Digital Commons Network^™

Long Acting Beta2-Adrenergic Receptor Agonists Reverse Diabetic Nephropathy And Liver Steatosis In Murine Models Of Diabetes And Are Associated With Protection In Retrospective Human Studies, Brennan Winkler

MUSC Theses and Dissertations

Diabetes Mellitus (DM) affects 13.9% of men and 14.3% of women worldwide, a total of approximately 828 million people. Patients with diabetes are at risk of several complications including diabetic nephropathy (DN) and metabolic-associated steatohepatitis (MASH). These diseases eventually lead to end stage kidney disease (ESKD) and liver failure requiring dialysis and organ transplantation. In addition, diabetes exacerbates disease severity of autosomal dominant polycystic kidney disease (ADPKD), the most common potentially lethal genetic disease to affect humans with a prevalence of 1 in 500-1000. Therefore, a therapeutic approach that can attenuate organ damage and restore function in patients with DM, …

Dark And Camouflaged Genomic Regions Remain Challenging In Chm13, Mark E. Wadsworth, Madeline L. Page, Bernardo Aguzzoli Heberle, Justin B. Miller, Cody J. Steely, Mark T. W. Ebbert

Sanders-Brown Center on Aging Faculty Publications

Comprehensive genomic analysis is essential for advancing our understanding of human genetics and disease. However, short-read sequencing technologies are inherently limited in their ability to resolve highly repetitive, structurally complex, and low-mappability genomic regions, previously coined as “dark” regions. Long-read sequencing technologies, such as PacBio and Oxford Nanopore Technologies (ONT), offer improved resolution of these regions, yet they are not perfect. With the advent of the new Telomere-to-Telomere (T2T) CHM13 reference genome, exploring its effect on dark regions is prudent. In this study, we systematically analyze dark regions across four human genome references—HG19, HG38 (with and without alternate contigs), and …

Strengthening Rural Health Care: A Scoping Review Of Nurse Recruitment And Retention Strategies, Kelley Arredondo, Laura Witte, Alexander Paterson-Roberts, Alexandra Caloudas, Matthew Vincenti, Bradley V Watts, Bronwyn Sutherland, Hilary Touchett

Library Staff Publications

Background: Despite nursing being the largest US health profession, only 16% of registered nurses (RNs) practice in rural areas, where health care access is limited. This significantly impacts rural residents, including over 2.7 million rural veterans receiving care at the Veterans Health Administration, leading to compromised health outcomes. Nurses are vital in primary care, improving coordination and management of chronic conditions.

Objectives: Identify recruitment and retention programs for rural nurses (licensed vocational nurses/licensed practical nurses and RNs), including strategy characteristics and location.

Research design: We conducted a scoping review of published and gray literature. Eligible studies targeted US rural nurse …

Data-Driven Thresholds For Standardized Classification Of Severe Alzheimer’S Disease Neuropathology Using Digital Neuropathology, Ryan K. Shahidehpour, Allison M. Neltner, Mitchell A. Klusty, Cole Corbett, Angelique D. Gonzalez, David A. Gutman, David W. Fardo, Adam D. Bachstetter, Cody Bumgardner, Margaret E. Flanagan, Peter T. Nelson

Sanders-Brown Center on Aging Faculty Publications

Alzheimer's disease neuropathological changes (ADNC)—operationalized with semi-quantitative parameters—represent the consensus-based gold standard for diagnostic evaluation of disease severity. Although useful, ADNC diagnostic frameworks have limitations, particularly in advanced disease stages where pathological severity varies widely within a given diagnostic category. Further, some individuals lacking cognitive impairment are inappropriately categorized as having severe ADNC. In this study, quantitative pathology metrics and alternative tissue sampling schemes were integrated with data about premortem cognitive status, in order to derive clinically informed neuropathologic diagnostic thresholds. Specific goals of the current study were to generate data-driven, standardized diagnostic cut-points, with the most severe stages of …

Clonal Expansion Of Cytotoxic Cd8⁺ T Cells In Lecanemab-Associated Aria, Lance A. Johnson, Kai Saito, Akhil V. Pallerla, Jessica L. Funnell, Ashley R. Ezzo, Chelsea M. Song, Douglas A. Harrison, Noah J. Norton, Lauren C. Moore, Linda J. Van Eldik, David W. Fardo, Greg E. Cooper, Josh M. Morganti

Sanders-Brown Center on Aging Faculty Publications

Amyloid-related imaging abnormalities (ARIA) are the principal safety concern limiting anti-amyloid therapies for Alzheimer’s disease, yet their biology remains unclear. Here we show, through multi-omic profiling of peripheral blood from three ARIA+ patients and matched controls, that ARIA is associated with coordinated reprogramming of CD8 + T cells. CD8+ effector memory (TEM) and terminally differentiated (TEMRA) subsets were expanded, clonally enriched, and transcriptionally primed for cytotoxicity and vascular trafficking. Transcription factor inference and metabolomics converged on glycolytic reprogramming favoring short-lived effector function. Ligand-receptor modeling revealed enhanced monocyte-to-T cell signaling through antigen presentation, adhesion, and chemokine axes, while integration with a …

Reverse Koebner Phenomenon In Linear Scalp Morphea Following Punch Biopsy: A Rare Therapeutic Response, Suhas C. Gowda, Akshay Samagani, Shreyas C. Gowda

Digital Journal of Clinical Medicine

Morphea is a chronic immune-mediated fibrosing disorder characterized by excessive collagen deposition in the skin. Linear morphea of the scalp (en coup de sabre) commonly demonstrates the Koebner phenomenon, wherein lesions develop at sites of trauma; however, the reverse Koebner phenomenon—trauma-induced regression—is exceptionally rare. We report an unusual case of linear scalp morphea showing early regression following a diagnostic punch biopsy. A 71-year-old male presented with an asymptomatic, indurated linear plaque on the scalp of two months’ duration. Histopathology from a 3.5-mm punch biopsy confirmed morphea, demonstrating dense collagen bundles with lymphoplasmacytic infiltrate. At follow-up eight days after …

Corticotropin-Releasing Factor Neurons In The Bed Nucleus Of The Stria Terminalis Modulate Avoidance Behaviors And Feeding, Juan Manuel Romero, Shae-Marie Stafford-Trujillo, Mitzy Mendoza, Pey-Shyuan Chin, Snigdha Srivastava, Mikhail Kochukov, Qingchun Tong, Benjamin Russell Arenkiel

The Brown Foundation: Institute of Molecular Medicine

Introduction: Animals rely on defensive avoidance of exposed or potentially threatening environments as a fundamental survival strategy, often expressing this behavior as thigmotaxis, or a preference for the periphery of an open space. However, researchers still do not understand how neural circuits coordinate avoidance behavior with competing physiological drives such as feeding. In this study, we investigate how corticotropin-releasing factor (CRF)-expressing neurons in the bed nucleus of the stria terminalis (BNST) integrate environmental avoidance and feeding behavior.

Methods: We used adult male and female mice and applied fiber photometry to monitor BNST CRF neuronal activity in vivo. We performed both …

Mitochondrial Transfer To Granulocytic Myeloid-Derived Suppressor Cells Augments Immunosuppressive Activity, Prabhakar Arumugam, Cortney E. Heim, Rachel W. Fallet, Dhananjay Shinde, Vinai Chittezham Thomas, Rafael J. Argüello, Tammy Kielian

Journal Articles: Pathology and Microbiology

The anti-inflammatory properties of granulocytic myeloid-derived suppressor cells (G-MDSCs) promote Staphylococcus aureus (S. aureus) biofilm persistence. Evidence suggests that G-MDSC activity is shaped not only by S. aureus products but also by intrinsic metabolic programs. This study explores whether G-MDSC activity can be modulated by increasing mitochondrial abundance using a co-culture paradigm with macrophages as a mitochondrial donor. Macrophages transfer mitochondria directly to G-MDSCs via tunneling nanotubes, enhancing G-MDSC respiration, as reflected by increased basal, maximal, and spare respiratory capacity. Augmenting mitochondrial abundance in G-MDSCs enhances T cell-suppressive activity and reduces tumor necrosis factor (TNF) and interleukin 6 (IL-6) production. …

The Effects Of Antidepressant Medications On Sleep Quality In Dominican University Of California Students, Natalie L. Davis, Kenna Wohlford, Stephanie Lee

Physician Assistant Studies | Graduate Research Papers

We spend approximately one third of our life time sleeping or attempting to sleep. The quality of our sleep is equally, if not more important than the amount of sleep we get. This study assesses the effects that medications, specifically antidepressants, can have on sleep quality in Dominican University of California students. 118 participants completed a Qualtrics survey including questions regarding stress, sleep quality, medication lists, and demographics. This study utilized the Pittsburgh Sleep Quality Index (PSQI) to quantify participants’ sleep quality as the outcome. Participant stress levels were assessed with a Likert scale question asking for stress levels on …

From Fog To Function: An Occupational Therapist's Guide To Evaluating And Treating Cancer-Related Cognitive Impairments, Lauren E. Toner

Entry-Level Occupational Therapy Doctorate - Doctoral Capstone Symposium

Cancer – related cognitive impairment (CRCI) is a common side effect of cancer treatment that interferes with cancer survivors’ occupational performance, independence, and quality of life. Despite its high prevalence, CRCI remains under recognized and inconsistently addressed within oncology care. About 70% of individuals diagnosed with cancer report cognitive changes during or after treatment, yet many do not receive treatment. Occupational therapists are clinically trained to evaluate and treat functional cognition, activities of daily activities, instrumental activities of daily living, and client centered goals, positioning them as key providers in addressing CRCI. However, limited occupational therapy specific resources have hindered …

Hepatocyte Peroxisome Biogenesis And Phytol-Induced Metabolic Stress In Liver Disease, William Galloway

Theses, Dissertations and Capstones

Hepatocyte peroxisomes are required for metabolism of phytol-derived branched-chain fatty acids through the ACOX2-SCPx beta-oxidation pathway. This study tests whether hepatocyte-specific deletion of PEX16 (Pex16^Alb-Cre), which eliminates peroxisome biogenesis, sensitizes the liver to phytol-induced injury. The Pex16^Alb-Cre mice and their normal control Pex16^fl/fl mice were fed the Lieber-DeCarli liquid with or without 0.1 percent phytol for eight weeks. Serum alanine aminotransferase (ALT) and liver index were measured. Liver sections were stained with hematoxylin and eosin and Masson’s trichrome. Immunoblot analysis of peroxisomal enzyme expression was also performed. Phytol had no effect in Pex16^fl/fl mice as …

Sexually Dimorphic Effects Of Gpnmb Modulation On Vertebral Bone Mass Regulation And Intravertebral Disc Health, Hakem Altawil, Fayez Safadi

Williams Honors College, Honors Research Projects

Osteoporosis and vertebral degeneration are major contributors to musculoskeletal morbidity, yet the molecular mechanisms regulating vertebral bone and intervertebral disc (IVD) homeostasis remain incompletely understood. GPNMB (osteoactivin) has been identified as a regulator of bone remodeling, though its role in spine health is unclear. This study evaluated the effects of GPNMB deficiency on lumbar vertebral bone microarchitecture and IVD integrity in a sex-dependent context.

Six-month-old male and female wild-type (WT) and GPNMB knockout (KO) mice were analyzed using micro–computed tomography (µCT) of lumbar vertebrae (L4–L6) and histological assessment of the L5–L6 IVD. KO males exhibited increased bone volume fraction, bone …

A Hybrid Response Surface Methodology And Machine Learning Framework For Quantifying Effects Of Physicochemical Parameters On Pfas Distribution, Harsh V. Patel, Jazmin Green, Hyoshin Park, Stephanie Luster-Teasley Pass, Renzun Zhao

Engineering Management & Systems Engineering Faculty Publications

Predicting PFAS adsorption across diverse adsorbents and environmental matrices remains challenging because adsorbent physicochemical properties, PFAS molecular descriptors, and operational conditions simultaneously influence adsorption. This study develops and evaluates a unified hybrid modeling framework that integrates Response Surface Model (RSM) with machine-learning algorithms to quantify how six key variables, surface area, Log K_ow, pH_pzc, pK_a, log dose, and log-initial concentration, affect PFAS distribution coefficients (Log K_d). A data set of more than 1000 adsorption observations spanning 15 PFAS compounds, multiple adsorbent types, and a broad operational range was compiled and preprocessed using …

Metabolic Syndrome Beyond Diagnostic Criteria: Population-Scale Integrative Metabolomics Characterization, Marwa Talal

Theses and Dissertations

Background: Metabolic syndrome (MetS) is a complex cluster of interrelated metabolic abnormalities associated with elevated cardiometabolic risk. While diagnosis is based on well-established five clinical criteria, these may overlook early or atypical metabolic alterations. Large-scale metabolomic profiling offers an opportunity to identify biochemical signatures of MetS beyond diagnostic bias and to evaluate their relative importance across different presentations of the syndrome.

Methods: Data from 117,147 UK Biobank participants were analyzed in a cross-sectional design. High-throughput NMR quantified 75 circulating metabolites, for. Univariate analyses, MetS subtype stratification, and elastic net models with SHAP interpretation were applied to assess feature …

Reply To: S. N. Katkuri Et Al. And H. Liu Et Al. On Early And Sustained Improvements In Sense Of Smell With Tezepelumab Treatment In Patients With Chronic Rhinosinusitis With Nasal Polyps (Waypoint), Joaquim Mullol, Joseph K. Han, Tanya M. Laidlaw, Claire Hopkins, Anju T. Peters, Oliver Pfaar, Martin Desrosiers, Stella E. Lee, Andrew P. Lane, Claudia Chen, Yun Chon, Sandhia S. Ponnarambil, Andrew Foster, Andrew W. Lindsley, Christopher S. Ambrose

Department of Otolaryngology (ENT) Faculty Publications

[Introduction] We thank Dr. S. N. Katkuri, Dr. H. Liu, and their coauthors [1, 2] for their interest in our recent publication describing the improvements in loss of smell symptoms with tezepelumab versus placebo in patients with uncontrolled chronic rhinosinusitis with nasal polyps (CRSwNP) in the WAYPOINT trial (NCT04851964) [3]. We are grateful for the authors' feedback on the clinical significance of the data presented and their appreciation of the consistency observed across a range of baseline clinical characteristic and demographic subgroups.

Single Intratympanic Injection Of Proprietary Brain-Derived Neurotrophic Factor (Oto-413) In Subjects With Speech-In-Noise Hearing Impairment: A Randomized, Dose Escalating, Double-Blind, Placebo-Controlled Phase 1/2 Study, Alan C. Foster, Jeffery J. Anderson, David R. Moore, Morgan Oktela Fuentes, Yiwei Wang, Peter G. Volsky, K. Paul Boyev, Victoria A. Sanchez

Department of Otolaryngology (ENT) Faculty Publications

Purpose

To evaluate the safety and tolerability and exploratory efficacy of OTO-413, an intratympanic-administered, sustained release formulation of brain-derived neurotrophic factor (BDNF) in a thermoreversible gel, in participants with speech-in-noise hearing difficulties.

Methods

This was a single dose, dose-ascending, randomized, double-blind, Placebo-controlled Phase 1/2 study conducted at 7 enrolling clinical sites in the U.S. 110 participants (56% female) enrolled had self-reported speech-in-noise (SIN) difficulties that were confirmed by a SIN test and were randomized to OTO-413 or Placebo. OTO-413 dose range was escalated from 0.01 mg to 1.5 mg within 7 Cohorts. Safety evaluations included monitoring for treatment-emergent adverse events, …

Comparison Of Fully Covered Self-Expandable Metal Biliary Stents With Or Without Anchoring Double-Pigtail Plastic Stents: A Systematic Review And Meta-Analysis, Samuel Tanner, Fadi Hawa, Alejandra Vargas, Priyata Dutta, Antonio Facciorusso, Jean M. Chalhoub, Jorge D. Machicado

Department of Medicine Faculty Publications

Background and AIMS

Fully covered self-expandable metal stents (FcSEMSs) have become the mainstay of treatment for a variety of biliary pathologies. However, FcSEMSs have been associated with a greater rate of stent migration than other types of stents. A technique of anchoring double-pigtail plastic stents (DPPSs) has been proposed to minimize migration of biliary FcSEMSs. Herein, we evaluated the efficacy of anchoring DPPSs among patients treated with biliary FcSEMSs.

Methods

We performed a systematic review using PubMed, EMBASE, and Web of Science from database inception through August 2023. Full-text articles comparing FcSEMSs with or without anchoring DPPSs were included. We …

The Role Of Mscs In The Il23/17 Pathway And Their Response To Mechanical Stress, Norma Aileen Dominguez Jimenez

Honors Theses

Mechanical stress plays a central role in shaping cellular behavior within skeletal tissues. At the enthesis, where tendons and ligaments attach to bone, repeated mechanical loading is strongly associated with inflammation and the development of ankylosing spondylitis (AS). Although mesenchymal stem cells (MSCs) are known to contribute to tissue structure and repair at these sites, their potential role in inflammatory signaling, particularly within the I 23/IL 17 pathway, remains unclear. In this thesis I investigated whether MSCs are capable of expressing cytokines involved in this pathway and how mechanical stress and differentiation influence this expression. Using the mouse derived C3H/10T1/2 …

Unlocking The Power Of Cxcr2 Inhibition To Overcome Gemcitabine Resistance In Pancreatic Cancer, Caitlin Molczyk, Reegan Sturgeon, Sugandha Saxena, Esther Johnson, Rakesh Bhatia, Namita Bhyravbhatla, Sushil Kumar, Surinder K. Batra, Rakesh K. Singh

Journal Articles: Biochemistry & Molecular Biology

Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer-related mortality, characterized by intrinsic resistance to conventional therapies and limited effective treatment options. In this study, we investigated the role of the CXCR2 axis in PDAC therapy resistance. CXCR2, a chemokine receptor, is actively involved in inflammation, tumor angiogenesis, and metastasis. Our working hypothesis is that CXCR2 contributes to PDAC chemotherapy resistance. To test this, we generated gemcitabine-resistant (GemR) lines using T3M4 and CD18/HPAF (CD18) cell lines. Baseline expression of CXCL1, CXCL5, and CXCL8 ligands was higher in GemR cells compared to parental cells. Upon gemcitabine treatment, parental cells …

Midkine (Mdk) As A Central Regulator Of The Tumor Microenvironment: From Developmental Cytokine To Therapeutic Target, Hareesh B. Nair, Ajay Nair, Ya-Guang Liu, Dileep K. Vijayan, Ramadevi Subramani, Rajkumar Lakshmanaswamy, Suryavathi Viswanadhapalli, Gangadhara R. Sareddy, Surinder K. Batra, Ratna K. Vadlamudi

Journal Articles: Biochemistry & Molecular Biology

Midkine (MDK) is an oncofetal, heparin-binding cytokine that is re-expressed across diverse cancers and correlates with aggressive disease and treatment resistance. This review synthesizes current evidence on MDK as a coordinator of tumor-intrinsic signaling and microenvironmental remodeling. We summarize MDK structural features, extracellular matrix interactions, and receptor systems that mediate MDK signaling, highlighting LRP1 and PTPRZ1 with context-dependent participation of ALK, nucleolin and integrins. Downstream, MDK engages MAPK, PI3K-AKT, STAT3 and NF-κB pathways to promote tumor cell survival, epithelial-mesenchymal plasticity, and therapeutic stress tolerance. We then focus on tumor microenvironment (TME) programs shaped by MDK, including angiogenesis, fibroblast activation and …

Maxillary Anterior Teeth Replacement With Immediate Dental Implants Utilizing Vestibular Socket Therapy Versus Ice Cream Cone Technique, Mohamed Faisal Abd El Raorf Shoeib, Mohammed Abdel-Monem Tawfik, Mohammed Talaat Salem, Ayman Ahmed Mustafa Yaseen

Mansoura Journal of Dentistry

Objective: This study intended to evaluate changes, clinically and radiographically, associated with the immediate placement of dental implants in the maxillary anterior esthetic zone, comparing vestibular socket therapy with the ice cream cone technique. Materials and Methods: The current research included sixteen individuals requiring the replacement of a non-restorable tooth in the maxillary anterior aesthetic zone with an implant. Patients were divided into two groups of equal numbers. In the first group, eight implants were immediately placed utilizing the ice cream cone technique, whereas in the second group, eight implants were immediately inserted using vestibular socket therapy into the maxillary …

Effect Of Zinc Oxide Nanoparticle Coating And Artificial Saliva On The Frictional Resistance Of Self-Ligating Ceramic Brackets: An In Vitro Study, Amal Mohammed Al-Hajj Abdo, Reham Ibrahim El-Gazzar, Hagag Mohammed, Ahmed Maher Fouda

Mansoura Journal of Dentistry

Aim: The objective of this research was to investigate how ZnO nanoparticles and artificial saliva influence the frictional resistance in self-ligating ceramic orthodontic brackets while performing sliding mechanics Materials & Methods : A total of sixty maxillary canine brackets were divided into three groups: uncoated self-ligating ceramic brackets, ZnO-coated brackets, and ZnOcoated brackets tested under artificial saliva. The friction produced by sliding was measured using a universal testing machine with a 0.019 × 0.025-inch stainless-steel archwire. One-way ANOVA was used for the statistical analysis, and p-values of ≤ 0.05 were considered significant. Results: The results showed a significant difference in …

Ultrasound-Guided Vs Conventional Intraarticular Injection Of Hyaluronic Acid For Management Of Temporomandibular Joint Internal Derangement: A Randomized Controlled Trial, Muhanad Almufarrih, Muhammad Al-Hawari, Ayman Yaseen, Sally Abdelsameaa

Mansoura Journal of Dentistry

Objective: This study aimed to evaluate the clinical efficacy of using ultrasound guidance during intra-articular injection of hyaluronic acid (HA) in the management of patients with TMJ internal derangement. Material/subjects and Methods: Forty patients complaining of anterior disc displacement with reduction (DDwR) not responsive to conservative treatment were enrolled in this study. The diagnosis was based on the current update of diagnostic criteria for temporomandibular disorders (TMDs). Intra-articular injection of Hyaluronic Acid guided by Ultrasound in the first group and Intra-articular Injection (IAI) of HA conventional way in the second group were performed. Articular pain was evaluated using the visual …

Effect Of Presence Of Cervical Composite Restoration On Intraoral Scanner Precision Of Anterior Laminate Veneer Preparations – An In Vitro Study, Mohamed Tarek Shanshan, Walid Abd El-Ghafar Al-Zordok, Mohamed Hamed Ghazy

Mansoura Journal of Dentistry

Objective

To evaluate the effect of cervical composite restoration on the precision of digital impressions in anterior laminate veneer preparations.

Materials and methods

Forty-eight sound human maxillary central incisors were collected and prepared for anterior laminate veneer restorations following standardized preparation protocol. Specimens were grouped into two equal groups according to the presence of cervical composite restoration: With cervical restoration and without cervical restoration. All preparations were scanned 10 times repeatedly using Primescan intraoral scanner and exported as standard tessellation language (STL) files. Precision was evaluated by calculating root mean square (RMS) values through three-dimensional superimposition analysis using Geomagic Control …

Fracture Resistance Of Monolithic Zirconia Restorations After Endodontic Access Cavity Preparation: Effect Of Repair Method, Isra Osman Mohammed, Esaama. Khedr, Lamia E. Dawood, Walid Abd Elghafar Al-Zordok

Mansoura Journal of Dentistry

objective: The present study was performed to assess the effect of restoration of access cavity with composite resin filling on fracture resistance of multilayered zirconia crowns.

Materials and Methods:

Thirty-three sound human mandibular first molars were prepared and restored with three types of monolithic zirconia crowns: High-Translucent Multilayered Plus (HTML), Yttria Multilayered (YML), and Ultra-Translucent Multilayered (UTML) (N=11 each). Standardized access cavities were prepared through the occlusal surfaces, endodontic treatment was completed, and each access opening was repaired using incremental composite resin. The specimens were subjected to thermocycling (10,000 cycles between 5 °C and 55 °C) and cyclic mechanical loading …

Extended Platelet Rich Fibrin In Lateral Maxillary Sinus Lifting With Simultaneous Implant Placement, Ammar Radwan Samman, Heba Abo-Elfetouh Elsheikh, Walid Abd El-Ghafar Al-Zordk, Noha Ahmed Mansour

Mansoura Journal of Dentistry

Objective: This study aimed to investigate the influence of extended platelet-rich fibrin (e-PRF) when used in lateral maxillary sinus floor augmentation (MSFA), with particular emphasis on implant stability and peri-implant marginal bone loss.

Materials and Methods: Thirteen patients presenting with posterior maxillary tooth loss and a residual bone height of 3–5 mm underwent lateral sinus floor augmentation with concomitant implant placement. A total of 16 implant sites were included and equally allocated into two groups: Group I (n = 8), in which an allograft was applied and covered using a pericardium membrane, and Group II (n = 8), in which …

Effect Of Biodentine And Treated Dentine Matrix On Viability And Proliferation Of Human Dental Pulp Stem Cells, Nada Barakat, Rana El-Qashty, Mohamad E. Helal

Mansoura Journal of Dentistry

Objective: The present study aimed to assess the dose-dependent effect of Biodentine (BD) and human-treated dentin matrix (hTDM) on the proliferation of human dental pulp stem cells (hDPSCs).

Materials and methods: hTDM was prepared and characterized. The concentration of proteins released from BD and hTDM extracts were quantified using the bicinchoninic acid (BCA) assay after 3 and 7 days. hDPSCs were successfully isolated and characterized, then treated with various concentrations of BD extracts (20, 2, and 0.2 mg/mL) and hTDM extracts (100%, 80%, 60%, 40%, 20%, and 10%). Cell viability was determined after 7 days of incubation using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium …

Survival Rate And Prognostic Criteria In Oral Squamous Cell Carcinoma Patients: A Retrospective Analysis, Shihab Ahmed Jassim, Shadi Awny Hasan, Mohamed Mahmoud Anees, Jilan Mohamed Youssef

Mansoura Journal of Dentistry

Objective:

To evaluate overall survival, disease-free survival (DFS), and associated prognostic factors among patients with oral squamous cell carcinoma (OSCC) treated at a tertiary oncology center in Egypt.

Materials/Subjects and Methods: This retrospective cohort study was conducted at the Surgical Oncology Department, Oncology Center, Mansoura University, Egypt. Medical records of patients with histopathologically confirmed primary OSCC diagnosed between January 2011 and December 2021 were reviewed. A total of 140 eligible patients were included. Data collected included demographic characteristics, selected medical history, tumor site, tumor size, TNM stage, histological grade, treatment modality, and follow-up status. Data were entered, managed, and analyzed …

An Attempt For In Situ Bio-Root Regeneration By Using Treated Dentin Matrix And Dental Pulp Stem Cells In Albino Rats, Amani Mohamad, Ahmad A. Emam, Eman Hany, Mohamed Abdulrahman

Mansoura Journal of Dentistry

Objectives: This study was conducted to prepare and characterize root-shaped treated dentin matrix (TDM) scaffolds, and to evaluate their efficacy together with seeded dental pulp stem cells (DPSCs) as potential bio-roots in extraction sockets of rat models.

Materials and Methods: Root-shaped TDM specimens were prepared and characterized. Following extraction of maxillary incisors, the alveolar sockets were implanted with TDM segments measuring 8 mm in length, either alone (TDM group) or seeded with dental pulp stem cells (TDM/DPSCs group). Animals were euthanized at two and six weeks post-implantation, specimens were processed for histological, histochemical and immunohistochemical analyses.

Results: TDM group at …

Chronic Alcohol Consumption Enhances The Differentiation Capacity Of Hematopoietic Stem And Progenitor Cells Into Osteoclast Precursors, Hami Hemati, Madison B. Blanton, Jude Koura, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis. In a rhesus macaque model of voluntary ethanol consumption, CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response. In the present study, those studies were extended to investigate alterations in osteoclast development, which, in postnatal life, differentiate from HSPCs and play a critical role in maintaining bone homeostasis. Spectral flow cytometry revealed a skewing of HSPCs toward granulocyte-monocyte progenitors in the CAC group, consistent with an increased …