Digital Commons Network^™

Analyzing The Effects Of Message Testing On The Public's Opinion About Gene Editing In Agriculture And Medicine, Luke Sanford Wimpy

Graduate Theses and Dissertations

This paper will present results from the final survey of a multi-stage project examining factors that influence opinions about gene editing in agriculture and medicine. In this final survey, 3,200 U.S. respondents were recruited and randomized to view one of eight videos that communicated the safety of gene editing to determine which factors are most influential in changing opinions. The eight videos were designed based on data collected from focus groups and three previous surveys in this multi-stage project. The eight videos varied by three attributes: messenger, message, and application. The messenger in the videos was either a researcher or …

Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser

Biology Graduate Theses

Over the past several decades, cold- and cool-water fish populations across the Upper Midwest, including Minnesota and Wisconsin, have shown troubling signs of decline. Among the most affected is the walleye/Ogaa (Sander vitreus), a species deeply embedded in the ecological balance, cultural identity, and economic vitality of the region, and central to the lifeways, treaty-reserved rights, and resource stewardship of Tribal Nations, including Ojibwe communities across the Upper Midwest. Despite ongoing stocking efforts and harvest regulations, many lake systems have experienced persistent declines in natural recruitment, prompting resource agencies to investigate the underlying causes. One such system is …

Evaluating Management Strategies And Technology To Improve Grazing Efficiency, Thomas E. Aquino

Department of Animal Science: Dissertations, Theses, and Student Research

Increasing land costs and labor constraints continue to challenge beef producers. More intensive practices can increase harvest efficiency; however, many producers are deterred from implementing them due to increased labor and management considerations. Virtual fencing (VF) may provide a reduced labor alternative to traditional fencing for managing intensive grazing. This dissertation evaluated management strategies and VF technology to improve grazing efficiency of stocker cattle systems. In Experiment 1, VF was used to effectively manage groups of steers grazing in adjacent paddocks separated by only virtual boundaries. There was no difference in VF containment (99%) when steers were grazing co-fenced, with …

Evaluation Of Palm Oil As An Ingredient In Finishing Beef Cattle Diets On Performance, Carcass Characteristics, And Meat Quality, And The Composition Of Spayed Heifers To Intact Heifers Fed Melengestrol Acetate On Feedlot Performance And Carcass Characteristics, Tate S. Johnson

Department of Animal Science: Dissertations, Theses, and Student Research

Heifers represent a large proportion of cattle placed in feedlots, making pregnancy management an important consideration for maintaining performance and carcass value. Melengestrol acetate (MGA) is commonly included in feedlot diets to suppress estrus and prevent pregnancy in intact heifers during the finishing period. Alternatively, spaying has historically been utilized as a management practice to allow heifers to graze alongside breeding cattle without the risk of pregnancy. Despite its historical use, there is limited contemporary research comparing the growth performance and carcass characteristics of spayed heifers with intact heifers managed with MGA under modern feedlot conditions. Therefore, a commercial study …

Optimized Ultrasound Imaging Of Phase-Change Nanodroplets, Charles R Dyall, Dmitry Nevozhay, Andy Liu, Trevor M Mitcham, George J Lu, Konstantin V Sokolov, Richard R Bouchard

Faculty, Staff and Student Publications

Phase-change nanodroplets (PCNDs) continue to generate significant research interest due to their potential to extravasate into tissue, to be targeted for molecular imaging and drug delivery, and to undergo an induced phase-change to "activated" microbubbles (MBs) for ultrasound (US) imaging. To accurately quantify molecular markers, however, one assumes a consistent proportion of PCNDs in a region of interest (ROI) are stably activated and imaged. Herein we present a framework for developing a diagnostic sequence that is optimized for PCND activation uniformity, contrast, and acquisition time. To develop this framework, activation was examined at three scales of increasing complexity: single, adjacent, …

Corrigendum To 'Risk Factors For Bronchiolitis Obliterans Syndrome After Initial Detection Of Pulmonary Impairment After Hematopoietic Cell Transplantation' [Transplantation And Cellular Therapy 29/3 (2023) 204-204], Mansour Alkhunaizi, Badar Patel, Luis Bueno, Neel Bhan, Tahreem Ahmed, Muhammad H Arain, Rima Saliba, Gabriela Rondon, Burton F Dickey, Lara Bashoura, David E Ost, Liang Li, Shikun Wang, Elizabeth Shpall, Richard E Champlin, Rohtesh Mehta, Uday R Popat, Chitra Hosing, Amin M Alousi, Ajay Sheshadri

Faculty, Staff and Student Publications

No abstract provided.

Potential Impact Of Parasites In The Transmission Of Chronic Wasting Disease, Paulina Soto, Rodrigo Morales

Faculty, Staff and Student Publications

No abstract provided.

Growth Hormone Pathway As A Prognostic And Therapeutic Biomarker In Patients With Unresectable Hepatocellular Carcinoma Treated With Radiation Therapy, Joe R Eid, Safa Kaseb, Lianchun Xiao, Ryan Sun, Mahesh Kumar Kannan, Manal Hassan, Asif Rashid, Hop S Tran Cao, Hesham M Amin, Eugene J Koay

Faculty, Staff and Student Publications

Purpose: Radiation therapy (RT) has emerged as an effective local therapy option for patients with unresectable hepatocellular carcinoma (HCC) and is associated with improved overall survival (OS). However, some patients still have poor outcomes after RT, and biomarkers are needed to stratify prognostic groups. High circulating growth hormone (GH) levels promote HCC proliferation and survival and are associated with aggressive disease. In this study, we evaluated whether pretreatment GH levels are associated with OS and progression-free survival (PFS) in patients who underwent RT for unresectable HCC.

Methods and materials: Patients undergoing RT for HCC during 2017-2023 were identified from a …

Decomposition Study Using Pigs In Southeastern Nebraska During The Fall And Winter Seasons, Ryan Bodine

Department of Anthropology: Theses and Student Research

When an individual is found deceased, one of the tools used in the identification process is estimating the post-mortem interval (PMI). This estimate is used to determine how long an individual has been deceased once their remains are found. To estimate an accurate PMI, knowing how decomposition is affected by external factors is key. The transition period from fall to winter in southeastern Nebraska consists of fluctuating temperatures as the seasons transfer from fall to winter. This thesis investigates how decomposition is affected during this transition period while also looking at two different environments that are common in Nebraska, prairies …

Exploring The Biocultural Profile Of The Undocumented Populations Of The Sonoran Corridor: A Skeletal Assessment Of The Prevention Through Deterrence Strategy, Mary A. Vaughn

Department of Anthropology: Theses and Student Research

Over the last thirty years the mortality rate of the undocumented population at the US-Mexico border has risen, requiring methods distinguishing US-born individuals from immigrants to improve the likelihood of identification and repatriation through foreign consulates. Forensic anthropologists with the Pima County Office of the Medical Examiner (PCOME) have advocated for the use of the biocultural profile, combining the biological profile with factors including oral health, osteological stress, and personal effects. The biocultural profile serves two purposes, functioning both as a tool for identification and with which to document structural inequality within communities of origin.

This study examines changes in …

Intraday Cotton Futures Price Responses To World Agricultural Supply And Demand Estimates (Wasde) News, Owen Mclane Tyrone

Graduate Theses and Dissertations

Price discovery in agricultural futures markets is augmented by information about supply and demand, leading markets to react when public reports are released. Effective price discovery in futures markets is vital to allocate physical commodities across time, synthesize information, and provide hedging tools. Price discovery in cotton markets is unique because it is less liquid than many other commodity markets. Therefore, our objective is to measure the initial price response in the cotton futures market to unanticipated ending stocks information in the WASDE report; then determine if markets continue to adjust after the initial price change. To meet our objective, …

Nadh Flim Reveals Cellular Bioenergetics And Mitochondrial Morphology During Drosophila Convergent Extension, Maria Espana Pena

Graduate Theses and Dissertations

Mitochondria are dynamic organelles that can fragment or fuse to support different bioenergetic demands and cellular processes, although the role of mitochondrial fission and fusion during embryonic development is not well understood. Fluorescence lifetime imaging (FLIM) of the mitochondrial cofactor NADH can be used to visualize mitochondrial networks and infer aspects of cellular bioenergetics in a label-free manner. We used NADH FLIM to test whether germband cells undergo changes in cellular metabolism during Drosophila convergent extension (CE)––a process in which hundreds of epithelial cells undergo coordinated intercalation to elongate the embryo. Contrary to our expectations, we did not observe significant …

Exploring Agricultural Pathways: An Analysis Of Arkansas Community Colleges, Natasha Hightower

Graduate Theses and Dissertations

As demand for a highly skilled agricultural workforce continues to grow, Arkansas must strengthen accessible and clearly defined academic and career pathways to prepare future agricultural professionals. Community colleges serve as a critical entry point into higher education, particularly for rural and first-generation students, and play a vital role in both workforce development and transfer preparation. However, information related to agricultural education offerings, transfer pathways, and faculty at Arkansas community colleges is often decentralized and difficult for students and stakeholders to locate. The purpose of this study was to examine agricultural education offerings at community colleges in Arkansas, specifically agricultural …

Validation Of Rice Tissue Sampling For In-Season Nutrient Management Of Nitrogen, Phosphorus, And Potassium, Tanner Smith

Graduate Theses and Dissertations

Nitrogen (N), phosphorus (P), and potassium (K) are the 3 most important nutrients involved in rice (Oryza sativa L.) growth and development. Tissue sampling has been proven to be a potential tool to provide inseason information about plant nutrient concentrations at specified timings and growth stages. With increased interest in tissue sampling and improving nutrient management effectiveness, more information is needed to validate plant tissue concentrations at which additional fertilizer inputs may be warranted. Experiments were conducted in 2024 and 2025 at the Rice Research and Extension Center (RREC) near Stuttgart, Ark.; Pine Tree Research Station (PTRS) near Colt, Ark.; …

Genomic Surveillance Of Guillain–Barré Syndrome-Associated Genes In Campylobacter Jejuni Across Human And Non-Human Reservoirs, Emma Doughty

Biological Sciences

Campylobacter jejuni is a leading cause of foodborne illness and a major trigger of Guillain–Barré Syndrome (GBS), an autoimmune neuropathy caused by molecular mimicry between bacterial lipooligosaccharides (LOS) and host nerve gangliosides. While prior genomic studies have focused primarily on human clinical isolates, the prevalence and distribution of GBS-associated genes in non-human reservoirs remain poorly characterized. This study used genomic datasets derived from analyses of human and non-human C. jejuni isolates to assess the distribution of GBS-associated genes, with a focus on the neuABC sialylation pathway. Results demonstrate that these genes are present in both host types, across multiple geographic …

Alternative Abl1 Isoform Expression In Oncogenic Translocations: Implications For Cancer Progression, Jessica Teague

Biological Sciences

Abstract (also on poster): Previous studies in our lab show that an alternate isoform of Bruton’s tyrosine kinase, BTK-C, is required for the survival of several epithelial-derived cancer cells. BTK-C is transcribed from an alternative promoter and includes a 34-amino-acid N- terminal extension with a palmitoylation sequence that alters its subcellular localization. Nine other human kinases have similar alternative N-termini (ANT), often producing palmitoylated or non-palmitoylated isoforms from different transcriptional start sites. Among these, ABL1 is a known oncogenic kinase and key drug target. Translocation mutations at the 5′ end of ABL1 are a major cause of adult leukemias. This …

Solvent-Dependent Proteome Profiling In Edible Mushrooms (Hypsizygus Marmoreus And Agaricus Bisporus), Tanvir Ahmed, O'Lone, Angéla Juhász, Utpal Bose, Kenneth U. Agbo, Netsanet Shiferaw Terefe, Michelle L. Colgrave

Research outputs 2022 to 2026

This study compared five solvents - urea, carbonate-bicarbonate, citrate, phosphate, and water - for their ability to extract proteins from beech (BM) (Hypsizygus marmoreus) and Swiss brown (SB) (Agaricus bisporus) mushrooms. Using mass spectrometry-based proteomics and bioinformatics, we found that urea produced the highest total protein identifications by data-dependent acquisition at 1% false discovery rate (FDR): BM: 1739, SB: 1907; and by data-independent acquisition: BM: 1969, SB: 2172 proteins, especially enriching cytosolic and ribosomal proteins. Carbonate-bicarbonate favoured the extraction of metabolic and translational proteins, while citrate targeted extracellular and membrane-associated proteins related to cell wall dynamics. Phosphate-enriched catalytically active proteins, …

Donor And Recipient Microbiome Interactions Determine Functional Outcomes Of Fecal Microbiota Transplantation For Clostridioides Difficile Infection, Sophie A. Millard

All Dissertations

Clostridioides difficile is a major cause of healthcare-associated diarrhea. Infection typically occurs after antibiotic treatment disrupts the gut microbiome, allowing C. difficile to colonize and produce toxins leading to disease (CDI). Standard antibiotic treatments can resolve acute infection but frequently lead to recurrence, highlighting the need for alternative strategies that restore the gut ecosystem. Fecal microbiota transplantation (FMT), the transfer of stool from a healthy donor, has emerged as an effective therapy for recurrent C. difficile infection (rCDI). Understanding the mechanisms underlying the success of FMT for treatment of rCDI could lead to safer, more defined microbial mixtures.

This dissertation …

High Throughput Phenomics Pipeline For Pulse Crop Nutritional Breeding, Amod Udayanga Madurapperumage

All Dissertations

Dry pea (Pisum sativum L.), lentil (Lens culinaris Medik.), and chickpea (Cicer arietinum L.) are major pulse crops valued for their high nutritional composition and importance to global food systems. Pulses are rich in carbohydrates, protein, and essential minerals, making them ideal whole foods and critical contributors to food and nutrition security. Due to these advantages, pulse breeding programs are increasingly focusing on enhancing nutritional traits, such as protein quality, amino acid balance, and micronutrient density, through the process of biofortification. However, improvement of agronomic traits remains equally essential. Characteristics such as plant height, standability, stress tolerance, …

Integrating Incentive Design And Spatial Prioritization For Climate-Smart Forestry Adoption In South Carolina, United States, Miah Maye Pormon

All Dissertations

Forests provide essential ecosystem services, including carbon sequestration, water regulation, timber production, and habitat provision. However, increasing development pressures and land-use changes threaten forest persistence and long-run ecosystem service provision. Climate-smart forestry (CSF) practices, such as improved forest management and extended rotation, offer opportunities to enhance carbon storage, forest resilience, and economic livelihoods. The effectiveness of these practices depends on forest owners’ participation and the strategic allocation of financial resources or incentives. This dissertation develops an integrated framework that combines behavioral economic analysis and mapping to improve the design of current incentive programs. The first chapter employs the Contingent Valuation …

Recovery Timeframe Is A Critical Component In Preserving Carbon Stocks In Disturbed Tropical Seagrass Meadows, Taylor Renee Condron, Timothy M. Smith, Paul H. York, Nicholas J. Murray, Pere Masque, Michael A. Rasheed

Research outputs 2022 to 2026

Seagrass meadows, though covering a small percentage of the ocean floor, play a critical role in marine carbon sequestration. However, the mechanisms behind this role remain uncertain due to challenges in mapping seagrass extent and flux, accounting for spatial variation in carbon storage, and incorporating site-specific histories. This study investigates the effects of short-term (≤10 years) seagrass loss on sediment carbon stocks within a large seagrass meadow in the Great Barrier Reef region. Using remote sensing data to construct a seagrass recovery timeline, combined with field sampling and assessments of ecological factors, we found higher sediment organic carbon in areas …

The Rise And Fall Of The World's Greatest Marine Biodiversity Hotspot, Alexandre C. Siqueira, Kiessling, Nussaïbah Raja, David R. Bellwood

Research outputs 2022 to 2026

Habitat condition and area shape global species distributions, with shallow-water reefs hosting a disproportional share of marine biodiversity. Although reef area is a well-established predictor of marine species richness, its historical context is less well understood. Here, we show that the rise of tropical marine biodiversity is closely tied to reef expansion in space and time. During the Early/Mid Miocene (23 to 11.6 million years ago), Indo-Pacific reefs reached unprecedented size and thickness, surpassing any reef systems in the past 66 million years. These massive reefs, likely driven by unique environmental, biotic, and tectonic conditions, fostered the expanding diversity and …

Comparing Microbial Communities Of Diseased And Healthy Isopora Palifera Corals And Adjacent Waters At The Cocos (Keeling) Islands, Sophie Preston, Jacquelyn Jones, Megan J. Huggett, Arne A.S. Adam, Nicole E. White, Kar Chun Tan, Zoe Richards

Research outputs 2022 to 2026

Growth anomalies (GAs) are coral diseases characterised by tumour-like skeletal lesions reported globally, yet their causes remain poorly understood. Microorganisms are integral to coral health, but the role of bacterial communities in GAs remains unclear. We investigated an outbreak of GAs in Isopora palifera at the Cocos (Keeling) Islands using 16S rRNA amplicon sequencing to compare bacterial communities of GA-affected and asymptomatic corals, surrounding water and potential pollution sources. Significant differences in bacterial beta diversity were observed across sites, with an interaction between location and coral health status. Coral and water samples hosted distinct microbial communities, but there was no …

Arkansas Soybean Research Studies 2024, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

The 2024 Arkansas Soybean Research Studies includes research reports on topics pertaining to soybean across several disciplines from breeding to post-harvest processing. Research reports contained in this publication may represent preliminary or only data from a single year or limited results; therefore, these results should not be used as a basis for long-term recommendations. Several research reports in this publication will appear in other University of Arkansas Division of Agriculture’s Arkansas Agricultural Experiment Station publications. This duplication is the result of the overlap in research coverage between disciplines and our effort to inform Arkansas soybean producers of the research being …

Impact Of Inhibiting Mitochondrial Calcium Uptake On Calcium Homeostasis And Postmortem Metabolism In Porcine Muscle, Fatema Rahaman Mishu

All Graduate Theses and Dissertations, Fall 2023 to Present

After harvesting the animal, pork muscle continues to undergo changes as it turns from muscle into meat. During this early period, the muscle uses up energy and produces acid, which lowers pH and can affect important quality traits such as color and how well the meat holds water. A key driver of these early changes is calcium, a natural mineral inside muscle cells that helps control contraction and energy use. In this study, we tested whether mitochondria (the cell’s “powerhouse”) help control calcium after harvest and, in turn, influence early postmortem changes in pork. We used a research compound (DS16570511) …

Alfalfa Yield, Quality, And Nutrient Use Response To Pivot Sprinkler Technology, Deficit Irrigation, And Genetics, Dakota Boren

All Graduate Theses and Dissertations, Fall 2023 to Present

In water limited environments, alfalfa is often criticized for its high water use, prompting implementation of new irrigation technologies, deficit irrigation, and adapted crop genetics. Cumulative potential benefits from combining, or stacking, these water-optimizing practices have not been identified. The objective of this on-farm research study was to determine how irrigation technologies, deficit irrigation, genetics, and combinations of these practices impact alfalfa yield and forage quality. Trials were conducted at three sites in Utah from 2020 to 2022 on young alfalfa stands (establishment and first one to three production years). Treatments included five irrigation technologies, four irrigation rates, and two …

Effects Of Serum And Commercial Serum-Free Media On Development Of In Vitro Produced (Ivp) Embryos In Cattle, Kyla Miller

All Graduate Theses and Dissertations, Fall 2023 to Present

Serum-containing media has been shown to increase incidence of large offspring syndrome and to alter gene expression, protein synthesis, and cell signaling. These factors together can result in increased pregnancy loss when combined with assisted reproduction technologies. However, there are very few direct comparisons of commercial serum-free media (IVF Bioscience™) and serum-containing media on both IVF and cloned embryo development in cattle. The aim of this study was to determine the role of serum in oocyte maturation and subsequent embryo development. Three culture systems, including in vitro maturation (IVM) and in vitro culture (IVC), were used in this study. Both …

Effects Of Mineral Supplementation On Ovine Performance, Forage Selectivity, And Rangeland Utilization In Southern Utah, Ryan A. Spurling

All Graduate Theses and Dissertations, Fall 2023 to Present

Sheep producers in the Intermountain West region of the US rely on productive rangeland ecosystems that vary widely in forage composition and nutrient availability. As such, supplementation is often necessary to offset dietary deficiencies. Soils and forages grown on rangelands are known to be highly variable in mineral content. Even so, a third to half of the producers in the Intermountain West choose not to provide a complete mineral supplement. Rangeland environments are often characterized by rugged geographical features that make optimal supplement placement difficult. New advances in technology have allowed us to measure individual animal intake on rangelands via …

Unravelling Taxonomic Complexity In Elusive Cetaceans: Mitogenome Insights Into Evolutionary History And Cryptic Diversity Of Bryde's Whales, Dominique Paynee, Paulette Bloomer, Gwenith Penry, Simon Elwen, Ada Natoli, Vaisakh Dhanalakshmy, Thyago Cardoso, Els Vermeulen

All Works

Cetaceans, despite their ability to cross wide distances, enabling mostly high levels of gene flow, can develop unique genetic lineages driven by variations in life-history traits, diverse migratory routes and local adaptation, complicating taxonomy and delineation of conservation units. Comprehensive genetic data are essential to overcome the challenges of obtaining suitable sample sizes from wild cetaceans while untangling cryptic species diversity. The Bryde's whale (Balaenoptera edeni) has a complicated discovery and taxonomic history that hinders accurate conservation assessments. In southern Africa, two populations are recognised: the inshore (SAi) and the offshore southeast Atlantic population (SEA). This study examines their phylogenetic …

Understanding Impact Of Redox Potential On Microbial Community Of Cheddar Cheese During Aging, Abrianna Y. Paez

All Graduate Theses and Dissertations, Fall 2023 to Present

Cheese ripening is a complex process that shapes the flavor, texture, and overall quality of cheese, yet many of the factors that control how these changes occur are still not fully understood. One factor that may play an important role is redox potential, which describes the balance between oxidation and reduction reactions happening inside the cheese. These reactions can influence both the activity of bacteria and the chemical processes that create flavor. Past efforts to control redox using chemical additives often harmed beneficial bacteria, limiting their usefulness.

In this study, we tested the use of mechanical disruption and gas exposure …