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Lab-Made 100 Bp Dna Ladder Using Polymerase Chain Reaction And Human Dna, Muhammad Ilmam Bariqi, Zulham Yamamoto, Putri Chalya Firjatu, Luthfi Umam Hakim Nasution, Oryza Sativa Lubis

Makara Journal of Science

Polymerase chain reaction (PCR) is a rapid, molecular biology technique widely used in disease diagnosis and genetic engineering. Conventional PCR products require agarose gel electrophoresis, which employs a DNA ladder as a size reference. Most commercial ladders are plasmid-based and reliable but require additional culture time. We suggest a more efficient method for producing a DNA ladder using DNA derived from human blood. DNA was isolated using a commercial kit. Primer sets generating 100–1000 base pair (bp)-long fragments bearing target regions p12, p13, and p14 were designed using Primer-BLAST. DNA was amplified by routine PCR, visualized on a 1% (w/v) …

Dietary Oil From Hermetia Illucens L. Larvae Promotes Weight Gain And Alters Leukocyte Profiles In Mus Musculus L, Rudy Agung Nugroho Ran, Reni Kurniati, Widya Putri Ambar Sari, Reynaldo Imanuel Sompotan, Retno Aryani, Hetty Manurung, Rudianto Rudianto

Makara Journal of Science

The escalating global demand for high-quality animal-derived proteins has driven the search for sustainable alternatives. Black soldier fly larvae (BSFL; Hermetia illucens L.) oil presents a promising option, by addressing organic waste management while providing nutrient-rich feed. This study evaluated the effects of dietary supplementation with BSFL oil on growth performance, hematological characteristics, and blood lipid profiles in mice (Mus musculus). Male mice were randomly assigned to five groups (n = 5): positive control (fish oil, 5 mL/kg body weight [BW]), negative control (Aquadest, 5 mL/kg BW), and three treatment groups receiving BSFL oil at 2.5, 5, and …

Performance Of Membrane Bioreactor In Nitrogen Removal Using Anammox Bacteria Cultivated From Lake Koto Baru, Zulkarnaini Zulkarnaini, Tiffany Azhra Amanda, Alqadri Asri Putra, Puti Sri Komala, Dewi Nilawati, Widyarani Widyarani, Tarzan Sembiring, Panji Cahya Mawarda, Athanasia Amanda Septevani, Mia Miranti Rustama, Rina Andriyani

Makara Journal of Science

Anaerobic ammonium oxidation (anammox) is the biological conversion of ammonium to nitrogen gas (N₂) using nitrite as an electron acceptor. This study evaluated the performance of a membrane bioreactor (MBR) in nitrogen removal at room temperature using Candidatus Brocadia fulgida, an anammox bacterium previously cultivated from Lake Koto Baru, Tanah Datar, Indonesia. The MBR was operated for 48 days with a hydraulic retention time of 24 h under a continuous supply of ammonium and nitrite, with both substrates provided at influent concentrations of 70, 150, and 250 mg-N/L. Samples were collected twice a week and analyzed via …

Liparis Condylobulbon Reichb. F. From Gumitir, Indonesia: Morphology, Molecular, And Phytochemical Profile, Fuad Bahrul Ulum, Asyifa Yasmin Arum, Kurnia Sa’Adah, Dwi Setyati, Edia Fitria Dwinianti, Ranjithkumar Rajamani, Ari Satia Nugraha, Mukhamad Su’Udi

Makara Journal of Science

Liparis condylobulbon Rchb.f. is an epiphytic orchid across South and Southeast Asia, which holds notable ethnomedicinal and pharmacological significance. This study aims to characterize Indonesian Liparis condylobulbon using integrated morphological, molecular (matK, rbcL, ITS2), and phytochemical (LC-MS, DPPH) analyses of specimens from Mount Gumitir, providing the first comprehensive taxonomic and pharmacological profile to support species authentication and future bioprospecting. The L. condylobulbon specimen displays diagnostic epiphytic morpho-logy, including fusiform pseudobulbs, two elongate leaves, and an erect inflorescence bearing ~123 flowers. Molecular barcoding using matK, rbcL, and ITS2 confirmed its identity, with phylogenetic analyses consistently clustering it with …

Multiplex Functional Assessment Of Variant Effect In The Retinal Transcription Factor Crx, James Lewis Shepherdson

Arts & Sciences Graduate Student Theses and Dissertations

The transcription factor Cone-Rod Homeobox (CRX) is a master regulator of photoreceptor cell fate. Sequence variants in CRX can cause Retinitis Pigmentosa, Cone-Rod Dystrophy, and Leber Congenital Amaurosis, all inherited causes of vision loss and blindness. CRX is the only gene implicated in the pathogenesis of all three of these diseases, which present with both rod- and cone-centric phenotypes of varying age of onset and severity. Several CRX variants have been reported to cause severe dominant disease through antimorphic genetic interactions with wild-type CRX, and yet these mutations are adjacent to variants which are benign or only cause mild, recessive …

New Distributional Record Of Dacus Persicus (Diptera: Tephritidae), A Biocontrol Agent Of Calotropis Procera (Apocynaceae), From Rahim Yar Khan, Punjab, Pakistan, Muhammad Adnan Bodlah, Imran Bodlah, Aleena Kanwal, Alishbah Mohsin, Ayesha Younas, Muhammad Tariq Siddique, Hafiz Muhammad Zia Ullah Ghazali, Muhammad Salman Yousafzai

Journal of Bioresource Management

The tephritid species Dacus persicus Hendel (Diptera: Tephritidae) exhibits strict host specificity to Calotropis species and is known as a destructive seed feeder of Calotropis procera. During field surveys, D. persicus larval stages were obtained from infested fruits of C. procera whereas adults were collected through sweep net sampling. This study documents the occurrence of D. persicus from southern district of Punjab, thereby extending its known distribution within the province beyond previous records from northern Punjab. Adult’s specimens were reared from field collected fruits and net-sweeping. Adults were identified based on detailed morphological examination of external characters and female …

Comparative Morphometric Study Of Saanen And Indigenous Goats, In Northern Pakistan, Nadar Khan, Ihsan Uddin, Sohail Khan, Hayat Ullah Khan, Muhammad Suleman

Journal of Bioresource Management

Goats play an essential role in sustaining rural communities in Pakistan by providing milk, meat, and income under diverse production systems. This study compared the morphometric features of exotic Saanen goats with local breeds (Beetal, Kaghani, Gaddi, and non-descriptive) in Swat, northern Khyber Pakhtunkhwa, to evaluate growth patterns, body structure, and the potential for body weight estimation. A total of 500 animals were sampled from research stations and smallholder farms, with morphometric and qualitative traits recorded following FAO guidelines. Distinct differences among breeds were observed. Beetal goats exhibited the highest body weight (58.2 ± 2.0 kg), chest girth, and body …

Determinants Of Stunting Among Under-Five Children (0–59 Months) In Central Sulawesi Province, Marchell Bamba, Mondastri Korib Sudaryo, Ratna Djuwita, Helwiah Umniyati

Jurnal Epidemiologi Kesehatan Indonesia

Stunting is caused by chronic malnutrition which can be caused by various factors including poverty, poor nutritional intake and maternal health, and inappropriate infant feeding. This study was conducted to determine what factors cause stunting in Central Sulawesi Province. This study was conducted using a cross-sectional observational analysis method using secondary data from the Survei Kesehatan Indonesia (SKI) 2023. The analysis was carried out using logistic regression. The sample used was 2179 respondents. The dependent variable is stunting while the independent variables are age, gender, area of residence, gestational age, provision of PMT, exclusive breastfeeding, maternal occupation, maternal education, and …

The Relationship Between Smartphone Screen Time Duration And Individual Chronotype: A Systematic Literature Review, Diego Sergio Giasia Lumbantobing, Thalia Altamilano Pramudya

Jurnal Epidemiologi Kesehatan Indonesia

The rapid increase in smartphone use over the past decade led to prolonged screen time across age groups, particularly during nighttime hours. Exposure to artificial light and pre-sleep digital activities was associated with circadian rhythm disruption and alterations in individual chronotype. This systematic literature review aimed to synthesize empirical evidence on the relationship between smartphone screen time duration and individual chronotype across all age groups and to identify key moderating factors influencing this association. The review was conducted in accordance with PRISMA 2020 guidelines. Literature searches were performed in PubMed, Scopus, and ScienceDirect for studies published between 2015 and 2025. …

The Effects Of Affordability And Quality Of Care On Utilization Of Primary Healthcare Among Rural Residents In Twifo Ati-Morkwaa District, Ghana: A Qualitative Study, Peter Ansah Boakye, Francis Tei-Nartey, Gideon Owusu

Michigan Tech Publications

Objective: This qualitative study examines how affordability and quality of care influence the utilization of primary healthcare (PHC) services among rural residents in the Twifo Ati-Morkwaa District, Ghana. While Ghana has implemented policies such as CHPS and NHIS to improve access, subjective experiences of rural residents regarding total costs of care and perceived quality remain underexplored. Methods: We conducted a qualitative cross-sectional study between April and September 2024 using purposive and snowball sampling. Ten gender-segregated focus group discussions (FGDs; n = 90 residents) and six in-depth interviews (IDIs) with PHC providers were conducted. Data were collected in Twi, transcribed and …

Fit Check: Making Health Assessments Work For You, Brooklynn Raacke, Ryssa Schlaefli, Caleb Ashley, Makenzie Premer, Brooke Mann, Jessica Phelan

SACAD: Scholarly Activities

When given multiple tools to assess health, people tend to choose the one that is most familiar, fastest, or easiest to interpret. Relying solely on weight can overshadow other important indicators of health, such as body composition, cardiovascular function, and muscular development. Understanding this preference, even when other tools are available, matters for both educators and fitness professionals. This study used naturalistic observation to assess gym-goers' usage of health and anthropometric assessment tools. Results indicated that while gym-goers initially showed interest in less commonly used assessment tools, they overwhelmingly used the scale. Implications, limitations, and future directions are discussed.

Humin-Based Bioadhesives For Manufacturing Particleboard Panels, Papiya Bhowmik, Reza Hosseinpourpia

Michigan Tech Publications

This study focused on a novel approach to combine humin, derived from fructose-based biorefinery processes, with two-dimensional graphene nanoparticles and glyoxal, a bio-based crosslinker, to formulate functional bioadhesives for manufacturing particleboards. Five different single-layer 8 mm particleboard panels were prepared using the developed humin-based adhesive formulas with and without graphene and tested by means of their mechanical properties and thickness swelling in accordance with the EN 310 and EN 319standards. The results were compared with panels bonded with commercial melamine-urea-formaldehyde (MUF), showing that the mechanical properties of particleboards bonded with humin reinforced with graphene nanoparticles were almost comparable to those …

Assessment Of Fecal Indicator Bacteria And Vendors' Handling Practices For Safety Of Salads And Raw Leafy Vegetables In Dar Es Salaam, Tanzania, Gloria B. Mrisho, Thomas J. Lyimo, Godwill D. Mrema

Tanzania Journal of Science

Fresh leafy vegetables have gained popularity in Tanzanian cities due to their nutritional benefits and increased promotion by health practitioners. Assessment of their microbiological quality and safety is essential for determination of associated health risks. This study enumerated Fecal Indicator Bacteria (FIB) for determination of microbiological quality and safety of fresh leafy vegetable salads sold by street vendors and raw leafy vegetables obtained from Sinza market in Dar es Salaam. Handling practices, awareness and consumer satisfaction on the safety of leafy vegetable salads was also assessed among salad street vendors and consumers. Samples were collected from four stations and analyzed …

An Atlas Of Genetic Effects On The Monocyte Methylome Across European And African Populations, Wanheng Zhang, Chuan Qiu, Xiao Zhang, Zichen Zhang, Kuan-Jui Su, Zhe Luo, Minghui Liu, Bingxin Zhao, Lang Wu, Qing Tian, Hui Shen, Chong Wu, Hong-Wen Deng

Faculty, Staff and Student Publications

No abstract provided.

Reproductive Performance And Its Association With Esr, Prlr, Fshb Genes In Indigenous “I” Pigs, Do Duc Luc, Phan Thi Tuoi, Nguyen Thai Anh, Do Thi Hue, Nguyen Hoang Thinh

The Thai Journal of Veterinary Medicine

Improving the reproductive performance of sows plays an important role in the pig industry, and it applies not only to commercial pigs but also to indigenous pigs. In this study, the polymorphisms of three genes, namely estrogen receptor (ESR), prolactin receptor (PRLR), and follicle-stimulating hormone beta subunit (FSHB) in the “I” pig population were investigated, and their associations with reproductive performance were evaluated. Ear tissue samples were collected from 151 fattening pigs and 126 sows across breeding farms in Vietnam for polymorphisms of the ESR, PRLR and FSHB genes. Three genotypes (AA, AB, and BB) were detected at the …

Norfolk Flooding And Its Effect On Nutrient And Enterococcus Levels, Malachi R. Stephens

Knowledge and Creativity Expo

Tidal flooding is common in the City of Norfolk, VA due to high rates of sea rise in the region, as well as the region’s subsidence. This has led to increases in flooding at high tide, especially during the spring tide portion of the tidal cycle.  At high tide water from the Elizabeth and Lafayette Rivers can flood the landscape directly or can rise out of storm drains. This floodwater often stays on land for long periods of time and can deliver contaminants back into the rivers. To determine the amounts of nutrients and Enterococcus, a fecal indicator, delivered to …

Fortification Of Flour Cultivated In The Territory Of The Republic Of Uzbekistan With Own Iron And Zinc, Nodira B. Kurbanova, Gulnoza Z. Djakhangirova, Bakhtiyar I. Kurbanov, Dase D. Klava

CHEMISTRY AND CHEMICAL ENGINEERING

The aim of the study is to investigate the regional characteristics of iron and zinc content in wheat and flour produced in Uzbekistan using instrumental neutron activation analysis, and on this basis to provide a scientific justification for effective approaches to flour fortification and the preservation of micronutrients during grain processing. The study analyzed wheat flour produced from grain grown in five regions of Uzbekistan (Khorezm, Surkhandarya, Bukhara, Tashkent, and Fergana). Instrumental Neutron Activation Analysis (INAA) was applied for the first time in Uzbekistan to comprehensively assess the microelement composition of flour. It was found that fortification using natural sources …

Preparation Of Semi-Hard Cacciota Cheese From Local Milk And Analysis Of Its Physicochemical And Microbiological Properties, Shahnoza U. Yuldasheva, Dilshod P. Rakhimov, Dilrabo Q. Maksumova, Shahlo I. Akhmedova, Asaloy U. Narimonova

CHEMISTRY AND CHEMICAL ENGINEERING

Including cheese products in daily diet will give tangible results, enriching body with nutrients rich in high-quality proteins. The aim of the research is to create an Italian semi-hard cheese, caciotta, from local cow's milk. In the experiment, pepsin of the CHY-MAX® Powder Plus NB brand was used. The resulting product contained 1.46 mg/kg zinc (Zn), which is approximately 34 times lower than the permissible limit (50 mg/kg), which is considered a high quality indicator. From a biological safety point of view, no aflatoxin M1 was detected in the cheese (0.0 mg/kg), indicating the absence of mycotoxin contamination of cow's …

Effect Of Variety And Seaweed Extract Spray In The Content Of Some Medically Active Phenolic Compounds In Grape Fruits, Ali Ahmed Hussein Almyali, Salim Kadhim, Ali S. Hassoon, Ameer Al Fatlawy

Al-Bahir

Grape (Vitis vinifera L.) is one of the most economically significant fruit crops which has a large adaptive range, nutrition and medicine effect. A variety of 70 types of grapes are planted on more than 11.6 million plants in Iraq, generating a total annual output of more than 421,000 tons. Increasing grape quality, and particularly the phenolic composition recently has been of major interest. Leaf nutrition – particularly seaweed extracts, being rich in amino acids, trace elements and growth promoting substances – has proved to be a potential tool to enhance plant growth and fruit biochemical quality. The …

From Fair To Cure: Guidelines For Computational Models Of Biological Systems, Herbert M. Sauro, Eran Agmon, Michael L. Blinov, John H. Gennari, Joseph L. Hellerstein, Adel Heydarabadipour, Bartholomew E. Jardine, Elebeoba May, David P. Nickerson, Lucian P. Smith, Gary D. Bader, Frank T. Bergmann, Patrick M. Boyle, Andreas Dräger, James R. Faeder, Song Feng, Juliana Freire, Fabian Fröhlich, James A. Glazier, Thomas E. Gorochowski, Tomas Helikar, Henning Hermjakob, Stefan Hoops, Peter Hunter, Princess I. Imoukhuede, Sarah M. Keating, Matthias König, Reinhard Laubenbacher, Leslie M. Loew, Carlos F. Lopez, William W. Lytton, Rahuman S. Malik-Sheriff, Andrew Mcculloch, Pedro Mendes, Lealem Mulugeta, Chris J. Myers, Jerry G. Myers, Anna Niarakis, David D. Van Niekerk, Brett G. Olivier, Alexander A. Patrie, Ellen M. Quardokus, Nicole Radde, Johann M. Rohwer, Sven Sahle, James C. Schaff, Falk Schreiber, T. J. Sego, Janis Shin, Jacky L. Snoep, Rajanikanth Vadigepalli, H. Steven Wiley, Dagmar Waltemath, Ion I. Moraru

Computational Medicine Center Faculty Papers

Guidelines for managing scientific data have been established under the FAIR principles, requiring that data be Findable, Accessible, Interoperable, and Reusable. In many scientific disciplines, especially computational biology, both data and models are key to progress. For this reason, and recognizing that such models are a very special type of "data", we argue that computational models, especially mechanistic models prevalent in medicine, physiology and systems biology, deserve a complementary set of guidelines. We propose the CURE principles, emphasizing that models should be Credible, Understandable, Reproducible, and Extensible. We delve into each principle, discussing verification, validation, and uncertainty quantification for model …

3'Utr Mapping Reveals Alternative Polyadenylation In Right Ventricular Failure, Rahul Neupane, Kartiga Natarajan, Erik E Suarez, Henry J Pownall, Kai-Lieh Huang, Eric J Wagner, Ajay Kumar Verma, Rambabu Majji, Hari Krishna Yalamanchili, Ashrith Guha, Rajarajan A Thandavarayan

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Assessing The Impact Of Lampenflora Growth On Invertebrate Distribution And Food Resource Use In Ryusen-Do Cave, Japan, Kazuhide Nakajima, Kanato Ando, Shuji Watanabe, Masuo Komukai

International Journal of Speleology

Subterranean spaces, such as caves with openings to the surface, represent unique environments characterized by limited resources derived from photosynthetic primary production. Consequently, organic inputs from organisms inhabiting above- and belowground habitats, such as bats, play a crucial role in maintaining cave ecosystems. However, in show caves converted into tourist attractions, environmental changes have progressed without sufficient ecological assessment. One important factor is the impact of photosynthetic organisms colonizing around artificial lights, known as lampenflora, on cave ecosystems. Lampenflora growth may influence invertebrate distribution and resource use; however, its impact on both aspects has been poorly evaluated. In this study, …

Comparative Effect Of Mineral, Organic, And Mixed Sunscreens Formulations On The Life History Of A Benthic Marine Invertebrate, Jacob Anthony Fox, Courtney Lott, Araceli Rodriguez-Romero, Gloria Massamba N'Siala

Knowledge and Creativity Expo

Mineral and organic sunscreens are emerging anthropogenic pollutants in coastal marine waters and have been shown to affect marine invertebrates via genotoxicity, endocrine disruption, oxidative stress, and impacts on life-history traits, such as reduced survival, growth, and fecundity. Most experiments expose marine organisms to isolated UV filters, with fewer studies testing complete formulations, co-occurring exposure to organic and mineral sunscreens, or the modulating influence of format type on toxicity. In this experiment, we tested the impact of organic sunscreens (3% Avobenzone, 15% Homosalate, 5% Octinoxate, and 10% Octocylene) and 24% zinc oxide mineral sunscreens on the survival and fecundity of …

Effects Of Alk2 Deficiency On T Cell Fate And Regulatory T Cell Populations, Jalyn Reeves, Afia Salsabil Alam

Knowledge and Creativity Expo

Regulatory T cells (Tregs) are central to immune tolerance, and their lineage stability is established during thymic development and maintained in the periphery through tightly regulated signaling networks. Members of the BMP receptor family have been implicated in T-cell differentiation, yet the specific role of the type I receptor ALK2 in shaping cell fate and sustaining the regulatory program remains unclear. To examine how ALK2 contributes to T-cell development, we generated ALK2-knockout murine chimeras and analyzed lymphoid populations from the thymus, spleen, and peripheral tissues. Thymocytes and lymphocytes were isolated and stained with antibody panels to identify developmental subsets and …

Interview With Orca Award Winner David Hage, Unl Chemistry; Drug-Protein Interactions, Mark Griep, David Hage

UNL ORCA Interview Series

Prof. David Hage was awarded the ORCA in 2018. He was born in La Crosse, Wisconsin, and earned a bachelor’s in chemistry at the University of Wisconsin in La Crosse. Next, he earned a doctorate from Iowa State University and did postdoctoral work at the Mayo Clinic in Rochester, Minnesota. In 1989, he joined UNL as an assistant professor of chemistry and quickly rose through the ranks. He has won many awards and, for the past 13 years, has been the James Hewet University Professor of Chemistry. Allow me to share some remarkable facts about Dave before we get into …

Variable Responses To Surface Washing Methods Used On Euplatypus Compositus (Curculionidae: Platypodinae) Prior To Fungal Isolation, Connor Biser, Jiri Hulcr, Miranda Barnes, Seth Bybee

Library/Life Sciences Undergraduate Poster Competition 2026

Ambrosia beetles (Platypodinae and Scolytinae) are a group of weevils that burrow into the xylem of trees and inoculate symbiotic fungi. These fungi grow in the walls of their galleries and serves as the beetles’ food source.1 [Fig. 1] This system of symbiotic interaction is highly convergent, and some beetles carry plant pathogens.2 Understanding the community of fungi associated with ambrosia beetles can help us assess forest risks and describe evolutionary patterns. When isolating fungi from beetles, researchers often include a preliminary surface washing step to limit superficial contaminants from the surface of the beetle. There have been no comparisons …

Unlikely Relationships: Plant Growth Promotion With Fish Waste Microbes, Thomas W. Scharf, Brad Geary

Library/Life Sciences Undergraduate Poster Competition 2026

1st Place - Open House Presentation

• Aiding plant growth is becoming more important as climate conditions continue changing in key wildlands and farmlands.
• Future Fresh Farms has created a product from fish waste, addressing this need.
• This study aimed to compare the outcomes of plants grown with MicroRevive, derived from fish waste, against plants grown with other growth promoting bacteria (PGPB).

Uncovering The Roles Of Environment And Phylogeny In The Expression Of A Shared Morphological Trait In Squamates, Austin Rejcek, Dylan Mcmullen, Logan Gren, Randy L. Klabacka

Library/Life Sciences Undergraduate Poster Competition 2026

Addressing the roles of adaptive and nonadaptive processes in trait evolution is a major focus of evolutionary biology

Recently, we observed an undescribed morphological trait on the eyelids of squamate lizards - AKA ''Palpebral combs'' (See photos)

This trait appears to be widespread within lguania, although there is consistent morphological variation between species of varying ecologies

Uav Imaging: Reliability Of Drone-Captured Images In Ecological Work, Andrew Sevy, Steven L. Petersen, Russell Torgersen, Otto De Grof, Austin Housley

Library/Life Sciences Undergraduate Poster Competition 2026

Unmanned Aerial Vehicle (UAV)-based imagery offers a scalable, repeatable method for surveying large landscapes, with the potential to reduce field effort while increasing detection consistency.

Despite growing interest in UAV applications, the accuracy of drones in identifying small, irregular features like stud piles remains poorly quantified, creating uncertainty about their reliability for management decisions.

Three Lower Extremity Muscle Volumes Predict Countermovement Jump Height In Collegiate Basketball Players, Josh Vandenakker, Jacob Green, Malorie Wilwand, Micahel Davie, Steven D. Arnold, A. Wayne Johnson

Library/Life Sciences Undergraduate Poster Competition 2026

The countermovement jump (CMJ) is an essential ability in basketball, and increased jump height provides players with a competitive advantage.

Sport-specific data connecting MRI-derived muscle volumes to CMJ mechanics and height remains limited.

Our aim: To examine correlations between CMJ height and the muscle volume of the soleus, quadriceps, and gluteus maximus in collegiate basketball players.