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Comparative Assessment Of Lower Limb Neuromusculoskeletal Activation And Joint Kinematics In Normal And Impaired Gait, Jack Ryan Schule

Theses and Dissertations

This thesis evaluates whether accessible and affordable technologies can provide clinically significant, quantifiable gait analysis comparable to traditional marker-based motion capture systems. Modern high-end systems offer excellent accuracy but remain financially and logistically inaccessible to many clinicians and researchers. To address this gap, a framework was developed using markerless pose estimation using the model, MediaPipe, inertial measurement units (IMUs) processed through an Extended Kalman Filter (EKF), and surface electromyography (sEMG) to measure joint kinematics and neuromuscular activity during gait. An experiment was performed where data was collected from participants with both normal gait and pathologies including: Osgood–Schlatter disease, Plica Band …

An Investigation Of The Characteristics And Uses Of School Farms In California Ffa’S Superior Region, Emily R. Fuller, Jessica M. Toombs

Journal of Research in Technical Careers

This study investigates the characteristics and uses of school farms within Northern California, focusing on their role in school-based agricultural education (SBAE). The research aims to determine the structural and operational attributes of these facilities and to identify their primary uses as experiential learning tools. Utilizing a descriptive survey design, data were collected from 74 agricultural educators. Findings reveal that school farms, averaging 21.27 acres, are predominantly managed by agriculture teachers and are primarily used for housing market animals, supporting Supervised Agricultural Experience (SAE) projects. The study highlights the need for diversified school farm facilities to enhance experiential learning across …

Associations Between Food Insecurity And Gpa Among Full-Time California State University Undergraduates During Covid-19: The Impact Of Mental Health, Lindsey Jones

Master's Theses

Food insecurity is a persistent public health concern among college students that has been associated with student’s academic and mental health outcomes. This thesis examined the associations between food insecurity, academic performance (measured by GPA) and mental health (using Kessler 6 psychological distress and self-perceived mental health impacts on academic performance), among full-time undergraduate students within the California State University (CSU) system. Cross-sectional analyses were conducted using data from the Spring 2021 CSU National College Health Assessment, administered across all 23 campuses. Of the 20,167 respondents, 12,899 students were included in the analytical sample. Bivariate analyses were conducted to analyze …

Phenology And Seed Treatment Impacts Surface Defenses And Fall Armyworm (Spodoptera Frugiperda) Growth In Rice (Oryza Sativa), Devi Balakrishnan, Jessica Ayala, Alejandro Vasquez, Nick Bateman, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

Rice (Oryza sativa L.) is considered as the most important staple food crop in the world, feeding half of the global population. One of the emerging concerns in rice is the fall armyworm (Spodoptera frugiperda, FAW), a highly polyphagous insect herbivore. Although FAW has been well studied across many host systems, rice-FAW interactions-including the effects of phenology and seed treatment-are less understood. In this study, we examined the response of FAW to rice under a thiamethoxam seed treatment across three different phenological stages (V5: tillering; V11: maximum tillering; R3: panicle exsertion). We used four commonly grown cultivars in USA, exposed …

Context Dependent Regulation Of Dicer Function During Oocyte-To-Embryo Transition, Nick A. Newkirk

Dissertations and Theses (Open Access)

Over two decades ago, Dicer was discovered as the primary enzyme responsible for the generation of small RNAs known as the microRNAs (miRNAs) and short interfering RNAs (siRNAs). Spatiotemporal regulation of Dicer-dependent small-RNA biogenesis affects many aspects of biology, including aging, disease progression, fertility, and cancer. Interestingly, a distinct population of Dicer-dependent small RNAs, known as the endogenous siRNAs (endo-siRNAs), are uniquely abundant in the germ cells and gametes, and are critical for normal fertility. Despite their function regulating new life, the mechanisms controlling their biogenesis and subsequent germ cell functions remain poorly defined. Work to parse these mechanisms in …

Capsinoids, Non-Pungent Trpv1 Agonists, Induce A Mild Hypothermia That Provides Short- And Long-Term Neuroprotection After Ischemic Stroke, Alexander Andersohn, Alexander Andersohn

Dissertations and Theses (Open Access)

Therapeutic hypothermia (TH) has demonstrated neuroprotection in instances of cardiac arrest and neonatal hypoxia/ischemia but faces different challenges in application to stroke due to the activation of cold defense mechanisms in conscious patients. This dissertation examines the efficacy and specificity of capsinoids (a purified mixture of capsiate and dihydrocapsiate) to induce a sustained mild hypothermia in conscious mice that is neuroprotective after stroke. Capsinoids function as TRPV1 agonists. However, unlike capsaicin, capsinoids are vulnerable to esterase-mediated breakdown, thus significantly restricting their action to the site of delivery. We showed that capsinoids delivered intraperitoneally (IP) to mice induced a TRPV1-dependent drop …

Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins

Dissertations and Theses (Open Access)

Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.

The FtsA^{M96E R153D} variant, which has …

Neural Dynamics Supporting Flexible Cognition, Meredith J. Mccarty

Dissertations and Theses (Open Access)

Flexible cognition enables humans to adapt their behavior to changing circumstances by prioritizing relevant information and filtering out distractions. This process is thought to be supported by the integration of ongoing behavioral goals with incoming sensory information, but how this integration is realized in the human brain remains unclear. It is unknown how high-order sensory cortices can balance specialized processing with flexible integration of changing behavioral goals. In the following investigations, we use intracranial EEG (iEEG) recordings in humans to study the neural dynamics of flexible cognition during high-order sensory processing. First, we validated assumptions about the locality of iEEG …

Orthogonal Comparison Of Nuclear And Mitochondrial Clonal Architectures In Hematologic Malignancies, Nehali Shah

Dissertations and Theses (Open Access)

Acute myeloid leukemia (AML) is a hematologic malignancy characterized by accumulation of mutations that disrupt hematopoietic differentiation and promote clonal expansion. Understanding how these mutations arise and evolve is essential for improving diagnosis, prognosis, and treatment stratification. Current methods are limited by either restricted genomic coverage (targeted panels) or low throughput and high cost (in single-cell whole genome sequencing, scWGS). An emerging alternative is the use of mitochondrial DNA (mtDNA) mutations as clonal markers.

This study aims to determine whether mitochondrial-derived clonal architectures correlate with nuclear-derived clonal architectures in AML, thereby evaluating mtDNA as a scalable orthogonal tool for lineage …

A Mixed Methods Study Of The Weather And Climate Science Curriculum Pilot In South Carolina K-12 Schools, 4-H, And Agricultural Education Programs, Emily B. Hood

All Dissertations

Weather and climate science topics in K-12 education are more important now than ever due to extreme weather and climate events happening worldwide. Most U.S. science teachers included climate science in their courses, but they may not have a sufficient background in the topic which can hinder their efforts, or there is no explicit curriculum for the teacher to utilize. Research indicates that there is a need for innovation in the curriculum to help prepare children to become the next generation of leaders and address complex problems, such as issues related to weather and climate science. There are a few …

Horticultural Strategies To Improve Yield And Efficiency For Controlled-Environment Cannabis Production, Michael John Alden

All Dissertations

Controlled-environment cannabis (Cannabis sativa L.) production is a novel horticultural industry due to long-standing federal prohibitions associated with production, distribution, and consumption of this species. Many cannabis production methods are based on legacy knowledge and hypotheses that often contradict standard practices used in the agriculture and floriculture sectors. During this research, several horticultural practices were evaluated, including shoot number manipulation, fertilizer restriction, harvest timing, and photoperiod manipulation. Shoot number manipulation was achieved by repeatedly pinching apical shoots, which reduced reproductive shoot length (cm/shoot) and improved inflorescence:trim ratio, both of which can reduce material cost in production and labor cost …

Breeding For Disease Resistance In Melon And Watermelon, Venkata Rao Ganaparthi

All Dissertations

Watermelon and cantaloupe are two widely consumed cucurbits, but their acreage in the United States reduced by nearly 50% in last two decades. Fusarium wilt caused by a soil borne pathogen Fusarium oxysporum f. sp. niveum(Fon) race 2 is a major limiting factor in watermelon production as it reduces yields by 80%. Grafting watermelon plants onto the root stock of wild, disease-resistant varieties like ‘Carolina Strongback’ is the best strategy to manage Fon race 2. Though effective, this approach increases production costs.

Part of this dissertation focused on finding more sustainable, cost-effective solutions to control Fusarium wilt …

Strategic Enhancement Of Biofuel Production Using Bacillus Subtilis As A Model Organism: A Dual Approach Of Gene Essentiality Mapping And Optimization, Angela A. Zebede

Electronic Theses and Dissertations

With rising global demand for renewable energy, enhancing microbial platforms for biofuel production is vital. This study engineered Bacillus subtilis to improve resilience to solvent stress by targeting membrane lipid biosynthesis pathways essential for integrity. Growth curve analysis (OD₆₀₀) showed that phosphatidylserine (PS) and phosphatidylethanolamine (PE) knockout strains grew significantly slower than wild type (168), indicating the importance of these lipids under stress. Laurdan fluorescence spectroscopy was used to assess membrane properties. Generalized polarization (GP) analysis revealed a concentration-dependent reduction in membrane order upon 1%–2% 1-butanol exposure. GP values declined progressively from control to 2% treatment, reflecting increased membrane fluidity …

Mountain Communities During A Time Of War: A Case Study Of The Hutsul Region In The Carpathian Mountains Of Ukraine, Pavlo Rybaruk

Electronic Theses and Dissertations

This thesis investigates the ethno-ecological technologies employed by the Hutsul people within the highland agrarian economy of the Carpathian Mountains. Over centuries, the Hutsuls have developed ethno-ecological technologies that maintain ecological integrity while supporting community livelihoods.

The study addresses three primary research questions: What are the most important ethno-ecological technologies of the Hutsul highland economy? How might these traditional ethno-ecological practices be applied to economic, agricultural, and tourism development in the Hutsul region? How might these traditional ethno-ecological practices be combined with contemporary approaches to encourage sustainable development in the region?

The research explores the devastating impact of the Russian …

Testing Microscopic Approaches For Cementochronology In The 21st Century Identified Skeletal Collection, Portugal, Amando Luis Montalvo Jr.

Theses and Dissertations

Age-at-death estimation is a key component to the creation of a biological profile in forensic anthropology. Acellular extrinsic fiber cementum annulations have been shown to correlate with chronological age. Cementochronology examines this correlation through the sum of the alternating translucent bands and opaque annuli, and the age of eruption of a tooth. While several microscopy techniques have been used, there is no consensus on the most optimal. This study will assess the reliability, precision and accuracy of three microscopy techniques: Brightfield (BF), Phase Contrast (PC) and Differential Interference Contrast (DIC). PC was the most reliable (rTEM= 10.4%), the most precise …

Taxonomy Of Endophytic Fungi Associated With Vallisneria Neotropicalis, Md. Arafat Rashid

Graduate Theses and Dissertations (2019 - present)

This study presents the first comprehensive taxonomic and ecological investigation of endophytic fungi (EF) associated with the submerged aquatic macrophyte Vallisneria neotropicalis in the southern United States. Over a 12 month period, from April 2023 to March 2024, leaf samples were collected from two distinct sites in Mobile Bay, Alabama a disturbed, brackish Causeway location and a cleaner, less impacted site at Meaher State Park. Using culture dependent methods, a total of 257 fungal endophytes were isolated from 1,200 leaf segments. All isolates belonged to the phylum Ascomycota, distributed across 3 classes, 6 orders, 10 families, and 19 taxa. The …

Improved Model For Neurodegeneration In C. Elegans, Jean-Pierre Arditi

Graduate Theses and Dissertations (2019 - present)

During Alzheimer's disease (AD), insoluble amyloid beta (AP) peptides accumulate to form extracellular aggregates (plaques). The direct cause of neuronal dysfunction observed in AD has been broadly investigated. The amyloid hypothesis states that AP plaques are neurotoxic, but recent studies support the AP oligomer hypothesis, which states that intracellular AP (iAP) is neurotoxic. To test this hypothesis, I used CRISPR-Cas9 gene editing to generate two transgenic Caenorhabditis elegans (C. elegans) strains. I generated a strain (RSLl 11) using the rab-3 promoter to co-express GFP in neurons, which showed no gross behavioral changes but had a 50% reduced egglaying rate. RSLl …

A Molecular Mechanism Of Epithelial To Mesenchymal Transition (Emt): An Evidence Based Lesson Applying Molecular Biology Through The Lens Of Cancer, Sophie Hasson, Melissa Rowland-Goldsmith

Open Educational Resources

This lesson aims to strengthen students’ critical thinking and molecular biology data analysis skills as well as their understanding of concepts related to the hallmark of cancer: tissue invasion and metastasis. Students begin by learning about the invasion of cancer cells, Epithelial-Mesenchymal Transition (EMT), and an important protein involved in EMT: E-cadherin. They then apply molecular biology knowledge to analyze data from multiple papers to infer the relationship between Snail and E-cadherin in different types of cancers. Next, they continue to interpret data from many papers to determine that a repressor transcription factor is present during EMT in cancer cells …

Twim #222: Biosensors In Bacteria, Madison Hayes, Alexis Austin, Dillon Nguyen, Angela Wilson, Rebecca Seipelt-Thiemann

School of Medical Diagnostics & Translational Sciences Publications

Podcast annotation TWiM #222: Biosensors in Bacteria from the weekly podcast series "This Week in Microbiology" (TWiM), a podcast where experts in microbiology discuss academic papers in their field in an informal way.

Twim #243: Beef And Bacillus, Sakiem Winston, Madison Wolford, Rebecca Seipelt-Thiemann, Angela Wilson

School of Medical Diagnostics & Translational Sciences Publications

Podcast annotation TWiM #243: Beef and Bacillus from the weekly podcast series "This Week in Microbiology" (TWiM), a podcast where experts in microbiology discuss academic papers in their field in an informal way.

Twim #286: Integrons And Invasion, Andrea Ayala-Lopez, Brittney Heist,, Thomas Salazar, Rebecca Seipelt-Thiemann, Mel Melendrez-Vallard, Angela Wilson

School of Medical Diagnostics & Translational Sciences Publications

Podcast annotation TWiM #286: Integrons and Invasion from the weekly podcast series "This Week in Microbiology" (TWiM), a podcast where experts in microbiology discuss academic papers in their field in an informal way.

Birds In Changing Landscapes: Community Diversity And Breeding Phenology, Lindsay Peria

Master's Theses

Humans are altering the environment in varied and widespread ways. Increasing urban and agricultural development causes changes to habitat and resource availability as well as changes to the sensory environment via light and noise pollution. Climate change also causes more extreme temperature anomalies, storms, and droughts that challenge birds in a multitude of ways. In this thesis, we assessed these impacts of these changes on a local and continental scale. In the first chapter, we assessed the impact of natural, anthropic, and structural characteristics of viticultural land on both taxonomic and structural avian diversity. Loss of habitat from agriculture is …

Associations Between Feed Efficiency And Feeding Behavior In Yearling Angus Bulls In A Competitive Environment, Amelia Katz

Master's Theses

Feed efficiency in beef cattle is a complex trait shaped by physiological, behavioral, and social factors that influence how animals access and utilize feed resources. This study examined feed efficiency classification and how it is influenced by feeding behavior, competitive interactions, and bunk approach patterns in yearling Angus bulls housed in a drylot over two years. Bulls were grouped into high and low feed efficiency categories using residual feed intake and feed conversion ratio within pen and year (n = 40 per year; n = 40 per treatment). Continuous electronic monitoring quantified feeder visitation, feeding duration, intake, and rate. A …

Serum Concentrations Of Inflammatory Markers And Selected Nutrient Metabolites In Holstein And Jersey Cows Across The Transition Period, Avery Orr

Master's Theses

The transition period, spanning the weeks surrounding parturition, requires dairy cows to undergo synchronized metabolic, endocrine, and immunological adjustments that support the onset of lactation but also heighten disease susceptibility. Although individual inflammatory or metabolic biomarkers have been extensively studied, the integrated immunometabolic dynamics that occur across this period and the difference between breeds is not fully understood. The objective of this study was to characterize longitudinal patterns in key nutrient metabolites and inflammatory markers in Holstein and Jersey cows and assess how these systems interact across the transition period.

A total of 63 Holstein and Jersey cows were sampled …

Testing For Local Adaptation In Sympatric Monkeyflowers Growing In Serpentine Seeps And Adjacent Rock Outcrops, Avin Niknafs

Master's Theses

Divergent selection across heterogeneous environments can drive ecological differentiation and the evolution of locally adapted populations, even when gene flow occurs. We tested for local adaptation between two sympatric monkeyflowers, Erythranthe guttata and E. serpentinicola (formerly Mimulus), which co-occur on the central coast of California. Erythranthe guttata is a perennial species primarily found in moist serpentine seeps, whereas E. serpentinicola is an annual species restricted to adjacent serpentine rock outcrops. Using a reciprocal transplant experiment with second-generation, greenhouse-derived seed, we quantified germination, survival, and reproductive success across contrasting microhabitats. We further evaluated the relative contributions of species, lineage, …

Robustness Of Network Inference Algorithms Under Network Class Misspecification, Roberto Ceja

Electronic Theses, Projects, and Dissertations

Inferring phylogenies in the presence of hybridization remains a difficult problem. As a result, many current methods for reconstructing phylogenetic networks are restricted to a simple class of networks known as level-1. This restriction arises from theoretical considerations rather than empirical evidence, with real data possibly arising from complex networks. In this work, we evaluate the robustness of two level-1 network inference methods, SNaQ and NANUQ+, through a simulation study, when the input data originates from a more complex network. Specifically, we investigate whether these methods can accurately recover important features of the true species network, such as the circular …

Requirement Of Prepiilin Peptidase Activity For Variovorax Paradoxus Eps Flp/Fap Pilus Assembly, Jenny L. Lanthripp

Electronic Theses, Projects, and Dissertations

Variovorax paradoxus is a Gram-negative aerobic bacterium present in both soil and the human oral microbiome. This species contributes to biotransformation of xenobiotics and promotes plant growth. Interestingly, although the genome of strain EPS lacks the flagellum operon annotated in strain S110, the microbe remains motile, and flagella can be observed microscopically. To investigate this unexpected phenotype, motility mutants were enriched from a Tn5 mutant library using a liquid culture settling strategy. A total of 122 isolates were screened for motility defects, and 57 exhibited significantly reduced motility compared to the wild type. Rescue cloning revealed that over 60% of …

Characterization Of A New Domestic Feline Macrophage Cell Line To Study Molecular Mechanisms Of Feline Coronavirus, Adam Espinoza

Electronic Theses, Projects, and Dissertations

Feline Coronavirus (FCoV) is a veterinary pathogen common in domestic cats. FCoV is transmitted via the fecal-oral route, results in mild enteric symptoms, and is considered feline enteric coronavirus (FECV). During infection the virus may transition from infecting host enteric epithelial cells to infecting host macrophages, resulting in a lethal systemic disease, and the virus is now considered feline infectious peritonitis virus (FIPV). FIPV arises in an infected individual, is not shed in the feces, and is not transmitted from cat to cat. There is limited research defining the host molecular mechanisms driving FCoV lethal outcomes, with few feline cell …

Factors Affecting Diet Selection In Sheep Fed Endophyte-Infected Toxic Fescue, Jordan Thomas

Animal Science Undergraduate Honors Theses

Tall fescue infected with endophyte‑producing fungi can induce fescue toxicosis in grazing ruminants, reducing performance and altering forage intake. Isoflavones—such as biochanin A, a major compound in red clover (Trifolium pratense)—have been proposed to mitigate these effects through improved palatability and vascular relaxation. This study examined whether inclusion of ground red clover (GRC)—either directly or combined with hydroxypropyl methylcellulose (HPMC) and polyethylene glycol (PEG)—affected voluntary diet selection in lambs offered toxic fescue.

Thirty‑two lambs were housed individually and assigned randomly to one of four diets: (1) toxic fescue silage (CONT), (2) silage + 5% GRC (RC5), (3) silage + 10% …

Engineered An Ultrasmall Curcumin Oral Nanoformulation Restores Intestinal Integrity And Gut Microbiota Dysbiosis, Vivek Sharma, Prateeksha Prateeksha, Balwant Paliya, Sateesh Gupta, Sarvendra Singh, Anand Anunay, Sushil Agrahari, Shailendra Singh, Chandana Rao, Saroj Barik, Brahma Singh

Department of Biochemistry and Molecular Biology Faculty Papers

Antibiotic therapy for bacterial infections often disrupts gut microbiota (GM) and intestinal integrity, inducing chronic inflammation and inflammatory bowel diseases. An effective therapeutic intervention is urgently needed to alleviate the aforementioned adverse effects of antibiotics. Curcumin (CUR) reveals great potential to restore intestinal integrity and GM dysbiosis due to its strong antiinflammatory and prebiotic effects. However, the weak solubility and stability of CUR result in limited bioavailability and a short half-life, which restricts its clinical uses. An ultra-small CUR oral nanoformulation was created using a carboxylated galactomannan (cGM), facilitated by hydrogen bonding between the phenolic hydroxyl groups of CUR and …