Digital Commons Network^™

Preoperative Awareness Of Nasal And Facial Aesthetic Deformities Among Rhinoplasty Candidates, Ağah Yeni̇çeri̇, Nagi̇han Gülhan Yaşar, Burak Hazir, Meli̇h Çayönü

Turkish Journal of Medical Sciences

Background/aim: This study primarily aimed to evaluate the preoperative awareness of nasal and facial anatomical deformities among patients scheduled for primary rhinoplasty. The secondary objective was to assess changes in patients’ self-perception and aesthetic expectations regarding surgical outcomes following preoperative consultation with the surgeon.

Materials and methods: In this prospective cross-sectional study, 56 adult patients seeking primary aesthetic rhinoplasty at a tertiary care center were included. Each participant completed a 10-item anatomical evaluation form. An experienced rhinoplasty surgeon independently evaluated the same parameters. Additionally, participants completed a two-item visual analogue scale (VAS) questionnaire both before and after the preoperative consultation. …

Evaluation Of Prognostic Value Of Vascular Endothelial Growth Factor And Endocan In Non-Small Cell Lung Cancer, Mesut Meli̇h Özercan, Burcu Ancin, Yi̇ği̇t Yilmaz, Ahmet Müderri̇soğlu, Serkan Uysal, Ulaş Kumbasar, Zeynep Saribaş, Erkan Di̇kmen, Riza Doğan

Turkish Journal of Medical Sciences

Background/aim: No reliable, easily measurable biomarker currently exists for the screening or prognosis of lung cancer. The present study evaluates the potential utility of vascular endothelial growth factor (VEGF) and endocan, which can be readily measured in blood samples, as biomarkers for screening and prognostic assessment in lung cancer.

Materials and methods: Included in the study were 38 patients with early-stage non-small cell lung cancer (NSCLC) and 29 controls. All patients underwent surgical intervention and were monitored for 1 month postoperatively. Serum VEGF and endocan levels were measured preoperatively and postoperatively. Clinical characteristics, laboratory data, and histopathological findings were recorded …

Chemotherapy Tolerance And Continuity Are Prognostic For Overall Survival In Patients With Localized And Advanced Biliary Tract Cancer, Erman Akkuş, Pinar Kubi̇lay Tolunay, Eli̇f Berna Köksoy, Hati̇me Arzu Yaşar

Turkish Journal of Medical Sciences

Background/aim: Biliary tract cancers (BTC) are relatively rare and have a poor prognosis in both localized and metastatic settings. Clinical trials tend to include patients who can tolerate treatments; however, chemotherapy eligibility, patterns, and survival may differ in the real world. The present study provides a 5-year overview of chemotherapy eligibility, patterns, tolerance, and survival in patients with resected and advanced BTCs.

Materials and methods: Included in the study were patients with resectable or advanced BTC (excluding ampullary cancers) diagnosed between 2019 and 2024. The demographic/clinical characteristics, chemotherapy eligibility, patterns, and survival outcomes of the patients were evaluated.

Results: Of …

Predictive Impact Of Pi-Rads 3 Lesion Volume/Total Prostate Volume Ratio In Prostate Cancer Diagnosis In Biopsy-Naïve Patients, Emrah Özsoy, Musab Ali̇ Kutluhan, Emre Tokuç, Ridvan Kayar, Samet Demi̇r, Kaan Meri̇ç, Meti̇n İshak Öztürk

Turkish Journal of Medical Sciences

Background/aim: To assess the potential of the ratio between PI-RADS 3 lesion volume and total prostate volume as a predictive parameter for guiding the decision to perform a biopsy in patients presenting with PI-RADS 3 lesions on multiparametric prostate magnetic resonance imaging (mpMRI).

Materials and methods: A total of 749 patients who underwent mpMRI due to suspected prostate cancer between January 2014 and August 2023 were scanned. Based on predefined inclusion and exclusion criteria, 308 patients were included. Age, total prostate-specific antigen (PSA) value, prostate volume measured in mpMRI, mpMRI result, PI-RADS 3 lesion volume, and biopsy results were collected. …

Subjective Memory Complaints Among Older Adults In Türkiye: Prevalence And Contributing Factors, Mustafa Çeti̇n, Zehra Sarikaya Demi̇rbaş, Mehmet İlki̇n Naharci

Turkish Journal of Medical Sciences

Background/aim: Subjective memory complaints (SMCs), defined as self-perceived declines in memory performance, are common among older adults and may serve as early indicators of neurocognitive impairment. Despite their clinical relevance, no previous studies have examined the prevalence and associated factors of SMCs in Türkiye.

Materials and methods: A total of 500 community-dwelling older adults were included in this retrospective, cross-sectional, observational study; all underwent a comprehensive geriatric assessment at a tertiary-level outpatient clinic. Participants with an mini-mental state examination score ≥27, independence in instrumental activities of daily living, and no diagnosis of mild cognitive impairment were assessed for SMCs. Sociodemographic …

Diagnostic Yield Of Clinical Exome Sequencing In Patients With Suspected Hereditary Neuropathy, Can Ebru Beki̇rcan-Kurt, Doruk Arslan, Naz Güleray, Sevi̇m Erdem-Özdamar, Burcu Balci-Hayta, Ersi̇n Tan

Turkish Journal of Medical Sciences

Background/aim: Hereditary neuropathies exhibit significant genetic heterogeneities, often making molecular diagnosis challenging. Clinical Exome Sequencing (CES) allows the simultaneous evaluation of a wide range of candidate genes, and can be considered an efficient approach to identifying underlying genetic causes. The present study assesses the diagnostic utility of CES in patients with clinically suspected hereditary neuropathy.

Materials and methods: Included in the study were 21 patients with clinically suspected hereditary neuropathy who underwent CES. DNA samples were isolated from peripheral blood and subjected to CES on an Illumina NextSeq 2000 system, while a bioinformatics analysis and variant interpretation were performed using …

Serial D-Dimer Measurements Dynamically Predict Severity In Acute Biliary Pancreatitis: A Prospective Observational Study, Kemal Tekeşi̇n, Tolga Canbak, Ayli̇n Acar, Fati̇h Başak, Hasan Kumru, Olgun Erdem

Turkish Journal of Medical Sciences

Background/aim: Early risk stratification is required in cases of acute biliary pancreatitis (ABP). Traditional scoring systems such as Ranson’s criteria are complex and often delay treatment. D-dimer, a basic marker of systemic inflammation and coagulation, has shown promise as a prognostic tool. The present study investigates whether the predictive accuracy of serial serum D-dimer measurements is superior to that of the Ranson and BISAP scoring systems for moderate-to-severe ABP.

Materials and methods: Included in this single-center prospective observational study were 264 patients diagnosed with ABP between July 2022 and July 2025 whose collected data were analyzed. The participants were categorized …

Impact Of Patient Education Meetings On Disease Knowledge In Chronic Urticaria Patients, Nazan Beyhan, Hati̇ce Eylül Bozkurt Yilmaz, Asli Çi̇ftci̇, Ömür Aydin, Di̇lşad Mungan, Betül Ayşe Si̇n, Gülfem Eli̇f Çeli̇k, Murat Türk, İnsu Yilmaz, Sevi̇m Bavbek

Turkish Journal of Medical Sciences

Background/aim: Chronic urticaria (CU) is a manageable disease with symptoms of itching and visible lesions that can disrupt daily life and reduce quality of life. The severity of symptoms can vary, and the cause is often unknown. Concerns among patients as regards to treatment and prognosis highlight the need for disease education to enhance self-management and reduce anxiety. The present study examines the effect of disease education on the knowledge level of CU patients.

Materials and methods: Patients with CU who presented to the allergy clinics of two tertiary university hospitals were invited to inperson education sessions including slide presentations …

Evaluation Of The Efficacy Of Radiofrequency Ablation Therapy In Cases With Parathyroid Adenoma, Bahri̇ Evren, Abdulkadi̇r Bozbay, İsmai̇l Okan Yildirim, Ömercan Topaloğlu, İbrahi̇m Şahi̇n

Turkish Journal of Medical Sciences

Background/aim: Radiofrequency ablation of solitary parathyroid adenoma has been used to treat primary hyperparathyroidism (pHPT) in high-risk patients for parathyroidectomy. This study aimed to evaluate the clinical efficacy of radiofrequency ablation for treating pHPT in patients with parathyroid adenomas.

Materials and methods: The sample for this retrospective study comprised all consecutive patients with solitary parathyroid adenoma treated with radiofrequency ablation between 2013 and 2021. Patients’ baseline serum calcium and parathyroid hormone (PTH) values were obtained. The patients were followed up with serial biochemical measurements after the intervention, then at the first-week, first-month, third-month, sixth-month, and twelfth-month follow-ups. The study’s primary …

Validity And Reliability Of The Turkish Version Of The Topical Corticosteroid Phobia (Topicop) Scale, Esra Ağaoğlu, Hi̇lal Kaya Erdoğan, Hi̇lal Çavuş, Selma Meti̇ntaş

Turkish Journal of Medical Sciences

Background/aim: Concerns about the use of topical corticosteroids (TCSs), commonly referred to as corticophobia, are widespread among dermatology patients and often lead to nonadherence to TCS treatment. This study aimed to evaluate the validity and reliability of the Turkish version of the topical corticosteroid phobia (TOPICOP) scale.

Materials and methods: The TOPICOP scale comprises 12 items grouped into 2 dimensions: beliefs (6 items) and worries (6 items). For cultural adaptation, the scale was forward and backward translated. The final Turkish version was administered to 123 patients with chronic dermatoses who rated their perceptions of TCSs using a 4-point Likert scale. …

Irisin And Adropin Decrease In Preobese Or Obese Individuals With Nonalcoholic Fatty Liver Disease, Cuma Mertoğlu, Yaprak Sule Örek, Bülent Albayrak, Yusuf Kemal Arslan, Taha Abdulkadi̇r Çoban

Turkish Journal of Medical Sciences

Background/aim: Nonalcoholic fatty liver disease (NAFLD) is a metabolic disease that is commonly observed in individuals with obesity. The present study investigates the irisin and adropin levels of sera in preobese or obese adults with NAFLD.

Materials and methods: Included in the study were 89 patients who were categorized into four groups: Group 1: 25 normal-weight individuals without NAFLD (Control group); Group 2: 17 preobese or obese individuals without NAFLD; Group 3: 24 preobese or obese individuals with Grade 1 NAFLD; and Group 4: 23 preobese or obese individuals with Grade 2–3 NAFLD. The demographic details of all participants were …

Etiology And Outcomes Of Upper Extremity Venous Thrombosis: A Retrospective Cohort Study, Selçuk Coşkun, Pinar Köksal Coşkun, Ferhat İçme, Mehmet Ali̇ Ceyhan

Turkish Journal of Medical Sciences

Background/aim: Upper extremity venous thrombosis (UEVT), encompassing upper extremity deep vein thrombosis (UEDVT) and superficial vein thrombosis (UESVT), is increasingly recognized due to invasive procedures and advanced diagnostics. This study characterizes the 5-year outcomes and etiological factors of UEVT in a large cohort.

Materials and methods: We conducted a retrospective cohort study of 304 consecutive adult patients with acute UEVT (2019–2025) at a tertiary care center in Türkiye. Demographics, medical history, thrombosis characteristics, treatments, and outcomes—including thrombosis resolution, pulmonary embolism, and mortality—were analyzed. Statistical analyses were performed using chi-square tests, multivariate logistic regression, and random forest models.

Results: Among the …

Comparison Of Spatiotemporal And Arm Swing Characteristics Of Gait Between Patients With Copd And Healthy Controls, Hi̇daye Yamikan, Ni̇hat Özgören, Serdar Aritan, Oğuz Karcioğlu, Aynur Demi̇rel

Turkish Journal of Medical Sciences

Background/aim: Impairments in spatiotemporal gait characteristics have been observed in patients with chronic obstructive pulmonary disease (COPD). However, arm swing characteristics—a fundamental component of gait that play a key role in maintaining gait stability and energy efficiency—have been largely overlooked. This study aimed to investigate the spatiotemporal characteristics of gait, together with arm swing patterns, in patients with COPD.

Materials and methods: A total of 20 patients with COPD (aged 40–65 years) and 20 age- and sex-matched healthy controls (HC) participated in this cross-sectional study. Spatiotemporal gait characteristics and arm swing data were recorded using a motion capture system equipped …

Exploring Gender Differences In The Impact Of Sleep And Fatigue On Disease Severity In Rheumatoid Arthritis: A Moderated Mediation Model, Musa Salmanoğlu, Habi̇p Yilmaz

Turkish Journal of Medical Sciences

Background/aim: Rheumatoid arthritis (RA) is often accompanied by fatigue and sleep disturbances, which aggravate disease severity. Gender differences in these interrelationships remain insufficiently understood. This study aimed to examine whether fatigue mediates the association between sleep quality and disease severity in RA, and whether these pathways differ by gender.

Materials and methods: A single-center study was conducted with 68 RA patients (55.9% female). Disease severity was assessed using the Routine Assessment of Patient Index Data-3, fatigue using the Bristol Rheumatology Fatigue Multidimensional Questionnaire, and sleep quality using the Pittsburgh Sleep Quality Index (PSQI).

Results: Fatigue significantly mediated the relationship between …

Outcomes Of The First Adolescent-Focused Smoking Cessation Clinic In Türkiye On Tobacco Control, Demet Taş, Furkan Kalayci, Alkim Öden Akman

Turkish Journal of Medical Sciences

Background/aim: Adolescent smoking remains common, highlighting the need to control tobacco use and identify influencing factors. This study aims to evaluate the outcomes of an adolescent-focused smoking cessation clinic and the factors influencing them.

Materials and methods: The study was conducted with 262 adolescents, who, along with their parents, had direct access to the clinic. Data were drawn from patient records containing structured registration and follow-up documentation. The study investigated the effects of baseline cigarette consumption, sociodemographic characteristics, and nicotine patch use on smoking cessation rates at three months, six months, and one year.

Results: The three-month, six-month, and one-year …

The Impact Of Tizanidine, Thiocolchicoside, And Cyclobenzaprine On Vascular Function In Ovariectomized Rats, Sümeyye Nur Gürsoy, Tolga Esmerli̇gi̇l, Uğur Berkay İnce, Turhan Dost, Buket Demi̇rci̇

Turkish Journal of Medical Sciences

Background/aim: Menopause is associated with increased vascular risk. This study investigated the effects of 3 centrally acting muscle relaxants—tizanidine (TZ), thiocolchicoside (TCC), and cyclobenzaprine (CBZ)—on vascular reactivity in ovariectomized (OVX) rats as a model of menopause.

Materials and methods: Body weight, blood glucose, blood pressure, and heart rate were recorded, and the rats underwent oophorectomy surgery. Eight weeks after the operation, the rats were divided into 5 groups: sham operated rats, OVX rats, OVX rats treated with TZ (OVX + TZ), OVX rats treated with TCC (OVX + TCC), and OVX rats treated with CBZ (OVX + CBZ). All drug …

Natural Progression Of Meningeal Lymphatic Dysfunction In App/Ps1 Mice Creates A Critical Window For Alzheimer’S Disease Intervention, Zilong Shen, Xibin Zhou, Chunxiang Zhou

Turkish Journal of Medical Sciences

Background/aim: Meningeal lymphatic vessels (mLVs) facilitate the clearance of toxic metabolites like amyloid-beta (Aβ) from the central nervous system. Dysfunction in MLVs is implicated in Alzheimer’s disease (AD). However, current knowledge relies on exogenous intervention models that fail to capture spontaneous mLV decline during AD progression. In this study, we investigated the age-dependent correlation between mLV/deep cervical lymph node (dCLN) dysfunction and Aβ pathology in APP/PS1 mice under noninterventional conditions.

Materials and methods: APP/PS1 and wild-type (WT) mice at 3, 6, and 9 months of age were evaluated. Cognitive function was tested using the Morris water maze. mLV/dCLN drainage was …

Moltkia Coerulea Extracts Alleviate Caspase-3 Activity Via Reducing Oxidative Stress In Lps-Induced Neurotoxicity In Bv-2 Cells, Asli Can Ağca, Derya Altay, Hüdaverdi̇ Kul, Asli F. Ceylan, Betül Sever Yilmaz

Turkish Journal of Medical Sciences

Background/aim: Moltkia coerulea, a medicinal plant traditionally used for its neuroprotective properties, contains diverse phenolic compounds. However, its mechanisms of action in neuroinflammation remain unclear. We hypothesized that extracts of M. coerulea may protect microglial cells from lipopolysaccharide (LPS)-induced oxidative stress and apoptosis by modulating reactive oxygen species (ROS) and caspase-3 activity.

Materials and methods: Aqueous and methanol extracts (5–50 μg/mL) were tested in BV-2 microglial cells. Cell viability was assessed by MTT assay, while ROS generation and caspase-3 activity were measured using fluorescence-based kits. The effective concentration (10 μg/mL) was selected for mechanistic assays based on viability data and …

Assessing The Efficiency Of Methods To Teach Adolescents About Testicular Cancer And Testicular Self-Examination, Ayşe Gül Güven, Laden Jafari̇, Murat Doğuş Günel, Özlem Akbulut

Turkish Journal of Medical Sciences

Background/aim: Due to the increasing incidence of testicular cancer (TC), early testicular self-examination (TSE) in adolescent males is crucial for early detection and treatment. However, it is uncertain which method is best for teaching about TC and TSE in this age group. This study aimed to assess the effectiveness of formal (face to face with model) and informal (video presentation) approaches in male adolescents about TC and TSE.Material and methods: This intervention study was conducted in 2 high schools, and included a study group (n = 142) and a control group (n = 102). In the study group, a video …

Topical Lidocaine For Pain Control With Intrauterine Device Insertion: Does It Work?, Piper Gaudet, Jahnavi Ramakrishnan, Fariha Azam

Physician Assistant Capstones, 2020-current

Objective: To evaluate whether topical lidocaine improves the subjective perception of pain during the insertion of an intrauterine device (IUD) in women of childbearing age, compared to a placebo. Design: Mini-systematic literature review. Methods: An initial search of PubMed, CINAHL, and Scopus was conducted using the terms "pain control," "IUD," and "lidocaine," yielding 49 articles, of which 31 were screened and 26 excluded based on pre-designated inclusion criteria. After assessing five articles for eligibility, three met the criteria and were evaluated using Duke University's Clinical Appraisal Worksheet and the Oxford Centre for Evidence-Based Medicine table to determine their level of …

Comparing Transcatheter Edge-To-Edge Repair (Mitraclip) To Surgical Approaches For Secondary Mitral Regurgitation, Iain Slater, Jordan Maddra

Physician Assistant Capstones, 2020-current

Objective: To compare clinical outcomes of surgical mitral valve replacement (SMVR) and TEER with MitraClip therapy in patients with secondary mitral regurgitation.

Methods: We performed a review of the literature of primary research comparing clinical outcomes of surgical mitral valve replacement (SMVR) and TEER with MitraClip therapy in patients with secondary mitral regurgitation. A preliminary search of the medical database PubMed was conducted in October 2024 using the terms mitral valve, secondary mitral regurgitation, transcatheter edge-to-edge repair, surgical candidacy, and comparative outcomes. Limits included “published in the last 15 years, humans, English, randomized controlled trials, and cohort studies.”

Results: After …

Clinical Characteristics Of Graves’ Disease Following Covid-19 Infection Or Vaccination: A Multicenter Case-Control Study, Asena Gökçay Canpolat, Kemal Ağbaht, Ati̇lla Hali̇l Elhan, Mustafa Cesur, Zi̇ynet Alphan Uç, Seçki̇n Akçay, Hülya Iliksu Gözü, Mehmet Aşik, Hayri̇ Bostan, Beki̇r Ucan, Tugçe Şah Ünal, Merve Yilmaz, Ayşe Kubat Üzüm, Mehmet Çağri Ünal, Cuneyd Anil, Ümmü Mutlu, Nurcan İnce, Sevgül Faki, Güven Bari̇s Cansu, Mehmet Sercan Erturk, Ayten Oguz, Mustafa Aydemi̇r, Şefi̇ka Burçak Polat, Oya Topaloğlu, Reyhan Ersoy, Beki̇r Çakir, Ayşe Ok, Ersen Karakiliç, Muhi̇tti̇n Yalçin, Yusuf Kayhan, Kader Ugur, Di̇lek Yazici, Alper Gürlek, Tülay Omma, Emre Serdar Saygili, Adnan Batman, Banu Kara, Göknur Yorulmaz, Bahri Evren, Füsun Baloş Törüner, Bülent Okan Yildiz, Murat Erdogan Fai̇k, Faruk Alagol, Erman Çakal, Mustafa Şahi̇n

Turkish Journal of Medical Sciences

Background/aim: To describe Graves’ Disease (GD) associated with COVID-19 infection (COVID) or its vaccines (VAC) and to compare the clinical presentations, laboratory parameters, and short-term clinical course of the disease among different etiology groups (COVID, VAC, and GD control).

Materials and methods: Included in this multicenter matched case–control, retrospective cohort study were 239 patients with newly diagnosed (n = 196) or recurrent GD (n = 43) associated with COVID (n = 79) or VAC (n = 160). Each case was matched (1:1) with a control who had been diagnosed with GD prior to COVID.

Results: The median age of the …

Electrochemical Detection Of No And Ca2+ During Cold Atmospheric Plasma Treatment Of Acute Wounds: Sensor Selectivity And Stability In The Plasma-Bio-System, Jonathan E. Thomas, Kristina Pattison, Suneel Kumar, Gagana Karkada, Duncan Trosan, Aunic Goodin, Jason Rainone, Dnyaneshwari Rananavare, Vandana Miller, Francois Berthiaume, Katharina Stapelmann

Kimmel Cancer Center Faculty Papers

Cold atmospheric plasmas (CAP) are a versatile tool in medical applications like wound healing. Its therapeutic benefits are partially attributed to the generation of biologically active reactive oxygen and nitrogen species (RONS). Characterization of RONS, however, typically only occurs after treatment. Here we report the first real-time in situ detection of CAP-generated nitric oxide (NO), and the simultaneous detection of cellular calcium ions (Ca²⁺) release using electrochemical sensors during CAP treatment of murine wounds. In vivo, NO rose rapidly within the first minute of CAP treatment but accumulated less overall than in PBS, reflecting reactions with wound-bed targets. In situ …

Association Between Gonial Angle And Mandibular Residual Ridge Resorption In Implant-Overdenture Patients, Pl Ranganayakidevi S Palaniappan, Norsiah Yunus, Norliza Ibrahim, Siti Fauzza Ahmad, Anitha Krishnan

Journal of Dentistry Indonesia

Objectives: The gonial angle could be one of the contributing factors for the continuous bone resorption in edentulous persons. This study aimed to examine the association between the gonial angle and RRR in edentulous patients receiving mandibular implant-supported overdenture (ISOD) and to compare different gonial angle measuring techniques. Methods: Twenty-three individuals with opposing mandibular ISOD and maxillary full dentures were recruited. The proportional area index was used to calculate the posterior RRR. Orthopantomographs (OPGs) pre- and four years post-implant insertion were compared. Comparison of the gonial angle measurements was made on 5 patients using four measurement methods: (i) Two different …

Anexelekto (Axl)/Mer Inhibitor Tamnorzatinib In Patients With Relapsed/Refractory Acute Myeloid Leukaemia: Results From A Phase I (Monotherapy) And Phase Ii (Combination With Venetoclax) Clinical Study, Margaret T Kasner, Nigel Courtenay-Luck, Courtney Dinardo, Sean M Post, Praneeth Baratam, George N Magrath, Takaaki Nakamura, Akifumi Fujii, Sergio Prados, Naoki Honda, Mary Mcbride, Paula Edwards-Holmes, Robert Stuart

Faculty, Staff and Student Publications

Introduction: Relapsed/refractory (R/R) acute myeloid leukaemia (AML) is a life-threatening haematological malignancy without effective treatments. Anexelekto (Axl) and Mer receptor tyrosine kinases have emerged as important therapeutic targets in AML for their crucial role in survival of AML cells. Tamnorzatinib (ONO-7475) is a potent and highly selective inhibitor of Axl/Mer. We report first-in-human study of tamnorzatinib (NCT03176277) in patients with R/R AML.

Methods: Tamnorzatinib was administered as monotherapy (n = 20) to determine an appropriate biological dose of tamnorzatinib and then in combination (n = 22) with venetoclax to evaluate safety and clinical efficacy.

Results: Tamnorzatinib was safe …

Agpat2 Acts At The Crossroads Of Lipid Biosynthesis And Drp1-Mediated Er Morphogenesis, Yoshihiro Adachi, Mauricio Torres, Allen H Hunter, Woo Jung Cho, Meixia Pan, Xianlin Han, Guangwei Du, Ling Qi, Miho Iijima, Hiromi Sesaki

Faculty, Staff and Student Publications

The morphology of the endoplasmic reticulum (ER), characterized by central sheets and peripheral tubules, is controlled by membrane-shaping proteins. However, the role of lipids in ER morphogenesis remains elusive, despite the ER being the major site for lipid synthesis. Here, by examining the role of eighteen phosphatidic acid (PA)-generating enzymes in ER morphology, we identify lysophosphatidic acid acyltransferase 2 (AGPAT2) as a critical factor in mouse and human cells. AGPAT2 produces PA in the glycerophospholipid/triacylglycerol biosynthesis pathway, and its mutations cause congenital generalized lipodystrophy. We find that AGPAT2-generated PA drives ER tubulation through gene knockout, 3D structural analysis by FIB-SEM, …

Measurable Imaging-Based Changes In Enhancement Of Intrahepatic Cholangiocarcinoma After Radiotherapy Reflect Physical Mechanisms Of Response, Brian De, Prashant Dogra, Mohamed Zaid, Dalia Elganainy, Kevin Sun, Ahmed M Amer, Charles Wang, Michael K Rooney, Enoch Chang, Hyunseon C Kang, Zhihui Wang, Priya Bhosale, Bruno C Odisio, Timothy E Newhook, Ching-Wei D Tzeng, Hop S Tran Cao, Yun S Chun, Jean-Nicholas Vauthey, Sunyoung S Lee, Ahmed Kaseb, Kanwal Raghav, Milind Javle, Bruce D Minsky, Sonal S Noticewala, Emma B Holliday, Grace L Smith, Albert C Koong, Prajnan Das, Vittorio Cristini, Ethan B Ludmir, Eugene J Koay

Faculty, Staff and Student Publications

Escalated doses of radiotherapy associate with improved local control and overall survival (OS) in intrahepatic cholangiocarcinoma (iCCA), but personalization remains limited because conventional size-based CT criteria correlate poorly with outcomes. We hypothesized that quantitative enhancement measurements would better predict clinical outcomes and guide individualized RT optimization. In a retrospective cohort of 154 patients, we analyzed pre- and post-RT CT scans using quantitative European Association for Study of Liver (qEASL) to derive viable tumor volumes, comparing enhancement-based metrics with size-based RECIST and linking them to outcomes via survival and mathematical modeling. Change in enhancement volume was strongly associated with OS after …

Stromal Fibroblastic Mutant Trp53 Promotes Mammary Tumor Development Via Enhanced Secretion Of Paracrine Factors, Bin Liu, Shunbin Xiong, Abie Williams-Villalobo, Gilda Chau, Yuan Qi, Hehua Chen, Carenza Johnson, Dhruv Chachad, Joy Mcdaniel, Xiaoping Su, Adel K El-Naggar, Guillermina Lozano, Yun Zhang

Faculty, Staff and Student Publications

Mutations in the tumor suppressor gene TP53 have been identified in breast cancer-associated fibroblasts and are associated with poor patient prognosis. However, the functional impact of fibroblastic mutant p53 on breast cancer development remains unclear. To investigate this, we compared female mice harboring HER2-driven mammary tumors with a fibroblast-specific Trp53 mutation (NP) to those with wild-type fibroblastic Trp53 (N). NP mice exhibited significantly shorter median tumor-free survival than N mice. RNA sequencing of NP and N tumors and mammary glands revealed numerous differentially expressed genes (DEGs) between tumors and the corresponding glands in both genotypes. Notably, the NP tumors showed …

Stakeholder Criteria For Trust In Artificial Intelligence-Based Computer Perception Tools In Health Care: Qualitative Interview Study, Ansh Rai, Meghan E Hurley, John Herrington, Eric A Storch, Casey J Zampella, Julia Parrish-Morris, Anika Sonig, Gabriel Lázaro-Muñoz, Kristin Kostick-Quenet

Faculty, Staff and Students Publications

Background: Computer perception (CP) technologies hold significant promise for advancing precision mental health care systems, given their ability to leverage algorithmic analysis of continuous, passive sensing data from wearables and smartphones (eg, behavioral activity, geolocation, vocal features, and ambient environmental data) to infer clinically meaningful behavioral and physiological states. However, successful implementation critically depends on cultivating well-founded stakeholder trust.

Objective: This study aims to investigate, across adolescents, caregivers, clinicians, and developers, the contingencies under which CP technologies are deemed trustworthy in health care.

Methods: We conducted 80 semistructured interviews with a purposive sample of adolescents (n=20) diagnosed with autism, Tourette …

A Review Of Cutaneous Viral Infections And Their Potential Role In Neurologic Diseases, Valeria Duque-Clavijo, Hung Q Doan, Stephen K Tyring

Faculty, Staff and Student Publications

Background: Cutaneous viral infections, defined as viral pathogens that either primarily affect the skin (e.g., herpesviruses, enteroviruses) or frequently produce dermatologic manifestations despite systemic tropism (e.g., HIV, SARS-CoV-2), can trigger systemic inflammatory and neurotropic responses that extend their impact to the nervous system. A growing body of evidence suggests that viruses with dermatologic manifestations may play a significant role in the pathogenesis of neurologic disorders.

Summary: Although individual viruses have been studied in isolation, the skin-brain axis in viral infections remains incompletely characterized. This review synthesizes existing knowledge and highlights gaps in understanding the mechanisms linking cutaneous viral infections to …