Digital Commons Network^™

Claudin-1-Mediated Signaling And Therapy Resistance In Colorectal Cancer: From Mechanism To Translation, Mark W. Primeaux

Theses & Dissertations

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, with metastatic cases showing poor response to chemotherapy due to both intrinsic and acquired therapy resistance. Claudin-1 (CLDN1), a tight junction protein, is overexpressed and mislocalized outside of tight junctions in CRC, where it contributes to an aggressive, metastatic phenotype. Although this causal relationship has been well established, the mechanisms by which CLDN1 promotes tumor progression were unclear due to its lack of intrinsic enzymatic activity. This dissertation investigates the molecular mechanisms through which CLDN1 drives oncogenic signaling, its role in therapy resistance, and its translational potential as both a …

Scientist Perspectives: A Correlation Analysis Of Translational Science Competencies Compared With Principles Of Engagement And Knowledge Integration, Rachel Wenzl

Theses & Dissertations

The competencies identified in the development of the “Fundamental Characteristics of the Translational Scientist” may not consider and fully integrate the concepts of knowledge integration and engagement, key components of the translational science spectrum. This gap may be widening and deepening the “valley of death” and exacerbating ongoing challenges in the field of translational science. This dissertation explored the association of knowledge integration, stakeholder engagement, and community engagement principles compared with translational science competencies.

Utilizing content review and a non-experimental, correlation study design, data were collected through a quantitative survey from adult research subjects all self-identified as a research investigator …

Analyzing Food Access: An Investigation Of Food Insecurity Amongst Latinos In Rural Nebraska, Natalia Santos

Theses & Dissertations

Background: Food insecurity is a public health issue in the United States, with disproportionately higher rates among Latino/Hispanic households. Rural communities face unique geographical, socioeconomic, and systemic barriers that often exacerbate food access disparities. Despite the growing Latino population in rural areas, particularly in Midwestern states like Nebraska, there remains a critical gap in comprehensive research that integrates both objective and subjective understandings of their food environments. Understanding these multifaceted influences is crucial for developing effective, culturally sensitive interventions.

Problem Statement: This dissertation aims to answer the central question: How does food access and food insecurity manifest amongst Latinos in …

Diverse Cell Types Establish A Pathogenic Immune Environment In Peripheral Neuropathy, Julie Choi

Arts & Sciences Graduate Student Theses and Dissertations

Neuroinflammation plays a complex and context-dependent role in many neurodegenerative diseases. We identified a key pathogenic function of macrophages in a mouse model of a rare human congenital neuropathy in which SARM1, the central executioner of axon degeneration, is activated by hypomorphic mutations in the axon survival factor NMNAT2. Macrophage depletion blocked and reversed neuropathic phenotypes in this sarmopathy model, revealing SARM1-dependent neuroimmune mechanisms as key drivers of disease pathogenesis. In this study, we investigated the impact of chronic subacute SARM1 activation on the peripheral nerve milieu using single cell/nucleus RNA-sequencing (sc/snRNA-seq). Our analyses reveal an expansion of immune cells …

Enhancing Sustainable Rice Production Through Organic Plus Fertilizer In Irrigated Paddy Fields, Oviyanti Mulyani, Emma Trinurani Sofyan, Ania Citraresmini, Benny Joy, Anne Yuliana Husen

Jurnal Kultivasi

Sustainable rice farming is increasingly threatened by declining soil fertility, excessive reliance on chemical fertilizers, and environmental degradation from intensive agricultural practices. There is an urgent need for innovative organic fertilizer products that combine organic materials with macro and micronutrient enhancements to restore soil health effectively. This research aimed to evaluate the effect of organic plus fertilizer (OPF) as a sustainable soil amendment to improve soil nutrient status, increase paddy productivity, and enhance overall soil health. The experiment was conducted using a Randomized Block Design (RBD) with nine treatments: one recommended OPF dose, six combinations of NPK (75 – 100%) …

Evaluation Of Color, Water Content, And Antioxidant Properties Of Wood Ear Mushroom With Nano Edible Coating, Packaging, And Storage Temperature, Aulia Afifah Rachman, Jajang Sauman Hamdani, Kusumiyati

Jurnal Kultivasi

Wood ear mushrooms are often consumed in Asia and Tropical America for their jelly-like texture, health, and freshness. However, they are easily damaged due to microbes and poor postharvest handling, which affects quality. The combination of nano edible coating, packaging, and storage temperature offers a solution to these issues. This study aims to evaluate the effect of nano edible coating, packaging, and storage temperature on the color (L*, a*, and b*), water content, and antioxidant properties (total phenolic and flavonoid) of wood ear mushrooms. The study was conducted at the Horticulture Laboratory, Faculty of Agriculture, Universitas Padjadjaran. The experimental design …

Phosphorylation Deficient Inducible Camp Early Repressor (Icer) Modulates Tumorigenesis And Survival In A Transgenic Zebrafish (Danio Rerio) Model Of Melanoma, Justin Wheelan, Melissa Rose Spigelman, Angelo Cirinelli, James Reilly, Carlos A. Molina

Department of Biology Faculty Scholarship and Creative Works

Melanoma, the most lethal form of skin cancer, is commonly associated with mutations in the BRAF gene, particularly BRAF^V600E, which drives tumor proliferation via the ERK1/2 signaling cascade. While BRAF inhibitors initially demonstrate efficacy, therapeutic resistance remains a significant challenge. Emerging evidence implicates the cAMP signaling pathway, particularly the cAMP response element-binding protein (CREB) and its repressor, inducible cAMP early repressor (ICER), in melanoma progression and drug resistance. ICER, a transcriptional repressor regulated via Ras/MAPK-mediated phosphorylation and ubiquitination, is degraded in melanoma, undermining its tumor-suppressive role. In a braf^V600E; p53 (loss of function) transgenic zebrafish ( …

Protocol For Vibratome Sectioning, Immunofluorescence, And S-Phase Labeling Of Inner Ear Organoids, Jiayi Wu, Stefan Heller, Maggie S. Matern

Biology Faculty Publications

Inner ear organoids represent a potentially inexhaustible source of otic tissues, including sensory hair cells and supporting cells, for in vitro manipulation. Here, we present a protocol for labeling S-phase entry of cells in inner ear organoids using 5-ethynyl-2′-deoxyuridine (EdU), followed by fixation and vibratome sectioning. Nuclear EdU is then detected alongside protein markers of interest via immunofluorescence. This workflow enables the visualization of cell and tissue morphologies within developing organoids and assessment of how different manipulations affect cell proliferation.

Proteomics-Based Aging Clocks In Midlife Or Late-Life And Their Associated Risk Of Dementia, Sanaz Sedaghat, Saeun Park, Rob F Walker, Shuo Wang, Jialing Liu, Timothy M Hughes, Behnam Sabayan, Weihong Tang, Josef Coresh, James S Pankow, Keenan A Walker, Ramon Casanova, Ruth Dubin, Rajat Deo, Jerome I Rotter, Alexis C Wood, Peter Ganz, Pamela L Lutsey, Weihua Guan, Anna Prizment

Children’s Nutrition Research Center Staff Publications

Background: Biological age can be quantified by composite proteomic scores, called proteomics-based aging clocks (PACs). We investigated whether a discrepancy between chronological and biological age in midlife and late-life is associated with cognition and dementia risk.

Methods: We used two longitudinal population-based studies: the Atherosclerosis Risk in Communities (ARIC) Study and the Multi-Ethnic Study of Atherosclerosis (MESA). PACs were created in ARIC at midlife (mean age: 58 years, 57% female, n = 11,758) and late-life (mean age: 77 years, 56% female, n = 4934) using elastic net regression models in two-thirds of dementia-free participants and validated in the remaining one-third …

The Efficacy Of Rosemary Oil And Microencapsulated Essential Oils On Zeuxapta Seriolae Mitigation In Yellowtail Kingfish, Seriola Lalandi, Md Reaz Chaklader, Lindsey Woolley, Ross Woodward-Williams, Richard Smullen, Gavin J. Partridge

Fisheries Research Articles

Two additives, rosemary oil and a commercial herbal product (HP; AROTEC-G) containing garlic essential oil, carvacrol and thymol were investigated for their potential to reduce the parasitic burden in yellowtail kingfish (YTK; Seriola lalandi) infected with Zeuxapta seriolae. Naturally infected fish were fed diets supplemented with either 2.5 mL.kg−1 rosemary oil or two concentrations of HP (5 and 10 g.kg−1) for 20 days. At 10 and 20 days, the effect of the diets on parasite abundance was evaluated. Feed intake and growth were similar across treatments (p > 0.05). By day 10, both rosemary oil and HP significantly …

Male Mating Preference For Larger Females Does Not Vary Among Age Classes In The Long-Lived Beetle Bolitotherus Cornutus, G. M. Jiron, C. A. Greene, E. D. Brodie Iii, Vincent A. Formica

Biology Faculty Works

Theory and past experimental work suggest that as males age, the strength of their mate preference should decrease. However, the empirical work investigating this question has primarily been conducted in insects that have very short life spans and often live for just a single mating season. This leaves a gap in our understanding of the relationship between male mate preference and age across taxa, as age can conflate with other ecological changes in a single mating season. In this study, we ask how the strength of preference for large female body size changes as males age in a long-lived insect, …

Phenotypic Evaluation Of F10 Soybean Generations From Grobogan X Slamet Cross For Large Seed Size Selection, Ponendi Hidayat, Nurtiati, Zulfa Ulinnuha, Adellansyah

Jurnal Kultivasi

Soybeans are an important crop with high nutritional value and diverse uses. Current consumer and industry preference is for soybean varieties with large seeds and high productivity. To meet these demands, one effective approach is through hybridization. This study aims to evaluate the phenotype of F10 soybean line from a cross between Grobogan and Slamet for large-seeded and high-yield. The study was conducted in the screenhouse and Plant Breeding and Biotechnology Laboratory, Faculty of Agriculture, Jenderal Soedirman University. The experiment used a Randomized Complete Block Design (RCBD) with three replications, testing genotypes from Slamet x Grobogan crosses and three check …

Effect Of Eco-Enzyme Application On Soil Nutrient And Plant Productivity Of Green Mustard-Peanut In Inceptisol, Idris Abd Rachman, Buhari Umasugi, Krishna Aji, Nurul Fahira A Hakim, Adnan Sofyan, Asrul Dedy Ali Hasan

Jurnal Kultivasi

Appropriate land management has a long-term impact on soil performance and is believed to improve soil fertility. This study investigated the effect of eco-enzymes on soil nutrients and plant productivity of green mustard-peanut in inceptisol. The research was conducted from February to April 2025. The experiment used a randomized block design (RBD) consisting of 5 treatments and replicated 3 times, so that there were 15 units, including N0 = without Eco-enzyme, N1 = Eco-enzyme 2cc/L. N2= Eco-enzyme 4cc/L, N3= Eco-enzyme 6cc/L, and N4= Eco-enzyme 8cc/L. The commodities used were green mustard (Brassica juncea L) and peanut (Arachis hypogaea Linn). Soil …

Erratum To “Nitrate And Nitrite Le Ate And Nitrite Levels In Cattle F Els In Cattle Feed, Drinking Water Eed, Drinking Water, Rumen , Rumen Content, And Blood Methemoglobin Le Content, And Blood Methemoglobin Levels In Bur Els In Burdur Province Of Vince Of Türkiye”[Turkish Journal Of Veterinary And Animal Sciences 49 (3) 2025 94-101], Reyda Kiyici, Hasan Altan Akkan, Mehmet Karaca, Esra Zeybek, Bayram Kudret Karaayvaz, Fi̇rdevs Mor Savaş, Yahya Öztürk, Asim Kart, Ramazan Yildiz, Murat Bayezi̇t

Turkish Journal of Veterinary & Animal Sciences

We hereby issue this erratum to address a concern regarding Tables 6, 7, and 8 in the manuscript titled “Nitrate and nitrite levels in cattle feed, drinking water, rumen content, and blood methemoglobin levels in Burdur Province of Türkiye”. Some of the numbers in Tables 6, 7, and 8 were misprinted by mistake during the typesetting process. To ensure the accuracy of the published work, the correct tables are included for your reference.

The original article can be accessed at: https://doi.org/10.55730/1300-0128.4376

Large-Scale Crispr Screening In Primary Human 3d Gastric Organoids Enables Comprehensive Dissection Of Gene-Drug Interactions, Yuan-Hung Lo, Hudson T Horn, Mo-Fan Huang, Wei-Chieh Yu, Chia-Mei Young, Qing Liu, Madeline Tomaske, Martina Towers, Antonia Dominguez, Michael C Bassik, Dung-Fang Lee, Lei S Qi, Jonathan S Weissman, Jin Chen, Calvin J Kuo

Faculty, Staff and Student Publications

Understanding how genes influence drug responses is critical for advancing personalized cancer treatments. However, identifying these gene-drug interactions in a physiologically relevant human system remains a challenge, as it requires a model that reflects the complexity and heterogeneity among individuals. Here we show that large-scale CRISPR-based genetic screens, including knockout, interference (CRISPRi), activation (CRISPRa), and single-cell approaches, can be applied in primary human 3D gastric organoids to systematically identify genes that affect sensitivity to cisplatin. Our screens uncover genes that modulate cisplatin response. By combining CRISPR perturbations with single-cell transcriptomics, we resolve how genetic alterations interact with cisplatin at the …

Multidimensional Analysis Of B7 Homolog 3 Rna Expression In Small Cell Lung Cancer Molecular Subtypes, Carl M Gay, Taofeek K Owonikoko, Lauren A Byers, Noura J Choudhury, Sajid Ahmed, Zachary Cain, Xiaozhong Qian, Matthew Brentnall, Simon Heeke, Ming Poi, Sharon Wu, Charles M Rudin

Faculty, Staff and Student Publications

Purpose: B7 homolog 3 (B7-H3) is a promising target for antibody-drug conjugates, with ifinatamab deruxtecan demonstrating an objective response rate of 54.8% in previously treated extensive-stage small cell lung cancer (SCLC). This analysis aimed to characterize B7-H3 RNA expression with reference to SCLC molecular subtypes (SCLC-A, SCLC-N, SCLC-P, and SCLC-I) and immune-related parameters.

Experimental design: Tumor RNA expression and mutational burden for 1,721 patients with SCLC were derived from a real-world database (Caris Life Sciences). A predominant molecular subtype was assigned based on RNA expression using a gene-ratio classifier. PD-L1 expression was assessed by IHC (antibody 22C3; positive cutoff: tumor …

Genome Sequences Of Eight Fusobacterium Watanabei Sp Isolates, Martha A Zepeda-Rivera, Falk Ponath, Kaitlyn N Lewis, Rutika P Gavate, Floyd E Dewhirst, Junko Tomida, Yoshiaki Kawamura, Kaori Tanaka, Susan Bullman, Christopher D Johnston

Faculty, Staff and Student Publications

We report draft genome sequences of eight Fusobacterium watanabei clinical isolates, ranging from 1.95 to 2.09 Mbp. Analysis against the Genome Taxonomy Database indicates that F. watanabei genomes are part of the "Fusobacterium nucleatum_J" group.

Iterative Intraoperative 3t Mri (Imri)-Guided Brachytherapy: A Prospective Study On Enhancing Implantation Precision And Dosimetric Gains In Advanced Gynecologic Cancers, Shrikiriti S Rajan, Matthew S Ning, Megan Jacobson, Samantha J Simiele, Teresa Bruno, Ramez Kouzy, Kyoko Yoshida-Court, Tatiana Cisneros-Napravnik, Henry Yu, Jason Stafford, Yusung Kim, Geena Mathew, Rauda Alicia Cordova, Maliah Domingo, Anuja Jhingran, Lilie L Lin, Melissa Joyner, Travis T Sims, Lauren Colbert, Aradhana M Venkatesan, Ann Klopp

Faculty, Staff and Student Publications

Purpose: To report on primary outcomes and dosimetric results of a prospective clinical trial and protocol for use of iterative intraoperative magnetic resonance imaging (iMRI) in gynecologic brachytherapy.

Methods: Patients with locally advanced cervical or vaginal cancer (FIGO stages IB2 - IVA, and stage II-IVA, respectively) undergoing pulsed dose rate (PDR) brachytherapy were enrolled in a prospective clinical trial (NCT03634267) using iterative 3T iMRI during brachytherapy implant placement. Applicator and optional interstitial needles were placed under iMRI guidance in a 3T clinical MRI scanner. Imaging, dosimetry and clinical outcomes (local control (LC), recurrence-free survival (RFS), overall survival (OS)), …

An Investigation Of The Association Of Gdf9-C.260g>A Polymorphism With Lamb Live Weight In The Akkaraman Breed, Şeyma Kökkaya, Fadi̇me Daldaban, Davut Bayram, Mehmet Ulaş Çinar, Korhan Arslan, Bi̇lal Akyüz

Turkish Journal of Veterinary & Animal Sciences

In this study, the relationship between the Growth Differentiation Factor-9 (GDF9) gene polymorphism and birth weight, as well as weights on days 30, 60, 90, was investigated in Akkaraman lambs, one of the indigenous sheep breeds of Türkiye. For this purpose, a total of 314 lambs were genotyped by PCR-RFLP method for the single nucleotide polymorphism (SNP) c.260G>A in the GDF9 gene. While GG and GA genotypes were observed in the studied breed, the AA genotype was not observed. The results showed that Akkaraman lambs were in Hardy–Weinberg (H–W) equilibrium for the GDF9-c.260G>A polymorphism (p < 0.05). In this study, a significant association was observed between GDF9-HhaI polymorphism and 30th day weight and 60th day weight in Akkaraman lambs. When the animals were analyzed according to birth type, it was observed that this factor was associated with birth weight, as well as weights on days 30, 60, and 90. It is suggested that the c.260G>A coded SNP …

Bacteria Recovered From Ear Swab Samples Of Cats With Otitis Externa And Their Antimicrobial Resistance Profiles In Kayseri, Türkiye, Emre Karakaya, Doğancan Yarim, Özgür Güran, Cansu Güran, Umut Alpman, İzzet Burçi̇n Saticioğlu, Gülteki̇n Atalan, Seçi̇l Abay, Fuat Aydin

Turkish Journal of Veterinary & Animal Sciences

This study aimed to isolate and identify bacteria from ear swab samples from cats with otitis externa and to detect the antimicrobial resistance profiles of the recovered isolates. For this purpose, ear swab samples from 100 domestic cats that were detected to have otitis externa were used. The samples collected from the animals were inoculated onto 7% defibrinated sheep blood agar and MacConkey agar, and the media were incubated at 37°C for 24-72h under aerobic/microaerobic conditions. The definition of the isolates was carried out by phenotypic tests, MALDI-TOF MS analysis, and 16S rRNA gene sequencing. Antimicrobial susceptibilities of the obtained …

Growth Curve And Serum Calcium And Magnesium Concentrations In Duck Breeders (Anas Boschas) From Hatching To The Prelaying Period Reared Under Subtropical Conditions, Vanessa Peripolli, Fabiano Dahlke, Ellen Karen Dos Santos, Maila Palmeira, Stelamaris Dezen, Robert Lenock, Elizabeth Schwegler, Juahil Martins De Oliveira Júnior, Ivan Bianchi, Paula Gabriela Da Silva Pires, Betina Raquel Cunha Dos Santos, Andressa Fernanda Campos, Fabiana Moreira

Turkish Journal of Veterinary & Animal Sciences

Optimizing duck production requires understanding growth variability and monitoring serum calcium and magnesium levelsas health indicators. This study aimed to analyze the growth curve, mortality, and serum calcium (Ca) and magnesium (Mg) levels in White Mallard ducks from hatching to the prelaying period in subtropical conditions. A total of 1100 females and 270 males were raised for 146 days. At hatch, day 6 (D6), D13, D23, D34, D50, D64, D80, D94, D111, and D146, ducks were weighed separately to assess body weight (BW). Blood samples were collected at hatch, D50, D94, and D146 to determine serum calcium and magnesium levels. …

A Morphological Study On Acanthocephalan Species From Wild Birds In Northern Türkiye, Mehmet Öztürk, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

The phylum Acanthocephala contains approximately 1300 acanthocephalan species. So far, only nine acanthocephalan species of wild birds have been reported in Türkiye. To study the context of acanthocephalan diversity in wild birds in Türkiye, we examined 193 birds from 69 species belonging to 16 orders. Acanthocephalans were found in seven bird species: Centrorhynchus aluconis in Buteo buteo and Buteo rufinus, Centrorhynchus spinosus in Buteo buteo and Strix aluco, Mediorhynchus papillosus in Iduna icterina, Mediorhynchus tenuis in Luscinia svecica, Plagiorhynchus cylindraceus in Erithacus rubecula and Turdus merula, Polymorphus contortus in Anas platyrhynchos, and Sphaerirostris turdi in Turdus merula. Except for Plagiorhynchus …

The Morphologic Structure Of Some Wing Muscles In The Common Pheasant (Phasianus Colchicus), Beste Demi̇rci̇, Murat Erdem Gülti̇ken

Turkish Journal of Veterinary & Animal Sciences

Avians use significant amounts of energy during flight that is supplied by muscles. The Common Pheasant, a member of the Phasianidae family, can fly swiftly over short distances and flies to evade predators. Pheasant species are terrestrial and have a global distribution. This investigation aimed to analyze the anatomical structure of skeletal muscles in pheasants. A cohort of 20 adult pheasants consisting of an equal number of females and males (10 each) was used. Dissections were performed on 5 females and 5 male pheasants for macroscopic evaluation. The remaining samples underwent immunohistochemical staining using an antimyoglobin antibody to measure the …

Screen Time And Objectively Measured Sleep Of U.S. College Students: A Brief Report, Donya Sedaghat, Eunkyung Lee, Jeanette Garcia, Keith Brazendale

International Journal of Exercise Science

International Journal of Exercise Science 18(4): 747-756, 2025. The objective was to investigate screen time (ST) and objectively measured sleep quality in college students. Participants were undergraduate students attending a large metropolitan public university in the southeastern U.S. Participants wore accelerometers on their wrist for 24hr/day for 14 days to capture sleep outcomes, and completed a survey reporting ST duration. Analyses explored weight-status, race/ethnicity, and sleep patterns (presence of daytime sleep) differences among students. Participants (n=29, 86% female, ~21.5 years old, 38% White, 35% Overweight/Obese) slept for an average of 433.8 minutes/night, reported 419.1 minutes of daily ST, and 112 …

Effects Of Light Wavelength On Broiler Performance, Blood Cell Profiles, Stress Levels, And Tibiotarsi Morphology, Angela Perretti, Victor J. Oyeniran, Jaelen M. Cherry, Rosemarry H. Whittle, Zachary Grider, Alexander H. Nelson, Seong W. Kang, Gisela F. Erf, Shawna L. Wiemer

Poultry Science Faculty Publications and Presentations

Lighting influences broiler production, health, and behavior. The objective of this study was to examine the effects of three light wavelengths (White [350-780 nm], Blue [450 nm], and Green [560 nm]) on broiler production, activity, fear, stress, blood cell profiles, and tibiotarsi (tibia) morphology. Day-of-hatch male broiler chicks (N = 600) were housed in pens (N = 12) with one lighting treatment for 42 days. Body weight and feed consumption were recorded on day (D) 0, 14, 28, and 42, and the feed conversion ratio was calculated. The Tonic Immobility test was used to assess the latency (seconds) to right …

Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu

Faculty Publications

No abstract provided.

Determining The Origin Of An Isolated Great Lakes Plant Species, Agoseris Glauca (Asteraceae), Seth Paul Hiers

Masters Theses

Small, isolated populations often have low genetic diversity and low potential for adaptation in response to environmental changes. Alternatively, isolated communities are often more likely to display local adaptations and over time speciate. The Great Lakes Region is home to over 50 vascular plant species that are disjunct from the species’ core populations. Agoseris glauca (Asteraceae) is a Great Lakes disjunct and Michigan state threatened species geographically isolated from its core range by 900 km. This study uses Genotyping by Sequencing to assess the population genetics of A. glauca for two Michigan disjunct populations and five western populations in the …

Stabilized And Unstabilized Sampling Methods Result In Differential Fecal 16s Rrna Microbial Sequencing Results, Christopher E. Stamper, Andrew J. Hoisington, Joseph C. Ellis, Christopher A. Lowry, Lisa A. Brenner

Faculty Publications

Over the past decade, studies have been conducted to increase the understanding of associations between the fecal microbiome and human health. In conjunction, researchers have investigated the effects of study design, methods, molecular processing, and sequencing techniques. However, a lack of standardization of fecal sample collection methodology has introduced heterogeneity in sequencing results. Sources of variability include sample collection methods, storage temperatures, and transport times. Here we present 16S rRNA gene amplicon sequencing results from two sample collection methods (unstabilized sterile swab and stabilized OmniGene Gut Kits) collected from the same fecal specimens. The paired samples were collected either at …

Four Nebraska Public Power District (Nppd) Irrigation Electricity Purchase Options, Matt Stockton

Cornhusker Economics

Farm managers are destined to face a myriad of choices while looking for and seeking the right balance between methods and outcomes that satisfy their individual farm’s needs. Successful farms require productivity, profitability, and continuity. This holds true for the many production choices made throughout the year. One of these opportunities is the purchase of electricity for irrigation. Nebraska is a unique state in that its electric power is generated and delivered by public power companies. The largest single entity in that system is NPPD, which serves all or part of 84 of the 93 counties within the state. NPPD …

The Lesson Horse: A Guide For Traditional Horsemanship & Equine-Assisted Services Programs 2nd Edition, Sarah Andersen

Animal, Dairy & Veterinary Sciences Textbooks

This practical guide equips equine professionals with the tools to select, assess, and develop safe, capable horses for traditional horsemanship and equine-assisted services programs. It introduces structured assessment tools to evaluate horse suitability and performance, supports the creation of individualized training plans, and addresses long-term horse welfare through continued evaluation and thoughtful end-of-work planning.