert D. Arbeit, Joe N. Maslow, S Niemczyk

Dartmouth Scholarship

Broth microdilution MICs were determined for pairs of strains isolated from five AIDS patients with polyclonal Mycobacterium avium infection. Four (80%) of the five patients were infected simultaneously with strains having different antimicrobial susceptibility patterns. These findings have implications for the interpretation of susceptibility data in M. avium prophylaxis and treatment trials.

Sustained Release Drug Delivery Devices, Thomas J. Smith, Paul Ashton, Paul A. Pearson

Ophthalmology and Visual Science Faculty Patents

A method and device for treating a mammalian organism to obtain a desired local or systemic physiological or pharmacological effect is provided. The method includes administering a sustained release drug delivery system to a mammalian organism in need of such treatment at an area wherein release of an effective agent is desired and allowing the effective agent to pass through the device in a controlled manner. The device includes an inner core or reservoir comprising the effective agent; a first coating layer, which is essentially impermeable to the passage of the effective agent; and a second coating layer, which is …

Health Professions Division Catalog 1995-1996, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Feasability Of Preventing Encrustation Of Urinary Catheters, J. M. Bibby, A. J. Cox, D. W. L. Hukins

Cells and Materials

Colonization of urinary catheters by bacteria which produce urease leads to an increase in urine pH, followed by deposition of the minerals struvite and hydroxyapatite. Adhesion of these encrusting deposits can be reduced, but not prevented, by using catheters with a smooth surface finish. Chemical methods for preventing encrustation are not completely satisfactory. A better way of preventing encrustation would be to prevent colonization of the catheter by bacteria. This might be achieved by controlled release of antimicrobial agents directly into the urine from the catheter itself. Preliminary experiments have demonstrated the feasibility of controlled release from solid silicone. However, …

Thyroid Hormone Influence On Oxygen Consumption Rates, Body Mass, And Lipid Metabolism In Mice With Noninsulin Dependent Diabetes Mellitus, Catherine Renee Clark

Theses Digitization Project

No abstract provided.

Bone Absorptiometry In Metabolic Bone Disease: Baseline Values And Long-Term Treatment With Calcitriol (Post-Menopausal Osteoporosis Versus Osteomalacia), A. Caniggia, B. Frediani

Cells and Materials

Total body bone absorptiometry reveals low mineral density in both postmenopausal osteoporosis and osteomalacia patients. The method was used to investigate the effect of calcitriol administration on patients suffering from one of these conditions. In osteomalacia, the administration of calcitriol resulted in a dramatic improvement in bone mineral density (sometimes up to 50% in 12 months), indicating the rapid mineralization of previously uncalcified bone tissue as a result of the normalization of the Ca x P product. In osteoporosis a similar treatment was seen to halt the progressive decrease in bone mineral levels and sometimes resulted in minor increases in …

Some Observations On The Structure Of Encrusting Biofilms Of Proteus Mirabilis On Urethral Catheters, C. Winters, D. J. Stickler, N. S. Howe, T. J. Williams, N. Wilkinson, C. J. Buckley

Cells and Materials

A physical model of the bladder has been used to study the process of urinary catheter encrustation. Techniques have been devised for the preparation of sections through these encrustations in situ on the luminal surfaces of catheters and for mapping the distribution of calcium and magnesium in the biofilms. Transmission electron microscopy on these sections showed struvite-like crystals lying in the matrix and in direct contact with the catheter surface. Calcium phosphate ("bioapatite") was distributed throughout the film and many of these amorphous particles appear to have cells at their cores. Freeze-substituted sections of biofilms also showed electron dense materials …

Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore

Nebraska Center for Virology: Faculty Publications

In vitro neurovirological studies of viral infectivity or viral gene expression may be confounded by the mulHple neural cell types and/or fibrob last contamination present in early passage cultures prepared from dissociated human central nervous system (eNS) tissue. We have developed highly enriched astrocyte cultures for neurovirological study by culturing in a serum-free defined medium, 816, supplemented with basic fibroblast growth factor (FGF-2). Subculture in this medium selects against fibroblast proliferation and favors sustained proliferation of a highly enriched glial fibrillary acidic protein (GFAP)-positive cell population. These astrocytes support productive replication of cytomegalovirus (CMV) and transient expression of transfected CMVand …

Loss Of The Tumor Suppressor Genes P53 And Brca1 In Prophylactic Mastectomy Cancer Patients, Garrett Griffith Losh

Presidential Scholars Theses (1990 – 2006)

More importantly, this study has shown that prophylactic mastectomy surgery is a viable and worthwhile treatment to prevent breast cancer recurrence. It is hoped that this information will increase the knowledge of breast cancer. For the same reason, this paper has attempted to explain what cancer is while describing how the genetics of a person cause or contribute to its development. Most of all, it is hoped that this paper will emphasize the severity of this disease in the United States today and tomorrow .

Cerebral Vascular Accidents And Therapeutic Treatments, Allison L. Hartman

Presidential Scholars Theses (1990 – 2006)

A cerebral vascular accident (CVA) is commonly referred to as a stroke. Stroke is the third leading cause of death and the number one cause of serious disability in the United States. Approximately 550,000 people suffer a stroke each year; 150,000 of these result in death. Each stroke is unique. After a stroke, some victims will suffer severe deficits and/or permanent disability for the remainder of their lives. Yet other victims may suffer only mild, temporary deficits and may recover completely. Currently over three million Americans are living with the effects of stroke . Most of these individuals have been …

Oncolog, Volume 40, Number 02, April-June 1995, Margaret E. Goode, Vickie` J. Williams, Sunita Patterson

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Creative strategies key in fight against liver cancer
• Getting patients home and back to their routines more quickly
• Teaching physicians how to prevent cancer

Oncolog, Volume 40, Number 03, July-September 1995, Kathryn L. Hale, Vickie` J. Williams, Sunita Patterson

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• M. D. Anderson leads the fight against bone pain from metastatic cancer
• New computer program enhances the surgeon-patient consultation
• Bladder cancer: molecular markers may guide treatment

Oncolog, Volume 40, Number 04, October-December 1995, Kathryn L. Hale, Vickie J. Williams

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Psychiatry at M. D. Anderson: Helping patients learn to live with cancer
• Technology and cooperation powerful tools in locating genes
• Second opinion clinic helps patients get the best treatment

Vital Signs, Winter 1995, Boonshoft School Of Medicine

Vital Signs

A twenty three page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, alumni profiles, class notes, and more.

Localisation Of The Gene For A Novel Form Of Charcot-Marie-Tooth Disease In An Isolated Population, Kaite Honeyman

Theses : Honours

Localising the gene for a previously undescribed autosomal recessive form of CMT involved the use of a relatively new approach to rapid genome screening based on the identification of segments which are inherited identical by descent (IBD) from common founding ancestors. It is most feasible for populations which have been founded relatively recently (say less than 25 generations) and which have remained relatively isolated either geographically or culturally. The method is not suitable for highly inbred populations, that is with first and second cousin matings, as many segments will be inherited by chance. It appears to be a suitable screening …

Perceptions Of Control And Satisfaction With Hospital Birth Experiences For First-Time Mothers, Clelia Tedeschi

Theses : Honours

Satisfaction with childbirth is associated with women's future emotional wellbeing. This study examined whether first- time mother's antenatal expectations, postnatal evaluations of control during labour and delivery, and the discrepancy between expectations and evaluations were significant predictors of women's satisfaction with their childbirth experiences. The effect of medical interventions (e.g., obstetrical interventions and pain relief medication) on women's perceptions of control and satisfaction was also examined. The purposive sample of eighty first-time mothers, mean age 26 years (excluding women who had caesareans) delivered at the public hospital where they attended antenatal classes. During their fourth antenatal class women completed a …

Long Term Restoration Of Peak Torque, Endurance And Muscle Activation Post Anterior Cruciate Ligament Reconstruction, Darryl Alan Turner

Theses : Honours

In recent years, Accelerated Rehabilitation (A.R.) protocols have been used following Anterior Cruciate Ligament (A.C.L.) reconstructions, the longer term effects of these programs are seldom analysed. This study examined musculoskeletal mechanisms that might affect long term functional restoration, the control subjects (n = 19) of mixed age and gender, had no previous knee deficiencies and were recreationally fit. The experimental subjects (n = 17) had undergone intra-articular A.C.L reconstruction, using bone patellar-tendon bone grafts performed by the same surgeon and had also participated in the A.R. protocol, similar to that described by Shelbourne and Nitz (1990). The experimental group attended …

A Logistic Model Of Periodic Chemotherapy, J. C. Panetta

Mathematics & Statistics Faculty Publications

A logistic differential equation with a time-varying periodic parameter is used to model the growth of cells, in particular cancer cells, in the presences of chemotherapeutic drugs. The chemotherapeutic effects are modeled by a periodic parameter that modifies the growth rate of the cell tissue. A negative growth rate represents the detrimental effects of the drugs. A simple criterion is obtained for the behavior of the chemotherapy.

Phase Ii Trial Of Murine Monoclonal Antibody D612 Combined With Recombinant Human Monocyte Colony-Stimulating Factor (Rhm-Csf) In Patients With Metastatic Gastrointestinal Cancer, Mansoor Saleh, Michael Khazaeli, Richard Wheeler, Pat Bucy, Tiepe Liu, Michael Everson, David Mun, Jeff Schlom

Haematology and Oncology, East Africa

In a Phase II study, 14 patients with metastatic gastrointestinal cancer received the mAb D612 (40 mg/m2, days 4, 7, and 11) in combination with recombinant human monocyte colony-stimulating factor [(rhM-CSF) 80 ug/kg/days 1-14]. The combined treatment was well tolerated and resulted in characteristic biological activity associated with each of the agents. Thus, 10 of 14 patients experienced D612-associated secretory diarrhea, which responded to the prostaglandin inhibitor Indomethacin in 5 of 7 patients. rhM-CSF therapy was associated with peripheral mono- cytosis (peak absolute monocyte count, 1444 ±394/mm3) and thrombocytopenia (nadir count, 78 ±10/nim '). Monocyte surface marker analysis revealed a …

Cd16+ Monocytes In Patients With Cancer: Spontaneous Elevation And Pharmacologic Induction By Recombinant Human Macrophage Colony- Stimulating Factor, Mansoor Saleh, Goldman Samuel, Lobuglio Albert, Beall Arthur, Sabio Hernan, Mccord Melissa, Minasian Lori, Alpaugh Katherine, Weiner Louis, Munn David

Haematology and Oncology, East Africa

The small subset of circulating monocytes that express the maturation- associated CD16 antigen has recently been reported to be elevated in patients with bacterial sepsis. We now show that this novel CD16+ monocyte population is also spontaneously expanded in patients with cancer. We studied 14 patients with metastatic gastrointestinal carcinoma enrolled in a clinical trial of recombinant human macrophage colony-stimulating factor (rhMCSF) plus monoclonal antibody D612. We found that before any cytokine treatment, 12 of 14 patients constitutively displayed significant elevations in both the percentage and the absolute number of CD16+ monocytes, as compared with both normal subjects and ill …

Digest Of The Philadelphia College Of Osteopathic Medicine (Fall 1994), Philadelphia College Of Osteopathic Medicine

Digest

The Fall 1994 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• ABC’s of Medical Education
• New Curriculum: Making Students Better Doctors
• First OMM Fellowship Recipients Named
• Herbert Lotman: Chairman of the Board
• PCOM Profile: Alexander E. Rodi, DO
• PCOM Annual Report 1994: Building for the Future
• Academic Update
• The PCOM Mission
• Master Facilities Plan
• PCOM Financial Review
• Officers of the College
• PCOM Board of Trustees
• PCOM Alumni Recognized at ACOS Convention
• PCOM Celebrates National Primary Care Day
• PCOM Golf Classic
• PCOM Appoints Senior Vice President
• Evening of Art Raises Funds for PCOM Health …

Vital Signs, Fall 1994, Boonshoft School Of Medicine

Vital Signs

A twenty page newsletter created by the Boonshoft School of Medicine to document the current affairs of the school. This issue includes a variety of feature articles, alumni profiles, class notes, and more.

Symptoms Six Weeks After Covid-19 Are Reduced Among Us Health Care Personnel Receiving Additional Vaccine Doses During The Omicron Period, December 2021-April 2022., Nicholas M. Mohr, Ian D. Plumb, Eliezer Santos León, Malea Pinckney, Karisa K. Harland, Anusha Krishnadasan, Karin F. Hoth, Fernand Rwamwejo, John P. Haran, Melissa Briggs-Hagen, Eric Kontowicz, David A. Talan

Jefferson Hospital Staff Papers and Presentations

BACKGROUND: The objective of this study was to test the hypothesis that subsequent doses of the coronavirus disease 2019 (COVID-19) vaccine are associated with lower incidence of COVID-19-like symptoms at 6 weeks after infection.

METHODS: This study was a case-control analysis of health care personnel in an ongoing multicenter COVID-19 vaccine effectiveness study. We enrolled participants at the time of COVID-19-like symptoms between December 19, 2021, and April 27, 2022, which corresponded to the early Omicron-predominant period after original monovalent severe acute respiratory syndrome coronavirus 2 additional vaccination doses became available. Our outcome was self-reported symptoms completed 6 weeks after …

Epidermal Growth Factor Coordinately Regulates The Expression Of Prostaglandin G/H Synthase And Cytosolic Phospholipase A2 Genes In Embryonic Mouse Cells., Kenneth P. Chepenik, Arturo Diaz, Sergio A. Jimenez

Department of Medicine Faculty Papers

Confluent, primary cultures of mouse embryo palate mesenchyme (MEPM) cells are refractory to activation of phospholipase A2 (PLA2) by the calcium ionophore A23187. However, treatment of these cultures with epidermal growth factor (EGF) permits the cells to activate PLA2 in response to A23187. We have developed this finding by exploring molecular mechanisms by which growth factors modulate mobilization and metabolism of arachidonic acid. We found chronic treatment (> 6 h) of confluent MEPM cells with EGF (a) increases their ability to metabolize exogenous arachidonic acid to prostaglandin E2 (PGE2) and (b) stimulated constitutive expression of activities of PLA2 and cyclooxygenase …

Stab Wounds To Rat Brains : Demyelination, Remyelination And The Cellular Response, Particularly Of Oligodendrocytes, Xie Dangci

Loma Linda University Electronic Theses, Dissertations & Projects

The present body of knowledge concerning the results of central nervous system injury is incomplete and controversial. The role of oligodendrocytes, the cells involved in phagocytosis, the stages of demyelination, and the evidence for remyelination are of interest. Following stab wounds to rat brain cortex, corpus callosum and hippocampus, the animals were examined at 4,7,11,18,23,29,45,60 and 70 days postoperation (dpo). To help in the identification of cells immunocytochemical markers for astrocytes and immature oligodendrocytes were used. These were labels for glial fibrillary acidic protein (GFAP) and myelin/oligodendrocyte specific protein (MOSP) respectively. Light and electron microscopy were correlated using conventionally prepared …