Digital Commons Network^™

Effect Of Intramedullary Nailing Vs Plate Fixation On Bone Union Time In Distal Tibial Fractures, Madeline Wasmund

The PA Department Journal of Medical Science

Distal tibia fractures are complex fractures due to the intricate anatomy and minimal soft-tissue protection. There are currently two techniques that are mainstays for repairing a distal tibia fracture: intramedullary nailing and plate fixation. This study explores the bone union time between these two different techniques. PubMed was searched with a specific search strategy using the terms “intramedullary rod”, “plate/screw fixation”, “tibial fractures”, and “time to bone union.” Filters were applied to find articles that were most relevant, which resulted in 4 articles, each of which was appraised. Three articles were systematic reviews/meta-analyses comparing intramedullary nail fixation and plate fixation …

The Bidirectional Effects Of Traumatic Brain Injury And Caudal Fin Amputation On Regeneration In Zebrafish (Danio Rerio), Makenna Wiebe

Theses and Dissertations (Comprehensive)

Traumatic brain injury (TBI) and caudal fin amputation initiate robust regenerative responses in adult zebrafish (Danio rerio), yet whether regenerative activity in one tissue influences repair at a distant site remains unclear. This study investigated the bidirectional effects of concurrent brain and peripheral injury on regeneration and functional recovery. Specifically, we assessed the effects of TBI on caudal fin regeneration, caudal fin amputation on neural stem cell-associated cellular responses, and combined injury on behaviour. Adult zebrafish received no injury, TBI, caudal fin amputation, abdominal puncture, or combined TBI and caudal fin amputation. I assessed caudal fin regeneration morphometrically and evaluated …

Limosilactobacillus Reuteri Promotes Melatonin Release From Human Intestinal Organoids Via 5′Ectonucleotidase Activity, Heather A Danhof, Ludwig Ermann Lundberg, Stefan Roos, Robert A Britton

Faculty, Staff and Students Publications

Strains of Limosilactobacillus reuteri have been used to prevent or treat various conditions; however, the mechanisms by which they exert beneficial effects are not completely understood. Infant colic is one example in which L. reuteri DSM 17938 reduces clinical symptoms. While the etiology of colic is unknown, abnormal melatonin levels in infants have been suggested as a possible contributor. L. reuteri DSM 17938 has been shown to produce adenosine from AMP via production of the extracellular enzyme 5′ectonucleotidase (5′NT). Adenosine is a potent signaling molecule that impacts several important aspects of host physiology, including the release of melatonin from the …

Mucin-Derived Sugars Act As Metabolic Brakes Controlling Growth Initiation In Akkermansia Muciniphila, Bryan D Lakey, Katherine J Wozniak, Robert A Britton, Jeffrey J Tabor

Faculty, Staff and Students Publications

Akkermansia muciniphila is a key member of the gut microbiota and plays important roles in host metabolism and health. In the colon, A. muciniphila extracts nutrients from oligosaccharide-rich mucin glycans that comprise the mucosa. However, this environment is complex and shaped by dietary inputs, microbiome metabolism, and mucin glycan composition varying across hosts, gastrointestinal regions, and physiological states. How strains of A. muciniphila integrate these nutrient signals into growth initiation and niche colonization remains unclear. Here, we compare physiological responses of a human- and mouse-derived strain of A. muciniphila, finding that dietary sugars differentially affect these isolates, suggesting host-associated …

Gut-Derived Bacterial Extracellular Vesicles: The Microbial Dark Matter Contributing To Host Inflammation And Cardiometabolic Disease, Blayne Oliver, Michael Sun, Young Seo Lee, Ssanggoo Cho, Tianyi Zhu, David Durgan, Gary Sweeney

Faculty, Staff and Students Publications

Cardiometabolic diseases (CMDs) represent a growing global health burden, with chronic inflammation and gut microbiota dysbiosis emerging as central contributors to their pathogenesis. Among the diverse microbial products influencing host physiology, bacterial extracellular vesicles (BEVs) have gained attention as potent mediators of interkingdom communication. These nanoscale vesicles, secreted by both gram-positive and gram-negative bacteria, carry a complex cargo of proteins, lipids, nucleic acids, and pathogen-associated molecular patterns (PAMPs) that interact with host pattern recognition receptors (PRRs) to modulate immune and metabolic responses. BEVs can traverse intestinal barriers, disseminate systemically, and accumulate in cardiometabolic tissues, where they influence inflammation, insulin sensitivity, …

When Probiotics Guidelines Differ: A Practical Guide For Clinicians And Researchers, Howard Bauchner, Daniel J Merenstein

Faculty, Staff and Students Publications

Many professional organizations publish clinical practice guidelines for the use of probiotics in gastrointestinal disorders. Generally, no two guidelines align perfectly, and some differ markedly. These discrepancies occur because clinical practice guidelines are not purely mechanical outputs of data synthesis. They are shaped by structured interpretation, value judgments, and predefined thresholds for uncertainty. These elements become particularly visible when effect sizes are small, heterogeneity is high, and outcomes vary in clinical relevance-as is the case with probiotic research. This article examines why discordance among probiotic guidelines is predictable and provides practical guidance for clinicians who must decide among conflicting recommendations …

Steroid Receptor Coactivator 3-Deficient Regulatory T Cells Eradicate Multiple Solid Tumors In Syngeneic Mouse Models, Yuri Park, Adam M Dean, Yan Xia, Jianming Xu, Clifford C Dacso, David M Lonard, Sang Jun Han

Faculty, Staff and Students Publications

Steroid receptor coactivator 3 (SRC-3) is highly expressed in regulatory T cells (Tregs) and is important for their immunosuppressive activity. Recently, we demonstrated that disrupting SRC-3 expression in Tregs eliminates triple-negative breast cancer (TNBC) and prostate cancer in syngeneic animal models by generating an anti-tumor immune microenvironment without inducing immune-related adverse events (irAEs). Further analysis of these mice revealed that SRC-3 knockout (KO) Tregs infiltrated breast tumors and facilitated the infiltration of CD8

Is Your Cyst Really A Ganglion Cyst? A Case Report Of Angioleiomyoma Mimicking A Plantar Fascia Cystic Lesion, Christopher Anjorin Ms Dpmc, Joseph Bender Dpm

School of Podiatric Medicine - Student Research

Ganglion cysts are among the most common benign soft tissue masses encountered in podiatric clinical practice, and their diagnosis is often presumed based on location, clinical observation, and transillumination. However, this case highlights a diagnostic idiosyncrasy: a lesion clinically presumed to be a ganglion cyst within the plantar fascia was ultimately identified as an angioleiomyoma following surgical excision and histopathological evaluation. This finding underscores the importance of maintaining a broad differential diagnosis when assessing cystic lesions, particularly those that transilluminate, as benign vascular tumors may closely mimic more common entities in both clinical presentation and imaging. More importantly, what if …

Deep Brain Stimulation In Early-Stage Alzheimer's Disease: A Systematic Review Of Neuropsychological Outcomes, Nora Huey

Doctoral Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, memory impairment, and disruption of neural networks involved in cognitive functioning. Although existing treatments may provide symptomatic benefit, there remains a need for interventions capable of slowing or stabilizing disease progression. Deep brain stimulation (DBS) has emerged as a potential neuromodulatory intervention for early-stage AD through stimulation of memory- and cognition-related neural circuitry. This dissertation employed a systematic review research methodology to examine teh neuropsychological and neurobiological outcomes associated with DBS targeting the fornix (DBS-f) and nucleus basalis of Meynert (DBS-NBM) in individuals with early-stage AD over 12–24 …

Interplay Of Autophagy And Mtor Signaling On Amyloid-Beta, Samuel Wycoff

WUSM Theses and Dissertations – All Programs

Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β (Aβ), a process thought to result from an imbalance between Aβ production and clearance. Autophagy, a major intracellular degradation pathway regulated by the mechanistic target of rapamycin (mTOR), has emerged as a potential therapeutic target for restoring Aβ homeostasis, although its precise role in Aβ metabolism remains unclear. This study investigated how pharmacological modulation of autophagy and mTOR signaling influences Aβ levels in both in vitro and in vivo models.

Wild-type SH-SY5Y neuroblastoma cells were treated with a panel of autophagy-modulating compounds, and extracellular Aβ secretion was quantified alongside the …

Phytochemical And In-Vitro Cytotoxic Analyses Of Flavonoid Species Isolated From Chromolaena Leivensis, Luke A. Bryant

Electronic Theses and Dissertations

As a leading cause of death worldwide, cancer and its treatments remain a primary source of interest among researchers. Modern studies are looking into bioselective cytotoxicity to create new chemotherapies that do not have the same harsh side effects as those we use today. Natural product research has a promising future for the development of such treatments. Specifically, polar flavonoids may be the key to a bioselective treatment. Such flavonoid species are native to plants, often within flower petals or within the body. A natural antioxidant, they are designed not to harm healthy cells. This study focuses on the plant …

Genetic Drivers Of Progression In Alzheimer’S Disease Are Distinct From Disease Risk, Celeste E. Cohen, Shane Fernandez, Umran Yaman, Ahmad R. Ehyaei, Eleftheria Kodosaki, Aydan Askarova, Tenielle Porter, Eleanor O’Brien, Paul Maruff, Alexi Nott, John A. Hardy, Simon M. Laws, Dervis A. Salih, Maryam Shoai

Research outputs 2022 to 2026

Background: Recent trials in Alzheimer’s disease (AD) demonstrate encouraging outcomes. These trials target risk mechanisms identified through genetic analysis whilst directly aiming to reduce progression rates. Evidence from other neurodegenerative diseases suggests the genetics of progression is distinct from risk of disease. To expand these initial successes and improve clinical outcomes further we need to understand genetics of progression of disease. These can be deduced through rigorous analysis of meticulously phenotyped longitudinal cohorts. In this study we first looked at known genetic drivers of risk, namely polygenic risk scores for AD and APOE‑ε4, to assess their role in progression. This …

Comparative Performance Of Physiological Vital-Sign Forecasting Under Random And Patient-Wise Splitting Using Deep Learning, Lavanya Vasavi Chittem Reddy

Theses and Dissertations

Physiological vital-sign forecasting estimates future measurements based on recent temporal patterns and can support analysis of continuously recorded monitoring data. This study comparatively evaluated deep feedforward, recurrent, bidirectional recurrent, long short-term memory, and bidirectional long short-term memory architectures for one-step-ahead forecasting of peripheral oxygen saturation, heart rate, and pulse rate. Each model received consecutive observations of peripheral oxygen saturation, heart rate, pulse rate, respiratory rate, and age, while separate single-output models predicted the next value of the selected target.

Random and patient-wise data splitting were compared using identical input definitions, preprocessing procedures, model architectures, and training hyperparameters. The strongest architecture …

In-Hospital Mortality Patterns And Readmissions In Patients With Chronic Obstructive Pulmonary Disease: An Analysis Of The Role Of Pulmonary Hypertension, Saad Afzal Khan, Trishna Parikh, Adishwar Rao, Akriti Agrawal, Aarohi Parikh, Sarah Shin, Maulin Patel, Kha Dinh, Bela Patel, Bindu Akkanti

Faculty, Staff and Student Publications

Chronic obstructive pulmonary disease (COPD) may be complicated by pulmonary hypertension (PH). We aimed to understand the impact of PH on in-hospital mortality and quantify the 30-day readmission rate among patients with COPD. For this cross-sectional study, we used the Nationwide Readmissions Database from 2017-2020 to identify adults ≥18 years with COPD. Patients were stratified according to PH diagnosis. Baseline characteristics between groups were compared using the Pearson chi-square test and two-sample t-test. Predictors of in-hospital mortality were determined using multivariate logistic regression analysis adjusted for demographics and confounders. The 30-day readmission rate and prevalence of PH subgroups by baseline …

When Defense Turns Destructive: Unraveling The Global Rise Of Autoimmune Disease And Sex-Based Disparities, Avery R. Hammersmith

Caroliniana Undergraduate Research Journal

This article examines the evolution of autoimmunity from its early rejection under the theory of "horror autotoxicus" to its current status as a pressing global epidemic. While early 20th-century scientists believed the body possessed inherent safeguards against self-attack, mid-century discoveries of autoantibodies established that the immune system can indeed target healthy tissues, leading to chronic conditions such as systemic lupus erythematosus (SLE) and Hashimoto's thyroiditis. Current data reflect a significant surge in these disorders, with a reported 19.1% increase in incidence and a 12.5% increase in prevalence between 1985 and 2015. Within the United States, the prevalence of antinuclear antibodies, …

Forniceal Deep Brain Stimulation Improves Memory In Rett Syndrome Mice By Promoting The Maturation Of Newborn Hippocampal Neurons, Qi Wang, Bin Tang, Zhenyu Wu, Huda Y Zoghbi, Jianrong Tang

Faculty, Staff and Students Publications

Deep brain stimulation (DBS) is associated with an increase in hippocampal neurogenesis and memory benefits, but the causal mechanism is unclear. Here, we use a mouse model of Rett syndrome, a severe learning disability syndrome caused by MECP2 loss of function, to find that DBS promotes the maturation and integration of newborn dentate granule cells (DGCs) into the neural circuit. Suppressing hippocampal neurogenesis prevented DBS from improving learning and memory. Newborn methyl-CpG-binding protein 2 (MeCP2)-deficient DGCs in Rett syndrome mice showed attenuated development and synaptic dysfunction, but DBS rescued DGC dendritic arborization, synaptogenesis, and long-term synaptic plasticity. DBS achieves this, …

Dose-Dependent Pharmacodynamic Interaction Between Fasudil, A Rho Kinase Inhibitor, And Doxorubicin In The Mcf-7 Breast Cancer Cell Line, Ahmed B. Abdulwahid, Shaymaa F. Abbas, Dhuha E. Omran, Nehaya M. Al-Aubody, Sara A. Yaseen

Maaen Journal for Medical Sciences

Background: It is necessary to seek combination therapy that uses non-traditional cytotoxic drugs to mitigate the side effects of doxorubicin. Recently, Rho-associated protein kinase (ROCK) inhibitors have exhibited promising antitumor activity in both experimental and clinical studies

Aim: This study aimed to characterize the pharmacodynamic interaction between fasudil, a ROCK inhibitor, and doxorubicin in MCF-7 breast cancer cells by evaluating their cytotoxic effects and quantifying drug interactions using the Chou–Talalay principle.

Methods: Doxorubicin, fasudil, and their combined fractions were evaluated to investigate their cytotoxic impact on the MCF-7 cell line using the MTT  method. The cells were treated with …

Multiple Quality Control Checkpoints Safeguard Small Nuclear Rna Biogenesis And Prevent Assembly Of Aberrant Spliceosomes, Tiantai Ma, Claire Huntington, Zhuoyi Song, Rea M Lardelli, Eric L Van Nostrand, Jens Lykke-Andersen

Faculty, Staff and Students Publications

Defective small nuclear (sn)RNAs are produced from hundreds of human snRNA pseudogenes and mutant snRNA genes associated with human developmental disorders. Machineries that prevent defective snRNAs from disrupting pre-mRNA splicing remain poorly defined. Here, we identify multiple checkpoints in snRNA biogenesis monitored by quality control machineries that subject defective snRNAs to degradation and prevent their assembly into spliceosomes. We show that variant U1 snRNAs produced from human pseudogenes, some at rates approaching canonical snRNAs, are impaired in 3' cleavage and targeted for degradation by the NEXT-exosome while failures in subsequent protein assembly steps promote NEXT-exosome- or terminal uridylyl transferase 4/7-mediated …

Natural History Of Pi-Rads 3 Lesions On Serial Prostate Mpmri, Ron Marom, Yuval Rand, Meitar Atias, Sophie Barnes, Ibrahim Fahoum, Gal Keren-Paz, Mario Sofer, Ofer Yossepowitch, Snir Dekalo, Avi Beri, Roy Mano

Faculty, Staff and Students Publications

Objective: PI-RADS 3 lesions on prostate multiparametric MRI (mpMRI) are indeterminate findings with debated management. We evaluated their radiologic evolution on follow-up mpMRI and correlated imaging changes with biopsy outcomes.

Methods: We retrospectively identified 166 men with at least one PI-RADS 3 lesion on initial mpMRI and at least one follow-up mpMRI without intervening treatment. Lesions were classified at follow-up as regressed (downgraded to PI-RADS ≤ 2), stable (remained PI-RADS 3), or progressed (upgraded to PI-RADS 4-5). Lesions were stratified by zonal location. MRI-targeted biopsy results were reviewed when available (n = 64).

Results: On follow-up, 52% of lesions regressed, …

Implementation Of A Preventative Screen To Detect Heartworm Disease In Local Mosquito Populations, Ava Hurley, Emma Weissmeier, Lucy Corten, Jayden Ockert, Jackson Greenwold-Claiborne, Addison Levitz, Carson Mickey, Alexandra Bonner, Paul E. Richardson

Journal of the South Carolina Academy of Science

Heartworm disease, caused by the parasite Dirofilaria immitis, affects thousands of mammalian pets across the nation annually and is increasingly spreading. Various mammalian pets including dogs, cats, ferrets, and horses are affected by the disease, and currently dogs are the only mammalian pets with an approved treatment. This study aims to implement a preventative approach to combating the disease by utilizing a PCR-based screen to detect D. immitis in mosquito populations. Five key objectives were established in this study: refine a preventative screen to detect heartworm DNA in mosquitoes, implement the preventative screen through partnerships with local animal shelters, …

Development Of Genomic Detection Techniques Of Aquatic Pathogens To Aid In Disaster Relief After Hurricanes, Ana Rose Johnson-Redmon, Abigail F. Turka, Rylie Masterson, Paul E. Richardson

Journal of the South Carolina Academy of Science

Extreme weather events such as hurricanes, tropical storms, and flooding can introduce microbial contaminants into freshwater systems through stormwater runoff, animal waste, and damaged or overwhelmed wastewater infrastructure. Rapid detection of waterborne pathogens is important for environmental monitoring and public health response, especially in coastal regions vulnerable to hurricane-related flooding. This study developed and refined a polymerase chain reaction (PCR)-based environmental DNA detection technique to identify aquatic pathogens in freshwater systems. Water samples were collected from four freshwater sites, including three man-made ponds and one drainage ditch, during the Fall 2025 and Spring 2026 semesters. Target organisms include Giardia, Cyclospora, …

Investigating The Prevalence Of Human Papilloma Virus In A College-Aged Sample Group And Its Link To Sexual Behaviors And Attitudes, Gabriela Lewczyk, Eva Pollak, Phoebe Stryker, Samantha Hickey, Emily Moyer, Katelyn Ambrose, Alexander Knight, Rahjai Thomas, Adriana Burk, Paul E. Richardson

Journal of the South Carolina Academy of Science

Human Papilloma Virus (HPV) is the most common sexually transmitted infection (STI) in the United States, infecting up to 75% of sexually active individuals, with a prevalence rising significantly around age nineteen for oral and genital infections. Because the virus infects epithelial cells and is transmitted primarily through skin-to-skin contact, it is found mainly in the cervix, oral cavity, and on/within ones’ skin. While most of the 200+ HPV subtypes are benign and tend to self-clear within six months to two years, twelve of these strains are classified as high-risk oncogenic strains that drive malignant progression. Furthermore, HPV is linked …

Development Of A Molecular Screen For Community-Based Hookworm Detection, Emma Weissmeier, Ava Hurley, Jackson Greenwold-Claiborne, Morgan Deeringer, Paul E. Richardson

Journal of the South Carolina Academy of Science

Hookworm disease, caused by Ancylostoma duodenale and Necator americanus, is a soil-transmitted infection affecting over 700 million people worldwide. Transmission occurs through direct skin contact with contaminated soil, leading to health consequences including iron-deficiency anemia, malnutrition, and long-term developmental impacts. The CCU Disease Hunters program developed a PCR-based environmental screen to detect hookworm DNA in soil samples before transmission reaches human or animal hosts. Initiated in response to a request from All 4 Paws Animal Rescue, this project established protocols for soil collection, DNA extraction, and molecular identification of hookworm using the cytochrome c oxidase (COI) and cytochrome b (COB) …

Neuroinflammation As A Unifying Mechanism In Major Psychiatric Disorders: From Molecular Pathways To Clinical Implications: A Systematic Search And Review, Muath A Alammar

Neurosciences

Objective: To examine whether neuroinflammation can be understood as a unifying mechanism in major psychiatric disorders and summarized its molecular and clinical relevance. Major psychiatric disorders, including major depressive disorder (MDD), bipolar disorder (BD), and schizophrenia, are increasingly linked with immune dysregulation and inflammatory signaling. Neuroinflammation may help explain shared biological mechanisms across these disorders, but the available evidence remains diverse and clinically heterogeneous.
Methods: This review followed PRISMA 2020 guidance. Electronic searches were conducted in PubMed, ScienceDirect, Cochrane Library, PubMed Central, and Embase for studies published between 2021 and 2026. Only English-language, human studies published between 2021 and 2026 …

Evaluating The Role Of Visceral Adiposity In Neurological Disorders: A Systematic Review And Meta-Analysis, Layan A. Aldeaiji, Atheer G. Almutairi, Latifh M. Alharbi, Khalid M. Alsulaim, Emtenan E. Alshalahi, Yara K. Alghamdi, Norah A. Alhamdan

Neurosciences

Background: Neurological disorders represent a major global health challenge. According to recent Global Burden of Disease (GBD) estimates, Alzheimer's disease and other dementias, Parkinson's disease, multiple sclerosis, epilepsy, and headache disorders together account for nearly 3% of the worldwide disease burden. While visceral adipose tissue has traditionally been viewed as a metabolic concern, emerging evidence highlights visceral adiposity as an independent risk factor and potential contributor to the pathogenesis and progression of several neurological disorders.
Materials and methods: This systematic review and meta-analysis was conducted in accordance with PRISMA guidelines to evaluate the association between visceral adiposity and neurological disorders. …

Dengue: A Global And Emerging Health Challenge, Christopher Artigas

Lynchburg Journal of Medical Science

Dengue virus, a rapidly expanding tropical arbovirus with four distinct serotypes, poses a growing global public health threat—particularly in regions where healthcare systems are ill-equipped to diagnose, treat, and manage its severe manifestations. Transmitted primarily by Aedes mosquitoes, dengue typically manifests as an acute febrile illness characterized by severe myalgias ("bone-breaking fever"), arthralgias, gastrointestinal distress, and hemorrhagic tendencies. In severe cases, the infection can progress to life-threatening dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Because definitive antiviral therapeutics remain in development, supportive clinical care, with a critical focus on timely intravenous fluid resuscitation, is the cornerstone of management. …

Contraception Or Complication: A Review Of Depo-Provera’S Pharmacology And Public Health Implication, Phylicia Carpenter

Best Integrated Writing

This review article explores the link between progestogens and meningioma risk and outlines the biological mechanisms that may contribute to tumorigenesis. Two progestogens are emphasized through the paper: cyproterone acetate and medroxyprogesterone acetate (Depo-Provera). An important French study examining the relationship between progestogens use and meningioma risk is discussed, along with the health risks associated with long-term use. Additionally, this review advocates for increased routine prescriber-patient consultations and stronger drug regulation to educate and protect patients, particularly those experiencing socioeconomic disadvantages. It is important to note that the FDA recently updated the label for injectable medroxyprogesterone acetate in December 2025 …

Best Integrated Writing Fall 2026 - Complete Edition, Wright State University School Of Humanities And Cultural Studies, Kristie Mckiernan

Best Integrated Writing

Best Integrated Writing includes excellent student writing from Integrated Writing courses taught at Wright State University.

Progressive Remodeling Of Global Protein Interaction Networks In A Mouse Model Of Tauopathy, Noah Lampl, Morgan C Stephens, Zihan Wang, Joseph T Nigro, Alejandro Rondon Ortiz, Ryan Hekman, Anna Lourdes F Cruz, Rebecca Roberts, Lulu Jiang, Juan Botas, Andrew Emili, Benjamin Wolozin

Faculty, Staff and Students Publications

Neurodegenerative disease is marked not just by loss of proteins or cells, but by dynamic rewiring of macromolecular interaction networks that precede and drive pathology. Here, we present a temporally resolved, systems-scale map of multi-protein complex remodeling in a tauopathy model, integrating co-fractionation mass spectrometry, quantitative phosphoproteomics, and machine learning to decode phosphorylation-dependent shifts in protein interactomes across disease progression. This interactomic atlas serves as a resource to investigate functional assemblies, including MAPT-Dpysl2 and Cyfip1-actin, as well as ptk2b-Kars/Dars and ptk2b-Msn-Farsb-complexes, that modulate early disease phenotypes in vivo. Notably, it captures the composition, dynamics, and regulatory state of protein complexes …

Fgfr1/Klb-Mfg-E8 Maintains Microvascular Integrity And Adaptive Cardiac Remodeling, Sanskruti R Gare, Nasser H Alatawi, Xinyi Chen, Jiayan Zhang, Claire Ross, Oveena Fonseka, Yihua Han, Namrita Kaur, Andrea Ruiz-Velasco, Xiangjun Zhao, Yingjuan Liu, Riham R E Abouleisa, Susanne S Hille, Tao Wang, Sabu Abraham, Bernard D Keavney, Martin K Rutter, Elizabeth J Cartwright, Gareth Howell, Tamer M A Mohamed, Oliver J Müller, Wei Liu

Faculty, Staff and Students Publications

Background: Endothelial defects in the heart are a contributor to cardiac pathological remodeling and dysfunction, which can be influenced by cardiomyocytes. Here, we explore cardioprotective crosstalk between cardiomyocytes and endothelial cells under diabetic conditions.

Methods: Type 2 diabetes was induced in male and female mice using a high-fat, high-sucrose diet in conjunction with low-dose streptozotocin. Cardiomyocyte-specific deletion of FGFR1 (fibroblast growth factor receptor 1) or KLB (beta-Klotho) was achieved by crossing floxed mice with αMHC (alpha-myosin heavy chain)-Cre transgenic mice, while the treatment potential was assessed by recombinant adeno-associated virus 9 delivery systems or administration of recombinant protein. Various in …