Digital Commons Network^™

Site Fidelity, Seasonality, Movement, And Fish Associates Of Sand Tigers Carcharias Taurus In North Carolina, Ara L. Mcclanahan

Electronic Theses and Dissertations

Sand tigers Carcharias taurus are mackerel sharks with a disjunct global distribution in tropical and temperate seas. In the northwest Atlantic, sand tigers migrate along the coast of the eastern United States from the Gulf of Maine to Florida and occupy structurally complex benthic habitats. The long-term, underwater camera installation, SharkCam, at Frying Pan Tower, off Cape Fear, North Carolina, USA, sits atop an expanse of hard bottom reef frequented by sand tigers. The citizen-science platform Spot A Shark USA (SAS USA) uses images of sand tigers to identify and track individuals. This study utilized image encounters from SAS USA …

A Multi-Isotope Investigation Of Mammoth Diet, Habitat, And Movement Patterns In The Central Great Plains, U.S.A., Jacob Mccloskey

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

During the Last Glacial Maximum (LGM), the Great Plains in Nebraska consisted of wooded parklands and grasslands, whereas spruce (Picea) forests, parklands, and steppe grasslands dominated east of the Missouri River. I analyzed bulk carbon (δ¹³C), oxygen (δ¹⁸O), and strontium (⁸⁷Sr/⁸⁶Sr) isotopes from tooth enamel in isolated mammoth (Mammuthus) molars to compare diet and movement patterns in two late Pleistocene assemblages: one in Nebraska (n = 24), and one in western Iowa and Missouri (n = 17). Furthermore, I identified a morphometric subset of molars as …

Evaluation Of Performance Characteristics And Groundwater Contamination Risks Associated With On-Farm Swine Carcass Disposal Via Composting And Shallow Burial With Carbon, Gustavo Castro Garcia

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation evaluates the performance of three on-farm swine carcass disposal methods: whole carcass composting (WCC), ground carcass composting (GCC), and shallow burial with carbon (SBC), focusing on biosecurity factors and groundwater contamination risks. Foreign animal diseases (FADs), such as African swine fever and classical swine fever, pose severe economic and animal well-being risks if introduced to the United States. With confirmation of an FAD, swine movement will be halted, creating an urgent need for practical, biosecure, and environmentally responsible on-farm disposal strategies for swine carcasses. Nebraska, as a leading swine-producing state, exemplifies the vulnerability of high-density livestock regions to …

Educating The New Generation Of Food Scientists Through Innovative Learning Experiences, Karen Andrea Nieto Flores

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Food science provides an opportunity to engage high school students in Science, Technology, Engineering, and Mathematics (STEM) by connecting classroom concepts to real-world applications. Despite the field’s relevance to public health, food safety, sustainability, and innovation, food science remains largely absent from high school curricula, and few students recognize it as a viable STEM pathway. This research addressed this gap by evaluating immersive summer camp experiences and teacher-focused curriculum review as strategies to increase food science awareness and interest among students and educators. A literature review traced the historical development and current landscape of K–12 food science education, identifying barriers …

Egg White Hydrolysate In Hypertension: A Mechanistic Investigation Of Gut Microbiota Dependent And Independent Pathways, Emerson David Nolasco

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Cardiovascular diseases are one of the leading causes of mortality worldwide in which hypertension is a risk factor. Present pharmacological interventions require long-term adherence to the therapy and are often associated with undesirable side effects. Moreover, treatments have overlooked the role of the microbiome in the development of hypertension. Egg white hydrolysate (EWH) has exhibited antihypertensive properties with potential to be used as a natural alternative for the prevention and management of hypertension. However, EWH peptide’s effect on the gut microbiome remains yet to be elucidated. Studies have described that a portion of the ingested protein can remain undigested as …

Functional Genomics Of Phosphatidylcholine Biosynthesis In Saccharomyces Cerevisiae, Amanda Marie Maliva

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Phosphatidylcholine (PtdCho) is a prominent structural building block of cell and organelle membranes in animal, plant, and fungal cells and some bacteria. Due to its abundant nature in the plasma membrane and intracellular organelle membranes in eukaryotes, the biosynthesis of PtdCho must remain regulated to preserve the correct membrane lipid composition. Dysregulations in PtdCho metabolism can lead to mitochondrial dysfunction, cell death, and the development of diseases including cancer, atherosclerosis, and hepatic diseases. Knowledge of PtdCho has remained limited to the genes involved in its three biosynthetic pathways (Kennedy (K), methylation (M), and acyltransferase (A)), despite the growing realization of …

Aptamer Selection And Development Of Electrochemical Aptamer-Based Sensors For Neuropeptide Y, Evelyn Adela Carreto Guevara

Department of Chemistry: Dissertations, Theses, and Student Research

In this thesis, we report the results of four aptamers obtained from an aptamer selection process called the systematic evolution of ligands by exponential enrichment (SELEX) with affinity to Neuropeptide Y (NPY). NPY is a highly abundant peptide transmitter with widespread distribution throughout the body which affects various physiological functions. These functions include energy homeostasis, appetite regulation, stress response, and anxiety regulation. Dynamic monitoring of NPY levels is crucial for better understanding behaviors in individuals with neurological disorders associated with NPY. Aptamers are synthetic oligonucleotides obtained through SELEX which have a binding affinity to a target of interest when in …

Breaking Confirmatory Spells By Implementing Research- Integrated Cures, J. A.C. Vriezen

Biological Sciences: Faculty Publications

Differences in economic and social growth and academic equity, experi ences, and opportunities have left an achievement gap in STEM for underprivileged students. Course-based Undergraduate Research Experiences (CUREs) have attempted to answer these inequalities by leveling the playing field or by offering course-based research opportunities with minimal requirements for background knowledge. When we ask novel questions, more students get the experience of performing research, which lowers thresholds to pursue research and increases participation. Although the learning outcomes have been largely positive, there is a concern that many CUREs are still too scaffolded. In contrast, I propose to develop exploratory, hypothesis-driven …

Local Adaptation In The Pinus Palustris Ground Layer, Jason Paul Joines

All Dissertations

Population survival or extirpation may be dependent on adaptation to local environmental conditions. I hypothesized that populations of species with large ranges would be locally adapted because of the variety of environments that different populations experience, that spatial patterns of local adaptation would correspond to patterns of endemism because processes driving local adaptation may also lead to speciation, and that the extent of local adaptation would be stronger in species with little gene flow among populations than in those with extensive gene flow because of the relative strength of selection necessary to overcome gene flow. I tested these hypotheses using …

B.R. Wells Arkansas Rice Research Studies 2024, J. Hardke, X. Sha, N. Bateman

Arkansas Agricultural Experiment Station Research Series

Research reports contained in the publication may represent preliminary or only a single year of results; therefore, these results should not be read as a basis for long-term recommendations.

Several research reports in this publication will appear in other University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas rice producers of all the research being conducted with funds from the rice check-off program. This publication also contains research funded by industry, federal, and state agencies.

Use of products …

Elucidating The Mechanisms Underlying A Functional Plant-Arbuscular Mycorrhizal Symbiosis Using Omics Approaches, Jasmine Gill

All Dissertations

Arbuscular mycorrhizal fungi (AMF) are obligate biotrophs forming symbiotic association with plants. The plant-AMF symbiosis has gained much attention due to its ability to improve plant nutrient uptake, especially phosphorus, which is often inadequately available to plants due to its limited mobility and complex formation in many soils. Phosphorus (P) is a crucial nutrient for plants, and most phosphate fertilizers are derived from phosphate rock, a finite natural resource. Thus, optimizing P uptake is vital for sustaining crop yields and addressing the needs of a growing global population. Plant-AMF symbioses are significant in this context, as AMF can acquire P …

Breeding Biofortified Protein Rich Organic Pulses For Better Human Health, Sonia Salaria

All Dissertations

Pulse crops are dry grains of legumes, the second most consumed plant crops in the world after cereals. These crops are one of the primary sources of plant-based protein and contain high concentrations of pre-biotic carbohydrates, minerals and vitamins. Recognizing the health benefits of pulses intake, their demands as sustainable food and changes in people’s diet preferences for plant-based protein have increased awareness for pulses consumption. With this scenario, improving the nutritional profile of pulse crops for rich nutrition has been an opportunity for breeders to explore and tailor the available germplasm to develop nutrient dense pulses. Furthermore, the genomic …

Alternative Therapeutics For Human Breast Cancer: Nutraceutical Underpinnings, In Vitro Metabolomic Profiling, Treatment Efficacy, And Contextual Ethical Implications, Aubrey A. Mattingly

All Dissertations

Breast cancer remains one of the most prevalent diseases worldwide, yet therapeutic equity is still lacking. Standard treatments including surgery, radiotherapy, chemotherapy, and long-term hormone therapy. These approaches are often accompanied by significant side effects. While newer methods such as immunotherapy and targeted therapies have emerged, chemotherapy protocols and incorporation have remained largely unchanged in the past decades. In this context, nutraceuticals represent an alternative or adjuvant to therapy worthy of exploration. The reviewed literature highlights the anti-carcinogenic activity in selected nutraceuticals, marjoram, thyme and persimmon, considering their phytochemical constituents and secondary metabolites. Metabolomic profiling using the Biolog Phenotype Mammalian …

Soil Health On Utah's Farms And Ranches, Jenae Dean

All Graduate Theses and Dissertations, Fall 2023 to Present

The term soil health has gained popularity in agriculture due to prolonged droughts, rising food costs, and the growing public concern for sustainable and organic practices. Soil health practices can offer solutions to all of these pressures. This thesis provides Utah's farmers and ranchers with quantifiable data on implementing soil health practices in Utah's unique climate. It presents the first two years of on-farm trial results on yield and quality after implementing various soil health practices on five categories of crops: corn, mixed hay, alfalfa, produce, and dryland grain. Over a two-year period, none of the soil health practices resulted …

Group I Introns As Catalytic Rna Models To Investigate The Origin Of Life And To Explore The Drivers Of Evolution In The Rna World, Gianluca Peri

Boise State University Theses and Dissertations

The RNA World is the theory that life on Earth originated as RNA molecules able to both carry genetic information and catalyze enzymatic activities, without the need of DNA nor proteins. Abundant evidence supports that RNA preceded the emergence of DNA and proteins, the most notable being that both DNA replication and protein synthesis require RNA components. It has been hypothesized that the RNA World originated as a self-replicating RNA system. Furthermore, since the activity of modern-day catalytic RNA (ribozymes) can be affected by reaction conditions (e.g. Mg^2+ cofactor concentrations), it is hypothesized that evolution in the RNA World may …

Identification Of Anti-Inflammatory Pathways Induced By Enterotoxin B Subunits For Use As Therapeutics In Inflammatory Bowel Disease, Hannah Juel Hedelius

Boise State University Theses and Dissertations

Vibrio cholerae’s AB5 enterotoxin, cholera toxin (CT), has a homopentameric, non-toxic B subunit (CTB). CTB is a well-known vaccine adjuvant used for its ability to create a strong immune response, however more recent studies have shown its ability to also behave in an anti-inflammatory manner, performing as a protective agent in a variety of different inflammatory diseases. Escherichia coli (E. coli) produces a heat-labile AB5 enterotoxin (LT) that is very homologous to CT, with its B subunit (LTB) being used in a similar manner as a vaccine adjuvant. There have been many studies comparing LTB to CTB due …

Social And Economic Gradients Of Resource Transfer Behavior In Gorongosa, Mozambique, Karl J. Mertens

Boise State University Theses and Dissertations

Why do people who experience the same environmental and social conditions vary so much in their resource transfer behavior? This dissertation addresses that question through examination of behavioral variation in subsistence horticulturalists in the dry tropical forest bufer conservation area of Gorongosa National Park in Mozambique. In this region of moderate and acute food shortages at both household and regional levels, individuals face similar challenges of addressing social and resource uncertainty yet respond with marked behavioral variation that cannot be explained solely by diferences in wealth, kinship, or reciprocity patterns.

Using a Behavioral Reaction Norms (Dingemanse et al., 2010) (BRN) …

Sustainability: Buzz Word Or Future Of Fashion? Measuring The Feasibility Of Outright Sustainability Among Fast Fashion’S Biggest Agents, Ryan Miller

Apparel Merchandising and Product Development Undergraduate Honors Theses

Abstract

The fashion industry is currently experiencing unsustainable rates of pollution within its supply chains. The rapid increase in demand for short lead times perpetrated by large-scale retailers has led to hazardous practices negatively affecting both the environmental conditions and working conditions of producing countries. With this increased pressure, relationships between brands and suppliers have become untenable. Limited transparency and imbalanced power dynamics at the hand of the industry’s leading retailers require restructuring in order to build more sustainable and equitable supply-chain practices. Further, governmental regulation is currently limited in its capacity to enforce sustainable business practices on a global …

Coyote (Canis Latrans) And Non-Target Wildlife Responses To Livestock Guardian Dogs At Sheep Grazing Sites On The California Central Coast, Tricia T. Nguyen

Master's Theses

Livestock depredation is one of the oldest and most widespread forms of human-wildlife conflict. The coyote (Canis latrans), a midsized canid, represents a significant proportion of domestic sheep depredations in the United States, costing livestock producers an estimated 24.6 million dollars annually. Management strategies for coyote-sheep depredation vary widely in their effectiveness and in their level of public support. Identifying appropriate strategies for farms, ranches, and other sheep grazing sites remains a pressing need due to the potentially negative consequences for surrounding ecosystems and socioeconomic outcomes for sheep producers. The livestock guardian dog (LGD) is a nonlethal control …

Microbial Guardians Of The Savannah: Unraveling The Gut Microbiome For African Carnivore Conservation, Laura E. Peirson

All NMU Master's Theses

Non-invasive fecal sampling is essential for wildlife gut microbiome (GMB) research, yet field conditions often limit preservation options. I compared gut bacterial communities in paired fecal samples preserved in stabilization tubes and air-dried in paper bags collected from anesthetized African lions (Panthera leo) and spotted hyenas (Crocuta crocuta) in Etosha National Park, Namibia. Additional opportunistic samples from carnivore kill sites were also analyzed. No significant differences in alpha or beta diversity were detected between preserved and dried samples, although bacterial beta diversity differed between preserved and opportunistic samples (PERMANOVA). Core bacterial communities remained consistent across sample …

Crevice Corrosion Mechanisms Of Cocrmo Alloys In Orthopedic Implants: Retrieval Analysis, Nano-Tribocorrosion And Cellular Responses, Hwaran Lee

All Dissertations

Mechanically assisted corrosion and non-mechanically (chemically) driven corrosion in modular junctions of orthopedic implants using cobalt-chromium-molybdenum (CoCrMo) alloys remain clinical concerns and may contribute to implant failure. The underlying corrosion mechanisms are not yet fully understood, leaving a critical research gap. We hypothesized that corrosion in modular junctions may be driven by aggressive local environments, including fretting, metal ion release (cobalt ion, Co²⁺), low pH and reactive oxygen species (ROS). This dissertation aims to (1) analyze corrosion modes and potential causes in modular junctions; (2) identify chemically driven corrosion modes on CoCrMo alloys using simulated inflammatory modular taper …

Advanced Specificity And Sensitivity Studies Relative To A Research Use Only Transcription-Mediated Amplification-Based Assay For Treponema Pallidum Rna Detection, Trinity Krueger, Grace Kadonsky, Elizabeth Thelen, Amanda Zapp, Josephine Moore, Ahmet Muslu, Allan Pillay, Irene A. Stafford, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

Preliminary experimentation has suggested that a research-use-only real-time transcription-mediated amplification assay for Treponema pallidum (RUO T. pallidum TMA) yields instances of T. pallidum nucleic acid detection that coincide with non-treponemal serology in men who have sex with men (MSM) at increased risk for sexually transmitted infection. To further characterize the specificity of RUO T. pallidum TMA testing, 3,586 rectal swab specimens reported as “not detected” by the assay generated a mean endpoint FAM fluorescence of 637.5 units. Introduction of Treponema denticola, Treponema phagedenis, and Treponema refringens nucleic acid into matrices generated mean endpoint FAM fluorescence ranging from 620.7 …

Factors Underlying A Latitudinal Gradient In The S/G Lignin Monomer Ratio In Natural Poplar Variants, Jin Zhang, Pradeep Kumar Prabhakar, Yen On Chan, Rachel A. Weber, Mengjun Shu, Ganesh Panzade, Connor J. Cooper, Russell B. Davidson, Jin-Gui Chen, Sara S. Jawdy, Jerry M. Parks, Breeanna R. Urbanowicz, Gerald A. Tuskan, Trupti Joshi, Richard A. Dixon, Wellington Muchero, Jaime Barros

Biomedical Sciences

The chemical composition of wood plays a pivotal role in the adaptability and structural integrity of trees. However, few studies have investigated the environmental factors that determine lignin composition and its biological significance in plants. Here, we examined the lignin syringyl-to-guaiacyl (S/G) ratio in members of a Populus trichocarpa population sourced from their native habitat and conducted a genome wide association study to identify genes linked to lignin formation. Our results revealed many significant associations, suggesting that lignin biosynthesis is a complex polygenic trait. Additionally, we found an increase in the S/G ratio from northern to southern geographic origin of …

Analysis Of The Scleroderma / Systemic Sclerosis Etiopathology Using The University Of California At Davis (Ucd)-Ssc Chicken Line, Angeline E. Rodriguez

Graduate Theses and Dissertations

Scleroderma/Systemic Sclerosis (SSc) is a multifaceted autoimmune disorder affecting approximately 300,000 people in the United States. Main features include vasculitis, immune dysfunction (innate and adaptive), autoantibody production, perivascular leukocyte infiltration, and extensive tissue fibrosis. The University of California, Davis (UCD) SSc chicken line was shown to exhibit all clinical and biological manifestations of human SSc. One early and distinctive clinical sign in both humans and UCD-SSc chickens is Raynaud’s phenomenon (RP), characterized by decreased blood flow in extremities and skin lesions. RP-like lesions develop in combs of UCD-SSc chickens within one- to two-weeks post-hatch, progressing from erythema and edema to …

Consumer Knowledge, Attitude, And Practice For Animal-Raising And Environment-Related Labeling Claims, Andrew Joseph Dilley

Graduate Theses and Dissertations

In the fall of 2024, the Food Safety and Inspection Service (FSIS) updated its guidelines for substantiating animal-raising and environment-related claims used on meat and poultry products. The updates aim to address numerous comments and petitions from industry stakeholders by improving guidance and strengthening the documentation recommended for substantiation. While the updates included both minor and substantial revisions, the most notable addition was the strong emphasis on the inclusion of a third-party certification in the substantiating documents. The timely update comes on the heels of heightened scrutiny regarding animal-raising claims, as issues of non-compliance and agency oversight have continued to …

The Market For Local Beef And Local Beef Processing Investment In Arkansas., Rebekah O'Neal

Graduate Theses and Dissertations

The COVID-19 pandemic exposed vulnerabilities in the U.S. food supply chain, particularly in the beef sector, where processing bottlenecks and labor shortages disrupted meat production and the market. In response, several policies were implemented to strengthen food system resilience, and significant funding was allocated to expand independent meat processing capacity nationwide. However, the long-term viability of expanding regional processing capacity remains uncertain, notably in Arkansas, a net exporter of live cattle. This study evaluates whether consumer demand supports the expansion of local beef processing in Arkansas. Using a dichotomous choice survey, we estimate market shares for beef labeled “Arkansas Grown” …

Factors Associated With Farm Service Agency Direct Farm Ownership Loan Delinquency, Mohammad Ismile Hossain

Graduate Theses and Dissertations

This thesis investigates the factors associated with loan defaults in the USDA's Direct Farm Ownership (FO) loan program, emphasizing factors that affect how long it takes to default. This study uses a Cox Proportional Hazards model on a dataset of more than 47,000 loan records originated during 2011-2020 to identify loan characteristics, financial factors, and geographic settings related to the likelihood and timing of delinquency, which is defined as being 90 days past due. Recent research has focused on differences in loan outcomes based on demographics. This study, on the other hand, focuses on loan size, interest rates and terms, …

Exploring Fisheries Species Distribution Shifts In Response To Freshwater Inflow Through The Bonnet Carré Spillway Using Species Distribution Models, David T. May

Master's Theses

The Bonnet Carre Spillway (BCS) is a flood control structure on the Mississippi River (MR) ~52 km upstream of New Orleans. The BCS protects downstream communities from MR flooding by diverting floodwaters into Lake Pontchartrain when the MR exceeds 5.18m flood stage. Diverted waters flow east into the Mississippi Sound (MSS), introducing turbid, nutrient rich freshwater to the estuary before draining into the Gulf of Mexico. BCS openings have become much more frequent in the last decade, though little work has been done to explore the impact of the BCS on nektonic fisheries species. Here, a series of seasonal Maximum …

Assessment Of A Muscadine Grape (Vitis Rotundifolia) Mapping Population For Tolerance To Pierce’S Disease Pathogen Xylella Fastidiosa And Resistance To Black Rot Disease Pathogen Guignardia Bidwellii, Anushka Tennakoon

Master's Theses

Pierce’s disease (PD) and black rot disease cause severe economic losses for grape production under humid and warm conditions. Identifying resistance genes is crucial for controlling these disease by developing resistant cultivars. Muscadine grape (Vitis rotundifolia) cultivar ‘Pam’ has been identified to possess a high level of resistance to diseases. To elucidate the genetic basis of the resistance to the causal pathogens Xylella fastidiosa and Guignardia bidwellii in this cultivar, parents and 141 F1 individuals developed from open pollinated ‘Pam’ were evaluated for two seasons under natural disease pressure. The mapping population was genotyped using genotyping-by-sequencing (GBS) and …

Multicenter Span Trial Of Fasudil In Ischemic Stroke, Takahiko Imai, Andreia Lopes De Morais, Xuyan Jin, Tao Qin, Jessica Lamb, Karisma A Nagarkatti, Mu-Hsun Chen, Ligia S B Boisserand, Rakesh B Patel, Mariia Kumskova, Anjali Chauhan, Krishnan M Dhandapani, Mohammad B Khan, Pradip K Kamat, Yanrong Shi, Suyi Cao, Basavaraju G Sanganahalli, Joseph B Mandeville, Patrick D Lyden, David C Hess, Enrique C Leira, Anil K Chauhan, Jaroslaw Aronowski, Louise D Mccullough, Raymond C Koehler, Lauren H Sansing, Márcio A Diniz, Cenk Ayata

Children’s Nutrition Research Center Staff Publications

Background: The SPAN (Stroke Preclinical Assessment Network) is a confirmatory multicenter trial network to test cerebroprotective interventions in experimental acute stroke. In a first-of-its-kind trial, SPAN tested 6 interventions in a rodent model of transient focal ischemic stroke. Here, we report the efficacy of fasudil, an isoform-nonselective rho-associated kinase inhibitor, on primary and secondary outcomes in the SPAN trial.

Methods: Fasudil was administered at 10 mg/kg intraperitoneally every 12 hours for 6 doses starting 5 minutes before reperfusion in a 60-minute endovascular filament middle cerebral artery occlusion model. The active treatment arm (n=345) was compared with the pooled intraperitoneal and …