Digital Commons Network^™

Is Add-On Budesonide-Impregnated Nasal Dressing Useful Following Endoscopic Sinus Surgery With Perioperative Oral Steroid?, Onusa Taweewuthisub

Chulalongkorn University Theses and Dissertations (Chula ETD)

Some chronic rhinosinusitis with nasal polyps patients undergoing endoscopic sinus surgery (ESS) have unfavorable results despite proper postoperative treatments including oral and topical steroids. Steroid-impregnated absorbable nasal dressing has been shown to improve outcomes of the surgery. In some clinical practices, budesonide-impregnated nasal dressing is used together with perioperative oral steroid but the additional benefits of it are still unknown. This study aims to determine whether budesonide-impregnated nasal dressing had any benefits following ESS when a short course of oral steroid was given in perioperative period. This is a prospective, double-blinded, within person randomized, placebo-controlled study conducted in tertiary care …

Circulating-Tumor Dna And Cancer-Induced Gene Expression As Novel Liquid Biomarkers Of Liver Cancers, Pattapon Kunadirek

Chulalongkorn University Theses and Dissertations (Chula ETD)

Liver cancer is one of the most cancer-related mortality with high incidence worldwide. Hepatocellular carcinoma (HCC) is the most common liver cancer type. Currently, Liquid biopsy is known as a non-invasive biomarker tool that specifically represents tumor appearance, leading to improved patient outcomes. Therefore, the novel biomarkers of liquid biopsy in Thai HCC patients are still unexplored and needed for precision medicine. The main aims of this study were First, to explore genetic profiles from liquid biopsies of circulating cell-free DNA (cfDNA) and peripheral blood mononuclear cells (PBMCs) using next-generation sequencing (NGS) and identify novel biomarkers for HCC from these …

Genetic Diversity Of Merozoite Surface Protein 1 Gene Of Plasmodium Falciparum In Thailand, May Myat Thu

Chulalongkorn University Theses and Dissertations (Chula ETD)

In 2030, World health organization's target is to eliminate malaria at least in 35 countries. At present, Thailand is low risk of malaria so that, it has the potential to eliminate. About 10 years ago, malaria prevalence was high along the country border areas. To prevent the recurrence in those areas, evaluation of drug susceptibility of parasites and vaccine are important. Therefore, basic knowledge on genetic diversity in malaria parasite is needed. Merozoite surface protein 1(msp1), one of the vaccine candidate genes, is useful for monitoring genetic diversity of the parasite and the potential gene of vaccine. However, high diversity …

Pediatric Immunization Practices In Nephrotic Syndrome: An Assessment Of Provider And Parental Knowledge, Cheryl L Tran, David T Selewski, Gia J Oh, Jonathan P Troost, Susan F Massengill, Samhar I Al-Akash, Shefali Mahesh, Rasheda Amin, Isa F Ashoor, Rahul Chanchlani, Mahmoud Kallash, Robert P Woroniecki, Debbie S Gipson

Faculty, Staff and Student Publications

Background: Children with nephrotic syndrome (NS) are at high risk for vaccine-preventable infections due to the immunological effects from the disease and concurrent treatment with immunosuppressive medications. Immunizations in these patients may be deferred due to their immunosuppressive treatment which may increase the risk for vaccine-preventable infections. Immunization practices in children with NS continue to vary among pediatric nephrologists. This raises the question of whether children with NS are receiving the recommended vaccinations at appropriate times. Therefore, it is critical to understand the practices and patient education provided by physicians to patients on the topic of vaccinations.

Methods: After informed …

Exploring The Heteromeric Interface Of The 5-Ht2a-Mglu2 Receptor Complex, Mohamed Aarif Abdul Kareem

Theses and Dissertations

Schizophrenia is a serious mental disorder characteristic of several neurotransmitters including dopamine, serotonin, and glutamate being in imbalance. Early therapies focused solely on dopamine antagonism and second-generation antipsychotics focused on the dopamine and serotonin systems and their respective G protein coupled receptor (GPCR) proteins. Although debate for dimerization of certain classes of GPCR exist, the establishment of an mGlu2-5-HT_2A heterocomplex, which is implicated in schizophrenia is of interest. Previous studies have used a mutation-based approach to identify transmembrane domain 4 (TM4) as the domain responsible in mGlu2 for mediating heteromerization before narrowing down the individual amino acids responsible for …

The Quantified Elf, Umico Niwa

Theses and Dissertations

The Quantified Elf is an ongoing collection of scientific, medical, archaeological articles and excerpts from my personal journal. It is a way by which I explore the various ways Western notions of personhood subsume human life into constructs of sexuality and gender, overlooking the various other modes of unbridled existence: plant, microbial. fungal, animal, celestial bodies. The speculative medical papers propose novel forms of body modification to combat gender dysphoria.

An Identification Of Tgf-B'S Role In Ischemic Cholangiopathy, Courtney Chmielewski

Theses and Dissertations

Organ transplants are a necessary intervention for many diseases that result in end stage organ failure. The donation pool cannot match the demands of the transplant list, so expanding the pool to include donation after cardiac death (DCD) is desired. However, there are increased odds of graft failure and ischemic cholangiopathy leading to inferior outcomes when DCD livers are used. Ischemic cholangiopathy consists of multiple diffuse strictures and fibrosis of the bile ducts leading to a loss of epithelialization and fibrosis. The cellular mechanism is proposed to be epithelial-to-mesenchymal transition (EMT). TGF-�� is seen as a key initiator of EMT, …

Modeling Gardnerella Host-Pathogen Interaction: Characterization Of Vaginolysin And The Opacity Phenotype, Erin M. Garcia

Theses and Dissertations

Bacterial vaginosis (BV) describes a dysbiotic state of the vaginal mucosa, during which the dominance by beneficial lactobacilli species is lost, the vaginal pH increases, and the flora comprising the vaginal microbiome becomes more diverse. Bacterial taxa associated with this dysbiotic state interact to form a dense polymicrobial biofilm on the surface of the vaginal epithelium. This shift in the vaginal microflora is clinically important, even when symptoms are not noted, as women with BV are at increased risk for acquisition of other sexually transmitted infections, including HIV, and are more than two times as likely to experience preterm birth. …

Recovery From Visual Dysfunction Following Mild Traumatic Brain Injury Is Associated With Adaptive Reorganization Of Retinal Inputs To Lateral Geniculate Nucleus In The Mouse Model Utilizing Central Fluid Percussion Injury., Vishal C. Patel

Theses and Dissertations

Traumatic brain injury (TBI) is a leading cause of morbidity and mortality nationwide. Prevalence of mild TBI (mTBI) vastly outnumbers more severe forms however the associated morbidity has only recently gained public attention. Visual dysfunction is a significant component of mTBI associated morbidity with recovery of function linked with improvement in global outcomes. Examination of sensory and motor pathways in other brain injury paradigms support that recovery is largely dependent on adaptive plasticity of remaining connections. Current examinations of visual function recovery following mTBI is limited to identifying evidence for recovery and objective evidence for adaptive plasticity is limited. Therefore, …

Integration Of Multi-Omic Platforms To Understand The Regulation Of Genes In Human Disease, Eva Lancaster

Theses and Dissertations

Epigenetic mechanisms have been hypothesized to modify gene expression (GE) in response to both genetic variation and environmental exposures, ultimately contributing to the development of complex traits. Epigenome-wide association studies (EWAS) aim to test the theory that marks of DNA methylation (DNAm) have downstream consequences that result in the development of complex diseases. Although these methods have been successful in identifying DNAm patterns in complex traits, specific molecular mechanisms involved in the etiology of complex diseases remain unidentified. This knowledge gap does not originate from a lack of DNAm-trait associations, but rather stems from study design issues that affect the …

The Effect Of The Senolytic Abt-263 On Androgen Deprivation-Induced Senescent Prostate Tumor Cells, So Min Lee

Theses and Dissertations

Prostate cancer (PCa) is one of the leading causes of cancer-related deaths in men. Although standard treatments such as androgen deprivation therapies (ADT) and antiandrogens have increased survival for many patients, most men placed on these therapies will develop castration-resistant disease (CRPC). Previous studies have shown that these treatments have limited cytotoxicity and instead promote cell growth arrest. Our current work demonstrates that prostate tumor cells grown in the absence of androgens by using charcoal-stripped serum undergo senescence-mediated or senescent-like growth arrest, based on the cellular expression of senescence-associated-beta-galactosidase (SA-β-Gal). Our studies further suggest that this senescence is transient and …

Efficacy Of Mepolizumab Treatment In Oral Corticosteroid-Dependent Severe Eosinophilicasthma Patients With Chronic Rhinosinusitis With Nasal Polyps: Single Center, Real Life Study, İnsu Yilmaz, Murat Türk, Saki̇ne Nazi̇k Bahçeci̇oğlu, Nuri̇ Tutar, İnci̇ Gülmez

Turkish Journal of Medical Sciences

Background/aim: Oral corticosteroid (OCS)-dependent severe eosinophilic asthma with chronic rhinosinusitis with nasal polyps (SEACRSwNP) would be a suitable phenotype for mepolizumab treatment. This study evaluated the short-term efficacy of mepolizumab treatment in OCS-dependent SEA-CRSwNP. Materials and methods: Baseline and 24th week results [daily OCS doses, asthma exacerbation frequency, asthma control test (ACT) scores, blood eosinophil levels, FEV1 values, and numerical analog scale (NAS) of CRSwNP symptoms] of patients who were treated for at least 24 weeks with mepolizumab were retrospectively evaluated and compared. Results: A total of 16 patients were enrolled in the study. Mepolizumab was discontinued in one patient …

Tumour Budding In Preoperative Biopsy Specimens Is A Useful Prognostic Index Foridentifying High-Risk Patients In Early-Stage (Pn0) Colon Cancer, Mehmet Zengi̇n, Aydin Çi̇fci̇

Turkish Journal of Medical Sciences

Background/aim: Tumour budding (BD) is considered a valuable prognostic factor in colon cancer (CC), but its use in daily practice is uncertain. We investigated the prognostic effect of BD using preoperative biopsy specimens in a fairly homogeneous population. Materials and methods: Eighty-two (pN0) CC patients who underwent surgery after preoperative biopsy between 1997 and 2013 were included in the study. Model A (using the `deeply invasive blocks & hot-spot area & invasive margin) and method 1 (using the `20× objective & immunohistochemistry staining & quantitive counting') were used as standard methods. Results: High BD was significantly associated with poor prognostic …

Gynecological Laparoscopic Surgery In The Shade Of Covid-19 Pandemic, Şadiman Kiykaç Altinbaş, Ömer Lütfi̇ Tapisiz, Yaprak Üstün

Turkish Journal of Medical Sciences

A global public health problem with a high rate spread and transmission, Coronavirus outbreak has become the most talkedabout matter throughout the world. We are severely affected by the nations with vast numbers of deaths; it was hard to predict such a colossal pandemic with terrifying consequences. Elective surgeries are limited, but situations requiring an urgent gynaecological or obstetric surgical approach must still be performed during the COVID-19 pandemic. Concerns regarding surgical safety and the risk of viral transmission during surgery are of great importance. In this review, we aimed to summarize the concepts related to laparoscopic gynecological surgery during …

The Effects Of Blood Group Types On The Risk Of Covid-19 Infection And Its Clinical Outcome, Hakan Göker, Eli̇fcan Aladağ Karakulak, Haluk Demi̇roğlu, Çağlayan Merve Ayaz Ceylan, Yahya Büyükaşik, Ahmet Çağkan İnkaya, Salih Aksu, Ni̇lgün Sayinalp, İbrahi̇m Celaletti̇n Haznedaroğlu, Ömrüm Uzun, Murat Akova, Osman İlhami̇ Özcebe, Serhat Ünal

Turkish Journal of Medical Sciences

Background/aim: COVID-19 (Coronavirus disease of 2019) is an infectious disease outbreak later on declared as a pandemic, caused by the SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). It spreads very rapidly and can result in severe acute respiratory failure. The clinical studies have shown that advanced age and chronic diseases increase the risk of infection. However, influence of the blood groups on COVID-19 infection and its outcome remains to be confirmed. The aim of this study is to investigate whether there exists a relationship between the blood groups of the patients and risk of SARS-CoV-2 infection and the clinical outcomes in …

Clinical Characteristics And Long-Term Outcome Of Takayasu Arteritisin Iran: A Multicentre Study, Aida Malek Mahdavi, Nadereh Rashtchizadeh, Hadiseh Kavandi, Mehrzad Hajialilo, Sousan Kolahi, Mohammad-Reza Nakhjavani, Ali-Asghar Ebrahimi, Seyedmostafa Seyedmardani, Mansour Salesi, Mohsen Soroush, Alireza Khabbazi

Turkish Journal of Medical Sciences

Background/aim: This study aimed to evaluate the demographic, clinical, angiographic and prognostic characteristics of Takayasu arteritis (TA) in Iran. Materials and methods: A total of 75 patients with TA based on the American College of Rheumatology 1990 criteria for TA classification referred to the Rheumatology Centres, were followed-up from 1989 to 2019. Demographic, clinical, angiographic and prognostic characteristics were collected at baseline and last visit. Results: The mean age was 31.9 ± 9.8 years at the disease onset. Female to male ratio was 14. The median latency in diagnosis was 24 months. Pulse discrepancy in the arms, blood pressure discrepancy …

Should The Dose Of Levothyroxine Be Changed In Hypothyroidism Patients Fasting Duringramadan?, Arzu Or Koca, Murat Dağdevi̇ren, Mustafa Altay

Turkish Journal of Medical Sciences

Background/aim: Muslims worship by fasting from predawn (suhoor) until sunset (iftar) for 30 days in the religious month of Ramadan. In addition to prolonged hunger, patients fasting with a diagnosis of hypothyroidism take their doses of levothyroxine (LT4) outside of daytime fasting hours. The purpose of our study is to compare the values of hypothyroid patients which have been obtained through thyroid function tests before and after Ramadan. Materials and methods: Ninety-seven patients; ranging from 18 to 65 years old, who were followed with a diagnosis of hypothyroidism, who fasted during Ramadan, and who had no change of their LT4 …

The Effect Of Iatrogenic Subclinical Hyperthyroidism On Anxiety, Depression And Quality Of Life In Differentiated Thyroid Carcinoma, Nazli Gülsoy Kirnap, Özlem Turhan İyi̇di̇r, Yusuf Bozkuş, Şeri̇fe Mehli̇ka Işildak, Cüneyd Anil, Sevde Nur Firat, Canan Demi̇r, Asli Nar, Nesli̇han Başçil Tütüncü

Turkish Journal of Medical Sciences

Background/aim: Overt thyroidism is known to cause neuropsychiatric disorders but studies on subclinical hyperthyroidism (SCH) are limited. Subclinical hyperthyroidism induction by administering L-Thyroxine (LT4) is the standard treatment method in differentiated thyroid carcinoma (DTC) follow-up. Our aim was to investigate whether anxiety, depression and quality of life are affected in DTC patients followed-up with exogenous SCH. Materials and methods: The patients were divided into exogenous SCH by LT4-DTC (n = 127), euthyroid-DTC (n = 66) and exogenous euthyroid-benign thyroid noduüle (BTN) who underwent thyroidectomy for benign thyroid pathology (n = 85) groups. Results: The rate of moderate/severe anxiety was significantly …

The Assessment Of Skin Sebum And Moisture Content Of Infants With Atopic Dermatitis, Hati̇ce Güneş, Hülya Nazi̇k, Mehmet Yaşar Özkars, Nurgül Parlak, Ayten Yildiz, Ömer Duyuran, Bahaüddi̇n Ahmed Yalçin

Turkish Journal of Medical Sciences

Background/aim: Atopic dermatitis (AD) is a chronic, inflammatory skin disorder characterized by dry skin, pruritus and eczematous lesions. The aim of this study is to evaluate the skin moisture and sebum content of the infants with AD and its relationship between the disease severity. Materials and methods: For this cross-sectional and case-control study we included 49 infants with AD aged between 2 and 24 months and 34 healthy infants with the same age and sex as a control group. Skin moisture and sebum content were measured by the bio- impedance method and recorded. Skin prick tests, total IgE levels and …

The Association Between The Route Of Nutrition And Serum Levels Of Adipokines In Critical Illness: A Pilot Study, Kürşat Gündoğan, Ender Doğan, Nurhayat Tuğra Özer, Gülşah Güneş Şahi̇n, Serap Şahi̇n, Murat Sungur, İsmai̇l Hakki Akbudak, Sabahatti̇n Muhtaroğlu, Muhammet Güven, Thomas R. Ziegler

Turkish Journal of Medical Sciences

Background/aim: Adipokines play an important role in the regulation of metabolism. In critical illness, they alter serum levels and are suspected to worsen clinical outcomes. But the effect of the route of nutrition on adipokines is not known. The purpose of this study was to evaluate the association between the route of nutrition and adipokine levels in critically ill patients. Materials and methods: This prospective study was performed in an intensive care unit (ICU). Patients admitted to the ICU for least 72 h and receiving either enteral nutrition (EN) via tube feeding or parenteral nutrition (PN) were enrolled. Serum was …

The Effect Of Sacrospinous Ligament Fixation During Vaginal Hysterectomy On Postoperative De Novo Stress Incontinence Occurrence: A Prospective Study With 2-Year Follow-Up, Eralp Başer, Kerem Doğa Seçki̇n, Pinar Kadi̇roğullari, Hüseyi̇n Kiyak

Turkish Journal of Medical Sciences

Background/aim: To investigate the risk of de novo stress urinary incontinence (SUI) occurrence in women who were treated for pelvic organ prolapse (POP) with sacrospinous ligament fixation (SSLF) in addition to vaginal hysterectomy (VAH) and antero-posterior colporrhaphy (CAP) over a 24-month follow-up period. Materials and methods: A prospective randomized study was designed. Women without occult or obvious SUI were randomized into either one of the study groups: Group 1: VAH + CAP, and Group 2: VAH + CAP + SSLF. Postoperatively, the patients were reevaluated for de novo SUI occurrence. Results: A total of 150 women were analyzed [G1 = …

The Relationship Of The Immune Response Mediator Genes' Polymorphic Variants With The Methotrexate Efficacy In Juvenile Idiopathic Arthritis, Liliia Nazarova, Ksenia Danilko, Viktor Malievsky, Denis Karimov, Akhat Bakirov, Tatiana Viktorova

Turkish Journal of Medical Sciences

Background/aim: The aim of the study was to analyze the relationship of the immune response mediator genes' polymorphic loci (TNFA rs1800629, LTA rs909253, IL1B rs16944, IL2-IL21 rs6822844, IL2RA rs2104286, IL6 rs1800795, IL10 rs1800872, MIF rs755622, CTLA4 rs3087243, NFKB1 rs28362491, PTPN22 rs2476601, PADI4 rs2240336) variants with the methotrexate efficacy in juvenile idiopathic arthritis (JIA). Materials and methods: The study included 274 JIA patients from the Republic of Bashkortostan, Russia. Achieving the American College of Rheumatology Pediatric 30 (ACR Pedi 30) response was regarded as the presence of the response to methotrexate (otherwise, as the absence), while achieving clinical remission on medication …

Optimization Of Isothermal Amplification Method For Mycobacterium Tuberculosis Detection And Visualization Method For Fieldwork, Hati̇ce Esra Ağel, Hasan Sağcan, İsmai̇l Ceyhan, Riza Durmaz

Turkish Journal of Medical Sciences

Background/aim: Tuberculosis is still one of the most contagious diseases around the world. Key factors of tuberculosis control are rapid diagnostic, efficient treatment, and prevention of contamination by surveillance and monitoring. However, culture is the gold standard method for laboratory diagnosis of tuberculosis; the results are several weeks to obtain. In order to prevent contamination of tuberculosis, diagnosis must be made in short time and treatment should be started as soon as possible. The aim of this study is to optimize the loop-mediated isothermal amplification (LAMP) method, which provides a much faster and more sensitive result than the polymerase chain …

Does Capsaicin Have Therapeutic Benefits In Human Colon Adenocarcinoma? Selection Of The Most Reliable Dose Via Agnor, Mustafa Ni̇sari̇, Recep Eröz

Turkish Journal of Medical Sciences

Background/aim: To determine the effect of different doses of capsaicin on AgNOR protein synthesis in human colon adenocarcinoma derivate from colon cancer (Caco-2 cell). Materials and methods: In this experimental study, after the cultured of Caco-2 cell line, the cells are divided into 4 groups as control and different capsaicin exposed doses (25uµ, 50uµ, and 75uµ). Mean AgNOR number and total AgNOR area/nuclear area (TAA/NA) were calculated. Results: A significant differences were detected between control and capsaicin (50uµ) (P = 0.001), between control and capsaicin (75uµ) (P = 0.000), between capsaicin (25uµ) and capsaicin (50uµ) (P = 0.001) and between …

Immunomagnetic Separation And Listeria Monocytogenes Detection With Surface- Enhanced Raman Scattering, Hande Yeğenoğlu Akçinar, Belma Aslim, Hi̇lal Torul, Burcu Güven, Adem Zengi̇n, Zeki̇ye Suludere, İsmai̇l Hakki Boyaci, Uğur Tamer

Turkish Journal of Medical Sciences

Background/aim: We aimed to develop a rapid method to enumerate Listeria monocytogenes (L. monocytogenes) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods: Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also …

Nanotoxicity: A Challenge For Future Medicine, Ramazan Akçan, Hali̇t Canberk Aydogan, Mahmut Şeri̇f Yildirim, Burak Taşteki̇n, Necdet Sağlam

Turkish Journal of Medical Sciences

Background/aim: Due to nanomaterials' potential benefits for diagnosis and treatment, they are widely used in medical applications and personal care products. Interaction of nanomaterials, which are very small in size, with tissue, cell and microenvironment, can reveal harmful effects that cannot be created with chemically identical and larger counterparts in biological organisms. In this review, a challenge for future medicine, nanotoxicity of nanomaterials is discussed. Materials and methods: A detailed review of related literature was performed and evaluated as per medical applications of nanomaterials their toxicity. Results and conclusion: Most authors state "the only valid technology will be nanotechnology in …

Neuroprotective Effects Of Quercetin On Cerebral Vasospasm Following Experimental Subarachnoid Haemorrhage In Rats, Şanser Gül, Evren Aydoğmuş, Burak Bahadir, Mustafa Çağatay Büyükuysal, Berrak Güven

Turkish Journal of Medical Sciences

Background/aim: We examined the protective effects of the natural flavonoid, quercetin, against cerebral vasospasm in an experimental rat subarachnoid haemorrhage (SAH) model. Materials and methods: Thirty-eight albino Wistar rats were divided into five groups as follows: group 1 (G1, n=8), no experimental intervention; group 2 (G2, n=8), subarachnoid physiological saline; group 3 (G3, n=8), SAH; group 4 (G4, n=7) SAH and low-dose (10 mg/kg) quercetin treatment; group 5 (G5, n=7), SAH and high-dose (50 mg/kg) quercetin treatment. Subarachnoid haemorrhage was induced by injection of 0.15 cc of autologous blood taken from the tail artery into the cisterna magna from the …

Could Hif-1∝ Be A Novel Biomarker For The Clinical Course And Treatment Of Pulmonary Embolism?, Buğra Kerget, Dursun Erol Afşi̇n, Alperen Aksakal, Alperen Aksakal, Seda Aşkin, Ömer Araz

Turkish Journal of Medical Sciences

Background/aim: Pulmonary embolism (PE) is associated with high morbidity and mortality rates if not diagnosed and treated rapidly. The aim of our study was to investigate the relationship between levels of hypoxia-induced factor-1 alpha (HIF-1∝) and clinical course and prognosis in patients with intermediate low-risk, intermediate high-risk, and high-risk PE. Materials and methods: The study included 240 subjects in 4 groups: a healthy control group (n = 60, mean age = 60 ± 15.2, female/male = 30/30 ), intermediate low-risk PE group (n = 60, mean age = 60 ± 12,5, female/male = 27/33), intermediate high-risk PE group (n = …

Ocular Toxicity Of Intravitreal Golimumab In A Rabbit Model, Ceren Durmaz Engi̇n, Serap Ci̇laker Miçili, Osman Yilmaz, Hüsnü Alper Bağriyanik, Beki̇r Uğur Ergür, Fatoş Önen, Ali̇ Osman Saatci̇

Turkish Journal of Medical Sciences

Background/aim: To investigate the effect of intravitreal golimumab on rabbit retina histopathology. Materials and methods: Sixteen albino New Zealand rabbits were divided into three groups. The right eye of each rabbit in groups I, II, and III received a single intravitreal injection of 5 mg/0.05 mL (6 eyes), 10 mg/0.1 mL (6 eyes), or 20 mg/0.2 mL (4 eyes) golimumab, while left eyes served as controls with the same volume of a balanced salt solution injection. All animals were examined using slit-lamp biomicroscopy and indirect ophthalmoscopy before and after intravitreal injection and at days 1 and 7. Animals were euthanized …

Comparison Of Mesenchymal Stem Cell Sheets And Chondrocyte Sheets In A Rabbit Growth Plate Injury Model, Alper Gülteki̇n, Yücel Ağirdi̇l, Büşra Öncel Duman, Cansu Subaşi, Erdal Karaöz

Turkish Journal of Medical Sciences

Background/aim: The treatment of posttraumatic deformities and differences in length between the extremities resulting from physeal injury remains controversial. The aims of this study were to compare the efficacy of tissue-engineered, monolayer, and allogeneic mesenchymal stem cell sheets and chondrocyte sheets for physeal arrest treatment and to investigate cell sheet technology as a novel method for cell transplantation in physeal cartilage repair. Materials and methods: A proximal tibial physeal injury was induced in New Zealand rabbits. Allogeneic mesenchymal stem cells (MSCs) and chondrocytes were cultured in temperature-responsive culture dishes and applied to the iatrogenic partial growth plate defects in single-sheet …