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Scas Officers And Council Members 2011 - 2012

Journal of the South Carolina Academy of Science

No abstract provided.

Blood-Brain Barrier Abnormalities Caused By Hiv-1 Gp120: Mechanistic And Therapeutic Implications., Jean-Pierre Louboutin, David S Strayer

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

The blood-brain barrier (BBB) is compromised in many systemic and CNS diseases, including HIV-1 infection of the brain. We studied BBB disruption caused by HIV-1 envelope glycoprotein 120 (gp120) as a model. Exposure to gp120, whether acute [by direct intra-caudate-putamen (CP) injection] or chronic [using SV(gp120), an experimental model of ongoing production of gp120] disrupted the BBB, and led to leakage of vascular contents. Gp120 was directly toxic to brain endothelial cells. Abnormalities of the BBB reflect the activity of matrix metalloproteinases (MMPs). These target laminin and attack the tight junctions between endothelial cells and BBB basal laminae. MMP-2 and …

Kaempferol Nanoparticles Achieve Strong And Selective Inhibition Of Ovarian Cancer Cell Viability., Haitao Luo, Bingbing Jiang, Bingyun Li, Zhaoliang Li, Bing-Hua Jiang, Yi Charlie Chen

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Ovarian cancer is one of the leading causes of cancer death for women throughout the Western world. Kaempferol, a natural flavonoid, has shown promise in the chemoprevention of ovarian cancer. A common concern about using dietary supplements for chemoprevention is their bioavailability. Nanoparticles have shown promise in increasing the bioavailability of some chemicals. Here we developed five different types of nanoparticles incorporating kaempferol and tested their efficacy in the inhibition of viability of cancerous and normal ovarian cells. We found that positively charged nanoparticle formulations did not lead to a significant reduction in cancer cell viability, whereas nonionic polymeric nanoparticles …

Temporal Changes In Innate Immune Signals In A Rat Model Of Alcohol Withdrawal In Emotional And Cardiorespiratory Homeostatic Nuclei., Kate Freeman, Anthony Brureau, Rajanikanth Vadigepalli, Mary M Staehle, Melanie M Brureau, Gregory E Gonye, Jan B Hoek, D Craig Hooper, James S Schwaber

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: Chronic alcohol use changes the brain's inflammatory state. However, there is little work examining the progression of the cytokine response during alcohol withdrawal, a period of profound autonomic and emotional upset. This study examines the inflammatory response in the central nucleus of the amygdala (CeA) and dorsal vagal complex (DVC), brain regions neuroanatomically associated with affective and cardiorespiratory regulation in an in vivo rat model of withdrawal following a single chronic exposure.

METHODS: For qRT-PCR studies, we measured the expression of TNF-α, NOS-2, Ccl2 (MCP-1), MHC II invariant chain CD74, and the TNF receptor Tnfrsf1a in CeA and DVC …

Faculty Publications 2011, Lsu Health Sciences Center New Orleans

Historical Faculty Publications

This Faculty bibliography contains 812 publications.

The Emerging Role Of Acyl-Coa Thioesterases And Acyltransferases In Regulating Peroxisomal Lipid Metabolism, Mary Hunt, Marina Siponen, Stefan Alexson

Articles

The importance of peroxisomes in lipid metabolism is now well established and peroxisomes contain approximately 60 enzymes involved in these lipid metabolic pathways. Several acyl-CoA thioesterase enzymes (ACOTs) have been identified in peroxisomes that catalyze the hydrolysis of acyl-CoAs (short-, medium-, long- and very long-chain), bile acid-CoAs, and methyl branched-CoAs, to the free fatty acid and coenzyme A. A number of acyltransferase enzymes, which are structurally and functionally related to ACOTs, have also been identified in peroxisomes, which conjugate (or amidate) bile acid-CoAs and acyl-CoAs to amino acids, resulting in the production of amidated bile acids and fatty acids. The …

Signals From Intraventricular Depth Electrodes Can Control A Brain-Computer Interface, Jerry J. Shih, Dean J. Krusienski

Electrical & Computer Engineering Faculty Publications

A Brain-Computer Interface (BCI) is a device that enables severely disabled people to communicate and interact with their environments using their brain waves. Most research investigating BCI in humans have used scalp-recorded electroencephalography (EEG). We have recently demonstrated that signals from intracranial electrocorticography (ECoG) and stereotactic depth electrodes (SDE) in the hippocampus can be used to control a BCI P300 Speller paradigm. We report a case in which stereotactic depth electrodes positioned in the ventricle were able to obtain viable signals for a BCI. Our results demonstrate that event-related potentials from intraventricular electrodes can be used to reliably control the …

Thoracic Epidural Anesthesia With Bupivacaine Attenuates Systemic Inflammatory Response And Lung Impairment Associated With Cerulein-Induced Pancreatitis In Rats, Nurdan Bedi̇rli̇, Nalan Akyürek, Bahadir Kösem, Melek Yaman, Aslihan Çavunt Bayraktar, Ömer Kurti̇pek, Mustafa Kavutçu

Turkish Journal of Medical Sciences

To evaluate the effects of thoracic epidural anesthesia (TEA) on acute pancreatitis (AP)-induced lung injury in a rat model. Materials and methods: A total of 24 rats were divided into 3 groups: the control group (n = 8; pancreatitis), the bupivacaine group (n = 8; pancreatitis + 20 µL/h 0.5% bupivacaine), and the saline group (n = 8, pancreatitis + 20 µL/h saline). Pancreatitis was induced by 4 intraperitoneal injections of 50 µg/kg cerulein. At 24 h, blood was collected for determination of amylase, blood gas, tumor necrosis factor-\alpha (TNF-\alpha), interleukin (IL)-6, and IL-1\beta; lung samples were collected for the …

Seroprevalence Of Major Blood-Borne Infections Among Blood Donors In Kano, Nigeria, Emmanuel Nwankwo, Imoru Momodu, Ismaila Umar, Babashani Musa, Solomon Adeleke

Turkish Journal of Medical Sciences

To examine the seroprevalence of common blood infections among blood donors, which remains a major concern in transfusion medicine in Nigeria. Blood transfusion is one of the known therapeutic interventions that cut across a number of clinical disciplines. Materials and methods: The seroprevalences of human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis B surface antigen (HBsAg), and syphilis among 280 blood donors at the Murtala Mohammed Specialist Hospital (MMSH) were determined. The seroprevalences of the pathogens were determined with regard to age, sex, donor category, and occurrence of association pathogens. Between June and December 2008, 276 males (98.6%) and …

Violence Among High School Students In Malatya: A Prevalence Study, Osman Celbi̇ş, Leyla Karaoğlu, Mücahi̇t Eğri̇, Bora Özdemi̇r

Turkish Journal of Medical Sciences

To determine the prevalence of violence-related behaviors on school property and to identify the predictors of youth violence among high school adolescents in Malatya. Materials and methods: Using a cluster sampling method, a cross-sectional descriptive study was carried out involving 1175 students. Backward logistic regression analysis was performed to evaluate the independent association existing between the potential risk factors and violence-related behaviors. Results: Of the students, 24.5% had been in a physical fight, 5.5% were injured and had to be treated by a doctor or nurse, and 4.3% had carried a weapon on school property during the 12 months preceding …

Outcomes In 12 Gynecologic Cancer Patients With Brain Metastasis: A Single Center’S Experience, Esra Çabuk Cömert, Tevfi̇k Berk Bi̇ldaci, Burcu Kisa Karakaya, Nefi̇se Çağla Tarhan, Özlem Özen, Sali̇h Gülşen, Polat Dursun, Ali̇ Ayhan

Turkish Journal of Medical Sciences

To present 12 gynecologic cancer cases with brain metastasis and a discussion of the relevant literature. Gynecologic malignancy is the second most common cancer in elderly women, following breast cancer. These cancers usually spread locally at first, and common distant metastatic sites are the lungs, liver, spleen, and distant lymph nodes. The brain is not a usual site of metastatic involvement. Materials and methods: The study included 12 cases with various gynecologic malignancies that were retrospectively analyzed. Therapeutic modalities, survival, and time between initial surgery and brain metastasis were recorded. Results: The mean survival was 41.4 months in 6 patients …

The Role Of Quantitative D-Dimer Levels In The Follow-Up And Differential Diagnosis Of Pulmonary Thromboembolism And Community-Acquired Pneumonia, Ayhan Varol, Nurdan Köktürk, Hati̇ce Kiliç, Müge Aydoğdu, Numan Nadi̇r Eki̇m

Turkish Journal of Medical Sciences

Aim: To identify the quantitative D-dimer levels in pulmonary thromboembolism (PTE) and hospitalized-community acquired pneumonia (CAP) patients, and to determine the alteration of D-dimer levels with anticoagulant or antibacterial therapy. It is not well known that the quantitative analysis of D-dimer has a role in the differential diagnosis of PTE from other diseases. Materials and methods: Serum D-dimer levels were measured and compared prospectively with a latex-enhanced immunoturbidimetric method at admission, before initiating any antibiotic or anticoagulant therapy and then at days 3, 10, and 30 of treatment in PTE and CAP patients. Results: A total of 80 patients (45 …

Evaluation Of Vitamin B-12 Level In Middle-Aged Obese Women With Metabolic And Non-Metabolic Syndrome: Case-Control Study, Davut Baltaci, Ali̇ Kutlucan, Serkan Öztürk, İsmai̇l Karabulut, Hayri̇ye Ak Yildirim, Ahmet Çeler, Gökhan Celbek, İsmai̇l Hamdi̇ Kara

Turkish Journal of Medical Sciences

To investigate the correlation between vitamin B12 and body mass index (BMI) along with insulin resistance (IR) in middle-aged obese women. Materials and methods: The study was designed as a case-control study. The study group included middle-aged obese women and the control group included aged-matched lean women. Weight, height, and hip and waist circumferences were measured. Biochemical parameters such as fasting and postprandial glucose, vitamin B12 and folic acid levels, and lipid profiles were assayed. Results: Enrolled in the study were 116 middle-aged obese and 103 aged-matched healthy lean women. The vitamin B12 level of the obese women was significantly …

Effects Of Etomidate On Blood Cortisol, Insulin, And Glucose Levels And Ponv Rates In Smokers, Cevdet Sümer, Ömer Lütfi̇ Erhan, Ayşe Beli̇n Özer, Fuat Yildiz

Turkish Journal of Medical Sciences

To observe differences in the effects of etomidate, used for anesthesia induction, on surgery-induced endocrine response and postoperative nausea and vomiting (PONV) among nonsmokers and smokers. Materials and methods: Eighty adult patients with ASA I-II risk status were randomly divided into 2 groups: smokers (Group I, n = 40) and nonsmokers (Group II, n = 40). Patients were then further divided into subgroups according to the drug used for anesthesia induction, either propofol (Group IP and Group IIP) or etomidate (Group IE and Group IIE). Blood cortisol, insulin, glucose, and white blood cell (WBC) levels were measured and PONV scores …

Improving Electrocardiogram Interpreting Skills Among Primary Care Physicians In Turkey, Turan Set, Zekeri̇ya Aktürk, Mutlu Büyüklü, Zeli̇ha Cansever, Ümmü Zeynep Avşar, Ümi̇t Avşar, Memet Işik, Hami̇t Acemoğlu

Turkish Journal of Medical Sciences

To investigate the improvement in electrocardiogram (ECG) interpreting skills after a standardized ECG interpreting program as a continuous medical education activity among primary care physicians. Materials and methods: The study was conducted in Turkey between December 2006 and October 2010. Out of the 81 provinces in Turkey, 17 were randomly selected and all physicians were invited to take the course on a voluntary basis and in total 798 physicians agreed to participate. The course was a half-day program. A test was applied to the participants before and after the training. The results of 781 participants were analyzed. Results: Of the …

Structural Characterization Of A Conserved, Calcium-Dependent Periplasmic Protease From Legionella Pneumophila, Debashree Chatterjee, Chelsea D. Boyd, George A. O'Toole, Holger Sondermann

Dartmouth Scholarship

The bacterial dinucleotide second messenger c-di-GMP has emerged as a central molecule in regulating bacterial behavior, including motility and biofilm formation. Proteins for the synthesis and degradation of c-di-GMP and effectors for its signal transmission are widely used in the bacterial domain. Previous work established the GGDEF-EAL domain-containing receptor LapD as a central switch in Pseudomonas fluorescens cell adhesion. LapD senses c-di-GMP inside the cytosol and relays this signal to the outside by the differential recruitment of the periplasmic protease LapG. Here we identify the core components of an orthologous system in Legionella pneumophila. Despite only moderate sequence conservation at …

Complementary And Integrative Oncology In The Cross-Cultural Region Of The Middle East And South Asia., Eran Ben-Arye, Barrie Cassileth, Peter Heusser, Fatma Afifi, Bashar Saad, Senthamil R Selvan

Department of Medical Oncology Faculty Papers

No abstract provided.

Lineage-Specific Differences Between Human And Simian Immunodeficiency Virus Regulation Of Gp120 Trimer Association And Cd4 Binding, Andrés Finzi, Beatriz Pacheco, Shi-Hua Xiang, Marie Pancera, Alon Herschhorn, Liping Wang, Xing Zeng, Anik Desormeaux, Peter D. Kwong, Joseph Sodroski

Nebraska Center for Virology: Faculty Publications

Metastable conformations of the gp120 and gp41 envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) must be maintained in the unliganded state of the envelope glycoprotein trimer. Binding of gp120 to the primary receptor, CD4, triggers the transition to an open conformation of the trimer, promoting interaction with the CCR5 chemokine receptor and ultimately leading to gp41-mediated virus-cell membrane fusion and entry. Topological layers in the gp120 inner domain contribute to gp120-trimer association in the unliganded state and to CD4 binding. Here we describe similarities and differences between HIV-1 and SIVmac gp120. In both …

Comparative Analysis Of The Fusion Efficiency Elicited By The Envelope Glycoprotein V1–V5 Regions Derived From Human Immunodeficiency Virus Type 1 Transmitted Perinatally, Hongyan Guo, Levon Abrahamyan, Chang Liu, Mackenzie Waltke, Yunqi Geng, Qimin Chen, Charles Wood, Xiaohong Kong

Nebraska Center for Virology: Faculty Publications

Understanding the properties of viruses preferentially establishing infection during perinatal transmission of human immunodeficiency virus type 1 (HIV-1) is critical for the development of effective measures to prevent transmission. A previous study demonstrated that the newly transmitted viruses (in infants) of chronically infected mother–infant pairs (MIPs) were fitter in terms of growth, which was imparted by their envelope (Env) glycoprotein V1–V5 regions, than those in the corresponding chronically infected mothers. In order to investigate whether the higher fitness of transmitted viruses was conferred by their higher entry efficiency directed by the V1–V5 regions during perinatal transmission, the fusogenicity of Env …

Fusion Of A Fluorescent Protein To The Pul25 Minor Capsid Protein Of Pseudorabies Virus Allows Live-Cell Capsid Imaging With Negligible Impact On Infection, Kevin P. Bohannon, Patricia J. Sollars, Gary E. Pickard, Gregory A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

In order to resolve the location and activity of submicroscopic viruses in living cells, viral proteins are often fused to fluorescent proteins (FPs) and visualized by microscopy. In this study, we describe the fusion of FPs to three proteins of pseudorabies virus (PRV) that allowed imaging of capsids in living cells. Included in this study are the first recombinant PRV strains expressing FP–pUL25 fusions based on a design applied to herpes simplex virus type 1 by Homa and colleagues. The properties of each reporter virus were compared in both in vitro and in vivo infection models. PRV strains expressing FP–pUL25 …

Molecular Genetic And Biochemical Characterization Of The Vaccinia Virus I3 Protein, The Replicative Single-Stranded Dna Binding Protein, Matthew D. Greseth, Kathleen A. Boyle, Matthew S. Bluma, Bethany Unger, Matthew S. Wiebe, Jamaria A. Soares-Martins, Nadi T. Wickramasekera, James Wahlberg, Paula Traktman

Nebraska Center for Virology: Faculty Publications

Vaccinia virus, the prototypic poxvirus, efficiently and faithfully replicates its approximately 200-kb DNA genome within the cytoplasm of infected cells. This intracellular localization dictates that vaccinia virus encodes most, if not all, of its own DNA replication machinery. Included in the repertoire of viral replication proteins is the I3 protein, which binds to single-stranded DNA (ssDNA) with great specificity and stability and has been presumed to be the replicative ssDNA binding protein (SSB). We substantiate here that I3 colocalizes with bromodeoxyuridine (BrdU)-labeled nascent viral genomes and that these genomes accumulate in cytoplasmic factories that are delimited by membranes derived from …

An Epidemiological Model Of Rift Valley Fever With Spatial Dynamics, Tianchan Niu, Holly D. Gaff, Yiannis E. Papelis, David M. Hartley

Biological Sciences Faculty Publications

As a category A agent in the Center for Disease Control bioterrorism list, Rift Valley fever (RVF) is considered a major threat to the United States (USA). Should the pathogen be intentionally or unintentionally introduced to the continental USA, there is tremendous potential for economic damages due to loss of livestock, trade restrictions, and subsequent food supply chain disruptions. We have incorporated the effects of space into a mathematical model of RVF in order to study the dynamics of the pathogen spread as affected by the movement of humans, livestock, and mosquitoes. The model accounts for the horizontal transmission of …

Information Fusion And Quantum Logic In Family Medicine, F. Matthew Mihelic

Faculty Publications

A time-tested model for information fusion and analysis is provided by Family Medicine. Family Medicine is a discipline that operates in the realms of botJ1 technical and social sciences to analyze massive amounts of information from mulriple sources in order to make decisions that bring about tJie integration and coordination of interdisciplinary interventions involving scientific, psychological, and social aspects of medical care. This generalist function serves to lower the organizational entropy of complex systems and thereby improve efficiency while reducing ilie risk of catastrophic failure. Simply bringing a group of specialists together in one place does not bring about the …

Sedation Safety Using Clinical Practice Guidelines, Bonnie Wasilowsky Bspa-Hca, Rn, Cnrn

Patient Care Services / Nursing

No abstract provided.

Infectious Intracranial Aneurysms: Collaboration For Treatment Success, Erin M. Conahan Rn, Bsn, Cnrn

Patient Care Services / Nursing

No abstract provided.

Gene Therapy In The Cornea: 2005--Present, Rajiv R. Mohan, Jonathan C. K. Tovey, Ajay Sharma, Ashish Tandon

Pharmacy Faculty Articles and Research

Successful restoration of vision in human patients with gene therapy affirmed its promise to cure ocular diseases and disorders. The efficacy of gene therapy is contingent upon vector and mode of therapeutic DNA introduction into targeted cells/tissues. The cornea is an ideal tissue for gene therapy due to its ease of access and relative immune-privilege. Considerable progress has been made in the field of corneal gene therapy in last 5 years. Several new gene transfer vectors, techniques and approaches have evolved. Although corneal gene therapy is still in its early stages of development, the potential of gene-based interventions to treat …

Vorinostat: A Potent Agent To Prevent And Treat Laser-Induced Corneal Haze, Ashish Tandon, Jonathan C. K. Tovey, Michael R. Waggoner, Ajay Sharma, John W. Cowden, Daniel J. Gibson, Yuanjing Liu, Gregory S. Schultz, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

PURPOSE—This study investigated the efficacy and safety of vorinostat, a deacetylase (HDAC) inhibitor, in the treatment of laser-induced corneal haze following photorefractive keratectomy (PRK) in rabbits in vivo and transforming growth factor beta 1 (TGFβ1) -induced corneal fibrosis in vitro.

METHODS—Corneal haze in rabbits was produced with −9.00 diopters (D) PRK. Fibrosis in cultured human and rabbit corneal fibroblasts was activated with TGFβ1. Vorinostat (25 μm) was topically applied once for 5 minutes on rabbit cornea immediately after PRK for in vivo studies. Vorinostat (0 to 25 μm) was given to human/rabbit corneal fibroblasts for 5 minutes or 48 …

Spontaneous Coronary Artery Dissection: A Rare But Deadly Cause Of Acs In Younger Patients, Daniel J. Makowski Do, David A. Cox Md

Department of Medicine

No abstract provided.

Malpositioned Pacemaker Lead Presenting As A Peculiar 12-Lead Ekg, Amit Nanavati Md, Robert F. Malacoff Md, Facc

Department of Medicine

No abstract provided.

Thomas Jefferson University, Department Of Pathology, Anatomy And Cell Biology, Oral Administration Of Apigenin Inhibits Metastasis Through Akt/P70s6k1/Mmp-9 Pathway In Orthotopic Ovarian Tumor Model., Jun He, Qing Xu, Min Wang, Chongyong Li, Xu Qian, Zhumei Shi, Ling-Zhi Liu, Bing-Hua Jiang

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Apigenin, a flavonoid commonly present in the daily diet, is known for its potential anti-tumor properties. However, the effect of apigenin via oral administration on tumor growth and metastasis remains unknown. In this study we developed an orthotopic ovarian tumor model in nude mice to test the effect of apigenin oral administration, and showed that apigenin inhibited the micrometastasis of cancer cells in the animal tumor model. To understand the mechanism of apigenin in inhibiting metastasis, we found that apigenin greatly inhibited MMP-9 expression and p-AKT and p-p70S6K1 levels in the tumor tissues compared to the control group. We further …