Digital Commons Network^™

Macrophage Sensing Of Single- Walled Carbon Nanotubes Via Toll- Like Receptors, Sourav P. Mukherjee, Olesja Bondarenko, Pekka Kohonen, Fernando T. Andon, Tana Brzicova, Isabel Gessner, Sanjay Mathur, Massimo Bottini, Paolo Calligari, Lorenzo Stella, Elena Kisin, Anna Shvedova, Reija Autio, Heli Salminen-Mankonen, Ritta Lahesmaa, Bengt Fadeel

Faculty & Staff Scholarship

Carbon-based nanomaterials including carbon nanotubes (CNTs) have been shown to trigger

inflammation. However, how these materials are ‘sensed’ by immune cells is not known. Here we compared the effects of two carbon-based nanomaterials, single-walled CNTs (SWCNTs) and graphene oxide (GO), on primary human monocyte-derived macrophages. Genome-wide transcriptomics assessment was performed at sub-cytotoxic doses. Pathway analysis of the microarray data revealed pronounced effects on chemokine-encoding genes in macrophages exposed to SWCNTs, but not in response to GO, and these results were validated by multiplex array-based cytokine and chemokine profiling. Conditioned medium from SWCNT-exposed cells acted as a chemoattractant for dendritic cells. …

Absolute Lymphocyte And Neutrophil Counts In Neonatal Ischemic Brain Injury, Jessica M. Povroznik, Elizabeth B. Engler-Chiurazzi, Tania Nanavati, Paola Pergami

Faculty & Staff Scholarship

Objectives: This study aimed to identify differences in absolute neutrophils, lymphocytes, and neutrophil-to-lymphocyte ratio between neonates with two forms of ischemic brain injury, hypoxic-ischemic encephalopathy, and acute ischemic stroke, compared to controls. We also aimed to determine whether this neutrophil/lymphocyte response pattern is associated with disease severity or is a consequence of the effects of total-body cooling, an approved treatment for moderate-to-severe hypoxic-ischemic encephalopathy. Methods: A retrospective chart review of 101 neonates with hypoxic-ischemic encephalopathy + total-body cooling (n = 26), hypoxic-ischemic encephalopathy (n=12), acute ischemic stroke (n=15), and transient tachypnea of the newborn (n=48) was conducted; transient tachypnea of …

Perfluoroalkyl Substances And Kidney Function In Chronic Kidney Disease, Anemia, And Diabetes, Baqiyyah N. Conway, Ashley N. Badders, Tina Costacou, John M. Arthur, Kim E. Innes

Faculty & Staff Scholarship

Background: Anemia often complicates chronic kidney disease (CKD), leading to insufficient tissue oxygenation and hypoxic injury, the factor thought to underlie progression from CKD to renal failure. Perfluorocarbons are potent oxygen transporters used in organ preservation and synthetic blood development. Data are scarce on their relationship with kidney function, especially in diabetes where anemia and hypoxia are more prevalent. We investigated the relationship of perfluoroalkyl acids (PFAS) with kidney function and variation by diabetes and anemia status.

Methods: Data on 53,650 adults (5,210 with diabetes) were obtained from the C8 Health Project. CKD was defined as an estimated glomerular filtration …

Optimal Scene Time To Achieve Favorable Outcomes In Out-Of-Hospital Cardiac Arrest: How Long Is Too Long?, Glenn Goodwin, Dyana Picache, Brian J. Louie, Nicholas Gaeto, Tarik Zeid, Paxton P. Aung, Sonu Sahni

Touro College of Osteopathic Medicine (New York) Publications and Research

Despite advances in resuscitation science and public health, out-of-hospital cardiac arrest (OOHCA) cases have an average survival rate of only 12% nationwide, compared to 24.8% of cases occurring in hospital. Many factors, including resuscitation interventions, contribute to positive patient outcomes and have, therefore, been studied in attempts to optimize emergency medical services (EMS) protocols to achieve higher rates of return of spontaneous circulation (ROSC) in the field. However, no consensus has been met regarding the appropriate amount of time for EMS to spend on scene.

Evaluation Of Concussion Incidence And Modulating Factors In The 2013-2017 Australian Football League, Ryan Adams, George K. Lau, Jennifer B. Dai, Adam Y. Li, Kevin P. Cheung, Syed Haider, Alex Gometz

Touro College of Osteopathic Medicine (New York) Publications and Research

The increasing awareness and popularization of concussions in the research realm over the last few years have begun to shed more light on the detrimental effects associated with repetitive head trauma. While the majority of the current literature focuses on the National Football League (NFL) and National Hockey League (NHL), several other high-impact sports have been implementing concussion management protocols to protect their players. The Australian Football League (AFL) is a prime example of a major contact sport that has undertaken recent changes to its concussion assessment and management modalities. We recognize the benefit of reporting potential changes in concussion …

Total Sulfane Sulfur Bioavailability Reflects Ethnic And Gender Disparities In Cardiovascular Disease, Saurabh Rajpal, Pavan Katikaneni, Matthew Deshotels, Sibile Pardue, John Glawe, Xinggui Shen, Nuri Akkus, Kalgi Modi, Ruchi Bhandari, Paari Dominic, Pratap Reddy, Gopi K. Kolluru, Christopher G. Kevil

Faculty & Staff Scholarship

Hydrogen sulfide (H2S) has emerged as an important physiological and pathophysiological signaling molecule in the cardiovascular system influencing vascular tone, cytoprotective responses, redox reactions, vascular adap- tation, and mitochondrial respiration. However, bioavailable levels of H2S in its various biochemical metabolite forms during clinical cardiovascular disease remain poorly understood. We performed a case-controlled study to quantify and compare the bioavailability of various biochemical forms of H2S in patients with and without cardiovascular disease (CVD). In our study, we used the reverse-phase high performance liquid chromatography monobromobimane assay to analytically measure bioavailable pools of H2S. Single nucleotide polymorphisms (SNPs) were also identified …

Anti-Tau Conformational Scfv Mc1 Antibody Efficiently Reduces Pathological Tau Species In Adult Jnpl3 Mice., F. Vitale, L. Giliberto, S. Ruiz, K. Steslow, P. Marambaud, C. D'Abramo

Journal Articles

Tau, the main component of the neurofibrillary tangles (NFTs), is an attractive target for immunotherapy in Alzheimer's disease (AD) and other tauopathies. MC1/Alz50 are currently the only antibodies targeting a disease-specific conformational modification of tau. Passive immunization experiments using intra-peritoneal injections have previously shown that MC1 is effective at reducing tau pathology in the forebrain of tau transgenic JNPL3 mice. In order to reach a long-term and sustained brain delivery, and avoid multiple injection protocols, we tested the efficacy of the single-chain variable fragment of MC1 (scFv-MC1) to reduce tau pathology in the same animal model, with focus on brain …

Identification Of Ethyl Pyruvate As A Nlrp3 Inflammasome Inhibitor That Preserves Mitochondrial Integrity, S. Li, F. Liang, K. Kwan, Y. Tang, X. Wang, H. Yang, S. S. Chavan, H. Wang, K. J. Tracey, +3 Additional Authors

Journal Articles

No abstract provided.

Induction And Quantification Of Excitability Changes In Human Cortical Networks, C. J. Keller, Y. Huang, J. L. Herrero, M. E. Fini, V. Du, F. A. Lado, C. J. Honey, A. D. Mehta

Journal Articles

No abstract provided.

Hepatitis C Infection Patterns At A Tertiary Care Center In New York: A Cross-Sectional Study., A. Mahgoub, T. El Imad, H. Al Moussawi, D. Daneshvar, F. G. Haddad, J. Saabiye, G. Abdelsayed, S. Andrawes, L. Deeb

Journal Articles

Introduction In the United States, 2.7 to 3.9 million patients are infected with the hepatitis C virus (HCV) with 3,500 new cases reported yearly. According to the Centers for Disease Control and Prevention, HCV was the underlying or contributing cause of death of 19,659 patients in 2014. These facts underscore the need for a better understanding of the scope of this disease. Our epidemiologic study aimed at analyzing the pattern of occurrence of HCV infection at Staten Island University Hospital (SIUH) by evaluating the characteristics of newly infected patients with hepatitis C in 2014. The identified features served to better …

Indwelling Pleural Catheters: Complications And Management Strategies, M. Chalhoub, A. Saqib, M. Castellano

Journal Articles

©Journal of Thoracic Disease. Indwelling pleural catheters (IPCs) are increasingly being used for patients with recurrent malignant pleural effusions. They are simple to place and can be done on an outpatient basis under local anesthesia. IPCs uniformly relieve dyspnea and improve quality of life of patients with malignant pleural effusions. In some patients with recurrent non-malignant pleural effusions, IPCs proved to be effective as well. With increasing use of IPCs, physicians and patients are faced with complications related to the presence of an indwelling catheter for extended periods of time. The purpose of this review is to describe the various …

Apol1 Risk Variants Cause Podocytes Injury Through Enhancing Endoplasmic Reticulum Stress, H. Wen, V. Kumar, X. Lan, S. S. Shoshtari, J. M. Eng, X. Zhou, F. Wang, H. Wang, A. Malhotra, P. C. Singhal, +4 Additional Authors

Journal Articles

c 2018 The Author(s). Two coding sequence variants (G1 and G2) of Apolipoprotein L1 (APOL1) gene have been implicated as a higher risk factor for chronic kidney diseases (CKD) in African Americans when compared with European Americans. Previous studies have suggested that the APOL1 G1 and G2 variant proteins are more toxic to kidney cells than the wild-type APOL1 G0, but the underlying mechanisms are poorly understood. To determine whether endoplasmic reticulum (ER) stress contributes to podocyte toxicity, we generated human podocytes (HPs) that stably overexpressed APOL1 G0, G1, or G2 (Vec/HPs, G0/HPs, G1/HPs, and G2/HPs). Propidium iodide staining showed …

Epigallocatechin-3-Gallate Confers Protection Against Corticosterone-Induced Neuron Injuries Via Restoring Extracellular Signal-Regulated Kinase 1/2 And Phosphatidylinositol-3 Kinase/Protein Kinase B Signaling Pathways, X. Zhao, R. Li, H. Jin, H. Jin, Y. Wang, W. Zhang, H. Wang, W. Chen

Journal Articles

No abstract provided.

Transrenal Ureteral Embolization Utilizing Amplatzer Vascular Plugs And N-Butyl Cyanoacrylate Glue., M. X. Jin, A. D. Mohabir, D. M. Caplin, I. Lobko, D. N. Siegel

Journal Articles

This retrospective study presents three consecutive patients who underwent bilateral ureteral occlusion using the Amplatzer vascular plugs and N-butyl cyanoacrylate glue sandwich method. The patients were 63- and 65-year-old males and a 79-year-old female. Indications for the procedure included severe cystitis and complex vesicular fistulas unresponsive to urinary diversion. All three patients had immediate resolution of urinary leakage, resulting in symptom relief throughout the follow-up period. There were no procedure-related complications or side effects.

Management Of Acute Pancreatitis In The Pediatric Population: A Clinical Report From The North American Society For Pediatric Gastroenterology, Hepatology And Nutrition Pancreas Committee, M. Abu-El-Haija, S. Kumar, J. A. Quiros, K. Balakrishnan, B. Barth, S. Bitton, J. F. Eisses, E. J. Foglio, V. Fox, V. D. Morinville, +14 Additional Authors

Journal Articles

No abstract provided.

Computational Linguistic Analysis Applied To A Semantic Fluency Task To Measure Derailment And Tangentiality In Schizophrenia, L. Pauselli, B. Halpern, S. D. Cleary, B. Ku, M. A. Covington, M. T. Compton

Journal Articles

No abstract provided.

Healthy Adolescent Performance With Standardized Scoring Tables For The Matrics Consensus Cognitive Battery: A Multisite Study., R. E. Smelror, K. N. Jørgensen, V. Lonning, I. Kelleher, M. Cannon, P. Derosse, A. K. Malhotra, K. H. Karlsgodt, O. A. Andreassen, I. Agartz, +6 Additional Authors

Journal Articles

The aim of this study was to develop standardized scores and scoring tables for test performance in healthy adolescents for the Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery (MCCB) for each year from 11 to 19 years of age, by sex, with T scores and percentile ranks.A total of 502 healthy participants (aged 11-19 years) from 7 cohorts from Ireland, Norway, Sweden, and United States, were included in this multisite study. Regression-predicted means for the MCCB tests, except the social cognition subtest, were calculated using the MCCB test scores as outcome variables and age, …

The Role Of Genetics In Advancing Precision Medicine For Alzheimer's Disease-A Narrative Review., Y. Freudenberg-Hua, W. Li, P. Davies

Journal Articles

Alzheimer's disease (AD) is the most common type of dementia, which has a substantial genetic component. AD affects predominantly older people. Accordingly, the prevalence of dementia has been rising as the population ages. To date, there are no effective interventions that can cure or halt the progression of AD. The only available treatments are the management of certain symptoms and consequences of dementia. The current state-of-the-art medical care for AD comprises three simple principles: prevent the preventable, achieve early diagnosis, and manage the manageable symptoms. This review provides a summary of the current state of knowledge of risk factors for …

Cold-Inducible Rna-Binding Protein Activates Splenic T Cells During Sepsis In A Tlr4-Dependent Manner, A. C. Bolognese, A. Sharma, W. Yang, J. Nicastro, G. F. Coppa, P. Wang

Journal Articles

No abstract provided.

Cirp Increases Icam-1(+) Phenotype Of Neutrophils Exhibiting Elevated Inos And Nets In Sepsis, Y. Ode, M. Aziz, P. Wang

Journal Articles

No abstract provided.

Activation Of Invariant Natural Killer T Cells Redirects The Inflammatory Response In Neonatal Sepsis, A. C. Bolognese, W. Yang, L. W. Hansen, A. Sharma, J. M. Nicastro, G. F. Coppa, P. Wang

Journal Articles

No abstract provided.

Neonatal Intestinal Dysbiosis In Necrotizing Enterocolitis, N. Denning, J. M. Prince

Journal Articles

No abstract provided.

B-1a Cells Protect Mice From Sepsis-Induced Acute Lung Injury, M. Aziz, Y. Ode, M. Zhou, M. Ochani, N. E. Holodick, T. L. Rothstein, P. Wang

Journal Articles

No abstract provided.

The Protective Role Of Human Ghrelin In Sepsis: Restoration Of Cd4 T Cell Proliferation, M. Zhou, M. Aziz, M. Ochani, W. Yang, A. Sharma, P. Wang

Journal Articles

No abstract provided.

Elevated Urine Levels Of Macrophage Migration Inhibitory Factor In Inflammatory Bladder Conditions: A Potential Biomarker For A Subgroup Of Interstitial Cystitis/Bladder Pain Syndrome Patients, P. L. Vera, D. M. Preston, R. M. Moldwin, D. R. Erickson, B. Mowlazadeh, F. Ma, D. E. Kouzoukas, K. L. Meyer-Siegler, M. Fall

Journal Articles

No abstract provided.

Assessment Of A Mouse Xenograft Model Of Primary Colorectal Cancer With Special Reference To Perfluorooctane Sulfonate, Jeffrey H. Wimsatt, Caitlin Montgomery, Laurel S. Thomas, Charity Savard, Rachel Tallman, Kim Innes, Nezar Jrebi

Faculty & Staff Scholarship

Colorectal cancer ranks third among the most commonly diagnosed cancers in the United States. Current therapies have a range of side effects, and the development of a reliable animal model to speed the discovery of safe effective preventative therapies would be of great value. A cross-sectional study in a large Appalachian population recently showed an association between low circulating levels of perfluorooctane sulfonate (PFOS) and a reduced prevalence of colorectal cancer. A study using APCmin(C57BL/6J-ApcMin/J) mice prone to familial adenomatous polyposis found PFOS was protective when exposure occurred during tumor development. To test the possible benefit of PFOS on spontaneous …

Metabolic And Transcriptional Modules Independently Diversify Plasma Cell Lifespan And Function, Wing Y. Lam, Arijita Jash, Cong-Hui Yao, Lucas D'Souza, Rachel Wong, Ryan M. Nunley, Gordon P. Meares, Gary J. Patti, Deepta Bhattacharya

Faculty & Staff Scholarship

Plasma cell survival and the consequent duration of immunity vary widely with infection or vaccination. Using fluorescent glucose analog uptake, we defined multiple developmentally independent mouse plasma cell populations with varying life- spans. Long-lived plasma cells imported more fluo- rescent glucose analog, expressed higher surface levels of the amino acid transporter CD98, and had more autophagosome mass than did short-lived cells. Low amino acid concentrations triggered re- ductions in both antibody secretion and mitochon- drial respiration, especially by short-lived plasma cells. To explain these observations, we found that glutamine was used for both mitochondrial respira- tion and anaplerotic reactions, yielding …

Hybrid Capture–Based Genomic Profiling Of Circulating Tumor Dna From Patients With Advanced Cancers Of The Gastrointestinal Tract Or Anus, A. B. Schrock, D. Pavlick, S. J. Klempner, J. H. Chung, B. Forcier, A. Welsh, L. Young, B. Leyland-Jones, C. Devoe, V. A. Miller, +9 Additional Authors

Journal Articles

© 2018 American Association for Cancer Research. Purpose: Genomic profiling of tumor biopsies from advanced gastrointestinal and anal cancers is increasingly used to inform treatment. In some cases, tissue biopsy can be prohibitive, and we sought to investigate whether analysis of blood-derived circulating tumor DNA (ctDNA) may provide a minimally invasive alternative. Experimental Design: Hybrid capture–based genomic profiling of 62 genes was performed on blood-based ctDNA from 417 patients with gastrointestinal carcinomas to assess the presence of genomic alterations (GA) and compare with matched tissue samples. Results: Evidence of ctDNA was detected in 344 of 417 samples (82%), and of …

Considerations In The Diagnosis Of Chronic Granulomatous Disease, J. E. Yu, A. E. Azar, H. J. Chong, A. M. Jongco, B. T. Prince

Journal Articles

© The Author(s) 2018. Chronic granulomatous disease (CGD) is a rare primary immunodeficiency that is caused by defects in the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. The disease presents in most patients initially with infection, especially of the lymph nodes, lung, liver, bone, and skin. Patients with CGD are susceptible to a narrow spectrum of pathogens, and Staphylococcus aureus, Burkholderia cepacia complex, Serratia marcescens, Nocardia species, and Aspergillus species are the most common organisms implicated in North America. Granuloma formation, most frequently in the gastrointestinal and genitourinary systems, is a common complication of CGD and can be seen even …

Examining The Impact Of A Multimedia Intervention On Treatment Decision-Making Among Newly Diagnosed Prostate Cancer Patients: Results From A Nationwide Rct., M. A. Diefenbach, C. Benedict, S. M. Miller, A. L. Stanton, M. E. Ropka, K. Wen, N. E. Mohamed, S. J. Hall

Journal Articles

Men diagnosed with early stage prostate cancer face multiple treatment options, each with distinctive side effects that have significant implications for post-treatment quality of life. Healing Choices for Men with Prostate Cancer is a multimedia educational and decision aid program. This nation-wide randomized controlled trial evaluated the impact of Healing Choices on reducing decisional conflict and distress. Eligible prostate cancer patients who called the National Cancer Institute's Cancer Information Service (CIS) were invited to participate. After a baseline interview, participants were randomized to usual personalized consultation with a CIS specialist (comparison condition) or CIS personalized consultation plus the Healing Choices …