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The Growth Of Simian Virus 40 (Sv40) Host Range/Adenovirus Helper Function Mutants In An African Green Monkey Cell Line That Constitutively Expresses The Sv40 Agnoprotein., Terryl P. Stacy, Michele Chamberlain, Susan Carswell, Charles N. Cole

Dartmouth Scholarship

The simian virus 40 T-antigen carboxy-terminal mutants, dlA2459 and dlA2475, are cell line and temperature dependent for growth and plaque formation in monkey kidney cells. Although these mutants did form plaques on BSC-1 cells at 37 degrees C, they were about fivefold less efficient for plaque formation than wild-type simian virus 40. These mutants did not grow in CV-1 cells and did not synthesize agnoprotein in those cells. CV-1 cells which constitutively express the agnoprotein were permissive for mutant plaque formation. However, late mRNAs, virion proteins, and progeny virion yields did not accumulate to wild-type levels during mutant infection of …

Relationship Of Dietary Aluminum, Phosphorus, And Calcium To Phosphorus And Calcium Metabolism And Growth Performance Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

Dietary treatments providing three levels of added Al (0, .196, or .392%) as aluminum sulfate and of available phosphorus (P_av) (.45, .68, or .78%) in a factorial arrangement were administered to day-old chicks in Experiment 1. Plasma inorganic phosphorus (P_i) was significantly (P < .05) elevated by increasing P_av and was decreased by Al. Body weight gain, feed intake, and the gain:feed ratio at Day 21 were significantly decreased by increased concentrations of Al, but were unaffected by the P_av concentrations. Decreases of 39 and 73% in weight gain and of 34 and 66% in feed intake resulted from feeding …

Mechanism Of Escape Of Endogenous Murine Leukemia Virus Emv-14 From Recognition By Anti-Akr/Gross Virus Cytolytic T Lymphocytes., Hillary D. White, Michael D. Robbins, William R. Green

Dartmouth Scholarship

It was previously shown that spleen cells from endogenous ecotropic murine leukemia virus emv-14+ AKXL-5 mice fail to stimulate an anti-AKR/Gross virus cytolytic T-lymphocyte (CTL) response in a mixed lymphocyte culture with primed C57BL/6 responder spleen cells, whereas spleen cells from AKXL strains carrying the very similar emv-11 provirus do stimulate a response (Green and Graziano, Immunogenetics 23:106-110, 1986). We wished to determine whether the lack of response with AKXL-5 spleen cells was at the level of recognition between effector cell and target cell and whether the relevant mutation was within the emv-14 provirus. It is shown here that EMV-negative …

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

Progesterone And 17 Α-Hydroxyprogesterone: Novel Stimulators Of Calcium Influx In Human Sperm, Peter F. Blackmore, Stephen J. Beebe, Douglas R. Danforth, Nancy Alexander

Bioelectrics Publications

Progesterone and 17 alpha-hydroxyprogesterone (but not other steroids such as testosterone, corticosterone, beta-estradiol, estrone, dehydroepiandrosterone, 20 alpha-hydroxypregnen-3-one, androstenedione, and pregnenolone) were shown to cause an immediate increase, in free cytosolic calcium ([Ca2+]i) in both capacitated and noncapacitated human sperm, using the fluorescent indicator fura 2. Significant increases in [Ca2+]i were observed with 10 ng/ml progesterone, while maximum effects were seen with 1 microgram/ml progesterone. Two other steroids 11 beta-hydroxyprogesterone and 5 alpha-pregnane-3,20-dione exhibited significant activity to increase [Ca2+]i. This increase in [Ca2+]i elicited by progesterone was entirely due to Ca2+ influx from the extracellular medium since the increase in [Ca2+]i …

Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole

Dartmouth Scholarship

Simian virus 40 (SV40) deletion mutants dlA2459 and dlA2475 express T antigens that lack the normal carboxy terminus. These mutants are called host range/helper function (hr/hf) mutants because they form plaques at 37 degrees C on BSC-1 and Vero monkey kidney cell lines but not on CV-1p monkey kidney cells. Wild-type SV40 can provide a helper function to permit growth of human adenoviruses in monkey kidney cells; the hr/hf mutants cannot. Progeny yields of hr/hf mutants are also cold sensitive in all cell lines tested. Patterns of viral macromolecular synthesis in three cell lines (Vero, BSC-1, and CV-1) at three …

Significance Of Premature Stop Codons In Env Of Simian Immunodeficiency Virus, Toshiaki Kodama, Dawn P. Wooley, Yathirajulu M. Naidu, Harry W. Kestler Iii, Muthiah D. Daniel, Yen Li, Ronald C. Desrosiers

Neuroscience, Cell Biology & Physiology Faculty Publications

The location of the translational termination codon for the transmembrane protein (TMP) varies in three infectious molecular clones of simian immunodeficiency virus from macaques (SIV_mac). The SIV_mac251 and SIV_mac142 infectious clones have premature stop signals that differ in location by one codon; transfection of these DNAs into human HUT-78 cells yielded virus with a truncated TMP (28 to 30 kilodaltons [kDa]). The SIV_mac239 infectious clone does not have a premature stop codon in its TMP-coding region. Transfection of HUT-78 cells with this clone initially yielded virus with a full-length TMP (41 kDa). …

Linker Insertion Mutants Of Simian Virus 40 Large T Antigen That Show Trans-Dominant Interference With Wild-Type Large T Antigen Map To Multiple Sites Within The T-Antigen Gene., Jiyue Y. Zhu, Charles N. Cole

Dartmouth Scholarship

Linker insertion mutants affecting the simian virus 40 (SV40) large tumor (T) antigen were constructed by inserting a 12-base-pair oligonucleotide linker into restriction endonuclease cleavage sites located within the early region of SV40. One mutant, with the insertion at amino acid 5, was viable in CV-1p and BSC-1 cells, indicating that sequences very close to the amino terminus of large T could be altered without affecting the lytic infection cycle of SV40. All other mutants affecting large T were not viable. In complementation assays between the linker insertion mutants and either a late-gene mutant, dlBC865, or a host range/helper function …

The Determination Of Tamoxifen Citrate In Human Blood Serum And Semen, Karimdad Tahmoresi

Chemistry & Biochemistry Theses & Dissertations

High performance liquid chromatography (HPLC)methodologies for the determination of tamoxifen and its metabolites 4-hydroxytamoxifen and n-desmethyltamoxifen, and using cis-tamoxifen as an internal standard have been developed.

Clinical analyses of tamoxifen and its metabolites in human serum and semen, were performed as follows: The analytes were extracted from serum and semen with hexane, the hexane evaporated, the dried extract then reconstituted with methanol, followed by reverse phase high performance liquid chromatography.

Between-run coefficients of variation (CV'S) for tamoxifen and n-desmethyltamoxifen for serum sample analyses, (n = 15) were, 4% and 13% at mean (SD) concentration of 140.2 (6.2) and 71.8 (9.8) …

Psychophysiological Assessment Of Panic Disorder, Kent A. Koehn

Masters Theses

This study compared physiological and psychological measures between 9 subjects meeting the DSM-III-R criteria for panic disorder (PD) and 9 control subjects to identify psychophysiological differences that might be relevant to the etiology and maintenance of panic attacks. The subjects were assessed through a number of self-report measures and across three laboratory sessions involving five experimental conditions: baseline, role play, relaxation, mental arithmetic, and cold pressor. We measured electrodermal (EDG) and electro-myographic activity (EMG), heart rate (HR), and blood volume pulse (BVP) in terms of absolute values, reactivity, and habituation. Both groups were also assessed in their ability to accurately …

Cessation Of Chronic Ethanol Intake Reverses Ultrastructural Alterations In Ileal M Cells, Robert Rosback Eversole

Masters Theses

Membranous (M) cells of the dome epithelium of ileal Peyer's patches have been shown to serve an antigen uptake function for mucosa associated lymphoid tissue. Hypergammaglobulinemia is a frequent accompaniment of chronic alcoholism in humans and the elevated antibody titers have been shown to be due in part to bacterial and dietary proteins. This study was conducted to demonstrate the morphological changes induced by chronic ethanol exposure in the ileal M cells in the rat.

Chronic ingestion of ethanol produces marked changes in the ultrastructure of M cells. All experimental animals showed distinct mitochondrial swelling with loss of matrix density, …

Maternal-Neonatal Serum Vitamin A Concentrations., Girish S. Shirali, D G. Oelberg, K P. Mehta

Manuscripts, Articles, Book Chapters and Other Papers

Prevention of neonatal vitamin A deficiency is related to the adequacy of maternal vitamin A stores. In this study we investigated maternal and cord serum vitamin A and retinol-binding protein (RBP) values in an Indian population including, for the first time, clinically vitamin A-deficient mothers. Twenty-eight maternal-neonatal pairs were selected from maternal cohorts of high socioeconomic status without clinical evidence of vitamin A deficiency (group I) and low socioeconomic status with conjunctival xerosis and Bitot's spots (group II). Maternal education, caloric and vitamin A intakes, weight, height, hemoglobin, and birth weight were significantly lower in group II. Serum vitamin A …

Evidence For Physiological Integration Between Shaded And Unshaded Short Shoots Of Thalassia Testudinum, David A. Tomasko, Clinton J. Dawes

Integrative Biology Faculty and Staff Publications

Physiological integration of Thalassia testudinum short shoots enables clones to function at a higher level of physiological organization than that of the short shoots themselves. Shaded short shoots connected to non-shaded short shoots had blade growth rates and proximate organic constituent levels equal to non-shaded controls. Shaded short shoots physically isolated from neighboring short shoots had blade growth rates and organic constituent levels different from both controls and shaded short shoots connected to non-shaded short shoots. Support of shaded short shoots appeared to be primarily from older short shoots connected to the shaded short shoots. The amelioration of localized light …

1989 Spring No. 19, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 19 Spring 1989 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Structure And Function Of Human Hemoglobin Covalently Labeled With Periodate-Oxidized Adenosine Triphosphate, Michael Kavanaugh, Max F. Perutz, Giulio Fermi, Daniel T. Shih, Richard T. Jones

Biomedical and Pharmaceutical Sciences Faculty Publications

Periodate-oxidized adenosine triphosphate (o-ATP), a ribose ring-opened dialdehyde derivative of ATP, reacts specifically with human deoxyhemoglobin to give a single major covalently modified product after reduction with sodium borohydride. This product, designated di-ATP Hb, was isolated using ion-exchange chromatography and shown to have incorporated two molecules of o-ATP/tetramer. Peptide mapping and x-ray crystallography at 2.8-A resolution indicate that a covalent adduct is formed between the ligand and residues Lys-82 EF6 of each beta chain in the organic phosphate-binding site of the molecule. di-ATP Hb exhibits a significantly decreased oxygen affinity (P50 = 20.8 mm Hg versus 5.8 mm Hg control; …

Identification Of Persistent Defects In Insulin Receptor Structure And Function In Capillary Endothelial Cells From Diabetic Rats, Ching Fai Kwok, Barry J. Goldstein, Dirk Muller-Wieland, Tian-Shing Lee, C. Ronald Kahn, George L. King

Department of Medicine Faculty Papers

Insulin actions and receptors were studied in capillary endothelial cells cultured from diabetic BB rats and their nondiabetic colony mates. The endothelial cells from diabetic rats of 2 mo duration had persistent biological and biochemical defects in culture. Compared with normal rats, endothelial cells from diabetic rats grew 44% more slowly. Binding studies of insulin and insulin-like growth factor I (IGF-I) showed that cells from diabetic rats had 50% decrease of insulin receptor binding (nondiabetic: 4.6 +/- 0.7; diabetic: 2.6 +/- 0.4% per milligram protein, P < 0.01), which was caused by a 50% decrease in the number of binding sites per milligram protein, whereas IGF-I binding was not changed. Insulin stimulation of 2-deoxy-glucose uptake and alpha-aminoisobutyric acid uptake were also severely impaired with a 80-90% decrease in maximal stimulation, in parallel with a 62% decrease in insulin-stimulated autophosphorylation (P < 0.05). ¹²⁵I-insulin cross-linking revealed an 140-kD alpha subunit of the insulin receptor similar to …

Passive Cation Transport In Reversibly Sickling Deer Red Blood Cells, Norma C. Adragna, Peter K. Lauf

Pharmacology and Toxicology Faculty Publications

No abstract provided.

Pentylenetetrazol-Induced Seizures In Pigeons And The Effects Of Ethosuximide Thereon, James J. Jakubow

Masters Theses

Previous research has shown that ethosuximide in high enough doses will disrupt operant responding in pigeons. Whether or not these same doses will protect against seizure activity in this species has not been previously tested.

To test this question a system for scoring pentylenetetrazol-induced seizures in pigeons was developed and the effects of ethosuximide on such seizures were evaluated. In Experiment 1 pentylenetetrazol at 15, 27 and 47 mg/kg reliably induced seizures. In Experiment 2 non-behaviorally active and behaviorally active doses of ethosuximide were tested for their seizure controlling effectiveness.

Doses of 20, 40, 80, 160 and 320 mg/kg ethosuximide …

Population Amalgamation And Genetic Variation: Observations On Artificially Agglomerated Tribal Populations Of Central And South America, R Chakraborty, P E Smouse, J V Neel

Faculty, Staff and Student Publications

The interpretation of data on genetic variation with regard to the relative roles of different evolutionary factors that produce and maintain genetic variation depends critically on our assumptions concerning effective population size and the level of migration between neighboring populations. In humans, recent population growth and movements of specific ethnic groups across wide geographic areas mean that any theory based on assumptions of constant population size and absence of substructure is generally untenable. We examine the effects of population subdivision on the pattern of protein genetic variation in a total sample drawn from an artificial agglomerate of 12 tribal populations …

Foraging Patterns Of Two Syngnathid Fishes: Importance Of Harpacticoid Copepods, Kevin Tipton, Susan S. Bell

Integrative Biology Faculty and Staff Publications

The diets of juvenile (mm) Syngnathus scovelli and of Hippocampus zosterae, abundant members of a resident fish community in a Thalassia testudnum seagrass bed in Tampa Bay, Florida, were examined from Apnl to October 1984. Harpacticoid copepods comprised most of the diet, both in terms of percent number and percent biomass, for the smaller size classes of S. scovelli and for H. zosterae, and harpacticoids generally had the highest index of relative importance (IRI) for both syngnathids. S. scovelli displayed ontogenetic switching to larger food items, such as amphipods, shrimp and crustacean eggs. Harpacticus sp. 1 was the …

1988 Spring No. 16, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 16 Spring 1988 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Dynamics Of The Aboveground And Belowground Structure Of The Seagrass Halodule Wrightii, Ruth A. Pangallo, Susan S. Bell

Integrative Biology Faculty and Staff Publications

Dynamics of the aboveground and belowground structure of the seagrass Halodule wrightii (Asherson) Asherson was studied from April 1984 to March 1985. Seasonality of plant structure was evident, with the highest abundance of short shoots and belowground structures occurring in June. An Inverse relationship between root and rhizome biomass was observed with the former reaching a maximum in February and March, while the latter attained highest values in July and August. The number of short shoots produced per branch varied over the year, ranging from a low of 0.4 to a high of 1.5. The ratio number of branches/rhizome length …

An Identified Histaminergic Neuron Can Modulate The Outputs Of Buccal-Cerebral Interneurons In Aplysia Via Presynaptic Inhibition, Hillel J. Chiel

Faculty Scholarship

We have identified 2 buccal-cerebral interneurons (BCIs), B17 and B18, that appear to be involved in the coordination of feeding behavior in Aplysia. The BCIs have their cell bodies in the buccal ganglion, but send axons to the cerebral ganglion via the cerebral-buccal connectives. The BCIs appear to make monosynaptic connections with neurons in the cerebral ganglion that modulate extrinsic muscles involved in feeding behavior. B17 and B18 are activated antiphasically during a motor program induced by stimulating the esophageal nerve and appear to 'read out' different phases of the buccal program to different cells in the cerebral ganglion. B17 …

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

1988 Winter No. 18, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 18 Winter1988-89 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 28 pages.

1988 Winter No. 15, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 15 Winter 1988 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

1988 Fall No. 17, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 17 Fall1988 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 32 pages.

1987 Fall No. 14, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 14 Fall 1987 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

1987 Spring No. 13, School Of Medicine Office Of Alumni Affairs

Medicinews

LSU Medicinews Number 13 Spring 1987 was edited by Russell C. Klein, and published by the LSU School of Medicine Office of Alumni and Continuing Education Services.

This issue consists of 24 pages.

Habitat Utilization By Harpacticoid Copepods: A Morphometric Approach, Susan S. Bell, Keith Walters, Margaret O. Hall

Integrative Biology Faculty and Staff Publications

Examination of harpacticoid copepod morphology was conducted to determine whether morphological resemblance provides a reasonable index of habltat utilization and movement. Discriminant analysis was used to explore the relationship between body form and habitat utilization for copepod species collected from 3 subhabitats within seagrass beds in Tampa Bay, Florida. To examine the accuracy of our procedure the discriminant function derived for Tarnpa Bay copepods was applied to copepods collected in worldwide studies. Three morphological characteristics - ratio of the length of Pereopod 1 first endopod segment to the remaining endopod segments, area of the cephalosome, and length of the first …