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ประสิทธิผลโปรแกรมลดดัชนีมวลกายตามอายุโดยประยุกต์ใช้แบบจำลองความเชื่อด้านสุขภาพในนักเรียนมัธยมศึกษาตอนต้นที่มีภาวะน้ำหนักเกิน, วราภรณ์ คำรศ

Chulalongkorn University Theses and Dissertations (Chula ETD)

ภาวะน้ำหนักเกินเป็นผลจากการมีปฏิสัมพันธ์ที่ซับซ้อนระหว่างกรรมพันธุ์และสิ่งแวดล้อม แบบจำลองความเชื่อด้านสุขภาพเป็นแบบจำลองหนึ่งที่มีการนำไปใช้เพื่อแก้ปัญหาทางสุขภาพได้ผลเป็นอย่างดี แต่ยังไม่พบนำมาประยุกต์ใช้แก้ปัญหาภาวะน้ำหนักเกินของนักเรียนระดับมัธยมศึกษาตอนต้น วัตถุประสงค์การวิจัย เพื่อศึกษาประสิทธิผลโปรแกรมลดดัชนีมวลกายตามอายุโดยประยุกต์ใช้แบบจำลองความเชื่อด้านสุขภาพในนักเรียนมัธยมศึกษาตอนต้นที่มีภาวะน้ำหนักเกิน การศึกษาครั้งนี้เป็นการศึกษาเชิงทดลองแบบสุ่มชนิดกลุ่มมีกลุ่มควบคุม ทำการศึกษากับนักเรียนที่กำลังศึกษาชั้นมัธยมศึกษาตอนต้นปีที่ 1-2 อายุระหว่าง 12-15 ปีที่มีภาวะน้ำหนักเกิน โดยพิจารณาจากดัชนีมวลกายตามอายุ (body mass index-for-age) มีค่า ≥ median +1 SD จาก 48 ห้องเรียน (479 คน) ทำการสุ่มเลือก 24 ห้องเรียน (248 คน) เข้ากลุ่มทดลองได้รับโปรแกรมลดดัชนีมวลกายตามอายุโดยประยุกต์ใช้แบบจำลองความเชื่อด้านสุขภาพเป็นระยะเวลา 6 เดือนและกลุ่มควบคุม 24 ห้องเรียน (231 คน) ได้รับโปรแกรมสุขศึกษาทั่วไปในการเรียนการสอนปกติ จำนวน 6 เดือน แล้วเปรียบเทียบดัชนีมวลกาย ความรอบรู้ด้านสุขภาพและพฤติกรรมสุขภาพเพื่อป้องกันภาวะอ้วน ระหว่างทดลองและกลุ่มควบคุม ผลการศึกษาพบว่าเมื่อวิเคราะห์แบบ Intention-to-Treat หลังเข้าร่วมโครงการวิจัยเดือนที่ 6 กลุ่มทดลองมีระดับดัชนีมวลกายเฉลี่ยลดลงอย่างมีนัยสำคัญทางสถิติเมื่อเปรียบเทียบกับกลุ่มควบคุม -2.88 กก./ม.2 (95% CI = -3.01 ถึง -2.75, p-value < 0.001) คะแนนความรอบรู้ด้านสุขภาพเฉลี่ยเพิ่มขึ้นอย่างมีนัยสำคัญทางสถิติเมื่อเปรียบเทียบกับกลุ่มควบคุม 27.79 คะแนน (95% CI = 26.98 ถึง 28.60, p-value < 0.001) และคะแนนพฤติกรรมสุขภาพโดยรวมเฉลี่ยเพิ่มขึ้นอย่างมีนัยสำคัญทางสถิติเมื่อเปรียบเทียบกับกลุ่มควบคุม 23.61 คะแนน (95% CI = 22.68 ถึง 24.54, p-value < 0.001) แบบจำลองความเชื่อด้านสุขภาพนำมาประยุกต์พัฒนาโปรแกรมเพื่อลดดัชนีมวลกายในนักเรียนระดับมัธยมศึกษาตอนต้นได้ผลเป็นอย่างดี แม้ว่าจะจัดโปรแกรมต่อเนื่องในระยะสั้น 6 เดือน

The Skin Microbiome: Impact Of Modern Environments On Skin Ecology, Barrier Integrity, And Systemic Immune Programming, Susan Prescott, Danica-Lea Larcombe, Alan Logan, Christina West, Wesley Burks, Luis Caraballo, Michael Levin, Eddie Van Etten, Pierre Horwitz, Anita Kozyrskyj, Dianne Campbell

Research outputs 2014 to 2021

Skin barrier structure and function is essential to human health. Hitherto unrecognized functions of epidermal keratinocytes show that the skin plays an important role in adapting whole-body physiology to changing environments, including the capacity to produce a wide variety of hormones, neurotransmitters and cytokine that can potentially influence whole-body states, and quite possibly, even emotions. Skin microbiota play an integral role in the maturation and homeostatic regulation of keratinocytes and host immune networks with systemic implications. As our primary interface with the external environment, the biodiversity of skin habitats is heavily influenced by the biodiversity of the ecosystems in which …

Comparison Of Microscopy, Elisa, And Real-Time Pcr For Detectionof Giardia Intestinalis In Human Stool Specimens, Yunus Emre Beyhan, Zeynep Taş Cengi̇z

Turkish Journal of Medical Sciences

Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-ether sedimentation technique and microscopic examinations were carried out on wet mount slides. A commercially available ELISA kit (Giardia CELISA, Cellabs, Brookvale, Australia) was used for immunoassay. DNA was extracted from fecal samples of about 200 mg using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Hilden, …

Insights Into The Ecology, Genetics And Distribution Of Lucanus Elaphus Fabricius (Coleoptera: Lucanidae), North America's Giant Stag Beetle, Michael D. Ulyshen, Louis G. Zachos, John O. Stireman Iii, Thomas N. Sheehan, Ryan C. Garrick

Biological Sciences Faculty Publications

1. Little is known about the biology or conservation status of Lucanus elaphus Fabricius in North America despite well-documented declines of a related species, Lucanus cervus (L.), in Europe. This study provides information critical to developing conservation plans for L. elaphus including the species’ larval substrate requirements, genetic data and range-wide estimates of habitat suitability. 2. In Mississippi floodplain forests, larval L. elaphus were recovered from a wide range of log sizes and rot types and were either found tunnelling within the wood or feeding beneath logs at the soil–wood interface. The species appears to require 1–2 years to complete …

Prediction Of Atopy Via Total Immunoglobulin E Levels Andskin Prick Tests In Patients With Psoriasis, Emi̇ne Sümeyye Ünal, Ülker Gül, Adi̇le Berna Dursun, Ferda Öner Erkekol

Turkish Journal of Medical Sciences

Background/aim: Although the etiopathogeneses of psoriasis and atopy appear to be different, psoriasis has been found to be associated with atopy and atopic dermatitis. In this study, we aimed to determine the role of atopy by examining the medical history and clinical and laboratory findings of patients with psoriasis. Materials and methods: Patients with psoriasis, asthma patients, and healthy volunteers were included in the study. Serum total immunoglobulin E (IgE) levels were obtained, and prick tests were administered to all groups. Results: Psoriatic patients demonstrated percentages of atopy history (21.3%) that were higher than those of the healthy subjects (15.7%). …

Modifications Of Mice Gut Microflora Following Oral Consumption Oflactobacillus Acidophilus And Bifidobacterium Bifidum Probiotics, Hossein Khavari-Daneshvar, Maryam Mosavi, Hamid Khodayari, Ebrahim Rahimi, Peyman Ranji, Amir Hossein Mohseni, Reyhaneh Mahmudian, Farzad Shidfar, Shahram Agah, Ali Mohammad Alizadeh

Turkish Journal of Medical Sciences

Background/aim: Thirty male BALB/c mice were equally divided into three groups: control, L. acidophilus, and B. bifidum for the assessment of the probiotics' stability in the gut microflora. Materials and methods: First, the gut microflora of the mice was checked every 3 days (days 3, 6, 9, and 12) without probiotic consumption, and then the mice were daily given orally 1.5 g of probiotics in 30 cc of drinking water. The consumption of probiotics was then stopped for recovery and then the consumption continued for 5 months. Results: On day 9 after the consumption of the probiotics, L. acidophilus and …

Evaluation Of Cytotoxic, Membrane, And Dna Damaging Effects Of Thymus Revolutuscélak Essential Oil On Different Cancer Cells, Aysun Özkan, Ayşe Erdoğan

Turkish Journal of Medical Sciences

Background/aim: In this study, we evaluated Thymus revolutus Célak essential oil and its two main constituents, γ-terpinene and p-cymene, as potential oxidative agents against lung cancer and epidermoid carcinoma cells. Materials and methods: Cell viability assessment was made by CellTiter-Blue® cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay after treatment with 5?600 ?g/mL concentrations of essential oil, γ-terpinene, and p-cymene. Malondialdehyde and 8-hydroxy-2'-deoxyguanosine levels in parental H1299, epirubicin-resistant (drug-resistant) H1299, A549, and A431 cells were also assayed after essential oil, γ-terpinene, and p-cymene had been administered for 24 h. Glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GRx), and glucose …

The Effect Of Trimetazidine On Renal Oxidative Stress In Partialand Complete Ureteral Obstructions In A Rat Model, Sema Nur Ayyildiz, Ali̇ Ayyildiz, Erdal Benli̇, Selma Cirrik, Rüştü Cankon Germi̇yanoğlu, Tevfi̇k Noyan, Abdullah Çirakoğlu

Turkish Journal of Medical Sciences

Background/aim: Renal oxidative stress occurs in ureteral obstructions. The purpose of this study was to investigate the effect of the antioxidant and antiischemic agent trimetazidine (TMZ) on oxidative stress following ureteral obstruction. Materials and methods: Ten groups were established. Sham groups were checked as controls after 1 and 3 weeks. The other 8 groups had partial or complete ureteral obstruction while receiving or not receiving trimetazidine (TMZ) at 5 mg/kg daily and were evaluated after either 1 week or 3 weeks. Creatinine and cystatin C measurements were performed in the serum. Malondialdehyde, myeloperoxidase, catalase, and glutathione peroxidase activity were measured …

The Reno-Protective Effects Of Atorvastatin In Crush Syndrome And Rhabdomyolysis:Is There A Dilemma?, Demet Acar, Mustafa Gülpembe, Can Gökay Yildiz, Emi̇ne Nur Özdamar, Kerem Açikgöz, Ahmet Çağlar, Başar Cander

Turkish Journal of Medical Sciences

Background/aim: We aim to determine the effects of low-dose atorvastatin treatment together with crush fluid resuscitation on renal functions and muscle enzyme levels in a rat model of crush syndrome. Materials and methods: The study involved female Wistar Albino rats weighing 250-300 g that were housed with free access to food and water. The crush model was obtained by compression. Rats were randomly divided into four groups: control (C) group, atorvastatin + crush fluid (ACF) group, crush fluid (CF) group, and hypertonic saline (%3) + mannitol + sodium bicarbonate (SM) group. Blood was obtained at 24, 48, and 72 h, …

Islet Transplantation Promotes Podocyte Regeneration In A Model Of Diabetic Nephropathy, Ziqiang Xu, Jie Fan

Turkish Journal of Medical Sciences

Background/aim: The purpose of this study was to observe whether islet transplantation could induce glomerular parietal epithelial cells to express podocyte proteins in a rat model of streptozotocin-induced diabetic nephropathy (DN).Materials and methods: A total of 18 rats were given single injections of streptozotocin to induce a DN model. Eight weeks after the modeling, successfully established DN rats were divided into three groups: an untreated group (DN group), an islet-transplanted group (IT group), and an insulin group (IN group). The islets cells were isolated from donor rats and surgically transplanted from under the kidney capsule in the IT group. Four …

The Effects Of Exercise Preconditioning On Focal Ischemic Stroke, Gillian Grohs

Theses and Dissertations--Medical Sciences

Cleaved fragments of the extracellular matrix protein perlecan have been shown to promote neuroprotection and repair after ischemic stroke. The cysteine proteases cathepsin B and L as well as the metalloprotease bone morphogenic protein 1 (BMP-1) are capable of releasing the biologically active C-terminal laminin-like globular domain (LG3) of perlecan. Exercise, a known method of reducing stroke risk and severity, has been shown to increase the expression of some proteases associated with perlecan processing. Using a transient distal middle cerebral artery occlusion (MCAo) model for focal ischemic stroke we show that while 7 days of running only slightly decreased infarct …

Factors Regulating Features Of Metabolic Syndrome, Sonja S. Pijut

Theses and Dissertations--Pharmacy

The collective presence of central obesity, low HDL-cholesterol, and elevated triglycerides, blood pressure, and fasting blood glucose constitutes Metabolic Syndrome (MetS), a disease state that increases the risk of cardiovascular disease (CVD) and Type 2 Diabetes Mellitus (T2DM). Nonalcoholic fatty liver disease (NAFLD), present in up to 90% of obese adults, is also linked to MetS. As in CVD, disruptions in cholesterol metabolism play a contributing role in the development of T2DM and NAFLD. Genes involved in cholesterol synthesis, secretion, and catabolism are diurnally regulated in the liver and adipose. Disruptions in the sleep-wake cycle are thought to potentiate metabolic …

Telomerase Reverse Transcriptase In Atherosclerosis, Hua Qing

Theses and Dissertations--Pharmacology and Nutritional Sciences

Telomerase reverse transcriptase (TERT) is the catalytic subunit of telomerase and the limiting factor for the enzyme activity. The expression of TERT and telomerase activity is increased in atherosclerotic plaques. However, the role of TERT dysregulation during atherosclerosis formation remains unknown.

The work herein first identified a multi-tiered regulation of TERT expression in smooth muscle cells (SMC) through histone deacetylase (HDAC) inhibition. HDAC inhibition induces TERT transcription and promoter activation. At the protein level in contrast, HDAC inhibition decreases TERT protein abundance through enhanced degradation, which decreases telomerase activity and induces senescence. Furthermore, during vascular remodeling in vivo, TERT protein …

Insulin Actions On Hippocampal Neurons, Shaniya Maimaiti

Theses and Dissertations--Pharmacology and Nutritional Sciences

Aging is the main risk factor for cognitive decline. The hippocampus, a brain region critical for learning and memory formation, is especially vulnerable to normal and pathological age-related cognitive decline. Dysregulation of both insulin and intracellular Ca2+ signaling appear to coexist and their compromised actions may synergistically contribute to neuronal dysfunction with aging. This dissertation focused on the interaction between insulin, Ca2+ dysregulation, and cognition in hippocampal neurons by examining the contributions of insulin to Ca2+ signaling events that influence memory formation. I tested the hypothesis that insulin would increase cognition in aged animals by altering Ca2+-dependent physiological mechanisms involved …

Neuroinflammation In Alzheimer's Disease And Vascular Cognitive Impairment, Erica M. Weekman

Theses and Dissertations--Physiology

It was once believed that the brain was immunologically privileged with no resident or infiltrating immune cells; however, now it is understood that the cells of the brain are capable of a wide range of inflammatory processes and phenotypes. Inflammation in the brain has been implicated in several disease processes such as Alzheimer’s disease (AD) and vascular cognitive impairment and dementia (VCID); however, the role of inflammation in these two dementias is poorly understood.

When we stimulated a pro-inflammatory phenotype with an adeno-associated viral vector in a transgenic mouse model of AD that develops Aβ plaques, we saw a pro-inflammatory …

Steroidogenesis In The Green Anole Lizard Brain And Gonad, Christine Peek

All Graduate Theses, Dissertations, and Other Capstone Projects

Seasonally breeding animals reproduce during certain times of the year and, subsequently, behaviors, steroid hormone levels, and brain morphology change. The green anole lizard (Anolis carolinensis) is an excellent model to study the regulation of steroid hormone production because they have distinct hormonal and behavioral differences between sexes and seasons. As in other vertebrates, steroidogenesis in anoles is under the control of the hypothalamus-pituitary-gonadal (HPG) axis. We tested the hypothesis that natural variations in steroid hormone levels between sexes and seasons are mediated within the brain and gonad by examining four genes involved in steroidogenesis: StAR, Cyp17α1, HSD17β3, and Cyp19α1. …

The Mitochondrial Ca(2+) Uniporter: Structure, Function, And Pharmacology., Jyotsna Mishra, Bong Sook Jhun, Stephen Hurst, Jin O-Uchi, György Csordás, Shey-Shing Sheu

Center for Translational Medicine Faculty Papers

Mitochondrial Ca(2+) uptake is crucial for an array of cellular functions while an imbalance can elicit cell death. In this chapter, we briefly reviewed the various modes of mitochondrial Ca(2+) uptake and our current understanding of mitochondrial Ca(2+) homeostasis in regards to cell physiology and pathophysiology. Further, this chapter focuses on the molecular identities, intracellular regulators as well as the pharmacology of mitochondrial Ca(2+) uniporter complex.

From Dose To Response: In Vivo Nanoparticle Processing And Potential Toxicity, Uschi M. Graham, Gary Jacobs, Robert A. Yokel, Burtron H. Davis, Alan K. Dozier, M. Eileen Birch, Michael T. Tseng, Günter Oberdörster, Alison Elder, Lisa Delouise

Pharmaceutical Sciences Faculty Publications

Adverse human health impacts due to occupational and environmental exposures to manufactured nanoparticles are of concern and pose a potential threat to the continued industrial use and integration of nanomaterials into commercial products. This chapter addresses the inter-relationship between dose and response and will elucidate on how the dynamic chemical and physical transformation and breakdown of the nanoparticles at the cellular and subcellular levels can lead to the in vivo formation of new reaction products. The dose-response relationship is complicated by the continuous physicochemical transformations in the nanoparticles induced by the dynamics of the biological system, where dose, bio-processing, and …

Cardiac Chemical Exchange Saturation Transfer Mr Imaging Tracking Of Cell Survival Or Rejection In Mouse Models Of Cell Therapy, Ashley L. Pumphrey, Shaojing Ye, Zhengshi Yang, Jennifer Simkin, John C. Gensel, Ahmed Abdel-Latif, Moriel H. Vandsburger

Saha Cardiovascular Research Center Faculty Publications

Purpose:

To examine whether cardiac chemical exchange saturation transfer (CEST) imaging can be serially and noninvasively used to probe cell survival or rejection after intramyocardial implantation in mice.

Materials and Methods:

Experiments were compliant with the National Institutes of Health Guidelines on the Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee. One million C2C12 cells labeled with either europium (Eu) 10-(2-hydroxypropyl)-1,4,7-tetraazacyclododecane-1,4,7-triacetic acid (HP-DO3A) or saline via the hypotonic swelling technique were implanted into the anterior-lateral left ventricular wall in C57BL/6J (allogeneic model, n = 17) and C3H (syngeneic model, n = 13) mice. Imaging …

A Rational Design Of A Selective Inhibitor For Kv1.1 Channels Prevalent In Demyelinated Nerves That Improves Their Impaired Axonal Conduction, Ahmed Al-Sabi, Declan Daly, Patrick Hoefer, Gemma K. Kinsella, Charles Metais, Mark Pickering, Caroline Herron, Seshu Kumar Kaza, Kieran Nolan, J. Oliver Dolly

Articles

K+ channels containing Kv1.1 α subunits, which become prevalent at internodes in demyelinated axons, may underlie their dysfunctional conduction akin to muscle weakness in multiple sclerosis. Small inhibitors were sought with selectivity for the culpable hyper-polarizing K+ currents. Modeling of interactions with the extracellular pore in a Kv1.1-deduced structure identified diaryldi(2-pyrrolyl)methane as a suitable scaffold with optimized alkyl ammonium side chains. The resultant synthesized candidate [2,2′-((5,5′(di-p-topyldiaryldi(2-pyrrolyl)methane)bis(2,2′carbonyl)bis(azanediyl)) diethaneamine·2HCl] (8) selectively blocked Kv1.1 channels (IC50 ≈ 15 μM) recombinantly expressed in mammalian cells, induced a positive shift in the voltage dependency of K+ current activation, and slowed its kinetics. It …

The Effects Of Perinatal Oxycodone Exposure On The Stress Axis And Neurobehavior, Thitinart Sithisarn

Theses and Dissertations--Physiology

Opiate addiction is now a major public health problem. Pregnant women continue to use opiates during gestation; up to 5.4% of pregnant women report using illicit drugs during pregnancy. Previous studies have shown that perinatal insults and exposure to opiates such as morphine in utero can affect the development of the hypothalamic-pituitary-adrenal (HPA)-axis of the offspring and are associated with higher risk of developing neurobehavioral problems. Oxycodone, a semisynthetic putative kappa opioid receptor and partial mu opioid receptor agonist is now one of the most frequently abused pain killers during pregnancy, however limited data are available regarding whether and how …

อัตราอุบัติการณ์และปัจจัยที่เกี่ยวข้องกับการบาดเจ็บจากการทำงานของสัตวแพทย์ในสถานพยาบาลสัตว์ ในเขตกรุงเทพมหานคร, นภัฐมณ มโนรัตน์

Chulalongkorn University Theses and Dissertations (Chula ETD)

การศึกษานี้เป็นการศึกษาวิจัยเชิงพรรณนาแบบตัดขวาง มีวัตถุประสงค์เพื่อศึกษาอัตราอุบัติการณ์และลักษณะของการบาดเจ็บจากการทำงานของสัตวแพทย์ภายใน 1 ปีย้อนหลัง และปัจจัยที่มีความเกี่ยวข้องกับการบาดเจ็บจากการทำงานของสัตวแพทย์ที่ทำงานในสถานพยาบาลสัตว์ ในกรุงเทพมหานคร โดยแจกแบบสอบถามให้แก่สัตวแพทย์ที่ปฏิบัติงานประจำในสถานพยาบาลสัตว์ ในเขตกรุงเทพมหานครที่ทำงานมาแล้วอย่างน้อย 1 ปี จำนวน 395 คน วิเคราะห์โดยใช้สถิติเชิงพรรณนา ได้แก่ ความถี่ ร้อยละ ค่าเฉลี่ย ส่วนเบี่ยงเบนมาตรฐาน และสถิติเชิงอนุมาน ได้แก่ Chi-square test, Fisher's exact test และ Independent t-test การศึกษานี้พบว่า อัตราอุบัติการณ์การบาดเจ็บที่สำคัญของสัตวแพทย์เท่ากับ 280 ครั้งต่อล้านชั่วโมงการทำงาน หรือ ใน 1 ปี จะมีการบาดเจ็บจนมีข้อจำกัดในการทำงาน หรือหยุดงานหรือต้องไปพบแพทย์ 56.22 ครั้ง เมื่อเทียบกับสัตวแพทย์ที่ทำงานเต็มเวลา 100 คน การบาดเจ็บเกิดจาก สุนัข และแมวกัดมากที่สุด อวัยวะที่ได้รับบาดเจ็บมากที่สุด คือ มือ ลักษณะของบาดแผลที่พบมากสุดคือ แผลถลอก และแผลฉีกขาด การบาดเจ็บมักเกิดขึ้นในช่วงการตรวจวินิจฉัย เมื่อบาดเจ็บแล้วสัตวแพทย์ไปพบแพทย์ร้อยละ 69.67 และเกิดข้อจำกัดในการทำงานร้อยละ 56.30 ส่วนผู้ที่บาดเจ็บและจำเป็นต้องหยุดงานมีระยะเวลาหยุดงานเฉลี่ย 3.45 วัน ปัจจัยที่พบความสัมพันธ์กับการบาดเจ็บของสัตวแพทย์ ได้แก่ ปีที่จบการศึกษา (p-value = 0.003) อายุ (ปี) (p-value < 0.001) ดัชนีมวลกาย (p-value < 0.001) การเป็นโรคภูมิแพ้ (p-value = 0.001) การนอนน้อยกว่า 7 ชั่วโมง (p-value = 0.022) การรับประทานยาที่มีฤทธิ์ทำให้ง่วง (p-value = 0.003) การออกกำลังกาย (p-value = 0.024) ประเภทของสัตว์ที่สัมผัสในงาน (p-value < 0.001) ประสบการณ์การทำงานตั้งแต่ 6 ปีขึ้นไป (p-value < 0.001) ระยะเวลาการทำงานมากกว่า 40 ชั่วโมงต่อสัปดาห์ (p-value = 0.035) การอบรมเกี่ยวกับความปลอดภัยในการทำงาน (p-value = 0.038) พฤติกรรมในการทำงาน ได้แก่ มีการจับและควบคุมสัตว์อย่างรัดกุมขณะตรวจหรือทำหัตถการ (p-value = 0.036) ทิ้งเข็มใช้แล้วลงถังขยะสำหรับทิ้งของมีคมสม่ำเสมอ (p-value = 0.01) สภาพแวดล้อม อันได้แก่ ความเพียงพอของผู้ช่วยจับสัตว์ (p-value = 0.01) อุปกรณ์ควบคุมสัตว์ที่เพียงพอ (p-value < 0.001) แสงสว่างในห้องทำงานที่เพียงพอ (p-value = 0.018) ผู้วิจัยแนะนำให้มีการปรับระยะเวลาการทำงานและระยะเวลานอนให้เหมาะสม ร่วมกับสร้างความตระหนัก ให้ความรู้ เพิ่มการถ่ายทอดประสบการณ์จากผู้ที่มีประสบการณ์ รวมถึงการเตรียมสถานที่ตรวจให้เหมาะสม น่าจะนำไปสู่การลดอุบัติการณ์การบาดเจ็บในสัตวแพทย์

Effects Of Genistein On Oxidative Stress And Inflammation In Acute Pancreatitis Mice, Jumlongluk Sriko

Chulalongkorn University Theses and Dissertations (Chula ETD)

Genistein (GEN), the most abundant isoflavones in soy, has similar structure to human estradiol. It has been reported the potent properties of GEN on antioxidant and anti-inflammation. The aim of this study was to investigate the effects of GEN on oxidative stress, inflammatory, and histopathology in acute pancreatitis induced by L-arginine in mice. Male ICR mice were randomly divided into 4 groups (6 mice in each group). Control (Con) group: mice were injected with 2% dimethyl sulfoxide (DMSO) once daily for 4 days, via IP. Acute pancreatitis (AP) group: mice were injected 2 times (1 hour interval) of 350 mg/100 …

Surface Proteomics Of Leptospira Interrogans Serovar Pomona, Praparat Thaibankluay

Chulalongkorn University Theses and Dissertations (Chula ETD)

Leptospirosis is a re-emerging zoonosis of global distribution including Thailand. It caused by pathogenic Leptospira. The pathogenesis is not clear. Surface-exposed proteins (PSEs) are first part for interactions with host cells, immune system and should be potential targets for vaccine development. To date, many researches study only individual PSEs but the studies of surface proteomic is less. The study of surface proteomic will be important information for pathogenesis studies and search for vaccine candidate. This study aimed to identify surface proteomics of pathogenic Leptospira by surface biotinylation and surface shaving using proteinase K that has been used to identify surface …

The Potential Role Of Oxidative Stress In Reduced Uterine Perfusion Pressure Vascular Reactivity, Abigail J. Gindlesberger

Williams Honors College, Honors Research Projects

Preeclampsia is a hypertensive disease of pregnant women that is known to cause detrimental physiological complications to both the mother and fetus. The hypertension hypothesized to result from endothelial dysfunction may be improved therapeutically by increasing Vascular Endothelial Growth Factor ligand concentration and it’s Vascular Endothelial Growth Factor Receptor 2 (VEGF and VEGFR2). Together, this receptor pathway may help overall vasodilation of key blood vessels linking the mother and fetal placental unit, allowing for increased clinical pathologies of both. Previous studies have also linked increased reactive oxygen species (ROS) production in preeclamptic pregnancies to the damaged endothelial tissues of the …

Reliability Of Micro Mechanical Low Frequency Energy Harvesters For Implanted Cardiac Pacemakers, Sunny Jay

Theses

The aim of this project was to develop methods to assess the suitability and reliability of silicon-based piezoelectric micro-electro-mechanicai systems (MEMS) energy harvesters to capture the kinetic energy of the heartbeat in an implanted cardiac pacemaker. The major challenges of the work were that harvesters for vibration frequencies below 100 Hz have not been widely reported and the system requires reliable operation over a long lifetime. In this work device modelling was used for harvester design assessment. Experimental test methods and equipment were developed to produce data to validate the device models and to assess device reliability. A 2D analytical …

In Vitro Three-Dimensional Skin Tissue Constructs, Tinashe Dereck Gombedza

Theses

Human skin provides fortification against peripheral threats which may compromise the integrity and health of the human body. Its presence as the largest human organ forms a protective barrier making it an effective first line of defence against pathogenic, chemical and physical damage. Tissue engineering technology has enabled the development of in vitro three-dimensional (3D) organotypic skin cultures in order to understand the skin's physiology and architecture. This advancement has enabled the use of 3D skin model platforms to study skin diseases and conditions as well as facilitate drug discovery, clinical research and cosmetic product development.

In this study, an …

Effects Of Ankaferd Bloodstopper On Dermal Healing In Diabetic Rats, Sertaç Aktop, Burcu Çevreli̇, Deni̇z Genç, Emel Serdaroğlu Kaşikçi, Ni̇l Çomunoğlu Üstündağ, Noushi̇n Zi̇bandeh, Eli̇f Merve Özcan, Gökhan Göçmen, Mehmet Kami̇l Göker, İsmai̇l Tayfun Uzbay, Tunç Akkoç

Turkish Journal of Medical Sciences

Background/aim: Diabetes mellitus inhibits wound-induced angiogenesis, impairs the wound healing process, and leads to the development of chronic wounds. Ankaferd BloodStopper (ABS) is a new and promising local haemostatic agent. Although the mechanism of ABS-mediated haemostasis is well established, little is known about the associated histological and biochemical tissue reactions. The aim of this study was to evaluate the effects of this new-generation local haemostatic agent on short-term soft-tissue healing in streptozotocin (STZ)-treated rats. Materials and methods: The 24 Wistar albino rats used in this study were divided into STZ-treated (STZ, n = 12) and nontreated groups (control, n = …

Depression And Parkinson Disease: Prevalence, Temporal Relationship, And Determinants, Hale Yapici Eser, Hati̇ce Ayşe Bora, Asli Kuruoğlu

Turkish Journal of Medical Sciences

Background/aim: Comorbidity of depression in Parkinson disease (PD) is a major factor that changes patients? quality of life. However, the neurobiological and sociodemographic risk factors for this comorbidity are not well studied. In this study, we aimed to define the prevalence, temporal relationship, and psychosocial and clinical determinants of depression comorbid with PD. Materials and methods: Fifty-five PD patients were evaluated with SCID, a data form that assessed sociodemographic and PD-related variables, UPDRS III, HAM-D, HAM-A, MMSE, and the Apathy Evaluation Scale. Results: Depression (lifetime: 45.5%, last month: 25.5%, before PD: 20%) was the most frequent psychiatric diagnosis. The major …

Antioxidants Status In Type 2 Diabetic Patients In Morocco, Rachid Eljaoudi, Naoual Elomri, Meryem Laamarti, Yahia Cherrah, Taoufik Amezyane, Driss Ghafir, Azeddine Ibrahimi

Turkish Journal of Medical Sciences

Background/aim: Type 2 diabetes is a heterogeneous and multifactorial metabolic disorder with some relationship to oxidative stress (OS). Since no studies were conducted in the Moroccan population, this clinical investigation aimed at evaluating the antioxidants status in Moroccan patients with type 2 diabetes. Materials and methods: Blood samples of 60 type 2 diabetic patients and 40 healthy controls subjects were analyzed for determination of glycemia, hemoglobin, CRP, glycated hemoglobin, lipid parameters, malondialdehyde (MDA), vitamins E and C, copper (Cu), zinc (Zn), and selenium (Se). Results: CRP and triglycerides were higher in the diabetic group while high-density lipoprotein levels were significantly …