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Insulin-Like Growth Factor Ii Signaling In Neoplastic Proliferation Is Blocked By Transgenic Expression Of The Metalloproteinase Inhibitor Timp-1, David C. Martin, John L. Fowlkes, Bojana Babic, Rama Khokha

Pediatrics Faculty Publications

Insulin-like growth factor (IGF) II is overexpressed in many human cancers and is reactivated by, and crucial for viral oncogene (SV40 T antigen, [TAg])-induced tumorigenesis in several tumor models. Using a double transgenic murine hepatic tumor model, we demonstrate that tissue inhibitor of metalloproteinase 1 (TIMP-1) blocks liver hyperplasia during tumor development, despite TAg-mediated reactivation of IGF-II. Because the activity of IGFs is controlled by IGF-binding proteins (IGFBPs), we investigated whether TIMP-1 overexpression altered the IGFBP status in the transgenic liver. Ligand blotting showed that IGFBP-3 protein levels were increased in TIMP-1-overexpressing double transgenic littermates, whereas IGFBP-3 mRNA levels were …

Global Cns Gene Transfer For A Childhood Neurogenetic Enzyme Deficiency: Canavan Disease., Paola Leone, Christopher G Janson, Scott J Mcphee, Matthew J During

Department of Neurosurgery Faculty Papers

The neurogenetic prototypic disease on which we chose to test our gene therapy strategy is Canavan disease (CD). CD is an autosomal recessive leukodystrophy associated with spongiform degeneration of the brain. At present the disease is uniformly fatal in affected probands. CD is characterized by mutations in the aspartoacylase (ASPA) gene, resulting in loss of enzyme activity. In this review, recent evidence is summarized on the etiology and possible treatments for CD. In particular, we discuss two gene delivery systems representing recent advances in both viral and liposome technology: a novel cationic liposome-polymer-DNA (LPD) complex, DCChol/DOPE-protamine, as well as recombinant …

Signaling Mechanisms For Muscarinic Receptor-Mediated Coronary Vasoconstriction In Isolated Rat Hearts, Yi Zhang

Electronic Theses and Dissertations

The signaling mechanisms for muscarinic receptor-mediated vasoconstriction in coronary resistance arteries were studied in KCl-arrested isolated rat hearts perfused at a constant flow rate. The cholinergic agonists acetylcholine and bethanechol were given by bolus injection or constant infusion. The coronary vascular resistance was monitored by measuring the changes in perfusion pressure. The selective muscarinic agonist bethanechol caused a similar vasoconstrictor response as ACh, but with less potency and efficacy. Bolus injection of bethanechol evoked a phasic vasoconstriction in a dose-dependent manner, while infusion of bethanechol evoked a tonic vasoconstriction without producing tachyphylaxis. Coronary vascular responses to bethanechol were further examined …

Laser Skin Graft Harvesting Apparatus And Related Method, David S. Kirn

Surgery Faculty Patents

A laser dermatome is disclosed for harvesting and meshing grafts of skin. A roller driven by a motor lifts the leading edge of a portion of skin for cutting by a beam of light generated by a laser. Adhesive tape attached to the roller aids in lifting the portion of skin and is further utilized to secure the severed portion of skin to the roller. A stage mounted atop an axle supports the laser or a beam director. The movement of the stage is controlled by second and third motors which drive the stage along or rotate it about the …

Digest Of The Philadelphia College Of Osteopathic Medicine (Summer 1999), Philadelphia College Of Osteopathic Medicine

Digest

The Summer 1999 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Opening Remarks
• PCOM Updates
• Update on Clinical Education
• Board Profile
• Alumni Board Profile
• Convention Highlights
• Class Notes
• In Memoriam
• Coming Events

Interruption Of Multiple Cellular Processes In Ht-29 Epithelial Cells By Pseudomonas Aeruginosa Exoenzyme S, Joan C. Olson, Jennifer E. Fraylick, Eileen M. Mcguffie, Katherine M. Dolan, Timothy L. Yahr, Dara W. Frank, Timothy S. Vincent

Dartmouth Scholarship

Exoenzyme S (ExoS), an ADP-ribosylating enzyme produced by the opportunistic pathogen Pseudomonas aeruginosa, is directly translocated into eukaryotic cells by bacterial contact. Within the cell, ExoS ADP-ribosylates the cell signaling protein Ras and causes inhibition of DNA synthesis and alterations in cytoskeletal structure. To further understand the interrelationship of the different cellular effects of ExoS, functional analyses were performed on HT-29 epithelial cells after exposure to ExoS-producing P. aeruginosa 388 and the non-ExoS-producing strain 388DeltaS. Two different mechanisms of morphological alteration were identified: (i) a more-transient and less-severe cell rounding caused by the non-ExoS-producing strain 388DeltaS and (ii) a more-severe, …

The Accuracy Of Nitrous Oxide Passive Dosimeters As Compared With An Infra-Red Spectrographic Reference Method, Susan Allen

Community & Environmental Health Theses & Dissertations

Nitrous oxide is a gas often used in dental, surgical and veterinary operations as an anesthetic agent. Employees stationed in proximity of fugitive gas emissions may potentially suffer adverse health effects from chronic exposure to this agent. Passive dosimeters can be worn in the breathing zone of potentially affected personnel during exposure periods, then later analyzed to quantify nitrous oxide exposure. This study sought to establish the accuracy of several commercially available passive monitoring devices for nitrous oxide by comparison against an infra-red spectrophotometric reference method.

Insulinlike Growth Factor 1- And 2-Augmented Collagen Gel Repair Of Facial Osseous Defects, James S. Toung, Roy C. Ogle, Raymond F. Morgan, William H. Lindsey

School of Medical Diagnostics & Translational Sciences Publications

BACKGROUND: Defects of the facial bone structure are common problems for the facial plastic surgeon. Native type 1 collagen gels (T1CGs) have been shown to mediate repair of facial critical-size defects in rat models.

OBJECTIVE: To evaluate the efficacy of T1CG augmented with insulinlike growth factor (IGF) 1, IGF-2, and a combination of IGF-1 and IGF-2 on the repair of facial critical-size defects in a rodent model.

METHODS: Twenty-four retired male breeder Sprague-Dawley rats were divided into 4 groups of 6 animals. Facial critical-size defects were created by removing the nasalis bones with a bone-cutting drill. Defects were treated with …

Induction Of Integral Membrane Pam Expression In Att-20 Cells Alters The Storage And Trafficking Of Pomc And Pc1, Giuseppe D. Ciccotosto, Martin R. Schiller, Betty A. Eipper, Richard E. Mains

Life Sciences Faculty Research

Peptidylglycine alpha-amidating monooxygenase (PAM) is an essential enzyme that catalyzes the COOH-terminal amidation of many neuroendocrine peptides. The bifunctional PAM protein contains an NH2-terminal monooxygenase (PHM) domain followed by a lyase (PAL) domain and a transmembrane domain. The cytosolic tail of PAM interacts with proteins that can affect cytoskeletal organization. A reverse tetracycline-regulated inducible expression system was used to construct an AtT-20 corticotrope cell line capable of inducible PAM-1 expression. Upon induction, cells displayed a time- and dose-dependent increase in enzyme activity, PAM mRNA, and protein. Induction of increased PAM-1 expression produced graded changes in PAM-1 metabolism. Increased expression of …

Study Of The Molecular Basis Of Diabetic Retinopathy, Heather Christine Glasscock-Hatcher

MUSC Theses and Dissertations

Abnormalities of the retinal vasculature contribute significantly to the development of diabetic retinopathy. In this study we have focused on two mechanisms which may be responsible-for the development and progression of this diabetic complication. Chronic hyperglycemia is associated with many of the vascular complications of diabetes mellitus; therefore, we examined mechanisms by which high-dose glucose damages retinal capillary endothelial cells. Our cell count results showed that high-dose glucose induces cell death in primary cultured human retinal capillary endothelial cells. Flow cytometry, in situ cell death detection, and transmission electron microscopy revealed that the cell death occurred via the apoptosis pathway …

Chapter 4: Normal And Anomalous Coronary Arteries In Humans. Part 1: Historical Background, Paolo Angelini, Salvado Villason, Albert V. Chan, Jr., Jose' G. Diez

Coronary Artery Anomalies

No abstract provided.

The Association Of Fluid Intake With Total Natural-Cause Mortality, Coronary Heart Disease And Fatal Stroke, Jacqueline Chung Lun Chan

Loma Linda University Electronic Theses, Dissertations & Projects

Hypohydration may be more widespread than realized since it does not always arouse a sense of thirst. When thirst is felt, it is often ignored, or satisfied before euhydration is achieved. There is growing evidence indicating that hypohydration contributes to acute and chronic illnesses, and to rheological factors associated with coronary heart disease (CHD) and stroke. Therefore the relationship of fluid intake with risk of total natural-cause mortality, CHD and stroke death was examined using data on 34,192 white, non-Hispanic participants in the Adventist Health Study.

Among the 27,342 reporting no CHD, stroke or diabetes at baseline, during six years …

Native Outreach: A Report To American Indian, Alaska Native And Native Hawaiian Communities, C.S. Glover, F.S. Hodge

Native Health Database Full Text

The National Cancer Institute (NCI) is presenting a monograph written specifically for community leaders, health professionals and lay health workers serving Native American communities. Native Americans have higher mortality rates than the general U.S. population for several major cancer sites. This publication focuses on the need to reduce those rates through effective interventions at the grassroots level.

The Efficacy Of A Developmental Follow-Up Program For High-Risk Infants, Laurie M. De Jong

Graduate Student Dissertations, Theses, Capstones, and Portfolios

Objective: To assess the efficacy of a developmental follow-up program provided at a local community hospital. Efficacy was measured by the number of children referred to early intervention services and the cost effectiveness of the program. Design: This is a retrospective study of 174 children evaluated during 272 visits in the Follow-up Clinic from the inception of the program on November 22, 1994 through December 31, 1998. The data collected included referral rates and demographic information on all children. Financial information was also obtained on 74 children seen during a one year period to assess the financial implications of the …

Needle Localization For Breast Biopsy: The Patient's Experience, Marian De Walt Morgan

Psychology Theses & Dissertations

The needle localization procedure for breast biopsy (NLP) can be painful and distressing for some women. This study was carried out to learn what factors might increase or decrease NLP pain and distress, and to gain insight into possible interventions to make the procedure more comfortable for all patients. One hundred and thirty-eight women were surveyed following breast needle localization at two central Virginia hospitals. The influence of eight variables (lidocaine, self-regulation, anxiety, worry about breast cancer, breast tenderness, finding mammography painful, difficulty with surgery, and distress of blood drawing) on four outcome variables (pain incidence, pain intensity, pain unpleasantness, …

Investigation Of The Contractile And Regulatory Proteins In Malignant Hyperthermia And Normal Skeletal Muscle, Louise C. Norbury

Theses : Honours

Malignant hyperthermia/hyperpyrexia (MH) is a serious human skeletal muscle disorder that manifests itself during general anaesthesia. Currently, the most reliable method of diagnosing this disease is the in-vitro contracture test (IVCT). This procedure is both highly invasive for the patient and expensive to perform. The skinned muscle fibre technique is less invasive, and could possibly be used as an alternative diagnostic test for MH. In this study, skeletal muscle samples were obtained from B adults (age 23-54 years) undergoing elective surgery at Royal Perth Hospital. Six patients were diagnosed as normal (controls) and two equivocal for MH (MHE). Using the …

Transient Expression Of A Translation Initiation Factor Is Conservatively Associated With Embryonic Gene Activation In Murine And Bovine Embryos., P A De Sousa, A J Watson, R M Schultz

Obstetrics & Gynaecology Publications

In the present study the abundance of mRNAs for eukaryotic translation initiation factors eIF-1A (formerly known as eIF-4C), -2alpha, -4A, -4E, and -5 was examined in in vivo-derived mouse embryos throughout preimplantation development using a semiquantitative reverse transcription-polymerase chain reaction assay. Although the mRNA profile for each gene is unique, only mRNA for eIF-1A transiently increases during embryonic gene activation (EGA) at the 2-cell stage, and this was confirmed by an independent hybridization-based assay. In in vitro-developed bovine embryos, mRNA for eIF-1A was transiently detected at the 8-cell stage, when the major activation of the genome occurs in this species. …

Mouse Embryo Development In The Presence Of Capsaicin, Carlos Santiago Villar-Gosalvez

Theses and Dissertations in Biomedical Sciences

Capsaicin is the pungent agent found in hot peppers of the Capsicum genus. It is a potent neurotoxin that stimulates the degranulation and degeneration of C-afferent neurons. Capsaicin is widely used as a food condiment and medicine. Human exposure of capsaicin can exceed levels shown to be neurotoxic in laboratory animals. Additionally, capsaicin can cross the blood/placenta barrier and affect an embryo in utero. In order to assay the potential for toxicity to human embryos, mouse embryos were exposed to capsaicin and the effect of the capsaicin on embryo development was measured. Embryos were co-cultured in Krebs medium with 1% …

Department Of Radiology-Annual Report-July 1, 1997 To June 30, 1998, David C. Levin

Department of Radiology Administrative Documents and Reports

Department of Radiology Annual Executive Summary Report, July 1, 1997 to June 30, 1998. Thomas Jefferson University Hospital, Philadelphia, Pennsylvania, United States. 103 pages.

The Relation Of Weight Loss And Improved Fitness To Survival In Healthy Males, Joanna Lynn Bokovoy

Loma Linda University Electronic Theses, Dissertations & Projects

Obesity is a critical public health problem because of its prevalence, medical and psychosocial effects, and resistance to intervention. Weight loss in overweight individuals has been encouraged by the United States Public Health Service and by the medical community as a means for improving health. However, recent studies have suggested either positive or negative effects of weight loss on survival. It is possible that physical fitness may have a confounding effect, or be an effect modifier on survival in weight loss studies.

This study used Cox Regression analysis to address the separate and combined association of weight loss and improved …

Cholera In Children In Karachi From 1990 Through 1995: A Study Of Cases Admitted To A Tertiary Care Hospital, S Q. Nizami, B J. Farooqui

Department of Paediatrics and Child Health

Although cholera is an endemic disease in Bangladesh, India and other countries, it was never a significant cause of gastroenteritis in Pakistan before 1988. Since then, cases of cholera are identified each year, both in adults and children in Pakistan. In order to see the contribution of Vibrio cholerae as a cause of gastroenteritis in children, we reviewed the cases of cholera admitted in the pediatric ward of the Aga Khan University Hospital, Karachi, Pakistan. Of 4346 children hospitalized with gastroenteritis during 1990 through 1995, 348 children (8%) were confirmed to have cholera. The youngest child with cholera was seven …

Nursing Home Ownership And Public Policy: An Historical Analysis, K. R. Kaffenberger

Graduate Doctoral Dissertations

In the early days of the United States, care of the disabled elderly outside the home meant the public almshouse. By the 1920s, private, nonprofit homes for the aged were prevalent. More recently, private, for-profit facilities have grown to dominate the field.

For-profit ownership has been controversial. Underlying the controversy is the concern that quality might be lowered in order to enhance profit.

This study asks why most nursing homes are privately owned and why most privately owned nursing homes are operated for profit. It does so with reference to The Nonprofit Economy, in which Burton Weisbrod describes a …

Digest Of The Philadelphia College Of Osteopathic Medicine (Spring 1998), Philadelphia College Of Osteopathic Medicine

Digest

The Spring 1998 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Opening Remarks
• PCOM Updates
• Our Centennial
• Planting the Seeds for Better Physician Communication
• Library Steps into 21st Century
• Convention Highlights
• Uncle Manny Leaves a Legacy
• Mission Milestones
• Profiles
• Lights, Camera, Action
• Class Notes
• Just Published
• In Memoriam
• Coming Events

The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar

Theses and Dissertations in Biomedical Sciences

Using the hemizona assay (HZA), a in vitro sperm-egg binding assay, we show that specific glycoconjugates known to inhibit immune cell interactions mediated by the selectins, potently block human sperm-egg binding. The selectin ligand sialyl Lewis^x inhibits sperm binding in the HZA by 60% at a concentration of 1 mg/ml. Our data indicates that glycodelin-A, a endometrial glycoprotein known to block sperm-egg binding in the HZA at low concentrations expresses unusual fucosylated lacdiNAc type glycans. The fucosylated lacdiNAc type sugars have been previously shown to be 15-20 fold more potent ligands of E-selectin. Glycodelin-S a seminal plasma glycoform of …

Oncolog, Volume 43, Number 02, February 1998, Beth W. Allen, Alison Ruffin, Rebecca D. Pentz Ph.D.

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Genetic findings prompt cancer survivor to elect surgery
• Protocols: Radiotherapy Protocols Offer Treatment for Range of Sites and Types of Cancer
• Radiation oncology: targeting effective treatment
• House Call: Cancer: Is it in the Genes or in the Stars?
• DiaLog: Genetic Tests Demand New Answers by Rebecca D. Pentz, PhD, Clinical Ethicist

Oncolog, Volume 43, Number 01, January 1998, Sunita Patterson, Stephanie Deming, Therese Bevers Md

OncoLog MD Anderson's Report to Physicians (All issues) Archives

• Taking Mammography on the Road
• Reassurance and Education Foster Rehabilitation After Colorectal Surgery
• Protocols: Protocols focus on chemoprevention, improving preoperative radiotherapy, and treating advance-stage colorectal cancer
• Sphincter-Preserving Surgery Improves Outcome for Patients with Rectal Cancer
• House Call: Stopping Cancer Before It Occurs: Spell P-r-e-v-e-n-t-i-o-n
• Dialog: Reaching Out and In by Therese Bevers, MD, Cancer Prevention Center Director
• Protocols: Protocols focus on chemoprevention, improving preoperative radiotherapy, and treating advance-stage colorectal cancer

Digest Of The Philadelphia College Of Osteopathic Medicine (Winter 1998), Philadelphia College Of Osteopathic Medicine

Digest

The Winter 1998 issue of the Digest of the Philadelphia College of Osteopathic Medicine includes the following articles:

• Opening Remarks
• PCOM Updates
• Our Centennial
• Founders Day '98
• A Doctor's Helping Hand
• PCOM Honors Domenic A. Debias, Phd
• The Physician Assistant
• Mission Milestones
• Profiles
• Convention Highlights
• Class Notes
• Just Published
• In Memoriam
• Coming Events

Rapport Du Seminaire National [Au Mali] Pour La Definition D'Une Strategie D'Eradication De L'Excision, Population Council

Reproductive Health

This is a report on a workshop for the eradication of female genital mutilation (FGM) held in Bamako, Mali. The workshop presented the results of a literature review on FGM and the actions taken to counter the practice in Mali; provided updates on the activities of six national NGOs working on issues surrounding FGM; and developed a Plan of Action for activities aimed at reducing the practice. The workshop brought together 85 participants, including policy- and decisionmakers from the government, NGOs, and religious groups. The workshop led to the finalization of a national Plan of Action to the Year 2000 …

Ms211: Plans For Nazarbayev Visit To Houston And Tmc, Armin Weinberg

Works on Radiation Effects: 1990-2020

A list of things that Armin created in preparation for the visit by the President of Kazakhstan to Texas, Houston and the Medical Center. Very insightful with names and activities. See more at Armin Weinberg, PhD Papers and its finding aid.

Permeable, Non-Irritating Prodrugs Of Nonsteroidal And Steroidal Agents, Paul Ashton, Thomas J. Smith, Peter G. Glavinos, John D. Conklin, Peter A. Crooks, Robert M. Riggs, Tadeusz Cynkowski, Grazyna Cynkowska

Ophthalmology and Visual Science Faculty Patents

Prodrugs containing an active drug molecule linked to a polyethylene glycol group, and a method of use thereof are described. Exemplary soluble ester prodrugs contain naproxen, triamcinolone acetonide, gancyclovir, taxol, cyclosporin, dideoxyinosine, trihydroxy steroids, and flurbiprofen molecules linked to polyethylene glycol (PEG) groups. Pharmaceutical compositions containing these prodrugs, and a method of using these esters for treating disease states or symptoms are also described.