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Human Chorionic Gonadotropin Levels In Serum And Follicular Fluid Are Correlated With Body Mass Index Rather Than The Route Of Administration Of Purified Hcg In Art Cycles, Özlem Moralioğlu Üçkardeşler, Sevtap Kiliç, Gülnur Özakşi̇t, Çi̇ğdem Kunt, Ayça Işik, Beri̇l Yüksel, Leyla Mollamahmutoğlu

Turkish Journal of Medical Sciences

Aim: To investigate hCG levels in follicular fluid and serum after intramuscular (IM) or subcutaneous (SC) administration of purified hCG, and their association with oocyte maturation and body mass index (BMI) in women undergoing IVF-ET. Materials and Methods: The study included 160 infertile women that were recruited between April and June 2007. Standard-dose long IVF protocols were used for ovulation induction. SC or IM injections were used to administer hCG. BMI, oocyte maturity, and serum and follicular fluid hCG levels were the main outcome measurements. Results: hCG levels in serum and follicular fluid in the 2 groups were similar. A …

Hiv/Aids Knowledge Among A Group Of Youngsters Under Social Protection In Turkey, Veli̇ Duyan, Gülsüm Çamur Duyan

Turkish Journal of Medical Sciences

Aim: A number of radical changes in social conditions have created new hazards, such as STDs and HIV/AIDS, for young people. The purpose of this research was to determine the level of HIV/AIDS knowledge and information sources of the youngsters who are under social protection in Turkey. Materials and Methods: Participants of the study were 649 female and 739 males between the ages of 12 and 24 (M = 15.3, SD = 1.92) who are under social protection in Turkey. Data were collected using a demographic questionnaire, sources of HIV/AIDS information questionnaire, and HIV/AIDS questionnaire. The questionnaires were administered in …

Disseminated Herpes Zoster Infection In A Patient With Lymphoma, Behi̇ce Kurtaran, Semra Paydaş, Aslihan Candevi̇r, Süheyla Kömür, Hasan Sali̇h Zeki̇ Aksu

Turkish Journal of Medical Sciences

Immunosuppression facilitates varicella zoster virus (VZV) reactivation in immunocompromised patients. Atypical presentation such as disseminated disease of herpes zoster infection has been described in immunosuppressed patients. One of the best choices of therapy in herpes zoster infection is acyclovir, but long-term therapy may cause resistance to it. In this report a disseminated herpes zoster infection is described in a patient with T cell lymphoma after acyclovir/valacyclovir therapy. The infection was irresponsive to acyclovir and valacyclovir and we were unable to control the infection because it was not possible to obtain foscarnet and the patient died due to Staphylococcus epidermis sepsis.

The Effects Of Cigarette Smoking On Serum Oxidant Status, And Cholesterol, Homocysteine, Folic Acid, Copper, And Zinc Levels In University Students, İmge B. Ergüder, Asli Uçar, Işil Aritürk, Toker Ergüder, Aslihan Avci, Seni̇ha Hasi̇pek, Kadi̇rhan Sunguroğlu

Turkish Journal of Medical Sciences

Aim: To examine the effect of cigarette smoking on serum oxidative damage and oxidant status in university students. Materials and Methods: Subjects were randomly chosen from among Ankara University Faculty of Science students. The study was performed at the Ankara University Faculty of Health Sciences, Department of Nutrition and Dietetics, and the Ankara University Faculty of Medicine, Department of Biochemistry. In all, 44 volunteer (22 smokers and 22 non-smokers) students participated in the study. Malondialdehyde, sensitivity to oxidation (SO), and antioxidant potential (AOP), and total cholesterol, HDL cholesterol, LDL cholesterol, VLDL cholesterol, homocysteine, folic acid, copper, and zinc levels were …

Annexin A2, A7, And A11 Expression In Head And Neck Squamous Cell Carcinoma*, Sema Hücümenoğlu, Berrak Gümüşkaya, Murat Alper, Bülent Öcal, Erdem Karabulut

Turkish Journal of Medical Sciences

Aim: To investigate annexin A2 (ANXA2), annexin A7 (ANXA7), and annexin A11 (ANXA11) expression in head and neck squamous cell carcinoma (HNSCC). Although overexpression of ANXA2 has been reported in HNSCC, there are no studies on ANXA7 or ANXA11 in the same region. Materials and Methods: ANXA2, ANXA7, and ANXA11 were immunohistochemically studied in paraffin-embedded sections obtained from 43 patients with HNSCC. Results: ANXA2 and ANXA11 expression was more intense in the tumor areas in HNSCC (P = 0 for ANXA2, P = 0.05 for ANXA11). Both the tumors and normal mucosae showed membranous staining for ANXA7 and ANXA2, while …

An Analysis Of Adult Patients With Pulmonary And Extrapulmonary Tuberculosis*, Aygün Öztop, İpek Ünsal, Türkan Günay, Vi̇ldan Avkan Oğuz, Riza Çakmak

Turkish Journal of Medical Sciences

Aim: In this study, it was planned to compare the features of pulmonary and extra-pulmonary tuberculosis patients over age 15 years. Materials and methods: We compared 360 newly diagnosed pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) patients who were treated and followed up in Kahramanlar Tuberculosis Dispensary between 2000 and 2003, and were older than 15 years of age. The medical records of both patient groups were investigated in terms of age, sex, accompanying diseases, contact history and means of diagnosis. Results: The data were compared statistically between the 2 groups. Of these patients 78.1% were diagnosed with PTB while …

The Effects Of Adding Tramadol To Ropivacaine On Axillary Brachial Plexus Blockade In Uremic Patients*, Bayazit Di̇kmen, Mehmet Gamli, Eyüp Horasanli, Di̇lşen Örnek, Melekşah Pekel, Aydin Selçuk

Turkish Journal of Medical Sciences

Aim: To evaluate the effect of tramadol added to ropivacaine on quality of anesthesia, onset of sensory and motor blockade, duration of sensory and motor blockade, and first analgesic request time for axillary brachial plexus block in uremic patients. Materials and methods: After institutional approval and informed consent had been obtained, 45 ASA III uremic patients (age range 30-80) scheduled for arteriovenous fistula surgery were included in the study. Patients were randomly allocated into 2 groups. Axillary block was performed with a peripheral nerve stimulator by multiple injection technique. Patients in Group R (n = 23) received 38 mL of …

Short Term Outcomes Of The Use Of High Dose Intravenous Methylprednisolone For Acute Optic Neuritis In A Central Anatolian Population, Yasemi̇n Özdamar, Gölge Acaroğlu, Bayazit İlhan, Seyhan S. Özkan, Kuddusi̇ Teberi̇k

Turkish Journal of Medical Sciences

Aim: To describe the clinical characteristics and outcome of optic neuritis (ON) cases treated with high dose intravenous methylprednisolone (IVMP) in a tertiary referral clinic in Ankara, Turkey. Materials and methods: Consecutive patients in the past 5 years with acute isolated ON, who were treated with IVMP and had a follow-up of at least 3 months, were reviewed. Symptoms and findings, results of brain magnetic resonance imaging (MRI), etiologies, visual outcomes, and side effects of the treatment were evaluated. Results: Etiological causes could be elicited in 57 (85.1%) of 67 patients; the most common of which was multiple sclerosis (MS). …

Burnout At A Bone Marrow Transplantation Unit In Turkey: Effects Of Interactive Psychoeducational Seminars, Ayşegül Yilmaz, Ati̇lla Soykan, Tuğba Ayaz, Elmas İnce Kale, Günhan Gürman, Hakan Kumbasar

Turkish Journal of Medical Sciences

Aim: Health staff working at bone marrow transplantation units (BMTUs) is found to be at a higher risk for suffering from burnout. This paper reports a critical assessment of how burnout level of the health staff working at a bone marrow transplantation unit changes after psychoeducational seminars. Materials and methods: The study was conducted by the Consultation Liaison Psychiatry Division at the BMTU of the Department of Hematology at Ankara University, School of Medicine. Sixty-one health staff members (67%) working at the BMTU filled out socio-demographic and occupational data form, and validated Turkish versions of Maslach Burnout Inventory (MBI), Hospital …

Humoral Immunity And Haemoglobin F (Hbf) Status In Steady State Adult Nigerian Sickle Cell Disease Patients With Asymptomatic Malaria, John Ayodele Olaniyi, Olatubosun Ganiyu Arinola

Turkish Journal of Medical Sciences

Aim: Nigeria, where malaria is prevalent, has the largest sickle cell gene pool in the world. To this end, there is a need for increased understanding of the pathophysiology of HbSS patients in a malaria endemic zone to reduce mortality. Haematology Department, University College Hospital, Ibadan, Nigeria. Materials and methods: The levels of HbF, IgG, IgA, IgM, transferin (TRF), C-reactive protein (CRP), and haptoglobin (HPT) were determined in 14 Nigerians having HbAA genotype with malaria parasitaemia (HbAA +MP), 17 Nigerians having haemoglobin AA genotype without malaria parasitaemia (HbAA -MP), 15 Nigerians having haemoglobin SS genotype without malaria parasitaemia (HbSS -MP), …

Evaluation And Follow-Up Of Cases Diagnosed As Child Abuse And Neglect At A Tertiary Hospital In Turkey, Ülkü Tiraş, Di̇lek Di̇lli̇, Yildiz Dallar, Resmi̇ye Oral

Turkish Journal of Medical Sciences

Aim: Data on child maltreatment in Turkey would appear to be scarce. This manuscript aims to address the prevalence and severity of child maltreatment in Turkey. Materials and methods: We report on 215 cases at the Ministry of Health’s Ankara Training and Research Hospital that were followed-up by the multidisciplinary Child Protection Team (CPT) between 1 January 2001 and 31 December 2005. The data collected on subjects included characteristics of the victims and the perpetrators, family demographics, maltreatment types, medical findings, mortality rate, and outcomes for the victim and the perpetrator. Results: Age and gender distribution of the victims were …

Treatment Options For Neuropathic Pain, Sara Akre

Honors Program Theses

Neuropathic pain is caused by injury to or disease of the nervous system. It affects many people around the world and is a symptom of several diseases. In the United States, it is estimated that up to 3% of Americans have neuropathic pain, which costs the healthcare system $40 billion annually (Gilron et al. 2006). It is, unfortunately, difficult to ameliorate or eliminate due to the complexity of the nervous system and its plasticity and is, therefore, a chronic condition. Health care professionals continually struggle to treat patients' pain and, since tolerance to painkillers is easily built up, this often …

Expression Of Hpv16 E5 Produces Enlarged Nuclei And Polyploidy Through Endoreplication, Lulin Hu, Tamara A. Potapova, Shibo Li, Susannah Rankin, Gary J. Gorbsky, Peter C. Angeletti, Brian P. Ceresa

Nebraska Center for Virology: Faculty Publications

Anogenital cancers and head and neck cancers are causally-associated with infection by high-risk

human papillomavirus (HPV). The mechanism by which high-risk HPVs contribute to

oncogenesis is poorly understood. HPV16 encodes three genes (HPV16 E5, E6, and E7) that can

transform cells when expressed independently. HPV16 E6 and E7 have well-described roles

causing genomic instability and unregulated cell cycle progression. The role of HPV16 E5 in cell

transformation remains to be elucidated. Expression of HPV16 E5 results in enlarged, polyploid

nuclei that are dependent on the level and duration of HPV16 E5 expression. Live-cell imaging

data indicate these changes do not …

Human Papillomavirus 16 E5 Induces Bi-Nucleated Cell Formation By Cell-Cell Fusion, Lulin Ha, Kendra Plafker, Valeriya Vorozhoko, Rosemary E. Zuna, Marie H. Hanigan, Gary J. Gorbsky, Scott M. Plafker, Peter C. Angeletti, Brian P. Ceresa

Nebraska Center for Virology: Faculty Publications

Human Papillomaviruses (HPV) 16 is a DNA virus encoding three oncogenes – E5, E6, and E7. The E6 and E7 proteins have well-established roles as inhibitors of tumor suppression, but the contribution of E5 to malignant transformation is controversial. Using spontaneously immortalized human keratinocytes (HaCaT cells), we demonstrate that expression of HPV16 E5 is necessary and sufficient for the formation of bi-nucleated cells, a common characteristic of precancerous cervical lesions. Expression of E5 from non-carcinogenic HPV6b does not produce bi-nucleate cells. Video microscopy and biochemical analyses reveal that bi-nucleates arise through cell-cell fusion. Although most E5-induced bi-nucleates fail to propagate, …

Regulation Of Innate Immune Responses By Bovine Herpesvirus 1 And Infected Cell Protein 0 (Bicp0), Clinton Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BoHV-1) infected cell protein 0 (bICP0) is an important transcriptional regulatory protein that stimulates productive infection. In transient transfection assays, bICP0 also inhibits interferon dependent transcription. bICP0 can induce degradation of interferon stimulatory factor 3 (IRF3), a cellular transcription factor that is crucial for activating beta interferon (IFN-β) promoter activity. Recent studies also concluded that interactions between bICP0 and IRF7 inhibit trans-activation of IFN-β promoter activity. The C3HC4 zinc RING (really important new gene) finger located near the amino terminus of bICP0 is important for all known functions of bICP0. A recombinant virus that contains a single …

Use Of Average Mutual Information For Studying Changes In Hiv Populations, Khalid Sayood, Federico Hoffman, Charles Wood

Nebraska Center for Virology: Faculty Publications

Average mutual information (AMI) has been used in a number of applications in bioinformatics. In this paper we present its use to study genetic changes in populations; in particular populations of HIV viruses. Disease progression of HIV-1 infection in infants can be rapid resulting in death within the the first year, or slow, allowing the infant to survive beyond the first year. We study the development of rapid and slow progressing HIV population using AMI charts based on average mutual information among amino acids in the env gene from a population of 1142 clones derived from seven infants with slow …

Protection Against Mucosal Shiv Challenge By Peptide And Helper-Dependent Adenovirus Vaccines, Eric A. Weaver, Pramod N. Nehete, Bharti P. Nehete, Stephanie J. Buchl, Donna Palmer, David C. Montefiori, Philip Ng, K. Jagannadha Sastry, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Groups of rhesus macaques that had previously been immunized with HIV-1 envelope (env) peptides and first generation adenovirus serotype 5 (FG-Ad5) vaccines expressing the same peptides were immunized intramuscularly three times with helper- dependent adenovirus (HD-Ad) vaccines expressing only the HIV-1 envelope from JRFL. No gag, pol, or other SHIV genes were used for vaccination. One group of the FG-Ad5- immune animals was immunized three times with HD-Ad5 expressing env. One group was immunized by serotype-switching with HD-Ad6, HD-Ad1, and HD-Ad2 expressing env. Previous work demonstrated that serum antibody levels against env were significantly higher in the serotype-switched group than …

Pyramidobacter Piscolens Gen. Nov., Sp. Nov., A Member Of The Phylum ‘Synergistetes’ Isolated From The Human Oral Cavity, Julia Downes, Sonia R. Vartoukian, Floyd E. Dewhirst, Jacques Izard, Tsute Chen, Wen-Han Yu, Iain C. Sutcliffe, William G. Wade

Department of Food Science and Technology: Faculty Publications

Four strains of anaerobic, gram-negative bacilli isolated from the human oral cavity were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group distinct from any species with validly published names. 16S rRNA and 23S rRNA gene sequence analyses and DNA–DNA reassociation data revealed that the strains constituted a novel group within the phylum ‘Synergistetes’ and were most closely related to Jonquetella anthropi. Two libraries of randomly cloned DNA were prepared from strain W5455T and were sequenced to provide a genome survey as a resource for metagenomic studies. A new genus …

Regulation Of Low Density Lipoprotein Receptor Splicing Efficiency, I-Fang Ling

University of Kentucky Doctoral Dissertations

Low density lipoprotein receptor (LDLR) is an apolipoprotein E (apoE) receptor and may play a role in Alzheimer’s disease (AD) development. A single nucleotide polymorphism (SNP), rs688, that has been identified to modulate the splicing efficiency of LDLR exon 12 and is associated with higher cholesterol and AD in some case-control populations. The exon 12 deleted mRNA is predicted to produce a soluble form of LDLR that fails to mediate apoE uptake. To gain additional insights, in this study, I seek to understand the regulation of LDLR splicing efficiency. To identify functional cis-elements within LDLR exon 12, I mutated several …

Liquid Chromatography-Tandem Mass Spectrometry For The Determination Of Methylprednisolone In Rat Plasma And Liver After Intravenous Administration Of Its Liver-Targeted Dextran Prodrug, Shuang-Qing Zhang, Helen R. Thorsheim, Suman Penugonda, Venkateswaran C. Pillai, Quentin R. Smith, Reza Mehvar

Pharmacy Faculty Articles and Research

A specific and sensitive liquid chromatography (LC)-tandem mass spectrometric method for quantitative determination of methylprednisolone (MP) in rat plasma and liver was developed and validated using triamcinolone acetonide as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma and liver. The separation of MP was performed on a C(18) column with a mobile phase of acetonitrile:0.5% formic acid aqueous solution (85:15, v/v) over 4 min. The assay was based on the selected reaction monitoring transitions at m/z 375 -> 161 for MP in plasma, 375 -> 357 for …

Control Of Myosin Motor Activity And Actin Filament Translation By Alteration Of Assay Reducing Potential, Siva Krishna Nalabotu

Theses, Dissertations and Capstones

The use of biomolecular motors for nanotechnological applications has been impaired by an inability to control biomolecular motor activity in a temporal fashion. Previous data has suggested that myosin activity can be modulated by inducing changes in the degree of myosin oxidation. The hypothesis of this study is that myosin motor activity can be regulated by altering the amount of antioxidants and reducing agents present in assay solution. To test this hypothesis we systematically altered the concentration of reducing agents and examined the effects of these changes on myosin-dependent actin filament translation. Our findings indicate that myosin activity can be …

Neural Substrates Of Sound–Touch Synesthesia After A Thalamic Lesion, Michael S. Beauchamp, Tony Ro

Publications and Research

Neural plasticity induced by stroke can mediate positive outcomes, such as recovery of function, but can also result in the formation of abnormal connections with negative consequences for perception and cognition. In three experiments using blood-oxygen level dependent (BOLD) functional magnetic resonance imaging, we examined the neural substrates of acquired auditory-tactile synesthesia, in which certain sounds can produce an intense somatosensory tingling sensation in a patient with a thalamic lesion. Compared with nine normal controls, the first experiment showed that the patient had a threefold greater BOLD response to sounds in the parietal operculum, the location of secondary somatosensory cortex. …

Borderline Personality Disorder: An Overview Of History, Diagnosis And Treatment In Adolescents, Linah Al-Alem, Hatim A. Omar

Pharmacology and Nutritional Sciences Faculty Publications

Borderline personality disorder (BPD) is a cluster B personality disorder. It is characterized by erratic behaviors, emotional instability and one of its hallmarks is self injurious behavior, which starts in adolescence. Patients with BPD are difficult to treat, most have a history of child sexual abuse, about a quarter present with sexual abuse from a caretaker. Although personality disorders are diagnosed only in adults, BPD manifests itself in adolescence in the form of uncontrollable anger, self mutilations, dissociation and other such behaviors. Hence, there is a growing number of scientists discussing the possibility of diagnosing BPD in adolescents. Here, we …

Meta-Analysis: Prophylactic Drainage And Bleeding Complications In Thyroid Surgery, Steven A. Kennedy, Robert A. Irvine, Brian D. Westerberg, Hongbin Zhang

Publications and Research

Objective: To conduct a comprehensive systematic review and high-quality meta-analysis to determine whether prophylactic drain placement reduces adverse bleeding events in thyroid surgery.

Data Sources: MEDLINE (OVID and PubMed), CENTRAL, CDSR, ACP Journal Club, DARE, EMBASE, PREMEDUNE, OLDMEDLINE, CINAHL, BIOSIS Previews, LILACS, KOREAMED, SAMED, IndMED, SIGLE, ScienceDirect, and INGENTACONNECT.

Review Methods: Studies for evaluation included all prospective trials assessing the use of drainage in thyroid surgery. We excluded case studies, retrospective studies, reviews, and studies that had a "selective" method of postoperative drainage that was not defined or was based on surgeon preference. Search strategies were broad and …

Upregulation Of Heme Oxygenase-1 Combined With Increased Adiponectin Lowers Blood Pressure In Diabetic Spontaneously Hypertensive Rats Through A Reduction In Endothelial Cell Dysfunction, Apoptosis And Oxidative Stress, Jian Cao, George Drummond, Kazuyoshi Inoue, Komal Sodhi, Xiao Ying Li, Shinji Omura

Pharmaceutical Science and Research

This study was designed to investigate the effect of increased levels of HO-1 on hypertension exacerbated by diabetes. Diabetic spontaneously hypertensive rat (SHR) and WKY (control) animals were treated with streptozotocin (STZ) to induce diabetes and stannous chloride (SnCl2) to upregulate HO-1. Treatment with SnCl2 not only attenuated the increase of blood pressure (p<0.01), but also increased HO-1 protein content, HO activity and plasma adiponectin levels, decreased the levels of superoxide and 3-nitrotyrosine (NT), respectively. Reduction in oxidative stress resulted in the increased expression of Bcl-2 and AKT with a concomitant reduction in circulating endothelial cells (CEC) in the peripheral blood (p<0.005) and an improvement of femoral reactivity (response to acetylcholine). Thus induction of HO-1 accompanied with increased plasma adiponectin levels in diabetic hypertensive rats alters the phenotype through a reduction in oxidative stress, thereby permitting endothelial cells to maintain an anti-apoptotic environment and the restoration of endothelial responses thus preventing hypertension.

Studies To Characterize The Requirements For The Binding And Release Of Erdj3, A Mammalian Er Dnaj Homolog, From Substrates, Yi Jin

Theses and Dissertations (ETD)

Heat shock protein 70s (Hsp70s) and their DnaJ co-factors exist in all organisms and in all eukaryotic organelles. These highly conserved chaperone pairs facilitate a large number of cellular processes. ERdj3 was identified as a soluble, lumenal DnaJ family member that binds to unassembled immunoglobulin heavy chains (HC) along with the BiP chaperone complex in the endoplasmic reticulum of mammalian cells. Here we demonstrate that ERdj3 binds directly to two unfolded substrates: immunoglobulin γHC and denatured firefly luciferase. Using mutagenesis studies on ERdj3 in both in vivo and in vitro binding assays, we defined ERdj3’s critical amino acids in domain …

The Role Of Gtp In Transient Splitting Of 70s Ribosomes By Rrf (Ribosome Recycling Factor) And Ef-G (Elongation Factor G)., Go Hirokawa, Nobuhiro Iwakura, Akira Kaji, Hideko Kaji

Department of Biochemistry and Molecular Biology Faculty Papers

Ribosome recycling factor (RRF), elongation factor G (EF-G) and GTP split 70S ribosomes into subunits. Here, we demonstrated that the splitting was transient and the exhaustion of GTP resulted in re-association of the split subunits into 70S ribosomes unless IF3 (initiation factor 3) was present. However, the splitting was observed with sucrose density gradient centrifugation (SDGC) without IF3 if RRF, EF-G and GTP were present in the SDGC buffer. The splitting of 70S ribosomes causes the decrease of light scattering by ribosomes. Kinetic constants obtained from the light scattering studies are sufficient to account for the splitting of 70S ribosomes …

Genetic Counselling And Testing For Inherited Gene Mutations In Newly Diagnosed Patients With Breast Cancer: A Review Of The Existing Literature And A Proposed Research Agenda, Bettina Meiser, Kathy Tucker, Michael Friedlander, Kristine Barlow-Stewart, Elizabeth Lobb, Christobel Saunders, Gillian Mitchell

Research outputs pre 2011

Many women newly diagnosed with breast cancer and with a strong family history of breast cancer are referred to a family cancer service for genetic counselling and for consideration of genetic testing for germline mutations in cancer predisposition genes following completion of their cancer treatment. However, there is growing evidence that mutation status may influence treatment recommendations, and that there may be benefits in having 'treatment-focused genetic counselling and testing' available shortly after cancer diagnosis. This article reviews the literature that could inform the development of treatment-focused genetic counselling and testing, including: the rationale for genetic testing to aid with …

Stromal Gene Signatures In Large-B-Cell Lymphomas., G Lenz, G Wright, S S Dave, W Xiao, J Powell, H Zhao, W Xu, B Tan, N Goldschmidt, Javeed Iqbal, Julie M. Vose, M Bast, Kai Fu, D D. Weisenburger, T C Greiner, James O. Armitage, A Kyle, L May, R D Gascoyne, J M Connors, G Troen, H Holte, S Kvaloy, D Dierickx, G Verhoef, J Delabie, E B Smeland, P Jares, A Martinez, A Lopez-Guillermo, E Montserrat, E Campo, R M Braziel, T P Miller, L M Rimsza, J R Cook, B Pohlman, J Sweetenham, R R Tubbs, R I Fisher, E Hartmann, A Rosenwald, G Ott, H-K Muller-Hermelink, D Wrench, T A Lister, E S Jaffe, W H Wilson, W C. Chan, L M Staudt

Journal Articles: Pathology and Microbiology

BACKGROUND: The addition of rituximab to combination chemotherapy with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), or R-CHOP, has significantly improved the survival of patients with diffuse large-B-cell lymphoma. Whether gene-expression signatures correlate with survival after treatment of diffuse large-B-cell lymphoma is unclear.

METHODS: We profiled gene expression in pretreatment biopsy specimens from 181 patients with diffuse large-B-cell lymphoma who received CHOP and 233 patients with this disease who received R-CHOP. A multivariate gene-expression-based survival-predictor model derived from a training group was tested in a validation group.

RESULTS: A multivariate model created from three gene-expression signatures--termed "germinal-center B-cell," "stromal-1," and "stromal-2"--predicted …

Androgen-Independent Prostate Cancer Cells Acquire The Complete Steroidogenic Potential Of Synthesizing Testosterone From Cholesterol., Paulette R. Dillard, Ming-Fong Lin, Shafiq A. Khan

Journal Articles: Biochemistry & Molecular Biology

The proliferation and differentiation of normal prostate epithelial cells depends upon the action of androgens produced by the testis. Prostate cancers retain the ability to respond to androgens in the initial stages of cancer development, but progressively become independent of exogenous androgens in advanced stages of the disease while maintaining the expression of functional androgen receptor (AR). In the present study, we have determined the potential of prostate cancer cells to synthesize androgens from cholesterol which may be involved in intracrine regulation of AR in advanced stages of the disease. Established androgen-independent prostate cancer cell lines, PC3 and DU145 cells, …